Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,911,249 G→A I16I (ATC→ATT)  glmS ← L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,911,2490GA99.6% 926.0 / NA 255I16I (ATC→ATT) glmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (109/145);  minor base C (1/0);  total (110/145)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

CAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTAT  >  NC_000913/3911159‑3911338
                                                                                          |                                                                                         
cAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAAt                                                                                         <  2:2990783/93‑1 (MQ=255)
cAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAAt                                                                                         <  2:4534111/93‑1 (MQ=255)
cAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAAt                                                                                         <  1:495924/93‑1 (MQ=255)
 aGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAAtt                                                                                        <  1:232471/93‑1 (MQ=255)
 aGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAAtt                                                                                        <  1:1427742/93‑1 (MQ=255)
  ggCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAAttt                                                                                       >  1:238916/1‑93 (MQ=255)
    cGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCt                                                                                     <  2:413477/93‑1 (MQ=255)
    cGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCt                                                                                     <  1:2170323/93‑1 (MQ=255)
     gggTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTg                                                                                    >  2:730474/1‑93 (MQ=255)
      ggTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGc                                                                                   <  2:3615006/93‑1 (MQ=255)
      ggTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGc                                                                                   <  1:1065006/93‑1 (MQ=255)
      ggTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGc                                                                                   >  1:3266612/1‑93 (MQ=255)
         cATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTAc                                                                                >  2:4406929/1‑93 (MQ=255)
          atatGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACa                                                                               >  1:1595865/1‑93 (MQ=255)
          atatGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACa                                                                               <  2:2050745/93‑1 (MQ=255)
           tatGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACAt                                                                              <  1:1681348/93‑1 (MQ=255)
            atGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATc                                                                             >  2:3158654/1‑93 (MQ=255)
            atGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATc                                                                             <  2:1048286/93‑1 (MQ=255)
            atGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATc                                                                             >  1:865987/1‑93 (MQ=255)
             tGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCa                                                                            >  2:2693254/1‑93 (MQ=255)
              gACCTTCTGCATCAACAACGCCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCAc                                                                           >  2:4514965/1‑93 (MQ=255)
                ccTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGt                                                                         >  1:4126346/1‑93 (MQ=255)
                ccTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGt                                                                         <  2:1608156/93‑1 (MQ=255)
                 cTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGtt                                                                        <  1:2522845/93‑1 (MQ=255)
                 cTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGtt                                                                        >  2:886236/1‑93 (MQ=255)
                     tGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTgcgc                                                                    <  2:3419444/93‑1 (MQ=255)
                     tGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTgcgc                                                                    <  2:2536688/93‑1 (MQ=255)
                      gCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGcgcg                                                                   >  1:2590136/1‑93 (MQ=255)
                       cATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGa                                                                  <  1:4459817/93‑1 (MQ=255)
                       cATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGa                                                                  <  1:1097304/93‑1 (MQ=255)
                        aTNAACNACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGAt                                                                 <  1:1271594/93‑1 (MQ=255)
                        aTCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGAt                                                                 >  2:1385354/1‑93 (MQ=255)
                         tCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATc                                                                <  1:471541/93‑1 (MQ=255)
                         tCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATc                                                                <  2:1800273/93‑1 (MQ=255)
                         tCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATc                                                                >  2:4022367/1‑93 (MQ=255)
                           aacaacGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATcgc                                                              >  1:345958/1‑93 (MQ=255)
                            acaacGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATcgcg                                                             >  2:538446/1‑93 (MQ=255)
                            acaacGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATcgcg                                                             <  2:978172/93‑1 (MQ=255)
                            acaacGGCCAGACCGGCAGAGGAACAGCCCCGGGAGTCCAGACGGCGGAAACCTCCAAGAAGAATTTCGGGTACATCACGCTGCGGGATcgcg                                                             >  2:335991/1‑93 (MQ=255)
                               acGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCca                                                          >  1:3977478/1‑93 (MQ=255)
                               acGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCca                                                          >  1:2929285/1‑93 (MQ=255)
                                cGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCcaa                                                         <  2:83174/93‑1 (MQ=255)
                                cGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCcaa                                                         >  1:176094/1‑93 (MQ=255)
                                 ggCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCcaac                                                        <  2:121480/93‑1 (MQ=255)
