Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,894,054 G→T A62D (GCC→GAC)  yieG ← putative membrane/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,894,0540GT99.1% 1504.4 / ‑1.2 424A62D (GCC→GAC) yieGputative membrane/transport protein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (199/221);  total (201/223)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 438 total aligned reads displayed.

ATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC  >  NC_000913/3893964‑3894143
                                                                                          |                                                                                         
aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                                                                                         >  2:2513517/1‑93 (MQ=255)
 tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                                                                                        >  2:4402421/1‑93 (MQ=255)
  aGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCGGCGATCAGACAGGTAGTGACGAAGACGTCGCt                                                                                       <  1:4216189/93‑1 (MQ=255)
   gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                                                                                      <  2:1995921/93‑1 (MQ=255)
   gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                                                                                      <  1:969742/93‑1 (MQ=255)
      ggTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTa                                                                                   >  2:5188815/1‑93 (MQ=255)
        tGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATc                                                                                 >  1:5258731/1‑93 (MQ=255)
        tGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATc                                                                                 >  1:7027469/1‑93 (MQ=255)
         gCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATcc                                                                                <  2:468857/93‑1 (MQ=255)
          ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                                                                               <  1:3213046/93‑1 (MQ=255)
            aGTGCAACTGGCAGGTGGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATg                                                                             <  2:3616786/93‑1 (MQ=255)
             gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGc                                                                            >  2:3453612/1‑93 (MQ=255)
             gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGc                                                                            >  2:5117022/1‑93 (MQ=255)
              tGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCATACAGGTAGTGACGAAGACGTCGCTGGTATCCATGcc                                                                           >  2:5814327/1‑93 (MQ=255)
              tGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGcc                                                                           <  1:83409/93‑1 (MQ=255)
               gCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCa                                                                          >  2:899756/1‑93 (MQ=255)
               gCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCa                                                                          <  2:7157891/93‑1 (MQ=255)
                 aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc                                                                        >  1:5548319/1‑93 (MQ=255)
                   cTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCaa                                                                      <  1:437647/93‑1 (MQ=255)
                   cTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCaa                                                                      >  1:7149948/1‑93 (MQ=255)
                     ggCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACg                                                                    >  1:786704/1‑93 (MQ=255)
                     gacAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGAAACg                                                                    <  2:4095081/91‑1 (MQ=255)
                       cAGGTTGGCAAGCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGcc                                                                  <  1:6002931/93‑1 (MQ=255)
                       cAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGcc                                                                  <  2:3168311/93‑1 (MQ=255)
                        aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa                                                                 >  2:6120661/1‑93 (MQ=255)
                           ttggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGa                                                              >  2:5255634/4‑93 (MQ=255)
                           ttggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGa                                                              >  2:5380048/4‑93 (MQ=255)
                            tggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGaa                                                             >  2:815423/3‑93 (MQ=255)
                             ggCAAACAGTCCCATCATAATACTGCCGTATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGCATCCATGCCAGCAACGCCAAGAAt                                                            >  2:1206612/2‑93 (MQ=255)
                             ggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAt                                                            >  1:1459764/2‑93 (MQ=255)
                             ggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAt                                                            <  1:3619691/92‑1 (MQ=255)
                                aaaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTg                                                         <  1:1712643/93‑1 (MQ=255)
                                aaaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTg                                                         >  2:1706272/1‑93 (MQ=255)
                                  aCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCg                                                       >  1:7710122/1‑93 (MQ=255)
                                  aCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCg                                                       >  1:3783493/1‑93 (MQ=255)
                                    aGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCggg                                                     <  1:661933/93‑1 (MQ=255)
                                    aGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCggg                                                     >  1:152555/1‑93 (MQ=255)
                                    aGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCggg                                                     <  2:7620694/93‑1 (MQ=255)
                                      tCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGtt                                                   >  1:7405724/1‑93 (MQ=255)
                                       cccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTa                                                  <  1:2241535/93‑1 (MQ=255)
                                       cccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTa                                                  >  1:6264531/1‑93 (MQ=255)
