Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,962,662 A→G M195T (ATG→ACG)  rhlB ← putative ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,962,6620AG100.0% 603.3 / NA 193M195T (ATG→ACG) rhlBputative ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (82/111);  total (82/111)

Only 100 of 205 total aligned reads displayed.

TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG  >  NC_000913/3962572‑3962751
                                                                                          |                                                                                         
tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGGAAGCGTGGCGGAGAACAGCGTg                                                                                         >  2:3441763/1‑93 (MQ=255)
 gTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCgtgt                                                                                        <  2:3895287/93‑1 (MQ=255)
  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGCGAACAGCGTGtt                                                                                       >  2:2386016/1‑93 (MQ=255)
  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGGACAGCGTGtt                                                                                       >  2:1234059/1‑93 (MQ=255)
  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGCACAGCGTGtt                                                                                       >  1:2597957/1‑93 (MQ=255)
  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGtt                                                                                       <  2:4613373/93‑1 (MQ=255)
   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      >  1:2984966/1‑93 (MQ=255)
   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      >  2:3752363/1‑93 (MQ=255)
    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  1:516960/93‑1 (MQ=255)
    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  1:2689143/93‑1 (MQ=255)
    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  2:1942562/93‑1 (MQ=255)
      ggTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAgg                                                                                   <  2:105098/93‑1 (MQ=255)
        ttCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcg                                                                                 >  1:607591/1‑93 (MQ=255)
        ttCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcg                                                                                 >  2:764090/1‑93 (MQ=255)
         tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcgc                                                                                <  2:2589586/93‑1 (MQ=255)
          ccACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCt                                                                               >  2:3986511/1‑93 (MQ=255)
           cACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTg                                                                              <  2:4125941/93‑1 (MQ=255)
           cACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTg                                                                              <  2:2054324/93‑1 (MQ=255)
           cACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGGGGTGAGGCGCTg                                                                              >  2:4279768/1‑93 (MQ=255)
             cTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGt                                                                            <  1:3434133/93‑1 (MQ=255)
              ttCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGtt                                                                           <  2:58043/93‑1 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       <  2:1614015/93‑1 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       <  2:2527405/93‑1 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       >  2:4181042/1‑93 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      <  1:4430881/93‑1 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  2:3520595/1‑93 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  2:4030630/1‑93 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  1:3020379/1‑93 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGAGCTGGTTTGCAg                                                                      >  1:3207514/1‑93 (MQ=255)
                    atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                     >  1:527710/1‑93 (MQ=255)
                    atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                     <  1:279178/93‑1 (MQ=255)
                     tatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggc                                                                    >  1:4239708/1‑93 (MQ=255)
                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   <  1:3354614/93‑1 (MQ=255)
                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   <  1:1707740/93‑1 (MQ=255)
                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGCGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   >  1:1834462/1‑93 (MQ=255)
                       ttCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTCCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcgg                                                                  <  2:443509/93‑1 (MQ=255)
                         cGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCa                                                                <  1:1322325/93‑1 (MQ=255)
                          ggCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAt                                                               >  1:3722607/1‑93 (MQ=255)
                           gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                              <  1:2899252/93‑1 (MQ=255)
                           gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                              <  1:4290834/93‑1 (MQ=255)
                            cATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAc                                                             <  1:1159833/93‑1 (MQ=255)
                            cATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAc                                                             <  1:2403903/93‑1 (MQ=255)
                             aTTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcg                                                            >  1:2854023/1‑93 (MQ=255)
                              ttGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGGAGGAGGCATAcag                                                           >  2:147145/1‑91 (MQ=255)
                               tGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggc                                                          <  1:3045437/93‑1 (MQ=255)
                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         <  1:448405/93‑1 (MQ=255)
                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         >  2:825044/1‑93 (MQ=255)
                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         >  2:1763305/1‑93 (MQ=255)
                                 ttCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcgg                                                        <  1:3502759/93‑1 (MQ=255)
                                    aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGGAc                                                     >  2:954546/1‑93 (MQ=255)