                                  gCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCcaaca                                                       >  1:26983/1‑93 (MQ=255)
                                     aGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAAtt                                                    >  2:2769133/1‑93 (MQ=255)
                                     aGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAAtt                                                    >  2:591989/1‑93 (MQ=255)
                                      gACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTc                                                   >  1:4500133/1‑93 (MQ=255)
                                        ccGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCca                                                 <  1:1155414/93‑1 (MQ=255)
                                        ccGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCca                                                 <  1:1943034/93‑1 (MQ=255)
                                         cGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAACCCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCcac                                                <  2:3568739/93‑1 (MQ=255)
                                           gCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCcacac                                              >  1:3570228/1‑93 (MQ=255)
                                             agagTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACAt                                            >  2:3907593/1‑93 (MQ=255)
                                             agagTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACAt                                            <  2:1937453/93‑1 (MQ=255)
                                              gagTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTCCATCACGTTGCGCGATCGCGCCAACAATTCCACACATa                                           <  2:4209093/93‑1 (MQ=255)
                                              gagTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATa                                           >  1:540602/1‑93 (MQ=255)
                                              gagTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATa                                           <  2:1749906/93‑1 (MQ=255)
                                              gagTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATa                                           >  1:951210/1‑93 (MQ=255)
                                              gagTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATa                                           >  1:808331/1‑93 (MQ=255)
                                               agTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAg                                          <  1:1163066/93‑1 (MQ=255)
                                                 tCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGtt                                        <  1:2026736/93‑1 (MQ=255)
                                                 tCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGtt                                        >  2:2098322/1‑93 (MQ=255)
                                                  cATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGttt                                       >  2:143041/1‑93 (MQ=255)
                                                  cATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGttt                                       <  2:2570948/93‑1 (MQ=255)
                                                   atatCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGtttt                                      <  1:4633447/93‑1 (MQ=255)
                                                   atatCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGtttt                                      <  2:2924583/93‑1 (MQ=255)
                                                   atatCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGtttt                                      >  1:2228348/1‑93 (MQ=255)
                                                    tatCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGttttt                                     >  2:437219/1‑93 (MQ=255)
                                                      tCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGa                                   <  2:3258827/93‑1 (MQ=255)
                                                       ccGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGAt                                  <  1:3332231/93‑1 (MQ=255)
                                                        cgcgGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGAtt                                 <  1:3391448/93‑1 (MQ=255)
                                                         gcgGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTc                                <  2:1902123/93‑1 (MQ=255)
                                                         gcgGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTc                                <  2:311625/93‑1 (MQ=255)
                                                         gcgGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTc                                <  2:4311461/93‑1 (MQ=255)
                                                          cgGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTcc                               <  1:1489969/93‑1 (MQ=255)
                                                          cgGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTcc                               <  2:2016417/93‑1 (MQ=255)
                                                            gTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGa                             <  1:2757048/93‑1 (MQ=255)
                                                            gTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGa                             >  2:2718398/1‑93 (MQ=255)
                                                                 ccAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTtat                        <  1:780803/93‑1 (MQ=255)
                                                                  cAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTtata                       <  2:1463542/93‑1 (MQ=255)
                                                                   aGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTatat                      <  1:732288/93‑1 (MQ=255)
                                                                    gacgacGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATc                     >  2:3422027/1‑93 (MQ=255)
                                                                    gacgacGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATc                     <  2:2878471/93‑1 (MQ=255)
                                                                      cgacgTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGt                   <  2:4492271/93‑1 (MQ=255)
                                                                        acgTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTg                 >  2:2298443/1‑93 (MQ=255)
                                                                        acgTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCAAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTg                 <  2:3803544/93‑1 (MQ=255)
                                                                          gTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTc               >  1:4309086/1‑93 (MQ=255)
                                                                          gTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTc               <  1:2210924/93‑1 (MQ=255)
                                                                           tAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCg              >  2:1009387/1‑93 (MQ=255)
                                                                            aaaCCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCgg             <  1:3743756/93‑1 (MQ=255)
                                                                             aaCCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGt            >  2:3496633/1‑93 (MQ=255)
