Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,013,772	+C	intergenic (‑53/‑246)	ysgA ← / → udp	hypothetical protein/uridine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,013,770	1	.	C	99.0%	635.0 / ‑1.3	201	intergenic (‑51/‑248)	ysgA/udp	hypothetical protein/uridine phosphorylase
Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (106/93); total (106/95)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 215 total aligned reads displayed.

GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTG  >  NC_000913/4013679‑4013857

gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑a                                                                                           <  1:4184691/93‑1 (MQ=255)

gcgTCGTCAGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑a                                                                                           <  2:2817111/93‑1 (MQ=255)

   tcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑ccc                                                                                       >  2:885723/1‑92 (MQ=255)

   tcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑ccc                                                                                       >  2:2690458/1‑92 (MQ=255)

     gtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑ccctt                                                                                     <  2:3140719/93‑4 (MQ=255)

      tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑ccctta                                                                                    <  2:23363/93‑5 (MQ=255)

        cGGGGTGTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGGCATGGTATTCTCCGT‑ACCC‑Ttata                                                                                  <  2:3809031/93‑1 (MQ=255)

        cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑Ttata                                                                                  >  2:3280926/1‑93 (MQ=255)

         ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATaa                                                                                 >  1:2226153/1‑93 (MQ=255)

         ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATaa                                                                                 <  1:3390095/93‑1 (MQ=255)

          gggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATaaa                                                                                <  1:745459/93‑1 (MQ=255)

          gggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATaaa                                                                                <  2:3506006/93‑1 (MQ=255)

           ggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATaaaa                                                                               >  1:331940/1‑93 (MQ=255)

            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATaaaaa                                                                              <  1:1416973/93‑1 (MQ=255)

            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATaaaaa                                                                              >  2:1210606/1‑93 (MQ=255)

             tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAAt                                                                             <  1:3509696/93‑1 (MQ=255)

             tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAAt                                                                             >  2:384987/1‑93 (MQ=255)

               tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGt                                                                           >  1:3742911/1‑93 (MQ=255)

               tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGt                                                                           <  1:4507078/93‑1 (MQ=255)

                gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGtt                                                                          <  1:2755833/93‑1 (MQ=255)

                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTg                                                                         >  1:2063897/1‑93 (MQ=255)

                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTg                                                                         <  2:338315/93‑1 (MQ=255)

                  aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTgc                                                                        <  2:620666/93‑1 (MQ=255)

                    gATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTgcgc                                                                      <  1:3809267/93‑1 (MQ=255)

                    gATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTgcgc                                                                      <  2:1519857/93‑1 (MQ=255)

                      tGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCaa                                                                    >  2:4512041/1‑93 (MQ=255)

                        gTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATg                                                                  >  1:1731977/1‑93 (MQ=255)

                          cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTGC‑CC‑TTATAAAAATGTTGCGCAATGtt                                                                <  2:3668164/93‑1 (MQ=255)

                           gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTa                                                               >  1:2992661/1‑93 (MQ=255)

                           gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTa                                                               <  1:1247831/93‑1 (MQ=255)

                              gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACt                                                            <  1:125319/93‑1 (MQ=255)

                               cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACta                                                           <  1:595155/93‑1 (MQ=255)

                                gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACtat                                                          <  2:4120111/93‑1 (MQ=255)

                                gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACtat                                                          <  1:3960258/93‑1 (MQ=255)

                                 agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACtata                                                         >  2:2329726/1‑93 (MQ=255)

                                 agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACtata                                                         >  1:4653232/1‑93 (MQ=255)

                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTc                                                      >  2:1131203/5‑93 (MQ=255)

                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTc                                                      >  1:725109/5‑93 (MQ=255)

                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTc                                                      <  1:2126811/89‑1 (MQ=255)

                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTc                                                      <  1:1661696/89‑1 (MQ=255)

                                     gcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCa                                                     >  1:670928/4‑93 (MQ=255)

                                       gaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGc                                                   >  2:666947/2‑93 (MQ=255)

                                       gaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGc                                                   >  1:4459356/2‑93 (MQ=255)

                                        aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCa                                                  >  2:2874134/1‑93 (MQ=255)

