Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,800,657 T→C E10G (GAG→GGG)  rfaI ← UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,800,6570TC99.0% 634.7 / ‑3.2 201E10G (GAG→GGG) rfaIUDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (115/84);  total (115/86)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

Only 100 of 205 total aligned reads displayed.

AGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATT  >  NC_000913/3800567‑3800746
                                                                                          |                                                                                         
aGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCa                                                                                         <  2:1851457/93‑1 (MQ=255)
 gAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAg                                                                                        <  1:1014286/93‑1 (MQ=255)
 gAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAg                                                                                        <  2:4400405/93‑1 (MQ=255)
   aaGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGtt                                                                                      <  2:1818958/93‑1 (MQ=255)
   aaGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGtt                                                                                      <  2:2391616/93‑1 (MQ=255)
     gTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTc                                                                                    >  2:1383873/1‑93 (MQ=255)
      tttttGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTcc                                                                                   <  2:547780/93‑1 (MQ=255)
        tttGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTg                                                                                 <  1:4120060/93‑1 (MQ=255)
        tttGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTg                                                                                 <  2:793857/93‑1 (MQ=255)
        tttGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTg                                                                                 <  1:1600/93‑1 (MQ=255)
         ttGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTgg                                                                                >  1:4136063/1‑93 (MQ=255)
          tGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGa                                                                               <  1:3411093/93‑1 (MQ=255)
            tCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGaaa                                                                             >  2:2132044/1‑93 (MQ=255)
               gTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGaaaaaa                                                                          >  2:2469239/1‑92 (MQ=255)
               gTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAac                                                                          >  1:2541374/1‑93 (MQ=255)
                  ccATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACAcc                                                                       <  1:2646611/93‑1 (MQ=255)
                  ccATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACAcc                                                                       >  1:3459143/1‑93 (MQ=255)
                   cATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACct                                                                      >  1:3739508/1‑93 (MQ=255)
                   cATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACct                                                                      >  1:2982885/1‑93 (MQ=255)
                   cATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACct                                                                      <  2:3335876/93‑1 (MQ=255)
                    aTAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACctg                                                                     >  1:1416579/1‑93 (MQ=255)
                     tAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACctgc                                                                    <  1:2133895/93‑1 (MQ=255)
                     tAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACctgc                                                                    <  2:1486508/93‑1 (MQ=255)
                       aGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACctgctg                                                                  >  2:2808475/1‑93 (MQ=255)
                        gCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCtgctgc                                                                 >  1:3894377/1‑93 (MQ=255)
                           aTGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCatt                                                              >  2:1023375/1‑93 (MQ=255)
                           aTGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCatt                                                              >  2:3141458/1‑93 (MQ=255)
                            tGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCatta                                                             >  2:416681/1‑93 (MQ=255)
                            tGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCatta                                                             <  1:2238606/93‑1 (MQ=255)
                             gTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCattat                                                            <  1:4280372/93‑1 (MQ=255)
                              tCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCattatt                                                           <  1:3029170/93‑1 (MQ=255)
                                cAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtc                                                         >  1:3588409/1‑93 (MQ=255)
                                 aGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcc                                                        >  2:1222972/1‑93 (MQ=255)
                                 aGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcc                                                        <  1:2378856/93‑1 (MQ=255)
                                 aGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcc                                                        >  2:2825958/1‑93 (MQ=255)
                                  gACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcct                                                       >  1:1230811/1‑93 (MQ=255)
                                  gACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcct                                                       >  1:369694/1‑93 (MQ=255)
                                   aCAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcctc                                                      <  2:3465932/93‑1 (MQ=255)
                                   aCAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcctc                                                      <  2:2108230/93‑1 (MQ=255)
                                    cAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcctcc                                                     <  2:1552588/93‑1 (MQ=255)
                                    cAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcctcc                                                     <  1:2770939/93‑1 (MQ=255)
