Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,041,927	A→G	Y50C (TAC→TGC)	b3861 →	putative GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,041,927	0	A	G	100.0%	527.0 / NA	164	Y50C (TAC→TGC)	b3861	putative GTP‑binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (103/61); total (103/61)

Only 100 of 164 total aligned reads displayed.

ACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCC  >  NC_000913/4041839‑4042015

acaTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGat                                                                                      >  2:754135/1‑93 (MQ=255)

  aTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGatat                                                                                    >  2:4084785/1‑93 (MQ=255)

  aTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGatat                                                                                    >  1:307893/1‑93 (MQ=255)

  aTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGatat                                                                                    <  2:3797623/93‑1 (MQ=255)

   tGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATg                                                                                   >  1:2293177/1‑93 (MQ=255)

    ggTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGACCATGCCTGCGATATGa                                                                                  >  1:4390291/1‑93 (MQ=255)

    ggTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGa                                                                                  >  1:3829376/1‑93 (MQ=255)

    ggTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGa                                                                                  >  1:3233961/1‑93 (MQ=255)

         acaGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTa                                                                             >  1:2872917/1‑93 (MQ=255)

          caGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTaa                                                                            <  2:1341627/93‑1 (MQ=255)

            ggTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGt                                                                          >  2:1953191/1‑93 (MQ=255)

            ggTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGt                                                                          <  1:3923833/93‑1 (MQ=255)

                cGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATc                                                                      <  2:1490108/93‑1 (MQ=255)

                cGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATc                                                                      >  1:3763992/1‑93 (MQ=255)

                  cAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCaa                                                                    <  2:4148538/93‑1 (MQ=255)

                    aaTTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCaaaa                                                                  <  1:168306/93‑1 (MQ=255)

                    aaTTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCaaaa                                                                  >  1:2903564/1‑93 (MQ=255)

                     aTTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAAt                                                                 <  1:4389682/93‑1 (MQ=255)

                         aGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTg                                                             >  2:4196371/1‑93 (MQ=255)

                         aGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTg                                                             >  2:4590183/1‑93 (MQ=255)

                          gCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGa                                                            <  1:2682785/93‑1 (MQ=255)

                          gCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGa                                                            <  2:480283/93‑1 (MQ=255)

                          gCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGa                                                            >  1:2349359/1‑93 (MQ=255)

                            cTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACa                                                          >  1:1528089/1‑93 (MQ=255)

                            cTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACa                                                          >  1:4333169/1‑93 (MQ=255)

                             tGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACaa                                                         >  2:2239181/1‑93 (MQ=255)

                             tGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACaa                                                         >  2:3424452/1‑93 (MQ=255)

                               gtgtCGAGAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAg                                                       >  1:1321664/1‑93 (MQ=255)

                               gtgtCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAg                                                       <  1:1813128/93‑1 (MQ=255)

                                tgtCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAgg                                                      >  2:195961/1‑93 (MQ=255)

                                 gtCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGc                                                     >  2:3510488/1‑93 (MQ=255)

                                  tCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCt                                                    >  1:2084966/1‑93 (MQ=255)

                                  tCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTT‑ACAAAAGcct                                                   >  1:2731571/1‑91 (MQ=255)

                                   cGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCtt                                                   <  1:4493543/93‑1 (MQ=255)

                                    gAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCttt                                                  <  1:951837/93‑1 (MQ=255)

                                     aaaaaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCtttt                                                 >  2:2315563/1‑93 (MQ=255)

                                      aacaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTCAGTAATCAAAATTTTGACAAAGGCttttt                                                <  1:1812296/90‑1 (MQ=255)

                                      aaaaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCttttt                                                >  1:3048436/1‑93 (MQ=255)

                                      aaaaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCttttt                                                >  2:1176119/1‑93 (MQ=255)

                                        aaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTa                                              >  1:1070136/1‑93 (MQ=255)

                                         aTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTaa                                             <  1:3457923/93‑1 (MQ=255)

                                          tttATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAAt                                            <  2:2116142/93‑1 (MQ=255)

                                          tttATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAAt                                            >  1:3272987/1‑93 (MQ=255)

                                          tttATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAAt                                            <  2:42578/93‑1 (MQ=255)

                                            tattaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTc                                          >  2:2947917/1‑93 (MQ=255)

