Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,083,858	T→C	V88A (GTT→GCT)	fdhD →	affects formate dehydrogenase‑N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,083,858	0	T	C	99.7%	1120.2 / ‑5.3	360	V88A (GTT→GCT)	fdhD	affects formate dehydrogenase‑N
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (174/184); total (175/185)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

Only 100 of 403 total aligned reads displayed.

GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGAC  >  NC_000913/4083769‑4083947

  ccAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctcctt                                                                                      >  1:6106763/1‑93 (MQ=255)

   cAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctccttc                                                                                     >  2:2857254/1‑93 (MQ=255)

    aaaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctccttcc                                                                                    >  2:7601482/1‑93 (MQ=255)

    aaaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctccttcc                                                                                    >  2:4313510/1‑93 (MQ=255)

    aaaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctccttcc                                                                                    >  2:3156391/1‑93 (MQ=255)

    aaaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTAGctccttcc                                                                                    <  1:4456316/93‑1 (MQ=255)

     aaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCtccttcct                                                                                   >  1:1111194/1‑93 (MQ=255)

      aGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTg                                                                                  <  1:149116/93‑1 (MQ=255)

       gACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGt                                                                                 <  2:85331/93‑1 (MQ=255)

        aCCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTa                                                                                >  2:2154601/1‑93 (MQ=255)

         ccTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTaa                                                                               <  1:815893/93‑1 (MQ=255)

          cTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAAt                                                                              <  2:4271572/93‑1 (MQ=255)

          cTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAAt                                                                              <  1:3761221/93‑1 (MQ=255)

           ttGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATg                                                                             <  1:1254781/93‑1 (MQ=255)

           ttGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATg                                                                             <  1:3357882/93‑1 (MQ=255)

           ttGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGAATCGATGTCGTTCCTTCCTGAAATg                                                                             >  2:2374243/1‑93 (MQ=255)

            tGAGTACTTTGCGCTCGGTTTTTCGCTTTCGGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATgg                                                                            <  1:7128649/93‑1 (MQ=255)

             taGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGt                                                                           <  2:3236288/92‑1 (MQ=255)

             gAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGt                                                                           <  1:2330510/93‑1 (MQ=255)

             gAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGt                                                                           <  2:4180339/93‑1 (MQ=255)

              aGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGtt                                                                          >  2:6314266/1‑92 (MQ=255)

              aGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTc                                                                          >  1:1690035/1‑93 (MQ=255)

                tACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCtt                                                                        >  1:3177516/1‑93 (MQ=255)

                 aCTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTg                                                                       <  2:6161552/93‑1 (MQ=255)

                 aCTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTg                                                                       <  2:7131767/93‑1 (MQ=255)

                  cTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGa                                                                      >  1:3969891/1‑93 (MQ=255)

                  cTTTGCGCTCGGATTTTCGCTTTCCGGGGGGATTATCGCGAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGACTTGa                                                                      <  2:7041294/93‑1 (MQ=255)

                   tttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGaa                                                                     >  2:5782322/1‑93 (MQ=255)

                   tttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGaa                                                                     >  1:6480449/1‑93 (MQ=255)

                   tttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGaa                                                                     <  2:3645844/93‑1 (MQ=255)

                   tttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGaa                                                                     <  1:6246156/93‑1 (MQ=255)

                    ttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAg                                                                    <  2:1302841/93‑1 (MQ=255)

                      gcgcTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTa                                                                  <  1:6974687/93‑1 (MQ=255)

                      gcgcTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTa                                                                  >  2:6814015/1‑93 (MQ=255)

                          tCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACaaa                                                              <  2:5882032/93‑1 (MQ=255)

                          ccGGTTTTTCGCTTTCCGAGGGGATTATCGGAAGTCCGCGCGATATCTGCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACaaa                                                              <  1:5597288/92‑1 (MQ=255)

                           ccgTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAt                                                             <  1:3224246/91‑1 (MQ=255)

                           cGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTGCTTCCTGTAATGGTCTTGAAGTACAAAt                                                             <  1:7542305/93‑1 (MQ=255)

