Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,857,508	T→C	E79G (GAA→GGA)	yidK ←	putative cotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,857,508	0	T	C	99.6%	696.0 / ‑4.7	223	E79G (GAA→GGA)	yidK	putative cotransporter
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (121/101); total (122/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

Only 100 of 230 total aligned reads displayed.

AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAA  >  NC_000913/3857418‑3857597

aGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTccc                                                                                         >  2:3478484/1‑93 (MQ=255)

aGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTccc                                                                                         >  2:1689018/1‑93 (MQ=255)

 gAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTcccc                                                                                        <  2:1661293/93‑1 (MQ=255)

  aaaaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCa                                                                                       >  2:4476225/1‑93 (MQ=255)

    aaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGc                                                                                     >  1:1004715/1‑93 (MQ=255)

    aaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGc                                                                                     >  2:2685130/1‑93 (MQ=255)

     aTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGcc                                                                                    >  1:3098505/1‑93 (MQ=255)

      tCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGccc                                                                                   >  1:2151122/1‑93 (MQ=255)

        ggggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCAt                                                                                 >  1:658241/1‑93 (MQ=255)

        ggggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCAt                                                                                 <  1:3373522/93‑1 (MQ=255)

           gATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAc                                                                              <  2:3689560/93‑1 (MQ=255)

             tggtggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCg                                                                            >  1:3087573/1‑93 (MQ=255)

               gtggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGAc                                                                          >  1:2059568/1‑93 (MQ=255)

                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  1:3514045/93‑1 (MQ=255)

                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  2:1379366/93‑1 (MQ=255)

                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  2:4002050/93‑1 (MQ=255)

                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  1:1423851/93‑1 (MQ=255)

                 ggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAt                                                                        <  1:1832419/93‑1 (MQ=255)

                 ggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAt                                                                        >  2:2044387/1‑93 (MQ=255)

                    aaTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATGACCGACATGcc                                                                     >  1:4213119/1‑93 (MQ=255)

                    aaTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGcc                                                                     >  2:2179568/1‑93 (MQ=255)

                     aTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCg                                                                    >  2:2422075/1‑93 (MQ=255)

                      tGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGc                                                                   >  1:866405/1‑93 (MQ=255)

                       gCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCt                                                                  >  2:1275378/1‑93 (MQ=255)

                         cgcgCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCttt                                                                <  2:3227078/93‑1 (MQ=255)

                           cgcTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTg                                                              <  1:3371548/93‑1 (MQ=255)

                             cTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTa                                                            <  2:2164979/93‑1 (MQ=255)

                             cTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTa                                                            <  2:1623203/93‑1 (MQ=255)

                              ttCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAg                                                           >  1:3045542/1‑93 (MQ=255)

                                  gATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCt                                                       >  2:915167/1‑93 (MQ=255)

                                    tAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGc                                                     >  2:1586534/1‑93 (MQ=255)

                                      gcgcGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGccc                                                   <  1:2272712/93‑1 (MQ=255)

                                        gcgGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGTCTGCCCgg                                                 >  1:1220799/1‑93 (MQ=255)

                                           gTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaaa                                              <  1:4478960/93‑1 (MQ=255)

                                            tAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAg                                             <  1:1000592/93‑1 (MQ=255)

                                             aaaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACGTGCCGCTTTTGTAGGACTGCCCGGAAAgg                                            >  2:1533727/1‑93 (MQ=255)

                                             aaaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAgg                                            >  2:2281458/1‑93 (MQ=255)

                                             aaaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAgg                                            >  2:4169260/1‑93 (MQ=255)

                                             aaaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAgg                                            >  2:4210806/1‑93 (MQ=255)

                                              aaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGc                                           >  1:4064979/1‑93 (MQ=255)

                                              aaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGc                                           <  1:1662580/93‑1 (MQ=255)

                                               aaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGcc                                          >  2:486229/1‑93 (MQ=255)

                                               aaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGcc                                          <  1:3143322/93‑1 (MQ=255)

                                                aaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCg                                         >  1:4568093/1‑93 (MQ=255)

                                                aaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCg                                         >  1:2561434/1‑93 (MQ=255)

                                                  gATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAc                                       >  1:604887/1‑93 (MQ=255)

                                                  aaTTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAc                                       <  2:3089266/92‑1 (MQ=255)

                                                   aTTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAcc                                      <  2:3747564/93‑1 (MQ=255)

