Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,851,510	T→C	intergenic (‑895/‑33)	ivbL ← / → emrD	ilvB operon leader peptide/2‑module integral membrane pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,851,510	0	T	C	99.6%	737.7 / ‑4.5	231	intergenic (‑895/‑33)	ivbL/emrD	ilvB operon leader peptide/2‑module integral membrane pump
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (91/139); minor base A (0/1); total (91/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.32e-01

Only 100 of 234 total aligned reads displayed.

GCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTG  >  NC_000913/3851420‑3851599

 ccGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGAc                                                                                        <  2:108919/93‑1 (MQ=255)

 ccGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGAc                                                                                        >  1:1832716/1‑93 (MQ=255)

  cGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACt                                                                                       <  2:3876448/93‑1 (MQ=255)

  cGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACt                                                                                       >  2:1045498/1‑93 (MQ=255)

   gCTAAGCAATTCCCCATTCCTGGCGTATATCTGGCTAGCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACtt                                                                                      <  1:2016986/93‑1 (MQ=255)

   gCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACtt                                                                                      >  2:2520468/1‑93 (MQ=255)

   gCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACtt                                                                                      <  1:336695/93‑1 (MQ=255)

    cTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTa                                                                                     <  1:905153/93‑1 (MQ=255)

    cTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTa                                                                                     <  1:338313/93‑1 (MQ=255)

    cTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTa                                                                                     <  1:1004613/93‑1 (MQ=255)

    cTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTa                                                                                     <  2:132124/93‑1 (MQ=255)

      aaaCAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTAtt                                                                                   <  1:3950414/93‑1 (MQ=255)

       aaCAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTc                                                                                  <  1:1473784/93‑1 (MQ=255)

       aaCAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTc                                                                                  >  1:732374/1‑93 (MQ=255)

       aaCAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTc                                                                                  >  1:525835/1‑93 (MQ=255)

       aaCAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTc                                                                                  <  1:308244/93‑1 (MQ=255)

       aaCAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTc                                                                                  <  2:1870072/93‑1 (MQ=255)

         cAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTa                                                                                <  2:2094850/93‑1 (MQ=255)

          aaTTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTaa                                                                               <  2:2952195/93‑1 (MQ=255)

          aaTTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTaa                                                                               <  2:812834/93‑1 (MQ=255)

          aaTTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTaa                                                                               <  2:4003535/93‑1 (MQ=255)

           aTTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAat                                                                              <  2:2861541/93‑1 (MQ=255)

           aTTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAat                                                                              <  1:4156057/93‑1 (MQ=255)

            ttCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAatt                                                                             <  1:2419073/93‑1 (MQ=255)

             tCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAatta                                                                            <  1:1190668/93‑1 (MQ=255)

             tCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAatta                                                                            <  1:406047/93‑1 (MQ=255)

             tCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAatta                                                                            <  2:3667947/93‑1 (MQ=255)

             tCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAatta                                                                            <  1:3483935/93‑1 (MQ=255)

              ccccATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAattat                                                                           >  2:3072765/1‑93 (MQ=255)

              ccccATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAattat                                                                           <  1:2138464/93‑1 (MQ=255)

               cccATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAattatt                                                                          <  2:864874/93‑1 (MQ=255)

               cccATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAattatt                                                                          <  2:67084/93‑1 (MQ=255)

               cccATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAattatt                                                                          >  2:2619304/1‑93 (MQ=255)

                ccATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTAttt                                                                         <  2:1232165/93‑1 (MQ=255)

                 cATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTAtttt                                                                        >  2:530950/1‑93 (MQ=255)

                 cATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTAtttt                                                                        <  1:700178/93‑1 (MQ=255)

                 cATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTAtttt                                                                        >  2:276617/1‑93 (MQ=255)

                  aTTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTAttttt                                                                       <  1:401954/93‑1 (MQ=255)

                   ttCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTta                                                                      <  2:4143930/93‑1 (MQ=255)

                   ttCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTta                                                                      >  2:1713829/1‑93 (MQ=255)

                    tCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTtat                                                                     >  1:1363464/1‑93 (MQ=255)

                      cTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATaa                                                                   >  1:378495/1‑93 (MQ=255)

                      cTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATaa                                                                   >  1:3162393/1‑93 (MQ=255)

                       tGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATaaa                                                                  >  1:713074/1‑93 (MQ=255)

