Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,893,993 A→G A82A (GCT→GCC)  yieG ← putative membrane/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,893,9930AG99.8% 1274.6 / ‑5.5 398A82A (GCT→GCC) yieGputative membrane/transport protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (185/212);  total (186/212)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

Only 100 of 409 total aligned reads displayed.

GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAG  >  NC_000913/3893903‑3894082
                                                                                          |                                                                                         
gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGc                                                                                         >  2:6742783/1‑93 (MQ=255)
gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGc                                                                                         >  2:5235113/1‑93 (MQ=255)
 aCCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCa                                                                                        <  2:7524113/93‑1 (MQ=255)
  ccTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCaa                                                                                       >  2:1029789/1‑93 (MQ=255)
    tGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGGAAAc                                                                                     >  2:5762389/1‑93 (MQ=255)
    tGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCaagc                                                                                     <  1:1154829/93‑3 (MQ=255)
    tGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAAc                                                                                     >  1:5355309/1‑93 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                    <  2:3821960/93‑1 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                    <  1:6343925/93‑1 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                    <  1:7317343/93‑1 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                    <  2:4502917/93‑1 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                    <  1:1269087/93‑1 (MQ=255)
         cGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTcc                                                                                <  2:2828781/93‑1 (MQ=255)
           gCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCca                                                                              >  2:250194/1‑93 (MQ=255)
              aGCCCATCGCCTGTACAACGACAAAAGCGAAGAAAGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatca                                                                           >  1:3820218/1‑93 (MQ=255)
               gCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatcat                                                                          >  2:421633/1‑93 (MQ=255)
                 ccATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataa                                                                        >  1:7269565/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                       >  2:848036/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                       >  2:2216639/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                       >  2:5965813/1‑93 (MQ=255)
                   aTCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataata                                                                      <  2:3956735/93‑1 (MQ=255)
                    tCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAc                                                                     >  1:610691/1‑93 (MQ=255)
                     cGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACt                                                                    >  2:5834472/1‑93 (MQ=255)
                      gCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTg                                                                   >  1:7935325/1‑93 (MQ=255)
                       ccTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGc                                                                  <  1:649977/93‑1 (MQ=255)
                        cTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGcc                                                                 >  2:5132641/1‑93 (MQ=255)
                         tGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCg                                                                >  1:976686/1‑93 (MQ=255)
                          gTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGa                                                               >  2:3572949/1‑93 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGaa                                                              >  2:7200591/1‑93 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGaa                                                              <  1:3583769/93‑1 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                             >  1:5216265/1‑93 (MQ=255)
                             cAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATg                                                            >  1:5960017/1‑93 (MQ=255)
                              caCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGc                                                           <  1:2564435/92‑1 (MQ=255)
                              aaCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGc                                                           <  1:4153001/93‑1 (MQ=255)
                              aaCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGc                                                           >  1:4309784/1‑93 (MQ=255)
                                 gACAAAAGCGAAGAACGCATTCAAGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGc                                                        <  2:5394805/93‑1 (MQ=255)
                                  aCAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCg                                                       >  2:5665727/1‑93 (MQ=255)
                                   cAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGa                                                      >  2:20202/1‑93 (MQ=255)
                                    aaaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAt                                                     <  2:730804/93‑1 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                    >  1:6372450/1‑93 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                    <  2:7390844/93‑1 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                    <  2:3332035/93‑1 (MQ=255)
                                       aGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAg                                                  <  1:2920954/93‑1 (MQ=255)
                                        gCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGa                                                 >  2:6934050/1‑93 (MQ=255)
                                        gCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGa                                                 <  1:13569/93‑1 (MQ=255)
                                           aagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAg                                              <  2:5011041/93‑1 (MQ=255)
                                           aagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAg                                              <  1:7169343/93‑1 (MQ=255)
                                            agaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAgg                                             <  1:1215531/93‑1 (MQ=255)
                                              aaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTa                                           <  1:908415/93‑1 (MQ=255)
