Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,902,432 G→A P265L (CCG→CTG)  bglF ← PTS system beta‑glucosides‑specific enzyme IIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,902,4320GA100.0% 792.8 / NA 218P265L (CCG→CTG) bglFPTS system beta‑glucosides‑specific enzyme IIABC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (111/107);  total (111/107)

Only 100 of 226 total aligned reads displayed.

CCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCA  >  NC_000913/3902342‑3902521
                                                                                          |                                                                                         
  gccCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGccc                                                                                       >  2:2447289/1‑93 (MQ=255)
  ccccATTACCGCGCCCGCAAATGCAGGACCCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGccc                                                                                       <  2:624009/92‑1 (MQ=255)
    ccATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCcca                                                                                     >  1:4342441/1‑93 (MQ=255)
    ccATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCcca                                                                                     >  1:1758284/1‑93 (MQ=255)
       ttACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacca                                                                                  <  2:1678762/93‑1 (MQ=255)
       ttACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCccacca                                                                                  >  1:172138/1‑93 (MQ=255)
         aCCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACcagc                                                                                >  2:4436300/1‑93 (MQ=255)
            gcgcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGa                                                                             >  2:767562/1‑93 (MQ=255)
              gcCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGaaa                                                                           <  1:1592694/93‑1 (MQ=255)
                ccGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAgg                                                                         >  2:3622417/1‑93 (MQ=255)
                  gCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTg                                                                       >  1:1491517/1‑93 (MQ=255)
                   cAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGa                                                                      >  2:808826/1‑93 (MQ=255)
                   cAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGa                                                                      <  2:1936787/93‑1 (MQ=255)
                    aaaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGAc                                                                     <  1:1417417/93‑1 (MQ=255)
                    aaaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGAc                                                                     <  1:3072312/93‑1 (MQ=255)
                     aaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCCGCAGAAAGGTGACg                                                                    >  1:1854313/1‑93 (MQ=255)
                     aaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACg                                                                    <  1:4023567/93‑1 (MQ=255)
                     aaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACg                                                                    >  2:2275889/1‑93 (MQ=255)
                     aaTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACg                                                                    <  2:2726993/93‑1 (MQ=255)
                      aTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACgg                                                                   >  2:3966457/1‑93 (MQ=255)
                       tGCAGGAACGCCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACggg                                                                  <  1:1278176/93‑1 (MQ=255)
                       tGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACggg                                                                  <  2:2938670/93‑1 (MQ=255)
                        gCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgt                                                                 <  2:3782971/93‑1 (MQ=255)
                         cAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgtg                                                                <  1:4256190/93‑1 (MQ=255)
                          aGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGgtgt                                                               <  2:2384892/93‑1 (MQ=255)
                           ggAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGTTGACGGGtgtg                                                              >  2:2093040/1‑93 (MQ=255)
                            gacccGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGa                                                             <  2:1616601/90‑1 (MQ=255)
                              aCCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGCTAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATa                                                           <  1:658167/93‑1 (MQ=255)
                                 gCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAAcc                                                        >  2:4303146/1‑93 (MQ=255)
                                   cTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAt                                                      >  2:496050/1‑93 (MQ=255)
                                   cTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAt                                                      >  2:28609/1‑93 (MQ=255)
                                   cTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCAt                                                      <  1:2780066/93‑1 (MQ=255)
                                    tGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATc                                                     >  1:4093052/1‑93 (MQ=255)
                                     gATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCa                                                    <  1:3810725/93‑1 (MQ=255)
                                      aTAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAg                                                   <  2:369358/93‑1 (MQ=255)
                                      aTAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAg                                                   >  1:3910323/1‑93 (MQ=255)
                                        aaaGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGAc                                                 <  1:2641026/93‑1 (MQ=255)
                                         aaGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACa                                                <  1:1009419/93‑1 (MQ=255)
                                          aGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAt                                               <  1:3620270/93‑1 (MQ=255)
                                           gCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATa                                              >  2:1354495/1‑93 (MQ=255)
                                            ccAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAg                                             >  1:3253305/1‑93 (MQ=255)
                                             cAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGc                                            <  1:1333332/93‑1 (MQ=255)
                                              agagATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCa                                           <  2:4357244/93‑1 (MQ=255)
