Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,894,054	G→T	A62D (GCC→GAC)	yieG ←	putative membrane/transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,894,054	0	G	T	100.0%	890.4 / NA	248	A62D (GCC→GAC)	yieG	putative membrane/transport protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (132/116); total (132/116)

Only 100 of 261 total aligned reads displayed.

ATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC  >  NC_000913/3893964‑3894143

aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcg                                                                                         >  1:1874634/1‑93 (MQ=255)

 tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                                                                                        <  1:33141/93‑1 (MQ=255)

 tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                                                                                        >  1:76492/1‑93 (MQ=255)

 tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                                                                                        >  1:2278080/1‑93 (MQ=255)

   gCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTg                                                                                      >  1:2138845/1‑93 (MQ=255)

       gTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTAt                                                                                  <  2:4022932/93‑1 (MQ=255)

        tGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATc                                                                                 <  2:1954887/93‑1 (MQ=255)

          ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                                                                               <  1:3942390/93‑1 (MQ=255)

           cAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGGATCCAt                                                                              >  1:3580258/1‑93 (MQ=255)

            aGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATg                                                                             >  1:3967968/1‑93 (MQ=255)

            aGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATg                                                                             <  2:337813/93‑1 (MQ=255)

            aGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATg                                                                             <  1:82159/93‑1 (MQ=255)

             gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCGATGc                                                                            >  2:873194/1‑93 (MQ=255)

             gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGc                                                                            >  1:1747782/1‑93 (MQ=255)

             gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGc                                                                            >  2:4284985/1‑93 (MQ=255)

                 aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc                                                                        <  2:4171264/93‑1 (MQ=255)

                     ggCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACg                                                                    >  1:181911/1‑93 (MQ=255)

                     ggCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACg                                                                    >  2:4104911/1‑93 (MQ=255)

                        aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa                                                                 >  2:547950/1‑93 (MQ=255)

                        aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa                                                                 >  2:1146201/1‑93 (MQ=255)

                         ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa                                                                <  2:292189/93‑1 (MQ=255)

                         ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa                                                                <  1:1798117/93‑1 (MQ=255)

                           ttggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGa                                                              >  2:2026845/4‑93 (MQ=255)

                           ttggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGa                                                              <  1:2245935/90‑1 (MQ=255)

                           ttggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGa                                                              >  1:2010852/4‑93 (MQ=255)

                            tggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGaa                                                             <  2:2853600/91‑1 (MQ=255)

                            tggCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGaa                                                             <  2:4665032/91‑1 (MQ=255)

                              gCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAtt                                                           >  1:2962811/1‑93 (MQ=255)

                              gCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAtt                                                           <  2:3171582/93‑1 (MQ=255)

                               cAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAttt                                                          <  1:3530897/93‑1 (MQ=255)

                                aaaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTg                                                         >  1:2745459/1‑93 (MQ=255)

                                 aaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGc                                                        >  2:1634074/1‑93 (MQ=255)

                                 aaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGc                                                        <  2:132371/93‑1 (MQ=255)

                                 aaCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGc                                                        >  1:2820534/1‑93 (MQ=255)

                                  aCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCg                                                       >  1:1163934/1‑93 (MQ=255)

                                  aCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCg                                                       <  2:3043419/93‑1 (MQ=255)

                                  aCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCg                                                       <  2:1620545/93‑1 (MQ=255)

                                  aCAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCg                                                       >  2:3266963/1‑93 (MQ=255)

                                    aGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCggg                                                     >  2:387848/1‑93 (MQ=255)

                                    aGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCggg                                                     <  1:3107677/93‑1 (MQ=255)

                                     gTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGt                                                    >  2:4518393/1‑93 (MQ=255)

                                     gTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGt                                                    >  2:1483262/1‑93 (MQ=255)

                                        ccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTaa                                                 >  2:2520455/1‑93 (MQ=255)

                                        ccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTaa                                                 >  1:253671/1‑93 (MQ=255)

                                        ccATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTaa                                                 <  1:167046/93‑1 (MQ=255)

                                         catcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAAc                                                <  1:3246680/93‑1 (MQ=255)

                                         catcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAAc                                                >  1:1247108/1‑93 (MQ=255)

                                          atcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACa                                               <  1:11967/93‑1 (MQ=255)

