Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,820 C→T R317C (CGC→TGC)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,8200CT99.6% 863.5 / NA 241R317C (CGC→TGC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (97/143);  total (98/143)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 246 total aligned reads displayed.

ACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCT  >  NC_000913/3969730‑3969909
                                                                                          |                                                                                         
aCCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAAtgc                                                                                         <  1:2221220/93‑1 (MQ=255)
aCCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAAtgc                                                                                         >  2:2697625/1‑93 (MQ=255)
aCCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAAtgc                                                                                         <  2:3856289/93‑1 (MQ=255)
  cgcgcgcgAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATgcgc                                                                                       >  2:856638/1‑93 (MQ=255)
   gcgcgcgAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGcc                                                                                      <  2:2914278/93‑1 (MQ=255)
       gcgAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTCCCGATAAATGCGCCAGTg                                                                                  >  2:2927056/1‑93 (MQ=255)
          aaGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAAc                                                                               <  1:627904/93‑1 (MQ=255)
          aaGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAAc                                                                               <  1:1530129/93‑1 (MQ=255)
          aaGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAAc                                                                               <  2:2814679/93‑1 (MQ=255)
          aaGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAAc                                                                               <  2:2189635/93‑1 (MQ=255)
           aGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACt                                                                              <  2:4596443/93‑1 (MQ=255)
           aGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACt                                                                              <  1:2440149/93‑1 (MQ=255)
                aCGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGaaaa                                                                         <  1:2912191/93‑1 (MQ=255)
                aCGATCACAAACCGTTCTAGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGaaaa                                                                         <  2:3120952/93‑1 (MQ=255)
                 cGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAAt                                                                        <  1:77840/93‑1 (MQ=255)
                  gATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATc                                                                       <  2:3342958/93‑1 (MQ=255)
                   aTCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCg                                                                      >  1:2924372/1‑93 (MQ=255)
                   aTCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCg                                                                      >  1:3820314/1‑93 (MQ=255)
                     cacaACCCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGcc                                                                    <  1:70997/93‑1 (MQ=255)
                     cacaAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGcc                                                                    <  2:1209341/93‑1 (MQ=255)
                     cacaAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGcc                                                                    <  1:469606/93‑1 (MQ=255)
                      acaAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCt                                                                   <  1:4603710/93‑1 (MQ=255)
                       caAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTg                                                                  >  2:3184470/1‑93 (MQ=255)
                       caAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTg                                                                  >  1:2108272/1‑93 (MQ=255)
                         aaCCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCt                                                                >  2:1357532/1‑93 (MQ=255)
                          aCCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCtt                                                               >  1:4090493/1‑93 (MQ=255)
                          aCCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCtt                                                               <  2:2457136/93‑1 (MQ=255)
                              ttCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGt                                                           >  1:16669/1‑93 (MQ=255)
                              ttCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGt                                                           >  2:667666/1‑93 (MQ=255)
                               tCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTc                                                          <  1:1976766/93‑1 (MQ=255)
                                  gggTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTgg                                                       <  1:2742902/93‑1 (MQ=255)
                                  gggTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTgg                                                       >  2:201532/1‑93 (MQ=255)
                                   ggTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGc                                                      >  2:2987737/1‑93 (MQ=255)
                                   ggTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGc                                                      >  2:3735886/1‑93 (MQ=255)
                                   ggTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGc                                                      <  1:4528444/93‑1 (MQ=255)
                                   ggTTATCGATCAGGTGAAACCGGCGTTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGc                                                      <  2:978708/93‑1 (MQ=255)
                                     ttATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGt                                                    <  2:1407376/93‑1 (MQ=255)
                                     ttATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGt                                                    <  2:4124965/93‑1 (MQ=255)
                                       aTCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGttt                                                  >  1:3871214/1‑93 (MQ=255)
                                          gATCAGGTGAAGGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTaaa                                               <  2:1886264/93‑1 (MQ=255)
                                           aTCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAAc                                              <  1:948408/93‑1 (MQ=255)
                                           aTCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAAc                                              >  2:3387229/1‑93 (MQ=255)
                                               ggTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGaa                                          <  2:1099249/93‑1 (MQ=255)
                                               ggTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGaa                                          <  2:758316/93‑1 (MQ=255)
                                               ggTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGaa                                          >  1:2785787/1‑93 (MQ=255)
                                                gTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAat                                         <  2:1150650/93‑1 (MQ=255)
