Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,812,423 T→C R101R (CGT→CGC)  b3641 → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,812,4230TC100.0% 731.6 / NA 230R101R (CGT→CGC) b3641putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (109/121);  total (109/121)

Only 100 of 239 total aligned reads displayed.

TACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCG  >  NC_000913/3812333‑3812512
                                                                                          |                                                                                         
tACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCaa                                                                                         >  1:3627647/1‑93 (MQ=255)
tACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCaa                                                                                         <  2:151936/93‑1 (MQ=255)
 aCTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAAt                                                                                        >  2:3472689/1‑93 (MQ=255)
 aCTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGCTTTGGTGAGCGCAAt                                                                                        >  2:3682294/1‑93 (MQ=255)
  cTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATc                                                                                       <  2:1176686/93‑1 (MQ=255)
   tCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATcc                                                                                      <  1:3843805/93‑1 (MQ=255)
      cATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTgg                                                                                   <  2:2667300/93‑1 (MQ=255)
       aTCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGc                                                                                  >  2:3549025/1‑93 (MQ=255)
         cAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCt                                                                                <  1:2585468/93‑1 (MQ=255)
           aCCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGa                                                                              >  1:4049705/1‑93 (MQ=255)
           aCCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGa                                                                              >  1:2276939/1‑93 (MQ=255)
            ccTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATGCTGGCCTGAc                                                                             >  1:3417615/1‑93 (MQ=255)
             cTGATTCTGAAACATGAGAAAGCCACCCCAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTTGCCCGAcc                                                                            >  2:2293296/1‑93 (MQ=255)
              tGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGAccc                                                                           >  1:4577571/1‑93 (MQ=255)
              tGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGAccc                                                                           <  2:3645006/93‑1 (MQ=255)
              tGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGAccc                                                                           <  1:999346/93‑1 (MQ=255)
                 ttCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCa                                                                        >  2:180272/1‑93 (MQ=255)
                   cTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATc                                                                      <  1:2527699/93‑1 (MQ=255)
                    tGAAAGAGGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATcc                                                                     <  1:1032425/93‑1 (MQ=255)
                     gAAAGATGAGAAGGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCt                                                                    >  1:3200530/1‑93 (MQ=255)
                     gAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCt                                                                    <  2:1030008/93‑1 (MQ=255)
                     gAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCt                                                                    >  2:1316651/1‑93 (MQ=255)
                      aaaGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTc                                                                   <  2:4628979/93‑1 (MQ=255)
                       aaGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTcc                                                                  >  1:2311983/1‑92 (MQ=255)
                       aaGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCa                                                                  >  1:4097330/1‑93 (MQ=255)
                       aaGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCa                                                                  <  1:399269/93‑1 (MQ=255)
                        aGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCAc                                                                 <  1:2988059/93‑1 (MQ=255)
                        aGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCAc                                                                 <  1:117244/93‑1 (MQ=255)
                        aGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGGTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCAc                                                                 >  2:1634447/1‑93 (MQ=255)
                         gATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACt                                                                <  2:1249606/93‑1 (MQ=255)
                         gATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACt                                                                <  2:4390794/93‑1 (MQ=255)
                         gATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACt                                                                >  1:1430243/1‑93 (MQ=255)
                          aTGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTg                                                               <  1:1762910/93‑1 (MQ=255)
                          aTGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTg                                                               <  1:1740514/93‑1 (MQ=255)
                           tGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTgg                                                              >  1:3017161/1‑93 (MQ=255)
                           ggagaAAGACGCCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTGGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTgg                                                              <  2:1952255/92‑1 (MQ=255)
                             agaAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTc                                                            <  1:1978467/93‑1 (MQ=255)
                              gaAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCa                                                           <  2:321210/93‑1 (MQ=255)
                               aaaGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCAt                                                          <  2:2712234/93‑1 (MQ=255)
                               aaaGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCAt                                                          <  1:2660433/93‑1 (MQ=255)
                                aaGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATg                                                         <  2:1346260/93‑1 (MQ=255)
                                 aGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATgc                                                        >  2:4590134/1‑93 (MQ=255)
                                 aGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATgc                                                        <  1:2649350/93‑1 (MQ=255)
                                  gACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATgcg                                                       <  2:4048495/93‑1 (MQ=255)
                                      ccacAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTaa                                                   >  1:1536379/1‑93 (MQ=255)
                                      ccacAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTaa                                                   >  2:1466538/1‑93 (MQ=255)
                                      ccacAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTaa                                                   >  2:4128147/1‑93 (MQ=255)