                                                                             aaCCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGt            <  2:3405947/93‑1 (MQ=255)
                                                                              aCCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTc           >  1:3877636/1‑93 (MQ=255)
                                                                                cTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCaa         <  1:3823367/93‑1 (MQ=255)
                                                                                 ttCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAAc        >  2:4671410/1‑93 (MQ=255)
                                                                                     agaagaATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGt    >  2:2652093/1‑93 (MQ=255)
                                                                                      gaagaaTTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTa   <  2:3713934/93‑1 (MQ=255)
                                                                                      gaagaaTTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTa   <  2:3326581/93‑1 (MQ=255)
                                                                                      gaagaaTTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTa   >  2:1716749/1‑93 (MQ=255)
                                                                                      gaagaaTTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTa   >  1:4025803/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTAT  >  NC_000913/3911159‑3911338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATG  >  NC_000913/3911158‑3911339
                                                                                           |                                                                                          
GCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAAT                                                                                          <  SRR4302535.1000328/94‑1 (MQ=60)
GCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAAT                                                                                          <  SRR4302535.570570/94‑1 (MQ=60)
 CAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATT                                                                                         <  SRR4302535.3304613/94‑1 (MQ=60)
 CAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATT                                                                                         <  SRR4302535.499407/94‑1 (MQ=60)
  AGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTT                                                                                        >  SRR4302535.240557/1‑94 (MQ=60)
  AGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTT                                                                                        <  SRR4302535.234075/94‑1 (MQ=60)
  AGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTT                                                                                        <  SRR4302535.1437933/94‑1 (MQ=60)
    GCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCT                                                                                      <  SRR4302535.87717/94‑1 (MQ=60)
     CGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTG                                                                                     <  SRR4302535.2185862/94‑1 (MQ=60)
     CGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTG                                                                                     >  SRR4302535.4237951/1‑94 (MQ=60)
      GGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGC                                                                                    >  SRR4302535.3289305/1‑94 (MQ=60)
      GGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGC                                                                                    >  SRR4302535.3885156/1‑94 (MQ=60)
       GGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCT                                                                                   <  SRR4302535.1072583/94‑1 (MQ=60)
       GGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCT                                                                                   <  SRR4302535.2348900/94‑1 (MQ=60)
        GTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTA                                                                                  <  SRR4302535.777147/94‑1 (MQ=60)
        GTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTA                                                                                  <  SRR4302535.4527695/94‑1 (MQ=60)
        GTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTA                                                                                  >  SRR4302535.1086657/1‑94 (MQ=60)
         TCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTAC                                                                                 <  SRR4302535.2482120/94‑1 (MQ=60)
          CATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACA                                                                                >  SRR4302535.1607373/1‑94 (MQ=60)
            TATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATC                                                                              >  SRR4302535.872106/1‑94 (MQ=60)
            TATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATC                                                                              <  SRR4302535.1693525/94‑1 (MQ=60)
             ATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCA                                                                             <  SRR4302535.3075296/94‑1 (MQ=60)
             ATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCA                                                                             <  SRR4302535.1553879/94‑1 (MQ=60)
              TGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCAC                                                                            <  SRR4302535.2239402/94‑1 (MQ=60)
              TGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCAC                                                                            <  SRR4302535.114510/94‑1 (MQ=60)
                ACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGT                                                                          >  SRR4302535.4154582/1‑94 (MQ=60)
                 CCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTT                                                                         <  SRR4302535.2737527/94‑1 (MQ=60)
                  CTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTG                                                                        <  SRR4302535.2541024/94‑1 (MQ=60)
                   TTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGC                                                                       <  SRR4302535.3423391/94‑1 (MQ=60)
                     CTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGC                                                                     >  SRR4302535.1879216/1‑94 (MQ=60)
                     CTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGC                                                                     >  SRR4302535.867386/1‑94 (MQ=60)
                      TGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCG                                                                    >  SRR4302535.2608734/1‑94 (MQ=60)
                      TGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCG                                                                    >  SRR4302535.3757991/1‑94 (MQ=60)
                        CATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGAT                                                                  <  SRR4302535.1105116/94‑1 (MQ=60)
                        CATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGAT                                                                  <  SRR4302535.4490405/94‑1 (MQ=60)