                           cGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAt                                                             <  2:4609898/93‑1 (MQ=255)

                            ggTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAtt                                                            <  2:1198409/93‑1 (MQ=255)

                            ggTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAtt                                                            >  2:2009876/1‑93 (MQ=255)

                             gTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTg                                                           >  1:6708615/1‑93 (MQ=255)

                               ttttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAg                                                         <  1:981003/93‑1 (MQ=255)

                                tttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGc                                                        <  2:7401305/93‑1 (MQ=255)

                                tttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGc                                                        <  1:2833619/93‑1 (MQ=255)

                                 ttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCt                                                       <  1:1357689/93‑1 (MQ=255)

                                 ttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCt                                                       <  2:2534905/93‑1 (MQ=255)

                                 ttCGCGTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCt                                                       <  1:1007811/93‑1 (MQ=255)

                                  tCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCtt                                                      >  2:47479/1‑93 (MQ=255)

                                    gCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTc                                                    <  1:7322357/93‑1 (MQ=255)

                                    gCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTc                                                    <  2:7664914/93‑1 (MQ=255)

                                    gCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTc                                                    >  2:2289428/1‑93 (MQ=255)

                                    gCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTc                                                    <  1:785083/93‑1 (MQ=255)

                                      tttCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCa                                                  <  2:4691104/93‑1 (MQ=255)

                                      tttCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCa                                                  >  2:5525669/1‑93 (MQ=255)

                                        tCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgc                                                <  1:4612210/93‑1 (MQ=255)

                                         ccGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgcc                                               <  1:3927939/93‑1 (MQ=255)

                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             >  2:4933563/1‑93 (MQ=255)

                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             >  1:5811447/1‑93 (MQ=255)

                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             <  2:2758040/93‑1 (MQ=255)

                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             >  1:3410994/1‑93 (MQ=255)

                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             <  1:1451168/93‑1 (MQ=255)

                                            agtggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgcc                                            <  2:5323655/90‑1 (MQ=255)

                                            aGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgcc                                            >  2:1861360/1‑93 (MQ=255)

                                            aGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgcc                                            >  1:4267950/1‑93 (MQ=255)

                                            aGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgcc                                            >  2:7353551/1‑93 (MQ=255)

                                            aGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTCAGCTTTCCAgccgcc                                            >  2:4450612/1‑93 (MQ=255)

                                             ggggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGccgccg                                           >  2:388653/1‑93 (MQ=255)

                                               ggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCt                                         >  2:4018676/1‑93 (MQ=255)

                                               ggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCt                                         >  1:5040755/1‑93 (MQ=255)

                                                gATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCtt                                        >  1:5410175/1‑93 (MQ=255)

                                                  ttATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTa                                      <  2:667383/93‑1 (MQ=255)

                                                    aTCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATg                                    <  1:1921879/93‑1 (MQ=255)

                                                     tCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATgg                                   <  1:3679255/93‑1 (MQ=255)

                                                     tCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATgg                                   >  2:1573448/1‑93 (MQ=255)

                                                     tCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATgg                                   <  2:1697967/93‑1 (MQ=255)

                                                       gAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATgggg                                 <  1:1847009/93‑1 (MQ=255)

                                                        aaaGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGt                                <  1:4389200/93‑1 (MQ=255)

                                                           gTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGa                             <  1:6824539/93‑1 (MQ=255)

                                                             ccGCTCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAg                           <  2:5041449/93‑1 (MQ=255)

                                                               gcgcgATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGa                         >  2:4542911/1‑93 (MQ=255)

                                                               gcgcgATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGa                         <  1:591163/93‑1 (MQ=255)

                                                               gcgcgATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGa                         <  2:3014739/93‑1 (MQ=255)

                                                                 gcgATATCTTCGGCATGGAGTTCGCTCCTTCCTGTAAGTGTCGTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAgc                       <  1:6385762/93‑1 (MQ=255)

                                                                  cgATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAgcg                      >  2:4109892/1‑93 (MQ=255)

                                                                    atatCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGcgcc                    >  1:6007533/1‑93 (MQ=255)

