Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,857,508 T→C E79G (GAA→GGA)  yidK ← putative cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,857,5080TC99.2% 1167.2 / ‑2.7 373E79G (GAA→GGA) yidKputative cotransporter
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (172/197);  total (173/200)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 395 total aligned reads displayed.

AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAA  >  NC_000913/3857418‑3857597
                                                                                          |                                                                                         
aGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGGCACCGCTGAAGTCACTccc                                                                                         >  2:7407462/1‑93 (MQ=255)
 gAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTcccc                                                                                        <  1:5651416/93‑1 (MQ=255)
 gAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTcccc                                                                                        <  1:1454021/93‑1 (MQ=255)
  aaaaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCa                                                                                       <  1:1327069/93‑1 (MQ=255)
   aaaTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAg                                                                                      >  2:334672/1‑93 (MQ=255)
     aTCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGcc                                                                                    >  1:6183795/1‑93 (MQ=255)
      tCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGccc                                                                                   <  1:5456216/93‑1 (MQ=255)
      tCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGccc                                                                                   >  2:2919394/1‑93 (MQ=255)
       cGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCa                                                                                  <  2:3541583/93‑1 (MQ=255)
       cGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCa                                                                                  >  2:370276/1‑93 (MQ=255)
        ggggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCAt                                                                                 <  2:7842926/93‑1 (MQ=255)
         gggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATc                                                                                >  2:1641443/1‑93 (MQ=255)
         gggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATc                                                                                >  2:4057424/1‑93 (MQ=255)
         gggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATc                                                                                <  1:3129258/93‑1 (MQ=255)
         gggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATc                                                                                >  2:4967151/1‑93 (MQ=255)
          ggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCa                                                                               >  1:7743581/1‑93 (MQ=255)
          ggATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCa                                                                               <  1:2558938/93‑1 (MQ=255)
           gATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAc                                                                              <  1:983811/93‑1 (MQ=255)
           gATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAc                                                                              <  1:5222627/93‑1 (MQ=255)
           gATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAc                                                                              <  1:7530972/93‑1 (MQ=255)
           gATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCAGAAGTCACTCCCCAGCCCATCAc                                                                              >  2:5392194/1‑93 (MQ=255)
             tggtggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCg                                                                            >  1:572680/1‑93 (MQ=255)
               gtggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGAc                                                                          >  2:4789385/1‑93 (MQ=255)
                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  1:3025464/93‑1 (MQ=255)
                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  2:1808044/93‑1 (MQ=255)
                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  1:3207276/93‑1 (MQ=255)
                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  1:1496257/93‑1 (MQ=255)
                tggCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACa                                                                         <  1:5857690/93‑1 (MQ=255)
                    aaTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGcc                                                                     >  2:4211161/1‑93 (MQ=255)
                    aaTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGcc                                                                     >  1:4963776/1‑93 (MQ=255)
                     aTGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCg                                                                    >  1:620071/1‑93 (MQ=255)
                      tGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGc                                                                   >  2:6941770/1‑93 (MQ=255)
                      tGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGc                                                                   <  1:6947764/93‑1 (MQ=255)
                         cgcgCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCttt                                                                <  1:3761196/93‑1 (MQ=255)
                          gcgcTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCtttt                                                               >  2:3791040/1‑93 (MQ=255)
                              ttCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAg                                                           >  2:2850240/1‑93 (MQ=255)
                              ttCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAg                                                           <  1:7353878/93‑1 (MQ=255)
                               tCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAgg                                                          <  2:5817165/93‑1 (MQ=255)
                                cAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGc                                                         >  1:2018405/1‑93 (MQ=255)
                                 aGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGcc                                                        >  1:1412704/1‑93 (MQ=255)
                                  gATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCt                                                       >  2:2548894/1‑93 (MQ=255)