                        ggCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATaaaa                                                                 >  2:2000806/1‑93 (MQ=255)

                          cGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGc                                                               <  1:427739/93‑1 (MQ=255)

                            tatcTCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCAt                                                             <  2:1218521/93‑1 (MQ=255)

                            tataTCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCAt                                                             <  2:2279065/93‑1 (MQ=255)

                            tataTCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCAt                                                             <  2:2423042/93‑1 (MQ=255)

                            tataTCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCAt                                                             >  1:2294516/1‑93 (MQ=255)

                                  tGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGa                                                       <  2:2704569/93‑1 (MQ=255)

                                   ggCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGAt                                                      <  1:340044/93‑1 (MQ=255)

                                   ggCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGAt                                                      >  1:4191079/1‑93 (MQ=255)

                                     cTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATaa                                                    >  2:516227/1‑93 (MQ=255)

                                      tAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAAt                                                   >  1:449989/1‑93 (MQ=255)

                                      tAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAAt                                                   >  1:2682817/1‑93 (MQ=255)

                                      tAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAAt                                                   >  1:3547491/1‑93 (MQ=255)

                                       aaCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGGATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATg                                                  >  1:2510780/1‑93 (MQ=255)

                                       aaCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATg                                                  <  2:3956172/93‑1 (MQ=255)

                                       aaCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATg                                                  <  1:1888663/93‑1 (MQ=255)

                                       aaCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATg                                                  <  1:1630902/93‑1 (MQ=255)

                                       aaCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATg                                                  <  1:1134941/93‑1 (MQ=255)

                                        aCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGa                                                 <  2:3447764/93‑1 (MQ=255)

                                        aCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGa                                                 <  2:1479573/93‑1 (MQ=255)

                                        aCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGa                                                 <  2:4117101/93‑1 (MQ=255)

                                         cATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGaa                                                >  1:2595360/1‑93 (MQ=255)

                                          aTTCATCATTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGaaa                                               >  2:4557069/1‑93 (MQ=255)

                                           ttCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGaaaa                                              >  1:2097118/1‑93 (MQ=255)

                                             catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAgg                                            >  1:4576404/1‑93 (MQ=255)

                                              atcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGc                                           <  2:1613297/93‑1 (MQ=255)

                                                 aaTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCaaa                                        <  1:4375933/93‑1 (MQ=255)

                                                 aaTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCaaa                                        >  1:2933289/1‑93 (MQ=255)

                                                  aTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAg                                       <  1:3683763/93‑1 (MQ=255)

                                                   tgtgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAATCATCCGTGATAATGAAAAGGCAAAGa                                      <  1:333090/93‑1 (MQ=255)

                                                     tgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGaaa                                    <  2:4037134/93‑1 (MQ=255)

                                                     tgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGaaa                                    <  2:133545/93‑1 (MQ=255)

                                                     tgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGaaa                                    >  1:817041/1‑93 (MQ=255)

                                                     tgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGaaa                                    <  2:2149036/93‑1 (MQ=255)

                                                     tgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGaaa                                    <  1:4415309/93‑1 (MQ=255)

                                                      gATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAAc                                   <  1:2321705/93‑1 (MQ=255)

                                                       aTAGATTCCTCTCCCGCATTTATAGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACg                                  >  2:3781850/1‑93 (MQ=255)

                                                           aTTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCaa                              <  1:4305027/93‑1 (MQ=255)

                                                               ctctCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTg                          >  2:1124761/1‑93 (MQ=255)

                                                                tctcCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGt                         >  1:4094983/1‑93 (MQ=255)

                                                                tctcCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGt                         >  1:2576165/1‑93 (MQ=255)

                                                                   cccGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAt                      >  2:1377345/1‑93 (MQ=255)

                                                                     cGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTg                    >  1:1556926/1‑93 (MQ=255)

                                                                       cATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGAt                  <  2:2779461/93‑1 (MQ=255)

                                                                       cATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGAt                  >  1:2251729/1‑93 (MQ=255)

                                                                        aTTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATg                 <  1:471848/93‑1 (MQ=255)

                                                                         tttATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGt                >  2:2382890/1‑93 (MQ=255)

                                                                         tttATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGt                <  1:3053304/93‑1 (MQ=255)

                                                                         tttATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGt                <  2:1529843/93‑1 (MQ=255)

                                                                           tATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTg              <  1:2791288/93‑1 (MQ=255)

