Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,927,931 C→T A264T (GCC→ACC)  yieN ← putative 2‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,927,9310CT99.6% 798.7 / NA 232A264T (GCC→ACC) yieNputative 2‑component regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (111/119);  minor base G (1/0);  total (113/119)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 241 total aligned reads displayed.

TCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCA  >  NC_000913/3927841‑3928020
                                                                                          |                                                                                         
tCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGgtgc                                                                                         >  1:4645711/1‑93 (MQ=255)
 cAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCt                                                                                        <  1:4360182/93‑1 (MQ=255)
 cAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCt                                                                                        <  1:1518398/93‑1 (MQ=255)
  aaaTTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTg                                                                                       >  2:3408904/1‑93 (MQ=255)
  aaaTTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTg                                                                                       <  1:3588652/93‑1 (MQ=255)
   aaTTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTgg                                                                                      <  2:75651/93‑1 (MQ=255)
   aaTTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTgg                                                                                      <  1:1682776/93‑1 (MQ=255)
    aTTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGc                                                                                     >  2:1664896/1‑93 (MQ=255)
        aGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGATATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGc                                                                                 <  2:1268421/93‑1 (MQ=255)
        aGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGc                                                                                 >  1:2403967/1‑93 (MQ=255)
         ggCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCa                                                                                >  2:4176383/1‑93 (MQ=255)
          gCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCaa                                                                               >  2:1798539/1‑93 (MQ=255)
             ttGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCaataa                                                                            <  2:1590988/93‑1 (MQ=255)
             ttGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCaataa                                                                            <  1:4414058/93‑1 (MQ=255)
                cgcgTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCg                                                                         >  1:4206144/1‑93 (MQ=255)
                cgcgTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCg                                                                         >  1:2760438/1‑93 (MQ=255)
                  cgTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACATCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGa                                                                       <  1:2349669/93‑1 (MQ=255)
                  cgTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGa                                                                       <  2:2972601/93‑1 (MQ=255)
                   gTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGAt                                                                      >  1:1595968/1‑93 (MQ=255)
                   gTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGAt                                                                      >  1:4655161/1‑93 (MQ=255)
                    tCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATc                                                                     <  2:495594/93‑1 (MQ=255)
                      aTACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGc                                                                   <  1:1038138/93‑1 (MQ=255)
                       tACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCt                                                                  >  1:1914285/1‑93 (MQ=255)
                       tACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCt                                                                  >  2:3743352/1‑93 (MQ=255)
                       tACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCt                                                                  >  1:3141360/1‑93 (MQ=255)
                       tACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCt                                                                  >  2:1809465/1‑93 (MQ=255)
                        aCCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCtt                                                                 <  2:2768091/93‑1 (MQ=255)
                        aCCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCtt                                                                 >  1:4068476/1‑93 (MQ=255)
                         ccACAGTCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCttt                                                                <  1:2123821/93‑1 (MQ=255)
                         ccACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCttt                                                                <  2:1096075/93‑1 (MQ=255)
                          cacaGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCtttt                                                               <  2:4045749/93‑1 (MQ=255)
                          cacaGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCtttt                                                               <  2:1757881/93‑1 (MQ=255)
                           acaGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCttttt                                                              >  2:3779063/1‑93 (MQ=255)
                            caGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCtttttt                                                             <  2:3176289/93‑1 (MQ=255)
                            caGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCtttttt                                                             >  1:2156530/1‑93 (MQ=255)
                              ggCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTcc                                                           >  2:1005532/1‑93 (MQ=255)
                                cAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAg                                                         <  2:2915918/93‑1 (MQ=255)
                                cAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTCTTTTCCAg                                                         <  2:4612516/93‑1 (MQ=255)