                                    aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAc                                                     <  2:2850391/93‑1 (MQ=255)
                                    aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAc                                                     >  1:3418825/1‑93 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGCACAGCGCGTTGAGTCGCTGGTTTGCAGGCGGCATTcggcgggagc                                                    >  2:4105132/1‑89 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    >  2:2913684/1‑93 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    >  1:403123/1‑93 (MQ=255)
                                      cTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcag                                                   <  1:1998060/93‑1 (MQ=255)
                                         cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcca                                                <  1:1192460/93‑1 (MQ=255)
                                         cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcca                                                <  1:2018835/93‑1 (MQ=255)
                                          tCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccag                                               <  2:3719375/93‑1 (MQ=255)
                                            gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGGACAGCCAGCg                                             >  2:1826297/1‑93 (MQ=255)
                                            gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCg                                             <  1:3621999/93‑1 (MQ=255)
                                             aaCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCgg                                            <  1:2824994/93‑1 (MQ=255)
                                              aCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGa                                           >  1:3104778/1‑93 (MQ=255)
                                              aCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGa                                           <  1:436937/93‑1 (MQ=255)
                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         <  1:3925019/93‑1 (MQ=255)
                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         <  1:1396546/93‑1 (MQ=255)
                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         <  2:202359/93‑1 (MQ=255)
                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGCGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         >  1:1733556/1‑93 (MQ=255)
                                                 ccAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGatat                                        >  1:461019/1‑93 (MQ=255)
                                                  cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATc                                       <  2:88688/93‑1 (MQ=255)
                                                   aGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCt                                      <  1:711645/93‑1 (MQ=255)
                                                     ttCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCttt                                    <  1:1903558/93‑1 (MQ=255)
                                                      tCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTta                                   <  1:957368/93‑1 (MQ=255)
                                                       cACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaa                                  <  1:1457045/93‑1 (MQ=255)
                                                       cACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaa                                  <  2:159280/93‑1 (MQ=255)
                                                        aCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaat                                 >  2:4340000/1‑93 (MQ=255)
                                                         cGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaata                                <  1:2232054/93‑1 (MQ=255)
                                                         cGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaata                                <  1:882530/93‑1 (MQ=255)
                                                           tACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATaaa                              <  2:1554433/93‑1 (MQ=255)
                                                            aCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAg                             >  2:3947318/1‑93 (MQ=255)
                                                              ccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATaaaaac                           >  2:2754997/1‑90 (MQ=255)
                                                              ccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                           <  1:680420/93‑1 (MQ=255)
                                                               cGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGccc                          <  1:1797067/93‑1 (MQ=255)
                                                                ggTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCa                         <  2:2999711/93‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  1:3468554/93‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  2:1896992/93‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  1:3509528/93‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  1:4674/93‑1 (MQ=255)
                                                                      aaaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGt                   >  1:214156/1‑93 (MQ=255)
                                                                      aaaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGt                   >  1:2273497/1‑93 (MQ=255)
                                                                      aaaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGt                   >  1:759355/1‑93 (MQ=255)
                                                                          cGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt               <  2:2456504/93‑1 (MQ=255)
                                                                          cGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt               <  2:1420616/93‑1 (MQ=255)
                                                                           gTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATg              <  1:999436/93‑1 (MQ=255)
                                                                                ggAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATc         <  2:1644823/93‑1 (MQ=255)
                                                                                 gagaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCg        >  1:2214421/1‑93 (MQ=255)
                                                                                   gaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGc      >  2:331702/1‑93 (MQ=255)
                                                                                    aaCAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCt     >  1:3810936/1‑93 (MQ=255)
                                                                                     aCAGCGGGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCtt    <  1:3074323/93‑1 (MQ=255)
                                                                                       aGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg  >  1:2693651/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG  >  NC_000913/3962572‑3962751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCA  >  NC_000913/3962569‑3962755