                                     aaaGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCg                                                    >  1:4233217/1‑93 (MQ=255)
                                     aaaGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCg                                                    >  2:28831/1‑93 (MQ=255)
                                       aGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCggg                                                  >  1:3597052/1‑93 (MQ=255)
                                        gATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGa                                                 <  1:2034056/93‑1 (MQ=255)
                                        gATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGa                                                 <  2:4303504/93‑1 (MQ=255)
                                         aTTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGaa                                                >  2:4620014/1‑93 (MQ=255)
                                          ttttCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGaaa                                               >  1:1852471/1‑93 (MQ=255)
                                          ttttCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGaaa                                               >  1:532692/1‑93 (MQ=255)
                                          ttttCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGaaa                                               >  2:1537264/1‑93 (MQ=255)
                                           tttCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAAt                                              <  2:3567174/93‑1 (MQ=255)
                                                gTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAg                                         >  1:2694899/1‑93 (MQ=255)
                                                 tttCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGa                                        >  2:4052158/1‑93 (MQ=255)
                                                     tACTTTATGATCATAATCAATAACTGAATTAAGAACCCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaata                                    <  1:3223110/93‑1 (MQ=255)
                                                     tACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaata                                    >  1:4597766/1‑93 (MQ=255)
                                                      aCTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaataa                                   <  1:2811668/93‑1 (MQ=255)
                                                      aCTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaataa                                   <  2:2580037/93‑1 (MQ=255)
                                                      aCTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaataa                                   >  2:2710449/1‑93 (MQ=255)
                                                       cTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAGCACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaataat                                  <  2:2919399/93‑1 (MQ=255)
                                                       cTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaataat                                  >  2:3663315/1‑93 (MQ=255)
                                                       cTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaataat                                  >  1:2480724/1‑93 (MQ=255)
                                                       cTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAaataat                                  <  1:1392728/93‑1 (MQ=255)
                                                        tttATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAAtt                                 >  2:3100225/1‑93 (MQ=255)
                                                        tttATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAAtt                                 <  2:46099/93‑1 (MQ=255)
                                                         ttATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTa                                <  2:3641187/93‑1 (MQ=255)
                                                         ttATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTCCCTCCGGGAAATAGCAGAAATAATTa                                <  2:2315200/93‑1 (MQ=255)
                                                          tATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTAc                               >  2:4686394/1‑93 (MQ=255)
                                                           aTGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTAcc                              <  1:1382432/93‑1 (MQ=255)
                                                             gATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCtt                            >  1:976871/1‑93 (MQ=255)
                                                             gATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCtt                            >  1:4414460/1‑93 (MQ=255)
                                                              acattAATCAATAACTGAATTAAGAACCCAAGTTTCCTGGAAAACCCCCGCTTGAATTATTCCCTCGGGGAAATAGCAGAAATAATTACCTTa                           <  1:738380/89‑1 (MQ=255)
                                                              aTCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTa                           <  2:4277996/93‑1 (MQ=255)
                                                                cATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATg                         <  1:4353360/93‑1 (MQ=255)
                                                                 aTAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGt                        <  2:1390817/93‑1 (MQ=255)
                                                                  tAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTa                       <  1:1888044/93‑1 (MQ=255)
                                                                   aaTCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTaa                      <  2:3233668/93‑1 (MQ=255)
                                                                   aaTCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTaa                      >  2:4474657/1‑93 (MQ=255)
                                                                   aaTCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTaa                      >  1:3925318/1‑93 (MQ=255)
                                                                   aaTCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTaa                      >  2:3985563/1‑93 (MQ=255)
                                                                    aTCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAAt                     >  1:166663/1‑93 (MQ=255)
                                                                     tCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATc                    <  2:3872886/93‑1 (MQ=255)
                                                                        aTAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTAt                 >  1:3643538/1‑93 (MQ=255)
                                                                          aaCTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCg               <  2:3533445/93‑1 (MQ=255)
                                                                            cTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTa             <  2:119705/93‑1 (MQ=255)
                                                                            cTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTa             >  1:2190890/1‑93 (MQ=255)