                                               gagaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCaa                                          >  1:2181719/1‑93 (MQ=255)
                                                agaTAACCGGCGTCAATCAGTACGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGCCGGGTGTGATAACCATCTGACTTAGCAAt                                         <  2:1065685/93‑1 (MQ=255)
                                                agaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAAt                                         <  2:523393/93‑1 (MQ=255)
                                                agaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAAt                                         <  2:4568730/93‑1 (MQ=255)
                                                 gaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATg                                        <  1:3855313/93‑1 (MQ=255)
                                                 gaTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATg                                        >  1:125858/1‑93 (MQ=255)
                                                  aTAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATgg                                       <  1:701756/93‑1 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      <  2:4063417/93‑1 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      >  2:2527568/1‑93 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      <  2:4123250/93‑1 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      <  1:4559675/93‑1 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      <  1:379650/93‑1 (MQ=255)
                                                   tAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgt                                      <  2:4187906/93‑1 (MQ=255)
                                                    aaCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgtg                                     <  1:4522159/93‑1 (MQ=255)
                                                    aaCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgtg                                     <  1:1541758/93‑1 (MQ=255)
                                                     aCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgtgt                                    <  1:3430934/93‑1 (MQ=255)
                                                     aCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGgtgt                                    <  2:3463273/93‑1 (MQ=255)
                                                        ggcggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaa                                 <  2:2679976/93‑1 (MQ=255)
                                                         gcggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaag                                <  1:1164669/93‑1 (MQ=255)
                                                         gcggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaag                                >  1:4278916/1‑93 (MQ=255)
                                                          cggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaaga                               <  1:2582305/93‑1 (MQ=255)
                                                           ggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaagaa                              >  1:2396870/1‑93 (MQ=255)
                                                           ggcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTgaagaa                              >  2:1116955/1‑93 (MQ=255)
                                                            gcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGaaa                             <  1:1654931/93‑1 (MQ=255)
                                                            gcAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGaaa                             >  1:525250/1‑93 (MQ=255)
                                                              aaTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGCAAGGTGACGGGAGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtt                           >  1:199297/1‑93 (MQ=255)
                                                              aaTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtt                           >  2:663141/1‑93 (MQ=255)
                                                              aaTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtt                           >  2:3143199/1‑93 (MQ=255)
                                                              aaTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtt                           >  1:803934/1‑93 (MQ=255)
                                                              aaTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtt                           >  2:1855076/1‑93 (MQ=255)
                                                              aaTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAAACATCAGACATAGCAATGGTGTGAAGAAAtt                           >  2:4106724/1‑93 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          <  1:1908433/93‑1 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          >  1:2833193/1‑93 (MQ=255)
                                                               aTCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttt                          <  1:953639/93‑1 (MQ=255)
                                                                tCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtttt                         <  2:944921/93‑1 (MQ=255)
                                                                tCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtttt                         <  1:3667035/93‑1 (MQ=255)
                                                                tCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAtttt                         <  1:720742/93‑1 (MQ=255)
                                                                 cAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAttttt                        <  1:1354495/93‑1 (MQ=255)
                                                                  aGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTg                       <  1:488923/93‑1 (MQ=255)
                                                                  aGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTg                       <  2:3289726/93‑1 (MQ=255)
                                                                   gTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGa                      >  2:1319659/1‑93 (MQ=255)
                                                                   gTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGa                      >  1:3045773/1‑93 (MQ=255)
                                                                     tCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGAtt                    >  2:1423586/1‑93 (MQ=255)
                                                                      cGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTg                   >  1:3157291/1‑93 (MQ=255)
                                                                       gCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGc                  <  1:2265541/93‑1 (MQ=255)
                                                                        cTTATCCAGGTTGATTGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGcc                 >  1:2972591/1‑93 (MQ=255)
                                                                        cTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTCATTGcc                 >  1:3526331/1‑93 (MQ=255)
                                                                        cTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGGGAAGAAATTTTTGATTGcg                 >  2:3911838/1‑92 (MQ=255)
                                                                         ttATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCg                >  2:3024846/1‑93 (MQ=255)
                                                                         ttATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCg                >  2:1732293/1‑93 (MQ=255)
                                                                            tCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACg             >  1:2535377/1‑93 (MQ=255)