                                                    ttAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCCGCCCATCACCGACATGCAGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCa                                     >  1:2100422/1‑93 (MQ=255)

                                                    ttAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCa                                     <  1:1938035/93‑1 (MQ=255)

                                                     tAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAg                                    <  2:2859294/93‑1 (MQ=255)

                                                      aGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgt                                   >  1:3773266/1‑93 (MQ=255)

                                                       gcgcGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgtt                                  >  1:4670129/1‑93 (MQ=255)

                                                         gcgAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgttgt                                >  1:2038400/1‑93 (MQ=255)

                                                          cgAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgttgtt                               <  2:3171027/93‑1 (MQ=255)

                                                           gAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTc                              <  1:2173105/93‑1 (MQ=255)

                                                            aGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTcc                             <  1:3607000/93‑1 (MQ=255)

                                                            aGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTcc                             >  2:2486960/1‑93 (MQ=255)

                                                             ggAAGATCAGCGTAACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCg                            >  2:384043/1‑93 (MQ=255)

                                                              gAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           >  1:2456081/1‑93 (MQ=255)

                                                              gAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           >  1:4528115/1‑93 (MQ=255)

                                                              gAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           <  1:4299708/93‑1 (MQ=255)

                                                               aaGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTg                          <  1:1118334/93‑1 (MQ=255)

                                                                 gATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGa                        >  2:140980/1‑93 (MQ=255)

                                                                  aTCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGaa                       >  2:1466219/1‑93 (MQ=255)

                                                                   tCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGaaa                      <  1:2879871/93‑1 (MQ=255)

                                                                   tCAGCATCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGaaa                      <  2:4312215/93‑1 (MQ=255)

                                                                     aGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAgg                    >  2:948744/1‑93 (MQ=255)

                                                                     aGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAgg                    <  2:1857318/93‑1 (MQ=255)

                                                                     aGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAgg                    >  2:1007729/1‑93 (MQ=255)

                                                                      gCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggt                   >  2:2607610/1‑93 (MQ=255)

                                                                      gCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggt                   >  1:1506154/1‑93 (MQ=255)

                                                                      gCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggt                   >  1:3905845/1‑93 (MQ=255)

                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 >  1:1333322/1‑93 (MQ=255)

                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 >  2:74922/1‑93 (MQ=255)

                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 <  2:4351405/93‑1 (MQ=255)

                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 >  1:3688849/1‑93 (MQ=255)

                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 >  2:3505257/1‑93 (MQ=255)

                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTACGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 <  1:2516157/93‑1 (MQ=255)

                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCCACATGCCGCTTTTGTAGGACTGCCCGGGAACGCCGACCAGGTGGTCCGTGGAAAggttg                 >  1:3496112/1‑93 (MQ=255)

                                                                        gTCACCGCTCAAGTCGCTCCCCAGCCCATCCGCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 <  1:1746345/93‑1 (MQ=255)

                                                                         tCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttgg                >  1:3984409/1‑93 (MQ=255)

                                                                          cACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGCAAggctggt               >  2:3436928/1‑93 (MQ=255)

                                                                            ccGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTa             >  2:2373833/1‑93 (MQ=255)

                                                                            ccGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTa             <  1:1550071/93‑1 (MQ=255)

                                                                             cGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAg            >  2:627778/1‑93 (MQ=255)

                                                                              gCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGc           >  1:2994908/1‑93 (MQ=255)

                                                                               cTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCa          <  1:258707/93‑1 (MQ=255)

                                                                                tGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAt         >  2:289627/1‑93 (MQ=255)

                                                                                tGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAt         <  1:4306469/93‑1 (MQ=255)

                                                                                tGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAt         <  2:4565694/93‑1 (MQ=255)

                                                                                 gAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAtt        <  2:85419/93‑1 (MQ=255)

                                                                                    gTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAc     >  1:1353385/1‑93 (MQ=255)

                                                                                    gTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGTAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAc     <  2:2649901/93‑1 (MQ=255)

                                                                                    gTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAc     >  1:1867707/1‑93 (MQ=255)

                                                                                    gTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAc     <  1:797927/93‑1 (MQ=255)

                                                                                    gTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAc     >  2:3270175/1‑93 (MQ=255)

                                                                                      cACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGa   <  1:3845702/93‑1 (MQ=255)

                                                                                      cACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGa   <  2:3387807/93‑1 (MQ=255)