                                cAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCCCTTTTTTCCAg                                                         <  2:2095397/93‑1 (MQ=255)
                                 aaTCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGc                                                        >  1:3355038/1‑93 (MQ=255)
                                 aaTCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGc                                                        <  1:3198516/93‑1 (MQ=255)
                                  aTCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGcg                                                       >  1:30470/1‑93 (MQ=255)
                                   tCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGcga                                                      <  1:2667435/93‑1 (MQ=255)
                                     tttCAGCACAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGcgacg                                                    >  2:4565464/1‑93 (MQ=255)
                                     tttCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGcgacg                                                    >  1:1824615/1‑93 (MQ=255)
                                     tttCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGcgacg                                                    >  1:184214/1‑93 (MQ=255)
                                       tCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGAt                                                  <  1:947354/93‑1 (MQ=255)
                                        cAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATc                                                 <  1:680607/93‑1 (MQ=255)
                                         aGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATcc                                                >  2:4234082/1‑93 (MQ=255)
                                            aaaaTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCgag                                             >  2:816053/1‑93 (MQ=255)
                                             aaaTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCgaga                                            <  1:2282123/93‑1 (MQ=255)
                                             aaaTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCgaga                                            <  1:2546063/93‑1 (MQ=255)
                                              aaTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGAc                                           >  2:2845620/1‑93 (MQ=255)
                                              aaTGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGACCCGAGAc                                           >  1:3418881/1‑93 (MQ=255)
                                                tGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACAt                                         >  2:1327323/1‑93 (MQ=255)
                                                tGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACAt                                         >  2:1899275/1‑93 (MQ=255)
                                                 gagaTCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATa                                        >  1:1402033/1‑93 (MQ=255)
                                                  cgaTCTACAGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATaa                                       <  1:4500156/92‑1 (MQ=255)
                                                  agaTCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATaa                                       >  2:3491909/1‑93 (MQ=255)
                                                  agaTCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATaa                                       <  1:3086315/93‑1 (MQ=255)
                                                  agaTCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATaa                                       <  2:1707047/93‑1 (MQ=255)
                                                  agaTCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATaa                                       <  1:37639/93‑1 (MQ=255)
                                                     tCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGgc                                    >  2:3206901/1‑93 (MQ=255)
                                                     tCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGgc                                    >  1:2217810/1‑93 (MQ=255)
                                                     tCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGgc                                    >  2:1221113/1‑93 (MQ=255)
                                                        aCCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCa                                 >  2:2449223/1‑93 (MQ=255)
                                                        aCCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCa                                 >  1:3949112/1‑93 (MQ=255)
                                                         ccGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAt                                <  1:1280048/93‑1 (MQ=255)
                                                         ccGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAt                                >  1:1573333/1‑93 (MQ=255)
                                                         ccGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAt                                >  1:3893837/1‑93 (MQ=255)
                                                         ccGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGACTGCAATAATCGGATCGCGTTTTTCCAGCGACGATCCGAGACACAAGGCGCAt                                >  1:2854694/1‑93 (MQ=255)
                                                           ggggCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATcc                              <  2:3917471/93‑1 (MQ=255)
                                                            gtgCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCg                             <  2:151432/91‑1 (MQ=255)
                                                              gCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGt                           <  1:603550/93‑1 (MQ=255)
                                                                aaCAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGCTCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTaa                         <  2:3547395/93‑1 (MQ=255)
                                                                aaCAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTaa                         >  2:3667582/1‑93 (MQ=255)
                                                                aaCAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTaa                         <  2:3361889/93‑1 (MQ=255)
                                                                 aCAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAAt                        <  1:2322086/93‑1 (MQ=255)
                                                                  cAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAAtt                       <  1:1969846/93‑1 (MQ=255)
                                                                    gCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTa                     <  2:957666/93‑1 (MQ=255)