                                        ccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTaa                                                 >  2:7883521/1‑93 (MQ=255)
                                           tcatAATACTGCCGAATTCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaa                                              <  1:3399756/93‑1 (MQ=255)
                                           tcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaa                                              >  2:4747922/1‑93 (MQ=255)
                                           tcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaa                                              <  2:2342624/93‑1 (MQ=255)
                                           tcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaa                                              <  2:6766593/93‑1 (MQ=255)
                                             ataataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaaa                                            >  1:5058758/1‑93 (MQ=255)
                                              taataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGTCTGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGCGTTAACaaaaa                                           >  1:2093662/1‑93 (MQ=255)
                                              taataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaaaa                                           <  1:1809096/93‑1 (MQ=255)
                                              taataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaaaa                                           >  2:6962641/1‑93 (MQ=255)
                                               aataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAAc                                          <  1:5626873/93‑1 (MQ=255)
                                                  aCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGAt                                       <  1:6951437/93‑1 (MQ=255)
                                                   cTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATg                                      >  2:3319430/1‑93 (MQ=255)
                                                   cTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATg                                      >  1:3020159/1‑93 (MQ=255)
                                                    tGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATga                                     >  1:2675187/1‑92 (MQ=255)
                                                    tGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGt                                     >  2:2797087/1‑93 (MQ=255)
                                                    tGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGt                                     <  1:105899/93‑1 (MQ=255)
                                                       cGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaaa                                  <  2:206229/93‑1 (MQ=255)
                                                       cGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaaa                                  <  1:758437/93‑1 (MQ=255)
                                                        gAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAc                                 <  2:1483051/93‑1 (MQ=255)
                                                        gAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAc                                 <  2:6059668/93‑1 (MQ=255)
                                                        gAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAc                                 >  1:13585/1‑93 (MQ=255)
                                                        gAACGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAc                                 <  1:4704951/93‑1 (MQ=255)
                                                         aaTGCAGCGGTCAGACATGTAGTGACGAAGACCGCGCTGGTATCCATGCCAGCAACGCCAAGGCTTTTCGGGTTAACCAACACGATGTAAAcc                                >  2:7401878/1‑93 (MQ=255)
                                                         aaTGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAcc                                <  1:4912117/93‑1 (MQ=255)
                                                         aaTGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAcc                                >  2:5621946/1‑93 (MQ=255)
                                                          aTGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCa                               >  2:6262892/1‑93 (MQ=255)
                                                           tGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAt                              >  2:4861217/1‑93 (MQ=255)
                                                           tGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAt                              >  1:6428123/1‑93 (MQ=255)
                                                           tGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAt                              <  2:3778745/93‑1 (MQ=255)
                                                           tGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAt                              <  1:594158/93‑1 (MQ=255)
                                                           tGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAt                              >  2:7675532/1‑93 (MQ=255)
                                                             cAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCg                            <  2:7522158/93‑1 (MQ=255)
                                                               gCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTc                          >  2:1860768/1‑93 (MQ=255)
                                                                cGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCa                         >  2:1960075/1‑93 (MQ=255)
                                                                 gATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAg                        <  1:6880282/93‑1 (MQ=255)
                                                                 gATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAg                        >  1:2467642/1‑93 (MQ=255)
                                                                 gATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAg                        >  1:5764158/1‑93 (MQ=255)
                                                                  aTCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAgg                       >  2:1319360/1‑93 (MQ=255)
                                                                   tCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGa                      >  2:6441809/1‑93 (MQ=255)
                                                                   tCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGa                      >  2:3059026/1‑93 (MQ=255)
                                                                    cAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGaa                     >  2:297377/1‑93 (MQ=255)
                                                                    cAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGaa                     <  1:5474191/93‑1 (MQ=255)
                                                                     aGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAg                    <  2:6364675/93‑1 (MQ=255)
                                                                       aCAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggt                  <  1:4068039/93‑1 (MQ=255)