                                              aaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTa                                           >  1:2360903/1‑93 (MQ=255)
                                              aaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTa                                           >  2:6592099/1‑93 (MQ=255)
                                              aaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTa                                           <  2:1665100/93‑1 (MQ=255)
                                               aCGCATTCAGGCCAATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATCCTGCCGAATGCAGCGATCAGACAGGTAg                                          <  2:7150700/93‑1 (MQ=255)
                                                cGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAgg                                         >  2:6819959/1‑92 (MQ=255)
                                                cGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGt                                         <  1:6293893/93‑1 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       >  1:2097192/1‑93 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       >  2:4873011/1‑93 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                       >  1:2836613/1‑93 (MQ=255)
                                                   aTTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAc                                      <  1:4434178/93‑1 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                     >  2:2848523/1‑93 (MQ=255)
                                                     tCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGa                                    <  2:4632550/93‑1 (MQ=255)
                                                      cAGGGCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGaa                                   <  2:5511080/93‑1 (MQ=255)
                                                       aGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAg                                  >  1:3360130/1‑93 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 <  2:2288228/93‑1 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 >  1:7793938/1‑93 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 >  2:7713483/1‑93 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 <  1:4643982/93‑1 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 >  1:6688013/1‑93 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 >  1:3249366/1‑93 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 <  2:1501259/93‑1 (MQ=255)
                                                        ggCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGa                                 <  2:1894205/93‑1 (MQ=255)
                                                          cccATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACg                               <  1:2209933/93‑1 (MQ=255)
                                                           ccATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACgt                              >  2:3631550/1‑92 (MQ=255)
                                                           ccATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACgt                              <  2:7858543/93‑2 (MQ=255)
                                                           ccATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACgt                              <  1:3202514/93‑2 (MQ=255)
                                                            cATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACgtc                             <  1:1821420/93‑3 (MQ=255)
                                                              tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                           >  1:1438742/1‑93 (MQ=255)
                                                                gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                         <  1:3613102/93‑1 (MQ=255)
                                                                gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                         <  1:3086496/93‑1 (MQ=255)
                                                                 cGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTgg                        <  1:1536750/93‑1 (MQ=255)
                                                                     tGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATc                    <  2:1333105/93‑1 (MQ=255)
                                                                      gCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATcc                   <  2:468857/93‑1 (MQ=255)
                                                                       ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                  >  1:5636701/1‑93 (MQ=255)
                                                                       ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                  <  1:5487632/93‑1 (MQ=255)
                                                                        cAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAt                 <  1:4805579/93‑1 (MQ=255)
                                                                         aGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATg                >  2:3133429/1‑93 (MQ=255)
                                                                          gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGc               >  2:3453612/1‑93 (MQ=255)
                                                                           tGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGcc              <  1:4938204/93‑1 (MQ=255)
                                                                              aaCTGGCAGGTTGGCAAACCGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc           <  1:7695420/93‑1 (MQ=255)
                                                                              aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc           >  1:2442568/1‑93 (MQ=255)
                                                                                cTGGCCGGTTGGCAAGAAGTCCCATCATAATCCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCaa         <  1:6624630/93‑1 (MQ=255)
                                                                                cTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCaa         <  2:6091285/93‑1 (MQ=255)
                                                                                cTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCaa         >  2:6416771/1‑93 (MQ=255)
                                                                                cTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCaa         <  1:7458649/93‑1 (MQ=255)
                                                                                 tGGCAGGTAGGCAAACAGTCCCATCATAAAACTGCCGAATGCAGAGATCAGACAGGAAGAGACGAAGACGTCGCTGGTATCCATGACAGCAAc        <  1:6994578/93‑1 (MQ=255)
                                                                                  gacAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGAAACg       <  2:4095081/91‑1 (MQ=255)
                                                                                   gCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGc      <  2:1254303/93‑1 (MQ=255)
                                                                                    cAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGcc     >  2:6241089/1‑93 (MQ=255)
                                                                                      ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa   >  1:6869893/1‑93 (MQ=255)
                                                                                       gTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAg  >  2:1422554/1‑93 (MQ=255)
                                                                                          |                                                                                         
GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAG  >  NC_000913/3893903‑3894082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 217 total aligned reads displayed.