                                  gATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCt                                                       >  1:1808064/1‑93 (MQ=255)
                                   aTAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTg                                                      >  1:2581288/1‑93 (MQ=255)
                                    tAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGc                                                     >  2:83618/1‑93 (MQ=255)
                                    tAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGc                                                     <  2:2156256/93‑1 (MQ=255)
                                     aGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGcc                                                    >  1:4799098/1‑93 (MQ=255)
                                      gcgcGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGccc                                                   <  1:7405596/93‑1 (MQ=255)
                                        gcgGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCgg                                                 >  1:116050/1‑93 (MQ=255)
                                        gcgGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCgg                                                 <  1:5983128/93‑1 (MQ=255)
                                          ggTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaa                                               >  2:5090050/1‑93 (MQ=255)
                                           gTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaaa                                              <  2:2481524/93‑1 (MQ=255)
                                           gTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaaa                                              <  1:3497454/93‑1 (MQ=255)
                                           gTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGaaa                                              <  2:1499190/93‑1 (MQ=255)
                                             aaaaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAgg                                            >  2:6041631/1‑93 (MQ=255)
                                               aaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGcc                                          <  1:3408242/93‑1 (MQ=255)
                                               aaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGcc                                          >  2:1580145/1‑93 (MQ=255)
                                               aaaGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGcc                                          <  2:6338445/93‑1 (MQ=255)
                                                    ttAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCa                                     >  1:3922735/1‑93 (MQ=255)
                                                     tAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAg                                    <  1:3292555/93‑1 (MQ=255)
                                                        cgcgAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgttg                                 <  1:6404525/93‑1 (MQ=255)
                                                          cgAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgttgtt                               >  1:279859/1‑93 (MQ=255)
                                                          cgAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAgttgtt                               >  2:1295619/1‑93 (MQ=255)
                                                           gAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTc                              >  2:2734491/1‑93 (MQ=255)
                                                            aGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTcc                             <  2:108956/93‑1 (MQ=255)
                                                            aGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTcc                             >  2:7754358/1‑93 (MQ=255)
                                                            aGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTcc                             <  1:2332159/93‑1 (MQ=255)
                                                              gAAGATTAGCGTCACCGCTGAAGTCACTCCCCAGCCCATTACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCCGTTGTTCCgg                           >  2:2024259/1‑92 (MQ=255)
                                                              gAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           <  2:6707901/93‑1 (MQ=255)
                                                              gAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           >  2:7215674/1‑93 (MQ=255)
                                                              gAAGATCAGCGTCACCGCTGAAGTCACCCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGt                           <  1:2933619/93‑1 (MQ=255)
                                                                aGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTgg                         <  1:167970/93‑1 (MQ=255)
                                                                 gATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGa                        >  1:4683642/1‑93 (MQ=255)
                                                                 gATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGa                        >  2:3165820/1‑93 (MQ=255)
                                                                  aTCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGaa                       >  1:2492729/1‑93 (MQ=255)
                                                                   tCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGaaa                      >  2:451988/1‑93 (MQ=255)
                                                                   tCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGaaa                      <  2:7674560/93‑1 (MQ=255)
                                                                    cAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAg                     <  2:3906870/93‑1 (MQ=255)
                                                                    cAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAg                     <  2:4448401/93‑1 (MQ=255)
                                                                     aGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAgg                    <  2:1940676/93‑1 (MQ=255)
                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 <  1:4324291/93‑1 (MQ=255)
                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 <  1:4416453/93‑1 (MQ=255)
                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 <  1:5033471/93‑1 (MQ=255)
                                                                        gTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggttg                 >  1:7813217/1‑93 (MQ=255)
                                                                           aCCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAggctggtt              >  2:4557554/1‑93 (MQ=255)
                                                                            ccGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTa             <  1:6800480/93‑1 (MQ=255)