                                                                             tGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTaa            <  2:2288909/93‑1 (MQ=255)
                                                                              gAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAAt           <  2:1732776/93‑1 (MQ=255)
                                                                               aaTTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATa          <  1:709471/93‑1 (MQ=255)
                                                                               aaTTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATa          >  1:813854/1‑93 (MQ=255)
                                                                               aaTTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATa          >  1:4047596/1‑93 (MQ=255)
                                                                                aTTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATAc         >  2:3263266/1‑93 (MQ=255)
                                                                                 ttAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACt        >  1:1533743/1‑93 (MQ=255)
                                                                                 ttAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACt        >  1:3297174/1‑93 (MQ=255)
                                                                                 ttAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACt        >  2:2224549/1‑93 (MQ=255)
                                                                                 ttAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACt        >  2:2797740/1‑93 (MQ=255)
                                                                                  tAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACtt       <  2:2725470/93‑1 (MQ=255)
                                                                                   aaGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTc      <  1:4673332/93‑1 (MQ=255)
                                                                                     gAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGa    >  1:1707249/1‑93 (MQ=255)
                                                                                       aaCCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGAtt  >  2:4456816/1‑93 (MQ=255)
                                                                                       aaCCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGAtt  >  2:619323/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATT  >  NC_000913/3800567‑3800746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTA  >  NC_000913/3800564‑3800750
                                                                                             |                                                                                             
AATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACC                                                                                               <  SRR4302535.4685653/94‑1 (MQ=60)
 ATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCC                                                                                              <  SRR4302535.4219556/94‑1 (MQ=60)
    GAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGT                                                                                           <  SRR4302535.1021496/94‑1 (MQ=60)
    GAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGT                                                                                           >  SRR4302535.1397057/1‑94 (MQ=60)
       AGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCAAGTTTC                                                                                        >  SRR4302535.1477731/1‑94 (MQ=60)
         TTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCT                                                                                      <  SRR4302535.303836/94‑1 (MQ=60)
         TTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATAAATAACTGAATTAAGAAACCCAGTTTCCT                                                                                      <  SRR4302535.1241867/94‑1 (MQ=60)
           TTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGG                                                                                    >  SRR4302535.4164383/1‑94 (MQ=60)
           TTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGG                                                                                    <  SRR4302535.4148255/94‑1 (MQ=60)
           TTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGG                                                                                    <  SRR4302535.1650099/94‑1 (MQ=60)
           TTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGG                                                                                    <  SRR4302535.1618/94‑1 (MQ=60)
             TGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAA                                                                                  <  SRR4302535.3434824/94‑1 (MQ=60)
                 AGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAAC                                                                              >  SRR4302535.2559679/1‑94 (MQ=60)
                  GTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACA                                                                             <  SRR4302535.4508956/94‑1 (MQ=60)
                    TCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACC                                                                           >  SRR4302535.3483192/1‑94 (MQ=60)
                     CCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCT                                                                          >  SRR4302535.3765389/1‑94 (MQ=60)
                     CCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCT                                                                          <  SRR4302535.2665566/94‑1 (MQ=60)
                     CCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCT                                                                          >  SRR4302535.3003807/1‑94 (MQ=60)
                      CATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTG                                                                         >  SRR4302535.1426693/1‑94 (MQ=60)
                       ATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCGAGTTTCCTGGAAAAACACCTGC                                                                        >  SRR4302535.98079/1‑94 (MQ=60)
                       ATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGC                                                                        >  SRR4302535.4123038/1‑94 (MQ=60)
                        TAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCT                                                                       <  SRR4302535.1897609/94‑1 (MQ=60)
                        TAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCT                                                                       >  SRR4302535.1819877/1‑94 (MQ=60)
                        TAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCT                                                                       <  SRR4302535.2149273/94‑1 (MQ=60)
                          AGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGC                                                                     >  SRR4302535.2715893/1‑94 (MQ=60)
