Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,916,382 A→G V368V (GTT→GTC)  atpA ← membrane‑bound ATP synthase, F1 sector, alpha‑subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,916,3820AG100.0% 812.3 / NA 258V368V (GTT→GTC) atpAmembrane‑bound ATP synthase, F1 sector, alpha‑subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (122/136);  total (122/136)

Only 100 of 267 total aligned reads displayed.

TGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGC  >  NC_000913/3916292‑3916471
                                                                                          |                                                                                         
tgCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGAc                                                                                         <  2:1254778/93‑1 (MQ=255)
 gCCAGAGCGGTACGGATACCCCCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGAcc                                                                                        >  2:3899499/1‑93 (MQ=255)
 gCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGAcc                                                                                        >  1:308094/1‑93 (MQ=255)
  ccAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCg                                                                                       >  2:3514434/1‑93 (MQ=255)
  ccAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCg                                                                                       >  2:2464667/1‑93 (MQ=255)
   cAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGc                                                                                      >  1:2376275/1‑93 (MQ=255)
   cAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGc                                                                                      >  2:801551/1‑93 (MQ=255)
    agagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCa                                                                                     >  1:2041128/1‑93 (MQ=255)
     gagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAg                                                                                    >  1:3607619/1‑93 (MQ=255)
     gagCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAg                                                                                    <  1:1467049/93‑1 (MQ=255)
       gCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGa                                                                                  <  1:4103991/93‑1 (MQ=255)
          gTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGa                                                                               >  1:3179334/1‑93 (MQ=255)
            aCGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAAt                                                                             <  2:2515941/93‑1 (MQ=255)
             cGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATa                                                                            <  1:259287/93‑1 (MQ=255)
              ggATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATAc                                                                           <  2:2052161/93‑1 (MQ=255)
              ggATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATAc                                                                           <  2:3281387/93‑1 (MQ=255)
               gATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATAcc                                                                          <  2:2556136/93‑1 (MQ=255)
               gATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATAcc                                                                          >  2:20017/1‑93 (MQ=255)
                 tACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCgg                                                                        >  1:4654739/1‑93 (MQ=255)
                    caccGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGt                                                                     >  2:3466301/1‑93 (MQ=255)
                    caccGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGt                                                                     >  1:1163195/1‑93 (MQ=255)
                    caccGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGt                                                                     <  1:4316194/93‑1 (MQ=255)
                     accGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGtt                                                                    >  1:3127254/1‑93 (MQ=255)
                      ccGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTg                                                                   <  1:4382594/93‑1 (MQ=255)
                      ccGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTg                                                                   <  2:4238920/93‑1 (MQ=255)
                        ggACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGaa                                                                 >  2:130652/1‑93 (MQ=255)
                         gACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATCCCCGGGTTGACCGCAGGACGAATACCGGCGTTGAAc                                                                <  1:1065597/93‑1 (MQ=255)
                         gACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAAc                                                                >  2:2332874/1‑93 (MQ=255)
                          aCAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACa                                                               <  1:3960815/93‑1 (MQ=255)
                          aCAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACa                                                               <  2:942381/93‑1 (MQ=255)
                           cAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAg                                                              >  2:2112228/1‑93 (MQ=255)
                           cAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAg                                                              <  1:1863046/93‑1 (MQ=255)
                            aGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAgg                                                             >  1:805384/1‑93 (MQ=255)
                             gTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAggt                                                            <  2:3155886/93‑1 (MQ=255)
                              ttttttCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAggtt                                                           >  2:719741/1‑93 (MQ=255)
                              ttttttCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAggtt                                                           <  1:728840/93‑1 (MQ=255)
                              ttttttCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAggtt                                                           <  1:2871743/93‑1 (MQ=255)
                              ttttttCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGGTGACCGCAGGACGAATACCGGCGTTGAACAggct                                                           >  1:1549971/1‑91 (MQ=255)
                               tttttCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCTGGGTTGACCGCAGGACGAATACCGGCGTTGAACAggttg                                                          >  1:3500416/1‑93 (MQ=255)
