Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,083,471 A→G intergenic (‑69/‑125) fdoG ← / → fdhD formate dehydrogenase‑O, major subunit/affects formate dehydrogenase‑N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,083,4710AG100.0% 1041.2 / NA 328intergenic (‑69/‑125)fdoG/fdhDformate dehydrogenase‑O, major subunit/affects formate dehydrogenase‑N
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (174/154);  total (174/154)

Only 100 of 376 total aligned reads displayed.

ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA  >  NC_000913/4083381‑4083560
                                                                                          |                                                                                         
aCTGCCTTCTGCTGACCTGTATGGATTGTTCCTTGTTTCATCATTGCCACGTCCCATTTACATTCGCTTGTTGTGTGCGTAgggtgtgggggt                                                                                         >  1:7002190/1‑93 (MQ=255)
aCTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAgggagtgggggt                                                                                         <  1:670671/93‑1 (MQ=255)
aCTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAgggagtgggggt                                                                                         >  1:5035264/1‑93 (MQ=255)
 cTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGtt                                                                                        >  1:6106578/1‑93 (MQ=255)
 cTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGtt                                                                                        >  1:4822319/1‑93 (MQ=255)
  tGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTTGCTTGCTGCGTGTGCAGGGAGTGGGGGTTa                                                                                       >  1:7014931/1‑93 (MQ=255)
  tGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTa                                                                                       >  2:5072890/1‑93 (MQ=255)
  tGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTa                                                                                       >  2:4518584/1‑93 (MQ=255)
  tGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGAGTGCAGGGGGGGGGGGTTa                                                                                       >  1:5256192/1‑93 (MQ=255)
     cTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAttt                                                                                    >  1:3317049/1‑93 (MQ=255)
      ttCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtttt                                                                                   >  2:7181157/1‑93 (MQ=255)
       tCTGCTGACCTGCATGGATTGCTCCTTTTTTCGACATTGTCCCGTCCCATTTACATTCCCTTGCCGAGGGTGCAGGGAGGGGGGGCTAttttt                                                                                  >  1:2137740/1‑93 (MQ=255)
         tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGtttttttttt                                                                                >  2:228348/1‑91 (MQ=255)
         tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCt                                                                                >  1:6906585/1‑93 (MQ=255)
         tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCt                                                                                >  2:179088/1‑93 (MQ=255)
          gctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCtt                                                                               <  1:1296466/93‑1 (MQ=255)
           ctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCttt                                                                              <  1:3772788/93‑1 (MQ=255)
             gACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGc                                                                            <  2:5750539/93‑1 (MQ=255)
             gACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGc                                                                            <  1:2344626/93‑1 (MQ=255)
             gACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGc                                                                            <  2:6120037/93‑1 (MQ=255)
                cTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGa                                                                         >  1:5961300/1‑93 (MQ=255)
                cTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGa                                                                         >  2:2868180/1‑93 (MQ=255)
                cTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGa                                                                         >  1:2234272/1‑93 (MQ=255)
                 tGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGaa                                                                        >  2:118075/1‑93 (MQ=255)
                 tGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGaa                                                                        >  2:3743474/1‑93 (MQ=255)
                  gCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAg                                                                       <  1:7574147/93‑1 (MQ=255)
                    ctgcaTTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAggg                                                                     <  2:1048960/89‑1 (MQ=255)
                    aTGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAggg                                                                     >  1:2947994/1‑93 (MQ=255)
                     tGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgggg                                                                    >  2:600610/1‑93 (MQ=255)
                     tGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgggg                                                                    <  1:6526527/93‑1 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             >  1:368870/1‑93 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             <  1:3350296/93‑1 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             <  2:2236313/93‑1 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             <  2:3354852/93‑1 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             <  1:101718/93‑1 (MQ=255)
                             tCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAg                                                            >  1:7121732/1‑93 (MQ=255)
                                      cGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAt                                                   <  2:5297021/93‑1 (MQ=255)
                                       gACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAtt                                                  >  2:1668702/1‑93 (MQ=255)
                                       gACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAtt                                                  <  1:7386111/93‑1 (MQ=255)
                                         cATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTgg                                                >  2:4128183/1‑93 (MQ=255)
                                         cATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTgg                                                >  1:2450670/1‑93 (MQ=255)
                                             gTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCa                                            >  2:4613318/1‑93 (MQ=255)
                                              tCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCaa                                           >  2:77801/1‑93 (MQ=255)
                                                 cGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTTCGGCAGGGGCCGCAAAGGTCCAGAATTGGCTCAAttt                                        >  2:3857647/1‑93 (MQ=255)
                                                  gTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTc                                       >  2:6415753/1‑93 (MQ=255)
                                                     ccATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCt                                    >  1:2929375/1‑93 (MQ=255)
                                                       aTTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcc                                  >  2:3566825/1‑93 (MQ=255)
