Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,525 +G intergenic (+92/‑141) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,5241.G100.0% 1118.8 / NA 347intergenic (+91/‑142)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (161/186);  total (161/186)

Only 100 of 380 total aligned reads displayed.

CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGAAA‑GCTGCATTATGGGCAGTG‑GCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008434‑4008611
                                                                                               |                                                                                       
cGGGATGAACGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                                                          >  2:4069166/1‑93 (MQ=255)
cGGGATGAACGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                                                          <  1:7232807/93‑1 (MQ=255)
 gggATGAACGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                                                        >  1:5876092/1‑93 (MQ=255)
  ggATGAACGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                       >  1:6182088/1‑92 (MQ=255)
   gATGAACGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                                                      >  1:1429259/1‑91 (MQ=255)
   gATGAACGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                                                      >  2:3424089/1‑91 (MQ=255)
    aTGAACGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                     >  2:6032102/1‑90 (MQ=255)
     tGAACGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                                                    <  2:6595280/93‑5 (MQ=255)
       aaCGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                                                   >  1:7658368/1‑93 (MQ=255)
       aaCGACGCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                                                   >  2:4503784/1‑93 (MQ=255)
        acgacgCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                                                  <  2:7380003/93‑1 (MQ=255)
        acgacgCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                                                  <  2:6265685/93‑1 (MQ=255)
         cgacgCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                                                                 >  1:1715506/1‑93 (MQ=255)
         cgacgCCGAAATGCTGTCGATGGC‑GTGGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                                                                 <  1:4664415/93‑1 (MQ=255)
          gacgCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                                                                >  1:6261266/1‑93 (MQ=255)
            cgCCGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                                                              <  2:2382457/93‑1 (MQ=255)
              ccGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                                                            <  1:5430293/93‑1 (MQ=255)
               cGAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                                                           <  2:522793/93‑1 (MQ=255)
                gAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                                                          >  1:6405223/1‑93 (MQ=255)
                gAAATGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                                                          >  1:4371889/1‑93 (MQ=255)
                 aaaTGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                                                         >  1:7863633/1‑93 (MQ=255)
                  aaTGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                                                        <  2:3206015/93‑1 (MQ=255)
                   aTGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                                                       <  2:4371888/93‑1 (MQ=255)
                   aTGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                                                       >  2:3903730/1‑93 (MQ=255)
                   aTGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTAAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                                                       <  2:2092686/93‑1 (MQ=255)
                    tGCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                                                      <  2:7880347/93‑1 (MQ=255)
                     gCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                                                     >  1:5144967/1‑93 (MQ=255)
                     gCTGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                                                     >  1:2103308/1‑93 (MQ=255)
                       tGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                                                   >  1:5691030/1‑93 (MQ=255)
                       tGTCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                                                   <  2:1402237/93‑1 (MQ=255)
                         tCGATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                                                                 >  2:4268867/1‑93 (MQ=255)
                           gATGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                                                               <  1:534086/93‑1 (MQ=255)
                            aTGGCGG‑GGAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                                                              <  1:679844/93‑1 (MQ=255)
                              ggcgg‑ggAAAGGCTGCATTATGGGCAGTGCGCCA‑CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                                                            <  1:6118673/89‑1 (MQ=255)
                              ggcgg‑ggAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                                                            >  2:6342295/5‑93 (MQ=255)
                              ggcgg‑ggAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                                                            <  2:1249801/89‑1 (MQ=255)
                                cgg‑ggAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                                                          <  2:4833486/91‑1 (MQ=255)
                                cgg‑ggAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                                                          <  1:1247214/91‑1 (MQ=255)
                                  g‑ggAAAGGCTGGATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                                                        <  2:5411092/93‑1 (MQ=255)
                                    ggAAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                                                       <  2:6505524/93‑1 (MQ=255)
                                       aaggCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                                                    >  1:7754870/4‑93 (MQ=255)
                                         ggCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                  <  1:877714/92‑1 (MQ=255)
                                          gCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt                                                 >  1:4838368/1‑93 (MQ=255)
                                           cTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa                                                <  2:5713371/93‑1 (MQ=255)
                                            tGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               <  2:403576/93‑1 (MQ=255)
                                            tGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               >  1:7562367/1‑93 (MQ=255)
                                            tGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               >  1:2628251/1‑93 (MQ=255)
                                             gCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              >  1:6575702/1‑93 (MQ=255)
                                             gCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              >  2:2846964/1‑93 (MQ=255)
                                              cATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             >  2:1583881/1‑93 (MQ=255)
                                              cATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             <  1:2476241/93‑1 (MQ=255)
