Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,905,748 A→G S83P (TCT→CCT)  pstB ← ATP‑binding component of high‑affinity phosphate‑specific transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,905,7480AG100.0% 705.0 / NA 224S83P (TCT→CCT) pstBATP‑binding component of high‑affinity phosphate‑specific transport system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (109/115);  total (109/115)

Only 100 of 237 total aligned reads displayed.

AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA  >  NC_000913/3905658‑3905837
                                                                                          |                                                                                         
aaGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCATCAGTGCGATATCCTGAGGGt                                                                                         >  1:3127191/1‑93 (MQ=255)
aaGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGt                                                                                         >  2:1487773/1‑93 (MQ=255)
 aGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGtt                                                                                        <  2:1507290/93‑1 (MQ=255)
 aGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGtt                                                                                        <  2:1956787/93‑1 (MQ=255)
  gCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTg                                                                                       <  2:3663220/93‑1 (MQ=255)
  gCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTg                                                                                       <  1:3689613/93‑1 (MQ=255)
    gATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGt                                                                                     >  2:2508732/1‑93 (MQ=255)
    gATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGt                                                                                     >  2:511128/1‑93 (MQ=255)
    gATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGt                                                                                     <  1:1067102/93‑1 (MQ=255)
      tgttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCa                                                                                   <  2:372510/93‑1 (MQ=255)
      tgttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCa                                                                                   <  2:2027912/93‑1 (MQ=255)
      tgttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCa                                                                                   >  1:4090151/1‑93 (MQ=255)
        ttgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAgg                                                                                 >  1:2888714/1‑93 (MQ=255)
         tgtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGa                                                                                <  1:1642910/93‑1 (MQ=255)
          gtCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAt                                                                               <  1:1985314/93‑1 (MQ=255)
           tCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtg                                                                              <  1:3806063/93‑1 (MQ=255)
           tCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtg                                                                              >  1:2615582/1‑93 (MQ=255)
             gTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgtt                                                                            <  2:2651348/93‑1 (MQ=255)
              tAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgttg                                                                           >  1:2004859/1‑93 (MQ=255)
              tAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgttg                                                                           <  2:1693750/93‑1 (MQ=255)
              tAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgttg                                                                           >  1:3905719/1‑93 (MQ=255)
              tAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgttg                                                                           <  1:1300331/93‑1 (MQ=255)
              tAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAtgttg                                                                           >  2:929147/1‑93 (MQ=255)
                gATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTc                                                                         >  1:27989/1‑93 (MQ=255)
                  tGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGc                                                                       >  1:1348543/1‑93 (MQ=255)
                   ggACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGcc                                                                      >  2:2623653/1‑93 (MQ=255)
                    gACATCGGAAGCGGCGTCGGTGTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCa                                                                     <  1:1231382/93‑1 (MQ=255)
                    gACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCa                                                                     <  2:2175823/93‑1 (MQ=255)
                    gACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCa                                                                     >  2:3955298/1‑93 (MQ=255)
                        tCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCaa                                                                 <  1:267050/93‑1 (MQ=255)
                        tCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCaa                                                                 >  2:1095921/1‑93 (MQ=255)
                        tCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCaa                                                                 <  1:2306210/93‑1 (MQ=255)
                          ggAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGc                                                               <  2:3133838/93‑1 (MQ=255)
                          ggAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGc                                                               >  1:4603624/1‑93 (MQ=255)
                          ggAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGc                                                               <  2:1942275/93‑1 (MQ=255)
                           gAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCa                                                              >  2:1014500/1‑93 (MQ=255)
                           gAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCa                                                              >  1:3675519/1‑93 (MQ=255)
                            aaaCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAg                                                             >  1:3795542/1‑93 (MQ=255)
                             aaCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                            >  2:2417490/1‑93 (MQ=255)
                             aaCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                            >  1:1820669/1‑93 (MQ=255)
                             aaCGGCGGCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCACTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                            >  2:3072064/1‑93 (MQ=255)
                              aCGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGaa                                                           <  2:1538709/93‑1 (MQ=255)
                               cGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAt                                                          >  2:3907044/1‑93 (MQ=255)