                                       cacaGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAAt                                                  >  2:935217/1‑93 (MQ=255)
                                       cacaGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAAt                                                  >  1:573428/1‑93 (MQ=255)
                                        acaGCGCGCCTGCGTCTGATTGTGCTGCTGCTTCTCTGTTTTGCTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATg                                                 <  2:3324191/93‑1 (MQ=255)
                                          aGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGtt                                               >  2:1662608/1‑93 (MQ=255)
                                           gcgcgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGttt                                              >  1:867180/1‑93 (MQ=255)
                                            cgcgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTg                                             >  1:4639496/1‑93 (MQ=255)
                                             gcgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGa                                            >  1:1226085/1‑93 (MQ=255)
                                             gcgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTAGa                                            <  1:3319452/93‑1 (MQ=255)
                                              cgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGaa                                           <  2:2361738/93‑1 (MQ=255)
                                              cgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGaa                                           >  2:3926921/1‑93 (MQ=255)
                                               gcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAc                                          <  1:3460190/93‑1 (MQ=255)
                                               gcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAc                                          >  1:1919633/1‑93 (MQ=255)
                                               gcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAc                                          <  1:1846226/93‑1 (MQ=255)
                                               gcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAc                                          <  1:3605355/93‑1 (MQ=255)
                                                ccTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACa                                         <  1:3136989/93‑1 (MQ=255)
                                                 cTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAg                                        <  1:4323445/93‑1 (MQ=255)
                                                  tGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAgg                                       >  1:2078259/1‑93 (MQ=255)
                                                  tGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAgg                                       >  1:4240398/1‑93 (MQ=255)
                                                    cGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGAt                                     <  1:711585/93‑1 (MQ=255)
                                                    cGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGAt                                     <  2:1540830/93‑1 (MQ=255)
                                                    cGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGAt                                     <  1:2944853/93‑1 (MQ=255)
                                                      tCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTTATGTTTGAACAGGATCg                                   >  1:4204139/1‑93 (MQ=255)
                                                      tCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCg                                   >  2:2219998/1‑93 (MQ=255)
                                                      tCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCg                                   >  1:377282/1‑93 (MQ=255)
                                                        tGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGcc                                 <  2:3024917/93‑1 (MQ=255)
                                                         taTTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCt                                <  2:3170125/92‑1 (MQ=255)
                                                            tgtgTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCa                             >  1:2437192/1‑93 (MQ=255)
                                                             gtgtTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCaa                            >  2:67940/1‑93 (MQ=255)
                                                               tttGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAgg                          <  2:2871563/92‑1 (MQ=255)
                                                               gtTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAgg                          <  2:1318347/93‑1 (MQ=255)
                                                               gtTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAgg                          <  1:1631988/93‑1 (MQ=255)
                                                               gtTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAgg                          <  2:2006346/93‑1 (MQ=255)
                                                                    tgctTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCa                     <  2:4252728/93‑1 (MQ=255)
                                                                     gctTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCAt                    >  1:2569454/1‑93 (MQ=255)
                                                                     gctTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGCGCGCac                    >  2:4480870/1‑92 (MQ=255)
                                                                       ttCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCa                  >  1:2426357/1‑93 (MQ=255)
                                                                        tctcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCaa                 <  2:3914289/93‑1 (MQ=255)
                                                                        tctcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCaa                 <  1:2171414/93‑1 (MQ=255)
                                                                         ttcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCACTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAAc                <  2:3752677/92‑1 (MQ=255)
                                                                         ctcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAAc                <  1:1067804/93‑1 (MQ=255)
                                                                         ctcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAAc                <  2:534918/93‑1 (MQ=255)
                                                                             gTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGc            <  2:2588104/93‑1 (MQ=255)
                                                                             gTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGc            >  2:1087450/1‑93 (MQ=255)
                                                                               tttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTg          <  1:375907/93‑1 (MQ=255)
                                                                                ttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGt         <  1:670891/93‑1 (MQ=255)
                                                                                ttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGt         >  1:3682721/1‑93 (MQ=255)
                                                                                ttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGt         <  1:2403060/93‑1 (MQ=255)
                                                                                 tGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGtt        >  2:2049868/1‑93 (MQ=255)
                                                                                 tGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGtt        <  1:841957/93‑1 (MQ=255)
                                                                                   gTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCg      >  1:3835092/1‑93 (MQ=255)
                                                                                      aGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGc   <  1:212958/93‑1 (MQ=255)
                                                                                      aGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGc   >  1:1686790/1‑93 (MQ=255)
