Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,525 +G intergenic (+92/‑141) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,5241.G99.6% 717.8 / ‑4.0 223intergenic (+91/‑142)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (97/125);  total (97/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 241 total aligned reads displayed.

GGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008435‑4008611
                                                                                            |                                                                                       
gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                                                        >  2:4670876/1‑93 (MQ=255)
 ggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                       >  1:1064987/1‑92 (MQ=255)
   aTGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                     <  2:439256/93‑4 (MQ=255)
   aTGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                     <  1:2648648/93‑4 (MQ=255)
    tGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                                                    <  2:3992017/93‑5 (MQ=255)
    tGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                                                    <  1:3060309/93‑5 (MQ=255)
      aaCGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                                                   <  1:3223860/93‑1 (MQ=255)
       acgacgCCGAGATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                                                  <  1:451557/93‑1 (MQ=255)
       acgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                                                  <  1:1118114/93‑1 (MQ=255)
         gacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                                                                >  2:2223405/1‑93 (MQ=255)
         gacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                                                                <  1:4441255/93‑1 (MQ=255)
         gacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                                                                >  2:4158913/1‑93 (MQ=255)
              cGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                                                           >  1:4173572/1‑93 (MQ=255)
               gAAATGCTGGCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAACTTCATCCCGAGCTGGAAGGGATTGGTACt                                                                          >  1:2236980/1‑93 (MQ=255)
                 aaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                                                        <  2:2085270/93‑1 (MQ=255)
                      tGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                                                   >  1:1690261/1‑93 (MQ=255)
                       gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGTAGTGATTGGTACTAATGCcga                                                                  >  1:920091/1‑93 (MQ=255)
                       gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                                                  <  1:2055819/93‑1 (MQ=255)
                       gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                                                  <  1:3254248/93‑1 (MQ=255)
                        tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                                                                 <  1:4613932/93‑1 (MQ=255)
                         cGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacg                                                                <  1:378303/93‑1 (MQ=255)
                          gATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                                                               <  2:2638556/93‑1 (MQ=255)
                          gATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                                                               <  2:1839035/93‑1 (MQ=255)
                          gATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                                                               <  1:2502888/93‑1 (MQ=255)
                           aTGGCGGGGAAAGGCTGCATTATGGGCAGTGGGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                                                              >  2:430081/1‑93 (MQ=255)
                           aTGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                                                              <  2:2255970/93‑1 (MQ=255)
                             ggcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                                                            <  2:251145/89‑1 (MQ=255)
                              gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgcg                                                           <  1:1307376/90‑1 (MQ=255)
                              gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgcg                                                           >  1:1452411/4‑93 (MQ=255)
                              gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgcg                                                           >  1:4103495/4‑93 (MQ=255)
                              gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgcg                                                           <  2:1336706/90‑1 (MQ=255)
                                ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCggg                                                         >  1:338655/2‑92 (MQ=255)
                                ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                                                         <  1:3202892/92‑1 (MQ=255)
                                ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                                                         <  2:3511676/92‑1 (MQ=255)
                                  ggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                                                       >  2:1678308/1‑93 (MQ=255)
                                    aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCg                                                     >  1:3156432/5‑93 (MQ=255)
                                     aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                                                    >  2:54098/4‑93 (MQ=255)
                                      aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                                                   >  1:3970555/3‑93 (MQ=255)
                                       ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                  <  1:2349192/92‑1 (MQ=255)
                                         cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa                                                >  2:1611728/1‑93 (MQ=255)
                                           gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCCTTATc                                              >  1:2716988/1‑93 (MQ=255)
                                           gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              <  2:4100270/93‑1 (MQ=255)
                                           gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              <  2:1120316/93‑1 (MQ=255)
                                           gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              <  1:1236797/93‑1 (MQ=255)
                                            cATTAGGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             <  2:3728277/93‑1 (MQ=255)
                                              ttATGGGCAGTGCGCACCCGCGTCTGAAAGACCTTCATCCCGAGCTGGGAGTGATTGGTACTAATGCCGACGACGCGGTGACGCATTCTCTGc                                           >  2:3422385/1‑93 (MQ=255)
                                              ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           >  1:3867303/1‑93 (MQ=255)
                                              ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           >  2:1487235/1‑93 (MQ=255)