                                                 tGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAata                                        <  1:3129722/93‑1 (MQ=255)
                                                 tGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAata                                        <  1:412098/93‑1 (MQ=255)
                                                 tGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTCGGTCTGGCGTTTAAACCGAata                                        <  2:615837/93‑1 (MQ=255)
                                                  gAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAatat                                       <  1:2717780/93‑1 (MQ=255)
                                                  gAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAatat                                       <  1:1917827/93‑1 (MQ=255)
                                                   aaaGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATAtt                                      <  1:4610288/93‑1 (MQ=255)
                                                   aaaGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATAtt                                      <  2:2953847/93‑1 (MQ=255)
                                                   aaaGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATAtt                                      <  1:2310246/93‑1 (MQ=255)
                                                    aaGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATtg                                     <  1:4457064/93‑1 (MQ=255)
                                                        ggcggTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATtgatga                                 <  2:2183706/93‑1 (MQ=255)
                                                         gcggTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGAc                                >  1:3449649/1‑93 (MQ=255)
                                                          cggTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGAcc                               <  1:365013/93‑1 (MQ=255)
                                                          cggTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGAcc                               <  2:4386669/93‑1 (MQ=255)
                                                          cggTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGAcc                               >  1:1636786/1‑93 (MQ=255)
                                                          cggTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGAcc                               <  2:3218360/93‑1 (MQ=255)
                                                          cggTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGAcc                               <  2:8836/93‑1 (MQ=255)
                                                            gcggCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTg                             <  1:1014866/91‑1 (MQ=255)
                                                             tGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTgc                            <  2:1614877/93‑1 (MQ=255)
                                                             tGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTgc                            <  1:2870968/93‑1 (MQ=255)
                                                                 tGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGa                        <  2:2822545/93‑1 (MQ=255)
                                                                  gATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGaa                       >  1:529250/1‑93 (MQ=255)
                                                                  gATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGaa                       >  2:57253/1‑93 (MQ=255)
                                                                  gATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGaa                       >  1:1512568/1‑93 (MQ=255)
                                                                  gATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGaa                       <  2:3259233/93‑1 (MQ=255)
                                                                  gATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGaa                       >  1:224860/1‑93 (MQ=255)
                                                                  gATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGaa                       >  1:4502306/1‑93 (MQ=255)
                                                                  gATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGaa                       <  2:2377375/93‑1 (MQ=255)
                                                                    ttGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAg                     <  1:1332698/93‑1 (MQ=255)
                                                                     tGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGc                    >  1:1772511/1‑93 (MQ=255)
                                                                      gCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGcc                   <  1:2486884/93‑1 (MQ=255)
                                                                       ccTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGccc                  <  1:2121618/93‑1 (MQ=255)
                                                                       ccTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGccc                  <  1:1739878/93‑1 (MQ=255)
                                                                        cTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCg                 >  2:1355720/1‑93 (MQ=255)
                                                                        cTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCg                 >  1:3710056/1‑93 (MQ=255)
                                                                         tGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCgg                >  2:4465888/1‑93 (MQ=255)
                                                                          ggcggcTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGc               >  2:841377/1‑93 (MQ=255)
                                                                            cggcTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTTAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGa             <  1:3229260/93‑1 (MQ=255)
                                                                            cggcTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGa             <  2:2634396/93‑1 (MQ=255)
                                                                             ggcTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGAt            <  2:4319642/93‑1 (MQ=255)
                                                                              gcTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGCAAGCCCGGCGATg           >  2:4245647/1‑93 (MQ=255)
                                                                              gcTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATg           <  2:1482909/93‑1 (MQ=255)
                                                                              gcTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATg           >  2:3497085/1‑93 (MQ=255)
                                                                               cTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATgg          <  1:1175205/93‑1 (MQ=255)
                                                                                  ccGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGaaa       <  2:2255774/93‑1 (MQ=255)
                                                                                   cGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAAt      <  2:482514/93‑1 (MQ=255)
                                                                                    gATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATc     >  1:2101109/1‑93 (MQ=255)
                                                                                    gATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATc     >  1:4252717/1‑93 (MQ=255)
                                                                                    gATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATc     <  2:3148386/93‑1 (MQ=255)
                                                                                     aTAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCg    >  2:3931453/1‑93 (MQ=255)