                                           tcatAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGACAGCAACGCCAAGAATTTGCGGGTTAACaa                                              >  2:2693178/1‑93 (MQ=255)

                                              taataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaaaa                                           >  2:4165688/1‑93 (MQ=255)

                                              taataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaaaa                                           >  1:4179833/1‑93 (MQ=255)

                                               aataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAAc                                          >  1:1556222/1‑93 (MQ=255)

                                               aataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAAc                                          >  2:2806735/1‑93 (MQ=255)

                                               aataCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAAc                                          <  1:1122258/93‑1 (MQ=255)

                                                 taCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGa                                        <  1:734533/93‑1 (MQ=255)

                                                 taCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGa                                        >  2:3460917/1‑93 (MQ=255)

                                                   cTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATg                                      <  2:1850508/93‑1 (MQ=255)

                                                     gCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTa                                    <  1:2430078/93‑1 (MQ=255)

                                                      ccGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaa                                   <  2:2613871/93‑1 (MQ=255)

                                                       cGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaaa                                  >  1:1452830/1‑93 (MQ=255)

                                                       cGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaaa                                  >  1:4067792/1‑93 (MQ=255)

                                                       cGAATGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACACAAACGATGTaaa                                  >  2:577400/1‑93 (MQ=255)

                                                        gAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAc                                 >  2:3146498/1‑93 (MQ=255)

                                                          aTGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCa                               >  1:3793515/1‑93 (MQ=255)

                                                           tGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAt                              <  2:688197/93‑1 (MQ=255)

                                                            gCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATc                             <  1:1412449/93‑1 (MQ=255)

                                                             cAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCg                            <  2:1750374/93‑1 (MQ=255)

                                                              aGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGt                           <  2:6608/93‑1 (MQ=255)

                                                              aGCGATCAGACAGGTAGAGACGAAGACGTGGCTGGTATCCATGCCAGCAACGACAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCgc                           >  2:2131750/1‑92 (MQ=255)

                                                                 gATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAg                        <  2:2348550/93‑1 (MQ=255)

                                                                 gATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGGTAACAAAAACGATGTAAACCATCGTCAg                        >  1:560402/1‑93 (MQ=255)

                                                                   tCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGa                      >  2:1430116/1‑93 (MQ=255)

                                                                   tCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGa                      <  1:3786665/93‑1 (MQ=255)

                                                                   tCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGa                      >  1:2606229/1‑93 (MQ=255)

                                                                    cAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGaa                     <  1:2880486/93‑1 (MQ=255)

                                                                    cAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGaa                     >  2:3720685/1‑93 (MQ=255)

                                                                     aGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAg                    <  1:1309299/93‑1 (MQ=255)

                                                                      gACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAgg                   >  2:4302612/1‑93 (MQ=255)

                                                                      gACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAgg                   <  2:306690/93‑1 (MQ=255)

                                                                       aCAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggt                  <  1:3407435/93‑1 (MQ=255)

                                                                         aGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgg                <  2:3240088/93‑1 (MQ=255)

                                                                         aGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtgg                >  1:4360761/1‑93 (MQ=255)

                                                                          ggTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtggt               >  1:3342860/1‑93 (MQ=255)

                                                                              gTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTaaaa           >  2:3559334/1‑93 (MQ=255)

                                                                              gTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTaaaa           >  2:741296/1‑93 (MQ=255)

                                                                              gTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTaaaa           <  1:4109125/93‑1 (MQ=255)

                                                                               tGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAc          >  2:1225691/1‑93 (MQ=255)

                                                                                gACGAAGACGGCGCTAGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAcc         <  2:3744753/93‑1 (MQ=255)

                                                                                 aCGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCg        <  2:3487088/93‑1 (MQ=255)

                                                                                 aCGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCg        <  2:2278080/93‑1 (MQ=255)

                                                                                  cGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCgg       >  2:2317870/1‑93 (MQ=255)

                                                                                   ggaGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACACTCGTCAGGAAGGTGGTAAAACCGGc      >  1:4529475/3‑93 (MQ=255)

                                                                                   gAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGc      >  1:4649580/1‑93 (MQ=255)

                                                                                   gAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGc      >  2:3581788/1‑93 (MQ=255)

                                                                                    aaGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCg     >  2:3432022/1‑93 (MQ=255)

                                                                                     aGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGa    >  2:477896/1‑93 (MQ=255)