                                              ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           <  1:2283480/93‑1 (MQ=255)
                                                aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt                                         >  2:84832/1‑93 (MQ=255)
                                                  gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       >  1:661077/1‑93 (MQ=255)
                                                  gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       <  2:124529/93‑1 (MQ=255)
                                                   ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      <  1:710534/93‑1 (MQ=255)
                                                   ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      >  2:1733177/1‑93 (MQ=255)
                                                   ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      <  1:3503039/93‑1 (MQ=255)
                                                    gCAGTGCGCACCAGCGGCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc                                     <  2:199647/93‑1 (MQ=255)
                                                        tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                 <  1:3090819/89‑1 (MQ=255)
                                                        tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                 >  1:1907258/5‑93 (MQ=255)
                                                         gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAt                                <  2:1423076/90‑1 (MQ=255)
                                                          cgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAtt                               <  1:3736977/91‑1 (MQ=255)
                                                              ccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc                           <  2:2112539/93‑1 (MQ=255)
                                                               caGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                          >  2:377566/1‑93 (MQ=255)
                                                                aGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                         <  2:3033473/93‑1 (MQ=255)
                                                                 gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTa                        >  1:3235978/1‑93 (MQ=255)
                                                                 gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGATTGGTACTAATGCCGCCGACGCGGTGCCGCATTATCTACGTAAACCCTATTTATCGTa                        >  2:2981560/1‑93 (MQ=255)
                                                                   gTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                      >  1:2629724/1‑93 (MQ=255)
                                                                   gTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                      <  1:88078/93‑1 (MQ=255)
                                                                    tCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAtt                     >  2:4628032/1‑92 (MQ=255)
                                                                    tCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                     >  1:2802913/1‑93 (MQ=255)
                                                                     cTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCg                    <  2:3956877/93‑1 (MQ=255)
                                                                      tGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   >  1:3019917/1‑93 (MQ=255)
                                                                      tGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   <  1:2426117/93‑1 (MQ=255)
                                                                        aaaGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                 >  2:3360654/1‑93 (MQ=255)
                                                                        aaaGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                 >  1:1255972/1‑93 (MQ=255)
                                                                         aaGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCt                >  2:1277304/1‑93 (MQ=255)
                                                                           gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              >  2:901611/1‑93 (MQ=255)
                                                                           gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              >  2:1316247/1‑93 (MQ=255)
                                                                            aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa             <  2:3675017/93‑1 (MQ=255)
                                                                            aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa             >  2:2437101/1‑93 (MQ=255)
                                                                            aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa             <  1:1336225/93‑1 (MQ=255)
                                                                             ccTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAt            >  2:4146424/1‑93 (MQ=255)
                                                                              cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           <  1:1671891/93‑1 (MQ=255)
                                                                              cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           >  2:2158964/1‑93 (MQ=255)
                                                                              cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           <  1:3181955/93‑1 (MQ=255)
                                                                              cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           <  1:153524/93‑1 (MQ=255)
                                                                               ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          >  1:1046354/1‑93 (MQ=255)
                                                                               ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          >  2:3903225/1‑93 (MQ=255)
                                                                               ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          >  1:122801/1‑93 (MQ=255)
                                                                                tCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg         >  1:3682176/1‑93 (MQ=255)
                                                                                tCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg         <  2:1563653/93‑1 (MQ=255)
                                                                                 cATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTgg        <  2:3629692/93‑1 (MQ=255)
                                                                                  aTCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       <  2:660327/93‑1 (MQ=255)
                                                                                  aTCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       <  1:4130012/93‑1 (MQ=255)
                                                                                  aTCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       >  2:2362172/1‑93 (MQ=255)
                                                                                   tCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTc      >  1:750654/1‑93 (MQ=255)
                                                                                   tCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTc      <  2:1015377/93‑1 (MQ=255)
                                                                                     ccGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAg    <  1:59102/93‑1 (MQ=255)