                                ttttCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAggttgg                                                         <  2:36918/93‑1 (MQ=255)
                                ttttCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAggttgg                                                         >  1:3840392/1‑93 (MQ=255)
                                 tttCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAggttggt                                                        <  1:2419421/93‑1 (MQ=255)
                                   tCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGttt                                                      >  2:4148773/1‑93 (MQ=255)
                                    cATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTc                                                     >  1:2864115/1‑93 (MQ=255)
                                      tGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCa                                                   >  2:2627818/1‑93 (MQ=255)
                                      tGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCa                                                   <  1:2710098/93‑1 (MQ=255)
                                      tGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCa                                                   <  2:2244541/93‑1 (MQ=255)
                                      tGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCa                                                   <  2:3107838/93‑1 (MQ=255)
                                        aTCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAgg                                                 >  1:2962849/1‑93 (MQ=255)
                                        aTCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAgg                                                 >  2:1898017/1‑93 (MQ=255)
                                         tCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGa                                                >  2:149775/1‑93 (MQ=255)
                                         tCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGa                                                >  2:3821504/1‑93 (MQ=255)
                                         tCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGa                                                <  2:1768653/93‑1 (MQ=255)
                                         tCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGa                                                >  1:2761409/1‑93 (MQ=255)
                                         tCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGa                                                >  1:4124745/1‑93 (MQ=255)
                                          cTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGaa                                               <  1:1784376/93‑1 (MQ=255)
                                           ttGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAg                                              <  2:1231914/93‑1 (MQ=255)
                                           ttGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAg                                              <  1:622537/93‑1 (MQ=255)
                                              gTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATc                                           <  2:4354437/93‑1 (MQ=255)
                                              gTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATc                                           <  2:2879963/93‑1 (MQ=255)
                                              gTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATc                                           >  2:3221966/1‑93 (MQ=255)
                                               tCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCt                                          <  2:1048132/93‑1 (MQ=255)
                                                    gctgcACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCa                                     >  2:4526896/1‑93 (MQ=255)
                                                     ctgcACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAt                                    >  1:1173952/1‑93 (MQ=255)
                                                      tgcACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATc                                   <  1:1408365/93‑1 (MQ=255)
                                                        caccacCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCgg                                 >  1:662794/1‑93 (MQ=255)
                                                         cccaccaACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGt                                <  1:1286058/92‑1 (MQ=255)
                                                         accaccAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGt                                >  2:2194099/1‑93 (MQ=255)
                                                           caccaACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTaa                              <  1:3838269/93‑1 (MQ=255)
                                                            accaACACGGGATACGGAAATACCCGGGTTGCCCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCAGTAAt                             <  2:4681131/93‑1 (MQ=255)
                                                            accaACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAAt                             >  1:238225/1‑93 (MQ=255)
                                                            accaACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAAt                             >  1:1974716/1‑93 (MQ=255)
                                                             ccaACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATg                            >  2:3604278/1‑93 (MQ=255)
                                                             ccaACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATg                            <  2:1099438/93‑1 (MQ=255)
                                                               aaCACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGga                          >  2:1346825/1‑93 (MQ=255)
                                                               aaCACGGGATACGGAAATACCCGGGTGGACCGCAGGACGAATACCGGCGTTGAACAGGTTGTTTTCCAGGAAGATCTGACCATCGGTAATGga                          <  2:3354059/93‑1 (MQ=255)
                                                                acacGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGgag                         <  1:33478/93‑1 (MQ=255)
                                                                acacGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGgag                         >  1:1013002/1‑93 (MQ=255)
                                                                acacGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGgag                         >  1:451715/1‑93 (MQ=255)
                                                                 cacGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGgaga                        <  2:4652469/93‑1 (MQ=255)
                                                                 cacGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGgaga                        <  1:985609/93‑1 (MQ=255)
                                                                 cacGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGgaga                        <  1:417503/93‑1 (MQ=255)