                                                                       aCAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGACGTAAACCATCGTCAGGAAggg                  >  2:1403256/1‑92 (MQ=255)
                                                                         aGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgg                >  1:7806200/1‑93 (MQ=255)
                                                                         aGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgg                >  1:6078086/1‑93 (MQ=255)
                                                                         aGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgg                <  1:1217799/93‑1 (MQ=255)
                                                                         aGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgg                <  1:6742784/93‑1 (MQ=255)
                                                                         aGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgg                >  1:7238589/1‑93 (MQ=255)
                                                                          ggTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtggt               >  2:2272802/1‑93 (MQ=255)
                                                                              gTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTaaaa           <  2:6584061/93‑1 (MQ=255)
                                                                              gTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTaaaa           >  2:7323094/1‑93 (MQ=255)
                                                                                gACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAcc         <  1:4814285/93‑1 (MQ=255)
                                                                                 aCGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCg        >  1:7685304/1‑93 (MQ=255)
                                                                                    aaGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCg     >  1:3470571/1‑93 (MQ=255)
                                                                                     aGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTACAACCGGCGa    >  1:2354942/1‑93 (MQ=255)
                                                                                     aGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGa    <  1:7485966/93‑1 (MQ=255)
                                                                                       aCGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATc  >  1:2850254/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC  >  NC_000913/3893964‑3894143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 249 total aligned reads displayed.
CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACTT  >  NC_000913/3893961‑3894147
                                                                                             |                                                                                             
CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                                                                               <  SRR4302543.2229839/94‑1 (MQ=60)
CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                                                                               <  SRR4302543.6080897/94‑1 (MQ=60)
 CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                                                                              <  SRR4302543.4846529/94‑1 (MQ=60)
 CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                                                                              >  SRR4302543.2945748/1‑94 (MQ=60)
 CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                                                                              <  SRR4302543.3231395/94‑1 (MQ=60)
 CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                                                                              <  SRR4302543.1245242/94‑1 (MQ=60)
 CCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTC                                                                                              <  SRR4302543.3962781/94‑1 (MQ=60)
  CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                                                                             >  SRR4302543.6743067/1‑94 (MQ=60)
  CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                                                                             <  SRR4302543.1837761/94‑1 (MQ=60)
     AGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCGGCGATCAGACAGGTAGTGACGAAGACGTCGCTG                                                                                          <  SRR4302543.4254416/94‑1 (MQ=60)
      GCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGGCGTCGCTGG                                                                                         >  SRR4302543.1447141/1‑94 (MQ=60)
       GGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                                                                                        <  SRR4302543.1509253/94‑1 (MQ=60)
       CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                                                                                        <  SRR4302543.1344185/94‑1 (MQ=60)
       CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                                                                                        <  SRR4302543.2428075/94‑1 (MQ=60)
       CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                                                                                        <  SRR4302543.3185403/94‑1 (MQ=60)
       CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                                                                                        <  SRR4302543.1550508/94‑1 (MQ=60)
       CGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGT                                                                                        >  SRR4302543.1779502/1‑94 (MQ=60)
        GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                                                                                       >  SRR4302543.4439530/1‑94 (MQ=60)
        GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGACCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                                                                                       >  SRR4302543.4268364/1‑94 (MQ=60)
         GGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAT                                                                                      >  SRR4302543.59990/1‑94 (MQ=60)
         GGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAT                                                                                      <  SRR4302543.7638623/94‑1 (MQ=60)
          GTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATC                                                                                     >  SRR4302543.5306153/1‑94 (MQ=60)
          GTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATC                                                                                     >  SRR4302543.7094935/1‑94 (MQ=60)
           TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                                                                                    <  SRR4302543.6285828/94‑1 (MQ=60)
            GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCac                                                                                   >  SRR4302543.3259896/1‑92 (MQ=60)
            GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                                                                                   >  SRR4302543.5688080/1‑94 (MQ=60)
            GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                                                                                   >  SRR4302543.1197318/1‑94 (MQ=60)