                                                                    gCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTa                     >  1:2594867/1‑93 (MQ=255)
                                                                    gCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTa                     <  2:2247191/93‑1 (MQ=255)
                                                                    gCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTa                     >  1:2714638/1‑93 (MQ=255)
                                                                      aCTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATc                   >  1:819625/1‑93 (MQ=255)
                                                                      aCTGCGACCGCTAAAAAAGGGGCTGGTCTGCAATAATCGGATCGCTTTTTTCCAGCGAGGATCCGAGACATAAGGCGCATCCGGTAATTTATc                   >  1:2879113/1‑93 (MQ=255)
                                                                       cTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATcc                  <  2:3462406/93‑1 (MQ=255)
                                                                       cTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATcc                  >  2:4647015/1‑93 (MQ=255)
                                                                       cTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATcc                  >  1:2845522/1‑93 (MQ=255)
                                                                       cTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCAGAGACATAAGGCGCATCCGGTAATTTATcc                  >  2:1993317/1‑93 (MQ=255)
                                                                          cGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGt               >  2:3304354/1‑93 (MQ=255)
                                                                           gACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGtt              <  1:892106/93‑1 (MQ=255)
                                                                           gACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGtt              <  1:198043/93‑1 (MQ=255)
                                                                            aCCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTg             >  2:4458430/1‑93 (MQ=255)
                                                                             ccGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGc            <  2:3106578/93‑1 (MQ=255)
                                                                             ccGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGc            >  1:1094696/1‑93 (MQ=255)
                                                                               gCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTg          <  2:3946367/93‑1 (MQ=255)
                                                                                 tAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGgc        >  2:880697/1‑93 (MQ=255)
                                                                                   aaaaaGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGgcgc      <  2:2960910/93‑1 (MQ=255)
                                                                                    aaaaGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCgca     >  1:1650696/1‑93 (MQ=255)
                                                                                     aaaGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCgcag    >  1:102604/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCA  >  NC_000913/3927841‑3928020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAA  >  NC_000913/3927838‑3928024
                                                                                             |                                                                                             
GTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAGCAGCACTGCGACCGCTAAAAAAGGT                                                                                               <  SRR4302535.364397/94‑1 (MQ=60)
GTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGT                                                                                               <  SRR4302535.3716344/94‑1 (MQ=60)
GTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGT                                                                                               <  SRR4302535.4378686/94‑1 (MQ=60)
 TATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTG                                                                                              <  SRR4302535.1956395/94‑1 (MQ=60)
 TATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTG                                                                                              >  SRR4302535.20578/1‑94 (MQ=60)
  ATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGC                                                                                             <  SRR4302535.3709593/94‑1 (MQ=60)
  ATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGC                                                                                             <  SRR4302535.967009/94‑1 (MQ=60)
  ATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGC                                                                                             <  SRR4302535.909180/94‑1 (MQ=60)
  ATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGC                                                                                             >  SRR4302535.4677191/1‑94 (MQ=60)
    CAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTG                                                                                           <  SRR4302535.4390024/94‑1 (MQ=60)
    CAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTG                                                                                           <  SRR4302535.1529334/94‑1 (MQ=60)
    CAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATATACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTG                                                                                           >  SRR4302535.2414351/1‑94 (MQ=60)
     AAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGG                                                                                          <  SRR4302535.4241154/94‑1 (MQ=60)
     AAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGG                                                                                          >  SRR4302535.1920261/1‑94 (MQ=60)
     AAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGG                                                                                          <  SRR4302535.3613544/94‑1 (MQ=60)
     AAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGC                                                                                          >  SRR4302535.720387/1‑94 (MQ=60)
      AATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGC                                                                                         >  SRR4302535.940675/1‑94 (MQ=60)
      AATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGC                                                                                         <  SRR4302535.1694964/94‑1 (MQ=60)
        TTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCT                                                                                       <  SRR4302535.3108066/94‑1 (MQ=60)
         TCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTG                                                                                      <  SRR4302535.3430835/94‑1 (MQ=60)
          CAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGC                                                                                     >  SRR4302535.2421262/1‑94 (MQ=60)
             GCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAAT                                                                                  >  SRR4302535.4678430/1‑94 (MQ=60)
              CTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATA                                                                                 <  SRR4302535.3682305/94‑1 (MQ=60)
               TTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAA                                                                                >  SRR4302535.2812060/1‑94 (MQ=60)
                TTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAAT                                                                               <  SRR4302535.995888/94‑1 (MQ=60)
                TTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAAT                                                                               <  SRR4302535.4444267/94‑1 (MQ=60)
                 TGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATC                                                                              >  SRR4302535.2446398/1‑94 (MQ=60)
                  GCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCG                                                                             >  SRR4302535.4234927/1‑94 (MQ=60)
                  GCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCG                                                                             >  SRR4302535.2780109/1‑94 (MQ=60)
                  GCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCG                                                                             <  SRR4302535.474827/94‑1 (MQ=60)
                   CGCGTCATACCACAGTCAATCTTTCAGCAAAATGAGATCTACCGTGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGG                                                                            <  SRR4302535.2896520/94‑1 (MQ=60)
                   CGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGG                                                                            <  SRR4302535.1642264/94‑1 (MQ=60)
                     CGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACATCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGAT                                                                          <  SRR4302535.2366516/94‑1 (MQ=60)
                     CGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGAT                                                                          >  SRR4302535.4686668/1‑94 (MQ=60)
                     CGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGAT                                                                          >  SRR4302535.1607478/1‑94 (MQ=60)
                      GTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATC                                                                         >  SRR4302535.2212349/1‑94 (MQ=60)
                        CATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGC                                                                       >  SRR4302535.1979509/1‑94 (MQ=60)
                        CATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGC                                                                       >  SRR4302535.2111825/1‑94 (MQ=60)
                        CATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGC                                                                       >  SRR4302535.1673576/1‑94 (MQ=60)
                         ATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCT                                                                      >  SRR4302535.3163218/1‑94 (MQ=60)
                         ATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCT                                                                      >  SRR4302535.2905432/1‑94 (MQ=60)
                         ATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCT                                                                      >  SRR4302535.1928080/1‑94 (MQ=60)
                         ATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCT                                                                      <  SRR4302535.1045510/94‑1 (MQ=60)
                          TACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTT                                                                     >  SRR4302535.4096321/1‑94 (MQ=60)
                          TACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTT                                                                     >  SRR4302535.1039670/1‑94 (MQ=60)
                            CCACAGTCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTT                                                                   <  SRR4302535.2139128/94‑1 (MQ=60)
                            CCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTT                                                                   <  SRR4302535.2311505/94‑1 (MQ=60)
                              ACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTT                                                                 >  SRR4302535.3138548/1‑94 (MQ=60)
                              ACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTT                                                                 <  SRR4302535.3390634/94‑1 (MQ=60)
                              ACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTT                                                                 >  SRR4302535.2172024/1‑94 (MQ=60)
                                AGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCC                                                               >  SRR4302535.180967/1‑94 (MQ=60)
                                  GCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAG                                                             <  SRR4302535.198965/94‑1 (MQ=60)
                                  GCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAG                                                             >  SRR4302535.785583/1‑94 (MQ=60)
                                  GCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAG                                                             >  SRR4302535.2570775/1‑94 (MQ=60)
                                   CAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGC                                                            >  SRR4302535.4603763/1‑94 (MQ=60)
                                   CAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGC                                                            >  SRR4302535.3378399/1‑94 (MQ=60)
                                    AATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCG                                                           >  SRR4302535.30705/1‑94 (MQ=60)
                                    AATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCG                                                           <  SRR4302535.255566/94‑1 (MQ=60)
                                    AATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCG                                                           <  SRR4302535.3220648/94‑1 (MQ=60)
