Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,892,505 T→C G75G (GGT→GGC)  yieF → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,892,5050TC99.6% 785.7 / ‑6.1 252G75G (GGT→GGC) yieFhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (121/130);  minor base G (0/1);  total (121/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 271 total aligned reads displayed.

CATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACC  >  NC_000913/3892415‑3892594
                                                                                          |                                                                                         
cATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGgcgt                                                                                         >  1:2631336/1‑93 (MQ=255)
 aTTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGcgtg                                                                                        <  2:2300635/93‑1 (MQ=255)
  ttCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCgtgg                                                                                       <  2:2542501/93‑1 (MQ=255)
   tCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCgtggt                                                                                      <  2:2669521/93‑1 (MQ=255)
   tCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCgtggt                                                                                      >  2:2774460/1‑93 (MQ=255)
   tCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCgtggt                                                                                      <  2:3099420/93‑1 (MQ=255)
     ccTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGa                                                                                    <  1:246269/93‑1 (MQ=255)
     ccTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGa                                                                                    >  2:3691090/1‑93 (MQ=255)
     ccTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGa                                                                                    <  2:4446219/93‑1 (MQ=255)
     ccTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGa                                                                                    >  2:854390/1‑93 (MQ=255)
      cTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGAt                                                                                   >  2:3274059/1‑93 (MQ=255)
      cTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGAt                                                                                   >  1:3487746/1‑93 (MQ=255)
       ttGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATc                                                                                  >  1:1558329/1‑93 (MQ=255)
       ttGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATc                                                                                  >  2:3606994/1‑93 (MQ=255)
       ttGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATc                                                                                  <  2:2212734/93‑1 (MQ=255)
       ttGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATc                                                                                  >  2:2091793/1‑93 (MQ=255)
         gTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGt                                                                                <  2:1254912/93‑1 (MQ=255)
          tATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTc                                                                               <  2:1767195/93‑1 (MQ=255)
          tATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTc                                                                               >  2:1524902/1‑93 (MQ=255)
          tATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGAGTGGTGATCGTc                                                                               >  1:3254050/1‑93 (MQ=255)
            tGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCAc                                                                             <  1:1892233/93‑1 (MQ=255)
            tGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCAc                                                                             >  2:4287526/1‑93 (MQ=255)
             gACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACg                                                                            >  1:780928/1‑93 (MQ=255)
              aCGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGc                                                                           >  1:407415/1‑93 (MQ=255)
              aCGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGc                                                                           >  2:1359274/1‑93 (MQ=255)
                gatGACGTACACCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCg                                                                         <  2:3314655/91‑1 (MQ=255)
                 cTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCgg                                                                        <  1:1758143/93‑1 (MQ=255)
                  tGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGa                                                                       >  1:3671612/1‑93 (MQ=255)
                  tGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGa                                                                       <  1:3723868/93‑1 (MQ=255)
                   gACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGaa                                                                      <  1:3319315/93‑1 (MQ=255)
                   gACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGaa                                                                      <  1:1448711/93‑1 (MQ=255)
                   gACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGaa                                                                      >  1:4586961/1‑93 (MQ=255)
                    aCGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAat                                                                     <  1:975883/93‑1 (MQ=255)
                    aCGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAat                                                                     <  2:4329217/93‑1 (MQ=255)
                     cGTACAGCAGGAAGAAGGTTTTCCAGCAACGTTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAata                                                                    <  1:312568/93‑1 (MQ=255)
                      gTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAatat                                                                   <  1:2139406/93‑1 (MQ=255)
                        aCAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATaa                                                                 >  1:2583380/1‑93 (MQ=255)
                           gcagGAAGAAGGCTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGCGATCGGCATGCCGGACTATAactc                                                              >  2:2388703/1‑92 (MQ=255)
                            gagGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAactac                                                             <  2:3403343/92‑1 (MQ=255)
                            cagGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAactac                                                             <  2:2718050/93‑1 (MQ=255)
                             agGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAactact                                                            >  1:137786/1‑93 (MQ=255)
                              ggAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTc                                                           >  2:1315003/1‑93 (MQ=255)
                                aagaagGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCgg                                                         <  2:1086691/93‑1 (MQ=255)
                                 agaagGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGt                                                        >  2:665727/1‑93 (MQ=255)
                                  gaagGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTa                                                       >  2:3742229/1‑93 (MQ=255)