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TTCTGTTCCGGATCCACTCGAATATATCCGGCATTGTTCATCTGCTGGAAAGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCG                                                                                               <  SRR4302535.2955912/94‑1 (MQ=60)
 TCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGT                                                                                              <  SRR4302535.830546/94‑1 (MQ=60)
 TCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGT                                                                                              <  SRR4302535.4287367/94‑1 (MQ=60)
  CTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTG                                                                                             <  SRR4302535.207100/94‑1 (MQ=60)
  CTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTG                                                                                             <  SRR4302535.211575/94‑1 (MQ=60)
    GTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGCACAGCGTGTT                                                                                           >  SRR4302535.2616617/1‑94 (MQ=60)
    GTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTT                                                                                           >  SRR4302535.592584/1‑94 (MQ=60)
    GTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTT                                                                                           <  SRR4302535.2884696/94‑1 (MQ=60)
     TTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTG                                                                                          >  SRR4302535.3005903/1‑94 (MQ=60)
       CCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAG                                                                                        <  SRR4302535.2708362/94‑1 (MQ=60)
       CCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAG                                                                                        <  SRR4302535.520577/94‑1 (MQ=60)
       CCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAG                                                                                        <  SRR4302535.4225585/94‑1 (MQ=60)
        CGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGG                                                                                       <  SRR4302535.1944583/94‑1 (MQ=60)
          GTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCG                                                                                     >  SRR4302535.2476023/1‑94 (MQ=60)
          GTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCG                                                                                     >  SRR4302535.611832/1‑94 (MQ=60)
             CCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTG                                                                                  >  SRR4302535.2133464/1‑94 (MQ=60)
             CCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTG                                                                                  >  SRR4302535.2839783/1‑94 (MQ=60)
                CTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTT                                                                               <  SRR4302535.3458000/94‑1 (MQ=60)
                 TTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGGTT                                                                              >  SRR4302535.4402597/1‑94 (MQ=60)
                   CAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGC                                                                            >  SRR4302535.2286457/1‑94 (MQ=60)
                    AATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGCACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCA                                                                           >  SRR4302535.309/1‑94 (MQ=60)
                     ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAG                                                                          <  SRR4302535.3104055/94‑1 (MQ=60)
                     ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAG                                                                          >  SRR4302535.3041531/1‑94 (MQ=60)
                     ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAG                                                                          >  SRR4302535.2756253/1‑94 (MQ=60)
                     ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAG                                                                          >  SRR4302535.4043250/1‑94 (MQ=60)
                     ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGAGCTGGTTTGCAG                                                                          >  SRR4302535.3229730/1‑94 (MQ=60)
                      TATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGG                                                                         >  SRR4302535.531395/1‑94 (MQ=60)
                      TATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGG                                                                         <  SRR4302535.2668322/94‑1 (MQ=60)
                      TATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGG                                                                         <  SRR4302535.4461241/94‑1 (MQ=60)
                       ATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGC                                                                        <  SRR4302535.281125/94‑1 (MQ=60)
                       ATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGC                                                                        >  SRR4302535.4268729/1‑94 (MQ=60)
                        TATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGTGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCG                                                                       <  SRR4302535.3514100/94‑1 (MQ=60)
                        TATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCG                                                                       >  SRR4302535.2242221/1‑94 (MQ=60)
                        TATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGCGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCG                                                                       >  SRR4302535.1847699/1‑94 (MQ=60)
                         ATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGG                                                                      <  SRR4302535.1720086/94‑1 (MQ=60)
                         ATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGG                                                                      >  SRR4302535.3427973/1‑94 (MQ=60)
                         ATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGG                                                                      <  SRR4302535.3377973/94‑1 (MQ=60)
                         ATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGG                                                                      >  SRR4302535.2323326/1‑94 (MQ=60)
                            CGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCCGGCGGCAT                                                                   >  SRR4302535.703261/1‑94 (MQ=60)