                                          gcagcaGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATg                                                >  1:3899191/1‑93 (MQ=255)

                                           cagcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGc                                               <  2:1294022/93‑1 (MQ=255)

                                            agcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGca                                              >  1:3681330/1‑93 (MQ=255)

                                            agcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGca                                              >  1:4295013/1‑93 (MQ=255)

                                             gcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaa                                             <  2:4646269/93‑1 (MQ=255)

                                             gcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaa                                             <  2:1684476/93‑1 (MQ=255)

                                              cagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATTcaac                                            <  1:1824439/93‑1 (MQ=255)

                                                ggTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaacaa                                          >  1:4364288/1‑93 (MQ=255)

                                                 gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACaaa                                         >  2:59851/1‑93 (MQ=255)

                                                 gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACaaa                                         <  1:4068547/93‑1 (MQ=255)

                                                 gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTTATAAAAACGTTGCGCAATGTTAACTATAGTCAGCATGCAACaaa                                         <  1:187011/93‑1 (MQ=255)

                                                  tGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAt                                        <  1:4078354/93‑1 (MQ=255)

                                                  tGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAt                                        <  1:2607014/93‑1 (MQ=255)

                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATc                                       <  1:2857916/93‑1 (MQ=255)

                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATc                                       <  1:1421582/93‑1 (MQ=255)

                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTAAACTATAGTCAGCATGCAACAAATc                                       <  2:3736226/93‑1 (MQ=255)

                                                    cAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATca                                      <  2:2556353/93‑1 (MQ=255)

                                                     aaaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcac                                     >  2:3433888/1‑93 (MQ=255)

                                                       aTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAt                                   <  1:2330419/93‑1 (MQ=255)

                                                       aTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAt                                   >  2:1445771/1‑93 (MQ=255)

                                                       aTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAt                                   <  2:3909523/93‑1 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  >  1:1689997/1‑93 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  <  2:2742471/93‑1 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  <  1:3567792/93‑1 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  <  2:1910348/93‑1 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAtt                                  >  1:1029126/1‑93 (MQ=255)

                                                          cAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGc                                >  2:1754569/1‑93 (MQ=255)

                                                          cAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGc                                >  1:844755/1‑93 (MQ=255)

                                                           aGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGcc                               <  2:4205668/93‑1 (MQ=255)

                                                            gATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCt                              >  2:1854048/1‑93 (MQ=255)

                                                            gATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCt                              >  2:3430542/1‑93 (MQ=255)

                                                            gATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCt                              <  1:2571888/93‑1 (MQ=255)

                                                                gttgtgttgtTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAt                          >  2:581697/1‑93 (MQ=255)

                                                                gttgtgttgtTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAt                          >  2:2187882/1‑93 (MQ=255)

                                                                 ttgtgttgtTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATc                         >  2:4591840/1‑93 (MQ=255)

                                                                 ttgtgttgtTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATc                         <  1:3169914/93‑1 (MQ=255)

                                                                  tgtgttgtTGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCg                        >  1:4556528/1‑93 (MQ=255)

                                                                     gttgttGCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCt                     >  2:1938279/1‑93 (MQ=255)

                                                                       tgttgCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCa                   >  1:2157273/1‑93 (MQ=255)

                                                                        gttgCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCAt                  <  1:4217184/93‑1 (MQ=255)

                                                                         ttgCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATc                 <  1:176904/93‑1 (MQ=255)

                                                                           gCCATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtt               >  2:321076/1‑93 (MQ=255)

                                                                             cATGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtttt             >  2:4447912/1‑93 (MQ=255)

                                                                              aTGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTa            <  1:1551288/93‑1 (MQ=255)

                                                                               tGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCttttct           >  2:819926/1‑91 (MQ=255)

                                                                               tGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           <  1:104207/93‑1 (MQ=255)

                                                                               tGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           <  1:1698839/93‑1 (MQ=255)

                                                                               tGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           >  2:4489932/1‑93 (MQ=255)

                                                                               tGGTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt           <  2:4431860/93‑1 (MQ=255)

                                                                                ggTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg          >  2:2307982/1‑93 (MQ=255)

                                                                                ggTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg          <  1:115446/93‑1 (MQ=255)

