Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,822,805	T→C	W222R (TGG→CGG)	spoU →	putative RNA methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,822,805	0	T	C	99.6%	782.9 / ‑6.8	249	W222R (TGG→CGG)	spoU	putative RNA methylase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (134/113); total (135/114)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG  >  NC_000913/3822715‑3822894

   ccGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCTGACTCGCGGGct                                                                                      >  2:1370653/1‑93 (MQ=255)

     ggTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctac                                                                                    <  2:3038663/93‑1 (MQ=255)

      gTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAACCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctact                                                                                   <  1:2514555/93‑1 (MQ=255)

       tGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGctacta                                                                                  >  1:348779/1‑93 (MQ=255)

       tGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGccacta                                                                                  >  1:3901975/1‑93 (MQ=255)

        gCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTAt                                                                                 <  2:345961/93‑1 (MQ=255)

          tGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGc                                                                               >  1:2965126/1‑93 (MQ=255)

           ggCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTAGCGGGCTACTATGCa                                                                              <  2:1662798/93‑1 (MQ=255)

              gAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGc                                                                           >  1:623296/1‑93 (MQ=255)

              gAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGc                                                                           >  1:2830466/1‑93 (MQ=255)

              gAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGc                                                                           <  1:3381234/93‑1 (MQ=255)

              gAAAGTCACAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAATCTGATGCCGACTGGCGGGCTACTATGCAGGc                                                                           <  2:611655/93‑1 (MQ=255)

               aaaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCt                                                                          >  2:3769136/1‑93 (MQ=255)

               aaaGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGAGGGCTACTATGCAGGcg                                                                          >  1:853447/1‑92 (MQ=255)

                 aGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGc                                                                        <  2:1078403/93‑1 (MQ=255)

                  gTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGcc                                                                       >  2:3370693/1‑92 (MQ=255)

                      cAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGt                                                                   <  1:3755380/93‑1 (MQ=255)

                      cAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGt                                                                   <  1:1774109/93‑1 (MQ=255)

                      cAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGt                                                                   <  1:1450747/93‑1 (MQ=255)

                      cAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGt                                                                   <  2:3822594/93‑1 (MQ=255)

                       aaaaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTa                                                                  >  2:3096281/1‑93 (MQ=255)

                       aaaaCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGAAGGGTa                                                                  >  2:2928619/1‑93 (MQ=255)

                          aCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAgg                                                               >  1:1069398/1‑92 (MQ=255)

                          aCGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGt                                                               <  2:1859627/93‑1 (MQ=255)

                           cGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTg                                                              <  1:1197160/93‑1 (MQ=255)

                           cGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTg                                                              <  2:3711385/93‑1 (MQ=255)

                           cGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTg                                                              <  1:2011865/93‑1 (MQ=255)

                            gCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGc                                                             <  1:224248/93‑1 (MQ=255)

                            gCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGGGc                                                             >  1:3165224/1‑93 (MQ=255)

                             cAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGGGcc                                                            >  2:340486/1‑93 (MQ=255)

                              aaaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCa                                                           >  1:983482/1‑93 (MQ=255)

                              aaaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCa                                                           >  2:2909250/1‑93 (MQ=255)

                              aaaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCa                                                           <  1:187341/93‑1 (MQ=255)

                              aaaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCa                                                           >  1:3408517/1‑93 (MQ=255)

                               aaGGCCTGCCTTATCTCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCCACTATGCAGGCTGGAGGGCAAGTGCCAt                                                          >  2:120118/1‑93 (MQ=255)

                               aaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAt                                                          >  1:3308770/1‑93 (MQ=255)

                               aaGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGGCAt                                                          >  2:2008965/1‑93 (MQ=255)

                                aGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATg                                                         >  2:1784280/1‑93 (MQ=255)

                                 ggCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATTCAGGCTGCAGGGTAAGTGCCATGa                                                        >  2:2734327/1‑93 (MQ=255)