                                                       aTTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcc                                  <  2:1553329/93‑1 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 <  2:4014173/93‑1 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 >  1:904714/1‑93 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 <  1:1445475/93‑1 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 <  2:1260349/93‑1 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 <  1:2663206/93‑1 (MQ=255)
                                                         ttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccat                                >  2:3186891/1‑93 (MQ=255)
                                                         ttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccat                                <  2:4462669/93‑1 (MQ=255)
                                                         ttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccat                                <  2:1874225/93‑1 (MQ=255)
                                                          tACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatc                               <  1:432496/93‑1 (MQ=255)
                                                          tACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatc                               <  2:7563052/93‑1 (MQ=255)
                                                          tACATTCGCTTGCTGCGAGTGCAGGGAGCGGGGGTTATTTTTATTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatc                               >  2:5406119/1‑93 (MQ=255)
                                                             aTTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAg                            <  1:3430909/93‑1 (MQ=255)
                                                               tCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGa                          >  1:3526717/1‑93 (MQ=255)
                                                                 gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        <  1:2610203/93‑1 (MQ=255)
                                                                 gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        >  1:3900894/1‑93 (MQ=255)
                                                                 gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        <  2:5892473/93‑1 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      >  1:4864547/1‑93 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      <  1:5794231/93‑1 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCACCCAGGAAggg                      <  1:1647869/93‑1 (MQ=255)
                                                                    tgctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGt                     <  1:2045905/93‑1 (MQ=255)
                                                                    tgctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGt                     <  1:5596629/93‑1 (MQ=255)
                                                                     gctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGtt                    <  2:5634162/93‑1 (MQ=255)
                                                                     gctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGtt                    >  2:468510/1‑93 (MQ=255)
                                                                      ctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGttt                   <  2:6840533/93‑1 (MQ=255)
                                                                      ctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGttt                   <  2:4925193/93‑1 (MQ=255)
                                                                      ctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGttt                   <  2:6984744/93‑1 (MQ=255)
                                                                       tgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTg                  >  2:6654757/1‑93 (MQ=255)
                                                                       tgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTg                  >  1:7881408/1‑93 (MQ=255)
                                                                       tgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTg                  >  2:2541896/1‑93 (MQ=255)
                                                                        gcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGt                 <  1:4006667/93‑1 (MQ=255)
                                                                        gcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGt                 >  1:1758200/1‑93 (MQ=255)
                                                                         cGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTa                <  2:7646201/93‑1 (MQ=255)
                                                                         cGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTa                <  1:1194385/93‑1 (MQ=255)
                                                                          gtgtGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTaa               <  1:6038291/93‑1 (MQ=255)
                                                                          gtgtGCAGGGAGTGGGGGGTATTGTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAATTACCTCCATCCAGGAAGGGATTGtta               >  2:7898181/1‑91 (MQ=255)
                                                                          gtgtGCAGGGAGGGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTaa               >  2:2970532/1‑93 (MQ=255)
                                                                           tgtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              <  2:4326871/93‑1 (MQ=255)
                                                                            gtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACa             >  2:757361/1‑93 (MQ=255)
                                                                             tgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAg            <  2:4237313/93‑1 (MQ=255)
                                                                             tgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAg            <  1:7836414/93‑1 (MQ=255)
                                                                             tgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAg            <  1:4456335/93‑1 (MQ=255)
                                                                               cAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGaa          <  2:850034/93‑1 (MQ=255)
                                                                                aGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAAt         >  1:1543913/1‑93 (MQ=255)
                                                                                 gggagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATa        <  1:2506704/93‑1 (MQ=255)
                                                                                    agtgtgggtTATTTTTCTGTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCa     <  2:5236762/93‑1 (MQ=255)
                                                                                    agtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCa     <  1:5464827/93‑1 (MQ=255)
                                                                                     gtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAt    >  1:1078026/1‑93 (MQ=255)
                                                                                      tgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATa   >  2:712552/1‑93 (MQ=255)
                                                                                      tgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATa   >  2:2359071/1‑93 (MQ=255)
                                                                                      tgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATa   >  2:7379067/1‑93 (MQ=255)
                                                                                       gggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATaa  >  1:6332236/1‑93 (MQ=255)
                                                                                       gggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATaa  >  1:4728136/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA  >  NC_000913/4083381‑4083560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 210 total aligned reads displayed.