                                                                                 gTATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGc         <  1:2487098/93‑1 (MQ=255)

                                                                                  tATTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCa        <  2:3319027/93‑1 (MQ=255)

                                                                                   aTTCTCCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAg       >  1:2003773/1‑93 (MQ=255)

                                                                                      ctcCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTcc    <  1:3457340/93‑1 (MQ=255)

                                                                                       tcCGT‑ACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCt   >  2:430453/1‑93 (MQ=255)

GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTG  >  NC_000913/4013679‑4013857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  NC_000913/4013680‑4013865

CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                                                                                <  SRR4302535.4330934/94‑1 (MQ=60)

     TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTAT                                                                                           >  SRR4302535.2424636/1‑94 (MQ=60)

       CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAA                                                                                         >  SRR4302535.2241990/1‑94 (MQ=60)

        GGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAA                                                                                        <  SRR4302535.3413677/94‑1 (MQ=60)

         GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAA                                                                                       <  SRR4302535.750698/94‑1 (MQ=60)

         GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAA                                                                                       >  SRR4302535.334238/1‑94 (MQ=60)

         GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAA                                                                                       <  SRR4302535.1426136/94‑1 (MQ=60)

           GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAAT                                                                                     <  SRR4302535.1427089/94‑1 (MQ=60)

            TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATG                                                                                    <  SRR4302535.3534085/94‑1 (MQ=60)

            TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATG                                                                                    <  SRR4302535.4225449/94‑1 (MQ=60)

             CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGT                                                                                   >  SRR4302535.3768806/1‑94 (MQ=60)

             CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAGGGTATTCTCCGTACCC‑TTATAAAAATGT                                                                                   >  SRR4302535.1684674/1‑94 (MQ=60)

              TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTT                                                                                  <  SRR4302535.1733779/94‑1 (MQ=60)

              TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTT                                                                                  <  SRR4302535.4538012/94‑1 (MQ=60)

               GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTG                                                                                 >  SRR4302535.2078776/1‑94 (MQ=60)

               GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTG                                                                                 <  SRR4302535.2775475/94‑1 (MQ=60)

                   GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCA                                                                             >  SRR4302535.4092662/1‑94 (MQ=60)

                   GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCA                                                                             <  SRR4302535.3835546/94‑1 (MQ=60)

                    ATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAA                                                                            >  SRR4302535.4406098/1‑94 (MQ=60)

                     TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAAT                                                                           >  SRR4302535.35544/1‑94 (MQ=60)

                     TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAAT                                                                           >  SRR4302535.3353539/1‑94 (MQ=60)

                      GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATG                                                                          >  SRR4302535.1744497/1‑94 (MQ=60)

                      GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATG                                                                          <  SRR4302535.1570770/94‑1 (MQ=60)

                        TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTT                                                                        <  SRR4302535.4271662/94‑1 (MQ=60)

                        TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTT                                                                        <  SRR4302535.4687376/94‑1 (MQ=60)

                         CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTA                                                                       >  SRR4302535.3013645/1‑94 (MQ=60)

                         CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTA                                                                       >  SRR4302535.2861584/1‑94 (MQ=60)

                          GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAA                                                                      <  SRR4302535.2295964/94‑1 (MQ=60)

                          GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAA                                                                      <  SRR4302535.1256732/94‑1 (MQ=60)

                           AAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAAC                                                                     <  SRR4302535.1428115/94‑1 (MQ=60)

                            AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACT                                                                    >  SRR4302535.4632524/1‑94 (MQ=60)

                             GCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTA                                                                   <  SRR4302535.126199/94‑1 (MQ=60)

                              CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTAT                                                                  >  SRR4302535.2990862/1‑94 (MQ=60)

                              CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTAT                                                                  <  SRR4302535.599325/94‑1 (MQ=60)

                              CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTAT                                                                  <  SRR4302535.3080304/94‑1 (MQ=60)

                               GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA                                                                 >  SRR4302535.2612376/1‑94 (MQ=60)

                               GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA                                                                 <  SRR4302535.3987302/94‑1 (MQ=60)

                               GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA                                                                 >  SRR4302535.4684736/1‑94 (MQ=60)