                                              cATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             <  2:4542945/93‑1 (MQ=255)
                                                ttATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           <  2:7237980/93‑1 (MQ=255)
                                                ttATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           >  1:1084314/1‑93 (MQ=255)
                                                ttATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           <  1:338772/93‑1 (MQ=255)
                                                ttATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           >  1:4289329/1‑93 (MQ=255)
                                                ttATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCAGACGACGCGGTGCCGCATTATCTGc                                           <  1:6936037/93‑1 (MQ=255)
                                                 tATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg                                          >  1:7320193/1‑93 (MQ=255)
                                                   tGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                        >  2:4751093/1‑93 (MQ=255)
                                                   tGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                        >  1:2168253/1‑93 (MQ=255)
                                                   tGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                        >  2:5047312/1‑93 (MQ=255)
                                                   tGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                        >  2:1748615/1‑93 (MQ=255)
                                                    gggCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       <  2:2203663/93‑1 (MQ=255)
                                                    gggCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       >  1:5860415/1‑93 (MQ=255)
                                                    gggCAGTA‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       <  2:7449042/93‑1 (MQ=255)
                                                     ggCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      <  1:2831314/93‑1 (MQ=255)
                                                      gCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc                                     >  1:4074173/1‑93 (MQ=255)
                                                       cAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct                                    <  1:5224807/93‑1 (MQ=255)
                                                           tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                 >  2:1022872/5‑93 (MQ=255)
                                                           tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                 <  1:3958639/89‑1 (MQ=255)
                                                              gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAttt                              >  2:3011864/2‑93 (MQ=255)
                                                              gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAttt                              <  2:6187625/92‑1 (MQ=255)
                                                              gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAttt                              <  2:575428/92‑1 (MQ=255)
                                                               caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             <  1:5517056/93‑1 (MQ=255)
                                                               caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             <  2:1900714/93‑1 (MQ=255)
                                                               caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             <  1:326276/93‑1 (MQ=255)
                                                                cccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                            <  2:3477739/92‑1 (MQ=255)
                                                                accaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                            >  2:6682862/1‑93 (MQ=255)
                                                                accaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                            >  1:3028198/1‑93 (MQ=255)
                                                                  caGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                          <  1:1538945/93‑1 (MQ=255)
                                                                   aGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                         >  2:7603628/1‑93 (MQ=255)
                                                                      gTCTGAAAGACTTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGGCGCATTATCTGCGTAAACTCTATTTATCGTAAt                      <  2:6404788/93‑1 (MQ=255)
                                                                      gTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                      <  1:3179688/93‑1 (MQ=255)
                                                                       gcTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                     <  1:6065592/92‑1 (MQ=255)
                                                                        cTGAAATACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCg                    <  1:1975079/93‑1 (MQ=255)
                                                                         tGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   >  1:3482997/1‑93 (MQ=255)
                                                                         tGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   >  1:4479584/1‑93 (MQ=255)
                                                                         tGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   >  2:4177519/1‑93 (MQ=255)
                                                                             aGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt               <  2:3746606/93‑1 (MQ=255)
                                                                               aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa             <  1:3600034/93‑1 (MQ=255)
                                                                               aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa             >  1:4789935/1‑93 (MQ=255)
                                                                                 cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           <  2:825140/93‑1 (MQ=255)
                                                                                 cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           >  1:1019087/1‑93 (MQ=255)
                                                                                 cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           <  2:6793203/93‑1 (MQ=255)
                                                                                 cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           >  1:2321087/1‑93 (MQ=255)
                                                                                   tCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg         <  1:7102675/93‑1 (MQ=255)
                                                                                     aTCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       >  2:6934701/1‑93 (MQ=255)
                                                                                     aTCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       >  2:2149993/1‑93 (MQ=255)
                                                                                       cccGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCa     <  1:6469903/93‑1 (MQ=255)
                                                                                        ccGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAg    >  2:1667431/1‑93 (MQ=255)
                                                                                               |                                                                                       
CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGAAA‑GCTGCATTATGGGCAGTG‑GCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008434‑4008611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 221 total aligned reads displayed.
CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTG‑GCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618
                                                                                                |                                                                                             
CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               >  SRR4302543.7298166/1‑94 (MQ=60)
CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               <  SRR4302543.7302850/94‑1 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCTAGCT‑GG                                                                                              >  SRR4302543.4845302/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302543.5095229/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302543.6240515/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302543.7606789/1‑94 (MQ=60)
  GGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                                                             >  SRR4302543.5919794/1‑94 (MQ=60)
  GGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                                                             >  SRR4302543.624410/1‑94 (MQ=60)
  GGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                                                             >  SRR4302543.1442188/1‑94 (MQ=60)
  GGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                                                             >  SRR4302543.6546049/1‑94 (MQ=60)
   GATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                                                            <  SRR4302543.7732543/94‑4 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            >  SRR4302543.4824059/1‑94 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            >  SRR4302543.7075981/1‑94 (MQ=60)
      GAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                                                          <  SRR4302543.2909976/94‑1 (MQ=60)
      GAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                                                          >  SRR4302543.7730964/1‑94 (MQ=60)
      GAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                                                          >  SRR4302543.10788/1‑94 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         <  SRR4302543.559375/94‑1 (MQ=60)
         CGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       <  SRR4302543.2370731/94‑1 (MQ=60)
         CGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       >  SRR4302543.2649934/1‑94 (MQ=60)
         CGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       >  SRR4302543.6320317/1‑94 (MQ=60)
          GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      <  SRR4302543.2326281/94‑1 (MQ=60)
          GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      <  SRR4302543.5203767/94‑1 (MQ=60)
          GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      <  SRR4302543.7988034/94‑1 (MQ=60)
          GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      >  SRR4302543.541049/1‑94 (MQ=60)
           ACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                     <  SRR4302543.5302161/94‑1 (MQ=60)
            CGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                                                    >  SRR4302543.5745832/1‑94 (MQ=60)
              CCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                  <  SRR4302543.536243/94‑1 (MQ=60)
              CCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                  <  SRR4302543.6204208/94‑1 (MQ=60)
              CCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                  <  SRR4302543.5479630/94‑1 (MQ=60)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302543.4411483/1‑94 (MQ=60)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302543.6465545/1‑94 (MQ=60)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302543.3122017/1‑94 (MQ=60)
                GAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                                                                >  SRR4302543.7937687/1‑94 (MQ=60)
                GAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                                                                <  SRR4302543.1272658/94‑1 (MQ=60)
                 AAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                               >  SRR4302543.5777097/1‑94 (MQ=60)
                  AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              <  SRR4302543.152812/94‑1 (MQ=60)
                  AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              <  SRR4302543.1146385/94‑1 (MQ=60)
                  AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              <  SRR4302543.7101858/94‑1 (MQ=60)
                   ATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             <  SRR4302543.5773731/94‑1 (MQ=60)
                    TGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                            >  SRR4302543.713379/1‑94 (MQ=60)
                    TGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                            >  SRR4302543.5191478/1‑94 (MQ=60)
                    TGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                            >  SRR4302543.2122130/1‑94 (MQ=60)
                    TGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                            <  SRR4302543.1322505/94‑1 (MQ=60)
                     GCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           >  SRR4302543.3157507/1‑94 (MQ=60)
                      GTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCTCCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302543.2471202/94‑1 (MQ=55)
                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAGGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302543.6891097/1‑94 (MQ=60)
                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302543.4489452/94‑1 (MQ=60)
                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302543.5742968/1‑94 (MQ=60)
                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302543.7638120/1‑94 (MQ=60)
                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302543.2098495/94‑1 (MQ=60)
                       TGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                                                         >  SRR4302543.397923/1‑94 (MQ=60)
                        GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                        >  SRR4302543.6728615/1‑94 (MQ=60)
                        GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                        <  SRR4302543.7672238/94‑1 (MQ=60)
                        GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                        >  SRR4302543.7560842/1‑94 (MQ=60)
                        GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                        <  SRR4302543.7683712/94‑1 (MQ=60)
                          CGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                                                      >  SRR4302543.912620/1‑94 (MQ=60)
                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302543.3149384/94‑1 (MQ=60)
                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302543.2131549/94‑1 (MQ=60)
                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302543.3525775/94‑1 (MQ=60)
                            ATGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                    >  SRR4302543.3976406/1‑94 (MQ=60)
                             TGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   <  SRR4302543.5734891/94‑1 (MQ=60)