                                                                                ggTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGCGTGAAGAAATTTTTGATTGCCGACGGTaa         >  2:2500328/1‑93 (MQ=255)
                                                                                 gTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAc        >  1:4043109/1‑93 (MQ=255)
                                                                                  ttGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAcc       >  1:917864/1‑93 (MQ=255)
                                                                                   tGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCa      <  2:1312073/93‑1 (MQ=255)
                                                                                    gATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCAc     <  2:3327785/93‑1 (MQ=255)
                                                                                     aTAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACg    <  2:305423/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCA  >  NC_000913/3902342‑3902521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  NC_000913/3902340‑3902525
                                                                                            |                                                                                             
AGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAG                                                                                              >  SRR4302535.656303/1‑94 (MQ=60)
  CCGCCCATTGCCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCC                                                                                            <  SRR4302535.3829629/94‑1 (MQ=60)
   CGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCC                                                                                           >  SRR4302535.3133222/1‑94 (MQ=60)
    GCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCC                                                                                          <  SRR4302535.939993/94‑1 (MQ=60)
     CCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCA                                                                                         >  SRR4302535.235572/1‑94 (MQ=60)
     CCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCA                                                                                         >  SRR4302535.1770994/1‑94 (MQ=60)
     CCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCA                                                                                         >  SRR4302535.1334281/1‑94 (MQ=60)
     CCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCA                                                                                         <  SRR4302535.4101434/94‑1 (MQ=60)
     CCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCA                                                                                         >  SRR4302535.4372138/1‑94 (MQ=60)
        ATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCA                                                                                      >  SRR4302535.4410571/1‑94 (MQ=60)
        ATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCA                                                                                      >  SRR4302535.1682808/1‑94 (MQ=60)
        ATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCA                                                                                      >  SRR4302535.173332/1‑94 (MQ=60)
        ATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCA                                                                                      >  SRR4302535.784745/1‑94 (MQ=60)
           ACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCA                                                                                   >  SRR4302535.2750555/1‑94 (MQ=60)
              GCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAGCCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAA                                                                                <  SRR4302535.3132164/94‑1 (MQ=60)
               CGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAA                                                                               >  SRR4302535.2727697/1‑94 (MQ=60)
                GCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAG                                                                              <  SRR4302535.1604189/94‑1 (MQ=60)
                GCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAG                                                                              <  SRR4302535.579089/94‑1 (MQ=60)
                  CCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGT                                                                            >  SRR4302535.1535795/1‑94 (MQ=60)
                   CGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTG                                                                           >  SRR4302535.1502229/1‑94 (MQ=60)
                   CGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTG                                                                           >  SRR4302535.3238630/1‑94 (MQ=60)
                      AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCCGCAGAAAGGTGACG                                                                        >  SRR4302535.1867670/1‑94 (MQ=60)
                      AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        <  SRR4302535.3093762/94‑1 (MQ=60)
                      AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        >  SRR4302535.1634380/1‑94 (MQ=60)
                      AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        <  SRR4302535.1427538/94‑1 (MQ=60)
                      AAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACG                                                                        <  SRR4302535.1236795/94‑1 (MQ=60)
                       AATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGG                                                                       >  SRR4302535.2578991/1‑94 (MQ=60)
                       AATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGG                                                                       <  SRR4302535.4051096/94‑1 (MQ=60)
                        ATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGG                                                                      <  SRR4302535.3491047/94‑1 (MQ=60)
                        ATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGG                                                                      <  SRR4302535.672676/94‑1 (MQ=60)
                        ATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACAAGCAGAAAGGTGACGGG                                                                      >  SRR4302535.731592/1‑94 (MQ=60)
                         TGCAGGAACGCCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGT                                                                     <  SRR4302535.1287305/94‑1 (MQ=60)
                         TGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGT                                                                     <  SRR4302535.1733938/94‑1 (MQ=60)
                          GCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTG                                                                    <  SRR4302535.2251566/94‑1 (MQ=60)
                           CAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCGGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGT                                                                   >  SRR4302535.2988859/1‑94 (MQ=60)
                           CAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGT                                                                   >  SRR4302535.523890/1‑94 (MQ=60)
                           CAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGT                                                                   <  SRR4302535.4285347/94‑1 (MQ=60)