                                                                              gggAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTa           <  1:2453108/93‑1 (MQ=255)

                                                                                 aaTGCGTAGCGACTTATGCTAATTATTTTTATAAAAGCATCGGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTa        <  2:4642798/93‑1 (MQ=255)

                                                                                  aTGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTAc       >  2:4368668/1‑93 (MQ=255)

                                                                                  aTGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTAc       >  2:1752805/1‑93 (MQ=255)

                                                                                   tGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACt      <  1:291902/93‑1 (MQ=255)

                                                                                      gTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGt   <  2:2915612/93‑1 (MQ=255)

GCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTG  >  NC_000913/3851420‑3851599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCG  >  NC_000913/3851418‑3851603

CTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCG                                                                                              <  SRR4302535.3024345/94‑1 (MQ=60)

 TGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGA                                                                                             <  SRR4302535.3724435/94‑1 (MQ=60)

  GCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGAC                                                                                            >  SRR4302535.1845942/1‑94 (MQ=60)

   CCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACT                                                                                           <  SRR4302535.1087810/94‑1 (MQ=60)

   CCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACT                                                                                           >  SRR4302535.4059720/1‑94 (MQ=60)

     GCTAAGCAATTCCCCATTCCTGGCGTATATCTGGCTAGCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTA                                                                                         <  SRR4302535.2031514/94‑1 (MQ=60)

     GCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTA                                                                                         <  SRR4302535.339022/94‑1 (MQ=60)

     GCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTA                                                                                         >  SRR4302535.1913731/1‑94 (MQ=60)

     GCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTA                                                                                         <  SRR4302535.1856301/94‑1 (MQ=60)

      CTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTAT                                                                                        <  SRR4302535.911577/94‑1 (MQ=60)

      CTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTAT                                                                                        <  SRR4302535.1011747/94‑1 (MQ=60)

      CTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTAT                                                                                        <  SRR4302535.4653382/94‑1 (MQ=60)

      CTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTAT                                                                                        <  SRR4302535.340653/94‑1 (MQ=60)

      CTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTAT                                                                                        <  SRR4302535.2258358/94‑1 (MQ=60)

        AAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTC                                                                                      <  SRR4302535.3977385/94‑1 (MQ=60)

        AAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTC                                                                                      >  SRR4302535.737516/1‑94 (MQ=60)

        AAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTC                                                                                      >  SRR4302535.529506/1‑94 (MQ=60)

         AACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCT                                                                                     <  SRR4302535.310380/94‑1 (MQ=60)

         AACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCT                                                                                     <  SRR4302535.1484336/94‑1 (MQ=60)

          ACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTA                                                                                    >  SRR4302535.2433978/1‑94 (MQ=60)

           CAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAA                                                                                   <  SRR4302535.1632619/94‑1 (MQ=60)

            AATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAAT                                                                                  >  SRR4302535.260179/1‑94 (MQ=60)

             ATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATT                                                                                 <  SRR4302535.4026107/94‑1 (MQ=60)

             ATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATT                                                                                 <  SRR4302535.3422640/94‑1 (MQ=60)

             ATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATT                                                                                 <  SRR4302535.4184508/94‑1 (MQ=60)

              TTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTA                                                                                <  SRR4302535.2436490/94‑1 (MQ=60)

               TCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTAT                                                                               <  SRR4302535.3508125/94‑1 (MQ=60)

               TCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTAT                                                                               <  SRR4302535.408889/94‑1 (MQ=60)

               TCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTAT                                                                               <  SRR4302535.1199193/94‑1 (MQ=60)

                CCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATT                                                                              <  SRR4302535.2153864/94‑1 (MQ=60)

                  CCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTT                                                                            <  SRR4302535.2037869/94‑1 (MQ=60)

                   CATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTT                                                                           <  SRR4302535.705089/94‑1 (MQ=60)

                   CATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTT                                                                           <  SRR4302535.1580745/94‑1 (MQ=60)

                    ATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTA                                                                          <  SRR4302535.404772/94‑1 (MQ=60)

                     TTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTAT                                                                         <  SRR4302535.3265968/94‑1 (MQ=60)

                     TTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTAT                                                                         >  SRR4302535.1373215/1‑94 (MQ=60)

                     TTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTAT                                                                         >  SRR4302535.3541571/1‑94 (MQ=60)

                       CCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAA                                                                       <  SRR4302535.416980/94‑1 (MQ=60)

                       CCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAA                                                                       >  SRR4302535.3184327/1‑94 (MQ=60)