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  NC_000913/4083378‑4083564
                                                                                             |                                                                                             
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGG                                                                                               >  SRR4302543.4596845/1‑94 (MQ=60)
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGG                                                                                               <  SRR4302543.7052412/94‑1 (MQ=60)
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGG                                                                                               <  SRR4302543.7096772/94‑1 (MQ=60)
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGG                                                                                               >  SRR4302543.2051599/1‑94 (MQ=60)
 GAACTTCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGG                                                                                              <  SRR4302543.6236267/94‑1 (MQ=60)
  AACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGT                                                                                             <  SRR4302543.1684134/94‑1 (MQ=60)
  AACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGT                                                                                             >  SRR4302543.5081067/1‑94 (MQ=60)
  AACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGT                                                                                             >  SRR4302543.7483400/1‑94 (MQ=60)
   ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTT                                                                                            <  SRR4302543.676844/94‑1 (MQ=60)
   ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTT                                                                                            >  SRR4302543.4866334/1‑94 (MQ=60)
   ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTT                                                                                            >  SRR4302543.6164432/1‑94 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTTGCTTGCTGCGTGTGCAGGGAGTGGGGGTTA                                                                                           >  SRR4302543.7081916/1‑94 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTA                                                                                           >  SRR4302543.2961381/1‑94 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTA                                                                                           >  SRR4302543.3878031/1‑94 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTA                                                                                           >  SRR4302543.7081915/1‑94 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGAGTGCAGGGGGGGGGGGTTA                                                                                           >  SRR4302543.5303599/1‑94 (MQ=60)
     TGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAT                                                                                          >  SRR4302543.881041/1‑94 (MQ=60)
      TCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATT                                                                                         <  SRR4302543.3613476/94‑1 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTT                                                                                        >  SRR4302543.3346982/1‑94 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTT                                                                                        >  SRR4302543.7109301/1‑94 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTT                                                                                        >  SRR4302543.15449/1‑94 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTT                                                                                        >  SRR4302543.7138608/1‑94 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGGGGGGGTTATTT                                                                                        >  SRR4302543.2171123/1‑94 (MQ=60)
        CTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       >  SRR4302543.7430808/1‑94 (MQ=60)
         TTCTGCTGACCTGCATGGATTGCTCCTTTTTTCGACATTGTCCCGTCCCATTTACATTCCCTTGCCGAGGGTGCAGGGAGGGGGGGCTATTTTT                                                                                      >  SRR4302543.2156874/1‑94 (MQ=55)
         TTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACCTTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTT                                                                                      <  SRR4302543.127006/94‑1 (MQ=60)
         TTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTT                                                                                      >  SRR4302543.1145222/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302543.1132134/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302543.6971488/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302543.6952643/1‑94 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   >  SRR4302543.2270746/1‑94 (MQ=60)
             GCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTT                                                                                  <  SRR4302543.1307997/94‑1 (MQ=60)
              cttgCCTGCAGGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTCCATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 <  SRR4302543.1413875/90‑1 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 >  SRR4302543.6294879/1‑94 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 <  SRR4302543.3806010/94‑1 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 >  SRR4302543.2088100/1‑94 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                <  SRR4302543.888195/94‑1 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302543.2262864/94‑1 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302543.2365896/94‑1 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302543.7757807/94‑1 (MQ=60)
                  CCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGA                                                                             >  SRR4302543.2254483/1‑94 (MQ=60)
                  CCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGA                                                                             >  SRR4302543.6015883/1‑94 (MQ=60)
                  CCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGA                                                                             >  SRR4302543.6015884/1‑94 (MQ=60)
                  CCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGCTATTTTTCTTTGCGGA                                                                             >  SRR4302543.7039225/1‑94 (MQ=60)