                                                                                     aTAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCg    >  1:4043822/1‑93 (MQ=255)
                                                                                     aTAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCg    >  1:2559755/1‑93 (MQ=255)
                                                                                      tAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGc   >  2:183552/1‑93 (MQ=255)
                                                                                      tAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGc   <  1:3345517/93‑1 (MQ=255)
                                                                                       aaaTGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCt  <  1:2332737/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCT  >  NC_000913/3969730‑3969909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTGAAC  >  NC_000913/3969727‑3969913
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CGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAAT                                                                                               >  SRR4302535.4050267/1‑94 (MQ=60)
CGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAAT                                                                                               <  SRR4302535.3302220/94‑1 (MQ=60)
CGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGCCTGATTGCCTGGCGGCTACCGATAAAT                                                                                               >  SRR4302535.1486100/1‑94 (MQ=60)
 GTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATG                                                                                              <  SRR4302535.3739593/94‑1 (MQ=60)
 GTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATG                                                                                              <  SRR4302535.4325983/94‑1 (MQ=60)
   ACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCG                                                                                            <  SRR4302535.2237035/94‑1 (MQ=60)
       CGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAG                                                                                        <  SRR4302535.1882387/94‑1 (MQ=60)
         GGCGAAGTGAGCGATCACAAACCTTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTG                                                                                      <  SRR4302535.2326178/94‑1 (MQ=60)
         CGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTG                                                                                      <  SRR4302535.3761707/94‑1 (MQ=60)
          GCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGA                                                                                     <  SRR4302535.33482/94‑1 (MQ=60)
           CGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAA                                                                                    >  SRR4302535.224803/1‑94 (MQ=60)
             AAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACT                                                                                  <  SRR4302535.2114711/94‑1 (MQ=60)
             AAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACT                                                                                  <  SRR4302535.1541152/94‑1 (MQ=60)
             AAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACT                                                                                  <  SRR4302535.632278/94‑1 (MQ=60)
              AGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTG                                                                                 <  SRR4302535.2457726/94‑1 (MQ=60)
              AGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTG                                                                                 >  SRR4302535.2523186/1‑94 (MQ=60)
              AGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTG                                                                                 <  SRR4302535.3768939/94‑1 (MQ=60)
               GTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGA                                                                                >  SRR4302535.4123819/1‑94 (MQ=60)
                TGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAA                                                                               <  SRR4302535.2811323/94‑1 (MQ=60)
                  AACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAA                                                                             >  SRR4302535.1569193/1‑94 (MQ=60)
                  AACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAA                                                                             >  SRR4302535.3236638/1‑94 (MQ=60)
                  AACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAA                                                                             >  SRR4302535.4118539/1‑94 (MQ=60)
                   ACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAAT                                                                            <  SRR4302535.1715925/94‑1 (MQ=60)
                   ACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAAT                                                                            <  SRR4302535.4530466/94‑1 (MQ=60)
                   ACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAAT                                                                            <  SRR4302535.2932765/94‑1 (MQ=60)
                    CGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATC                                                                           >  SRR4302535.3474218/1‑94 (MQ=60)
                    CGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATC                                                                           <  SRR4302535.3142434/94‑1 (MQ=60)
                    CGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATC                                                                           <  SRR4302535.78396/94‑1 (MQ=60)
                     GATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCG                                                                          >  SRR4302535.2944992/1‑94 (MQ=60)
                     GATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCG                                                                          >  SRR4302535.3846673/1‑94 (MQ=60)
                     GATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCG                                                                          >  SRR4302535.581957/1‑94 (MQ=60)
                      ATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGC                                                                         >  SRR4302535.1128262/1‑94 (MQ=60)
                       TCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCC                                                                        <  SRR4302535.2419646/94‑1 (MQ=60)
                        CACAACCCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCT                                                                       <  SRR4302535.71518/94‑1 (MQ=60)
                        CACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCT                                                                       <  SRR4302535.4186637/94‑1 (MQ=60)
                        CACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCT                                                                       <  SRR4302535.1697758/94‑1 (MQ=60)
                        CACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCT                                                                       <  SRR4302535.472891/94‑1 (MQ=60)
                        CACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCT                                                                       <  SRR4302535.774933/94‑1 (MQ=60)
                        CACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCT                                                                       <  SRR4302535.2521739/94‑1 (MQ=60)
                         ACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTG                                                                      >  SRR4302535.2123478/1‑94 (MQ=60)
                         ACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTG                                                                      >  SRR4302535.2123477/1‑94 (MQ=60)