                               GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA                                                                 <  SRR4302535.3489082/94‑1 (MQ=60)

                               GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA                                                                 <  SRR4302535.1261753/94‑1 (MQ=60)

                                AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAG                                                                >  SRR4302535.259443/1‑94 (MQ=60)

                                  AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              >  SRR4302535.730196/1‑94 (MQ=60)

                                   GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302535.2142145/94‑1 (MQ=60)

                                   GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             >  SRR4302535.675612/1‑94 (MQ=60)

                                   GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302535.1673703/94‑1 (MQ=60)

                                     cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           >  SRR4302535.4489937/3‑94 (MQ=60)

                                     cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           >  SRR4302535.3458631/3‑94 (MQ=60)

                                     cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           >  SRR4302535.324065/3‑94 (MQ=60)

                                       ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGCATTCTCCGTACCC‑TTATAAAAATGTTGCGCACTGTTACCTATAGTCAGCAT                                                         >  SRR4302535.673891/1‑94 (MQ=60)

                                        TGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATG                                                        >  SRR4302535.3925900/1‑94 (MQ=60)

                                        TGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATG                                                        <  SRR4302535.4661608/94‑1 (MQ=60)

                                          CAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCA                                                      >  SRR4302535.4324400/1‑94 (MQ=60)

                                          CAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCA                                                      >  SRR4302535.3873746/1‑94 (MQ=60)

                                          CAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCA                                                      >  SRR4302535.1862621/1‑94 (MQ=60)

                                          CAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCA                                                      >  SRR4302535.3706809/1‑94 (MQ=60)

                                            GCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAAC                                                    >  SRR4302535.2045618/1‑94 (MQ=60)

                                             CAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATTCAACA                                                   <  SRR4302535.1837611/94‑1 (MQ=60)

                                              AGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAA                                                  >  SRR4302535.4394156/1‑94 (MQ=60)

                                                GTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAT                                                <  SRR4302535.4096393/94‑1 (MQ=60)

                                                GTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAT                                                <  SRR4302535.1836798/94‑1 (MQ=60)

                                                GTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTTATAAAAACGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAT                                                <  SRR4302535.188303/94‑1 (MQ=60)

                                                 TGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATC                                               >  SRR4302535.2148463/1‑94 (MQ=60)

                                                 TGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATC                                               <  SRR4302535.2625722/94‑1 (MQ=60)

                                                 TGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATC                                               <  SRR4302535.4106259/94‑1 (MQ=60)

                                                  GCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCA                                              <  SRR4302535.2878165/94‑1 (MQ=60)

                                                  GCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCA                                              <  SRR4302535.4189318/94‑1 (MQ=60)

                                                  GCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCA                                              <  SRR4302535.1431732/94‑1 (MQ=60)

                                                   CAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCAC                                             <  SRR4302535.68960/94‑1 (MQ=60)

                                                   CAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCAC                                             <  SRR4302535.3753599/94‑1 (MQ=60)

                                                     AATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAT                                           >  SRR4302535.4528720/1‑94 (MQ=60)

                                                     AATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAT                                           >  SRR4302535.1873396/1‑94 (MQ=60)

                                                      ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          >  SRR4302535.1036432/1‑94 (MQ=60)

                                                      ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          <  SRR4302535.2347086/94‑1 (MQ=60)

                                                      ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          <  SRR4302535.1517233/94‑1 (MQ=60)

                                                      ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          >  SRR4302535.1702228/1‑94 (MQ=60)

                                                      ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          >  SRR4302535.1757887/1‑94 (MQ=60)

                                                       TCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTTACTATAGTCAGCATGCAACAAATCACATTG                                         <  SRR4302535.1993539/94‑1 (MQ=60)

                                                       TCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTG                                         >  SRR4302535.3474146/1‑94 (MQ=60)

                                                       TCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTG                                         <  SRR4302535.3592530/94‑1 (MQ=60)

                                                        CCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGC                                        >  SRR4302535.850685/1‑94 (MQ=60)

                                                           GATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     <  SRR4302535.2590374/94‑1 (MQ=60)

                                                            ATTGTTGTGTTGTTGCCATTGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGA                                    >  SRR4302535.4100310/1‑94 (MQ=60)