                       CCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAA                                                                       >  SRR4302535.1914767/1‑94 (MQ=60)

                       CCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAA                                                                       >  SRR4302535.381116/1‑94 (MQ=60)

                       CCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAA                                                                       >  SRR4302535.909153/1‑94 (MQ=60)

                        CTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAA                                                                      <  SRR4302535.3418836/94‑1 (MQ=60)

                        CTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAA                                                                      >  SRR4302535.718082/1‑94 (MQ=60)

                        CTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAA                                                                      <  SRR4302535.3789107/94‑1 (MQ=60)

                          GGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAG                                                                    <  SRR4302535.522858/94‑1 (MQ=60)

                            CGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCA                                                                  <  SRR4302535.430713/94‑1 (MQ=60)

                             GTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCAT                                                                 >  SRR4302535.2318975/1‑94 (MQ=60)

                             GTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCAT                                                                 <  SRR4302535.2550917/94‑1 (MQ=60)

                             GTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCAT                                                                 >  SRR4302535.2310925/1‑94 (MQ=60)

                              TATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATC                                                                <  SRR4302535.2114066/94‑1 (MQ=60)

                              TATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATC                                                                >  SRR4302535.794957/1‑94 (MQ=60)

                               ATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCC                                                               >  SRR4302535.2509209/1‑94 (MQ=60)

                               ATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCC                                                               >  SRR4302535.1213968/1‑94 (MQ=60)

                                 ATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGT                                                             >  SRR4302535.1171382/1‑94 (MQ=60)

                                    TGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGAT                                                          >  SRR4302535.4219762/1‑94 (MQ=60)

                                     GGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATA                                                         >  SRR4302535.2951872/1‑94 (MQ=60)

                                     GGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATA                                                         <  SRR4302535.342402/94‑1 (MQ=60)

                                      GCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAA                                                        <  SRR4302535.2835818/94‑1 (MQ=60)

                                       CTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAAT                                                       >  SRR4302535.3572118/1‑94 (MQ=60)

                                       CTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAAT                                                       >  SRR4302535.2702006/1‑94 (MQ=60)

                                       CTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAAT                                                       >  SRR4302535.453140/1‑94 (MQ=60)

                                       CTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAAT                                                       <  SRR4302535.3383739/94‑1 (MQ=60)

                                        TAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGGATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATG                                                      >  SRR4302535.2528879/1‑94 (MQ=60)

                                         AACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGA                                                     <  SRR4302535.1143038/94‑1 (MQ=60)

                                         AACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGA                                                     <  SRR4302535.1642680/94‑1 (MQ=60)

                                         AACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGA                                                     <  SRR4302535.1902267/94‑1 (MQ=60)

                                          ACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAA                                                    <  SRR4302535.4220239/94‑1 (MQ=60)

                                          ACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAA                                                    >  SRR4302535.2614004/1‑94 (MQ=60)

                                            ATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAA                                                  >  SRR4302535.2112242/1‑94 (MQ=60)

                                              TCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGG                                                >  SRR4302535.4607587/1‑94 (MQ=60)

                                              TCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGG                                                >  SRR4302535.2451902/1‑94 (MQ=60)

                                                ATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCA                                              <  SRR4302535.446308/94‑1 (MQ=60)

                                                  CAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAA                                            >  SRR4302535.2953935/1‑94 (MQ=60)

                                                  CAATGTGATAGATTCCTCTACCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAA                                            >  SRR4302535.409838/1‑94 (MQ=60)

                                                   AATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAG                                           <  SRR4302535.4405874/94‑1 (MQ=60)

                                                    ATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGA                                          >  SRR4302535.895944/1‑94 (MQ=60)

                                                    ATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGA                                          <  SRR4302535.3709251/94‑1 (MQ=60)

                                                     TGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAATCATCCGTGATAATGAAAAGGCAAAGAA                                         <  SRR4302535.335396/94‑1 (MQ=60)

                                                     TGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAA                                         <  SRR4302535.4458840/94‑1 (MQ=60)

                                                      GTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAA                                        >  SRR4302535.822771/1‑94 (MQ=60)

                                                       TGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAAC                                       <  SRR4302535.4445526/94‑1 (MQ=60)

                                                        GATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACG                                      <  SRR4302535.2338320/94‑1 (MQ=60)

                                                        GATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACG                                      <  SRR4302535.1202871/94‑1 (MQ=60)

                                                        GATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACG                                      <  SRR4302535.671381/94‑1 (MQ=60)

