Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,893,993 A→G A82A (GCT→GCC)  yieG ← putative membrane/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,893,9930AG100.0% 787.3 / NA 244A82A (GCT→GCC) yieGputative membrane/transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (130/114);  total (130/114)

Only 100 of 249 total aligned reads displayed.

GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAA  >  NC_000913/3893903‑3894081
                                                                                          |                                                                                        
gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGc                                                                                        <  2:949032/93‑1 (MQ=255)
gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGc                                                                                        <  1:3576839/93‑1 (MQ=255)
 aCCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCa                                                                                       >  2:3928244/1‑93 (MQ=255)
  ccTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCaa                                                                                      <  2:1149955/93‑1 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                   <  2:4440179/93‑1 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                   <  1:3910859/93‑1 (MQ=255)
     gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACa                                                                                   <  1:1635103/93‑1 (MQ=255)
        aCGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTc                                                                                <  2:3484063/93‑1 (MQ=255)
         cGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTcc                                                                               <  2:2028912/93‑1 (MQ=255)
           gCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCca                                                                             <  2:18122/93‑1 (MQ=255)
           gCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCca                                                                             <  1:455494/93‑1 (MQ=255)
           gCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCca                                                                             <  2:3759879/93‑1 (MQ=255)
           gCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTGGCAAACAGTCCca                                                                             >  2:3035881/1‑93 (MQ=255)
            cAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcat                                                                            <  2:428001/93‑1 (MQ=255)
             aaGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCcatc                                                                           <  2:1676119/93‑1 (MQ=255)
                 ccATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataa                                                                       >  1:719496/1‑93 (MQ=255)
                 ccATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataa                                                                       <  1:2526879/93‑1 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                      >  1:931472/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                      >  1:4199128/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                      >  1:307125/1‑93 (MQ=255)
                  cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCataat                                                                      >  1:1851565/1‑93 (MQ=255)
                    tCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAc                                                                    <  2:2758020/93‑1 (MQ=255)
                    tCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAc                                                                    <  2:1246572/93‑1 (MQ=255)
                    tCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAc                                                                    >  1:3398823/1‑93 (MQ=255)
                     cGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACt                                                                   <  1:2420770/93‑1 (MQ=255)
                       ccTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGc                                                                 >  1:140892/1‑93 (MQ=255)
                         tGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCg                                                               >  2:1428941/1‑93 (MQ=255)
                         tGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCg                                                               >  1:443193/1‑93 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTTCCGaa                                                             <  2:2686280/93‑1 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGaa                                                             <  1:409806/93‑1 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGaa                                                             <  1:324929/93‑1 (MQ=255)
                           tACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGaa                                                             >  1:3262197/1‑93 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                            <  1:836710/93‑1 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                            <  1:2097780/93‑1 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                            <  2:63045/93‑1 (MQ=255)
                            aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAt                                                            <  2:1313922/93‑1 (MQ=255)
                              aaCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGc                                                          >  2:1979466/1‑93 (MQ=255)
                              aaCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGc                                                          <  1:1032077/93‑1 (MQ=255)
                               aCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCa                                                         >  2:4607963/1‑93 (MQ=255)
                               aCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCa                                                         <  2:3095490/93‑1 (MQ=255)
                               aCGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCa                                                         <  2:650290/93‑1 (MQ=255)
                                cGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAg                                                        >  2:2603773/1‑93 (MQ=255)
                                cGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGGGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAg                                                        >  1:201298/1‑93 (MQ=255)
                                 gACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGc                                                       >  2:4408512/1‑93 (MQ=255)
                                 gACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGc                                                       <  2:7457/93‑1 (MQ=255)
                                 gACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGc                                                       >  2:1481358/1‑93 (MQ=255)