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATT  >  NC_000913/3893900‑3894086
                                                                                             |                                                                                             
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               >  SRR4302543.7314536/1‑94 (MQ=60)
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               <  SRR4302543.6239039/94‑1 (MQ=60)
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               <  SRR4302543.5855319/94‑1 (MQ=60)
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               >  SRR4302543.6971236/1‑94 (MQ=60)
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               <  SRR4302543.4079538/94‑1 (MQ=60)
CACGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               <  SRR4302543.7494861/94‑1 (MQ=60)
 CCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGG                                                                                              >  SRR4302543.1273676/1‑94 (MQ=60)
 CCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGG                                                                                              >  SRR4302543.7451315/1‑94 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACTACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                             <  SRR4302543.1534791/94‑1 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                             >  SRR4302543.5449422/1‑94 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                             >  SRR4302543.6598700/1‑94 (MQ=60)
   GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCA                                                                                            <  SRR4302543.2886857/94‑1 (MQ=60)
    ACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAA                                                                                           <  SRR4302543.3618141/94‑1 (MQ=60)
      CTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAAC                                                                                         >  SRR4302543.6241408/1‑94 (MQ=60)
      CTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAAC                                                                                         >  SRR4302543.5403779/1‑94 (MQ=60)
      CTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAAC                                                                                         <  SRR4302543.3332733/94‑1 (MQ=60)
       TGCCGCTGCAAGCCCATCGGCCGGACGACGACAAAAGCGAAGAACGCCTGCAGGCCCAGAGCGGGTTCCAGCGCAACTGGCAGGTTGGCAAACA                                                                                        <  SRR4302543.2285529/94‑1 (MQ=28)
       TGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAATTGGCAGGTTGGCACACA                                                                                        >  SRR4302543.7613110/1‑94 (MQ=60)
       TGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAGCA                                                                                        <  SRR4302543.1165336/94‑1 (MQ=60)
       TGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACA                                                                                        >  SRR4302543.7613111/1‑94 (MQ=60)
       TGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACA                                                                                        >  SRR4302543.2013149/1‑94 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302543.1280434/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302543.455395/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302543.7387842/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302543.2917040/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302543.6403581/94‑1 (MQ=60)
        GCCACGGCAAGCCCAGCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302543.440590/94‑1 (MQ=60)
          CACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTC                                                                                     >  SRR4302543.1218487/1‑94 (MQ=60)
          CACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTC                                                                                     <  SRR4302543.2495212/94‑1 (MQ=60)
            CGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCC                                                                                   <  SRR4302543.7860440/94‑1 (MQ=60)
             GGCAAGCCCACCGCCTGTACAACGACAGAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCA                                                                                  <  SRR4302543.811391/94‑1 (MQ=60)
              GCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCAT                                                                                 >  SRR4302543.6365199/1‑94 (MQ=60)
              GCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCAT                                                                                 <  SRR4302543.477367/94‑1 (MQ=60)
               CAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATC                                                                                >  SRR4302543.2804638/1‑94 (MQ=60)
               CAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATC                                                                                <  SRR4302543.2567145/94‑1 (MQ=60)
                AAGCCCATCGCCTGTACAACGACAAAAGCGAAGAAAGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCA                                                                               >  SRR4302543.3853907/1‑94 (MQ=60)
                 AGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCAT                                                                              >  SRR4302543.6906585/1‑94 (MQ=60)
                 AGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCAT                                                                              >  SRR4302543.1720924/1‑94 (MQ=60)
                   CCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAA                                                                            >  SRR4302543.1004829/1‑94 (MQ=60)
                   CCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAA                                                                            >  SRR4302543.7339795/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           <  SRR4302543.4908529/94‑1 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           <  SRR4302543.7010296/94‑1 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           <  SRR4302543.7010295/94‑1 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAC                                                                         >  SRR4302543.616268/1‑94 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAC                                                                         >  SRR4302543.7267817/1‑94 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGGGCAACTGTCAGGATGGCAAACAGTCCCATCATAATAC                                                                         >  SRR4302543.3743693/1‑94 (MQ=60)