                          AGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGC                                                                     >  SRR4302535.3921071/1‑94 (MQ=60)
                            CGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCAT                                                                   >  SRR4302535.3974934/1‑94 (MQ=60)
                              ATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTA                                                                 >  SRR4302535.3921454/1‑94 (MQ=60)
                              ATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTA                                                                 >  SRR4302535.2022844/1‑94 (MQ=60)
                               TGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTAT                                                                <  SRR4302535.2254532/94‑1 (MQ=60)
                                GTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATT                                                               <  SRR4302535.4309659/94‑1 (MQ=60)
                                 TCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTT                                                              <  SRR4302535.3050373/94‑1 (MQ=60)
                                  CCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTC                                                             >  SRR4302535.3613301/1‑94 (MQ=60)
                                    AGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCT                                                           >  SRR4302535.372247/1‑94 (MQ=60)
                                    AGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCT                                                           >  SRR4302535.1239612/1‑94 (MQ=60)
                                    AGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCT                                                           <  SRR4302535.2395941/94‑1 (MQ=60)
                                      ACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCC                                                         >  SRR4302535.2191595/1‑94 (MQ=60)
                                      ACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCC                                                         >  SRR4302535.3787826/1‑94 (MQ=60)
                                       CAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCG                                                        >  SRR4302535.4262185/1‑94 (MQ=60)
                                       CAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCG                                                        <  SRR4302535.2790671/94‑1 (MQ=60)
                                       CAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCG                                                        >  SRR4302535.307017/1‑94 (MQ=60)
                                         AAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGG                                                      >  SRR4302535.3622013/1‑94 (MQ=60)
                                          AGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGA                                                     <  SRR4302535.3683044/94‑1 (MQ=60)
                                          AGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGA                                                     >  SRR4302535.2252991/1‑94 (MQ=60)
                                           GATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAA                                                    >  SRR4302535.4295492/1‑94 (MQ=60)
                                           GATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAA                                                    <  SRR4302535.2048712/94‑1 (MQ=60)
                                            ATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAA                                                   >  SRR4302535.536421/1‑94 (MQ=60)
                                            ATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAA                                                   >  SRR4302535.1865816/1‑94 (MQ=60)
                                              TTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATA                                                 >  SRR4302535.3748141/1‑94 (MQ=60)
                                               TTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAG                                                >  SRR4302535.1412950/1‑94 (MQ=60)
                                                  TGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAG                                             >  SRR4302535.2714162/1‑94 (MQ=60)
                                                  TGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAG                                             >  SRR4302535.4681861/1‑94 (MQ=60)
                                                  TGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAG                                             <  SRR4302535.3649665/94‑1 (MQ=60)
                                                     TTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAA                                          >  SRR4302535.2459851/1‑94 (MQ=60)
                                                       CTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATA                                        >  SRR4302535.725613/1‑94 (MQ=60)
                                                       CTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATA                                        >  SRR4302535.4629043/1‑94 (MQ=60)
                                                       CTACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATA                                        <  SRR4302535.4349860/94‑1 (MQ=60)
                                                        TACTTTATGATCATAATCAATAACTGAATTAAGAACCCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAA                                       <  SRR4302535.3245453/94‑1 (MQ=60)
                                                        TACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAA                                       >  SRR4302535.2761525/1‑94 (MQ=60)
                                                         ACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAAT                                      >  SRR4302535.2546962/1‑94 (MQ=60)
                                                         ACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAAT                                      <  SRR4302535.3346045/94‑1 (MQ=60)
                                                         ACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAAT                                      <  SRR4302535.2831653/94‑1 (MQ=60)
                                                         ACTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAAT                                      >  SRR4302535.2498611/1‑94 (MQ=60)
                                                          CTTTATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATT                                     <  SRR4302535.1402672/94‑1 (MQ=60)
                                                             TATGATCATAATCAATAACTGAATTAAGAATCCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACC                                  <  SRR4302535.689609/94‑1 (MQ=60)
                                                              ATGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCT                                 <  SRR4302535.1392293/94‑1 (MQ=60)
                                                               TGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTT                                >  SRR4302535.983806/1‑94 (MQ=60)