                                  aCAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCg                                                      >  2:4662363/1‑93 (MQ=255)
                                  aCAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCg                                                      <  2:1083086/93‑1 (MQ=255)
                                   cAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGa                                                     >  1:3961454/1‑93 (MQ=255)
                                   cAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGa                                                     >  2:3479912/1‑93 (MQ=255)
                                   cAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGa                                                     >  1:3946585/1‑93 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                   <  1:1887289/93‑1 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                   <  2:1936705/93‑1 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                   >  2:1263115/1‑93 (MQ=255)
                                     aaaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                   >  1:2480554/1‑93 (MQ=255)
                                      aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCa                                                  >  1:1481311/1‑93 (MQ=255)
                                      aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCa                                                  <  2:2379903/93‑1 (MQ=255)
                                      aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCa                                                  >  1:1983429/1‑93 (MQ=255)
                                      aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCa                                                  <  1:12063/93‑1 (MQ=255)
                                       aGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAg                                                 <  1:2972798/93‑1 (MQ=255)
                                       aGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAg                                                 >  2:2172633/1‑93 (MQ=255)
                                          gaagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACa                                              >  2:3551632/1‑93 (MQ=255)
                                            agaaCTCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAgg                                            <  2:1916060/93‑1 (MQ=255)
                                            agaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAgg                                            >  2:409326/1‑93 (MQ=255)
                                                 gCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTg                                       <  2:3189718/93‑1 (MQ=255)
                                                 gCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTg                                       >  2:4473787/1‑93 (MQ=255)
                                                 gCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTg                                       >  1:2379494/1‑93 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                      >  1:470347/1‑93 (MQ=255)
                                                  cATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGa                                      <  1:3739502/93‑1 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                    >  2:1559313/1‑93 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                    >  1:2747948/1‑93 (MQ=255)
                                                    ttCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGCGACg                                    >  1:3124735/1‑93 (MQ=255)
                                                     tCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGa                                   >  1:2810937/1‑93 (MQ=255)
                                                         gCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAc                               <  1:795988/93‑1 (MQ=255)
                                                            cATAGCGGGGGCCAGAGCAACTGGCAGGTTGGCAAACAGTCCCATCATACTACTGCCGAATGCAGCGATCAGACAGGTAGAGACGAAGACgtg                            >  2:1366577/1‑91 (MQ=255)
                                                            cATAGCGGGAGCCAGTGCAACTGGCAGGGTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACgtg                            >  2:3954612/1‑91 (MQ=255)
                                                             aTAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGgcg                           >  1:1787589/1‑93 (MQ=255)
                                                              tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                          >  2:1625845/1‑93 (MQ=255)
                                                              tAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGtcgc                          <  1:33141/93‑1 (MQ=255)
                                                               aGCGGGGGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATTCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCt                         <  1:1057995/93‑1 (MQ=255)
                                                                 cGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTgg                       >  1:1082110/1‑93 (MQ=255)
                                                                   ggTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTa                     <  2:2252650/93‑1 (MQ=255)
                                                                   ggTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTa                     >  1:3653724/1‑93 (MQ=255)
                                                                     tGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATc                   >  1:2269647/1‑93 (MQ=255)
                                                                      gCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATcc                  >  1:4101220/1‑93 (MQ=255)
                                                                       ccAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCa                 <  1:2224504/93‑1 (MQ=255)
                                                                         aGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATg               <  2:32394/93‑1 (MQ=255)
                                                                         aGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATg               <  2:337813/93‑1 (MQ=255)
                                                                          gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCGATGc              >  2:873194/1‑93 (MQ=255)
                                                                          gTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGc              >  1:1747782/1‑93 (MQ=255)
                                                                            gCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCa            <  2:1183943/93‑1 (MQ=255)
                                                                              aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc          >  1:4511986/1‑93 (MQ=255)
                                                                              aaCTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGc          <  1:1360138/93‑1 (MQ=255)
                                                                                cTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCaa        <  1:1196085/93‑1 (MQ=255)
                                                                                cTGGCAGGTTGGAAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCaa        <  2:3420972/93‑1 (MQ=255)
                                                                                 tGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAc       >  1:614178/1‑93 (MQ=255)
                                                                                  ggCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACg      >  2:4104911/1‑93 (MQ=255)
                                                                                  ggCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACg      >  1:181911/1‑93 (MQ=255)
                                                                                     aGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCa   >  2:547950/1‑93 (MQ=255)
                                                                                      ggTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCaa  <  1:2612959/93‑1 (MQ=255)
                                                                                          |                                                                                        
GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAA  >  NC_000913/3893903‑3894081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATT  >  NC_000913/3893900‑3894086
                                                                                             |                                                                                             
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTG                                                                                               >  SRR4302535.6664/1‑94 (MQ=60)
 CCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGG                                                                                              >  SRR4302535.253126/1‑94 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                             <  SRR4302535.2494199/94‑1 (MQ=60)
  CGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGC                                                                                             >  SRR4302535.304346/1‑94 (MQ=60)
   GACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCA                                                                                            <  SRR4302535.3601636/94‑1 (MQ=60)
    ACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAA                                                                                           >  SRR4302535.3694917/1‑94 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302535.2403480/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302535.244058/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302535.3297791/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302535.1646918/94‑1 (MQ=60)
        GCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAG                                                                                       <  SRR4302535.3937636/94‑1 (MQ=60)
         CCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGT                                                                                      <  SRR4302535.4506694/94‑1 (MQ=60)
             GGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCA                                                                                  >  SRR4302535.3275196/1‑94 (MQ=60)
              GCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCGATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCAT                                                                                 <  SRR4302535.458682/94‑1 (MQ=60)
              GCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCAT                                                                                 <  SRR4302535.458681/94‑1 (MQ=60)
              GCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCAT                                                                                 <  SRR4302535.1155453/94‑1 (MQ=60)
                AAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCA                                                                               >  SRR4302535.2828218/1‑94 (MQ=60)
                AAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCA                                                                               >  SRR4302535.1903988/1‑94 (MQ=60)
                AAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCA                                                                               <  SRR4302535.4601298/94‑1 (MQ=60)
                 AGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCAT                                                                              >  SRR4302535.2005593/1‑94 (MQ=60)
                  GCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATA                                                                             >  SRR4302535.620137/1‑94 (MQ=60)
                   CCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAA                                                                            >  SRR4302535.895991/1‑94 (MQ=60)
                   CCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAA                                                                            >  SRR4302535.724553/1‑94 (MQ=60)
                   CCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTGGCAAACAGTCCCATCATAA                                                                            >  SRR4302535.1209864/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           <  SRR4302535.2545082/94‑1 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           >  SRR4302535.309250/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           >  SRR4302535.4227864/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           >  SRR4302535.1864905/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           >  SRR4302535.938061/1‑94 (MQ=60)
                    CCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCACATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAAT                                                                           <  SRR4302535.3261556/94‑1 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAC                                                                         >  SRR4302535.3422471/1‑94 (MQ=60)
                      ATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATAC                                                                         >  SRR4302535.3466602/1‑94 (MQ=60)
                       TCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACT                                                                        <  SRR4302535.1082655/94‑1 (MQ=60)
                        CGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTG                                                                       <  SRR4302535.2438201/94‑1 (MQ=60)
                        CGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTG                                                                       >  SRR4302535.3395144/1‑94 (MQ=60)
                         GCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGC                                                                      >  SRR4302535.141880/1‑94 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCGGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     <  SRR4302535.3328916/94‑1 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     >  SRR4302535.2046828/1‑94 (MQ=60)
                          CCTGTACAACGACAAAAGCGAAGAAAGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCC                                                                     >  SRR4302535.1437503/1‑94 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCG                                                                    >  SRR4302535.1713648/1‑94 (MQ=60)