                  CCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGGGTGCAGGGCGGGGGGGTTATTTTTCTTTGCGGA                                                                             >  SRR4302543.7039224/1‑94 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            <  SRR4302543.161931/94‑1 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            <  SRR4302543.6436041/94‑1 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           <  SRR4302543.2839461/94‑1 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           >  SRR4302543.5946203/1‑94 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           <  SRR4302543.1697672/94‑1 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           >  SRR4302543.2097123/1‑94 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302543.7646186/94‑1 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302543.37588/94‑1 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302543.3783367/94‑1 (MQ=60)
                      CATGGATTGCTGCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTACAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGG                                                                         <  SRR4302543.2997476/94‑1 (MQ=60)
                      CATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGG                                                                         >  SRR4302543.2974757/1‑94 (MQ=60)
                       ATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGG                                                                        <  SRR4302543.7592545/94‑1 (MQ=60)
                        TGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGC                                                                       <  SRR4302543.6587981/94‑1 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCC                                                                      >  SRR4302543.6688324/1‑94 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCC                                                                      >  SRR4302543.6688325/1‑94 (MQ=60)
                          GATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCG                                                                     >  SRR4302543.4875042/1‑94 (MQ=60)
                           ATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGC                                                                    <  SRR4302543.3778963/94‑1 (MQ=60)
                            TTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCA                                                                   >  SRR4302543.4810531/1‑94 (MQ=60)
                             TGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAA                                                                  >  SRR4302543.5658541/1‑94 (MQ=60)
                             TGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAA                                                                  <  SRR4302543.2971782/94‑1 (MQ=60)
                             TGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAA                                                                  >  SRR4302543.7069656/1‑94 (MQ=60)
                              GCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAA                                                                 >  SRR4302543.2640038/1‑94 (MQ=60)
                              GCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAA                                                                 >  SRR4302543.6404092/1‑94 (MQ=60)
                              GCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAA                                                                 >  SRR4302543.372272/1‑94 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                >  SRR4302543.6186037/1‑94 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                <  SRR4302543.102657/94‑1 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                <  SRR4302543.339459/94‑1 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                <  SRR4302543.3380460/94‑1 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                >  SRR4302543.7190919/1‑94 (MQ=60)
                                TCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGG                                                               >  SRR4302543.1532401/1‑94 (MQ=60)
                                TCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGG                                                               >  SRR4302543.5504779/1‑94 (MQ=60)
                                 CCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGT                                                              >  SRR4302543.4854578/1‑94 (MQ=60)
                                 CCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGT                                                              <  SRR4302543.516930/94‑1 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             >  SRR4302543.5174524/1‑94 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             >  SRR4302543.3857353/1‑94 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             >  SRR4302543.7919212/1‑94 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           >  SRR4302543.496259/1‑94 (MQ=60)
                                     GTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAG                                                          >  SRR4302543.7077351/1‑94 (MQ=60)
                                      TTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGA                                                         >  SRR4302543.1293983/1‑94 (MQ=60)
                                      TTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGA                                                         <  SRR4302543.2774898/94‑1 (MQ=60)
                                        TCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAT                                                       <  SRR4302543.1422715/94‑1 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      >  SRR4302543.6881717/1‑94 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      >  SRR4302543.604998/1‑94 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      <  SRR4302543.7147227/94‑1 (MQ=60)
                                          CACATTGTCCCGTCCCATTTACCTTCGCTTGCTGCCTGTGCAGGGAGTTGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTG                                                     <  SRR4302543.1410298/94‑1 (MQ=60)
                                           ACATTGTCACGTGTCATTGACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGG                                                    <  SRR4302543.4872914/94‑1 (MQ=60)
                                           ACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGG                                                    >  SRR4302543.2472777/1‑94 (MQ=60)
                                            CATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGC                                                   <  SRR4302543.1167404/94‑1 (MQ=60)
                                            CATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGC                                                   >  SRR4302543.3576183/1‑94 (MQ=60)
                                             ATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGGCGCAAAGGTCCAGAATTGGCT                                                  >  SRR4302543.2551118/1‑94 (MQ=60)
                                             ATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCT                                                  >  SRR4302543.1281714/1‑94 (MQ=60)
                                             ATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCT                                                  >  SRR4302543.5484336/1‑94 (MQ=60)
                                             ATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCT                                                  >  SRR4302543.3457134/1‑94 (MQ=60)