                                                                            ccGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTa             <  2:232072/93‑1 (MQ=255)
                                                                            ccGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGGTGTTCCGTGGAAAGGTTGGTTa             >  2:5891660/1‑93 (MQ=255)
                                                                               cTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCa          <  1:4131884/93‑1 (MQ=255)
                                                                                tGAAGTCACTCCCCAGCCCATCAGCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAt         <  1:837973/93‑1 (MQ=255)
                                                                                tGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAt         >  2:5216515/1‑93 (MQ=255)
                                                                                tGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAt         >  1:614588/1‑93 (MQ=255)
                                                                                 gAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAtt        <  1:6500130/93‑1 (MQ=255)
                                                                                  aaGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTa       <  1:2226039/93‑1 (MQ=255)
                                                                                   aGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTaa      <  2:5562852/93‑1 (MQ=255)
                                                                                   aGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTaa      >  1:7122681/1‑93 (MQ=255)
                                                                                   aGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTaa      <  1:102477/93‑1 (MQ=255)
                                                                                    gTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAc     >  2:5686564/1‑93 (MQ=255)
                                                                                    gTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAc     <  1:186206/93‑1 (MQ=255)
                                                                                      cACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGa   <  2:7530405/93‑1 (MQ=255)
                                                                                       aCTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGaa  <  2:4328171/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAA  >  NC_000913/3857418‑3857597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 216 total aligned reads displayed.
TCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  >  NC_000913/3857415‑3857600
                                                                                             |                                                                                            
TCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTC                                                                                              <  SRR4302543.4186554/94‑1 (MQ=60)
 CCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCC                                                                                             <  SRR4302543.1274186/94‑1 (MQ=60)
  CAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCC                                                                                            >  SRR4302543.7977429/1‑94 (MQ=60)
  CAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCC                                                                                            <  SRR4302543.1947332/94‑1 (MQ=60)
   AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC                                                                                           <  SRR4302543.2407692/94‑1 (MQ=60)
   AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC                                                                                           <  SRR4302543.2098763/94‑1 (MQ=60)
   AGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCC                                                                                           >  SRR4302543.7901960/1‑94 (MQ=60)
    GAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCA                                                                                          <  SRR4302543.5702937/94‑1 (MQ=60)
    GAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCA                                                                                          <  SRR4302543.1467144/94‑1 (MQ=60)
     AAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAG                                                                                         <  SRR4302543.7467231/94‑1 (MQ=60)
     AAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAG                                                                                         <  SRR4302543.1338850/94‑1 (MQ=60)
     AAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGAAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAG                                                                                         >  SRR4302543.3179098/1‑94 (MQ=60)
       AATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCC                                                                                       <  SRR4302543.7495812/94‑1 (MQ=60)
        ATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCC                                                                                      >  SRR4302543.7604637/1‑94 (MQ=60)
         TCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCA                                                                                     <  SRR4302543.5505684/94‑1 (MQ=60)
         TCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCA                                                                                     <  SRR4302543.2726738/94‑1 (MQ=60)
          CGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCAT                                                                                    >  SRR4302543.1379351/1‑94 (MQ=60)
           GGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATC                                                                                   >  SRR4302543.7446507/1‑94 (MQ=60)
           GGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATC                                                                                   >  SRR4302543.3379411/1‑94 (MQ=60)
            GGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCA                                                                                  >  SRR4302543.7816723/1‑94 (MQ=60)
            GGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCA                                                                                  <  SRR4302543.3157405/94‑1 (MQ=60)
            GGGATGGTGGCAATGCCGCGCTTAAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCAATCCCCAGCCCATCA                                                                                  <  SRR4302543.3157404/94‑1 (MQ=60)
             GGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAC                                                                                 <  SRR4302543.5143928/94‑1 (MQ=60)
             GGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCAC                                                                                 <  SRR4302543.2582322/94‑1 (MQ=60)