                                                                                       gcgcAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCg  <  2:2442217/93‑1 (MQ=255)
                                                                                          |                                                                                         
TACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCG  >  NC_000913/3812333‑3812512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCGTATT  >  NC_000913/3812330‑3812516
                                                                                             |                                                                                             
GATTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGC                                                                                               >  SRR4302535.2599504/1‑94 (MQ=60)
GATTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGC                                                                                               >  SRR4302535.868560/1‑94 (MQ=60)
GATTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGC                                                                                               >  SRR4302535.463078/1‑94 (MQ=60)
 ATTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCA                                                                                              >  SRR4302535.1680489/1‑94 (MQ=60)
  TTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAA                                                                                             >  SRR4302535.3652791/1‑94 (MQ=60)
    ACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGGGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATC                                                                                           >  SRR4302535.2539658/1‑94 (MQ=60)
     CTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCC                                                                                          <  SRR4302535.2050860/94‑1 (MQ=60)
      TCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCT                                                                                         <  SRR4302535.3870280/94‑1 (MQ=60)
            CAACCTGATTCTGAAAGATGAGAAAGAGACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTG                                                                                   <  SRR4302535.2837447/94‑1 (MQ=60)
            CAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTG                                                                                   <  SRR4302535.2604037/94‑1 (MQ=60)
             AACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGA                                                                                  >  SRR4302535.4077431/1‑94 (MQ=60)
             AACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGA                                                                                  >  SRR4302535.2293197/1‑94 (MQ=60)
             AACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGA                                                                                  <  SRR4302535.172087/94‑1 (MQ=60)
              ACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCACTCCTGGCCTGAC                                                                                 >  SRR4302535.916646/1‑94 (MQ=60)
              ACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATGCTGGCCTGAC                                                                                 >  SRR4302535.3441389/1‑94 (MQ=60)
              ACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGAC                                                                                 <  SRR4302535.912626/94‑1 (MQ=60)
                CTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCC                                                                               >  SRR4302535.4608756/1‑94 (MQ=60)
                 TGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCG                                                                              <  SRR4302535.1006433/94‑1 (MQ=60)
                 TGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCG                                                                              >  SRR4302535.311751/1‑94 (MQ=60)
                    TTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCAT                                                                           >  SRR4302535.1664283/1‑94 (MQ=60)
                    TTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCAT                                                                           <  SRR4302535.4035957/94‑1 (MQ=60)
                      CTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCC                                                                         <  SRR4302535.2545908/94‑1 (MQ=60)
                      CTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCC                                                                         >  SRR4302535.2234941/1‑94 (MQ=60)
                       TGAAAGATGAGAAGGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCT                                                                        >  SRR4302535.3222687/1‑94 (MQ=60)
                       TGAAAGAGGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCT                                                                        <  SRR4302535.1039761/94‑1 (MQ=60)
                         AAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCC                                                                      >  SRR4302535.2328536/1‑94 (MQ=60)
                         AAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCA                                                                      >  SRR4302535.4015095/1‑94 (MQ=60)
                         AAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCA                                                                      >  SRR4302535.4125375/1‑94 (MQ=60)
                          AAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCAC                                                                     <  SRR4302535.402066/94‑1 (MQ=60)
                           AGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACT                                                                    <  SRR4302535.118068/94‑1 (MQ=60)
                           AGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACT                                                                    <  SRR4302535.66769/94‑1 (MQ=60)
                           AGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACT                                                                    <  SRR4302535.3009013/94‑1 (MQ=60)
                           AGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACT                                                                    <  SRR4302535.3539137/94‑1 (MQ=60)
                           AGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACT                                                                    >  SRR4302535.1440447/1‑94 (MQ=60)
                           AGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACT                                                                    >  SRR4302535.2387784/1‑94 (MQ=60)
                            GATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTG                                                                   >  SRR4302535.112068/1‑94 (MQ=60)
                             ATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGG                                                                  <  SRR4302535.1753093/94‑1 (MQ=60)
                             ATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGG                                                                  >  SRR4302535.3038298/1‑94 (MQ=60)
                             ATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGG                                                                  <  SRR4302535.1775679/94‑1 (MQ=60)
                              TGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGT                                                                 >  SRR4302535.3785074/1‑94 (MQ=60)
                              AGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGT                                                                 <  SRR4302535.4311718/94‑1 (MQ=60)
                               GAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTC                                                                >  SRR4302535.4652262/1‑94 (MQ=60)
                                AGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCA                                                               <  SRR4302535.1992716/94‑1 (MQ=60)
                                  AAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATG                                                             >  SRR4302535.2992435/1‑94 (MQ=60)
