Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,847,491 T→C L91L (TTA→TTG)  b3668 ← sensor histidine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,847,4910TC100.0% 725.5 / NA 225L91L (TTA→TTG) b3668sensor histidine protein kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (118/107);  total (118/107)

Only 100 of 234 total aligned reads displayed.

GCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAC  >  NC_000913/3847402‑3847580
                                                                                         |                                                                                         
cccgTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCaaa                                                                                        <  1:3578997/92‑1 (MQ=255)
 ccgTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAAc                                                                                       <  2:1655214/93‑1 (MQ=255)
 ccgTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAAc                                                                                       >  2:712145/1‑93 (MQ=255)
   gTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTa                                                                                     <  1:307161/93‑1 (MQ=255)
   gTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTa                                                                                     <  1:4445431/93‑1 (MQ=255)
   cttAACGCCTCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTa                                                                                     <  1:804685/92‑1 (MQ=255)
    ttAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAc                                                                                    >  1:4187935/1‑93 (MQ=255)
      aaCGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCg                                                                                  >  1:3170145/1‑93 (MQ=255)
       aCGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGa                                                                                 >  2:3165089/1‑93 (MQ=255)
       aCGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGa                                                                                 <  2:4674400/93‑1 (MQ=255)
       aCGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGa                                                                                 <  1:392979/93‑1 (MQ=255)
        cgccgcCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGat                                                                                <  2:2589029/93‑1 (MQ=255)
         gccgccCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGatc                                                                               <  1:2376403/93‑1 (MQ=255)
          ccgccCCCTCTAACAGCAAGGTGCGCCAGTCGCTCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGatca                                                                              <  2:4227803/93‑1 (MQ=255)
           cgccCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGatcat                                                                             <  1:3724197/93‑1 (MQ=255)
           cgccCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGatcat                                                                             <  1:3539059/93‑1 (MQ=255)
            ccccccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGatcatc                                                                            <  1:3788269/92‑1 (MQ=255)
             cccccTGTAACAGCAAGGTGCGCCAGTCATGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGAtcatca                                                                           <  2:4602411/93‑1 (MQ=255)
             cccccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGAtcatca                                                                           >  1:2711310/1‑93 (MQ=255)
             cccccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGAtcatca                                                                           >  1:1421255/1‑93 (MQ=255)
               cccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAAt                                                                         >  2:1386824/1‑93 (MQ=255)
                ccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATa                                                                        <  1:2869624/93‑1 (MQ=255)
                ccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATa                                                                        >  2:1631347/1‑93 (MQ=255)
                 cTGTGACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATaa                                                                       <  1:91543/93‑1 (MQ=255)
                 cTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATaa                                                                       >  2:3488520/1‑93 (MQ=255)
                  tGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAAc                                                                      >  1:2149165/1‑93 (MQ=255)
                    tAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACgg                                                                    >  1:3468211/1‑93 (MQ=255)
                        aGCAAGGTGCGCCAGTCACGCTGATGGCGATATCGCGAGATCAGCGCTACGGACAGTAACGTCAGCAAACTACCGATCATCAATAACGGaaaa                                                                <  1:4667912/93‑1 (MQ=255)
                        aGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGaaaa                                                                >  2:278957/1‑93 (MQ=255)
                        aGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGaaaa                                                                <  1:4065637/93‑1 (MQ=255)
                          cAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATg                                                              <  2:3524844/93‑1 (MQ=255)
                          cAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATg                                                              <  2:2324363/93‑1 (MQ=255)
                           aaGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATgg                                                             <  2:3764/93‑1 (MQ=255)
                           aaGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATgg                                                             >  2:1542890/1‑93 (MQ=255)
                            aGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATggg                                                            >  2:431021/1‑93 (MQ=255)
                            aGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATggg                                                            <  2:4201285/93‑1 (MQ=255)
                            aGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATggg                                                            >  1:4029188/1‑93 (MQ=255)
                            aGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATggg                                                            >  2:4655941/1‑93 (MQ=255)
                              gTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGtt                                                          <  2:2711574/93‑1 (MQ=255)
                                gcgcCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTaa                                                        <  2:2538677/93‑1 (MQ=255)
                                gcgcCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTaa                                                        >  2:3870977/1‑93 (MQ=255)
                                  gcCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAac                                                      <  2:2149994/93‑1 (MQ=255)
                                   ccAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAacc                                                     >  1:4023850/1‑93 (MQ=255)
                                   ccAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAacc                                                     >  1:2206103/1‑93 (MQ=255)