                                                                                      cGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGt   <  1:1278552/93‑1 (MQ=255)
                                                                                       gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt  <  1:4283695/93‑1 (MQ=255)
                                                                                       gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt  <  2:735095/93‑1 (MQ=255)
                                                                                            |                                                                                       
GGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008435‑4008611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC  >  NC_000913/4008435‑4008617
                                                                                              |                                                                                            
GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                             >  SRR4302535.1072564/1‑94 (MQ=60)
GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                             >  SRR4302535.4193544/1‑94 (MQ=60)
GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                             <  SRR4302535.1280275/94‑1 (MQ=60)
  GATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                                                           <  SRR4302535.3230716/94‑4 (MQ=60)
  GATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                                                           <  SRR4302535.1253032/94‑4 (MQ=60)
   ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                           <  SRR4302535.2667620/94‑1 (MQ=60)
   ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                           >  SRR4302535.3196930/1‑94 (MQ=60)
    TGAACGACGCGGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                                                          <  SRR4302535.3097308/94‑1 (MQ=60)
    TGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                                                          >  SRR4302535.985089/1‑94 (MQ=60)
    TGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                                                          <  SRR4302535.3081714/94‑1 (MQ=60)
      AACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                        <  SRR4302535.3246209/94‑1 (MQ=60)
       ACGACGCCGAGATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                       <  SRR4302535.454719/94‑1 (MQ=60)
       ACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                       <  SRR4302535.1126090/94‑1 (MQ=60)
         GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                     <  SRR4302535.4471707/94‑1 (MQ=60)
         GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                     >  SRR4302535.917468/1‑94 (MQ=60)
          ACGCCGAAATGCTCTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                    <  SRR4302535.2697397/94‑1 (MQ=60)
             CCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                 >  SRR4302535.4202139/1‑94 (MQ=60)
             CCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                 >  SRR4302535.1400195/1‑94 (MQ=60)
              CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                <  SRR4302535.2293395/94‑1 (MQ=60)
               GAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                                                               >  SRR4302535.1820182/1‑94 (MQ=60)
                AAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                              >  SRR4302535.3415029/1‑94 (MQ=60)
                AAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                              >  SRR4302535.2344464/1‑94 (MQ=60)
                 AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                             >  SRR4302535.2651406/1‑94 (MQ=60)
                 AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                             <  SRR4302535.3794835/94‑1 (MQ=60)
                 AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                             <  SRR4302535.897072/94‑1 (MQ=60)
                 AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                             <  SRR4302535.3058682/94‑1 (MQ=60)
                  ATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                            >  SRR4302535.2122399/1‑94 (MQ=60)
                   TGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                           <  SRR4302535.359317/94‑1 (MQ=60)
                   TGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                           >  SRR4302535.3046173/1‑94 (MQ=60)
                    GCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGGGCACCAGCGTCTGAAAGACCTTCATACCGAGCTGGAAGGGATTGGTACTTATGCC                                                                          >  SRR4302535.3150404/1‑94 (MQ=57)
                     CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                         <  SRR4302535.1474228/94‑1 (MQ=60)
                     CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                         >  SRR4302535.1702494/1‑94 (MQ=60)
                      TGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGTAGTGATTGGTACTAATGCCGA                                                                        >  SRR4302535.926616/1‑94 (MQ=60)
                       GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                       <  SRR4302535.2070629/94‑1 (MQ=60)
                       GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                       <  SRR4302535.3276858/94‑1 (MQ=60)
                        TCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                                                      <  SRR4302535.4645279/94‑1 (MQ=60)
                         CGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                                                     <  SRR4302535.380924/94‑1 (MQ=60)
                          GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                    <  SRR4302535.2520935/94‑1 (MQ=60)
                           ATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                   <  SRR4302535.685392/94‑1 (MQ=60)
                           ATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                   <  SRR4302535.3783146/94‑1 (MQ=60)
                           ATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                   >  SRR4302535.811241/1‑94 (MQ=60)
                             GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                 <  SRR4302535.3247612/94‑1 (MQ=60)
                             GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                 >  SRR4302535.1462804/1‑94 (MQ=60)
                             GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                 >  SRR4302535.4131587/1‑94 (MQ=60)
                               gcggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGG                                                                <  SRR4302535.1316714/84‑1 (MQ=60)
                                cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                               >  SRR4302535.3756306/10‑94 (MQ=60)
                                cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                               <  SRR4302535.1236952/85‑1 (MQ=60)
                                cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                               <  SRR4302535.801094/85‑1 (MQ=60)
                                cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGG                                                               >  SRR4302535.341000/10‑94 (MQ=60)
                                 ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                              <  SRR4302535.3604091/86‑1 (MQ=60)