                            CGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAT                                                                   >  SRR4302535.3748394/1‑94 (MQ=60)
                            CGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAT                                                                   <  SRR4302535.1331763/94‑1 (MQ=60)
                              GCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAC                                                                 <  SRR4302535.2919751/94‑1 (MQ=60)
                              GCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAC                                                                 <  SRR4302535.4320192/94‑1 (MQ=60)
                               CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                <  SRR4302535.2421197/94‑1 (MQ=60)
                               CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                >  SRR4302535.3455571/1‑94 (MQ=60)
                               CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                <  SRR4302535.1168122/94‑1 (MQ=60)
                               CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                <  SRR4302535.1232806/94‑1 (MQ=60)
                               CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                >  SRR4302535.2874239/1‑94 (MQ=60)
                                ATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCGTACGG                                                               <  SRR4302535.2712689/94‑1 (MQ=60)
                                ATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGG                                                               >  SRR4302535.800708/1‑94 (MQ=60)
                                 TTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGC                                                              >  SRR4302535.3937601/1‑94 (MQ=60)
                                  TGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCG                                                             >  SRR4302535.1811792/1‑94 (MQ=60)
                                  TGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCG                                                             <  SRR4302535.3066771/94‑1 (MQ=60)
                                   GTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGG                                                            <  SRR4302535.451547/94‑1 (MQ=60)
                                   GTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGG                                                            <  SRR4302535.3753359/94‑1 (MQ=60)
                                   GTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGG                                                            <  SRR4302535.4716604/94‑1 (MQ=60)
                                    TTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGA                                                           <  SRR4302535.3527105/94‑1 (MQ=60)
                                      CATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAC                                                         >  SRR4302535.3442605/1‑94 (MQ=60)
                                       ATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACA                                                        >  SRR4302535.405947/1‑94 (MQ=60)
                                        TCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAG                                                       <  SRR4302535.641090/94‑1 (MQ=60)
                                         CTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGC                                                      <  SRR4302535.2012450/94‑1 (MQ=60)
                                         CTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGC                                                      >  SRR4302535.1802179/1‑94 (MQ=60)
                                          TGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCC                                                     <  SRR4302535.329547/94‑1 (MQ=60)
                                            CTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAG                                                   <  SRR4302535.2033385/94‑1 (MQ=60)
                                            CTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAG                                                   <  SRR4302535.1201000/94‑1 (MQ=60)
                                             TCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGC                                                  <  SRR4302535.1191349/94‑1 (MQ=60)
                                              CGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCG                                                 >  SRR4302535.4302660/1‑94 (MQ=60)
                                               GAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACcacgcagatcggaagag                                                >  SRR4302535.2775161/1‑77 (MQ=60)
                                               GAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGG                                                <  SRR4302535.3647107/94‑1 (MQ=60)
                                                AACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGA                                               <  SRR4302535.2845048/94‑1 (MQ=60)
                                                AACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGA                                               >  SRR4302535.3126395/1‑94 (MQ=60)
                                                AACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGA                                               <  SRR4302535.2221768/94‑1 (MQ=60)
                                                 ACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGAT                                              <  SRR4302535.439988/94‑1 (MQ=60)
                                                 ACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGAT                                              <  SRR4302535.892542/94‑1 (MQ=60)
                                                 ACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGAT                                              <  SRR4302535.747334/94‑1 (MQ=60)
                                                 ACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGAT                                              <  SRR4302535.4051507/94‑1 (MQ=60)
                                                  CGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATA                                             >  SRR4302535.3459931/1‑94 (MQ=60)
                                                  CGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGCGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATA                                             >  SRR4302535.1746086/1‑94 (MQ=60)
                                                   GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            <  SRR4302535.1000156/94‑1 (MQ=60)
                                                   GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            <  SRR4302535.4327110/94‑1 (MQ=60)
                                                   GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            <  SRR4302535.1406519/94‑1 (MQ=60)
                                                   GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            <  SRR4302535.3951839/94‑1 (MQ=60)
                                                   GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            >  SRR4302535.3210576/1‑94 (MQ=60)