                                    cAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccg                                                    <  1:210248/93‑1 (MQ=255)
                                     aGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgg                                                   <  2:4321158/93‑2 (MQ=255)
                                      gTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgac                                                  >  1:2614492/1‑93 (MQ=255)
                                      gTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgac                                                  <  2:849060/93‑1 (MQ=255)
                                      gTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgac                                                  >  1:2014943/1‑93 (MQ=255)
                                      gTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgac                                                  >  2:1038575/1‑93 (MQ=255)
                                      gTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgac                                                  >  2:118173/1‑93 (MQ=255)
                                       tCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgacc                                                 >  2:4477060/1‑93 (MQ=255)
                                        cACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgaccg                                                <  2:3767951/93‑1 (MQ=255)
                                        cACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgaccg                                                >  1:1920084/1‑93 (MQ=255)
                                          cGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGcc                                              <  2:1103016/93‑1 (MQ=255)
                                          cGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGcc                                              >  1:4239465/1‑93 (MQ=255)
                                           gCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCt                                             <  1:3770131/93‑1 (MQ=255)
                                           gCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAAACGACCGCCt                                             >  2:24761/1‑93 (MQ=255)
                                            cTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTg                                            <  2:3253756/93‑1 (MQ=255)
                                             tGATGGCGCTAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGc                                           >  2:131709/1‑93 (MQ=255)
                                             tGATGGCGATAGCGCGAGATCAGCTCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGc                                           <  1:3456403/93‑1 (MQ=255)
                                               aTGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGt                                         <  1:56349/93‑1 (MQ=255)
                                               aTGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGt                                         <  2:4085015/93‑1 (MQ=255)
                                                 ggCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTa                                       >  1:3870286/1‑93 (MQ=255)
                                                 ggCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTa                                       <  1:449388/93‑1 (MQ=255)
                                                   cGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGc                                     <  2:136194/93‑1 (MQ=255)
                                                    gATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGcc                                    >  1:670986/1‑93 (MQ=255)
                                                    gATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGcc                                    >  1:1213327/1‑93 (MQ=255)
                                                     aTAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCa                                   >  2:586318/1‑93 (MQ=255)
                                                        gcgcGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTa                                >  1:1879452/1‑93 (MQ=255)
                                                          gcgAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTaaa                              <  1:4204265/93‑1 (MQ=255)
                                                          gcgAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTCAAACCGACCGCCTGCGTTAGCCAGTaaa                              <  1:4362070/93‑1 (MQ=255)
                                                            gagaTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATc                            <  1:4523818/93‑1 (MQ=255)
                                                            gagaTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATc                            >  1:4402831/1‑93 (MQ=255)
                                                            gagaTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATc                            >  1:2551928/1‑93 (MQ=255)
                                                             agaTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATca                           >  2:4041994/1‑93 (MQ=255)
                                                             agaTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATca                           >  1:1002801/1‑93 (MQ=255)
                                                              gaTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcag                          >  2:323462/1‑93 (MQ=255)
                                                               aTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagc                         <  2:4397844/93‑1 (MQ=255)
                                                               aTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagc                         <  2:4005461/93‑1 (MQ=255)
                                                                tCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagca                        >  2:929958/1‑93 (MQ=255)
                                                                tCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagca                        <  2:2883249/93‑1 (MQ=255)
                                                                 cAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagcag                       <  2:666714/93‑1 (MQ=255)
                                                                 cAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagcag                       <  1:1781085/93‑1 (MQ=255)
                                                                     gcTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCAc                   <  2:2684431/93‑1 (MQ=255)
                                                                     gcTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCAc                   >  1:4282089/1‑93 (MQ=255)
                                                                      cTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACt                  >  2:209952/1‑93 (MQ=255)
                                                                       tACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTc                 <  1:279324/93‑1 (MQ=255)
                                                                        aCGGGCAGTACCGTCAGCAAACTACCGATCATCAATAACGGAAAAGGGGTTAAACCGCCCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTcc                <  1:20648/93‑1 (MQ=255)
                                                                        aCGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTcc                >  1:1565716/1‑93 (MQ=255)
                                                                             cAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGcc           >  1:251269/1‑93 (MQ=255)
                                                                              aGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGccc          <  2:4436686/93‑1 (MQ=255)
                                                                              aGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGccc          >  1:1636998/1‑93 (MQ=255)
