Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,083,471 A→G intergenic (‑69/‑125) fdoG ← / → fdhD formate dehydrogenase‑O, major subunit/affects formate dehydrogenase‑N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,083,4710AG99.6% 798.3 / ‑5.6 253intergenic (‑69/‑125)fdoG/fdhDformate dehydrogenase‑O, major subunit/affects formate dehydrogenase‑N
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (133/119);  minor base T (0/1);  total (133/120)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 280 total aligned reads displayed.

ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA  >  NC_000913/4083381‑4083560
                                                                                          |                                                                                         
 cTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGtt                                                                                        >  2:2813861/1‑93 (MQ=255)
  tGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTa                                                                                       <  1:2261161/93‑1 (MQ=255)
    ccTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtt                                                                                     >  1:2704857/1‑93 (MQ=255)
    ccTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtt                                                                                     >  1:2534149/1‑93 (MQ=255)
    ccTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtt                                                                                     <  2:198484/93‑1 (MQ=255)
    ccTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtt                                                                                     >  2:835603/1‑93 (MQ=255)
     cTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAttt                                                                                    >  2:3352636/1‑93 (MQ=255)
      ttCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtttt                                                                                   >  1:3646586/1‑93 (MQ=255)
        ctgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTc                                                                                 <  2:3447143/93‑1 (MQ=255)
         tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCt                                                                                <  1:1694601/93‑1 (MQ=255)
         tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCt                                                                                >  2:932418/1‑93 (MQ=255)
          gctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCtt                                                                               >  2:1860144/1‑93 (MQ=255)
           ctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCttt                                                                              >  1:492494/1‑93 (MQ=255)
            tgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTg                                                                             <  2:3880235/93‑1 (MQ=255)
            tgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGGTATTTTTCTTTg                                                                             >  2:1361121/1‑93 (MQ=255)
              aCCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCg                                                                           >  1:1505418/1‑93 (MQ=255)
                 tGCATGGATTGCTCCTTGTTTCGACATTCTCACGTCCCATTTACATTCGCTTGCTGCGGGTGCAGGGAGTGGGGGGTATTTTTCTTTGCggga                                                                        >  2:462190/1‑91 (MQ=255)
                   cATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgg                                                                      <  2:4365871/93‑1 (MQ=255)
                   cATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgg                                                                      >  2:127101/1‑93 (MQ=255)
                    aTGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAggg                                                                     <  1:1105252/93‑1 (MQ=255)
                      ggATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGc                                                                   >  1:4179301/1‑93 (MQ=255)
                      ggATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGc                                                                   >  2:2443876/1‑93 (MQ=255)
                       gATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAggggtc                                                                  >  2:2545938/1‑91 (MQ=255)
                       gATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGcc                                                                  <  2:903626/93‑1 (MQ=255)
                       gATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGcc                                                                  >  2:4264215/1‑93 (MQ=255)
                          tGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCa                                                               >  2:2151471/1‑93 (MQ=255)
                           gCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaa                                                              >  2:4565049/1‑93 (MQ=255)
                           gCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGCTATTTTTCTTTGCGGAAGGAGCCGCaa                                                              >  1:799566/1‑93 (MQ=255)
                           gCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGAGGGGGTTATTTTTCTTTGCGGAAGCGGCCGcga                                                              >  2:1122175/1‑91 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             >  1:2337220/1‑93 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             <  1:329675/93‑1 (MQ=255)
                             tCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAg                                                            >  2:1691231/1‑93 (MQ=255)
                             tCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAg                                                            <  2:3546605/93‑1 (MQ=255)
                             tCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGGTATTTTTCTTTGCGGAAGGGGCCGCAAAg                                                            >  1:4587695/1‑93 (MQ=255)
                                  gTTTCGACATTGTCACGTCCCATTCACGTTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCa                                                       <  2:4170672/93‑1 (MQ=255)
                                   tttCGACATTGTCACGTCCCATTTACATTCGCTGGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAg                                                      <  1:2623435/93‑1 (MQ=255)
                                     tCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGaa                                                    <  1:1819552/93‑1 (MQ=255)
                                     tCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGaa                                                    <  2:2184467/93‑1 (MQ=255)