                           CTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCG                                                                    >  SRR4302535.446297/1‑94 (MQ=60)
                             GTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAA                                                                  >  SRR4302535.3284856/1‑94 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302535.327177/94‑1 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302535.412688/94‑1 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 <  SRR4302535.1041266/94‑1 (MQ=60)
                              TACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAAT                                                                 >  SRR4302535.1234032/1‑94 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                                <  SRR4302535.842582/94‑1 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                                >  SRR4302535.4128907/1‑94 (MQ=60)
                               ACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATG                                                                <  SRR4302535.2112908/94‑1 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                              <  SRR4302535.1039407/94‑1 (MQ=60)
                                 AACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCA                                                              >  SRR4302535.3193540/1‑94 (MQ=60)
                                  ACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGGGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAG                                                             >  SRR4302535.202673/1‑94 (MQ=60)
                                   CGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGTGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGC                                                            <  SRR4302535.2494415/94‑1 (MQ=60)
                                   CGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGC                                                            >  SRR4302535.3080811/1‑94 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           <  SRR4302535.4204752/94‑1 (MQ=60)
                                    GACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCG                                                           <  SRR4302535.2801177/94‑1 (MQ=60)
                                     ACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGA                                                          >  SRR4302535.3973534/1‑94 (MQ=60)
                                     ACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGA                                                          >  SRR4302535.3988507/1‑94 (MQ=60)
                                       AAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATC                                                        >  SRR4302535.2498441/1‑94 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                       >  SRR4302535.1997710/1‑94 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                       >  SRR4302535.833385/1‑94 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                       <  SRR4302535.1900885/94‑1 (MQ=60)
                                        AAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCA                                                       >  SRR4302535.1491903/1‑94 (MQ=60)
                                         AAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAG                                                      <  SRR4302535.1872061/94‑1 (MQ=60)
                                         AAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAG                                                      <  SRR4302535.12155/94‑1 (MQ=60)
                                          AGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGA                                                     >  SRR4302535.4530621/1‑94 (MQ=60)
                                          AGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGA                                                     <  SRR4302535.2993662/94‑1 (MQ=60)
                                           GCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAC                                                    <  SRR4302535.3816825/94‑1 (MQ=60)
                                            CGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACA                                                   >  SRR4302535.1839858/1‑94 (MQ=60)
                                              AAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGG                                                 <  SRR4302535.1118524/94‑1 (MQ=60)
                                               AGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGT                                                >  SRR4302535.2272807/1‑94 (MQ=60)
                                               AGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGT                                                >  SRR4302535.4581278/1‑94 (MQ=60)
                                               AGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGC                                                >  SRR4302535.1574575/1‑94 (MQ=60)
                                               AGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGA                                                >  SRR4302535.234494/1‑94 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            <  SRR4302535.1400467/94‑1 (MQ=60)
                                                   CGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTG                                            >  SRR4302535.2396582/1‑94 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           >  SRR4302535.276102/1‑94 (MQ=60)
                                                    GCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGA                                           >  SRR4302535.473637/1‑94 (MQ=60)
                                                     CATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGAC                                          <  SRR4302535.3765383/94‑1 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGNNGCGATCAGACAGGTNGNGNCG                                         >  SRR4302535.2002628/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302535.3737699/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302535.3981882/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         <  SRR4302535.259315/94‑1 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACG                                         >  SRR4302535.2767534/1‑94 (MQ=60)
                                                      ATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGCGACG                                         >  SRR4302535.3146497/1‑94 (MQ=60)
                                                       TTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGA                                        >  SRR4302535.2830921/1‑94 (MQ=60)
                                                       TTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGA                                        >  SRR4302535.3097797/1‑94 (MQ=60)
                                                         CAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAG                                      <  SRR4302535.2580592/94‑1 (MQ=60)
                                                          AGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGA                                     >  SRR4302535.594906/1‑94 (MQ=60)