                                  AAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATG                                                             <  SRR4302535.2679491/94‑1 (MQ=60)
                                   AAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGC                                                            >  SRR4302535.2237/1‑94 (MQ=60)
                                   AAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGC                                                            >  SRR4302535.3163862/1‑94 (MQ=60)
                                   AAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGC                                                            <  SRR4302535.335510/94‑1 (MQ=60)
                                    AGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCG                                                           <  SRR4302535.2668323/94‑1 (MQ=60)
                                     GACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGC                                                          >  SRR4302535.2678543/1‑94 (MQ=60)
                                      ACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCT                                                         <  SRR4302535.3868455/94‑1 (MQ=60)
                                       CACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTA                                                        >  SRR4302535.3091966/1‑94 (MQ=60)
                                       CACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCCTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTA                                                        >  SRR4302535.2565085/1‑94 (MQ=60)
                                        ACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAA                                                       >  SRR4302535.1547447/1‑94 (MQ=60)
                                         CCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAAT                                                      >  SRR4302535.577452/1‑94 (MQ=60)
                                          CACAGCGCGCCTGCGTCTGATTGTTTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATG                                                     >  SRR4302535.3657854/1‑94 (MQ=60)
                                          CACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATG                                                     >  SRR4302535.148342/1‑94 (MQ=60)
                                          CACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATG                                                     >  SRR4302535.4205515/1‑94 (MQ=60)
                                          CACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATG                                                     >  SRR4302535.3029550/1‑94 (MQ=60)
                                            CAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTT                                                   >  SRR4302535.3226232/1‑94 (MQ=60)
                                             AGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTT                                                  >  SRR4302535.873315/1‑94 (MQ=60)
                                             AGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTT                                                  <  SRR4302535.4072653/94‑1 (MQ=60)
                                              GCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTG                                                 <  SRR4302535.1719576/94‑1 (MQ=60)
                                              GCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTG                                                 >  SRR4302535.4670956/1‑94 (MQ=60)
                                              GCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTG                                                 <  SRR4302535.3814899/94‑1 (MQ=60)
                                              GCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTG                                                 <  SRR4302535.3446526/94‑1 (MQ=60)
                                               CGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGA                                                >  SRR4302535.751651/1‑94 (MQ=60)
                                               CGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGA                                                >  SRR4302535.1234855/1‑94 (MQ=60)
                                                GCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTAGAA                                               <  SRR4302535.3342547/94‑1 (MQ=60)
                                                 CGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAC                                              <  SRR4302535.32263/94‑1 (MQ=60)
                                                 CGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAC                                              >  SRR4302535.1208161/1‑94 (MQ=60)
                                                 CGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAC                                              >  SRR4302535.1933470/1‑94 (MQ=60)
                                                 CGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAC                                              >  SRR4302535.3843837/1‑94 (MQ=60)
                                                  GCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACA                                             <  SRR4302535.4522064/94‑1 (MQ=60)
                                                  GCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACA                                             <  SRR4302535.4406909/94‑1 (MQ=60)
                                                  GCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACA                                             <  SRR4302535.122190/94‑1 (MQ=60)
                                                  GCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACA                                             <  SRR4302535.3484247/94‑1 (MQ=60)
                                                  GCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACA                                             <  SRR4302535.1859533/94‑1 (MQ=60)
                                                  GCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACA                                             <  SRR4302535.3630366/94‑1 (MQ=60)
                                                   CCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAG                                            <  SRR4302535.3158820/94‑1 (MQ=60)
                                                    CTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGG                                           <  SRR4302535.4353020/94‑1 (MQ=60)
                                                    CTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGG                                           >  SRR4302535.2093231/1‑94 (MQ=60)
                                                    CTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGG                                           >  SRR4302535.4269421/1‑94 (MQ=60)
                                                    CTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGG                                           >  SRR4302535.3639350/1‑94 (MQ=60)
                                                     TGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGA                                          >  SRR4302535.2036476/1‑94 (MQ=60)
                                                     TGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGA                                          >  SRR4302535.376839/1‑94 (MQ=60)
                                                      GCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGAT                                         <  SRR4302535.79495/94‑1 (MQ=60)
                                                      GCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGAT                                         <  SRR4302535.2428226/94‑1 (MQ=60)
                                                       CGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATC                                        <  SRR4302535.716581/94‑1 (MQ=60)
                                                       CGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATC                                        <  SRR4302535.1764999/94‑1 (MQ=60)
                                                       CGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATC                                        <  SRR4302535.2606262/94‑1 (MQ=60)
                                                       CGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATC                                        <  SRR4302535.2965551/94‑1 (MQ=60)