              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACC                                                                                <  SRR4302543.1522542/94‑1 (MQ=60)
              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACC                                                                                <  SRR4302543.7602798/94‑1 (MQ=60)
              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACC                                                                                <  SRR4302543.5269655/94‑1 (MQ=60)
              GATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACC                                                                                <  SRR4302543.745519/94‑1 (MQ=60)
               ATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCG                                                                               >  SRR4302543.6022856/1‑94 (MQ=60)
               ATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCG                                                                               >  SRR4302543.577923/1‑94 (MQ=60)
                TGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGA                                                                              <  SRR4302543.3722724/94‑1 (MQ=60)
                 GGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGAC                                                                             >  SRR4302543.7059533/1‑94 (MQ=60)
                 GGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATGAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTGCCCAGCCCATCACCGAC                                                                             <  SRR4302543.6888371/94‑1 (MQ=60)
                  GTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACA                                                                            >  SRR4302543.6500850/1‑94 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                           >  SRR4302543.109951/1‑94 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                           <  SRR4302543.5911013/94‑1 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                           <  SRR4302543.3236219/94‑1 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                           <  SRR4302543.3052802/94‑1 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                           <  SRR4302543.1493576/94‑1 (MQ=60)
                   TGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                           <  SRR4302543.1509722/94‑1 (MQ=60)
                   GGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACAT                                                                           <  SRR4302543.4244800/94‑1 (MQ=60)
                    TGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATG                                                                          <  SRR4302543.6480492/94‑1 (MQ=60)
                     GCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGC                                                                         <  SRR4302543.772799/94‑1 (MQ=60)
                      CAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCC                                                                        <  SRR4302543.1508463/94‑1 (MQ=60)
                      CAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCC                                                                        >  SRR4302543.5008962/1‑94 (MQ=60)
                      CAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCC                                                                        <  SRR4302543.2516634/94‑1 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCG                                                                       >  SRR4302543.625747/1‑94 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCG                                                                       <  SRR4302543.2588436/94‑1 (MQ=60)
                       AATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCCACATGCCG                                                                       >  SRR4302543.7871343/1‑94 (MQ=60)
                         TGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCT                                                                     <  SRR4302543.7013131/94‑1 (MQ=60)
                         TGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCT                                                                     >  SRR4302543.1350323/1‑94 (MQ=60)
                           CCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTT                                                                   >  SRR4302543.6251242/1‑94 (MQ=60)
                            CGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTT                                                                  <  SRR4302543.3166680/94‑1 (MQ=60)
                                CTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAG                                                              <  SRR4302543.307268/94‑1 (MQ=60)
                                CTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAG                                                              <  SRR4302543.3281853/94‑1 (MQ=60)
                                 TTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGG                                                             >  SRR4302543.1241894/1‑94 (MQ=60)
                                 TTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGG                                                             <  SRR4302543.7424617/94‑1 (MQ=60)
                                 TTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGG                                                             <  SRR4302543.7860202/94‑1 (MQ=60)
                                  TCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGC                                                            >  SRR4302543.2036540/1‑94 (MQ=60)
                                  TCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGC                                                            <  SRR4302543.6360265/94‑1 (MQ=60)
                                   CAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCTCAGCCCATCACCGACATGCCGCTTTTGTAGGCC                                                           >  SRR4302543.7086168/1‑94 (MQ=60)
                                   CAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCC                                                           >  SRR4302543.295134/1‑94 (MQ=60)
                                   CAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCC                                                           >  SRR4302543.1425477/1‑94 (MQ=60)
                                   CAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGACCATCACCGACATGCCGCTTTTGTAGGCC                                                           >  SRR4302543.2598999/1‑94 (MQ=60)
                                   CAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGATGACGTCACTCCCCAGCCCATCCCCGACATGCCGCTTTTGTAGGCC                                                           >  SRR4302543.4477972/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCTCTCACCGACATGCCGCTTTTGTAGGCCT                                                          >  SRR4302543.392301/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                          >  SRR4302543.3509950/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                          >  SRR4302543.1824249/1‑94 (MQ=60)