                                                   GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            <  SRR4302535.3546831/94‑1 (MQ=60)
                                                   GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            >  SRR4302535.464241/1‑94 (MQ=60)
                                                    CCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATC                                           >  SRR4302535.3723499/1‑94 (MQ=60)
                                                     CAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCT                                          <  SRR4302535.1733149/94‑1 (MQ=60)
                                                     CAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCT                                          <  SRR4302535.1683334/94‑1 (MQ=60)
                                                      AGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTT                                         <  SRR4302535.716641/94‑1 (MQ=60)
                                                        TTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTA                                       <  SRR4302535.1917258/94‑1 (MQ=60)
                                                         TCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAA                                      <  SRR4302535.964166/94‑1 (MQ=60)
                                                          CACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAAT                                     <  SRR4302535.1467471/94‑1 (MQ=60)
                                                            CGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAA                                   <  SRR4302535.888779/94‑1 (MQ=60)
                                                            CGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAA                                   <  SRR4302535.2247926/94‑1 (MQ=60)
                                                             GTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAA                                  <  SRR4302535.4479641/94‑1 (MQ=60)
                                                                CCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCCGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCC                               >  SRR4302535.2857953/1‑94 (MQ=60)
                                                                CCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCC                               <  SRR4302535.2960573/94‑1 (MQ=60)
                                                                 CCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCC                              <  SRR4302535.685186/94‑1 (MQ=60)
                                                                 CCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCC                              <  SRR4302535.4655956/94‑1 (MQ=60)
                                                                  CGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCA                             >  SRR4302535.4551606/1‑94 (MQ=60)
                                                                  CGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCA                             <  SRR4302535.1810059/94‑1 (MQ=60)
                                                                    GTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAACACCAGA                           >  SRR4302535.3015455/1‑94 (MQ=60)
                                                                      ACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATC                         <  SRR4302535.3533917/94‑1 (MQ=60)
                                                                      ACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATC                         <  SRR4302535.3492656/94‑1 (MQ=60)
                                                                      ACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATC                         <  SRR4302535.4717/94‑1 (MQ=60)
                                                                       CGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCG                        >  SRR4302535.3066922/1‑94 (MQ=60)
                                                                        GAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGT                       >  SRR4302535.764707/1‑94 (MQ=60)
                                                                        GAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGT                       >  SRR4302535.2289719/1‑94 (MQ=60)
                                                                        GAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGT                       >  SRR4302535.215622/1‑94 (MQ=60)
                                                                           AGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACA                    <  SRR4302535.2030574/94‑1 (MQ=60)
                                                                           AGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACA                    <  SRR4302535.1859395/94‑1 (MQ=60)
                                                                            GCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAT                   >  SRR4302535.1384239/1‑94 (MQ=60)
                                                                             CGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATG                  <  SRR4302535.3532687/94‑1 (MQ=60)
                                                                             CGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATG                  <  SRR4302535.1424921/94‑1 (MQ=60)
                                                                              GTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGC                 <  SRR4302535.1006523/94‑1 (MQ=60)
                                                                                   GGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCG            >  SRR4302535.2230189/1‑94 (MQ=60)
                                                                                     AGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGC          >  SRR4302535.52953/1‑94 (MQ=60)
                                                                                      GAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCT         >  SRR4302535.3837231/1‑94 (MQ=60)
                                                                                        ACAGCGGGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTC       <  SRR4302535.3095778/94‑1 (MQ=60)
                                                                                         CAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG      >  SRR4302535.2712900/1‑94 (MQ=60)
                                                                                          AGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGT     >  SRR4302535.1277993/1‑94 (MQ=60)
                                                                                           GCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTC    <  SRR4302535.1341381/94‑1 (MQ=60)
                                                                                            CGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCC   >  SRR4302535.506542/1‑94 (MQ=60)
                                                                                             GTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCA  <  SRR4302535.4107531/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCA  >  NC_000913/3962569‑3962755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