                                                                                     aGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGa    <  1:623053/93‑1 (MQ=255)

                                                                                     aGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGa    >  1:1065519/1‑93 (MQ=255)

                                                                                      gACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGAt   >  2:3805892/1‑93 (MQ=255)

                                                                                       aCGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATc  >  1:1031201/1‑93 (MQ=255)

ATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC  >  NC_000913/3893964‑3894143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT  >  NC_000913/3893961‑3894146

CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                                                                              >  SRR4302535.4011751/1‑94 (MQ=60)

  CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                                                                            >  SRR4302535.1888139/1‑94 (MQ=60)

  CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCG                                                                                            >  SRR4302535.1800519/1‑94 (MQ=60)

   ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                                                                                           >  SRR4302535.77046/1‑94 (MQ=60)

   ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                                                                                           >  SRR4302535.2294352/1‑94 (MQ=60)

   ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                                                                                           >  SRR4302535.685395/1‑94 (MQ=60)

    TAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCT                                                                                          <  SRR4302535.33396/94‑1 (MQ=60)

     AGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTG                                                                                         >  SRR4302535.2154247/1‑94 (MQ=60)

     AGCGGGGGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATTCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTG                                                                                         <  SRR4302535.1065520/94‑1 (MQ=60)

      GCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGG                                                                                        >  SRR4302535.1089803/1‑94 (MQ=60)

        GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                                                                                      >  SRR4302535.3679021/1‑94 (MQ=60)

          GTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATC                                                                                    >  SRR4302535.1649429/1‑94 (MQ=60)

          GTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATC                                                                                    >  SRR4302535.2285830/1‑94 (MQ=60)

           TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                                                                                   <  SRR4302535.4443865/94‑1 (MQ=60)

           TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                                                                                   >  SRR4302535.4129299/1‑94 (MQ=60)

            GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                                                                                  >  SRR4302535.4195696/1‑94 (MQ=60)

             TCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                 <  SRR4302535.178617/94‑1 (MQ=60)

             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                 <  SRR4302535.2240336/94‑1 (MQ=60)

             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                 >  SRR4302535.1825142/1‑94 (MQ=60)

             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                 <  SRR4302535.3953470/94‑1 (MQ=60)

             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                                                                                 <  SRR4302535.3969300/94‑1 (MQ=60)

             CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGGATCCAT                                                                                 >  SRR4302535.3605071/1‑94 (MQ=60)

              CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                                                                                >  SRR4302535.1343471/1‑94 (MQ=60)

              CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                                                                                >  SRR4302535.3995071/1‑94 (MQ=60)

               AGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGC                                                                               >  SRR4302535.1760424/1‑94 (MQ=60)

               AGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGC                                                                               <  SRR4302535.82743/94‑1 (MQ=60)

                   CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC                                                                           >  SRR4302535.2522775/1‑94 (MQ=60)

                   CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC                                                                           >  SRR4302535.4542931/1‑94 (MQ=60)

                    AACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA                                                                          <  SRR4302535.1369865/94‑1 (MQ=60)

                      CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                        <  SRR4302535.1204654/94‑1 (MQ=60)

                      CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC                                                                        >  SRR4302535.618447/1‑94 (MQ=60)

                       TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG                                                                       >  SRR4302535.183170/1‑94 (MQ=60)

                           AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA                                                                   <  SRR4302535.2911119/94‑1 (MQ=60)

                            GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG                                                                  <  SRR4302535.2631700/94‑1 (MQ=60)

                            GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG                                                                  <  SRR4302535.1811119/94‑1 (MQ=60)

                             GTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGA                                                                 >  SRR4302535.2025328/1‑94 (MQ=60)

                              TTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAA                                                                <  SRR4302535.935454/94‑1 (MQ=60)

                              TTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAA                                                                <  SRR4302535.2261905/94‑1 (MQ=60)

                               TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT                                                               <  SRR4302535.47098/94‑1 (MQ=60)

                               TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT                                                               <  SRR4302535.4105866/94‑1 (MQ=60)

                                GGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT                                                              >  SRR4302535.1965272/1‑94 (MQ=60)

                                GGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT                                                              >  SRR4302535.2983609/1‑94 (MQ=60)

                                GGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT                                                              <  SRR4302535.3406505/94‑1 (MQ=60)

                                 GCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCACTGGTATCCATGCCAGCAACGCCAAGAATTT                                                             >  SRR4302535.762750/1‑94 (MQ=60)