                                                                                 aaCGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGc       <  2:3045384/93‑1 (MQ=255)
                                                                                  aCGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCa      <  2:3560782/93‑1 (MQ=255)
                                                                                   cGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCaa     <  1:3129419/93‑1 (MQ=255)
                                                                                    gTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAAt    >  2:3351889/1‑93 (MQ=255)
                                                                                     tCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATa   <  1:1017855/93‑1 (MQ=255)
                                                                                      cAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAc  <  2:3777153/93‑1 (MQ=255)
                                                                                      cAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAc  >  2:3937626/1‑93 (MQ=255)
                                                                                         |                                                                                         
GCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAC  >  NC_000913/3847402‑3847580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGG  >  NC_000913/3847399‑3847582
                                                                                            |                                                                                           
GCCGCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCA                                                                                            >  SRR4302535.4401287/1‑94 (MQ=60)
 CCGCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAA                                                                                           <  SRR4302535.2767215/94‑1 (MQ=60)
  CGCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAA                                                                                          >  SRR4302535.4243723/1‑94 (MQ=60)
   GCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAAC                                                                                         >  SRR4302535.4457681/1‑94 (MQ=60)
   GCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAAC                                                                                         <  SRR4302535.3906589/94‑1 (MQ=60)
   CCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAAC                                                                                         <  SRR4302535.3603805/94‑1 (MQ=60)
    CCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACT                                                                                        <  SRR4302535.3449338/94‑1 (MQ=60)
     CGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTA                                                                                       >  SRR4302535.1877207/1‑94 (MQ=60)
      GTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAC                                                                                      <  SRR4302535.4475917/94‑1 (MQ=60)
      GTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAC                                                                                      >  SRR4302535.4216598/1‑94 (MQ=60)
      GTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAC                                                                                      <  SRR4302535.309288/94‑1 (MQ=60)
      CTTAACGCCTCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAC                                                                                      <  SRR4302535.810335/94‑1 (MQ=60)
       TTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACC                                                                                     <  SRR4302535.4468225/94‑1 (MQ=60)
       TTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACC                                                                                     <  SRR4302535.1621094/94‑1 (MQ=60)
        TAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCG                                                                                    >  SRR4302535.3192099/1‑94 (MQ=60)
         AACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGA                                                                                   <  SRR4302535.4309830/94‑1 (MQ=60)
          ACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGAT                                                                                  <  SRR4302535.1756177/94‑1 (MQ=60)
          ACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGAT                                                                                  <  SRR4302535.395717/94‑1 (MQ=60)
            GCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCA                                                                                <  SRR4302535.3595752/94‑1 (MQ=60)
            GCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCA                                                                                >  SRR4302535.4586086/1‑94 (MQ=60)
            GCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCA                                                                                <  SRR4302535.2393474/94‑1 (MQ=60)
              CGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATC                                                                              >  SRR4302535.2679434/1‑94 (MQ=60)
              CGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATC                                                                              <  SRR4302535.3563628/94‑1 (MQ=60)
              CGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATC                                                                              <  SRR4302535.3749996/94‑1 (MQ=60)
              CGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATC                                                                              <  SRR4302535.4700608/94‑1 (MQ=60)
               GCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCA                                                                             >  SRR4302535.1431402/1‑94 (MQ=60)
               GCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCA                                                                             >  SRR4302535.2730687/1‑94 (MQ=60)
               CCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCA                                                                             <  SRR4302535.3814397/94‑1 (MQ=60)
                CCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAA                                                                            >  SRR4302535.2031264/1‑94 (MQ=60)
                CCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAA                                                                            >  SRR4302535.3156465/1‑94 (MQ=60)
                CCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAA                                                                            <  SRR4302535.948320/94‑1 (MQ=60)
                CCCCCTGTAACAGCAAGGTGAGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAA                                                                            >  SRR4302535.2436871/1‑94 (MQ=60)
                 CCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAAT                                                                           >  SRR4302535.3793996/1‑94 (MQ=60)
                 CCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAAT                                                                           <  SRR4302535.4600731/94‑1 (MQ=60)
                 CCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAAT                                                                           >  SRR4302535.1016747/1‑94 (MQ=60)
                   CCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAA                                                                         <  SRR4302535.2889946/94‑1 (MQ=60)