                                ggCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                                         <  1:2429702/93‑1 (MQ=255)
                                 gCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAttt                                                        >  2:740228/1‑93 (MQ=255)
                                  cGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTc                                                       >  1:3722424/1‑93 (MQ=255)
                                   gTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCa                                                      >  2:4159786/1‑93 (MQ=255)
                                   gTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCa                                                      >  1:3182349/1‑93 (MQ=255)
                                    gcGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAc                                                     <  1:1898838/92‑1 (MQ=255)
                                     cGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAcc                                                    <  2:3249445/93‑1 (MQ=255)
                                      ggTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCt                                                   >  2:1421659/1‑93 (MQ=255)
                                        tttCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                                 >  2:4303916/1‑93 (MQ=255)
                                          tCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCg                                               <  2:1463014/93‑1 (MQ=255)
                                          tCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCg                                               <  2:4052975/93‑1 (MQ=255)
                                             ggAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAc                                            <  1:4433865/93‑1 (MQ=255)
                                                acacCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgct                                         >  1:3089854/1‑93 (MQ=255)
                                                    cATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                                     >  2:4663207/1‑93 (MQ=255)
                                                    cATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                                     <  1:1597826/93‑1 (MQ=255)
                                                     aTGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTcc                                    <  2:1654095/93‑1 (MQ=255)
                                                     aTGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTcc                                    <  2:582340/93‑1 (MQ=255)
                                                     aTGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTcc                                    >  2:3675812/1‑93 (MQ=255)
                                                       gCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCgg                                  <  2:347695/93‑1 (MQ=255)
                                                         ccACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGt                                <  1:1977927/93‑1 (MQ=255)
                                                         ccACTTGCGCACGCAGCAGTGCGATGTCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCGCGCTGCTCCGGGt                                <  2:3186140/93‑1 (MQ=255)
                                                          cACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                               >  1:3257166/1‑93 (MQ=255)
                                                          cACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGATa                               >  2:4499252/1‑93 (MQ=255)
                                                           aCTTTAGCACGCAGCAGTGCGATATCCTGAGGGTTGGCCAGGATGTTGTCGCCATAAAGCAGAATATCATCTTCCGCACGCTGATCCggggcc                              >  2:138990/1‑90 (MQ=255)
                                                            cTTTCGCACGCATCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                             <  1:4285479/93‑1 (MQ=255)
                                                             tttCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg                            >  2:3422679/1‑93 (MQ=255)
                                                              ttCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGt                           <  1:819987/93‑1 (MQ=255)
                                                                cgcacgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTc                         >  1:3407856/1‑93 (MQ=255)
                                                                 gcacgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCa                        <  2:4084055/93‑1 (MQ=255)
                                                                    cgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAc                     <  1:3347242/93‑1 (MQ=255)
                                                                    cgcaGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAc                     <  1:4352278/93‑1 (MQ=255)
                                                                     gcagcaGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACa                    >  1:539971/1‑93 (MQ=255)
                                                                      cagcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAt                   >  1:4568154/1‑93 (MQ=255)
                                                                      cagcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAt                   >  2:3553285/1‑93 (MQ=255)
                                                                       agcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAtt                  >  2:2953852/1‑93 (MQ=255)
                                                                        gcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt                 >  1:959518/1‑93 (MQ=255)
                                                                        gcagTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAttt                 >  1:4389487/1‑93 (MQ=255)
                                                                          agTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttg               >  2:4425574/1‑93 (MQ=255)
                                                                           gTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgt              >  1:4482628/1‑93 (MQ=255)
                                                                            tGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgtt             <  1:1205654/93‑1 (MQ=255)
                                                                             gCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgttg            <  1:2113085/93‑1 (MQ=255)
                                                                              cGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGa           <  1:2910309/93‑1 (MQ=255)
                                                                               gATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGaa          <  2:853914/93‑1 (MQ=255)
                                                                               gATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTCaa          >  2:2264862/1‑93 (MQ=255)
                                                                               gATATCCTGAGGGTTGGTCAAGATGTTGTCGCCCTCAAGCAGAATTTTACCTTTCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGaa          <  1:2387588/93‑1 (MQ=255)
                                                                                atatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAg         <  2:1565529/93‑1 (MQ=255)
                                                                                atatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAg         >  2:2559985/1‑93 (MQ=255)