                                  CAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTG                                                            >  SRR4302535.2765026/1‑94 (MQ=60)

                                  CAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTG                                                            <  SRR4302535.3555411/94‑1 (MQ=60)

                                   AAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGC                                                           >  SRR4302535.2840567/1‑94 (MQ=60)

                                    AACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                          <  SRR4302535.1246223/94‑1 (MQ=60)

                                    AACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                          >  SRR4302535.3600619/1‑94 (MQ=60)

                                    AACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                          <  SRR4302535.4277849/94‑1 (MQ=60)

                                    AACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCG                                                          >  SRR4302535.1172246/1‑94 (MQ=60)

                                     ACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGG                                                         <  SRR4302535.1478986/94‑1 (MQ=60)

                                      CAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGG                                                        <  SRR4302535.1198031/94‑1 (MQ=60)

                                      CAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGG                                                        <  SRR4302535.2964234/94‑1 (MQ=60)

                                       AGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGT                                                       >  SRR4302535.4054195/1‑94 (MQ=60)

                                       AGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGT                                                       >  SRR4302535.4340824/1‑94 (MQ=60)

                                       AGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGT                                                       <  SRR4302535.3129313/94‑1 (MQ=60)

                                         TCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTA                                                     <  SRR4302535.3884115/94‑1 (MQ=60)

                                          CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                    <  SRR4302535.3175482/94‑1 (MQ=60)

                                          CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                    >  SRR4302535.3493921/1‑94 (MQ=60)

                                          CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                    >  SRR4302535.2615495/1‑94 (MQ=60)

                                          CCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAA                                                    >  SRR4302535.255423/1‑94 (MQ=60)

                                           CCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAAC                                                   <  SRR4302535.881837/94‑1 (MQ=60)

                                           CCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAAC                                                   >  SRR4302535.1256005/1‑94 (MQ=60)

                                           CCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAAC                                                   >  SRR4302535.184668/1‑94 (MQ=60)

                                           CCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAAC                                                   <  SRR4302535.168207/94‑1 (MQ=60)

                                            CATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACA                                                  >  SRR4302535.1845709/1‑94 (MQ=60)

                                            CATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACA                                                  <  SRR4302535.1539071/94‑1 (MQ=60)

                                            CATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACA                                                  <  SRR4302535.3269228/94‑1 (MQ=60)

                                             ATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAA                                                 <  SRR4302535.1075630/94‑1 (MQ=60)

                                             ATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAA                                                 <  SRR4302535.12058/94‑1 (MQ=60)

                                             ATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAA                                                 >  SRR4302535.546605/1‑94 (MQ=60)

                                                ATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAA                                              >  SRR4302535.4208440/1‑94 (MQ=60)

                                                ATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAA                                              <  SRR4302535.407357/94‑1 (MQ=60)

                                                 TAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAAC                                             >  SRR4302535.1567442/1‑94 (MQ=60)

                                                  AATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACG                                            >  SRR4302535.2788989/1‑94 (MQ=60)

                                                  AATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACG                                            <  SRR4302535.1130265/94‑1 (MQ=60)

                                                    TACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGAT                                          <  SRR4302535.739697/94‑1 (MQ=60)

                                                     ACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATG                                         <  SRR4302535.337345/94‑1 (MQ=60)

                                                     ACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATG                                         <  SRR4302535.1574606/94‑1 (MQ=60)

                                                     ACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATG                                         <  SRR4302535.824177/94‑1 (MQ=60)

                                                        GCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAA                                      <  SRR4302535.2447586/94‑1 (MQ=60)

                                                         CCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAA                                     >  SRR4302535.1463228/1‑94 (MQ=60)

                                                         CCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAA                                     >  SRR4302535.4095626/1‑94 (MQ=60)

                                                          CGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAAC                                    >  SRR4302535.362202/1‑94 (MQ=60)

                                                           GAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACC                                   <  SRR4302535.1699153/94‑1 (MQ=60)

                                                            AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAACACGATGTAAACCA                                  >  SRR4302535.3298792/1‑94 (MQ=60)

                                                            AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                  >  SRR4302535.3819669/1‑94 (MQ=60)

                                                            AATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                  <  SRR4302535.3366030/94‑1 (MQ=60)

                                                            AATGCAGCGATCAGACAGGGAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCA                                  >  SRR4302535.2402425/1‑94 (MQ=60)