                                               TGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCA                                                <  SRR4302543.5740697/94‑1 (MQ=60)
                                               TGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCA                                                <  SRR4302543.7347284/94‑1 (MQ=60)
                                                 TCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAT                                              <  SRR4302543.4389398/94‑1 (MQ=60)
                                                  CACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCCAAGGTCCAGAATTGGCTCACTT                                             >  SRR4302543.520740/1‑94 (MQ=60)
                                                  CACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATT                                             >  SRR4302543.2437191/1‑94 (MQ=60)
                                                   ACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTT                                            >  SRR4302543.4539280/1‑94 (MQ=60)
                                                   ACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTT                                            >  SRR4302543.4539281/1‑94 (MQ=60)
                                                    CGTCCCATTTACATTCGCTTGCTTCGCGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGTCGCAAAGGGACAGAATTGGATCAATTTC                                           >  SRR4302543.5372185/1‑94 (MQ=60)
                                                    CGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTC                                           >  SRR4302543.159952/1‑94 (MQ=60)
                                                    CGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTC                                           >  SRR4302543.5730942/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302543.2000334/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302543.1648571/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302543.4967943/1‑94 (MQ=60)
                                                      TCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCC                                         >  SRR4302543.5894861/1‑94 (MQ=60)
                                                      TCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCC                                         <  SRR4302543.6980407/94‑1 (MQ=60)
                                                       CCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCT                                        >  SRR4302543.7951116/1‑94 (MQ=60)
                                                       CCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCT                                        >  SRR4302543.2955983/1‑94 (MQ=60)
                                                        CCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTC                                       >  SRR4302543.1033022/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      <  SRR4302543.2475676/94‑1 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      >  SRR4302543.5541859/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      <  SRR4302543.3354007/94‑1 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGGGTGCAGGGAGTCTGGGTTATGTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      <  SRR4302543.3354006/94‑1 (MQ=60)
                                                          ATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCA                                     >  SRR4302543.1717033/1‑94 (MQ=60)
                                                          ATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCA                                     >  SRR4302543.3551138/1‑94 (MQ=60)
                                                          ATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCA                                     >  SRR4302543.912681/1‑94 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    <  SRR4302543.2687351/94‑1 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    <  SRR4302543.637427/94‑1 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    <  SRR4302543.4011388/94‑1 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    <  SRR4302543.1458532/94‑1 (MQ=60)
                                                             TACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCC                                  <  SRR4302543.436548/94‑1 (MQ=60)
                                                              ACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCA                                 >  SRR4302543.559947/1‑94 (MQ=60)
                                                              ACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCA                                 >  SRR4302543.2679592/1‑94 (MQ=60)
                                                               CATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAG                                >  SRR4302543.3218706/1‑94 (MQ=60)
                                                                ATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGG                               >  SRR4302543.2327983/1‑94 (MQ=60)
                                                                ATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGG                               >  SRR4302543.7723954/1‑94 (MQ=60)
                                                                 TTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGA                              <  SRR4302543.3297447/94‑1 (MQ=60)
                                                                 TTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGA                              >  SRR4302543.3558151/1‑94 (MQ=60)
                                                                 TTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCACTCCATCCAGGA                              >  SRR4302543.3558150/1‑94 (MQ=60)
                                                                  TCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAA                             <  SRR4302543.2178885/94‑1 (MQ=60)
                                                                   CGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAG                            >  SRR4302543.3935339/1‑94 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           <  SRR4302543.7742512/94‑1 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           <  SRR4302543.2633898/94‑1 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           <  SRR4302543.2633899/94‑1 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          >  SRR4302543.4908892/1‑94 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302543.2423552/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302543.840391/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302543.4392378/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302543.4392379/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302543.5846947/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCACCCAGGAAGGGT                         <  SRR4302543.1662606/94‑1 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        <  SRR4302543.2064244/94‑1 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        <  SRR4302543.5647615/94‑1 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        >  SRR4302543.6489735/1‑94 (MQ=60)
                                                                        GCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTT                       <  SRR4302543.105407/94‑1 (MQ=60)