                             TGGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   <  SRR4302543.1278147/94‑1 (MQ=60)
                              GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCCA‑CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                  <  SRR4302543.6176628/94‑1 (MQ=60)
                              GGCGGGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                  <  SRR4302543.7385665/94‑1 (MQ=60)
                                 cggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                <  SRR4302543.1258409/85‑1 (MQ=60)
                                 cggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                <  SRR4302543.2133712/85‑1 (MQ=60)
                                  gggtaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               <  SRR4302543.7244095/86‑1 (MQ=60)
                                  ggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               <  SRR4302543.6779977/86‑1 (MQ=60)
                                  GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                             <  SRR4302543.1872261/94‑1 (MQ=60)
                                  GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                             >  SRR4302543.2307215/1‑94 (MQ=60)
                                  GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTGGTACTAATGCCGACGACGCGGTGCC                                                             >  SRR4302543.4127525/1‑94 (MQ=60)
                                       AG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         <  SRR4302543.7898816/94‑1 (MQ=60)
                                       AG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         >  SRR4302543.6903147/1‑94 (MQ=60)
                                        G‑GCTGCATTATGGGCAGTG‑CGCACCCGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGACGACGACGCGGTGCCGCATT                                                        >  SRR4302543.6969584/1‑94 (MQ=60)
                                        G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                        <  SRR4302543.885511/94‑1 (MQ=60)
                                        G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                        >  SRR4302543.4882494/1‑94 (MQ=60)
                                        G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                        >  SRR4302543.3281012/1‑94 (MQ=60)
                                        G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGGACTAATGCCGACGACGCGGTGCCGCATT                                                        >  SRR4302543.3281011/1‑94 (MQ=60)
                                          GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                       <  SRR4302543.2409399/94‑1 (MQ=60)
                                          GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                       <  SRR4302543.6517378/94‑1 (MQ=60)
                                           CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      >  SRR4302543.2652034/1‑94 (MQ=60)
                                           CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      >  SRR4302543.3510758/1‑94 (MQ=60)
                                           CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      >  SRR4302543.7815043/1‑94 (MQ=60)
                                           CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      >  SRR4302543.7634347/1‑94 (MQ=60)
                                           CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      >  SRR4302543.201476/1‑94 (MQ=60)
                                           CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      <  SRR4302543.2806068/94‑1 (MQ=60)
                                            TGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                     >  SRR4302543.5424882/1‑94 (MQ=60)
                                            TGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                     >  SRR4302543.6637623/1‑94 (MQ=60)
                                             GCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    >  SRR4302543.4432785/1‑94 (MQ=60)
                                             GCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    <  SRR4302543.4844220/94‑1 (MQ=60)
                                             GCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    >  SRR4302543.7738255/1‑94 (MQ=60)
                                              CATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302543.2498631/94‑1 (MQ=60)
                                               ATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  >  SRR4302543.4328323/1‑94 (MQ=60)
                                               ATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302543.1227199/94‑1 (MQ=60)
                                               ATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  >  SRR4302543.1094057/1‑94 (MQ=60)
                                               ATTATGGGCAGTG‑CGCACAAGAGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  >  SRR4302543.3242304/1‑94 (MQ=60)
                                                TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 >  SRR4302543.5547157/1‑94 (MQ=60)
                                                TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302543.341883/94‑1 (MQ=60)
                                                TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302543.5527107/94‑1 (MQ=60)
                                                TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302543.7922343/94‑1 (MQ=60)
                                                TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 >  SRR4302543.7390700/1‑94 (MQ=60)
                                                TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302543.6570235/94‑1 (MQ=60)
                                                TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCAGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302543.7001276/94‑1 (MQ=60)
                                                 TATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT                                                >  SRR4302543.4131686/1‑94 (MQ=60)
                                                 TATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT                                                <  SRR4302543.3654358/94‑1 (MQ=60)
                                                 TATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATAGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT                                                >  SRR4302543.4131685/1‑94 (MQ=60)
                                                  ATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                               >  SRR4302543.5917811/1‑94 (MQ=60)
                                                  ATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                               >  SRR4302543.2187682/1‑94 (MQ=60)
                                                   TGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302543.5913754/1‑94 (MQ=60)
                                                   TGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302543.5913752/1‑94 (MQ=60)
                                                   TGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302543.5191479/1‑94 (MQ=60)
                                                    GGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             <  SRR4302543.152261/94‑1 (MQ=60)