                                                                                atatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAg         <  2:2822261/93‑1 (MQ=255)
                                                                                 tatCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAgg        >  2:1608374/1‑93 (MQ=255)
                                                                                  atCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGt       >  2:707800/1‑93 (MQ=255)
                                                                                   tCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTa      >  1:4200251/1‑93 (MQ=255)
                                                                                    ccTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTAc     <  1:3052013/93‑1 (MQ=255)
                                                                                    ccTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTAc     <  2:2309021/93‑1 (MQ=255)
                                                                                     cTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACg    <  1:3095590/93‑1 (MQ=255)
                                                                                      tGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACgc   >  2:4123852/1‑93 (MQ=255)
                                                                                       gaggGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACgca  <  1:1490602/93‑1 (MQ=255)
                                                                                       gaggGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACgca  <  2:3311451/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA  >  NC_000913/3905658‑3905837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  >  NC_000913/3905655‑3905841
                                                                                             |                                                                                             
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               >  SRR4302535.930867/1‑94 (MQ=60)
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               <  SRR4302535.1260436/94‑1 (MQ=60)
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGG                                                                                               <  SRR4302535.2999381/94‑1 (MQ=60)
 AAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGG                                                                                              >  SRR4302535.1800866/1‑94 (MQ=60)
 AAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGG                                                                                              <  SRR4302535.3257817/94‑1 (MQ=60)
 AAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGG                                                                                              <  SRR4302535.4341608/94‑1 (MQ=60)
  AAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCATCAGTGCGATATCCTGAGGGT                                                                                             >  SRR4302535.3148970/1‑94 (MQ=60)
    AGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTG                                                                                           <  SRR4302535.1939619/94‑1 (MQ=60)
     GCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGG                                                                                          <  SRR4302535.3715152/94‑1 (MQ=60)
     GCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGG                                                                                          <  SRR4302535.3087277/94‑1 (MQ=60)
     GCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGG                                                                                          <  SRR4302535.2682948/94‑1 (MQ=60)
       GATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTC                                                                                        <  SRR4302535.1074690/94‑1 (MQ=60)
        ATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCA                                                                                       >  SRR4302535.1379702/1‑94 (MQ=60)
        ATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCA                                                                                       >  SRR4302535.4118135/1‑94 (MQ=60)
        ATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCA                                                                                       >  SRR4302535.2218418/1‑94 (MQ=60)
        ATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCA                                                                                       >  SRR4302535.1993339/1‑94 (MQ=60)
          GTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGG                                                                                     <  SRR4302535.4634699/94‑1 (MQ=60)
          GTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGG                                                                                     >  SRR4302535.2909151/1‑94 (MQ=60)
            TGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAT                                                                                   <  SRR4302535.1623028/94‑1 (MQ=60)
            TGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGAT                                                                                   <  SRR4302535.1654786/94‑1 (MQ=60)
             GTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATG                                                                                  <  SRR4302535.1999608/94‑1 (MQ=60)
             GTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATG                                                                                  >  SRR4302535.2634338/1‑94 (MQ=60)
             GTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATG                                                                                  <  SRR4302535.1239506/94‑1 (MQ=60)
             GTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATG                                                                                  <  SRR4302535.3973406/94‑1 (MQ=60)
              TCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGT                                                                                 <  SRR4302535.3832302/94‑1 (MQ=60)
                GTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTG                                                                               <  SRR4302535.3111665/94‑1 (MQ=60)
                GTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTG                                                                               >  SRR4302535.3205485/1‑94 (MQ=60)
                GTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTG                                                                               >  SRR4302535.2019302/1‑94 (MQ=60)
                GTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTG                                                                               >  SRR4302535.3932479/1‑94 (MQ=60)
                 TAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGT                                                                              <  SRR4302535.1309600/94‑1 (MQ=60)
                  AGATGGACATCGGAAACGGCGTGGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTC                                                                             <  SRR4302535.4147977/94‑1 (MQ=60)
                  AGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTC                                                                             >  SRR4302535.28210/1‑94 (MQ=60)
                    ATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGC                                                                           >  SRR4302535.4291683/1‑94 (MQ=60)
                    ATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGC                                                                           >  SRR4302535.1358174/1‑94 (MQ=60)