                                                                   cGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGAtt                      >  2:3493133/1‑93 (MQ=255)
                                                                   cGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGAtt                      >  1:4252499/1‑93 (MQ=255)
                                                                    gggATACGGAAATACCCGGGGTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTa                     <  2:398514/93‑1 (MQ=255)
                                                                     ggATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTAc                    <  1:2934717/93‑1 (MQ=255)
                                                                      gATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACg                   >  2:4309823/1‑93 (MQ=255)
                                                                           ggAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTCCGTTGGt              >  1:2995461/1‑93 (MQ=255)
                                                                           ggAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGt              >  2:1717539/1‑93 (MQ=255)
                                                                           ggAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGt              <  2:2995345/93‑1 (MQ=255)
                                                                           ggAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGt              >  2:646643/1‑93 (MQ=255)
                                                                            gAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTc             >  1:604182/1‑93 (MQ=255)
                                                                            gAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTc             >  1:4592072/1‑93 (MQ=255)
                                                                            gAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTc             <  1:3236638/93‑1 (MQ=255)
                                                                             aaaTACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCg            <  1:1688483/93‑1 (MQ=255)
                                                                              aaTACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCgg           >  2:1676306/1‑93 (MQ=255)
                                                                              aaTACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCgg           <  1:1701347/93‑1 (MQ=255)
                                                                                tACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGgaa         <  2:2447754/93‑1 (MQ=255)
                                                                                     gggTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGgaacgaac    >  2:1394007/1‑93 (MQ=255)
                                                                                     gggTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGgaacgaac    <  1:1029281/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGC  >  NC_000913/3916292‑3916471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAA  >  NC_000913/3916292‑3916475
                                                                                          |                                                                                             
TGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACC                                                                                            >  SRR4302535.310227/1‑94 (MQ=60)
  CCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGC                                                                                          <  SRR4302535.4433310/94‑1 (MQ=60)
  CCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGC                                                                                          >  SRR4302535.2393345/1‑94 (MQ=60)
   CAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCA                                                                                         >  SRR4302535.2055828/1‑94 (MQ=60)
   CAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCA                                                                                         >  SRR4302535.2726167/1‑94 (MQ=60)
    AGAGCGGTACGGATTCCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACTACCAACACGGGATACGGAAATACCCGGGTTGACCGCAG                                                                                        <  SRR4302535.4668581/94‑1 (MQ=60)
    AGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAG                                                                                        >  SRR4302535.4208520/1‑94 (MQ=60)
    AGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAG                                                                                        >  SRR4302535.3632639/1‑94 (MQ=60)
    AGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAG                                                                                        >  SRR4302535.3325148/1‑94 (MQ=60)
     GAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGG                                                                                       <  SRR4302535.1477557/94‑1 (MQ=60)
      AGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGA                                                                                      >  SRR4302535.3980082/1‑94 (MQ=60)
      AGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGA                                                                                      >  SRR4302535.3617838/1‑94 (MQ=60)
       GCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGAC                                                                                     <  SRR4302535.4185896/94‑1 (MQ=60)
       GCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGAC                                                                                     <  SRR4302535.4132086/94‑1 (MQ=60)
        CGGTACGGATACCGCCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACG                                                                                    <  SRR4302535.291947/94‑1 (MQ=60)
         GGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGA                                                                                   >  SRR4302535.3055417/1‑94 (MQ=60)
         GGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGA                                                                                   >  SRR4302535.3201316/1‑94 (MQ=60)
          GTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAA                                                                                  <  SRR4302535.3869090/94‑1 (MQ=60)
           TACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAAT                                                                                 <  SRR4302535.2423564/94‑1 (MQ=60)
             CGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATAC                                                                               <  SRR4302535.261081/94‑1 (MQ=60)
              GGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACC                                                                              >  SRR4302535.3810849/1‑94 (MQ=60)
               GATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCG                                                                             <  SRR4302535.1121157/94‑1 (MQ=60)
                ATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGG                                                                            >  SRR4302535.4686246/1‑94 (MQ=60)
                  ACNACCNNACAGTTTTTTCATNNTCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCG                                                                          <  SRR4302535.4123453/94‑1 (MQ=60)
                  ACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCG                                                                          >  SRR4302535.2844181/1‑94 (MQ=60)
                   CCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGT                                                                         >  SRR4302535.2750552/1‑94 (MQ=60)
                   CCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGT                                                                         >  SRR4302535.1171505/1‑94 (MQ=60)
                    CACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTT                                                                        <  SRR4302535.4345720/94‑1 (MQ=60)
                    CACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTT                                                                        >  SRR4302535.3129566/1‑94 (MQ=60)
                    CACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTT                                                                        >  SRR4302535.3149034/1‑94 (MQ=60)
                    CACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTT                                                                        <  SRR4302535.781426/94‑1 (MQ=60)
                     ACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTG                                                                       <  SRR4302535.2008692/94‑1 (MQ=60)
                      CCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGA                                                                      <  SRR4302535.4412577/94‑1 (MQ=60)
                        GGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAAC                                                                    <  SRR4302535.3010670/94‑1 (MQ=60)
                        GGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAAC                                                                    >  SRR4302535.3621696/1‑94 (MQ=60)
                         GACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATCCCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACA                                                                   <  SRR4302535.1073177/94‑1 (MQ=60)
                         GACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACA                                                                   >  SRR4302535.117725/1‑94 (MQ=60)
                          ACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAG                                                                  >  SRR4302535.430589/1‑94 (MQ=60)
                          ACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAG                                                                  <  SRR4302535.3987864/94‑1 (MQ=60)
                           CAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGG                                                                 <  SRR4302535.1876480/94‑1 (MQ=60)
                           CAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGG                                                                 >  SRR4302535.811035/1‑94 (MQ=60)
                            AGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGT                                                                >  SRR4302535.100324/1‑94 (MQ=60)
                            AGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGT                                                                <  SRR4302535.1258249/94‑1 (MQ=60)
                             GTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTT                                                               <  SRR4302535.719655/94‑1 (MQ=60)
                             GTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGGTGACCGCAGGACGAATACCGGCGTTGAACAGGCT                                                               >  SRR4302535.1561136/1‑94 (MQ=60)
                              TTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCTGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTG                                                              >  SRR4302535.3524753/1‑94 (MQ=60)
                              TTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTG                                                              <  SRR4302535.733953/94‑1 (MQ=60)
                              TTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTG                                                              <  SRR4302535.2892094/94‑1 (MQ=60)
                              TTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTG                                                              <  SRR4302535.1815484/94‑1 (MQ=60)
                               TTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGG                                                             >  SRR4302535.3866856/1‑94 (MQ=60)
                                 TTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTT                                                           <  SRR4302535.3291502/94‑1 (MQ=60)
                                 TTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTT                                                           <  SRR4302535.2436842/94‑1 (MQ=60)
                                   TCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTC                                                         >  SRR4302535.2884409/1‑94 (MQ=60)
                                      TGATCTTGGTCTGTGCTGCACCACCAACACGGGATGCGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAG                                                      <  SRR4302535.2134219/94‑1 (MQ=60)
                                      TGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAG                                                      <  SRR4302535.2729469/94‑1 (MQ=60)
                                       GATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGG                                                     >  SRR4302535.2983647/1‑94 (MQ=60)
                                       GATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGG                                                     >  SRR4302535.1513750/1‑94 (MQ=60)
                                       GATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGA                                                     >  SRR4302535.1038647/1‑94 (MQ=60)
                                       GATCTTGGTCGGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGG                                                     <  SRR4302535.3219460/94‑1 (MQ=60)
                                        ATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGA                                                    >  SRR4302535.2781085/1‑94 (MQ=60)
                                        ATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGA                                                    <  SRR4302535.1445117/94‑1 (MQ=60)
                                        ATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGA                                                    >  SRR4302535.4152973/1‑94 (MQ=60)