                                                     GGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            >  SRR4302543.4110678/1‑94 (MQ=60)
                                                     GGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            <  SRR4302543.2857216/94‑1 (MQ=60)
                                                      GCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT                                           <  SRR4302543.3317571/94‑1 (MQ=60)
                                                       CAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC                                          <  SRR4302543.5271848/94‑1 (MQ=60)
                                                       CAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC                                          >  SRR4302543.491323/1‑94 (MQ=60)
                                                        AGTG‑CGCACCAGCGTCTGAAATACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGACGACTACGCGGTGCCGCATTATCTGCGTAAACTCT                                         >  SRR4302543.3847547/1‑94 (MQ=60)
                                                        AGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                         >  SRR4302543.4642525/1‑94 (MQ=60)
                                                          gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302543.4873651/89‑1 (MQ=60)
                                                           tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                       <  SRR4302543.5257159/90‑1 (MQ=60)
                                                           tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                       <  SRR4302543.3993685/90‑1 (MQ=60)
                                                            gcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                      <  SRR4302543.621221/91‑1 (MQ=60)
                                                             cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     >  SRR4302543.6485519/3‑94 (MQ=60)
                                                             cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     <  SRR4302543.637384/92‑1 (MQ=60)
                                                             cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     <  SRR4302543.4590945/92‑1 (MQ=60)
                                                              GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                    >  SRR4302543.4024406/1‑94 (MQ=60)
                                                              GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATATA                                    >  SRR4302543.4087699/1‑94 (MQ=60)
                                                               CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302543.5567161/94‑1 (MQ=60)
                                                               CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302543.6278661/94‑1 (MQ=60)
                                                               CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   >  SRR4302543.3055554/1‑94 (MQ=60)
                                                               CACCAGCGTCTGAAAGACCTTCAGCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302543.2324533/94‑1 (MQ=60)
                                                                ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  >  SRR4302543.6278107/1‑94 (MQ=60)
                                                                ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  >  SRR4302543.812539/1‑94 (MQ=60)
                                                                  CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                <  SRR4302543.719402/94‑1 (MQ=60)
                                                                  CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                <  SRR4302543.1552728/94‑1 (MQ=60)
                                                                   AGCTTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                               <  SRR4302543.5612045/94‑1 (MQ=60)
                                                                    GCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAA                              <  SRR4302543.294222/94‑1 (MQ=60)
                                                                     CGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAT                             <  SRR4302543.5405338/94‑1 (MQ=60)
                                                                      GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            >  SRR4302543.5066971/1‑94 (MQ=60)
                                                                      GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            <  SRR4302543.3208353/94‑1 (MQ=60)
                                                                      GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGCGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            >  SRR4302543.1673934/1‑94 (MQ=60)
                                                                      GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGAGTCGCATAATCTGCGAAAACTCTATTTTTCGTGATC                            >  SRR4302543.4787181/1‑94 (MQ=60)
                                                                       TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           <  SRR4302543.138326/94‑1 (MQ=60)
                                                                       GCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           <  SRR4302543.6123070/94‑1 (MQ=60)
                                                                        CTGAAATACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          <  SRR4302543.1992789/94‑1 (MQ=60)
                                                                        CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          >  SRR4302543.4520260/1‑94 (MQ=60)
                                                                        CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          >  SRR4302543.3514192/1‑94 (MQ=60)
                                                                        CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          >  SRR4302543.3351908/1‑94 (MQ=60)
                                                                         TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         <  SRR4302543.3476969/94‑1 (MQ=60)
                                                                         TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         >  SRR4302543.2121238/1‑94 (MQ=60)
                                                                         TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         >  SRR4302543.6168223/1‑94 (MQ=60)
                                                                          GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                        >  SRR4302543.7837196/1‑94 (MQ=60)
                                                                          GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                        >  SRR4302543.6663289/1‑94 (MQ=60)
                                                                           AAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                       >  SRR4302543.4414260/1‑94 (MQ=60)
                                                                           AAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                       <  SRR4302543.5996551/94‑1 (MQ=60)
                                                                            AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302543.6393195/1‑94 (MQ=60)