                         ATNAACNACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATC                                                                 <  SRR4302535.1280679/94‑1 (MQ=60)
                         ATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTGCAGACGCCGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATC                                                                 <  SRR4302535.3998843/94‑1 (MQ=60)
                         ATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATC                                                                 <  SRR4302535.1439230/94‑1 (MQ=60)
                          TCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCG                                                                <  SRR4302535.4594761/94‑1 (MQ=60)
                          TCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCG                                                                <  SRR4302535.474841/94‑1 (MQ=60)
                          TCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCG                                                                <  SRR4302535.474842/94‑1 (MQ=60)
                           CAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGC                                                               >  SRR4302535.3503521/1‑94 (MQ=60)
                           CAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGC                                                               >  SRR4302535.348339/1‑94 (MQ=60)
                            AACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCG                                                              <  SRR4302535.2013190/94‑1 (MQ=60)
                            AACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCG                                                              >  SRR4302535.4389478/1‑94 (MQ=60)
                             ACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGC                                                             >  SRR4302535.3951109/1‑94 (MQ=60)
                               AACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCA                                                           >  SRR4302535.4004652/1‑94 (MQ=60)
                               AACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCA                                                           >  SRR4302535.2949924/1‑94 (MQ=60)
                                ACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAA                                                          >  SRR4302535.177319/1‑94 (MQ=60)
                                ACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAA                                                          <  SRR4302535.1767561/94‑1 (MQ=60)
                                  GGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACA                                                        >  SRR4302535.27195/1‑94 (MQ=60)
                                    CCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACGAT                                                      >  SRR4302535.86756/1‑94 (MQ=60)
                                    CCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAAT                                                      <  SRR4302535.4049819/94‑1 (MQ=60)
                                    CCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAAT                                                      <  SRR4302535.3304202/94‑1 (MQ=60)
                                     CAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATT                                                     <  SRR4302535.1961970/94‑1 (MQ=60)
                                      AGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTC                                                    >  SRR4302535.4531039/1‑94 (MQ=60)
                                         CCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCAC                                                 <  SRR4302535.1163672/94‑1 (MQ=60)
                                         CCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCAC                                                 <  SRR4302535.1957030/94‑1 (MQ=60)
                                         ACGGCAGAGTCATATCCGCGGTATTCCAGACGCCGTAAGCCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCAC                                                 <  SRR4302535.2320988/94‑1 (MQ=60)
                                          CGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACA                                                <  SRR4302535.3771138/94‑1 (MQ=60)
                                           GGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACAC                                               >  SRR4302535.3594985/1‑94 (MQ=60)
                                            GCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACA                                              <  SRR4302535.994555/94‑1 (MQ=60)
                                             CAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACAT                                             <  SRR4302535.570775/94‑1 (MQ=60)
                                              AGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATA                                            >  SRR4302535.957942/1‑94 (MQ=60)
                                              AGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATA                                            >  SRR4302535.813991/1‑94 (MQ=60)
                                              AGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATA                                            >  SRR4302535.544376/1‑94 (MQ=60)
                                               GAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAG                                           <  SRR4302535.121880/94‑1 (MQ=60)
                                                AGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGT                                          >  SRR4302535.896056/1‑94 (MQ=60)
                                                AGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGT                                          <  SRR4302535.1171376/94‑1 (MQ=60)
                                                 GTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTT                                         <  SRR4302535.1105281/94‑1 (MQ=60)
                                                  TCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTT                                        <  SRR4302535.2041338/94‑1 (MQ=60)
                                                   CATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTT                                       >  SRR4302535.2244199/1‑94 (MQ=60)
                                                   CATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTT                                       >  SRR4302535.1632595/1‑94 (MQ=60)
                                                    ATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTT                                      >  SRR4302535.2190401/1‑94 (MQ=60)
                                                    ATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTT                                      <  SRR4302535.4664880/94‑1 (MQ=60)
                                                     TATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTG                                     <  SRR4302535.2715985/94‑1 (MQ=60)
                                                     TATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTG                                     <  SRR4302535.3804604/94‑1 (MQ=60)
                                                      ATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGA                                    <  SRR4302535.780010/94‑1 (MQ=60)
                                                        CCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATT                                  <  SRR4302535.3355422/94‑1 (MQ=60)
                                                         CGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTC                                 <  SRR4302535.1217692/94‑1 (MQ=60)