                                  gaagGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTa                                                       >  1:1104050/1‑93 (MQ=255)
                                    agGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTAcc                                                     <  1:13179/93‑1 (MQ=255)
                                     ggTTTTCAAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCg                                                    >  1:3977008/1‑93 (MQ=255)
                                      gTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCgg                                                   >  2:3836768/1‑93 (MQ=255)
                                      gTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCgg                                                   >  1:2972453/1‑93 (MQ=255)
                                         ttCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTg                                                <  2:2882449/93‑1 (MQ=255)
                                          tCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTgg                                               >  2:4191293/1‑93 (MQ=255)
                                           ccAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTggg                                              >  2:1046563/1‑93 (MQ=255)
                                           ccAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTggg                                              >  2:460792/1‑93 (MQ=255)
                                             aGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCt                                            >  1:1634319/1‑93 (MQ=255)
                                              gCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTg                                           >  2:1699844/1‑93 (MQ=255)
                                                aaCGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATTGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGaa                                         <  1:4312940/93‑1 (MQ=255)
                                                aaCGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGGGGGCTGaa                                         >  2:3188727/1‑93 (MQ=255)
                                                 aCGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGaaa                                        >  2:812926/1‑93 (MQ=255)
                                                 aCGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGaaa                                        >  1:1520923/1‑93 (MQ=255)
                                                  cGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGaaaa                                       <  2:4496648/93‑1 (MQ=255)
                                                  cGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGAGGTGATCGGCACGCCGGCATATAACTACTCGGTACCGGGTGGCCTGaaaa                                       >  1:3073311/1‑93 (MQ=255)
                                                   gggtGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGaaaaa                                      <  2:1337771/90‑1 (MQ=255)
                                                   ggTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGaaaaa                                      <  2:1430506/93‑1 (MQ=255)
                                                   ggTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGaaaaa                                      <  2:1620616/93‑1 (MQ=255)
                                                    gTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATTGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAAt                                     >  2:2971807/1‑93 (MQ=255)
                                                     ttGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATg                                    >  1:1419506/1‑93 (MQ=255)
                                                     ttGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATg                                    <  2:1942507/93‑1 (MQ=255)
                                                       gAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGcc                                  >  1:1748237/1‑93 (MQ=255)
                                                       gAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGcc                                  >  1:1693692/1‑93 (MQ=255)
                                                       gAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGcc                                  >  2:3024895/1‑93 (MQ=255)
                                                          gCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATc                               >  2:1804048/1‑93 (MQ=255)
                                                          gCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATc                               >  2:3563508/1‑93 (MQ=255)
                                                          gCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATc                               >  2:4137483/1‑93 (MQ=255)
                                                          gCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATc                               <  2:486425/93‑1 (MQ=255)
                                                           ctctGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCg                              >  1:4249598/1‑93 (MQ=255)
                                                            tctGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGa                             <  1:2159632/93‑1 (MQ=255)
                                                            tctGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGa                             >  2:1993436/1‑93 (MQ=255)
                                                               ggCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTg                          >  1:4624849/1‑93 (MQ=255)
                                                                gCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTgg                         >  2:3054339/1‑93 (MQ=255)
                                                                 cGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGc                        >  2:4040687/1‑93 (MQ=255)
                                                                   gAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCtt                      >  1:2345956/1‑93 (MQ=255)
                                                                    aaCAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCttt                     <  2:3625196/93‑1 (MQ=255)
                                                                    aaCAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTCGCttt                     >  1:334835/1‑93 (MQ=255)
                                                                     aCAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTc                    >  1:684330/1‑93 (MQ=255)
                                                                     aCAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTc                    >  2:2913251/1‑93 (MQ=255)
                                                                      cAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTcc                   >  1:615984/1‑93 (MQ=255)
                                                                         aTCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGc                >  1:2331819/1‑93 (MQ=255)
                                                                          tCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGcc               >  1:4155631/1‑93 (MQ=255)
                                                                           ccGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCt              <  1:3574969/93‑1 (MQ=255)
                                                                             gTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGc            >  1:2875841/1‑93 (MQ=255)
                                                                              tCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGcc           <  2:61037/93‑1 (MQ=255)
                                                                                  gCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGAt       <  1:2061349/93‑1 (MQ=255)