                                            tattaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTc                                          >  2:2599417/1‑93 (MQ=255)

                                              ttaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTccc                                        <  2:1120295/93‑1 (MQ=255)

                                               taAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCg                                       >  1:4527677/1‑93 (MQ=255)

                                                  aGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGttt                                    <  1:3829709/93‑1 (MQ=255)

                                                   gATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGtttt                                   >  1:3698032/1‑93 (MQ=255)

                                                   gATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGtgtt                                   >  2:1585850/1‑93 (MQ=255)

                                                     ttttAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTca                                 >  1:1283591/1‑93 (MQ=255)

                                                      tttAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaa                                >  2:621266/1‑93 (MQ=255)

                                                      tttAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaa                                <  2:3917506/93‑1 (MQ=255)

                                                       ttAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaa                               <  2:4530833/93‑1 (MQ=255)

                                                       ttAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaa                               <  2:3386574/93‑1 (MQ=255)

                                                       ttAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaa                               >  1:4560867/1‑93 (MQ=255)

                                                       ttAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaa                               >  1:3714713/1‑93 (MQ=255)

                                                       ttAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaa                               <  2:1427024/93‑1 (MQ=255)

                                                         aaCGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatg                             >  2:2586813/1‑93 (MQ=255)

                                                          aCGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatgc                            >  1:767320/1‑93 (MQ=255)

                                                            gATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatgcaa                          >  1:983316/1‑93 (MQ=255)

                                                            gATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatgcaa                          >  1:3752281/1‑93 (MQ=255)

                                                             aTGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatgcaaa                         >  1:2097086/1‑93 (MQ=255)

                                                              tGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatgcaaat                        <  2:3914075/93‑1 (MQ=255)

                                                              tGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatgcaaat                        >  2:2027497/1‑93 (MQ=255)

                                                              tGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatgcaaat                        <  2:1277777/93‑1 (MQ=255)

                                                              tGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTcaaatgcaaat                        >  1:412109/1‑93 (MQ=255)

                                                                cTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGa                      <  1:1423289/93‑1 (MQ=255)

                                                                 tAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGAc                     >  1:544363/1‑93 (MQ=255)

                                                                 tAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGAc                     >  2:3894343/1‑93 (MQ=255)

                                                                 tAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGAc                     >  2:4128530/1‑93 (MQ=255)

                                                                 tAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGAc                     >  2:1678266/1‑93 (MQ=255)

                                                                 tAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGATTTTTTAATTCCCGTTTTCAAATGCAAATGAc                     >  1:112133/1‑93 (MQ=255)

                                                                  aaaaaGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACa                    <  1:3562610/93‑1 (MQ=255)

                                                                   aaaaGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACAt                   <  1:1510022/93‑1 (MQ=255)

                                                                   aaaaGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACAt                   >  2:136639/1‑93 (MQ=255)

                                                                    aaagaaagGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACAtt                  >  1:1983089/1‑93 (MQ=255)

                                                                      agaaagGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCg                >  1:3608556/1‑93 (MQ=255)

                                                                       gaaagGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGGTTTCAAATGCAAATGACATTCGa               <  2:1560984/93‑1 (MQ=255)

                                                                       aaaagGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGa               <  2:3747421/92‑1 (MQ=255)

                                                                         aagGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATa             >  2:4347204/1‑93 (MQ=255)

                                                                          agGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCGAATGCAAATGACATTCGATaa            >  2:167512/1‑93 (MQ=255)

                                                                          agGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATaa            >  2:908052/1‑93 (MQ=255)

                                                                          agGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATaa            >  1:4618500/1‑93 (MQ=255)

                                                                          agGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATaa            <  2:3750701/93‑1 (MQ=255)

                                                                           ggTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAAc           <  2:244163/93‑1 (MQ=255)

                                                                           ggTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAAc           >  1:2496118/1‑93 (MQ=255)

                                                                           ggTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAAc           <  1:2422680/93‑1 (MQ=255)

                                                                            gTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACg          <  2:2997203/93‑1 (MQ=255)

                                                                            gTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACg          <  2:3190716/93‑1 (MQ=255)

                                                                             tGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACgg         >  2:4084386/1‑93 (MQ=255)

                                                                               aaCATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTg       >  2:1985227/1‑93 (MQ=255)

                                                                               aaCATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTg       <  1:2525188/93‑1 (MQ=255)