                                                         CGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTC                                 >  SRR4302535.4398707/1‑94 (MQ=60)
                                                         CGCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTC                                 <  SRR4302535.3415042/94‑1 (MQ=60)
                                                          GCGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCC                                <  SRR4302535.3435981/94‑1 (MQ=60)
                                                           CGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCG                               <  SRR4302535.4125926/94‑1 (MQ=60)
                                                           CGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCG                               <  SRR4302535.2228330/94‑1 (MQ=60)
                                                           CGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCG                               >  SRR4302535.2386718/1‑94 (MQ=60)
                                                           CGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCG                               <  SRR4302535.1500665/94‑1 (MQ=60)
                                                           CGGTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCG                               <  SRR4302535.4682017/94‑1 (MQ=60)
                                                             GTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGAT                             <  SRR4302535.2776697/94‑1 (MQ=60)
                                                             GTATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGAT                             <  SRR4302535.1281151/94‑1 (MQ=60)
                                                              TATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATT                            <  SRR4302535.1165458/94‑1 (MQ=60)
                                                               ATTCCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTT                           >  SRR4302535.2713052/1‑94 (MQ=60)
                                                                  CCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATA                        <  SRR4302535.786286/94‑1 (MQ=60)
                                                                  CCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATA                        >  SRR4302535.1912210/1‑94 (MQ=60)
                                                                  CCAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGCTTTTGATTCCGATTTATA                        >  SRR4302535.2331351/1‑94 (MQ=60)
                                                                   CAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATAT                       >  SRR4302535.285715/1‑94 (MQ=60)
                                                                   CAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATAT                       >  SRR4302535.3859536/1‑94 (MQ=60)
                                                                   CAGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATAT                       <  SRR4302535.790340/94‑1 (MQ=60)
                                                                    AGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATC                      >  SRR4302535.3564359/1‑94 (MQ=60)
                                                                    AGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATC                      <  SRR4302535.737429/94‑1 (MQ=60)
                                                                    AGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATC                      <  SRR4302535.536072/94‑1 (MQ=60)
                                                                    AGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATC                      >  SRR4302535.491470/1‑94 (MQ=60)
                                                                    AGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATC                      <  SRR4302535.1672676/94‑1 (MQ=60)
                                                                    AGACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATC                      >  SRR4302535.4018071/1‑94 (MQ=60)
                                                                     GACGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCG                     <  SRR4302535.411545/94‑1 (MQ=60)
                                                                       CGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTT                   >  SRR4302535.703809/1‑94 (MQ=60)
                                                                       CGACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTT                   >  SRR4302535.2541138/1‑94 (MQ=60)
                                                                         ACGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGT                 >  SRR4302535.4519550/1‑94 (MQ=60)
                                                                          CGTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTC                >  SRR4302535.4338566/1‑94 (MQ=60)
                                                                           GTAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCG               <  SRR4302535.2226674/94‑1 (MQ=60)
                                                                            TAAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGG              >  SRR4302535.3035246/1‑94 (MQ=60)
                                                                             AAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGT             <  SRR4302535.2217825/94‑1 (MQ=60)
                                                                             AAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGT             <  SRR4302535.3769657/94‑1 (MQ=60)
                                                                             AAACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGT             >  SRR4302535.1586979/1‑94 (MQ=60)
                                                                              AACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTC            >  SRR4302535.4680299/1‑94 (MQ=60)
                                                                              AACCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTC            >  SRR4302535.3904265/1‑94 (MQ=60)
                                                                                CCTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAA          >  SRR4302535.2044721/1‑94 (MQ=60)
                                                                                 CTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAAC         >  SRR4302535.3394232/1‑94 (MQ=60)
                                                                                 CTTCAAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAAC         <  SRR4302535.3849738/94‑1 (MQ=60)
                                                                                     AAGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGT     <  SRR4302535.2760764/94‑1 (MQ=60)
                                                                                      AGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTA    <  SRR4302535.1326036/94‑1 (MQ=60)
                                                                                      AGAAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTA    >  SRR4302535.4053348/1‑94 (MQ=60)
                                                                                        AAGAATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATG  <  SRR4302535.2438693/94‑1 (MQ=60)
                                                                                           |                                                                                          
GCAGGCGGGTCATATGACCTTCTGCATCAACAACGGCCAGACCGGCAGAGTCATATCCGCGGTATTCCAGACGACGTAAACCTTCAAGAAGGATTTCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATG  >  NC_000913/3911158‑3911339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