                                    AGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCT                                                          >  SRR4302543.181011/1‑94 (MQ=60)
                                     GATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCGCTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTG                                                         <  SRR4302543.4018064/94‑1 (MQ=60)
                                     GATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTG                                                         >  SRR4302543.2604787/1‑94 (MQ=60)
                                     GATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTG                                                         <  SRR4302543.6959528/94‑1 (MQ=60)
                                      ATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGC                                                        >  SRR4302543.963328/1‑94 (MQ=60)
                                       TAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCC                                                       >  SRR4302543.4842957/1‑94 (MQ=60)
                                        AGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCC                                                      <  SRR4302543.1236822/94‑1 (MQ=60)
                                        AGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACCCCCCACCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCC                                                      <  SRR4302543.2642735/94‑1 (MQ=60)
                                         GCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCG                                                     <  SRR4302543.1465771/94‑1 (MQ=60)
                                         GCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCG                                                     <  SRR4302543.7476668/94‑1 (MQ=60)
                                          CGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGG                                                    >  SRR4302543.117103/1‑94 (MQ=60)
                                          CGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGG                                                    >  SRR4302543.716605/1‑94 (MQ=60)
                                          CGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGG                                                    <  SRR4302543.3478784/94‑1 (MQ=60)
                                          CGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGG                                                    <  SRR4302543.3478785/94‑1 (MQ=60)
                                           GCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGA                                                   <  SRR4302543.6037955/94‑1 (MQ=60)
                                             GGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAA                                                 >  SRR4302543.2447692/1‑94 (MQ=60)
                                             GGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAA                                                 >  SRR4302543.6258989/1‑94 (MQ=60)
                                             GGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAA                                                 >  SRR4302543.5101410/1‑94 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302543.4694787/94‑1 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302543.6477113/94‑1 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302543.4030407/94‑1 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302543.5809178/94‑1 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                >  SRR4302543.1056010/1‑94 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302543.231422/94‑1 (MQ=60)
                                              GTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAG                                                <  SRR4302543.3528741/94‑1 (MQ=60)
                                               TAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGG                                               <  SRR4302543.5741555/94‑1 (MQ=60)
                                               TAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGG                                               <  SRR4302543.5741556/94‑1 (MQ=60)
                                                 AAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCC                                             >  SRR4302543.3387259/1‑94 (MQ=60)
                                                 AAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCC                                             >  SRR4302543.1492458/1‑94 (MQ=60)
                                                 AAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCC                                             <  SRR4302543.620922/94‑1 (MQ=60)
                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                            >  SRR4302543.253715/1‑94 (MQ=60)
                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                            <  SRR4302543.3438755/94‑1 (MQ=60)
                                                  AAAGATTAGCGCGAGGAAGATCAGCGTCACCGCGGAAGTCACTCCCTAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCG                                            <  SRR4302543.7700606/94‑1 (MQ=60)
                                                   AATATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGA                                           >  SRR4302543.1714926/1‑94 (MQ=60)
                                                   AAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGA                                           >  SRR4302543.2590607/1‑94 (MQ=60)
                                                   AAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGA                                           >  SRR4302543.405258/1‑94 (MQ=60)
                                                    AGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAC                                          <  SRR4302543.6922911/94‑1 (MQ=60)
                                                    AGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGAC                                          <  SRR4302543.2851669/94‑1 (MQ=60)
                                                     GATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACC                                         >  SRR4302543.7509551/1‑94 (MQ=60)
                                                      ATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCA                                        >  SRR4302543.3957353/1‑94 (MQ=60)
                                                       TTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAG                                       <  SRR4302543.6351250/94‑1 (MQ=60)
                                                        TAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGT                                      <  SRR4302543.3322265/94‑1 (MQ=60)
                                                         AGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTT                                     <  SRR4302543.3198479/94‑1 (MQ=60)