                                                                                   cTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATc      >  2:4008073/1‑93 (MQ=255)
                                                                                    tGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCa     >  1:1040200/1‑93 (MQ=255)
                                                                                    tGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCa     >  2:183500/1‑93 (MQ=255)
                                                                                     gACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCaa    <  2:3297366/93‑1 (MQ=255)
                                                                                      gcGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAAc   <  1:4432614/92‑1 (MQ=255)
                                                                                      aCGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAAc   <  2:4530551/93‑1 (MQ=255)
                                                                                       cGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAAcc  <  1:1990629/93‑1 (MQ=255)
                                                                                          |                                                                                         
CATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACC  >  NC_000913/3892415‑3892594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGC  >  NC_000913/3892412‑3892596
                                                                                             |                                                                                           
CGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGC                                                                                             >  SRR4302535.3865384/1‑94 (MQ=60)
  ACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGT                                                                                           >  SRR4302535.2650187/1‑94 (MQ=60)
  ACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGT                                                                                           <  SRR4302535.1143298/94‑1 (MQ=60)
  ACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGT                                                                                           <  SRR4302535.3355792/94‑1 (MQ=60)
   CATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTG                                                                                          >  SRR4302535.793442/1‑94 (MQ=60)
   CATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTG                                                                                          <  SRR4302535.3561661/94‑1 (MQ=60)
   CATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGCTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGGCGGCGTG                                                                                          >  SRR4302535.4103406/1‑94 (MQ=60)
      TCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTG                                                                                       <  SRR4302535.4301610/94‑1 (MQ=60)
      TCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTG                                                                                       <  SRR4302535.4251626/94‑1 (MQ=60)
      TCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTG                                                                                       >  SRR4302535.2990381/1‑94 (MQ=60)
        CCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGAT                                                                                     <  SRR4302535.247958/94‑1 (MQ=60)
        CCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGAT                                                                                     >  SRR4302535.3511959/1‑94 (MQ=60)
        CCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGAT                                                                                     <  SRR4302535.3634187/94‑1 (MQ=60)
         CTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATC                                                                                    >  SRR4302535.1569565/1‑94 (MQ=60)
         CTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATC                                                                                    >  SRR4302535.1709788/1‑94 (MQ=60)
            GTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGAGTGGTGATCGTC                                                                                 >  SRR4302535.3276659/1‑94 (MQ=60)
             TATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCA                                                                                <  SRR4302535.2094724/94‑1 (MQ=60)
              ATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCAC                                                                               >  SRR4302535.2826016/1‑94 (MQ=60)
               TGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACG                                                                              <  SRR4302535.1905859/94‑1 (MQ=60)
               TGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACG                                                                              >  SRR4302535.786411/1‑94 (MQ=60)
                GACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGC                                                                             >  SRR4302535.2014015/1‑94 (MQ=60)
                GACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGC                                                                             <  SRR4302535.2346077/94‑1 (MQ=60)
                GACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGC                                                                             >  SRR4302535.410270/1‑94 (MQ=60)
                GACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGCGGTGATCGTCACGC                                                                             >  SRR4302535.4046538/1‑94 (MQ=60)
                 ACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCC                                                                            <  SRR4302535.3385772/94‑1 (MQ=60)
                   GCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGG                                                                          <  SRR4302535.4627143/94‑1 (MQ=60)
                   GCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGG                                                                          >  SRR4302535.263849/1‑94 (MQ=60)
                    CTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGA                                                                         <  SRR4302535.1770851/94‑1 (MQ=60)
                    CTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGA                                                                         >  SRR4302535.1988634/1‑94 (MQ=60)
                    CTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGA                                                                         >  SRR4302535.3697040/1‑94 (MQ=60)
                     TGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAA                                                                        >  SRR4302535.4618197/1‑94 (MQ=60)
                     TGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAA                                                                        <  SRR4302535.3749664/94‑1 (MQ=60)
                      GACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAAT                                                                       <  SRR4302535.3342410/94‑1 (MQ=60)
                      GACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAAT                                                                       <  SRR4302535.392513/94‑1 (MQ=60)
                      GACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAAT                                                                       <  SRR4302535.1459067/94‑1 (MQ=60)
                       ACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATA                                                                      <  SRR4302535.982810/94‑1 (MQ=60)