                                       gACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAtt                                                  >  2:492298/1‑93 (MQ=255)
                                       gACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAtt                                                  >  2:4407573/1‑93 (MQ=255)
                                        aCATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTg                                                 >  2:3997489/1‑93 (MQ=255)
                                         cATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTgg                                                >  1:3196767/1‑93 (MQ=255)
                                          aTTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGc                                               >  2:1798279/1‑93 (MQ=255)
                                          aTTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGGTATTTTTCTTTGCGGGAAGGGCCGCCAAGGTACAGAATTGGc                                               >  2:3118917/1‑93 (MQ=255)
                                           ttGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCt                                              >  2:1019877/1‑93 (MQ=255)
                                            tGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTc                                             <  2:3012567/93‑1 (MQ=255)
                                             gTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCa                                            >  1:4479706/1‑93 (MQ=255)
                                              tCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCaa                                           <  2:386576/93‑1 (MQ=255)
                                              tCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCaa                                           <  1:2993270/93‑1 (MQ=255)
                                               cACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAt                                          <  1:3989308/93‑1 (MQ=255)
                                                aCGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAtt                                         >  2:4096040/1‑93 (MQ=255)
                                                aCGTCCCATTTACATTCGCGTGCTGCGTGTGCAGGGAGGGGGTGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCTAGAATTGGCTCAAtt                                         <  2:764908/93‑1 (MQ=255)
                                                  gTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTc                                       <  2:4424464/93‑1 (MQ=255)
                                                  gTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTc                                       <  1:3855989/93‑1 (MQ=255)
                                                   tCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTcc                                      >  1:2979353/1‑93 (MQ=255)
                                                   tCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTcc                                      >  1:4533455/1‑93 (MQ=255)
                                                    cccATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTccc                                     <  1:4120810/93‑1 (MQ=255)
                                                     ccATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCt                                    >  1:1946384/1‑93 (MQ=255)
                                                      cATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtc                                   >  1:2142582/1‑93 (MQ=255)
                                                      cATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtc                                   >  2:2270352/1‑93 (MQ=255)
                                                      cATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtc                                   >  2:2359034/1‑93 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 <  2:4591146/93‑1 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 <  1:1375982/93‑1 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 <  2:3798752/93‑1 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 >  1:3368049/1‑93 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 <  1:2649535/93‑1 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 >  2:2884466/1‑93 (MQ=255)
                                                          tACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatc                               >  2:1827124/1‑93 (MQ=255)
                                                             aTTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAg                            >  1:3290000/1‑93 (MQ=255)
                                                             aTTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAg                            <  2:3244482/93‑1 (MQ=255)
                                                                cGCTTGCTGCGTGTGCAGGGAGTGGTGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGaa                         <  1:1347435/93‑1 (MQ=255)
                                                                cGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGaa                         <  1:3474024/93‑1 (MQ=255)
                                                                 gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        <  1:4139722/93‑1 (MQ=255)
                                                                 gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        <  2:4103903/93‑1 (MQ=255)
                                                                 gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        <  2:2109706/93‑1 (MQ=255)
                                                                  cTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAgg                       <  2:543079/93‑1 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      >  1:3920943/1‑93 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      <  1:738697/93‑1 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      >  1:2809311/1‑93 (MQ=255)
                                                                    tgctgcGTGTGCAGGGAGTGTGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGt                     <  1:2333461/93‑1 (MQ=255)
                                                                     gctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGtt                    >  2:3674870/1‑93 (MQ=255)
                                                                       tgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTg                  <  2:2522664/93‑1 (MQ=255)
                                                                           tgtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              >  1:1233642/1‑93 (MQ=255)
                                                                           tgtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              >  1:1053132/1‑93 (MQ=255)
                                                                           tgtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              <  2:3062119/93‑1 (MQ=255)