                                                            ATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGA                                    >  SRR4302535.1708408/1‑94 (MQ=60)

                                                            ATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGA                                    >  SRR4302535.1127070/1‑94 (MQ=60)

                                                              TGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAT                                  >  SRR4302535.3145928/1‑94 (MQ=60)

                                                                TTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                >  SRR4302535.4587643/1‑94 (MQ=60)

                                                                TTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302535.3191868/94‑1 (MQ=60)

                                                                TTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302535.3771538/94‑1 (MQ=60)

                                                                  GTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGC                              >  SRR4302535.1233056/1‑94 (MQ=60)

                                                                     TTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCA                           >  SRR4302535.2172780/1‑94 (MQ=60)

                                                                      TGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCAT                          >  SRR4302535.2653002/1‑94 (MQ=60)

                                                                      TGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCAT                          >  SRR4302535.840023/1‑94 (MQ=60)

                                                                       GTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATC                         <  SRR4302535.4246041/94‑1 (MQ=60)

                                                                        TTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCT                        <  SRR4302535.178133/94‑1 (MQ=60)

                                                                          GCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTT                      >  SRR4302535.2217305/1‑94 (MQ=60)

                                                                           CCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTT                     >  SRR4302535.1507562/1‑94 (MQ=60)

                                                                            CATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    >  SRR4302535.746022/1‑94 (MQ=60)

                                                                            CATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    >  SRR4302535.2356475/1‑94 (MQ=60)

                                                                             ATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAT                   >  SRR4302535.2513719/1‑94 (MQ=60)

                                                                             ATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAT                   <  SRR4302535.1562458/94‑1 (MQ=60)

                                                                             ATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAT                   <  SRR4302535.3651291/94‑1 (MQ=60)

                                                                              TGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATG                  <  SRR4302535.1711117/94‑1 (MQ=60)

                                                                              TGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATG                  <  SRR4302535.104950/94‑1 (MQ=60)

                                                                               GGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGC                 >  SRR4302535.2101456/1‑94 (MQ=60)

                                                                               GGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGC                 <  SRR4302535.116257/94‑1 (MQ=60)

                                                                                GTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCA                >  SRR4302535.3741/1‑94 (MQ=60)

                                                                                GTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCA                >  SRR4302535.3966159/1‑94 (MQ=60)

                                                                                GTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCA                >  SRR4302535.4550111/1‑94 (MQ=60)

                                                                                GTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCA                >  SRR4302535.2306727/1‑94 (MQ=60)

                                                                                GTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCA                <  SRR4302535.2505033/94‑1 (MQ=60)

                                                                                 TATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAG               >  SRR4302535.2018212/1‑94 (MQ=60)

                                                                                     CTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           <  SRR4302535.3481375/94‑1 (MQ=60)

                                                                                     CTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           <  SRR4302535.4190322/94‑1 (MQ=60)

                                                                                       CCGTACCC‑TTATAAAAATGTTGCGCATTGTTAACTATAGTCAGGATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGC         <  SRR4302535.103828/94‑1 (MQ=60)

                                                                                         GTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAG       >  SRR4302535.3511401/1‑94 (MQ=60)

                                                                                          tacCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGA      >  SRR4302535.1298724/4‑94 (MQ=60)

                                                                                           acCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302535.167269/3‑94 (MQ=60)

                                                                                           C‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302535.275517/94‑1 (MQ=60)

                                                                                           C‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302535.1734165/94‑1 (MQ=60)

                                                                                             CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   <  SRR4302535.82025/94‑1 (MQ=60)

                                                                                             CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   >  SRR4302535.4397543/1‑94 (MQ=60)

                                                                                             CC‑TTATAAAAATGATGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   <  SRR4302535.1347780/94‑1 (MQ=60)

                                                                                              C‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302535.4156434/1‑94 (MQ=60)

                                                                                              C‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  <  SRR4302535.2407072/94‑1 (MQ=60)

                                                                                              C‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302535.225721/1‑94 (MQ=60)

                                                                                              C‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302535.700175/1‑94 (MQ=60)

                                                                                              C‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302535.3363925/1‑94 (MQ=60)

                                                                                              C‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302535.760702/1‑94 (MQ=60)

CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  NC_000913/4013680‑4013865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`