                                                         AGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTT                                     <  SRR4302543.4747329/94‑1 (MQ=60)
                                                         AGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTT                                     <  SRR4302543.7495385/94‑1 (MQ=60)
                                                           CGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTATGCCTGCCCGGAAAGGCCGACCAGTTGT                                   >  SRR4302543.5331600/1‑94 (MQ=60)
                                                           CGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGT                                   <  SRR4302543.6464838/94‑1 (MQ=60)
                                                            GCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTT                                  >  SRR4302543.282338/1‑94 (MQ=60)
                                                            GCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTT                                  <  SRR4302543.728616/94‑1 (MQ=60)
                                                            GCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTT                                  <  SRR4302543.3896519/94‑1 (MQ=60)
                                                            GCGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTT                                  <  SRR4302543.5012364/94‑1 (MQ=60)
                                                             CGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTC                                 >  SRR4302543.36319/1‑94 (MQ=60)
                                                             CGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTC                                 >  SRR4302543.488649/1‑94 (MQ=60)
                                                             CGAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTC                                 <  SRR4302543.3004815/94‑1 (MQ=60)
                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                >  SRR4302543.3319325/1‑94 (MQ=60)
                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                >  SRR4302543.4363659/1‑94 (MQ=60)
                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                >  SRR4302543.2712510/1‑94 (MQ=60)
                                                              GAGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCC                                <  SRR4302543.1872034/94‑1 (MQ=60)
                                                               AGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCG                               <  SRR4302543.1186167/94‑1 (MQ=60)
                                                               AGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCG                               >  SRR4302543.5021966/1‑94 (MQ=60)
                                                               AGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCG                               <  SRR4302543.2353325/94‑1 (MQ=60)
                                                               AGGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCG                               <  SRR4302543.6588439/94‑1 (MQ=60)
                                                                GGAAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGT                              <  SRR4302543.2225696/94‑1 (MQ=60)
                                                                  AAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGG                            >  SRR4302543.6693140/1‑94 (MQ=60)
                                                                  AAGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGG                            <  SRR4302543.1582306/94‑1 (MQ=60)
                                                                   AGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGA                           <  SRR4302543.169417/94‑1 (MQ=60)
                                                                   AGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGA                           >  SRR4302543.4726365/1‑94 (MQ=60)
                                                                   AGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGA                           >  SRR4302543.4726366/1‑94 (MQ=60)
                                                                   AGATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGA                           >  SRR4302543.3843092/1‑94 (MQ=60)
                                                                    GATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAA                          <  SRR4302543.4969328/94‑1 (MQ=60)
                                                                    GATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAA                          <  SRR4302543.2488692/94‑1 (MQ=60)
                                                                    GATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAA                          <  SRR4302543.6062460/94‑1 (MQ=60)
                                                                    GATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAA                          >  SRR4302543.2515331/1‑94 (MQ=60)
                                                                     ATCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAA                         >  SRR4302543.621016/1‑94 (MQ=60)
                                                                      TCAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAG                        <  SRR4302543.6021033/94‑1 (MQ=60)
                                                                       CAGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGG                       >  SRR4302543.894914/1‑94 (MQ=60)
                                                                        AGCGTCGCCGCTGAAGTCCCCCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGT                      <  SRR4302543.5678301/94‑1 (MQ=60)
                                                                        AGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGT                      >  SRR4302543.5011059/1‑94 (MQ=60)
                                                                        AGCGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGT                      <  SRR4302543.5678300/94‑1 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                    >  SRR4302543.7886848/1‑94 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                    <  SRR4302543.4820462/94‑1 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                    >  SRR4302543.1454471/1‑94 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                    <  SRR4302543.3529723/94‑1 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                    >  SRR4302543.7886847/1‑94 (MQ=60)
                                                                          CGTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTG                    <  SRR4302543.4442250/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   >  SRR4302543.7227022/1‑94 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302543.4456485/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302543.6245216/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302543.5079258/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302543.46938/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302543.6106300/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302543.3136072/94‑1 (MQ=60)