                                                                           tgtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              >  1:2035947/1‑93 (MQ=255)
                                                                           tgtgCAGGGAGTGCGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              <  2:1507082/93‑1 (MQ=255)
                                                                           tgtgCAGGGAGGGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTTCCTCCATCCAGGAAGGGTTTGTaaa              >  1:4186803/1‑92 (MQ=255)
                                                                             tgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAg            >  1:158313/1‑93 (MQ=255)
                                                                              gCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGa           <  2:777245/93‑1 (MQ=255)
                                                                              gCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGa           <  1:1138991/93‑1 (MQ=255)
                                                                               cAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGaa          <  2:2922712/93‑1 (MQ=255)
                                                                                 gggagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATa        >  1:893922/1‑93 (MQ=255)
                                                                                 gggagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATa        <  1:4503681/93‑1 (MQ=255)
                                                                                  ggagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAc       <  2:1339373/93‑1 (MQ=255)
                                                                                   gagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAcc      <  2:1650720/93‑1 (MQ=255)
                                                                                   gagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAcc      <  1:2923609/93‑1 (MQ=255)
                                                                                    agtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCa     >  2:3428008/1‑93 (MQ=255)
                                                                                       gggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATaa  <  1:669667/93‑1 (MQ=255)
                                                                                       gggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATaa  <  1:576775/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA  >  NC_000913/4083381‑4083560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  NC_000913/4083378‑4083564
                                                                                             |                                                                                             
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGG                                                                                               >  SRR4302535.3858118/1‑94 (MQ=60)
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGG                                                                                               <  SRR4302535.708445/94‑1 (MQ=60)
 GAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGG                                                                                              >  SRR4302535.734436/1‑94 (MQ=60)
  AACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGT                                                                                             >  SRR4302535.3362399/1‑94 (MQ=60)
  AACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGT                                                                                             >  SRR4302535.3160291/1‑94 (MQ=60)
   ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTT                                                                                            >  SRR4302535.805220/1‑94 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTA                                                                                           >  SRR4302535.1202403/1‑94 (MQ=60)
     TGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAT                                                                                          <  SRR4302535.2277268/94‑1 (MQ=60)
      GCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATT                                                                                         >  SRR4302535.2724186/1‑94 (MQ=60)
      GCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATT                                                                                         >  SRR4302535.2552401/1‑94 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTT                                                                                        >  SRR4302535.3013117/1‑94 (MQ=60)
        CTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       >  SRR4302535.3099850/1‑94 (MQ=60)
        CTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       >  SRR4302535.1360136/1‑94 (MQ=60)
        CTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       >  SRR4302535.3671838/1‑94 (MQ=60)
        CGTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       <  SRR4302535.1963134/94‑1 (MQ=60)
         TTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTT                                                                                      >  SRR4302535.3315160/1‑94 (MQ=60)
         TTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTT                                                                                      <  SRR4302535.4246034/94‑1 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302535.2683306/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    <  SRR4302535.509219/94‑1 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    <  SRR4302535.1131965/94‑1 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   >  SRR4302535.1110082/1‑94 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   <  SRR4302535.1706854/94‑1 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   <  SRR4302535.4448139/94‑1 (MQ=60)
             GCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTT                                                                                  >  SRR4302535.1932741/1‑94 (MQ=60)
             GCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTT                                                                                  >  SRR4302535.495946/1‑94 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 >  SRR4302535.3562940/1‑94 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 <  SRR4302535.3248468/94‑1 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 >  SRR4302535.4129163/1‑94 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 <  SRR4302535.686037/94‑1 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 >  SRR4302535.3955685/1‑94 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                >  SRR4302535.2196763/1‑94 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302535.2281157/94‑1 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302535.4011736/94‑1 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               >  SRR4302535.4204291/1‑94 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               >  SRR4302535.1516241/1‑94 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGATATTTTTCTTTGCG                                                                               >  SRR4302535.3002165/1‑94 (MQ=60)