                                  gcctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGaa                                                       <  2:3294604/93‑1 (MQ=255)

                                  gcctgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGaa                                                       >  1:3328910/1‑93 (MQ=255)

                                    ctgactTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAg                                                     <  2:1012142/93‑1 (MQ=255)

                                     tgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGGAGGGTAAGTGCCATGAAAgg                                                    >  1:1279822/1‑93 (MQ=255)

                                     tgcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAgg                                                    >  2:3199163/1‑93 (MQ=255)

                                      gcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGt                                                   >  2:3499550/1‑93 (MQ=255)

                                      gcctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGt                                                   >  2:3648849/1‑93 (MQ=255)

                                       cctTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTc                                                  <  1:3243866/93‑1 (MQ=255)

                                         ttATCCCCAGGTCAATCAGCAAGGTGAGATTGTATCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTATGTGCCATTAATGGTctc                                                >  1:4682001/1‑91 (MQ=255)

                                         ttATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGc                                                >  2:2384433/1‑93 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGcc                                               <  2:4036988/93‑1 (MQ=255)

                                          tATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTTGcc                                               >  1:2437368/1‑93 (MQ=255)

                                           aTCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCt                                              <  2:1445955/93‑1 (MQ=255)

                                            tCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGTCATGAAAGGTCGCCTg                                             >  2:4319726/1‑93 (MQ=255)

                                            tCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTg                                             <  1:1634166/93‑1 (MQ=255)

                                              cccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGGGCCATGAAAGGTCGCCTGtt                                           >  1:3797870/1‑93 (MQ=255)

                                              cccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTCGCCTGtt                                           >  2:3992433/1‑93 (MQ=255)

                                               ccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTa                                          <  2:1391526/93‑1 (MQ=255)

                                               ccACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTa                                          <  2:3870830/93‑1 (MQ=255)

                                               ccACGGCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTa                                          <  2:2096773/93‑1 (MQ=255)

                                                 aCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGa                                        <  1:3449882/93‑1 (MQ=255)

                                                 aCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGa                                        <  1:869212/93‑1 (MQ=255)

                                                 aCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGa                                        <  2:4674321/93‑1 (MQ=255)

                                                    tCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGc                                     >  2:1411712/1‑93 (MQ=255)

                                                     cAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCt                                    >  2:2136680/1‑93 (MQ=255)

                                                     cAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCt                                    <  1:4194147/93‑1 (MQ=255)

                                                      aaTCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTg                                   >  2:1934618/1‑93 (MQ=255)

                                                      aaTCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTg                                   >  1:2206428/1‑93 (MQ=255)

                                                      aaTCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGGCATGAAAGGTCGCCTGTTAGATGcgg                                   >  1:3722560/1‑91 (MQ=255)

                                                       aTCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGt                                  <  2:2489416/93‑1 (MQ=255)

                                                        tCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTc                                 >  1:4326282/1‑93 (MQ=255)

                                                        tCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTc                                 >  2:1749317/1‑93 (MQ=255)

                                                          aGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTccc                               <  1:3571742/93‑1 (MQ=255)

                                                          aGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGCGCCATGGAAGGTCGCCTGTTAGATGCTGTccc                               >  1:582319/1‑93 (MQ=255)

                                                           gCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCa                              <  2:3777897/93‑1 (MQ=255)

                                                           gCAAGGCGAGATCGAAGCTGAGGCCGACTGGCGGGGTACTAAGCAGGCTGCAGGGTAAGTGCCATGAAAGGGCGCCCGTTTGATGCTGTCCCa                              >  2:4560540/1‑93 (MQ=255)

                                                              aGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGGAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCAccc                           >  1:4216144/1‑91 (MQ=255)