                    ATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGC                                                                           >  SRR4302535.152138/1‑94 (MQ=60)
                       GACATCGGAAGCGGCGTCGGTGTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCAT                                                                        <  SRR4302535.1240187/94‑1 (MQ=60)
                         CATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCA                                                                      >  SRR4302535.4502621/1‑94 (MQ=60)
                          ATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAC                                                                     >  SRR4302535.3979293/1‑94 (MQ=60)
                           TCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAG                                                                    <  SRR4302535.2322705/94‑1 (MQ=60)
                           TCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAG                                                                    <  SRR4302535.1995305/94‑1 (MQ=60)
                           TCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAG                                                                    <  SRR4302535.268904/94‑1 (MQ=60)
                            CGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGC                                                                   >  SRR4302535.4634924/1‑94 (MQ=60)
                             GGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCA                                                                  >  SRR4302535.3737297/1‑94 (MQ=60)
                             GGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCA                                                                  >  SRR4302535.3700968/1‑94 (MQ=60)
                              GAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAG                                                                 >  SRR4302535.3821706/1‑94 (MQ=60)
                               AAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGA                                                                >  SRR4302535.1833823/1‑94 (MQ=60)
                                AACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAA                                                               >  SRR4302535.2960370/1‑94 (MQ=60)
                                  CGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATT                                                             >  SRR4302535.1346816/1‑94 (MQ=60)
                                  CGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATT                                                             >  SRR4302535.2367625/1‑94 (MQ=60)
                                   GGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTT                                                            >  SRR4302535.1279181/1‑94 (MQ=60)
                                   GGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTT                                                            <  SRR4302535.2447207/94‑1 (MQ=60)
                                   GGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTT                                                            <  SRR4302535.2746608/94‑1 (MQ=60)
                                   GGCGTCGGTTTCTGCAGCACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTT                                                            <  SRR4302535.2746607/94‑1 (MQ=60)
                                    GCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTC                                                           >  SRR4302535.1540081/1‑94 (MQ=60)
                                    GCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTC                                                           >  SRR4302535.3748211/1‑94 (MQ=60)
                                     CGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCA                                                          >  SRR4302535.129571/1‑94 (MQ=60)
                                     CGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCA                                                          >  SRR4302535.3204340/1‑94 (MQ=60)
                                      GTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAC                                                         <  SRR4302535.3475245/94‑1 (MQ=60)
                                       GCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACC                                                        <  SRR4302535.1912503/94‑1 (MQ=60)
                                          GTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTC                                                     >  SRR4302535.892935/1‑94 (MQ=60)
                                           TTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCC                                                    <  SRR4302535.2922836/94‑1 (MQ=60)
                                                GGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACG                                               >  SRR4302535.731132/1‑94 (MQ=60)
                                                GGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACG                                               <  SRR4302535.4464246/94‑1 (MQ=60)
                                                GGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACG                                               <  SRR4302535.4708430/94‑1 (MQ=60)
                                                  AACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCT                                             >  SRR4302535.3111374/1‑94 (MQ=60)
                                                  AACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCT                                             >  SRR4302535.4444583/1‑94 (MQ=60)
                                                    CACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGATGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGC                                           >  SRR4302535.3322020/1‑94 (MQ=60)
                                                      CCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTC                                         >  SRR4302535.2098449/1‑94 (MQ=60)
                                                      CCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTC                                         >  SRR4302535.3632249/1‑94 (MQ=60)
                                                       CATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCC                                        <  SRR4302535.1609350/94‑1 (MQ=60)
                                                       CATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCC                                        >  SRR4302535.4283771/1‑94 (MQ=60)
                                                         TGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGG                                      <  SRR4302535.3823662/94‑1 (MQ=60)
                                                           CCCACTTTCGCACGCAGCAGTGCTATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGT                                    <  SRR4302535.4603733/94‑1 (MQ=60)
                                                           CCCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGT                                    >  SRR4302535.4422913/1‑94 (MQ=60)
                                                            CCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTA                                   <  SRR4302535.1992173/94‑1 (MQ=60)
                                                            CCACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTA                                   >  SRR4302535.3279797/1‑94 (MQ=60)
                                                             CACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTAC                                  >  SRR4302535.242914/1‑94 (MQ=60)