                                                           GGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAC                                    <  SRR4302535.1483618/94‑1 (MQ=60)
                                                            GCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACG                                   <  SRR4302535.2231588/94‑1 (MQ=60)
                                                            GCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACG                                   <  SRR4302535.3530311/94‑1 (MQ=60)
                                                            GCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACG                                   <  SRR4302535.801574/94‑1 (MQ=60)
                                                             CCCATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGT                                  >  SRR4302535.4011751/1‑94 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCG                                >  SRR4302535.1888139/1‑94 (MQ=60)
                                                               CATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCG                                >  SRR4302535.1800519/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                               >  SRR4302535.2294352/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                               >  SRR4302535.685395/1‑94 (MQ=60)
                                                                ATAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGC                               >  SRR4302535.77046/1‑94 (MQ=60)
                                                                 TAGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCT                              <  SRR4302535.33396/94‑1 (MQ=60)
                                                                  AGCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTG                             >  SRR4302535.2154247/1‑94 (MQ=60)
                                                                  AGCGGGGGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATTCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTG                             <  SRR4302535.1065520/94‑1 (MQ=60)
                                                                   GCGGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGG                            >  SRR4302535.1089803/1‑94 (MQ=60)
                                                                     GGGTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTA                          >  SRR4302535.3679021/1‑94 (MQ=60)
                                                                       GTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATC                        >  SRR4302535.1649429/1‑94 (MQ=60)
                                                                       GTGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATC                        >  SRR4302535.2285830/1‑94 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                       >  SRR4302535.4129299/1‑94 (MQ=60)
                                                                        TGCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCC                       <  SRR4302535.4443865/94‑1 (MQ=60)
                                                                         GCCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCA                      >  SRR4302535.4195696/1‑94 (MQ=60)
                                                                          TCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302535.178617/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     >  SRR4302535.1825142/1‑94 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302535.3969300/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302535.3953470/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCAT                     <  SRR4302535.2240336/94‑1 (MQ=60)
                                                                          CCAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGGATCCAT                     >  SRR4302535.3605071/1‑94 (MQ=60)
                                                                           CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                    >  SRR4302535.1343471/1‑94 (MQ=60)
                                                                           CAGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATG                    >  SRR4302535.3995071/1‑94 (MQ=60)
                                                                            AGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGC                   >  SRR4302535.1760424/1‑94 (MQ=60)
                                                                            AGTGCAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGC                   <  SRR4302535.82743/94‑1 (MQ=60)
                                                                                CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               >  SRR4302535.4542931/1‑94 (MQ=60)
                                                                                CAACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGC               >  SRR4302535.2522775/1‑94 (MQ=60)
                                                                                 AACTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCA              <  SRR4302535.1369865/94‑1 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            >  SRR4302535.618447/1‑94 (MQ=60)
                                                                                   CTGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAAC            <  SRR4302535.1204654/94‑1 (MQ=60)
                                                                                    TGGCAGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACG           >  SRR4302535.183170/1‑94 (MQ=60)
                                                                                        AGGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAA       <  SRR4302535.2911119/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      <  SRR4302535.2631700/94‑1 (MQ=60)
                                                                                         GGTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAG      <  SRR4302535.1811119/94‑1 (MQ=60)
                                                                                          GTTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGA     >  SRR4302535.2025328/1‑94 (MQ=60)
                                                                                           TTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAA    <  SRR4302535.935454/94‑1 (MQ=60)
                                                                                           TTGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAA    <  SRR4302535.2261905/94‑1 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT   <  SRR4302535.4105866/94‑1 (MQ=60)
                                                                                            TGGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAAT   <  SRR4302535.47098/94‑1 (MQ=60)
                                                                                             GGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT  >  SRR4302535.2983609/1‑94 (MQ=60)
                                                                                             GGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT  <  SRR4302535.3406505/94‑1 (MQ=60)
                                                                                             GGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGTCGCTGGTATCCATGCCAGCAACGCCAAGAATT  >  SRR4302535.1965272/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATT  >  NC_000913/3893900‑3894086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