                                                               TGATCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTT                                >  SRR4302535.4444673/1‑94 (MQ=60)
                                                                 acatTAATCAATAACTGAATTAAGAACCCAAGTTTCCTGGAAAACCCCCGCTTGAATTATTCCCTCGGGGAAATAGCAGAAATAATTACCTTAT                              <  SRR4302535.743573/90‑1 (MQ=25)
                                                                  TCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATG                             <  SRR4302535.502078/94‑1 (MQ=60)
                                                                  TCATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATG                             <  SRR4302535.1596838/94‑1 (MQ=60)
                                                                   CATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGT                            <  SRR4302535.4383155/94‑1 (MQ=60)
                                                                   CATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGT                            >  SRR4302535.2320935/1‑94 (MQ=60)
                                                                    ATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTA                           >  SRR4302535.4430603/1‑94 (MQ=60)
                                                                    ATAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTA                           <  SRR4302535.994932/94‑1 (MQ=60)
                                                                     TAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAA                          >  SRR4302535.3952141/1‑94 (MQ=60)
                                                                     TAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAA                          <  SRR4302535.1901644/94‑1 (MQ=60)
                                                                     TAATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAA                          >  SRR4302535.2852746/1‑94 (MQ=60)
                                                                      AATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAAT                         >  SRR4302535.167823/1‑94 (MQ=60)
                                                                       ATCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATC                        <  SRR4302535.3561649/94‑1 (MQ=60)
                                                                        TCAATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCT                       >  SRR4302535.767774/1‑94 (MQ=60)
                                                                          AATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTAT                     <  SRR4302535.2655734/94‑1 (MQ=60)
                                                                          AATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTAT                     >  SRR4302535.3668771/1‑94 (MQ=60)
                                                                          AATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTAT                     >  SRR4302535.280693/1‑94 (MQ=60)
                                                                           ATAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATC                    <  SRR4302535.4380225/94‑1 (MQ=60)
                                                                            TAACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCG                   >  SRR4302535.1689744/1‑94 (MQ=60)
                                                                              ACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTA                 >  SRR4302535.4179277/1‑94 (MQ=60)
                                                                              ACTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTA                 >  SRR4302535.2206486/1‑94 (MQ=60)
                                                                               CTGAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAA                <  SRR4302535.3459940/94‑1 (MQ=60)
                                                                                 tgATTAAGAACCCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGTAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATA              <  SRR4302535.2130431/92‑1 (MQ=60)
                                                                                 GAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATA              >  SRR4302535.2692792/1‑94 (MQ=60)
                                                                                 GAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATA              >  SRR4302535.4075302/1‑94 (MQ=60)
                                                                                 GAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATA              <  SRR4302535.4157137/94‑1 (MQ=60)
                                                                                 GAATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATA              >  SRR4302535.819562/1‑94 (MQ=60)
                                                                                  AATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATAC             <  SRR4302535.714450/94‑1 (MQ=60)
                                                                                   ATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACT            >  SRR4302535.3320097/1‑94 (MQ=60)
                                                                                   ATTAAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACT            >  SRR4302535.1544793/1‑94 (MQ=60)
                                                                                      AAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCG         <  SRR4302535.4704888/94‑1 (MQ=60)
                                                                                      AAGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCG         <  SRR4302535.3617364/94‑1 (MQ=60)
                                                                                       AGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGA        >  SRR4302535.3266214/1‑94 (MQ=60)
                                                                                       AGAAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGA        >  SRR4302535.1719593/1‑94 (MQ=60)
                                                                                         AAACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATT      <  SRR4302535.1062828/94‑1 (MQ=60)
                                                                                          AACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTAGGTAATCTATCGTAATACTTCGATTG     >  SRR4302535.4559719/1‑94 (MQ=60)
                                                                                           ACCCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGG    <  SRR4302535.4295791/94‑1 (MQ=60)
                                                                                             CCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTA  >  SRR4302535.4212994/1‑94 (MQ=60)
                                                                                             CCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTA  >  SRR4302535.607802/1‑94 (MQ=60)
                                                                                             |                                                                                             
AATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTA  >  NC_000913/3800564‑3800750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