                       TCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACT                                                                        <  SRR4302543.1236158/94‑1 (MQ=60)
                        CGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATCCTG                                                                       >  SRR4302543.6424529/1‑94 (MQ=60)
                        CGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTG                                                                       >  SRR4302543.8009811/1‑94 (MQ=60)
                         GCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGC                                                                      >  SRR4302543.3626528/1‑94 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     <  SRR4302543.6434056/94‑1 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     >  SRR4302543.3720319/1‑94 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     <  SRR4302543.914465/94‑1 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     <  SRR4302543.1423629/94‑1 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     <  SRR4302543.655924/94‑1 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAGCAGTCCCATCATAATACTGCCG                                                                    >  SRR4302543.3165791/1‑94 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCG                                                                    >  SRR4302543.3632448/1‑94 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCG                                                                    >  SRR4302543.6210652/1‑94 (MQ=60)
                            TGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGA                                                                   >  SRR4302543.5415384/1‑94 (MQ=60)
                             GTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAA                                                                  >  SRR4302543.7983940/1‑94 (MQ=60)
                             GGACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGCGCCAGTGCAACTGGCAGGGTGGAAAAAAGGCCCATCATAATACTGCCGAA                                                                  >  SRR4302543.2554138/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 >  SRR4302543.5263256/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302543.3615422/94‑1 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302543.4508643/94‑1 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGCCCCATGGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302543.4508644/94‑1 (MQ=60)
                              GACAACGACAAAAGCGAAGANNGNATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302543.2817399/94‑1 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                                >  SRR4302543.6014581/1‑94 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                                <  SRR4302543.3387521/94‑1 (MQ=60)
                                CAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGC                                                               >  SRR4302543.4348929/1‑94 (MQ=60)
                                 CACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                              <  SRR4302543.2587866/94‑1 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                              <  SRR4302543.4190547/94‑1 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                              <  SRR4302543.5889782/94‑1 (MQ=60)
                                  ACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAG                                                             <  SRR4302543.6378749/94‑1 (MQ=60)
                                   CGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGC                                                            <  SRR4302543.680878/94‑1 (MQ=60)
                                    GACAAAATCGAAGAACGCATTCAGGCCCATAGCTGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           <  SRR4302543.5278297/94‑1 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           <  SRR4302543.5278298/94‑1 (MQ=60)
                                     ACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGA                                                          >  SRR4302543.1008325/1‑94 (MQ=60)
                                      CAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAT                                                         <  SRR4302543.733814/94‑1 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        >  SRR4302543.6432392/1‑94 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        >  SRR4302543.7368580/1‑94 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                       <  SRR4302543.6526769/94‑1 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGATGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCG                                                       >  SRR4302543.1187493/1‑94 (MQ=60)
                                         AAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAG                                                      <  SRR4302543.3807460/94‑1 (MQ=60)
                                         AAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAG                                                      >  SRR4302543.6768948/1‑94 (MQ=60)
                                          AGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGA                                                     <  SRR4302543.2947493/94‑1 (MQ=60)
                                           GCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAGCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAC                                                    <  SRR4302543.3061028/94‑1 (MQ=60)
                                           GCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAC                                                    <  SRR4302543.13688/94‑1 (MQ=60)
                                           GCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAC                                                    <  SRR4302543.5033533/94‑1 (MQ=60)
                                             GAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAG                                                  >  SRR4302543.313406/1‑94 (MQ=60)
                                              AAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGG                                                 <  SRR4302543.7238966/94‑1 (MQ=60)
                                              AAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGG                                                 <  SRR4302543.1482742/94‑1 (MQ=60)
                                              AAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGG                                                 >  SRR4302543.7295945/1‑94 (MQ=60)
                                              AAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGG                                                 <  SRR4302543.4722843/94‑1 (MQ=60)
                                               AGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGT                                                <  SRR4302543.1226450/94‑1 (MQ=60)