                                                                                aCATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGc      <  2:1917170/93‑1 (MQ=255)

                                                                                aCATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATCCAAATGACATTCGATAACGGTGc      >  2:747776/1‑93 (MQ=255)

                                                                                 cATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCa     <  1:522552/93‑1 (MQ=255)

                                                                                  aTGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATTACATTCGATAACGGTGCAc    >  2:3632592/1‑93 (MQ=255)

                                                                                   tGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAcc   >  2:3393017/1‑93 (MQ=255)

                                                                                    gCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAccc  <  2:2730132/93‑1 (MQ=255)

ACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCC  >  NC_000913/4041839‑4042015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAC  >  NC_000913/4041840‑4042013

CATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATAT                                                                                  >  SRR4302535.310024/1‑94 (MQ=60)

 ATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATG                                                                                 >  SRR4302535.2309582/1‑94 (MQ=60)

 ATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATG                                                                                 >  SRR4302535.1908506/1‑94 (MQ=60)

  TGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGACCATGCCTGCGATATGA                                                                                >  SRR4302535.4420330/1‑94 (MQ=60)

  TGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGA                                                                                >  SRR4302535.3855787/1‑94 (MQ=60)

  TGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGA                                                                                >  SRR4302535.3256402/1‑94 (MQ=60)

   GGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGAC                                                                               >  SRR4302535.4519885/1‑94 (MQ=60)

       CACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTA                                                                           >  SRR4302535.2893274/1‑94 (MQ=60)

          AGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGT                                                                        >  SRR4302535.2242150/1‑94 (MQ=60)

           GGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTT                                                                       <  SRR4302535.3950647/94‑1 (MQ=60)

             TACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTAT                                                                     <  SRR4302535.4601406/94‑1 (MQ=60)

              ACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATC                                                                    >  SRR4302535.3789995/1‑94 (MQ=60)

                  AAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAA                                                                >  SRR4302535.2924087/1‑94 (MQ=60)

                  AAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAA                                                                >  SRR4302535.2924086/1‑94 (MQ=60)

                   AATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAAT                                                               <  SRR4302535.169474/94‑1 (MQ=60)

                    ATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATT                                                              <  SRR4302535.4419716/94‑1 (MQ=60)

                        AGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGA                                                          >  SRR4302535.2366205/1‑94 (MQ=60)

                        AGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGA                                                          >  SRR4302535.3912610/1‑94 (MQ=60)

                         GCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGAC                                                         <  SRR4302535.2701974/94‑1 (MQ=60)

                          CCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACA                                                        >  SRR4302535.4362804/1‑94 (MQ=60)

                          CCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACA                                                        >  SRR4302535.1539095/1‑94 (MQ=60)

                          CCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACA                                                        >  SRR4302535.3283378/1‑94 (MQ=60)

                             GGTGTCGAGAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAG                                                     >  SRR4302535.1331099/1‑94 (MQ=60)

                             GGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAG                                                     <  SRR4302535.202051/94‑1 (MQ=60)

                              GTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGG                                                    <  SRR4302535.1826239/94‑1 (MQ=60)

                              GTGTCGAAAAATTTATTAAAGATTTTAACGATGATAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGG                                                    <  SRR4302535.778112/94‑1 (MQ=60)

                                GTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTacaaaagcct                                                  >  SRR4302535.2751071/1‑84 (MQ=60)

                                GTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCT                                                  >  SRR4302535.2099982/1‑94 (MQ=60)

                                GTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCT                                                  >  SRR4302535.1404582/1‑94 (MQ=60)

                                 TCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTT                                                 <  SRR4302535.4399236/94‑1 (MQ=60)

                                  CGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTT                                                <  SRR4302535.4524386/94‑1 (MQ=60)

                                   tgattgtttctttgtttttttttttttttttttttcaagcagaagacggcaTACGAGATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTT                                               <  SRR4302535.3035668/43‑1 (MQ=60)

                                   GAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTT                                               <  SRR4302535.958585/94‑1 (MQ=60)

                                    AAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTT                                              >  SRR4302535.3069790/1‑94 (MQ=60)

                                     AACATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTCAGTAATCAAAATTTTGACAAAGGCTTTTTT                                             <  SRR4302535.1825403/94‑1 (MQ=60)

                                      AAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTA                                            >  SRR4302535.1077743/1‑94 (MQ=60)