                                      TCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCTACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGAC                                                         <  SRR4302535.3646057/94‑1 (MQ=60)
                                      TCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGAC                                                         <  SRR4302535.1982959/94‑1 (MQ=60)
                                      TCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGAC                                                         <  SRR4302535.2686529/94‑1 (MQ=60)
                                      TCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGAC                                                         <  SRR4302535.1601004/94‑1 (MQ=60)
                                       CTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACG                                                        >  SRR4302535.1837788/1‑94 (MQ=60)
                                       CTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACG                                                        >  SRR4302535.185480/1‑94 (MQ=60)
                                         TTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGAT                                                      <  SRR4302535.2614012/94‑1 (MQ=60)
                                          TCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATC                                                     <  SRR4302535.954068/94‑1 (MQ=60)
                                           CAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCC                                                    <  SRR4302535.685373/94‑1 (MQ=60)
                                            AGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCG                                                   >  SRR4302535.3214266/1‑94 (MQ=60)
                                              CAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAG                                                 >  SRR4302535.2719100/1‑94 (MQ=60)
                                              CAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAG                                                 <  SRR4302535.1347522/94‑1 (MQ=60)
                                                AAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGAC                                               >  SRR4302535.1847293/1‑94 (MQ=60)
                                                AAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGAC                                               <  SRR4302535.2298425/94‑1 (MQ=60)
                                                AAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGAC                                               <  SRR4302535.684049/94‑1 (MQ=60)
                                                AAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGAC                                               >  SRR4302535.1071506/1‑94 (MQ=60)
                                                AAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGAC                                               <  SRR4302535.2564389/94‑1 (MQ=60)
                                                AAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGAC                                               >  SRR4302535.2097591/1‑94 (MQ=60)
                                                AAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGACCCGAGAC                                               >  SRR4302535.3442662/1‑94 (MQ=60)
                                                 AATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACA                                              <  SRR4302535.2149188/94‑1 (MQ=60)
                                                   TGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATA                                            >  SRR4302535.1412038/1‑94 (MQ=60)
                                                    GAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAA                                           <  SRR4302535.1552742/94‑1 (MQ=60)
                                                     CGATCTACAGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAG                                          <  SRR4302535.4531062/94‑1 (MQ=60)
                                                     AGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAG                                          <  SRR4302535.37929/94‑1 (MQ=60)
                                                     AGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAG                                          <  SRR4302535.3107809/94‑1 (MQ=60)
                                                     AGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAG                                          <  SRR4302535.1609896/94‑1 (MQ=60)
                                                     AGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAG                                          <  SRR4302535.1218600/94‑1 (MQ=60)
                                                      GATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGC                                         >  SRR4302535.3014294/1‑94 (MQ=60)
                                                       ATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGC                                        >  SRR4302535.2233592/1‑94 (MQ=60)
                                                       ATCGACCGGGGCAACAGCACTGCGACCGCGAAAATAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGC                                        <  SRR4302535.3875967/94‑1 (MQ=60)
                                                        TCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCG                                       >  SRR4302535.3953885/1‑94 (MQ=60)
                                                         CTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGC                                      >  SRR4302535.2738814/1‑94 (MQ=60)
                                                         CTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGC                                      >  SRR4302535.2341536/1‑94 (MQ=60)
                                                          TACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCA                                     >  SRR4302535.3976076/1‑94 (MQ=60)
                                                           ACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAT                                    <  SRR4302535.3231482/94‑1 (MQ=60)
                                                           ACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAT                                    >  SRR4302535.1584690/1‑94 (MQ=60)
                                                           ACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAT                                    <  SRR4302535.978941/94‑1 (MQ=60)
                                                           ACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAT                                    >  SRR4302535.3920527/1‑94 (MQ=60)
                                                           ACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCAT                                    <  SRR4302535.4127701/94‑1 (MQ=60)