                                                GAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTA                                               >  SRR4302543.2382312/1‑94 (MQ=60)
                                                 AACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                              <  SRR4302543.916408/94‑1 (MQ=60)
                                                 AACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAG                                              <  SRR4302543.7798153/94‑1 (MQ=60)
                                                  ACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAGCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGT                                             <  SRR4302543.976713/94‑1 (MQ=60)
                                                  ACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGT                                             <  SRR4302543.6070033/94‑1 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            <  SRR4302543.6353140/94‑1 (MQ=60)
                                                   CGCATTCAGGCCAATAGCGGGTGCCAGTGCAACTGGCAAGTTGGCAAACAGACCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            <  SRR4302543.7078133/94‑1 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           <  SRR4302543.6424159/94‑1 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           >  SRR4302543.2115941/1‑94 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           >  SRR4302543.2862553/1‑94 (MQ=60)
                                                     CATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAC                                          >  SRR4302543.2686377/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         <  SRR4302543.3052621/94‑1 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302543.1800042/1‑94 (MQ=60)
                                                        TCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAA                                       <  SRR4302543.5136816/94‑1 (MQ=60)
                                                        TCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAA                                       >  SRR4302543.2390367/1‑94 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCGATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                      >  SRR4302543.1365598/1‑94 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                      <  SRR4302543.140545/94‑1 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                      >  SRR4302543.6378329/1‑94 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                      >  SRR4302543.2045574/1‑94 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                      >  SRR4302543.3390351/1‑94 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                      >  SRR4302543.1365597/1‑94 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     >  SRR4302543.3278744/1‑94 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     >  SRR4302543.2155166/1‑94 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     >  SRR4302543.6751142/1‑94 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     >  SRR4302543.3326491/1‑94 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     >  SRR4302543.97419/1‑94 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                    <  SRR4302543.4686239/94‑1 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                    >  SRR4302543.3948846/1‑94 (MQ=60)
                                                           GGCCCAAAGCGTGTGCCAGTGCAACTGGCAGGGTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                    <  SRR4302543.2398042/94‑1 (MQ=60)
                                                            GCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACG                                   <  SRR4302543.4313890/94‑1 (MQ=60)
                                                            GCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACG                                   <  SRR4302543.7500513/94‑1 (MQ=60)
                                                            GCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACG                                   <  SRR4302543.7350312/94‑1 (MQ=60)
                                                            GCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACG                                   <  SRR4302543.6697666/94‑1 (MQ=60)
                                                             CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                  >  SRR4302543.3421328/1‑94 (MQ=60)
                                                             CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                  <  SRR4302543.2229839/94‑1 (MQ=60)
                                                             CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                  <  SRR4302543.6080897/94‑1 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 >  SRR4302543.5786633/1‑94 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 <  SRR4302543.4846529/94‑1 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 <  SRR4302543.3231395/94‑1 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 >  SRR4302543.2945748/1‑94 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 <  SRR4302543.3962781/94‑1 (MQ=60)
                                                              CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                 <  SRR4302543.1245242/94‑1 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                >  SRR4302543.6743067/1‑94 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                <  SRR4302543.1837761/94‑1 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                >  SRR4302543.5044364/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                               >  SRR4302543.1451741/1‑94 (MQ=60)
                                                                  AGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCGGCGATCAGACAGGTAGTGACGAAGACGTCGCTG                             <  SRR4302543.4254416/94‑1 (MQ=60)
                                                                   GCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGGCGTCGCTGG                            >  SRR4302543.1447141/1‑94 (MQ=60)
                                                                   GCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGG                            <  SRR4302543.978270/94‑1 (MQ=60)
                                                                   GCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGG                            <  SRR4302543.3644960/94‑1 (MQ=60)
                                                                    GGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                           <  SRR4302543.1509253/94‑1 (MQ=60)
                                                                    CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                           <  SRR4302543.2428075/94‑1 (MQ=60)
                                                                    CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                           >  SRR4302543.1779502/1‑94 (MQ=60)