             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                  <  SRR4302543.5537388/94‑1 (MQ=60)
             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                  <  SRR4302543.4935742/94‑1 (MQ=60)
             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                  <  SRR4302543.3242061/94‑1 (MQ=60)
             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                  <  SRR4302543.6355560/94‑1 (MQ=60)
             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAGGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                  <  SRR4302543.4935748/94‑1 (MQ=60)
              CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                                                                                 <  SRR4302543.4849479/94‑1 (MQ=60)
              CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                                                                                 <  SRR4302543.6707718/94‑1 (MQ=60)
                GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                                                                               >  SRR4302543.6468923/1‑94 (MQ=60)
                GTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCC                                                                               <  SRR4302543.2205095/94‑1 (MQ=60)
                 TGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCA                                                                              <  SRR4302543.4983172/94‑1 (MQ=60)
                 TGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCA                                                                              <  SRR4302543.84180/94‑1 (MQ=60)
                   CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC                                                                            >  SRR4302543.5478840/1‑94 (MQ=60)
                   CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC                                                                            >  SRR4302543.1532975/1‑94 (MQ=60)
                   CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC                                                                            >  SRR4302543.2464589/1‑94 (MQ=60)
                   CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC                                                                            >  SRR4302543.5598777/1‑94 (MQ=60)
                    AACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA                                                                           >  SRR4302543.5630105/1‑94 (MQ=60)
                     ACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAA                                                                          >  SRR4302543.7219444/1‑94 (MQ=60)
                      cgcgcaggttgtaaaaccGTCCCATCATAATACGGCCGAATGAAGCGATAAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                         <  SRR4302543.441747/76‑1 (MQ=60)
                      CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                         >  SRR4302543.6577402/1‑94 (MQ=60)
                      CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                         <  SRR4302543.1228003/94‑1 (MQ=60)
                      CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                         <  SRR4302543.2146453/94‑1 (MQ=60)
                      CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                         <  SRR4302543.441748/94‑1 (MQ=60)
                      CTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAAC                                                                         <  SRR4302543.7530097/94‑1 (MQ=60)
                       TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        >  SRR4302543.7778170/1‑94 (MQ=60)
                       TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        >  SRR4302543.793627/1‑94 (MQ=60)
                       TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        <  SRR4302543.3458530/94‑1 (MQ=60)
                       TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        >  SRR4302543.7493984/1‑94 (MQ=60)
                       TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        <  SRR4302543.2964825/94‑1 (MQ=60)
                       TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                        <  SRR4302543.5671174/94‑1 (MQ=60)
                          CAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCA                                                                     <  SRR4302543.7275382/94‑1 (MQ=60)
                           agtgtgGCAAACAGTCCCATCATAATACGGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA                                                                    <  SRR4302543.2023178/88‑1 (MQ=60)
                           AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA                                                                    >  SRR4302543.6646074/1‑94 (MQ=60)
                           AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA                                                                    >  SRR4302543.6934596/1‑94 (MQ=60)
                           AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA                                                                    >  SRR4302543.794592/1‑94 (MQ=60)
                           AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA                                                                    <  SRR4302543.2023179/94‑1 (MQ=60)
                            GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG                                                                   <  SRR4302543.231019/94‑1 (MQ=60)
                               TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT                                                                >  SRR4302543.1472929/1‑94 (MQ=60)
                                GGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT                                                               <  SRR4302543.3651587/94‑1 (MQ=60)
                                GGCAAACAGCCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT                                                               <  SRR4302543.6005084/94‑1 (MQ=60)
                                 GCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTT                                                              >  SRR4302543.5127920/1‑94 (MQ=60)
                                 GCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTT                                                              >  SRR4302543.5595065/1‑94 (MQ=60)
                                  CAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTG                                                             >  SRR4302543.895299/1‑94 (MQ=60)
                                  CAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTG                                                             <  SRR4302543.905342/94‑1 (MQ=60)
                                   AAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGC                                                            >  SRR4302543.1077560/1‑94 (MQ=60)
                                   AAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGC                                                            >  SRR4302543.902187/1‑94 (MQ=60)
                                   AAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGC                                                            >  SRR4302543.3620366/1‑94 (MQ=60)
                                    AACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                           >  SRR4302543.3444913/1‑94 (MQ=60)