                                                                           GTCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302543.5558977/94‑1 (MQ=60)
                                                                           GGCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGG                   <  SRR4302543.2009882/94‑1 (MQ=60)
                                                                            TCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGT                  >  SRR4302543.5852959/1‑94 (MQ=60)
                                                                            TCACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGT                  >  SRR4302543.2309066/1‑94 (MQ=60)
                                                                             CACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTT                 <  SRR4302543.1675904/94‑1 (MQ=60)
                                                                              ACCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTA                >  SRR4302543.5736570/1‑94 (MQ=60)
                                                                               CCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG               <  SRR4302543.6218157/94‑1 (MQ=60)
                                                                               CCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG               >  SRR4302543.1375277/1‑94 (MQ=60)
                                                                               CCGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAG               <  SRR4302543.6864696/94‑1 (MQ=60)
                                                                                CGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGC              <  SRR4302543.4171336/94‑1 (MQ=60)
                                                                                CGCTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGC              >  SRR4302543.2016847/1‑94 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            <  SRR4302543.934307/94‑1 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            <  SRR4302543.1856166/94‑1 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            <  SRR4302543.7903232/94‑1 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            >  SRR4302543.6201564/1‑94 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            >  SRR4302543.1835637/1‑94 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            <  SRR4302543.4169155/94‑1 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            >  SRR4302543.620209/1‑94 (MQ=60)
                                                                                  CTGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCAT            <  SRR4302543.3048723/94‑1 (MQ=60)
                                                                                   TGAAGTCACTCCCCAGCCCATCAGCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATT           <  SRR4302543.845366/94‑1 (MQ=60)
                                                                                   TGAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATT           <  SRR4302543.4276131/94‑1 (MQ=60)
                                                                                    GAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTA          <  SRR4302543.6561367/94‑1 (MQ=60)
                                                                                    GAAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTA          <  SRR4302543.2976028/94‑1 (MQ=60)
                                                                                     AAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA         >  SRR4302543.7191873/1‑94 (MQ=60)
                                                                                     AAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA         <  SRR4302543.2246133/94‑1 (MQ=60)
                                                                                     AAGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGGAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAA         <  SRR4302543.2343445/94‑1 (MQ=60)
                                                                                      AGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC        <  SRR4302543.103425/94‑1 (MQ=60)
                                                                                      AGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC        <  SRR4302543.5953761/94‑1 (MQ=60)
                                                                                      AGTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAAC        <  SRR4302543.442207/94‑1 (MQ=60)
                                                                                       GTCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACG       <  SRR4302543.187787/94‑1 (MQ=60)
                                                                                       ATCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACG       <  SRR4302543.187788/94‑1 (MQ=60)
                                                                                        TCACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGA      >  SRR4302543.5335341/1‑94 (MQ=60)
                                                                                         CACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAA     >  SRR4302543.2959680/1‑94 (MQ=60)
                                                                                          ACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG    <  SRR4302543.5250557/94‑1 (MQ=60)
                                                                                          ACTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAG    >  SRR4302543.2017840/1‑94 (MQ=60)
                                                                                           CTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGC   >  SRR4302543.5815423/1‑94 (MQ=60)
                                                                                           CTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGC   >  SRR4302543.2864289/1‑94 (MQ=60)
                                                                                           CTCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGC   <  SRR4302543.7644908/94‑1 (MQ=60)
                                                                                            TCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  <  SRR4302543.5691378/94‑1 (MQ=60)
                                                                                            TCCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  <  SRR4302543.6281534/94‑1 (MQ=60)
                                                                                             |                                                                                            
TCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGTCACTTCCCAGCCCATCACCGACATGCCGCTTTTGTAGGCCTGCCCGGAAAGGCCGACCAGTTGTTCCGTGGAAAGGTTGGTTAGCATTAACGAAGCG  >  NC_000913/3857415‑3857600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