                       ACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATA                                                                      <  SRR4302535.2090066/94‑1 (MQ=60)
                        CGTACAGCAGGAAGAAGGTTTTCCAGCAACGTTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATAT                                                                     <  SRR4302535.314732/94‑1 (MQ=60)
                        CGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATAT                                                                     <  SRR4302535.908737/94‑1 (MQ=60)
                        CGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATAT                                                                     >  SRR4302535.3210106/1‑94 (MQ=60)
                        CGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCATGGTGATCGTCACGCAGGAATAT                                                                     >  SRR4302535.1668969/1‑94 (MQ=60)
                         GTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATA                                                                    <  SRR4302535.2154814/94‑1 (MQ=60)
                         GTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATA                                                                    >  SRR4302535.1691376/1‑94 (MQ=60)
                         GTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATA                                                                    <  SRR4302535.331647/94‑1 (MQ=60)
                         GTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATA                                                                    >  SRR4302535.4632874/1‑94 (MQ=60)
                          TACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAA                                                                   >  SRR4302535.2601941/1‑94 (MQ=60)
                          TACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAA                                                                   <  SRR4302535.1620869/94‑1 (MQ=60)
                            CAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACT                                                                 <  SRR4302535.1067089/94‑1 (MQ=60)
                             AGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTA                                                                >  SRR4302535.186903/1‑94 (MQ=60)
                             AGCAGGAAGAAGGGTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACCGCGAGGTGCTCGTCCAGCCGGAATATAACTC                                                                >  SRR4302535.3105356/1‑94 (MQ=60)
                               CAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGTAACAGATCCGTCAGGCTGACGGCGTCGTGATCGTCATGCCGGAATATAACTACT                                                              >  SRR4302535.3058208/1‑94 (MQ=60)
                               CAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACT                                                              <  SRR4302535.3813279/94‑1 (MQ=60)
                               CAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACT                                                              <  SRR4302535.232016/94‑1 (MQ=60)
                               CAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACT                                                              >  SRR4302535.138749/1‑94 (MQ=60)
                                AGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCTGGCTGACGGCGCGGTGATCGTCACGCCGGAATATTACTACTC                                                             >  SRR4302535.1929170/1‑94 (MQ=60)
                                 GGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCCGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCG                                                            <  SRR4302535.3323777/94‑1 (MQ=60)
                                 GGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCG                                                            <  SRR4302535.2379717/94‑1 (MQ=60)
                                 GGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCG                                                            <  SRR4302535.1452137/94‑1 (MQ=60)
                                 GGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCG                                                            <  SRR4302535.1891264/94‑1 (MQ=60)
                                 GGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCG                                                            <  SRR4302535.1335394/94‑1 (MQ=60)
                                  GAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGG                                                           <  SRR4302535.2697980/94‑1 (MQ=60)
                                   AAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGT                                                          <  SRR4302535.684671/94‑1 (MQ=60)
                                   AAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGT                                                          <  SRR4302535.1112765/94‑1 (MQ=60)
                                    AGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTA                                                         >  SRR4302535.1111938/1‑94 (MQ=60)
                                     GAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTAC                                                        <  SRR4302535.2930654/94‑1 (MQ=60)
                                       AGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCG                                                      <  SRR4302535.13279/94‑1 (MQ=60)
                                       AGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCG                                                      >  SRR4302535.307750/1‑94 (MQ=60)
                                       AGGTTTTCAAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCG                                                      >  SRR4302535.4004179/1‑94 (MQ=60)
                                        GGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGG                                                     >  SRR4302535.2993316/1‑94 (MQ=60)
                                        GGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGG                                                     >  SRR4302535.1513557/1‑94 (MQ=60)
                                         GTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGG                                                    >  SRR4302535.4707208/1‑94 (MQ=60)
                                               CAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCT                                              >  SRR4302535.1351022/1‑94 (MQ=60)
                                               CAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCT                                              >  SRR4302535.1646130/1‑94 (MQ=60)
                                                AGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTG                                             >  SRR4302535.3207786/1‑94 (MQ=60)
                                                  CAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAA                                           <  SRR4302535.3549497/94‑1 (MQ=60)
                                                  CAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAA                                           >  SRR4302535.2492920/1‑94 (MQ=60)
                                                   AACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATTGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAA                                          <  SRR4302535.4342441/94‑1 (MQ=60)
                                                   AACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAA                                          >  SRR4302535.1441258/1‑94 (MQ=60)
                                                   AACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAA                                          >  SRR4302535.1531877/1‑94 (MQ=60)
                                                   AAAGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAA                                          >  SRR4302535.1740294/1‑94 (MQ=60)