                                                              ACTTTCGCACGCAGCAGTGCGATATCCTGAGGGTTTGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACA                                 <  SRR4302535.3139567/94‑1 (MQ=60)
                                                               CTTTCGCACGCATCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAG                                <  SRR4302535.4314796/94‑1 (MQ=60)
                                                                TTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGT                               <  SRR4302535.2556617/94‑1 (MQ=60)
                                                                TTTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGT                               <  SRR4302535.4361495/94‑1 (MQ=60)
                                                                 TTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTT                              <  SRR4302535.825742/94‑1 (MQ=60)
                                                                 TTCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTT                              >  SRR4302535.1678870/1‑94 (MQ=60)
                                                                  TCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTC                             <  SRR4302535.624233/94‑1 (MQ=60)
                                                                  TCGCACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTC                             >  SRR4302535.3431578/1‑94 (MQ=60)
                                                                      ACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAC                         <  SRR4302535.2899128/94‑1 (MQ=60)
                                                                      ACGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGAAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAAC                         >  SRR4302535.298732/1‑94 (MQ=60)
                                                                       CGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACA                        <  SRR4302535.4382061/94‑1 (MQ=60)
                                                                       CGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACA                        >  SRR4302535.989062/1‑94 (MQ=60)
                                                                       CGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACA                        >  SRR4302535.543739/1‑94 (MQ=60)
                                                                       CGCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACA                        <  SRR4302535.3370560/94‑1 (MQ=60)
                                                                        GCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAT                       <  SRR4302535.3126939/94‑1 (MQ=60)
                                                                        GCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAT                       <  SRR4302535.2479239/94‑1 (MQ=60)
                                                                        GCAGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACAT                       >  SRR4302535.4599310/1‑94 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     >  SRR4302535.1856872/1‑94 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     >  SRR4302535.966331/1‑94 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     <  SRR4302535.4388484/94‑1 (MQ=60)
                                                                          AGCAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTT                     >  SRR4302535.4419520/1‑94 (MQ=60)
                                                                            CAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG                   <  SRR4302535.3329530/94‑1 (MQ=60)
                                                                            CAGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTG                   <  SRR4302535.4586205/94‑1 (MQ=60)
                                                                             AGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGT                  >  SRR4302535.4513393/1‑94 (MQ=60)
                                                                             AGTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGT                  >  SRR4302535.2222690/1‑94 (MQ=60)
                                                                              GTGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTT                 <  SRR4302535.3170725/94‑1 (MQ=60)
                                                                               TGCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTG                <  SRR4302535.1214314/94‑1 (MQ=60)
                                                                                GCGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGA               <  SRR4302535.2128335/94‑1 (MQ=60)
                                                                                 CGATATCCTGAGGGTTTGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              <  SRR4302535.3785224/94‑1 (MQ=60)
                                                                                 CGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              <  SRR4302535.2930869/94‑1 (MQ=60)
                                                                                 CGATATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA              <  SRR4302535.3785223/94‑1 (MQ=60)
                                                                                  GATATCCTGAGGGTTGGTCAAGATGTTGTCGCCCTCAAGCAGAATTTTACCTTTCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAG             <  SRR4302535.2404753/94‑1 (MQ=60)
                                                                                     ATCCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTA          >  SRR4302535.4228994/1‑94 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        <  SRR4302535.2011167/94‑1 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        <  SRR4302535.3073389/94‑1 (MQ=60)
                                                                                       CCTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACG        <  SRR4302535.3707201/94‑1 (MQ=60)
                                                                                        CTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGC       <  SRR4302535.3117148/94‑1 (MQ=60)
                                                                                        CTGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGC       >  SRR4302535.3890631/1‑94 (MQ=60)
                                                                                         TGAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCA      >  SRR4302535.1084535/1‑94 (MQ=60)
                                                                                          GAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAG     <  SRR4302535.3381095/94‑1 (MQ=60)
                                                                                          GAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAG     <  SRR4302535.18496/94‑1 (MQ=60)
                                                                                          GAGGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAG     <  SRR4302535.1501305/94‑1 (MQ=60)
                                                                                            GGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCA   >  SRR4302535.3711097/1‑94 (MQ=60)
                                                                                            GGGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCA   <  SRR4302535.1924019/94‑1 (MQ=60)
                                                                                             GGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  <  SRR4302535.2096810/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAAGGTACGCAGCAG  >  NC_000913/3905655‑3905841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