                                         TCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAA                                                   >  SRR4302535.2887447/1‑94 (MQ=60)
                                         TCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAA                                                   <  SRR4302535.2477530/94‑1 (MQ=60)
                                         TCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAA                                                   <  SRR4302535.4194960/94‑1 (MQ=60)
                                          CTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAG                                                  >  SRR4302535.2731418/1‑94 (MQ=60)
                                          CTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAG                                                  >  SRR4302535.4262961/1‑94 (MQ=60)
                                          CTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAG                                                  <  SRR4302535.1797292/94‑1 (MQ=60)
                                          CTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAG                                                  <  SRR4302535.4647806/94‑1 (MQ=60)
                                           TTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGA                                                 >  SRR4302535.4427873/1‑94 (MQ=60)
                                           TTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGA                                                 <  SRR4302535.626861/94‑1 (MQ=60)
                                            TGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGAT                                                <  SRR4302535.4372418/94‑1 (MQ=60)
                                            TGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGAT                                                <  SRR4302535.4138866/94‑1 (MQ=60)
                                             GGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATC                                               <  SRR4302535.3134121/94‑1 (MQ=60)
                                              GTCTGTGCTGCACCACCAACACGGGATACGGAAATGCCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCT                                              <  SRR4302535.1931708/94‑1 (MQ=60)
                                              GTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCT                                              <  SRR4302535.2979241/94‑1 (MQ=60)
                                              GTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCT                                              >  SRR4302535.1201097/1‑94 (MQ=60)
                                                   TGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCA                                         >  SRR4302535.638084/1‑94 (MQ=60)
                                                    GCTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCAT                                        >  SRR4302535.1182332/1‑94 (MQ=60)
                                                     CTGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATC                                       <  SRR4302535.3433293/94‑1 (MQ=60)
                                                      TGCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCG                                      <  SRR4302535.1418425/94‑1 (MQ=60)
                                                       GCACCACCAACACGGGATACGGAAATACCCTGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGG                                     <  SRR4302535.2399294/94‑1 (MQ=60)
                                                       GCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGG                                     <  SRR4302535.1703910/94‑1 (MQ=60)
                                                       GCACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGG                                     >  SRR4302535.667422/1‑94 (MQ=60)
                                                        CACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGT                                    <  SRR4302535.614384/94‑1 (MQ=60)
                                                        CACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGT                                    <  SRR4302535.3198458/94‑1 (MQ=60)
                                                         CCCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTA                                   <  SRR4302535.1295225/94‑1 (MQ=60)
                                                         ACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTA                                   >  SRR4302535.4023497/1‑94 (MQ=60)
                                                         ACCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCCGTA                                   >  SRR4302535.1036587/1‑94 (MQ=60)
                                                          CCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCGTCGGTAA                                  >  SRR4302535.1144392/1‑94 (MQ=60)
                                                          CCACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAA                                  >  SRR4302535.1727042/1‑94 (MQ=60)
                                                           CACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAAT                                 >  SRR4302535.1988939/1‑94 (MQ=60)
                                                           CACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAAT                                 >  SRR4302535.239862/1‑94 (MQ=60)
                                                           CACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAAT                                 <  SRR4302535.3864727/94‑1 (MQ=60)
                                                            ACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATG                                <  SRR4302535.2975881/94‑1 (MQ=60)
                                                            ACCAACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATG                                <  SRR4302535.63842/94‑1 (MQ=60)
                                                               AACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAG                             >  SRR4302535.1020206/1‑94 (MQ=60)
                                                               AACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAG                             >  SRR4302535.454877/1‑94 (MQ=60)
                                                               AACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAG                             <  SRR4302535.164428/94‑1 (MQ=60)
                                                                ACACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGA                            <  SRR4302535.33734/94‑1 (MQ=60)
                                                                 CACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGAT                           <  SRR4302535.992610/94‑1 (MQ=60)
                                                                 CACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGAT                           <  SRR4302535.420429/94‑1 (MQ=60)
                                                                 CACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGAT                           <  SRR4302535.242491/94‑1 (MQ=60)