                                                        GTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTTATGTTTGAACAGGATCG                                       >  SRR4302535.4232913/1‑94 (MQ=60)
                                                        GTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCG                                       >  SRR4302535.559257/1‑94 (MQ=60)
                                                        GTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCG                                       >  SRR4302535.379901/1‑94 (MQ=60)
                                                        GTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCG                                       >  SRR4302535.2445812/1‑94 (MQ=60)
                                                          CTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCC                                     <  SRR4302535.3677444/94‑1 (MQ=60)
                                                          CTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATAGCC                                     >  SRR4302535.2361619/1‑94 (MQ=60)
                                                           TGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCT                                    <  SRR4302535.211511/94‑1 (MQ=60)
                                                           TGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCT                                    >  SRR4302535.2629963/1‑94 (MQ=60)
                                                            GATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTG                                   <  SRR4302535.2600246/94‑1 (MQ=60)
                                                             ATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGC                                  >  SRR4302535.2921644/1‑94 (MQ=60)
                                                              TTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCA                                 <  SRR4302535.1609052/94‑1 (MQ=60)
                                                              TTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCA                                 >  SRR4302535.2454749/1‑94 (MQ=60)
                                                              TTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCA                                 >  SRR4302535.4695682/1‑94 (MQ=60)
                                                               TGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAA                                >  SRR4302535.1915320/1‑94 (MQ=60)
                                                                GTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAG                               >  SRR4302535.1246956/1‑94 (MQ=60)
                                                                 TGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGG                              <  SRR4302535.1788599/94‑1 (MQ=60)
                                                                  tgTGCTGCTTCGCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGG                             <  SRR4302535.4081207/92‑1 (MQ=60)
                                                                  GTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGG                             <  SRR4302535.1643780/94‑1 (MQ=60)
                                                                      CTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGCCCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCA                         >  SRR4302535.4249180/1‑94 (MQ=60)
                                                                      CTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCA                         >  SRR4302535.422186/1‑94 (MQ=60)
                                                                       TGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCAT                        >  SRR4302535.2301104/1‑94 (MQ=60)
                                                                       TGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCAT                        >  SRR4302535.2587933/1‑94 (MQ=60)
                                                                       TGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCAT                        <  SRR4302535.2184688/94‑1 (MQ=60)
                                                                         CTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCA                      >  SRR4302535.2443839/1‑94 (MQ=60)
                                                                           TCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAAC                    <  SRR4302535.2186955/94‑1 (MQ=60)
                                                                            CTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACC                   >  SRR4302535.1636683/1‑94 (MQ=60)
                                                                            CTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACC                   <  SRR4302535.1075399/94‑1 (MQ=60)
                                                                               GGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGC                >  SRR4302535.708341/1‑94 (MQ=60)
                                                                               GGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGC                >  SRR4302535.1865796/1‑94 (MQ=60)
                                                                               GGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGC                <  SRR4302535.3586521/94‑1 (MQ=60)
                                                                                  TTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGT             <  SRR4302535.378513/94‑1 (MQ=60)
                                                                                  TTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGT             >  SRR4302535.3708206/1‑94 (MQ=60)
                                                                                   TTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTT            <  SRR4302535.1052901/94‑1 (MQ=60)
                                                                                   TTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTT            <  SRR4302535.2420346/94‑1 (MQ=60)
                                                                                   TTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTT            <  SRR4302535.675574/94‑1 (MQ=60)
                                                                                    TGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTC           <  SRR4302535.847867/94‑1 (MQ=60)
                                                                                     GGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCG          <  SRR4302535.860641/94‑1 (MQ=60)
                                                                                     GGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCG          >  SRR4302535.3861539/1‑94 (MQ=60)
                                                                                       TGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAG        <  SRR4302535.2503170/94‑1 (MQ=60)
                                                                                       TGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAG        >  SRR4302535.1963994/1‑94 (MQ=60)
                                                                                        GAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGC       >  SRR4302535.1699002/1‑94 (MQ=60)
                                                                                         AGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCG      <  SRR4302535.214420/94‑1 (MQ=60)
                                                                                           CGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCGTA    <  SRR4302535.3251425/94‑1 (MQ=60)
                                                                                           CGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCGTA    >  SRR4302535.1993043/1‑94 (MQ=60)
                                                                                            GCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCGTAT   >  SRR4302535.2936953/1‑94 (MQ=60)
                                                                                             CAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCGTATT  <  SRR4302535.3564105/94‑1 (MQ=60)
                                                                                             |                                                                                             
GATTACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGCGTATT  >  NC_000913/3812330‑3812516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