                                    AACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                           >  SRR4302543.7783056/1‑94 (MQ=60)
                                    AACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                           >  SRR4302543.704044/1‑94 (MQ=60)
                                      CAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGG                                                         >  SRR4302543.7306299/1‑94 (MQ=60)
                                       AGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGT                                                        <  SRR4302543.5401507/94‑1 (MQ=60)
                                       AGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGT                                                        <  SRR4302543.668015/94‑1 (MQ=60)
                                        GTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTT                                                       >  SRR4302543.712405/1‑94 (MQ=60)
                                        GTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTT                                                       <  SRR4302543.259549/94‑1 (MQ=60)
                                         TCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTA                                                      >  SRR4302543.6073709/1‑94 (MQ=60)
                                         TCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTA                                                      >  SRR4302543.6323597/1‑94 (MQ=60)
                                          CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                     <  SRR4302543.1728505/94‑1 (MQ=60)
                                          CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                     <  SRR4302543.2261857/94‑1 (MQ=60)
                                          CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                     <  SRR4302543.2261856/94‑1 (MQ=60)
                                          CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                     <  SRR4302543.7300689/94‑1 (MQ=60)
                                            CATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACA                                                   >  SRR4302543.1499550/1‑94 (MQ=60)
                                            CATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACA                                                   >  SRR4302543.2997788/1‑94 (MQ=60)
                                            CATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACA                                                   >  SRR4302543.1557240/1‑94 (MQ=60)
                                             ATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAA                                                  >  SRR4302543.6170798/1‑94 (MQ=60)
                                             ATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAA                                                  >  SRR4302543.3724673/1‑94 (MQ=60)
                                             ATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAA                                                  >  SRR4302543.4750757/1‑94 (MQ=60)
                                             ATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAA                                                  >  SRR4302543.3950000/1‑94 (MQ=60)
                                              TCATAATACTGCCGAATTCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAA                                                 <  SRR4302543.3430215/94‑1 (MQ=60)
                                               CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                >  SRR4302543.7750214/1‑94 (MQ=60)
                                               CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                <  SRR4302543.7647456/94‑1 (MQ=60)
                                               CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                >  SRR4302543.7033767/1‑94 (MQ=60)
                                               CATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAA                                                >  SRR4302543.5104734/1‑94 (MQ=60)
                                                ATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGTCTGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGCGTTAACAAAAA                                               >  SRR4302543.2112372/1‑94 (MQ=60)
                                                ATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAA                                               <  SRR4302543.3960890/94‑1 (MQ=60)
                                                  AATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAACCG                                             >  SRR4302543.5746829/1‑94 (MQ=60)
                                                  AATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACG                                             <  SRR4302543.5678148/94‑1 (MQ=60)
                                                  AATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACA                                             >  SRR4302543.5746830/1‑94 (MQ=60)
                                                     ACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATG                                          >  SRR4302543.3047466/1‑94 (MQ=60)
                                                     ACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATG                                          <  SRR4302543.7016832/94‑1 (MQ=60)
                                                      CTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGT                                         >  SRR4302543.414519/1‑94 (MQ=60)
                                                      CTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGT                                         <  SRR4302543.595911/94‑1 (MQ=60)
                                                      CTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGA                                         >  SRR4302543.2699445/1‑94 (MQ=60)
                                                       TGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTA                                        <  SRR4302543.106874/94‑1 (MQ=60)
                                                       TGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGATGGTATCCATGCCAGACAGGCCAAGAATCTGCGGGCTATCAAATACGATGTA                                        >  SRR4302543.7044574/1‑94 (MQ=53)
                                                        GCCGAATGCAGTGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAA                                       <  SRR4302543.6055598/94‑1 (MQ=60)
                                                        GCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAA                                       <  SRR4302543.3610659/94‑1 (MQ=60)
                                                        GCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAA                                       >  SRR4302543.2491463/1‑94 (MQ=60)
                                                         CCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATACCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAA                                      >  SRR4302543.6375247/1‑94 (MQ=60)
                                                          CGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAC                                     <  SRR4302543.765134/94‑1 (MQ=60)