                    CTGTGACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAAC                                                                        <  SRR4302535.92190/94‑1 (MQ=60)
                    CTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAAC                                                                        >  SRR4302535.383852/1‑94 (MQ=60)
                    CTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAAC                                                                        >  SRR4302535.4018743/1‑94 (MQ=60)
                    CTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAAC                                                                        <  SRR4302535.959129/94‑1 (MQ=60)
                    CTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAAC                                                                        <  SRR4302535.2162571/94‑1 (MQ=60)
                    CTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAAC                                                                        >  SRR4302535.2164620/1‑94 (MQ=60)
                     TGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACG                                                                       >  SRR4302535.2488943/1‑94 (MQ=60)
                     TGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACG                                                                       >  SRR4302535.1683456/1‑94 (MQ=60)
                     TGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACG                                                                       >  SRR4302535.1422086/1‑94 (MQ=60)
                      GTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGG                                                                      >  SRR4302535.3492312/1‑94 (MQ=60)
                        AACAGCAAGGTGGACCGGTCACACTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTATCGTCAGCAAACTACCGATCATCAATAACGGAA                                                                    <  SRR4302535.1614289/94‑1 (MQ=60)
                          CAGCAAGGTGCGCCAGTCACTCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAA                                                                  >  SRR4302535.70419/1‑94 (MQ=60)
                           AGCAGGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAAT                                                                 <  SRR4302535.4274029/94‑1 (MQ=60)
                           AGCAAGGTGCGCCAGTCACGCTGATGGCGATATCGCGAGATCAGCGCTACGGACAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAAT                                                                 <  SRR4302535.4699455/94‑1 (MQ=60)
                           AGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAAT                                                                 >  SRR4302535.2108853/1‑94 (MQ=60)
                           AGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAAT                                                                 <  SRR4302535.4093456/94‑1 (MQ=60)
                             CAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGG                                                               >  SRR4302535.2704247/1‑94 (MQ=60)
                              AAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGG                                                              >  SRR4302535.4056752/1‑94 (MQ=60)
                              AAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGG                                                              >  SRR4302535.4552187/1‑94 (MQ=60)
                                GGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTT                                                            >  SRR4302535.4398177/1‑94 (MQ=60)
                                  TGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAA                                                          >  SRR4302535.4502714/1‑94 (MQ=60)
                                    CGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAAC                                                        <  SRR4302535.773045/94‑1 (MQ=60)
                                     GCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACC                                                       >  SRR4302535.2221816/1‑94 (MQ=60)
                                     GCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACC                                                       >  SRR4302535.1969042/1‑94 (MQ=60)
                                     GCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACC                                                       >  SRR4302535.4051381/1‑94 (MQ=60)
                                      CCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCG                                                      >  SRR4302535.4231803/1‑94 (MQ=60)
                                       CAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGA                                                     <  SRR4302535.211692/94‑1 (MQ=60)
                                        AGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGAC                                                    >  SRR4302535.2029457/1‑94 (MQ=60)
                                        AGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGAC                                                    >  SRR4302535.4328277/1‑94 (MQ=60)
                                        AGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGAC                                                    >  SRR4302535.2633241/1‑94 (MQ=60)
                                          TCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCG                                                  >  SRR4302535.1933923/1‑94 (MQ=60)
                                          GCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCG                                                  <  SRR4302535.1985764/94‑1 (MQ=60)
                                            ACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCC                                                >  SRR4302535.4268485/1‑94 (MQ=60)
                                             CGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCT                                               <  SRR4302535.2794874/94‑1 (MQ=60)
                                              GCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTG                                              <  SRR4302535.3796149/94‑1 (MQ=60)
                                                TGATGGCGATAGCGCGAGATCAGCTCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCG                                            <  SRR4302535.3480428/94‑1 (MQ=60)
                                                 GATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGCTCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGT                                           >  SRR4302535.3589767/1‑94 (MQ=60)
                                                  ATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTT                                          <  SRR4302535.56757/94‑1 (MQ=60)
                                                   TGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTA                                         >  SRR4302535.3896885/1‑94 (MQ=60)
                                                    GGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAG                                        <  SRR4302535.452535/94‑1 (MQ=60)
                                                    GGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAG                                        <  SRR4302535.3525219/94‑1 (MQ=60)
                                                      CGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCC                                      <  SRR4302535.2250817/94‑1 (MQ=60)
                                                      CGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCC                                      >  SRR4302535.292409/1‑94 (MQ=60)
                                                      CGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCC                                      >  SRR4302535.1222031/1‑94 (MQ=60)
                                                      CGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCC                                      >  SRR4302535.675672/1‑94 (MQ=60)