                         ACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTG                                                                      <  SRR4302535.4635010/94‑1 (MQ=60)
                          CAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGC                                                                     <  SRR4302535.2212731/94‑1 (MQ=60)
                            AACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTT                                                                   >  SRR4302535.1880626/1‑94 (MQ=60)
                            AACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTT                                                                   >  SRR4302535.4118482/1‑94 (MQ=60)
                                GTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGT                                                               >  SRR4302535.16795/1‑94 (MQ=60)
                                  TCTGTGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCT                                                             <  SRR4302535.4615410/94‑1 (MQ=60)
                                  TCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCT                                                             <  SRR4302535.1991003/94‑1 (MQ=60)
                                   CTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTG                                                            >  SRR4302535.2583070/1‑94 (MQ=60)
                                    TGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGG                                                           >  SRR4302535.4314170/1‑94 (MQ=60)
                                    TGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGG                                                           >  SRR4302535.1973442/1‑94 (MQ=60)
                                    TGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGG                                                           <  SRR4302535.1615887/94‑1 (MQ=60)
                                     GGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGC                                                          <  SRR4302535.2762453/94‑1 (MQ=60)
                                      GGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCG                                                         <  SRR4302535.4707579/94‑1 (MQ=60)
                                      GGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCG                                                         <  SRR4302535.4559454/94‑1 (MQ=60)
                                        TTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTT                                                       >  SRR4302535.2745621/1‑94 (MQ=60)
                                        TTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTT                                                       >  SRR4302535.2469320/1‑94 (MQ=60)
                                         TATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTT                                                      <  SRR4302535.2800764/94‑1 (MQ=60)
                                         TATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTT                                                      >  SRR4302535.3897817/1‑94 (MQ=60)
                                             GATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAAC                                                  >  SRR4302535.2937400/1‑94 (MQ=60)
                                              ATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACC                                                 <  SRR4302535.955128/94‑1 (MQ=60)
                                                CAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGA                                               >  SRR4302535.4592931/1‑94 (MQ=60)
                                                CAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGA                                               >  SRR4302535.3868478/1‑94 (MQ=60)
                                                 AGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAA                                              >  SRR4302535.2805613/1‑94 (MQ=60)
                                                 AGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAA                                              <  SRR4302535.3726596/94‑1 (MQ=60)
                                                  GGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAAT                                             <  SRR4302535.4509336/94‑1 (MQ=60)
                                                    TGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATAT                                           <  SRR4302535.414995/94‑1 (MQ=60)
                                                    TGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATAT                                           <  SRR4302535.2146475/94‑1 (MQ=60)
                                                    TGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATAT                                           <  SRR4302535.3316466/94‑1 (MQ=60)
                                                    TGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATAT                                           <  SRR4302535.3151519/94‑1 (MQ=60)
                                                     GAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATT                                          <  SRR4302535.2737190/94‑1 (MQ=60)
                                                     GAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATT                                          >  SRR4302535.528857/1‑94 (MQ=60)
                                                     GAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATT                                          <  SRR4302535.230803/94‑1 (MQ=60)
                                                     GAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATT                                          <  SRR4302535.1931647/94‑1 (MQ=60)
                                                      AAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTG                                         <  SRR4302535.4641611/94‑1 (MQ=60)
                                                      AAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTG                                         >  SRR4302535.2077862/1‑94 (MQ=60)
                                                      AAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTG                                         <  SRR4302535.2326782/94‑1 (MQ=60)
                                                       AAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGA                                        <  SRR4302535.4487630/94‑1 (MQ=60)
                                                        AGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGAT                                       <  SRR4302535.1540288/94‑1 (MQ=60)
                                                          CGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGA                                     <  SRR4302535.2757572/94‑1 (MQ=60)
                                                          CGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGA                                     <  SRR4302535.652832/94‑1 (MQ=60)
                                                          CCGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGA                                     <  SRR4302535.782241/94‑1 (MQ=60)
                                                           GGCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGAC                                    >  SRR4302535.3473631/1‑94 (MQ=60)
                                                            GCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACC                                   >  SRR4302535.535526/1‑94 (MQ=60)
                                                            GCGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACC                                   >  SRR4302535.1648609/1‑94 (MQ=60)
                                                             CGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCT                                  <  SRR4302535.3976934/94‑1 (MQ=60)
                                                             CGGTGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCT                                  <  SRR4302535.367524/94‑1 (MQ=60)