                                                           GAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACC                                    >  SRR4302543.2779870/1‑94 (MQ=60)
                                                           GAACGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACC                                    <  SRR4302543.4747938/94‑1 (MQ=60)
                                                            AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                   >  SRR4302543.6963776/1‑94 (MQ=60)
                                                            AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                   <  SRR4302543.4119115/94‑1 (MQ=60)
                                                            AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                   <  SRR4302543.4956885/94‑1 (MQ=60)
                                                             ATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAT                                  >  SRR4302543.6488691/1‑94 (MQ=60)
                                                             ATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAT                                  <  SRR4302543.4628964/94‑1 (MQ=60)
                                                             ATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAT                                  >  SRR4302543.50621/1‑94 (MQ=60)
                                                             ATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAT                                  >  SRR4302543.7650752/1‑94 (MQ=60)
                                                              TGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATC                                 <  SRR4302543.599613/94‑1 (MQ=60)
                                                              TGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATC                                 <  SRR4302543.7918970/94‑1 (MQ=60)
                                                              TGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATC                                 <  SRR4302543.7961023/94‑1 (MQ=60)
                                                               GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                                <  SRR4302543.5848075/94‑1 (MQ=60)
                                                               GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                                <  SRR4302543.1424982/94‑1 (MQ=60)
                                                               GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                                <  SRR4302543.5439532/94‑1 (MQ=60)
                                                               GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                                >  SRR4302543.566236/1‑94 (MQ=60)
                                                                CAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGT                               <  SRR4302543.2254559/94‑1 (MQ=60)
                                                                CAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGT                               >  SRR4302543.4598595/1‑94 (MQ=60)
                                                                 AGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTC                              >  SRR4302543.3188671/1‑94 (MQ=60)
                                                                 AGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTC                              >  SRR4302543.7166169/1‑94 (MQ=60)
                                                                  GCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCA                             <  SRR4302543.2748709/94‑1 (MQ=60)
                                                                  GCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCA                             <  SRR4302543.7948243/94‑1 (MQ=60)
                                                                   CGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAG                            <  SRR4302543.7335678/94‑1 (MQ=60)
                                                                   CGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAG                            >  SRR4302543.1332000/1‑94 (MQ=60)
                                                                   CGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAG                            >  SRR4302543.5816662/1‑94 (MQ=60)
                                                                    GATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGG                           >  SRR4302543.1805971/1‑94 (MQ=60)
                                                                    GATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGG                           <  SRR4302543.6945025/94‑1 (MQ=60)
                                                                    GATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAGCGTCAGG                           <  SRR4302543.1417279/94‑1 (MQ=60)
                                                                    GATCAGACAGATAGTGACAAAGACGTCGCTAGAATCCATGCCAGCAACGCCAAGAAATTGCAAGTAAAAAAAAACGATGTAAAACATCGTAAGG                           <  SRR4302543.7076707/94‑1 (MQ=25)
                                                                     ATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGA                          >  SRR4302543.777898/1‑94 (MQ=60)
                                                                     ATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATAGTCAGGA                          >  SRR4302543.606924/1‑94 (MQ=60)
                                                                      TCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAA                         <  SRR4302543.246352/94‑1 (MQ=60)
                                                                       CAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAG                        <  SRR4302543.7901359/94‑1 (MQ=60)
                                                                       CAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAG                        <  SRR4302543.6094118/94‑1 (MQ=60)
                                                                       CAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAG                        <  SRR4302543.5523797/94‑1 (MQ=60)
                                                                        AGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGG                       <  SRR4302543.3282312/94‑1 (MQ=60)
                                                                        AGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGG                       >  SRR4302543.7369282/1‑94 (MQ=60)
                                                                        AGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGG                       >  SRR4302543.5836718/1‑94 (MQ=60)
                                                                          ACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG                     <  SRR4302543.4710754/94‑1 (MQ=60)
                                                                          ACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG                     <  SRR4302543.20389/94‑1 (MQ=60)
                                                                          ACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG                     <  SRR4302543.4104474/94‑1 (MQ=60)
                                                                          ACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG                     <  SRR4302543.7786135/94‑1 (MQ=60)
                                                                           CAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGG                    <  SRR4302543.2659285/94‑1 (MQ=60)