                                                                                       aCTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCAGTGGAAAGGTTGGTTAGCATTAACGaa  <  2:1130823/93‑1 (MQ=255)

AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAA  >  NC_000913/3857418‑3857597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  >  NC_000913/3857415‑3857600

TCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTC                                                                                               <  SRR4302535.3242068/94‑1 (MQ=60)

 CCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCC                                                                                              >  SRR4302535.2495023/1‑94 (MQ=60)

 CCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCC                                                                                              >  SRR4302535.2366794/1‑94 (MQ=60)

 CCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCC                                                                                              >  SRR4302535.1719013/1‑94 (MQ=60)

    GAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑A                                                                                          <  SRR4302535.4671164/94‑1 (MQ=60)

     AAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AG                                                                                         <  SRR4302535.4333924/94‑1 (MQ=60)

     AAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AG                                                                                         <  SRR4302535.81298/94‑1 (MQ=60)

      AAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGC                                                                                        >  SRR4302535.1343210/1‑94 (MQ=60)

      AAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGC                                                                                        >  SRR4302535.1011852/1‑94 (MQ=60)

       AATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCC                                                                                       >  SRR4302535.3120077/1‑94 (MQ=60)

        ATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCC                                                                                      >  SRR4302535.2166587/1‑94 (MQ=60)

          CGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCAT                                                                                    >  SRR4302535.662837/1‑94 (MQ=60)

           GGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATC                                                                                   >  SRR4302535.3200768/1‑94 (MQ=60)

           GGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATC                                                                                   <  SRR4302535.3396985/94‑1 (MQ=60)

              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCCCCGCTGAAGTCACTCCCC‑AGCCCATCACC                                                                                <  SRR4302535.598409/94‑1 (MQ=60)

              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACC                                                                                >  SRR4302535.4638223/1‑94 (MQ=60)

               ATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCG                                                                               >  SRR4302535.3109078/1‑94 (MQ=60)

               ATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCG                                                                               >  SRR4302535.4379717/1‑94 (MQ=60)

                 GGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGAC                                                                             >  SRR4302535.2074407/1‑94 (MQ=60)

                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACAT                                                                           <  SRR4302535.3064647/94‑1 (MQ=60)

                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACAT                                                                           <  SRR4302535.1434019/94‑1 (MQ=60)

                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACAT                                                                           <  SRR4302535.3538464/94‑1 (MQ=60)

                    GGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATG                                                                          >  SRR4302535.141741/1‑94 (MQ=60)

                    GGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATG                                                                          <  SRR4302535.1845642/94‑1 (MQ=60)

                     GCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGC                                                                         >  SRR4302535.1529668/1‑94 (MQ=60)

                      CAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATGACCGACATGCC                                                                        >  SRR4302535.4241953/1‑94 (MQ=60)

                      CAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCC                                                                        >  SRR4302535.3138392/1‑94 (MQ=60)

                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCG                                                                       >  SRR4302535.1627569/1‑94 (MQ=60)

                        ATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGC                                                                      >  SRR4302535.872532/1‑94 (MQ=60)

                             GCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTG                                                                 <  SRR4302535.672321/94‑1 (MQ=60)

                             GCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTG                                                                 <  SRR4302535.2582997/94‑1 (MQ=60)

                              CGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGT                                                                <  SRR4302535.3395005/94‑1 (MQ=60)

                              CGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGA                                                                >  SRR4302535.2069463/1‑94 (MQ=60)

                               GCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTA                                                               >  SRR4302535.4548968/1‑94 (MQ=60)

                               GCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTA                                                               >  SRR4302535.148231/1‑94 (MQ=60)

                                CTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAG                                                              >  SRR4302535.3066876/1‑94 (MQ=60)

                                CTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAG                                                              >  SRR4302535.2032788/1‑94 (MQ=60)

                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                          <  SRR4302535.1752810/94‑1 (MQ=60)

                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                          >  SRR4302535.3511663/1‑94 (MQ=60)

                                    AGATAGCGCGCTAAAAAGATTAGCGCTAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCTCCGACATGCCGCTTTTGTAGGCCT                                                          <  SRR4302535.650558/94‑1 (MQ=60)

                                     GATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTG                                                         <  SRR4302535.689451/94‑1 (MQ=60)

                                      ATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGC                                                        >  SRR4302535.1870694/1‑94 (MQ=60)

                                         GCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCG                                                     <  SRR4302535.408754/94‑1 (MQ=60)