                                                               ggagagaTCGAAGCTGCGGCCTGCTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGCCGCCTGTTAGATGCGGTCCCACTCa                          <  2:1627516/90‑1 (MQ=255)

                                                               ggCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGGAGGGGAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCa                          >  1:1821165/1‑93 (MQ=255)

                                                                   agaTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTc                      >  1:2368437/1‑93 (MQ=255)

                                                                    gaTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTcc                     >  2:2595157/1‑93 (MQ=255)

                                                                     aTCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTccc                    >  1:2461945/1‑93 (MQ=255)

                                                                      tCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCt                   >  2:1533022/1‑93 (MQ=255)

                                                                         aaGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAc                >  1:2745374/1‑93 (MQ=255)

                                                                         aaGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAc                >  1:2137863/1‑93 (MQ=255)

                                                                          aGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGAGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACg               >  2:2029538/1‑93 (MQ=255)

                                                                            cTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACggg             <  2:2827641/93‑1 (MQ=255)

                                                                            cTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACggg             <  1:2644876/93‑1 (MQ=255)

                                                                             tGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGc            >  2:423132/1‑93 (MQ=255)

                                                                              gATGCCGACTGGCGGGCTACTATGCAGGGTGTAGGGTAAGTGCCATGGAATGTCGCCTGTTAGATGCTGTCCCACTCAGCTCCCTCACCGGCg           >  2:253318/1‑93 (MQ=255)

                                                                              gATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCg           >  2:2216338/1‑93 (MQ=255)

                                                                                tGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGtt         <  1:3961199/93‑1 (MQ=255)

                                                                                  ccGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTgg       >  1:2076289/1‑93 (MQ=255)

                                                                                   cGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgc      <  1:4462473/93‑1 (MQ=255)

                                                                                   cGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgc      <  1:1404470/93‑1 (MQ=255)

                                                                                   cGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTACCTAACGGGCGTTggg      >  1:2108508/1‑92 (MQ=255)

                                                                                    gACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGGCGCCTGTTAGATGCTGTCCCCCTCCGTTCCCTAACGGGCGTTGgcg     >  2:856793/1‑93 (MQ=255)

                                                                                     aCTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGgcgc    >  1:2875963/1‑93 (MQ=255)

                                                                                      cTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCgca   <  1:2603974/93‑1 (MQ=255)

                                                                                      cTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTCTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCgca   <  1:3296396/93‑1 (MQ=255)

                                                                                       tggcggGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCgcag  >  2:594253/1‑93 (MQ=255)

TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG  >  NC_000913/3822715‑3822894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  NC_000913/3822712‑3822898

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGC                                                                                               <  SRR4302535.3469088/94‑1 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGC                                                                                               >  SRR4302535.431465/1‑94 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGC                                                                                               <  SRR4302535.4462011/94‑1 (MQ=60)

 GCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCG                                                                                              <  SRR4302535.3456318/94‑1 (MQ=60)

 GCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCG                                                                                              >  SRR4302535.37623/1‑94 (MQ=60)

  CTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGG                                                                                             >  SRR4302535.3642456/1‑94 (MQ=60)

  CTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGG                                                                                             >  SRR4302535.423619/1‑94 (MQ=60)

   TATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGG                                                                                            >  SRR4302535.3592477/1‑94 (MQ=60)

     TCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCT                                                                                          <  SRR4302535.1578201/94‑1 (MQ=60)

     TCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCT                                                                                          <  SRR4302535.2092635/94‑1 (MQ=60)

        GGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACT                                                                                       <  SRR4302535.2224009/94‑1 (MQ=60)

         GTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTA                                                                                      >  SRR4302535.351180/1‑94 (MQ=60)

         GTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCCACTA                                                                                      >  SRR4302535.3928705/1‑94 (MQ=60)

         GTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAACCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTA                                                                                      <  SRR4302535.2532683/94‑1 (MQ=60)

           GCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATG                                                                                    >  SRR4302535.4461923/1‑94 (MQ=60)

            CTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGC                                                                                   >  SRR4302535.2985932/1‑94 (MQ=60)

             TGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCA                                                                                  >  SRR4302535.2616215/1‑94 (MQ=60)

              GGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAG                                                                                 <  SRR4302535.3194084/94‑1 (MQ=60)

              GGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAG                                                                                 >  SRR4302535.2882557/1‑94 (MQ=60)

               GCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGG                                                                                <  SRR4302535.2468407/94‑1 (MQ=60)

                CGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGC                                                                               >  SRR4302535.593253/1‑94 (MQ=60)

                CGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGC                                                                               >  SRR4302535.743287/1‑94 (MQ=60)

                CGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGC                                                                               >  SRR4302535.2850556/1‑94 (MQ=60)

                CGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGC                                                                               >  SRR4302535.627627/1‑94 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              <  SRR4302535.3404740/94‑1 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              <  SRR4302535.2642660/94‑1 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              <  SRR4302535.2384792/94‑1 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCT                                                                              <  SRR4302535.3357701/94‑1 (MQ=60)

                 GAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGAGGGCTACTATGCAGGCG                                                                              >  SRR4302535.859457/1‑94 (MQ=60)

                  AAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTG                                                                             <  SRR4302535.590544/94‑1 (MQ=60)

                  AAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTG                                                                             >  SRR4302535.3431659/1‑94 (MQ=60)

                   AAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGC                                                                            >  SRR4302535.334577/1‑94 (MQ=60)

                   AAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGC                                                                            >  SRR4302535.867951/1‑94 (MQ=60)

                    AGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCA                                                                           >  SRR4302535.1941364/1‑94 (MQ=60)

                    AGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCA                                                                           >  SRR4302535.2394648/1‑94 (MQ=60)

                     ATCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAG                                                                          <  SRR4302535.4478944/94‑1 (MQ=60)

                       CGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGG                                                                        >  SRR4302535.1257815/1‑94 (MQ=60)

                         CAAAACGCAAGGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      >  SRR4302535.3345216/1‑94 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      <  SRR4302535.1461124/94‑1 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      <  SRR4302535.3781355/94‑1 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      <  SRR4302535.1786963/94‑1 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      >  SRR4302535.3941721/1‑94 (MQ=60)

                         CAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTA                                                                      <  SRR4302535.1506754/94‑1 (MQ=60)

                          AAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAA                                                                     >  SRR4302535.3770961/1‑94 (MQ=60)

                          AAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAA                                                                     <  SRR4302535.3371433/94‑1 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    >  SRR4302535.3176712/1‑94 (MQ=60)

                           AAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAG                                                                    <  SRR4302535.2578963/94‑1 (MQ=60)

                            AACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGG                                                                   >  SRR4302535.1076999/1‑94 (MQ=60)

                              CGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGC                                                                 <  SRR4302535.2026360/94‑1 (MQ=60)

                              CGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGC                                                                 <  SRR4302535.1205732/94‑1 (MQ=60)

                              CGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGGGC                                                                 >  SRR4302535.3187166/1‑94 (MQ=60)

                               GCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCC                                                                <  SRR4302535.225794/94‑1 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCA                                                               >  SRR4302535.990466/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGGGCCA                                                               >  SRR4302535.138803/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCA                                                               >  SRR4302535.3432242/1‑94 (MQ=60)

                                CAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGCAAGTGCCC                                                               >  SRR4302535.2079826/1‑94 (MQ=60)

                                 AAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAT                                                              >  SRR4302535.3331793/1‑94 (MQ=60)

                                 AAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAT                                                              <  SRR4302535.3892358/94‑1 (MQ=60)

                                 AAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAT                                                              <  SRR4302535.2147909/94‑1 (MQ=60)

                                 AAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAT                                                              <  SRR4302535.188636/94‑1 (MQ=60)