                                                           ACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGACTGCAATAATCGGATCGCGTTTTTCCAGCGACGATCCGAGACACAAGGCGCAT                                    >  SRR4302535.2874915/1‑94 (MQ=60)
                                                            CCGGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATC                                   <  SRR4302535.1289185/94‑1 (MQ=60)
                                                              GGGGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCG                                 >  SRR4302535.2712764/1‑94 (MQ=60)
                                                              GGGGCAACAGCACTGCGACCGCTAAAAAAGGAGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCCTCCG                                 >  SRR4302535.30948/1‑94 (MQ=60)
                                                                GGCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGT                               <  SRR4302535.3486257/94‑1 (MQ=60)
                                                                 GCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTA                              <  SRR4302535.4001889/94‑1 (MQ=60)
                                                                 GCAACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTA                              <  SRR4302535.607773/94‑1 (MQ=60)
                                                                   AACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAAT                            >  SRR4302535.843783/1‑94 (MQ=60)
                                                                    ACAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATT                           <  SRR4302535.2338702/94‑1 (MQ=60)
                                                                     CAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTT                          <  SRR4302535.4034166/94‑1 (MQ=60)
                                                                     CAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTT                          <  SRR4302535.1466154/94‑1 (MQ=60)
                                                                     CAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTT                          >  SRR4302535.425671/1‑94 (MQ=60)
                                                                     CAGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTT                          <  SRR4302535.1984037/94‑1 (MQ=60)
                                                                      AGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTA                         >  SRR4302535.2734031/1‑94 (MQ=60)
                                                                      AGCACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTA                         >  SRR4302535.2613509/1‑94 (MQ=60)
                                                                        CACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATC                       >  SRR4302535.825380/1‑94 (MQ=60)
                                                                        CACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATC                       <  SRR4302535.4497535/94‑1 (MQ=60)
                                                                        CACTGCGACCGCTAAAAAAGGGGCTGGTCTGCAATAATCGGATCGCTTTTTTCCAGCGAGGATCCGAGACATAAGGCGCATCCGGTAATTTATC                       >  SRR4302535.2899498/1‑94 (MQ=60)
                                                                         ACTGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCC                      >  SRR4302535.2865695/1‑94 (MQ=60)
                                                                           TGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAG                    <  SRR4302535.1457111/94‑1 (MQ=60)
                                                                           TGCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAG                    <  SRR4302535.1754460/94‑1 (MQ=60)
                                                                            GCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGT                   >  SRR4302535.4452040/1‑94 (MQ=60)
                                                                            GCGACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGT                   <  SRR4302535.223742/94‑1 (MQ=60)
                                                                              GACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTG                 <  SRR4302535.2826603/94‑1 (MQ=60)
                                                                              GACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTG                 <  SRR4302535.898428/94‑1 (MQ=60)
                                                                              GACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTG                 <  SRR4302535.199403/94‑1 (MQ=60)
                                                                               ACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGC                <  SRR4302535.2874264/94‑1 (MQ=60)
                                                                               ACCGCTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGC                >  SRR4302535.1102486/1‑94 (MQ=60)
                                                                                   CTAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGC            >  SRR4302535.125205/1‑94 (MQ=60)
                                                                                    TAAAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCG           >  SRR4302535.2359183/1‑94 (MQ=60)
                                                                                      AAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCA         >  SRR4302535.1662633/1‑94 (MQ=60)
                                                                                      AAAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCA         >  SRR4302535.552787/1‑94 (MQ=60)
                                                                                       AAAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAG        >  SRR4302535.103332/1‑94 (MQ=60)
                                                                                        AAAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGC       <  SRR4302535.4412278/94‑1 (MQ=60)
                                                                                         AAGGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCA      >  SRR4302535.2240199/1‑94 (MQ=60)
                                                                                           GGTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATA    <  SRR4302535.4380199/94‑1 (MQ=60)
                                                                                            GTGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAA   >  SRR4302535.1697763/1‑94 (MQ=60)
                                                                                             TGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAA  <  SRR4302535.3514490/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTATCAAATTCAGGCTTTGCGCGTCATACCACAGGCAATCTTTCAGCAAAATGAGATCTACCGGGGCAACAGCACTGCGACCGCTAAAAAAGGCGCTGGCCTGCAATAATCGGATCGCTTTTTTCCAGCGACGATCCGAGACATAAGGCGCATCCGGTAATTTATCCAGTTGCTGGCGCAGCATAAA  >  NC_000913/3927838‑3928024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