                                                                        GCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTT                       <  SRR4302543.2444126/94‑1 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      <  SRR4302543.4536834/94‑1 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302543.7955577/1‑94 (MQ=60)
                                                                          TGCGTGTGCAGTGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGT                     <  SRR4302543.6104255/94‑1 (MQ=60)
                                                                          TGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGT                     >  SRR4302543.1773906/1‑94 (MQ=60)
                                                                          TGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGT                     >  SRR4302543.1773907/1‑94 (MQ=60)
                                                                           GCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTA                    <  SRR4302543.4042428/94‑1 (MQ=60)
                                                                           GCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTA                    <  SRR4302543.2653222/94‑1 (MQ=60)
                                                                            CGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAA                   >  SRR4302543.3374887/1‑94 (MQ=60)
                                                                            CGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAA                   >  SRR4302543.6151953/1‑94 (MQ=60)
                                                                            CGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAA                   <  SRR4302543.1205154/94‑1 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  <  SRR4302543.6095416/94‑1 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302543.2515010/1‑94 (MQ=60)
                                                                               GTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAG                <  SRR4302543.2015839/94‑1 (MQ=60)
                                                                                TGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGA               <  SRR4302543.4496708/94‑1 (MQ=60)
                                                                                TGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGA               <  SRR4302543.7910176/94‑1 (MQ=60)
                                                                                TGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGA               <  SRR4302543.2595645/94‑1 (MQ=60)
                                                                                  CAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAAT             >  SRR4302543.3997683/1‑94 (MQ=60)
                                                                                   AGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATA            <  SRR4302543.1778199/94‑1 (MQ=60)
                                                                                    GGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAC           <  SRR4302543.7560131/94‑1 (MQ=60)
                                                                                    GGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAC           <  SRR4302543.2529467/94‑1 (MQ=60)
                                                                                      GAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCA         <  SRR4302543.359035/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        <  SRR4302543.5514347/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        <  SRR4302543.6066648/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        >  SRR4302543.1087717/1‑94 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        >  SRR4302543.3426531/1‑94 (MQ=60)
                                                                                        GTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATA       >  SRR4302543.4172423/1‑94 (MQ=60)
                                                                                        GTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATA       <  SRR4302543.2790912/94‑1 (MQ=60)
                                                                                         TGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAGCAGAATACCATAA      <  SRR4302543.7950865/94‑1 (MQ=60)
                                                                                         TGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA      >  SRR4302543.6391789/1‑94 (MQ=60)
                                                                                         TGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA      <  SRR4302543.3945763/94‑1 (MQ=60)
                                                                                         TGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA      <  SRR4302543.4836098/94‑1 (MQ=60)
                                                                                         TGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA      >  SRR4302543.4771331/1‑94 (MQ=60)
                                                                                          GGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAAT     <  SRR4302543.3882531/94‑1 (MQ=60)
                                                                                          GGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAAT     >  SRR4302543.1940584/1‑94 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    >  SRR4302543.7325485/1‑94 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    >  SRR4302543.1191615/1‑94 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    <  SRR4302543.6250621/94‑1 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   >  SRR4302543.5393946/1‑94 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   >  SRR4302543.2879496/1‑94 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   >  SRR4302543.8007532/1‑94 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGGCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATcatat   >  SRR4302543.5393945/1‑89 (MQ=60)
                                                                                             GGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  <  SRR4302543.3493887/94‑1 (MQ=60)
                                                                                             GGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  SRR4302543.1329751/1‑94 (MQ=60)
                                                                                             GGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  SRR4302543.920846/1‑94 (MQ=60)
                                                                                             GGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  <  SRR4302543.7928424/94‑1 (MQ=60)
                                                                                             GGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  SRR4302543.7460135/1‑94 (MQ=60)
                                                                                             GGTTATTTTACTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  SRR4302543.3142984/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  NC_000913/4083378‑4083564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