                                    GGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAA                                                           >  SRR4302535.3352067/1‑94 (MQ=60)

                                    GGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAA                                                           <  SRR4302535.2941492/94‑1 (MQ=60)

                                    GGCCTGCCATATCCCCGCGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAA                                                           <  SRR4302535.1650643/94‑1 (MQ=60)

                                       CTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGGAGGGTAAGTGCCATGAAAGG                                                        >  SRR4302535.1288959/1‑94 (MQ=60)

                                       CTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGG                                                        <  SRR4302535.1424126/94‑1 (MQ=60)

                                        TGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGT                                                       <  SRR4302535.4517888/94‑1 (MQ=60)

                                        TGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGT                                                       <  SRR4302535.3769340/94‑1 (MQ=60)

                                         GCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTC                                                      <  SRR4302535.4648449/94‑1 (MQ=60)

                                         GCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTC                                                      <  SRR4302535.2161175/94‑1 (MQ=60)

                                          CCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCG                                                     <  SRR4302535.3266399/94‑1 (MQ=60)

                                           CTTATCCCCAGGTCAATCAGCAAGGTGAGATTGTATCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTATGTGCCATtaatggtctc                                                    >  SRR4302535.4713643/1‑84 (MQ=58)

                                           CTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGC                                                    >  SRR4302535.328468/1‑94 (MQ=60)

                                           CTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGC                                                    >  SRR4302535.250427/1‑94 (MQ=60)

                                           CTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGGCGC                                                    >  SRR4302535.1280441/1‑94 (MQ=60)

                                           CTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTCGC                                                    >  SRR4302535.3083572/1‑94 (MQ=60)

                                            TTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGTTGCC                                                   >  SRR4302535.2454926/1‑94 (MQ=60)

                                               TCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGT                                                <  SRR4302535.1645974/94‑1 (MQ=60)

                                                CCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTT                                               <  SRR4302535.3452800/94‑1 (MQ=60)

                                                CCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGGGCCATGAAAGGTCGCCTGTT                                               >  SRR4302535.3824051/1‑94 (MQ=60)

                                                 CCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGCCATGAAAGGGCGCCTGTTA                                              >  SRR4302535.4169228/1‑94 (MQ=60)

                                                  CCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAG                                             <  SRR4302535.3496810/94‑1 (MQ=60)

                                                  CCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAG                                             >  SRR4302535.3657203/1‑94 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGGTGGAGGGTAAGTGCCATGAAAGGGTGCCTGTTAGAT                                           >  SRR4302535.2806545/1‑94 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302535.2722291/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302535.3473864/94‑1 (MQ=60)

                                                    ACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGAT                                           <  SRR4302535.875367/94‑1 (MQ=60)

                                                      GTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGC                                         >  SRR4302535.1803504/1‑94 (MQ=60)

                                                       TCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCT                                        <  SRR4302535.3443146/94‑1 (MQ=60)

                                                        CAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTG                                       <  SRR4302535.4222847/94‑1 (MQ=60)

                                                        CAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTG                                       >  SRR4302535.51021/1‑94 (MQ=60)

                                                        CAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTG                                       >  SRR4302535.2222144/1‑94 (MQ=60)

                                                        CAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGGAAGTGGCATGAAAGGTCGCCTGTTAGATGCGG                                       >  SRR4302535.3748347/1‑94 (MQ=60)

                                                          ATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTC                                     >  SRR4302535.2065000/1‑94 (MQ=60)

                                                          ATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTC                                     >  SRR4302535.4229044/1‑94 (MQ=60)

                                                          ATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTC                                     >  SRR4302535.3269378/1‑94 (MQ=60)

                                                          ATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTC                                     >  SRR4302535.4355864/1‑94 (MQ=60)

                                                            CAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCC                                   >  SRR4302535.2761043/1‑94 (MQ=60)

                                                            CAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCC                                   >  SRR4302535.2202856/1‑94 (MQ=60)