                 ACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGG                                                                              >  SRR4302535.2637574/1‑94 (MQ=60)
                  CCTGCATGTATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGA                                                                             <  SRR4302535.2665498/94‑1 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            <  SRR4302535.4527868/94‑1 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           <  SRR4302535.1616104/94‑1 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302535.1328260/94‑1 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302535.2567033/94‑1 (MQ=60)
                      CATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGG                                                                         <  SRR4302535.3275293/94‑1 (MQ=60)
                       ATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGG                                                                        <  SRR4302535.1113150/94‑1 (MQ=60)
                        TGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGC                                                                       >  SRR4302535.4207906/1‑94 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCC                                                                      >  SRR4302535.2970448/1‑94 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGCGCC                                                                      >  SRR4302535.3634680/1‑94 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCGTTGCGGAAGGGGGC                                                                      >  SRR4302535.1284307/1‑94 (MQ=60)
                           ATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGC                                                                    >  SRR4302535.45598/1‑94 (MQ=60)
                             TGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGCTATTTTTCTTTGCGGAAGGAGCCGCAA                                                                  >  SRR4302535.805180/1‑94 (MQ=60)
                             TGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGCGGGGGTTATTTTTCTTTGCGGAAGGGGACGCAA                                                                  >  SRR4302535.592571/1‑94 (MQ=60)
                              GCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAA                                                                 >  SRR4302535.2353943/1‑94 (MQ=60)
                              GCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAA                                                                 >  SRR4302535.2967970/1‑94 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                <  SRR4302535.331962/94‑1 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                <  SRR4302535.2210100/94‑1 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                <  SRR4302535.4167576/94‑1 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGGTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                >  SRR4302535.4618934/1‑94 (MQ=60)
                                 CCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGT                                                              >  SRR4302535.978927/1‑94 (MQ=60)
                                 CCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGT                                                              <  SRR4302535.1536285/94‑1 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             >  SRR4302535.4590758/1‑94 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             <  SRR4302535.4134471/94‑1 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           <  SRR4302535.698449/94‑1 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           <  SRR4302535.3680405/94‑1 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           <  SRR4302535.2769623/94‑1 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           >  SRR4302535.1365148/1‑94 (MQ=60)
                                     GTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAG                                                          >  SRR4302535.1767940/1‑94 (MQ=60)
                                      TTTCGACATTGTCACGTCCCATTTACATTCGCTGGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGA                                                         <  SRR4302535.2642235/94‑1 (MQ=60)
                                       TTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAA                                                        <  SRR4302535.116232/94‑1 (MQ=60)
                                       TTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAA                                                        <  SRR4302535.1741926/94‑1 (MQ=60)
                                        TCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAT                                                       <  SRR4302535.1832698/94‑1 (MQ=60)
                                        TCGACATTGTCACGTCCCATGTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAT                                                       <  SRR4302535.214401/94‑1 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      >  SRR4302535.3887265/1‑94 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      <  SRR4302535.1698391/94‑1 (MQ=60)
                                           ACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGG                                                    >  SRR4302535.3218887/1‑94 (MQ=60)
                                           ACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGG                                                    >  SRR4302535.3733054/1‑94 (MQ=60)
                                           ACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGG                                                    >  SRR4302535.784687/1‑94 (MQ=60)
                                           ACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGG                                                    <  SRR4302535.3392269/94‑1 (MQ=60)
                                            CATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGC                                                   >  SRR4302535.772367/1‑94 (MQ=60)
                                               TGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCA                                                >  SRR4302535.4510459/1‑94 (MQ=60)
                                               TGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCA                                                >  SRR4302535.4664564/1‑94 (MQ=60)
                                                 TCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAT                                              <  SRR4302535.3014259/94‑1 (MQ=60)
                                                  CACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATT                                             <  SRR4302535.4016592/94‑1 (MQ=60)