                                                    ACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGAGGTGATCGGCACGCCGGCATATAACTACTCGGTACCGGGTGGCCTGAAAA                                         >  SRR4302535.3094765/1‑94 (MQ=60)
                                                     CGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAA                                        >  SRR4302535.140192/1‑94 (MQ=60)
                                                       GTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATG                                      >  SRR4302535.1429643/1‑94 (MQ=60)
                                                        TTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGC                                     <  SRR4302535.546421/94‑1 (MQ=60)
                                                         TGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGTTCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCC                                    >  SRR4302535.2527185/1‑94 (MQ=60)
                                                         TGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCC                                    >  SRR4302535.1705940/1‑94 (MQ=60)
                                                         TGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCC                                    >  SRR4302535.1760884/1‑94 (MQ=60)
                                                         TGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCC                                    >  SRR4302535.3636762/1‑94 (MQ=60)
                                                          GAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCA                                   >  SRR4302535.1762802/1‑94 (MQ=60)
                                                           AAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCAT                                  <  SRR4302535.4474680/94‑1 (MQ=60)
                                                            AGCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATC                                 >  SRR4302535.767076/1‑94 (MQ=60)
                                                             GCTCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCG                                >  SRR4302535.4278705/1‑94 (MQ=60)
                                                               TCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGAC                              <  SRR4302535.1497078/94‑1 (MQ=60)
                                                               TCTGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGAC                              <  SRR4302535.2175143/94‑1 (MQ=60)
                                                                 TGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTG                            <  SRR4302535.4008490/94‑1 (MQ=60)
                                                                 TGGCGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTG                            >  SRR4302535.4656252/1‑94 (MQ=60)
                                                                    CGGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCT                         <  SRR4302535.434099/94‑1 (MQ=60)
                                                                     GGAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTT                        >  SRR4302535.2362769/1‑94 (MQ=60)
                                                                      GAACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTCGCTTT                       >  SRR4302535.337152/1‑94 (MQ=60)
                                                                       AACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTC                      >  SRR4302535.689120/1‑94 (MQ=60)
                                                                        ACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCC                     <  SRR4302535.1628592/94‑1 (MQ=60)
                                                                        ACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCC                     <  SRR4302535.2156304/94‑1 (MQ=60)
                                                                        ACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCC                     >  SRR4302535.620267/1‑94 (MQ=60)
                                                                        ACAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCC                     <  SRR4302535.2477358/94‑1 (MQ=60)
                                                                         CAGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCC                    <  SRR4302535.3399104/94‑1 (MQ=60)
                                                                          AGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCG                   >  SRR4302535.4206491/1‑94 (MQ=60)
                                                                          AGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCG                   >  SRR4302535.3716197/1‑94 (MQ=60)
                                                                          AGATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCG                   <  SRR4302535.4558404/94‑1 (MQ=60)
                                                                           GATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGC                  >  SRR4302535.2348498/1‑94 (MQ=60)
                                                                            ATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCC                 >  SRR4302535.3346562/1‑94 (MQ=60)
                                                                            ATCCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCC                 >  SRR4302535.4184079/1‑94 (MQ=60)
                                                                             TCCGTCAGGCTGACGGCGGGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCT                <  SRR4302535.1548603/94‑1 (MQ=60)
                                                                              CCGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTG               <  SRR4302535.3599755/94‑1 (MQ=60)
                                                                               CGTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGC              >  SRR4302535.2896213/1‑94 (MQ=60)
                                                                                GTCAGGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCC             >  SRR4302535.2353604/1‑94 (MQ=60)
                                                                                    GGCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTCCCGGAT         >  SRR4302535.3002371/1‑94 (MQ=60)
                                                                                     GCTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATC        <  SRR4302535.2076202/94‑1 (MQ=60)
                                                                                      CTGACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCA       >  SRR4302535.1047579/1‑94 (MQ=60)
                                                                                        GACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAAC     <  SRR4302535.775741/94‑1 (MQ=60)
                                                                                        GACGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAAC     >  SRR4302535.2155755/1‑94 (MQ=60)
                                                                                          CGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCG   <  SRR4302535.441930/94‑1 (MQ=60)
                                                                                          CGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCG   >  SRR4302535.2714779/1‑94 (MQ=60)
                                                                                          CGGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCG   <  SRR4302535.2004963/94‑1 (MQ=60)
                                                                                           GGCGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGC  >  SRR4302535.3283531/1‑94 (MQ=60)
                                                                                             |                                                                                           
CGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGC  >  NC_000913/3892412‑3892596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