                                                             ATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAT                                 <  SRR4302535.1280906/94‑1 (MQ=60)

                                                              TGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATC                                >  SRR4302535.3255043/1‑94 (MQ=60)

                                                              TGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATC                                <  SRR4302535.3044381/94‑1 (MQ=60)

                                                               GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                               <  SRR4302535.1422532/94‑1 (MQ=60)

                                                               GCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCG                               <  SRR4302535.3277209/94‑1 (MQ=60)

                                                                CAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGT                              <  SRR4302535.2749921/94‑1 (MQ=60)

                                                                 AGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTC                             >  SRR4302535.1171843/1‑94 (MQ=60)

                                                                  GCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCA                            <  SRR4302535.688904/94‑1 (MQ=60)

                                                                   CGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAG                           >  SRR4302535.1993461/1‑94 (MQ=60)

                                                                   CGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGGTAACAAAAACGATGTAAACCATCGTCAG                           >  SRR4302535.564321/1‑94 (MQ=60)

                                                                    GATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGG                          >  SRR4302535.2450149/1‑94 (MQ=60)

                                                                    GATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGG                          >  SRR4302535.23406/1‑94 (MQ=60)

                                                                     ATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGA                         >  SRR4302535.2624932/1‑94 (MQ=60)

                                                                      TCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAA                        <  SRR4302535.3812785/94‑1 (MQ=60)

                                                                       CAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAG                       <  SRR4302535.2900877/94‑1 (MQ=60)

                                                                        AGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGG                      <  SRR4302535.1318656/94‑1 (MQ=60)

                                                                         GACAGGTAGTGACTAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGT                     <  SRR4302535.1428535/94‑1 (MQ=60)

                                                                          ACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG                    <  SRR4302535.3431152/94‑1 (MQ=60)

                                                                           CAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGG                   >  SRR4302535.4390606/1‑94 (MQ=60)

                                                                           CAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGG                   >  SRR4302535.3814049/1‑94 (MQ=60)

                                                                            AGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGT                  >  SRR4302535.3366152/1‑94 (MQ=60)

                                                                                 GTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAAC             <  SRR4302535.4137257/94‑1 (MQ=60)

                                                                                   GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG           <  SRR4302535.276880/94‑1 (MQ=60)

                                                                                   GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG           <  SRR4302535.2854450/94‑1 (MQ=60)

                                                                                   GACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCG           >  SRR4302535.3569548/1‑94 (MQ=60)

                                                                                    ACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGG          <  SRR4302535.122701/94‑1 (MQ=60)

                                                                                    ACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGG          <  SRR4302535.2103135/94‑1 (MQ=60)

                                                                                     CGGAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACACTCGTCAGGAAGGTGGTAAAACCGGC         >  SRR4302535.4560487/1‑94 (MQ=60)

                                                                                     CGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGC         >  SRR4302535.4681070/1‑94 (MQ=60)

                                                                                     CGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGC         >  SRR4302535.3994650/1‑94 (MQ=60)

                                                                                       AAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGA       >  SRR4302535.2177784/1‑94 (MQ=60)

                                                                                       AAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGA       >  SRR4302535.1073099/1‑94 (MQ=60)

                                                                                        AGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGAT      <  SRR4302535.627382/94‑1 (MQ=60)

                                                                                         GACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC     <  SRR4302535.4588125/94‑1 (MQ=60)

                                                                                         GACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATC     >  SRR4302535.1038525/1‑94 (MQ=60)

                                                                                          ACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCA    <  SRR4302535.742798/94‑1 (MQ=60)

                                                                                           CGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCAC   <  SRR4302535.3220027/94‑1 (MQ=60)

                                                                                           CGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCAC   >  SRR4302535.2510095/1‑94 (MQ=60)

                                                                                           CGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCAC   >  SRR4302535.1920286/1‑94 (MQ=60)

                                                                                           CGTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCAC   <  SRR4302535.2647821/94‑1 (MQ=60)

                                                                                            GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT  >  SRR4302535.4399724/1‑94 (MQ=60)

                                                                                            GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT  >  SRR4302535.4108512/1‑94 (MQ=60)

                                                                                            GTCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT  <  SRR4302535.3795324/94‑1 (MQ=60)

CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTGGTAAAACCGGCGATCACT  >  NC_000913/3893961‑3894146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`