                                                               GCGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGC                                <  SRR4302535.1022081/94‑1 (MQ=60)
                                                                TGGCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCG                               <  SRR4302535.2891309/94‑1 (MQ=60)
                                                                  GCTGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCG                             >  SRR4302535.2436753/1‑94 (MQ=60)
                                                                    TGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAA                           >  SRR4302535.532953/1‑94 (MQ=60)
                                                                    TGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAA                           >  SRR4302535.226409/1‑94 (MQ=60)
                                                                    TGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAA                           >  SRR4302535.4533223/1‑94 (MQ=60)
                                                                    TGATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAA                           >  SRR4302535.1523463/1‑94 (MQ=60)
                                                                      gaTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAG                         <  SRR4302535.4533399/92‑1 (MQ=60)
                                                                      ATTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAG                         >  SRR4302535.911259/1‑94 (MQ=60)
                                                                       TTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGC                        <  SRR4302535.4641142/94‑1 (MQ=60)
                                                                       TTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGC                        >  SRR4302535.1785356/1‑94 (MQ=60)
                                                                       TTGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGC                        <  SRR4302535.1342203/94‑1 (MQ=60)
                                                                        TGCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCC                       <  SRR4302535.1427917/94‑1 (MQ=60)
                                                                         GCCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCC                      <  SRR4302535.2504816/94‑1 (MQ=60)
                                                                          GCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCG                     <  SRR4302535.2337450/94‑1 (MQ=60)
                                                                          CCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCG                     <  SRR4302535.2136916/94‑1 (MQ=60)
                                                                          CCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCG                     >  SRR4302535.1331371/1‑94 (MQ=60)
                                                                          CCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCG                     <  SRR4302535.1752451/94‑1 (MQ=60)
                                                                          CCTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCG                     >  SRR4302535.3735750/1‑94 (MQ=60)
                                                                           CTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGG                    <  SRR4302535.1766678/94‑1 (MQ=60)
                                                                           CTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGG                    <  SRR4302535.3985544/94‑1 (MQ=60)
                                                                           CTGGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGG                    <  SRR4302535.3537424/94‑1 (MQ=60)
                                                                             GGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCG                  <  SRR4302535.987198/94‑1 (MQ=60)
                                                                             GGCGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCG                  <  SRR4302535.2864701/94‑1 (MQ=60)
                                                                               CGGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTTAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGAT                <  SRR4302535.3251644/94‑1 (MQ=60)
                                                                                GGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATG               <  SRR4302535.1655867/94‑1 (MQ=60)
                                                                                GGCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATG               <  SRR4302535.4110393/94‑1 (MQ=60)
                                                                                 GCTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGcggttga              >  SRR4302535.4589441/1‑87 (MQ=60)
                                                                                  CTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGA             >  SRR4302535.3415213/1‑94 (MQ=60)
                                                                                  CTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGA             <  SRR4302535.1183594/94‑1 (MQ=60)
                                                                                  CTACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGA             >  SRR4302535.2922650/1‑94 (MQ=60)
                                                                                   TACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAA            <  SRR4302535.2118310/94‑1 (MQ=60)
                                                                                    ACCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAA           <  SRR4302535.4670848/94‑1 (MQ=60)
                                                                                     CCGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAAT          <  SRR4302535.3136020/94‑1 (MQ=60)
                                                                                      CGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATC         >  SRR4302535.4281856/1‑94 (MQ=60)
                                                                                      CGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATC         >  SRR4302535.2116261/1‑94 (MQ=60)
                                                                                      CGATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATC         >  SRR4302535.1468251/1‑94 (MQ=60)
                                                                                       GATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCG        >  SRR4302535.4071507/1‑94 (MQ=60)
                                                                                       GATAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCG        >  SRR4302535.2578183/1‑94 (MQ=60)
                                                                                         TAAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCT      <  SRR4302535.3368821/94‑1 (MQ=60)
                                                                                          AAATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTG     <  SRR4302535.2349428/94‑1 (MQ=60)
                                                                                           AATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTGA    >  SRR4302535.2466692/1‑94 (MQ=60)
                                                                                            ATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTGAA   <  SRR4302535.1979318/94‑1 (MQ=60)
                                                                                            ATGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTGAA   <  SRR4302535.843074/94‑1 (MQ=60)
                                                                                             TGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTGAAC  >  SRR4302535.3204663/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGTACCGCGCGCGAAGTGAACGATCACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTGGCTGATTGCCTGGCGGCTACCGATAAACGCGCCAGTGAACTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTGAAC  >  NC_000913/3969727‑3969913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