                                 ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                              <  SRR4302535.4492088/86‑1 (MQ=60)
                                 ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                              <  SRR4302535.3225069/86‑1 (MQ=60)
                                 GG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                            >  SRR4302535.3849761/1‑94 (MQ=60)
                                  G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                                                           >  SRR4302535.3178349/1‑94 (MQ=60)
                                     AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                         >  SRR4302535.941217/1‑94 (MQ=60)
                                     AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                         >  SRR4302535.3997677/1‑94 (MQ=60)
                                       G‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                       <  SRR4302535.1830240/94‑1 (MQ=60)
                                       G‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                       <  SRR4302535.2366038/94‑1 (MQ=60)
                                         GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                      <  SRR4302535.2522363/94‑1 (MQ=60)
                                          CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                     >  SRR4302535.1065684/1‑94 (MQ=60)
                                          CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                     <  SRR4302535.3833812/94‑1 (MQ=60)
                                           TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCCTTATC                                                    >  SRR4302535.2736397/1‑94 (MQ=60)
                                           TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                    >  SRR4302535.4142286/1‑94 (MQ=60)
                                           TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                    >  SRR4302535.624483/1‑94 (MQ=60)
                                            GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                   <  SRR4302535.1245631/94‑1 (MQ=60)
                                            GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                   <  SRR4302535.745094/94‑1 (MQ=60)
                                            GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                   <  SRR4302535.4302279/94‑1 (MQ=60)
                                              ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                 >  SRR4302535.3893893/1‑94 (MQ=60)
                                               TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                <  SRR4302535.2217515/94‑1 (MQ=60)
                                               TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                <  SRR4302535.2502661/94‑1 (MQ=60)
                                               TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                <  SRR4302535.2299796/94‑1 (MQ=60)
                                                  TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                             >  SRR4302535.665689/1‑94 (MQ=60)
                                                  TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                             <  SRR4302535.3239698/94‑1 (MQ=60)
                                                  TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                             >  SRR4302535.2387404/1‑94 (MQ=60)
                                                   GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                            >  SRR4302535.3313448/1‑94 (MQ=60)
                                                    GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                           <  SRR4302535.715520/94‑1 (MQ=60)
                                                    GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                           <  SRR4302535.3527387/94‑1 (MQ=60)
                                                     GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT                                          <  SRR4302535.2423258/94‑1 (MQ=60)
                                                     GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT                                          <  SRR4302535.4400185/94‑1 (MQ=60)
                                                       AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                        >  SRR4302535.1720175/1‑94 (MQ=60)
                                                       AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                        <  SRR4302535.4028998/94‑1 (MQ=60)
                                                        gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                       <  SRR4302535.785888/89‑1 (MQ=60)
                                                        gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                       >  SRR4302535.1920983/6‑94 (MQ=60)
                                                         tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                      >  SRR4302535.2720625/5‑94 (MQ=60)
                                                         tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                      <  SRR4302535.3112344/90‑1 (MQ=60)
                                                          gcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                     <  SRR4302535.51374/91‑1 (MQ=60)
                                                           cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                    >  SRR4302535.4002185/3‑94 (MQ=60)
                                                           cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                    <  SRR4302535.1673798/92‑1 (MQ=60)
                                                           cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                    <  SRR4302535.3762841/92‑1 (MQ=60)
                                                             CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                  <  SRR4302535.3976830/94‑1 (MQ=60)
                                                              ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                 <  SRR4302535.315977/94‑1 (MQ=60)
                                                               CCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCG                                <  SRR4302535.1345148/94‑1 (MQ=60)
                                                                CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATAGT                               >  SRR4302535.1219744/1‑94 (MQ=60)
                                                                 AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                              >  SRR4302535.4587448/1‑94 (MQ=60)
                                                                 AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                              >  SRR4302535.1688575/1‑94 (MQ=60)
                                                                 AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                              >  SRR4302535.708517/1‑94 (MQ=60)
                                                                 AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                              >  SRR4302535.3258440/1‑94 (MQ=60)
                                                                   CGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAT                            >  SRR4302535.2648566/1‑94 (MQ=60)
                                                                    GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                           <  SRR4302535.88697/94‑1 (MQ=60)
                                                                    GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                           >  SRR4302535.2822847/1‑94 (MQ=60)