                                                                            AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302543.6393194/1‑94 (MQ=60)
                                                                            AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      <  SRR4302543.4815509/94‑1 (MQ=60)
                                                                            AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302543.3931921/1‑94 (MQ=60)
                                                                            AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      <  SRR4302543.4408643/94‑1 (MQ=60)
                                                                              GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302543.3631497/94‑1 (MQ=60)
                                                                              GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    >  SRR4302543.4833713/1‑94 (MQ=60)
                                                                              GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302543.4665479/94‑1 (MQ=60)
                                                                               ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   >  SRR4302543.2859786/1‑94 (MQ=60)
                                                                               ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302543.3631805/94‑1 (MQ=60)
                                                                               ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302543.1626169/94‑1 (MQ=60)
                                                                               ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302543.5336975/94‑1 (MQ=60)
                                                                               ACCTGCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302543.1528656/94‑1 (MQ=60)
                                                                                CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  <  SRR4302543.955692/94‑1 (MQ=60)
                                                                                CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  >  SRR4302543.1028101/1‑94 (MQ=60)
                                                                                CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  >  SRR4302543.4542141/1‑94 (MQ=60)
                                                                                CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  >  SRR4302543.2342184/1‑94 (MQ=60)
                                                                                 CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302543.6174368/1‑94 (MQ=60)
                                                                                 CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302543.6112163/1‑94 (MQ=60)
                                                                                 CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302543.6098418/1‑94 (MQ=60)
                                                                                  TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                >  SRR4302543.2810228/1‑94 (MQ=60)
                                                                                  TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                <  SRR4302543.7341874/94‑1 (MQ=60)
                                                                                   TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG               <  SRR4302543.1113758/94‑1 (MQ=60)
                                                                                   TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG               <  SRR4302543.7171663/94‑1 (MQ=60)
                                                                                     ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC             >  SRR4302543.2684559/1‑94 (MQ=60)
                                                                                     ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC             >  SRR4302543.1892640/1‑94 (MQ=60)
                                                                                     ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC             >  SRR4302543.4341339/1‑94 (MQ=60)
                                                                                       CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           >  SRR4302543.1033424/1‑94 (MQ=60)
                                                                                       CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302543.3430077/94‑1 (MQ=60)
                                                                                       CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302543.2215106/94‑1 (MQ=60)
                                                                                       CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302543.6530855/94‑1 (MQ=60)
                                                                                          GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTAGTCAGTTG        <  SRR4302543.1634482/94‑1 (MQ=60)
                                                                                           AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       >  SRR4302543.3848386/1‑94 (MQ=60)
                                                                                           AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       <  SRR4302543.3157788/94‑1 (MQ=60)
                                                                                           AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       <  SRR4302543.7763109/94‑1 (MQ=60)
                                                                                           AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       <  SRR4302543.4262483/94‑1 (MQ=60)
                                                                                             ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     <  SRR4302543.3175398/91‑1 (MQ=60)
                                                                                             ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     >  SRR4302543.5821727/4‑94 (MQ=60)
                                                                                             ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     >  SRR4302543.6589605/4‑94 (MQ=60)
                                                                                             ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     <  SRR4302543.7018574/91‑1 (MQ=60)
                                                                                              tgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA    >  SRR4302543.3404307/3‑94 (MQ=60)
                                                                                              G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   <  SRR4302543.3845167/94‑1 (MQ=60)
                                                                                              G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   >  SRR4302543.7011833/1‑94 (MQ=60)
                                                                                                GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  SRR4302543.7558268/1‑94 (MQ=60)
                                                                                                |                                                                                             
CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTG‑GCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