                                         GCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCG                                                     <  SRR4302535.2288925/94‑1 (MQ=60)

                                          CGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGTCTGCCCGG                                                    >  SRR4302535.1229548/1‑94 (MQ=60)

                                           GCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGA                                                   >  SRR4302535.1488388/1‑94 (MQ=60)

                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302535.3853820/94‑1 (MQ=60)

                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302535.4509711/94‑1 (MQ=60)

                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302535.991615/94‑1 (MQ=60)

                                               TAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGG                                               <  SRR4302535.1007703/94‑1 (MQ=60)

                                               TAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGG                                               >  SRR4302535.3101227/1‑94 (MQ=60)

                                                AAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGC                                              >  SRR4302535.4092795/1‑94 (MQ=60)

                                                 AAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCC                                             <  SRR4302535.1674592/94‑1 (MQ=60)

                                                 AAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCC                                             <  SRR4302535.2284330/94‑1 (MQ=60)

                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                            <  SRR4302535.3165189/94‑1 (MQ=60)

                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                            <  SRR4302535.2169167/94‑1 (MQ=60)

                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                            >  SRR4302535.2579876/1‑94 (MQ=60)

                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                            >  SRR4302535.4599249/1‑94 (MQ=60)

                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AACCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                            <  SRR4302535.3649487/94‑1 (MQ=60)

                                                    AGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAC                                          >  SRR4302535.609117/1‑94 (MQ=60)

                                                    AGATTAGCGCGAGGAAGATCAGCGTCACCCCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGACTGCCCGGACAAGCCGAC                                          >  SRR4302535.2040539/1‑94 (MQ=60)

                                                     GATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACC                                         >  SRR4302535.2514067/1‑94 (MQ=60)

                                                      ATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑CGCCCATCACCGACATGCAGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCA                                        >  SRR4302535.2115569/1‑94 (MQ=60)

                                                      ATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGGCCGGAAAGGCCGACCA                                        >  SRR4302535.2115568/1‑94 (MQ=60)

                                                       TTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAG                                       <  SRR4302535.1951988/94‑1 (MQ=60)

                                                        TAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGT                                      >  SRR4302535.3799292/1‑94 (MQ=60)

                                                        TAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGT                                      <  SRR4302535.3914655/94‑1 (MQ=60)

                                                         AGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTT                                     >  SRR4302535.4701676/1‑94 (MQ=60)

                                                           CGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGT                                   >  SRR4302535.2053079/1‑94 (MQ=60)

                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                <  SRR4302535.3938801/94‑1 (MQ=60)

                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                >  SRR4302535.3216624/1‑94 (MQ=60)

                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                <  SRR4302535.1009919/94‑1 (MQ=60)

                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                <  SRR4302535.2188650/94‑1 (MQ=60)

                                                               AGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCG                               >  SRR4302535.1012944/1‑94 (MQ=60)

                                                               AGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCG                               <  SRR4302535.3632020/94‑1 (MQ=60)

                                                                GGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGT                              >  SRR4302535.2473789/1‑94 (MQ=60)

                                                                GGAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGT                              >  SRR4302535.4559125/1‑94 (MQ=60)

                                                                 GAAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTG                             <  SRR4302535.4329131/94‑1 (MQ=60)

                                                                  AAGATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGG                            <  SRR4302535.1126312/94‑1 (MQ=60)

                                                                    GATCAGCGTCACCGCTGAAGTCCCTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAA                          <  SRR4302535.3396580/94‑1 (MQ=60)

                                                                     ATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAA                         >  SRR4302535.3068742/1‑94 (MQ=60)

                                                                     ATCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAA                         >  SRR4302535.3527343/1‑94 (MQ=60)

                                                                      TCAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAG                        <  SRR4302535.2900260/94‑1 (MQ=60)

                                                                      ACAGCGTCACCGCTGAAGTCCCTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAG                        <  SRR4302535.3727016/94‑1 (MQ=60)

                                                                       CAGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGG                       >  SRR4302535.12344/1‑94 (MQ=60)

                                                                        AGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGCAAGGT                      >  SRR4302535.1751308/1‑94 (MQ=60)

                                                                        AGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGT                      >  SRR4302535.3932605/1‑94 (MQ=60)

                                                                        AGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGT                      >  SRR4302535.1516983/1‑94 (MQ=60)

                                                                        AGCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGT                      >  SRR4302535.212869/1‑94 (MQ=60)