                                                                     TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTTCTGATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                          <  SRR4302535.4715028/94‑1 (MQ=60)
                                                                      CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                         <  SRR4302535.151678/94‑1 (MQ=60)
                                                                      CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                         >  SRR4302535.3041067/1‑94 (MQ=60)
                                                                       TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                        <  SRR4302535.2443596/94‑1 (MQ=60)
                                                                       TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                        <  SRR4302535.2782283/94‑1 (MQ=60)
                                                                        GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                       >  SRR4302535.1264954/1‑94 (MQ=60)
                                                                          AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                     <  SRR4302535.1324598/94‑1 (MQ=60)
                                                                           AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                    >  SRR4302535.3274871/1‑94 (MQ=60)
                                                                            GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                   <  SRR4302535.1303135/94‑1 (MQ=60)
                                                                             ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                  <  SRR4302535.1345757/94‑1 (MQ=60)
                                                                             ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                  <  SRR4302535.2052043/94‑1 (MQ=60)
                                                                               CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                <  SRR4302535.3203946/94‑1 (MQ=60)
                                                                               CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                >  SRR4302535.1053771/1‑94 (MQ=60)
                                                                               CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                >  SRR4302535.4411531/1‑94 (MQ=60)
                                                                               CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                >  SRR4302535.123665/1‑94 (MQ=60)
                                                                               CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                <  SRR4302535.154602/94‑1 (MQ=60)
                                                                               CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                <  SRR4302535.1683992/94‑1 (MQ=60)
                                                                                TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG               <  SRR4302535.3271792/94‑1 (MQ=60)
                                                                                TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG               >  SRR4302535.3707658/1‑94 (MQ=60)
                                                                                TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG               <  SRR4302535.3022040/94‑1 (MQ=60)
                                                                                 TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG              >  SRR4302535.4121201/1‑94 (MQ=60)
                                                                                  CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAACCTCTATTTATCGTAATCGTTCTTTATTTGGT             <  SRR4302535.1160140/94‑1 (MQ=60)
                                                                                  CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT             <  SRR4302535.124560/94‑1 (MQ=60)
                                                                                   ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC            >  SRR4302535.755945/1‑94 (MQ=60)
                                                                                   ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC            <  SRR4302535.4158272/94‑1 (MQ=60)
                                                                                     CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG          >  SRR4302535.1075468/1‑94 (MQ=60)
                                                                                     CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG          <  SRR4302535.4175833/94‑1 (MQ=60)
                                                                                      CCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGT         <  SRR4302535.59536/94‑1 (MQ=60)
                                                                                       CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT        <  SRR4302535.3212828/94‑1 (MQ=60)
                                                                                       CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT        <  SRR4302535.1287682/94‑1 (MQ=60)
                                                                                        GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG       <  SRR4302535.2602608/94‑1 (MQ=60)
                                                                                        GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG       <  SRR4302535.4313001/94‑1 (MQ=60)
                                                                                         AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT      <  SRR4302535.2228099/94‑1 (MQ=60)
                                                                                         AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT      >  SRR4302535.516387/1‑94 (MQ=60)
                                                                                         AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT      >  SRR4302535.4641278/1‑94 (MQ=60)
                                                                                         AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT      <  SRR4302535.2870392/94‑1 (MQ=60)
                                                                                          GCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTC     >  SRR4302535.4597247/1‑94 (MQ=60)
                                                                                          GCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTC     >  SRR4302535.1318778/1‑94 (MQ=60)
                                                                                           ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA    >  SRR4302535.3695799/4‑94 (MQ=60)
                                                                                           ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA    >  SRR4302535.2881633/4‑94 (MQ=60)
                                                                                            tgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA   <  SRR4302535.1057296/92‑1 (MQ=60)
                                                                                            G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC  >  SRR4302535.2860584/1‑94 (MQ=60)
                                                                                              |                                                                                            
GGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC  >  NC_000913/4008435‑4008617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