                                                        GATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACG                                      >  SRR4302535.3404929/1‑94 (MQ=60)

                                                         ATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGT                                     >  SRR4302535.4207894/1‑94 (MQ=60)

                                                             ATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAT                                 >  SRR4302535.514656/1‑94 (MQ=60)

                                                             ATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAT                                 <  SRR4302535.4334481/94‑1 (MQ=60)

                                                             ATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAT                                 <  SRR4302535.2578980/94‑1 (MQ=60)

                                                             ATTCCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAT                                 >  SRR4302535.3103557/1‑94 (MQ=60)

                                                                CCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTG                              <  SRR4302535.43325/94‑1 (MQ=60)

                                                                CCTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTG                              <  SRR4302535.1538311/94‑1 (MQ=60)

                                                                 CTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGT                             >  SRR4302535.3886235/1‑94 (MQ=60)

                                                                 CTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGT                             >  SRR4302535.2594679/1‑94 (MQ=60)

                                                                 CTCTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGT                             >  SRR4302535.4123007/1‑94 (MQ=60)

                                                                   CTCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTA                           >  SRR4302535.3278218/1‑94 (MQ=60)

                                                                    TCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAT                          >  SRR4302535.2625312/1‑94 (MQ=60)

                                                                    TCCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAT                          >  SRR4302535.724295/1‑94 (MQ=60)

                                                                     CCCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATT                         <  SRR4302535.2352315/94‑1 (MQ=60)

                                                                      CCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTG                        >  SRR4302535.1568151/1‑94 (MQ=60)

                                                                      CCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTG                        <  SRR4302535.2799806/94‑1 (MQ=60)

                                                                      CCGCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTG                        >  SRR4302535.4566586/1‑94 (MQ=60)

                                                                        GCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGAT                      <  SRR4302535.3045727/94‑1 (MQ=60)

                                                                        GCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGAT                      >  SRR4302535.2267764/1‑94 (MQ=60)

                                                                        GCATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGAT                      >  SRR4302535.2267766/1‑94 (MQ=60)

                                                                         CATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATG                     <  SRR4302535.1702918/94‑1 (MQ=60)

                                                                         CATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATG                     >  SRR4302535.3181536/1‑94 (MQ=60)

                                                                         CATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGATATTGATT                     >  SRR4302535.3473030/1‑94 (MQ=60)

                                                                          ATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGT                    <  SRR4302535.475152/94‑1 (MQ=60)

                                                                          ATTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGT                    >  SRR4302535.705495/1‑94 (MQ=60)

                                                                           TTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTT                   <  SRR4302535.3074684/94‑1 (MQ=60)

                                                                           TTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTT                   <  SRR4302535.3597886/94‑1 (MQ=60)

                                                                           TTTATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTT                   <  SRR4302535.4044926/94‑1 (MQ=60)

                                                                             TATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGG                 >  SRR4302535.4317556/1‑94 (MQ=60)

                                                                             TATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGG                 <  SRR4302535.2811154/94‑1 (MQ=60)

                                                                              ATGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGT                >  SRR4302535.4171675/1‑94 (MQ=60)

                                                                               TGGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTA               >  SRR4302535.3049101/1‑94 (MQ=60)

                                                                                GGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTAT              <  SRR4302535.4359765/94‑1 (MQ=60)

                                                                                GGGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTAT              <  SRR4302535.2470788/94‑1 (MQ=60)

                                                                                 GGAATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATT             <  SRR4302535.715035/94‑1 (MQ=60)

                                                                                   AATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTAC           <  SRR4302535.4436748/94‑1 (MQ=60)

                                                                                   AATGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTAC           >  SRR4302535.2760380/1‑94 (MQ=60)

                                                                                     TGCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTC         <  SRR4302535.293943/94‑1 (MQ=60)

                                                                                      GCGTAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCG        <  SRR4302535.3637115/94‑1 (MQ=60)

                                                                                        GTAGCGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTG      <  SRR4302535.1480410/94‑1 (MQ=60)

                                                                                         TAGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGG     <  SRR4302535.2169174/94‑1 (MQ=60)

                                                                                          AGCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGC    >  SRR4302535.37291/1‑94 (MQ=60)

                                                                                           GCGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCC   <  SRR4302535.599693/94‑1 (MQ=60)

                                                                                            CGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCG  >  SRR4302535.2191552/1‑94 (MQ=60)

CTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCG  >  NC_000913/3851418‑3851603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`