                                                    CGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTC                                           <  SRR4302535.2825671/94‑1 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302535.3000256/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302535.4564480/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          <  SRR4302535.3882540/94‑1 (MQ=60)
                                                       CCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCT                                        <  SRR4302535.4149008/94‑1 (MQ=60)
                                                       CCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCT                                        >  SRR4302535.1960394/1‑94 (MQ=60)
                                                        CCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTC                                       >  SRR4302535.2084591/1‑94 (MQ=60)
                                                        CCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTC                                       >  SRR4302535.2157996/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      <  SRR4302535.4613914/94‑1 (MQ=60)
                                                          ATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCA                                     >  SRR4302535.3391484/1‑94 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    >  SRR4302535.1120506/1‑94 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    <  SRR4302535.2668509/94‑1 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    <  SRR4302535.1385808/94‑1 (MQ=60)
                                                              ACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCA                                 >  SRR4302535.581330/1‑94 (MQ=60)
                                                              ACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCA                                 <  SRR4302535.4213801/94‑1 (MQ=60)
                                                               CATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAG                                >  SRR4302535.3312874/1‑94 (MQ=60)
                                                                   CGCTTGCTGCGTGTGCAGGGAGTGGTGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAG                            <  SRR4302535.1357053/94‑1 (MQ=60)
                                                                   CGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAG                            <  SRR4302535.3498160/94‑1 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           <  SRR4302535.2939174/94‑1 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           <  SRR4302535.1569403/94‑1 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           <  SRR4302535.4168069/94‑1 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          >  SRR4302535.2829282/1‑94 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          >  SRR4302535.3947750/1‑94 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          >  SRR4302535.4515719/1‑94 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          <  SRR4302535.3350608/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302535.2646060/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302535.743891/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302535.2293788/94‑1 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGTGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        <  SRR4302535.2350157/94‑1 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        >  SRR4302535.2352682/1‑94 (MQ=60)
                                                                        GCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTT                       <  SRR4302535.3830400/94‑1 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302535.1464173/1‑94 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302535.2699926/1‑94 (MQ=60)
                                                                           GCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGCTTGTA                    >  SRR4302535.2416212/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302535.1242461/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  <  SRR4302535.4178918/94‑1 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302535.2050612/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302535.3427760/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302535.2351023/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302535.1060620/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGGGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTTCCTCCATCCAGGAAGGGTTTGTAAA                  >  SRR4302535.4215456/1‑94 (MQ=60)
                                                                               GTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAG                >  SRR4302535.4545332/1‑94 (MQ=60)
                                                                               GTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAG                >  SRR4302535.159429/1‑94 (MQ=60)
                                                                                 GCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAA              <  SRR4302535.1147110/94‑1 (MQ=60)
                                                                                  CAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAAT             <  SRR4302535.4167394/94‑1 (MQ=60)
                                                                                   AGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATA            <  SRR4302535.502799/94‑1 (MQ=60)
                                                                                   AGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATA            >  SRR4302535.900260/1‑94 (MQ=60)
                                                                                    GGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAC           <  SRR4302535.4534606/94‑1 (MQ=60)
                                                                                      GAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCA         <  SRR4302535.2944226/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        <  SRR4302535.1896481/94‑1 (MQ=60)
                                                                                          GGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAAT     <  SRR4302535.674341/94‑1 (MQ=60)
                                                                                          GGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAAT     <  SRR4302535.580827/94‑1 (MQ=60)
                                                                                          GGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAAT     >  SRR4302535.4049079/1‑94 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    <  SRR4302535.3735096/94‑1 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   >  SRR4302535.978853/1‑94 (MQ=60)
                                                                                             GGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  <  SRR4302535.1093914/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  NC_000913/4083378‑4083564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