                                                       GATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCA                                     >  SRR4302535.1929397/1‑94 (MQ=60)
                                                          AGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTA                                  >  SRR4302535.1892995/1‑94 (MQ=60)
                                                           GCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAA                                 >  SRR4302535.3697848/1‑94 (MQ=60)
                                                            CGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAA                                >  SRR4302535.4062101/1‑94 (MQ=60)
                                                            CGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAA                                >  SRR4302535.2135835/1‑94 (MQ=60)
                                                             GCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAAT                               <  SRR4302535.4233039/94‑1 (MQ=60)
                                                             GCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTCAAACCGACCGCCTGCGTTAGCCAGTAAAT                               <  SRR4302535.4391921/94‑1 (MQ=60)
                                                              CGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATC                              >  SRR4302535.4432962/1‑94 (MQ=60)
                                                              CGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATC                              >  SRR4302535.2570302/1‑94 (MQ=60)
                                                               GAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCA                             <  SRR4302535.613993/94‑1 (MQ=60)
                                                               GAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCA                             >  SRR4302535.1009921/1‑94 (MQ=60)
                                                               GAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCA                             <  SRR4302535.4554812/94‑1 (MQ=60)
                                                               GAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCA                             <  SRR4302535.3801964/94‑1 (MQ=60)
                                                                AGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAG                            >  SRR4302535.1487857/1‑94 (MQ=60)
                                                                  ATCAGCGCTACGGGCAGTAACGTCAGGAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCA                          <  SRR4302535.1356093/94‑1 (MQ=60)
                                                                  ATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCA                          <  SRR4302535.4132738/94‑1 (MQ=60)
                                                                  ATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCA                          <  SRR4302535.3801614/94‑1 (MQ=60)
                                                                    CAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGC                        <  SRR4302535.1793974/94‑1 (MQ=60)
                                                                     AGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCC                       >  SRR4302535.1155672/1‑94 (MQ=60)
                                                                     AGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCC                       >  SRR4302535.2867839/1‑94 (MQ=60)
                                                                       CGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCAC                     >  SRR4302535.4311386/1‑94 (MQ=60)
                                                                       CGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCAC                     >  SRR4302535.834865/1‑94 (MQ=60)
                                                                       CGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCAC                     >  SRR4302535.3484321/1‑94 (MQ=60)
                                                                          TACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCC                  <  SRR4302535.4054161/94‑1 (MQ=60)
                                                                          TACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCC                  <  SRR4302535.281272/94‑1 (MQ=60)
                                                                          TACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCC                  >  SRR4302535.1577019/1‑94 (MQ=60)
                                                                          TACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCC                  >  SRR4302535.3134529/1‑94 (MQ=60)
                                                                           ACGGGCAGTACCGTCAGCAAACTACCGATCATCAATAACGGAAAAGGGGTTAAACCGCCCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCG                 <  SRR4302535.20802/94‑1 (MQ=60)
                                                                            CGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGC                <  SRR4302535.2598111/94‑1 (MQ=60)
                                                                               GCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCC             >  SRR4302535.252999/1‑94 (MQ=60)
                                                                                CAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCC            >  SRR4302535.1648823/1‑94 (MQ=60)
                                                                                 AGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTCGCCAGTAAATCAGCAGCCACTCCGCGCCCA           >  SRR4302535.2549643/1‑94 (MQ=60)
                                                                                   TAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGC         >  SRR4302535.3455411/1‑94 (MQ=60)
                                                                                      CGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAAT      <  SRR4302535.3151215/94‑1 (MQ=60)
                                                                                      CGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAAT      <  SRR4302535.4035605/94‑1 (MQ=60)
                                                                                      CGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAAT      <  SRR4302535.4198959/94‑1 (MQ=60)
                                                                                        TCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAC    >  SRR4302535.2208633/1‑94 (MQ=60)
                                                                                        TCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAC    <  SRR4302535.1025090/94‑1 (MQ=60)
                                                                                         CAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCGAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACG   <  SRR4302535.2908762/94‑1 (MQ=60)
                                                                                         CAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACG   >  SRR4302535.2216517/1‑94 (MQ=60)
                                                                                         CAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACG   >  SRR4302535.3590589/1‑94 (MQ=60)
                                                                                         CAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACG   >  SRR4302535.4404351/1‑94 (MQ=60)
                                                                                          AGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGG  <  SRR4302535.666106/94‑1 (MQ=60)
                                                                                            |                                                                                           
GCCGCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGG  >  NC_000913/3847399‑3847582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