                                       AATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAA                                           >  SRR4302535.3812895/1‑94 (MQ=60)

                                        ATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAAT                                          >  SRR4302535.3295721/1‑94 (MQ=60)

                                        ATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAAT                                          <  SRR4302535.3481964/94‑1 (MQ=60)

                                         TTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATT                                         <  SRR4302535.3692840/94‑1 (MQ=60)

                                            ATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCC                                      >  SRR4302535.2510506/1‑94 (MQ=60)

                                             TTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCG                                     >  SRR4302535.4558687/1‑94 (MQ=60)

                                              TAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGT                                    >  SRR4302535.3189841/1‑94 (MQ=60)

                                              TAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGT                                    >  SRR4302535.2406331/1‑94 (MQ=60)

                                              TAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGT                                    >  SRR4302535.4542545/1‑94 (MQ=60)

                                               AAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTT                                   >  SRR4302535.1596483/1‑94 (MQ=60)

                                               AAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTT                                   >  SRR4302535.1189175/1‑94 (MQ=60)

                                                AAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTT                                  <  SRR4302535.2955320/94‑1 (MQ=60)

                                                 AGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTT                                 >  SRR4302535.3723649/1‑94 (MQ=60)

                                                 AGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTT                                 <  SRR4302535.3856122/94‑1 (MQ=60)

                                                  GATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTC                                >  SRR4302535.3727700/1‑94 (MQ=60)

                                                   ATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCA                               >  SRR4302535.597287/1‑94 (MQ=60)

                                                   ATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCA                               >  SRR4302535.1292742/1‑94 (MQ=60)

                                                     TTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAA                             <  SRR4302535.2660192/94‑1 (MQ=60)

                                                     TTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAA                             >  SRR4302535.3740447/1‑94 (MQ=60)

                                                     TTTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAA                             >  SRR4302535.4591997/1‑94 (MQ=60)

                                                      TTAACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAAT                            <  SRR4302535.4671789/94‑1 (MQ=60)

                                                        AACGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGC                          >  SRR4302535.772723/1‑94 (MQ=60)

                                                          CGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAA                        >  SRR4302535.990300/1‑94 (MQ=60)

                                                          CGATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAA                        >  SRR4302535.3778250/1‑94 (MQ=60)

                                                           GATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAA                       >  SRR4302535.2112208/1‑94 (MQ=60)

                                                            ATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAAT                      >  SRR4302535.415006/1‑94 (MQ=60)

                                                            ATGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAAT                      <  SRR4302535.1775342/94‑1 (MQ=60)

                                                             TGCTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATG                     <  SRR4302535.3780212/94‑1 (MQ=60)

                                                               CTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGAC                   <  SRR4302535.1433452/94‑1 (MQ=60)

                                                               CTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGAC                   >  SRR4302535.548167/1‑94 (MQ=60)

                                                               CTAAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGATTTTTTAATTCCCGTTTTCAAATGCAAATGAC                   >  SRR4302535.112921/1‑94 (MQ=60)

                                                                 AAAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACAT                 <  SRR4302535.3587311/94‑1 (MQ=60)

                                                                  AAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATT                >  SRR4302535.1997368/1‑94 (MQ=60)

                                                                  AAAAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATT                <  SRR4302535.1520903/94‑1 (MQ=60)

                                                                    AAGAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCG              >  SRR4302535.3633580/1‑94 (MQ=60)

                                                                     AGAAAGGTGAACATGCCTGCGATATGACGTCAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGA             >  SRR4302535.271659/1‑94 (MQ=60)

                                                                      GAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGAT            >  SRR4302535.1512159/1‑94 (MQ=60)

                                                                      GAAAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGAT            <  SRR4302535.4093881/94‑1 (MQ=60)

                                                                        AAGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAA          >  SRR4302535.4649884/1‑94 (MQ=60)

                                                                         AGGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAAC         >  SRR4302535.2514125/1‑94 (MQ=60)

                                                                          GGTGAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACG        <  SRR4302535.2440134/94‑1 (MQ=60)

                                                                             GAACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTG     >  SRR4302535.3482805/1‑94 (MQ=60)

                                                                              AACATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGC    <  SRR4302535.2543384/94‑1 (MQ=60)

                                                                                CATGCCTGCGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAC  <  SRR4302535.526207/94‑1 (MQ=60)

CATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAC  >  NC_000913/4041840‑4042013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`