                                                                    CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                           <  SRR4302543.3185403/94‑1 (MQ=60)
                                                                     GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                          >  SRR4302543.4439530/1‑94 (MQ=60)
                                                                     GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGACCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                          >  SRR4302543.4268364/1‑94 (MQ=60)
                                                                      GGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAT                         <  SRR4302543.7638623/94‑1 (MQ=60)
                                                                      GGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAT                         <  SRR4302543.4370584/94‑1 (MQ=60)
                                                                      GGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAT                         >  SRR4302543.59990/1‑94 (MQ=60)
                                                                       GTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATC                        >  SRR4302543.5306153/1‑94 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                       <  SRR4302543.6285828/94‑1 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCac                      >  SRR4302543.3259896/1‑92 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                      >  SRR4302543.1197318/1‑94 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                      >  SRR4302543.5688080/1‑94 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302543.3242061/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302543.4935742/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302543.6355560/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302543.5537388/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAGGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302543.4935748/94‑1 (MQ=60)
                                                                           CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                    <  SRR4302543.4849479/94‑1 (MQ=60)
                                                                            AGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGC                   <  SRR4302543.7669781/94‑1 (MQ=60)
                                                                             GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                  >  SRR4302543.6468923/1‑94 (MQ=60)
                                                                             GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                  <  SRR4302543.2205095/94‑1 (MQ=60)
                                                                             GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                  >  SRR4302543.6973636/1‑94 (MQ=60)
                                                                              TGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCA                 <  SRR4302543.84180/94‑1 (MQ=60)
                                                                                CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               >  SRR4302543.5478840/1‑94 (MQ=60)
                                                                                CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               >  SRR4302543.1532975/1‑94 (MQ=60)
                                                                                CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               >  SRR4302543.2464589/1‑94 (MQ=60)
                                                                                 AACTGGCAGGTTGGCAAACCGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA              <  SRR4302543.7768263/94‑1 (MQ=60)
                                                                                 AACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA              >  SRR4302543.5630105/1‑94 (MQ=60)
                                                                                  ACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAA             >  SRR4302543.7219444/1‑94 (MQ=60)
                                                                                   CTGGCCGGTTGGCAAGAAGTCCCATCATAATCCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            <  SRR4302543.6687107/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            <  SRR4302543.2374563/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            <  SRR4302543.2146453/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            >  SRR4302543.6577402/1‑94 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            <  SRR4302543.1228003/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            <  SRR4302543.441748/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAAC            <  SRR4302543.7530097/94‑1 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           <  SRR4302543.3238735/94‑1 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           <  SRR4302543.3458530/94‑1 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           >  SRR4302543.7778170/1‑94 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           <  SRR4302543.5671174/94‑1 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           >  SRR4302543.7493984/1‑94 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           >  SRR4302543.793627/1‑94 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           <  SRR4302543.2964825/94‑1 (MQ=60)
                                                                                    TGGCAGGTAGGCAAACAGTCCCATCATAAAACTGCCGAATGCAGAGATCAGACAGGAAGAGACGAAGACGTCGCTGGTATCCATGACAGCAACG           <  SRR4302543.7060511/94‑1 (MQ=60)
                                                                                       CAGGTTGGCAAGCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCA        <  SRR4302543.6058128/94‑1 (MQ=60)
                                                                                       CAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCA        <  SRR4302543.7275382/94‑1 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA       >  SRR4302543.794592/1‑94 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA       <  SRR4302543.2023179/94‑1 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA       >  SRR4302543.6646074/1‑94 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA       >  SRR4302543.6934596/1‑94 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      <  SRR4302543.231019/94‑1 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT   >  SRR4302543.1472929/1‑94 (MQ=60)
                                                                                             GGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT  <  SRR4302543.3651587/94‑1 (MQ=60)
                                                                                             GGCAAACAGCCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT  <  SRR4302543.6005084/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATT  >  NC_000913/3893900‑3894086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