                                                            CAGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGCGCCATGGAAGGTCGCCTGTTAGATGCTGTCCC                                   >  SRR4302535.586404/1‑94 (MQ=60)

                                                             AGCAAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCA                                  <  SRR4302535.3596509/94‑1 (MQ=60)

                                                                AAGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGGAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACCC                               >  SRR4302535.4244996/1‑94 (MQ=60)

                                                                 AGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGGAGGGGAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCA                              >  SRR4302535.1834321/1‑94 (MQ=60)

                                                                 AGGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCA                              >  SRR4302535.1834320/1‑94 (MQ=60)

                                                                  GGCGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAG                             <  SRR4302535.4335526/94‑1 (MQ=60)

                                                                    CGAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTT                           <  SRR4302535.234468/94‑1 (MQ=60)

                                                                     GAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATTCTGTCCCACTCAGTTC                          >  SRR4302535.75582/1‑94 (MQ=60)

                                                                     GAGATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTC                          >  SRR4302535.2385442/1‑94 (MQ=60)

                                                                       GATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCC                        >  SRR4302535.2479694/1‑94 (MQ=60)

                                                                       GATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCC                        >  SRR4302535.1871180/1‑94 (MQ=60)

                                                                        ATCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCT                       >  SRR4302535.1433052/1‑94 (MQ=60)

                                                                         TCGAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTA                      <  SRR4302535.1849354/94‑1 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    >  SRR4302535.2764941/1‑94 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    >  SRR4302535.282936/1‑94 (MQ=60)

                                                                           GAAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAAC                    >  SRR4302535.2153259/1‑94 (MQ=60)

                                                                            AAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACG                   <  SRR4302535.3890170/94‑1 (MQ=60)

                                                                            AAGCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACG                   <  SRR4302535.959334/94‑1 (MQ=60)

                                                                              GCTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGG                 <  SRR4302535.2431498/94‑1 (MQ=60)

                                                                               CTGATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGC                <  SRR4302535.2663819/94‑1 (MQ=60)

                                                                                  ATGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGCGCCCGGTTAGATGCTGTCCCACTCAGTTCCTTAACGGGggtg             >  SRR4302535.3797164/1‑90 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            <  SRR4302535.3988250/94‑1 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            <  SRR4302535.2927847/94‑1 (MQ=60)

                                                                                   TGCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCAAGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTG            >  SRR4302535.2531977/1‑94 (MQ=60)

                                                                                    GCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGG           >  SRR4302535.939226/1‑94 (MQ=60)

                                                                                    GCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGG           <  SRR4302535.1078829/94‑1 (MQ=60)

                                                                                    GCCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGG           >  SRR4302535.2091246/1‑94 (MQ=60)

                                                                                    GCCGACTGGCGCGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGG           <  SRR4302535.4125180/94‑1 (MQ=60)

                                                                                     CCGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTACCTAACGGGCGTTGGG          >  SRR4302535.2123714/1‑94 (MQ=60)

                                                                                      CGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCG         <  SRR4302535.4493078/94‑1 (MQ=60)

                                                                                      CGACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCG         <  SRR4302535.1414488/94‑1 (MQ=60)

                                                                                       GACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGC        >  SRR4302535.2896335/1‑94 (MQ=60)

                                                                                        ACTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCA       >  SRR4302535.3494164/1‑94 (MQ=60)

                                                                                         CTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG      >  SRR4302535.210823/1‑94 (MQ=60)

                                                                                         CTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG      <  SRR4302535.2622667/94‑1 (MQ=60)

                                                                                         CTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG      <  SRR4302535.3330339/94‑1 (MQ=60)

                                                                                         CTGGCGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTCTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAG      <  SRR4302535.3319314/94‑1 (MQ=60)

                                                                                             CGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  <  SRR4302535.581096/94‑1 (MQ=60)

GGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCGAGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAAGTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACT  >  NC_000913/3822712‑3822898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`