                             GGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGA                                                                 <  SRR4302535.1859840/94‑1 (MQ=60)
                               AACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATA                                                               >  SRR4302535.3577704/1‑94 (MQ=60)
                                ACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGCTAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAA                                                              <  SRR4302535.662762/94‑1 (MQ=60)
                                   GCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCA                                                           <  SRR4302535.1351799/94‑1 (MQ=60)
                                   GCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCA                                                           >  SRR4302535.1456403/1‑94 (MQ=60)
                                     CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         <  SRR4302535.2799860/94‑1 (MQ=60)
                                     CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         <  SRR4302535.531109/94‑1 (MQ=60)
                                     CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302535.3086990/1‑94 (MQ=60)
                                     CTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATC                                                         >  SRR4302535.4121061/1‑94 (MQ=60)
                                       GATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAG                                                       >  SRR4302535.1916249/1‑94 (MQ=60)
                                       GATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAG                                                       <  SRR4302535.3837018/94‑1 (MQ=60)
                                       GATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAG                                                       >  SRR4302535.3937099/1‑94 (MQ=60)
                                         TAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGAC                                                     <  SRR4302535.714256/94‑1 (MQ=60)
                                          AAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACA                                                    <  SRR4302535.2659945/94‑1 (MQ=60)
                                           AAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAT                                                   <  SRR4302535.438192/94‑1 (MQ=60)
                                           AAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACAT                                                   <  SRR4302535.1016591/94‑1 (MQ=60)
                                            AGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATA                                                  >  SRR4302535.4051994/1‑94 (MQ=60)
                                            AGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATA                                                  <  SRR4302535.3645366/94‑1 (MQ=60)
                                             GCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAG                                                 >  SRR4302535.2840533/1‑94 (MQ=60)
                                             GCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAG                                                 >  SRR4302535.3275908/1‑94 (MQ=60)
                                              CCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGC                                                <  SRR4302535.3568186/94‑1 (MQ=60)
                                               CAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCA                                               <  SRR4302535.1342841/94‑1 (MQ=60)
                                               CAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCA                                               >  SRR4302535.3001206/1‑94 (MQ=60)
                                                AGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAA                                              >  SRR4302535.2197280/1‑94 (MQ=60)
                                                  AGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATG                                            >  SRR4302535.126745/1‑94 (MQ=60)
                                                   GATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGG                                           <  SRR4302535.1750492/94‑1 (MQ=60)
                                                   GATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGG                                           <  SRR4302535.3881861/94‑1 (MQ=60)
                                                    ATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGT                                          <  SRR4302535.706677/94‑1 (MQ=60)
                                                     TAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTG                                         <  SRR4302535.4590801/94‑1 (MQ=60)
                                                     TAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTG                                         <  SRR4302535.382278/94‑1 (MQ=60)
                                                      AACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGT                                        <  SRR4302535.1552872/94‑1 (MQ=60)
                                                      AACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGT                                        <  SRR4302535.4553147/94‑1 (MQ=60)
                                                       ACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTG                                       <  SRR4302535.3454779/94‑1 (MQ=60)
                                                        CCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGA                                      <  SRR4302535.4042068/94‑1 (MQ=60)
                                                          GGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAG                                    >  SRR4302535.4308197/1‑94 (MQ=60)
                                                           GCGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGA                                   <  SRR4302535.1172987/94‑1 (MQ=60)
                                                            CGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAA                                  >  SRR4302535.2414095/1‑94 (MQ=60)
                                                            CGGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAA                                  <  SRR4302535.2600857/94‑1 (MQ=60)
                                                             GGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAA                                 >  SRR4302535.1961968/1‑94 (MQ=60)
                                                             GGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAA                                 >  SRR4302535.528918/1‑94 (MQ=60)
                                                             GGCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAA                                 >  SRR4302535.3885787/1‑94 (MQ=60)
                                                              GCAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAAT                                <  SRR4302535.1666902/94‑1 (MQ=60)
                                                               CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGCAAGGTGACGGGAGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               >  SRR4302535.200662/1‑94 (MQ=60)
                                                               CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               >  SRR4302535.877874/1‑94 (MQ=60)
                                                               CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               >  SRR4302535.809579/1‑94 (MQ=60)