                                                                           CAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGG                    <  SRR4302543.2981865/94‑1 (MQ=60)
                                                                           CAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGG                    >  SRR4302543.7879785/1‑94 (MQ=60)
                                                                            AGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGT                   <  SRR4302543.6806458/94‑1 (MQ=60)
                                                                            AGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGT                   <  SRR4302543.1228746/94‑1 (MQ=60)
                                                                            AGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGT                   >  SRR4302543.3882934/1‑94 (MQ=60)
                                                                               TAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAA                >  SRR4302543.3749387/1‑94 (MQ=60)
                                                                                 GTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAC              <  SRR4302543.7857559/94‑1 (MQ=60)
                                                                                 GTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAC              <  SRR4302543.7475436/94‑1 (MQ=60)
                                                                                 GTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAC              <  SRR4302543.2813375/94‑1 (MQ=60)
                                                                                 GTGAAGAAGACGTCGCGGGTATCCAGCCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAC              <  SRR4302543.5282281/94‑1 (MQ=60)
                                                                                  TGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACC             >  SRR4302543.3341764/1‑94 (MQ=60)
                                                                                  TGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACC             >  SRR4302543.7762796/1‑94 (MQ=60)
                                                                                   GAGGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG            <  SRR4302543.1796764/94‑1 (MQ=60)
                                                                                   GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG            >  SRR4302543.7758087/1‑94 (MQ=60)
                                                                                   GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG            <  SRR4302543.4858250/94‑1 (MQ=60)
                                                                                   GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG            <  SRR4302543.7712946/94‑1 (MQ=60)
                                                                                   GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG            <  SRR4302543.62494/94‑1 (MQ=60)
                                                                                     CGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGC          >  SRR4302543.66808/1‑94 (MQ=60)
                                                                                     CGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGC          <  SRR4302543.6967606/94‑1 (MQ=60)
                                                                                      GAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCG         >  SRR4302543.1634/1‑94 (MQ=60)
                                                                                      GAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCG         >  SRR4302543.7415789/1‑94 (MQ=60)
                                                                                      GAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCG         >  SRR4302543.3501657/1‑94 (MQ=60)
                                                                                      GAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCG         >  SRR4302543.3461291/1‑94 (MQ=60)
                                                                                      GAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCG         >  SRR4302543.6827857/1‑94 (MQ=60)
                                                                                       AAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTACAACCGGCGA        >  SRR4302543.2376300/1‑94 (MQ=60)
                                                                                       AAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGA        <  SRR4302543.2539219/94‑1 (MQ=60)
                                                                                        AGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGAT       <  SRR4302543.7557604/94‑1 (MQ=60)
                                                                                        AGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGAT       <  SRR4302543.7504601/94‑1 (MQ=60)
                                                                                         GACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC      >  SRR4302543.3709740/1‑94 (MQ=60)
                                                                                         GACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC      <  SRR4302543.425577/94‑1 (MQ=60)
                                                                                         GACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC      <  SRR4302543.4947715/94‑1 (MQ=60)
                                                                                          ACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCA     >  SRR4302543.1635307/1‑94 (MQ=60)
                                                                                           CGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCAC    <  SRR4302543.689901/94‑1 (MQ=60)
                                                                                           CGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCAC    >  SRR4302543.4416467/1‑94 (MQ=60)
                                                                                           CGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCAC    <  SRR4302543.435484/94‑1 (MQ=60)
                                                                                            GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT   <  SRR4302543.1381472/94‑1 (MQ=60)
                                                                                            GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT   >  SRR4302543.13010/1‑94 (MQ=60)
                                                                                            GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT   >  SRR4302543.4118295/1‑94 (MQ=60)
                                                                                             TCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACTT  <  SRR4302543.3161611/94‑1 (MQ=60)
                                                                                             TCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACTT  >  SRR4302543.3590045/1‑94 (MQ=60)
                                                                                             TCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACTT  <  SRR4302543.5554515/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACTT  >  NC_000913/3893961‑3894147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