                                                                 CACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGCAATGGAGAT                           >  SRR4302535.553792/1‑94 (MQ=60)
                                                                  ACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATT                          <  SRR4302535.183374/94‑1 (MQ=60)
                                                                  ACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATT                          >  SRR4302535.2036667/1‑94 (MQ=60)
                                                                  ACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATT                          >  SRR4302535.4281636/1‑94 (MQ=60)
                                                                  ACGGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATT                          <  SRR4302535.2566963/94‑1 (MQ=60)
                                                                    GGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTAC                        <  SRR4302535.3866177/94‑1 (MQ=60)
                                                                    GGGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTAC                        <  SRR4302535.4409369/94‑1 (MQ=60)
                                                                     GGATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACG                       <  SRR4302535.2955371/94‑1 (MQ=60)
                                                                      GATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGT                      <  SRR4302535.2880073/94‑1 (MQ=60)
                                                                       ATACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTT                     >  SRR4302535.3830492/1‑94 (MQ=60)
                                                                        TACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTG                    <  SRR4302535.1607320/94‑1 (MQ=60)
                                                                         ACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGG                   <  SRR4302535.925243/94‑1 (MQ=60)
                                                                         ACGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGG                   <  SRR4302535.534231/94‑1 (MQ=60)
                                                                          CGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGCACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGT                  <  SRR4302535.1339276/94‑1 (MQ=60)
                                                                          CGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTCCGTTGGT                  >  SRR4302535.3016458/1‑94 (MQ=60)
                                                                          CGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGT                  <  SRR4302535.4618769/94‑1 (MQ=60)
                                                                          CGGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGT                  <  SRR4302535.1796648/94‑1 (MQ=60)
                                                                           GGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTC                 >  SRR4302535.4623331/1‑94 (MQ=60)
                                                                           GGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTC                 >  SRR4302535.608411/1‑94 (MQ=60)
                                                                           GGAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTC                 >  SRR4302535.3078409/1‑94 (MQ=60)
                                                                            GAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCG                <  SRR4302535.155480/94‑1 (MQ=60)
                                                                            GAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCG                <  SRR4302535.3247174/94‑1 (MQ=60)
                                                                            GAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCG                >  SRR4302535.2330792/1‑94 (MQ=60)
                                                                            GAAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCG                <  SRR4302535.3259106/94‑1 (MQ=60)
                                                                             AAATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGG               <  SRR4302535.1700706/94‑1 (MQ=60)
                                                                              AATACCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGA              <  SRR4302535.1713643/94‑1 (MQ=60)
                                                                                  CCCGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGA          <  SRR4302535.2905865/94‑1 (MQ=60)
                                                                                    CGGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAAC        <  SRR4302535.1057742/94‑1 (MQ=60)
                                                                                     GGGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACG       <  SRR4302535.1036588/94‑1 (MQ=60)
                                                                                      GGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGCACGAACGC      >  SRR4302535.894974/1‑94 (MQ=60)
                                                                                      GGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGC      <  SRR4302535.1403601/94‑1 (MQ=60)
                                                                                      GGTTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGC      >  SRR4302535.73018/1‑94 (MQ=60)
                                                                                        TTGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAG    <  SRR4302535.2426145/94‑1 (MQ=60)
                                                                                         TGACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGA   <  SRR4302535.3049804/94‑1 (MQ=60)
                                                                                          GACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAA  >  SRR4302535.2019931/1‑94 (MQ=60)
                                                                                          GACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAA  >  SRR4302535.2030849/1‑94 (MQ=60)
                                                                                          GACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAA  <  SRR4302535.1239638/94‑1 (MQ=60)
                                                                                          GACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAA  <  SRR4302535.3473788/94‑1 (MQ=60)
                                                                                          |                                                                                             
TGCCAGAGCGGTACGGATACCACCGGACAGTTTTTTCATGATCTTGGTCTGTGCTGCACCACCAACACGGGATACGGAAATACCCGGGTTAACCGCAGGACGAATACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAA  >  NC_000913/3916292‑3916475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