                                                               CAATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATT                               <  SRR4302535.2131876/94‑1 (MQ=60)
                                                                AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              <  SRR4302535.415742/94‑1 (MQ=60)
                                                                AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302535.4332290/1‑94 (MQ=60)
                                                                AATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTT                              >  SRR4302535.2853304/1‑94 (MQ=60)
                                                                 ATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTT                             <  SRR4302535.960395/94‑1 (MQ=60)
                                                                 ATCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTT                             <  SRR4302535.1922191/94‑1 (MQ=60)
                                                                  TCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTT                            <  SRR4302535.725804/94‑1 (MQ=60)
                                                                  TCAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTT                            <  SRR4302535.3692428/94‑1 (MQ=60)
                                                                   CAGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTG                           <  SRR4302535.1364183/94‑1 (MQ=60)
                                                                    AGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGA                          <  SRR4302535.492349/94‑1 (MQ=60)
                                                                    AGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGA                          >  SRR4302535.4675664/1‑94 (MQ=60)
                                                                    AGTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGA                          >  SRR4302535.3067107/1‑94 (MQ=60)
                                                                     GTTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGAT                         >  SRR4302535.3414994/1‑94 (MQ=60)
                                                                      TTCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATT                        <  SRR4302535.2963853/94‑1 (MQ=60)
                                                                       TCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTG                       >  SRR4302535.3179210/1‑94 (MQ=60)
                                                                       TCGCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGCGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTG                       >  SRR4302535.3648863/1‑94 (MQ=60)
                                                                        CGCTTATCCAGGTTGACAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGC                      <  SRR4302535.2420097/94‑1 (MQ=60)
                                                                         GCTTATCCAGGTTGATTGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCC                     >  SRR4302535.2993455/1‑94 (MQ=60)
                                                                         GCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCC                     >  SRR4302535.1031995/1‑94 (MQ=60)
                                                                         GCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCC                     <  SRR4302535.2281685/94‑1 (MQ=60)
                                                                         GCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCC                     >  SRR4302535.2657365/1‑94 (MQ=60)
                                                                         GCTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTCATTGCC                     >  SRR4302535.3550818/1‑94 (MQ=60)
                                                                          CTTATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCG                    >  SRR4302535.157434/1‑94 (MQ=60)
                                                                            TATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGAC                  <  SRR4302535.1413941/94‑1 (MQ=60)
                                                                             ATCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACG                 >  SRR4302535.2553640/1‑94 (MQ=60)
                                                                              TCCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGG                <  SRR4302535.4571072/94‑1 (MQ=60)
                                                                               CCAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGT               >  SRR4302535.1338096/1‑94 (MQ=60)
                                                                                CAGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTA              <  SRR4302535.1533232/94‑1 (MQ=60)
                                                                                 AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             >  SRR4302535.4289476/1‑94 (MQ=60)
                                                                                 AGGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAA             >  SRR4302535.3773391/1‑94 (MQ=60)
                                                                                  GGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAC            >  SRR4302535.4070786/1‑94 (MQ=60)
                                                                                  GGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAC            <  SRR4302535.2043650/94‑1 (MQ=60)
                                                                                  GGTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAAC            >  SRR4302535.2589996/1‑94 (MQ=60)
                                                                                   GTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACC           >  SRR4302535.1275380/1‑94 (MQ=60)
                                                                                   GTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACC           >  SRR4302535.924374/1‑94 (MQ=60)
                                                                                   GTTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACC           <  SRR4302535.3860379/94‑1 (MQ=60)
                                                                                    TTGATAGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCA          >  SRR4302535.3842653/1‑94 (MQ=60)
                                                                                         AGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCAT     >  SRR4302535.3224040/1‑94 (MQ=60)
                                                                                         AGCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCAT     <  SRR4302535.4081744/94‑1 (MQ=60)
                                                                                          GCAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATT    <  SRR4302535.36763/94‑1 (MQ=60)
                                                                                           CAGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTA   >  SRR4302535.1093708/1‑94 (MQ=60)
                                                                                            AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  SRR4302535.2541560/1‑94 (MQ=60)
                                                                                            AGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  SRR4302535.3501486/1‑94 (MQ=60)
                                                                                            |                                                                                             
AGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAA  >  NC_000913/3902340‑3902525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