                                                                         GCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTT                     <  SRR4302535.4587843/94‑1 (MQ=60)

                                                                         GCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTT                     <  SRR4302535.1929825/94‑1 (MQ=60)

                                                                         GCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTT                     <  SRR4302535.4046084/94‑1 (MQ=60)

                                                                         GCGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTT                     <  SRR4302535.283851/94‑1 (MQ=60)

                                                                          CGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                    >  SRR4302535.3714377/1‑94 (MQ=60)

                                                                          CGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                    >  SRR4302535.1342831/1‑94 (MQ=60)

                                                                          CGTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCCACATGCCGCTTTTGTAGGACTGCCCGGGAACGCCGACCAGGTGGTCCGTGGAAAGGTTG                    >  SRR4302535.3520409/1‑94 (MQ=60)

                                                                           GTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   >  SRR4302535.4011633/1‑94 (MQ=60)

                                                                           GTCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTACGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302535.2534293/94‑1 (MQ=60)

                                                                           GTCACCGCTCAAGTCGCTCCCC‑AGCCCATCCGCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302535.1758979/94‑1 (MQ=60)

                                                                            TCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGT                  >  SRR4302535.4236791/1‑94 (MQ=60)

                                                                            TCACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGT                  >  SRR4302535.1778977/1‑94 (MQ=60)

                                                                             CACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTT                 >  SRR4302535.3110073/1‑94 (MQ=60)

                                                                              ACCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTA                <  SRR4302535.4291330/94‑1 (MQ=60)

                                                                               CCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG               >  SRR4302535.2557062/1‑94 (MQ=60)

                                                                               CCGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG               <  SRR4302535.1561237/94‑1 (MQ=60)

                                                                                CGCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGC              >  SRR4302535.3015904/1‑94 (MQ=60)

                                                                                 GCTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCA             >  SRR4302535.3561618/1‑94 (MQ=60)

                                                                                  CTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            >  SRR4302535.2374048/1‑94 (MQ=60)

                                                                                  CTGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            <  SRR4302535.260497/94‑1 (MQ=60)

                                                                                   TGAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATT           <  SRR4302535.4335933/94‑1 (MQ=60)

                                                                                    GAAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTA          <  SRR4302535.2939852/94‑1 (MQ=60)

                                                                                     AAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA         <  SRR4302535.2134809/94‑1 (MQ=60)

                                                                                     AAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA         <  SRR4302535.2399968/94‑1 (MQ=60)

                                                                                     AAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA         >  SRR4302535.2653374/1‑94 (MQ=60)

                                                                                     AAGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA         >  SRR4302535.1303967/1‑94 (MQ=60)

                                                                                      AGTCACTTCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC        >  SRR4302535.1363069/1‑94 (MQ=60)

                                                                                      AGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC        >  SRR4302535.3815336/1‑94 (MQ=60)

                                                                                      AGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC        >  SRR4302535.1881169/1‑94 (MQ=60)

                                                                                      AGTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAACGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC        >  SRR4302535.4406288/1‑94 (MQ=60)

                                                                                       GTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACG       >  SRR4302535.3616461/1‑94 (MQ=60)

                                                                                       GTCACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACG       <  SRR4302535.803527/94‑1 (MQ=60)

                                                                                         CACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAA     <  SRR4302535.3872181/94‑1 (MQ=60)

                                                                                          ACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG    <  SRR4302535.1202406/94‑1 (MQ=60)

                                                                                          ACTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG    >  SRR4302535.3106532/1‑94 (MQ=60)

                                                                                           CTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGC   <  SRR4302535.824826/94‑1 (MQ=60)

                                                                                           CTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGC   >  SRR4302535.4587367/1‑94 (MQ=60)

                                                                                           CTCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGC   >  SRR4302535.4572447/1‑94 (MQ=60)

                                                                                            TCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  >  SRR4302535.546954/1‑94 (MQ=60)

                                                                                            TCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  <  SRR4302535.1298772/94‑1 (MQ=60)

                                                                                            TCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  <  SRR4302535.2323358/94‑1 (MQ=60)

                                                                                            TCCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  <  SRR4302535.3595984/94‑1 (MQ=60)

                                                                                             TCCCAAGCCCATCAACGACATGCCGCTTTAGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCATGGAAAGGTTGGTTAGCATTAACGAAGCG  <  SRR4302535.4712515/94‑1 (MQ=60)

TCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCC‑AGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  >  NC_000913/3857415‑3857600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`