                                                                      atCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGcgccgc                  <  2:6469017/93‑1 (MQ=255)

                                                                      atCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGcgccgc                  <  1:3033268/93‑1 (MQ=255)

                                                                       tCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCgccgcc                 >  1:4980405/1‑93 (MQ=255)

                                                                        cTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGccgccg                >  2:5598373/1‑93 (MQ=255)

                                                                         ttCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCgg               <  1:2557164/93‑1 (MQ=255)

                                                                           cGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGgc             <  2:3493231/93‑1 (MQ=255)

                                                                            ggCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGgcc            >  1:2207748/1‑92 (MQ=255)

                                                                              cATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCt          >  2:6056315/1‑93 (MQ=255)

                                                                                tGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTgg        >  2:656937/1‑93 (MQ=255)

                                                                                tGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTgg        >  1:4829383/1‑93 (MQ=255)

                                                                                tGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTgg        >  2:1817937/1‑93 (MQ=255)

                                                                                  gATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCg      >  1:7384420/1‑93 (MQ=255)

                                                                                    tGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCggg    <  2:7765798/93‑1 (MQ=255)

                                                                                     gTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGa   >  2:6976846/1‑93 (MQ=255)

GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGAC  >  NC_000913/4083769‑4083947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 207 total aligned reads displayed.

GTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTAC  >  NC_000913/4083766‑4083951

GTCGCCCAAAGACCTTGAGTACTTTGCGCTTGGTGTTTCGCCTTCCGAGGGGATTATCGAAAATCCGCGCGATATCTTCGGCATGGATGTCGCT                                                                                              <  SRR4302543.5409750/94‑1 (MQ=60)

GTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCT                                                                                              >  SRR4302543.2178526/1‑94 (MQ=60)

GTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCT                                                                                              >  SRR4302543.2652731/1‑94 (MQ=60)

  CGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCC                                                                                            >  SRR4302543.1656472/1‑94 (MQ=60)

   GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCT                                                                                           >  SRR4302543.4062509/1‑94 (MQ=60)

   GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCT                                                                                           <  SRR4302543.4831185/94‑1 (MQ=60)

   GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCT                                                                                           >  SRR4302543.1833329/1‑94 (MQ=60)

   GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCT                                                                                           <  SRR4302543.3279243/94‑1 (MQ=60)

   GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCT                                                                                           <  SRR4302543.6805811/94‑1 (MQ=60)

   GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCT                                                                                           <  SRR4302543.4360054/94‑1 (MQ=60)

   CCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCT                                                                                           <  SRR4302543.1236438/94‑1 (MQ=60)

    CCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTT                                                                                          <  SRR4302543.1611168/94‑1 (MQ=60)

    CCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTT                                                                                          >  SRR4302543.6164620/1‑94 (MQ=60)

     CCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTC                                                                                         >  SRR4302543.5545434/1‑94 (MQ=60)

     CCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTC                                                                                         >  SRR4302543.1891663/1‑94 (MQ=60)

     CCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTC                                                                                         >  SRR4302543.2329800/1‑94 (MQ=60)

      CAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCC                                                                                        >  SRR4302543.4621109/1‑94 (MQ=60)

       AAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCT                                                                                       <  SRR4302543.1465530/94‑1 (MQ=60)

       AAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCT                                                                                       <  SRR4302543.5420470/94‑1 (MQ=60)

       AAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCT                                                                                       >  SRR4302543.1121225/1‑94 (MQ=60)

       AAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCT                                                                                       >  SRR4302543.1121224/1‑94 (MQ=60)

       AAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTAGCTCCTTCCT                                                                                       <  SRR4302543.4496688/94‑1 (MQ=60)

        AAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTG                                                                                      >  SRR4302543.132806/1‑94 (MQ=60)

        AAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTG                                                                                      >  SRR4302543.7423996/1‑94 (MQ=60)

         AGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGT                                                                                     <  SRR4302543.150407/94‑1 (MQ=60)

           ACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGAGATAGCTTCGGCATGGATGGCGCTGCTTGCTGTAA                                                                                   >  SRR4302543.651548/1‑94 (MQ=60)

           ACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCAGCGCGATATCTTCAGCATGGATGTCGCTCCTTCCTGTAA                                                                                   >  SRR4302543.651549/1‑94 (MQ=60)

            CCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAAT                                                                                  <  SRR4302543.823074/94‑1 (MQ=60)

            CCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAAT                                                                                  >  SRR4302543.3166297/1‑94 (MQ=60)

             CTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATG                                                                                 >  SRR4302543.5459559/1‑94 (MQ=60)

             CTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATG                                                                                 <  SRR4302543.3794325/94‑1 (MQ=60)

             CTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATG                                                                                 >  SRR4302543.3977665/1‑94 (MQ=60)

             CTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATG                                                                                 >  SRR4302543.5913333/1‑94 (MQ=60)

              TTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGG                                                                                >  SRR4302543.5647598/1‑94 (MQ=60)

              TTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGG                                                                                <  SRR4302543.3388091/94‑1 (MQ=60)

              TTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGG                                                                                <  SRR4302543.1266027/94‑1 (MQ=60)

              TTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGG                                                                                <  SRR4302543.2339847/94‑1 (MQ=60)

              CTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGG                                                                                <  SRR4302543.6691771/94‑1 (MQ=60)

               TGAGTACTTTGCGCTCGGTTTTTCGCTTTCGGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGT                                                                               <  SRR4302543.7197921/94‑1 (MQ=60)

               TGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGT                                                                               <  SRR4302543.5804025/94‑1 (MQ=60)

               TGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGT                                                                               >  SRR4302543.3482681/1‑94 (MQ=60)

                GAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTC                                                                              <  SRR4302543.2351666/94‑1 (MQ=60)

                GAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTC                                                                              >  SRR4302543.1705207/1‑94 (MQ=60)

                GAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTC                                                                              >  SRR4302543.3858453/1‑94 (MQ=60)

                 AGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCT                                                                             >  SRR4302543.3450960/1‑94 (MQ=60)

                  GTATTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTT                                                                            <  SRR4302543.496476/94‑1 (MQ=60)

                  GTACTTTGCGCTCGGTTTTTCGCTTTCCGGGGGGATTTTCGATTGTCCGCGCGCTCTCTTCGGCATGGATGTCGCTCCTTCCTGTGCTGGTCTT                                                                            <  SRR4302543.7115767/94‑1 (MQ=55)

                  GTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTT                                                                            <  SRR4302543.149611/94‑1 (MQ=60)

                  GTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTT                                                                            >  SRR4302543.3206151/1‑94 (MQ=60)

                  GTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTT                                                                            >  SRR4302543.1207956/1‑94 (MQ=60)

                   TACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGTTTATAGATAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCATTCCTGTAATGGTCTTG                                                                           >  SRR4302543.4131521/1‑94 (MQ=60)

                   TACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTG                                                                           <  SRR4302543.6734450/94‑1 (MQ=60)

                    ACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGA                                                                          >  SRR4302543.6247366/1‑94 (MQ=60)

                    ACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGA                                                                          >  SRR4302543.4005081/1‑94 (MQ=60)

                     CTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAA                                                                         >  SRR4302543.804670/1‑94 (MQ=60)

                     CTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAA                                                                         >  SRR4302543.6541508/1‑94 (MQ=60)

                      TTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAG                                                                        <  SRR4302543.6305082/94‑1 (MQ=60)

                       TTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGT                                                                       >  SRR4302543.1429508/1‑94 (MQ=60)

                       TTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGT                                                                       <  SRR4302543.5067829/94‑1 (MQ=60)

                        TGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTA                                                                      <  SRR4302543.2993722/94‑1 (MQ=60)

                         GCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTAC                                                                     <  SRR4302543.7435730/94‑1 (MQ=60)

                         GCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTAC                                                                     <  SRR4302543.6121852/94‑1 (MQ=60)

                         GCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTAC                                                                     <  SRR4302543.7040324/94‑1 (MQ=60)

                          CGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACA                                                                    <  SRR4302543.1809944/94‑1 (MQ=60)

                          CGCTCGGTTTTTCGCTTTCCGAGGGGATTATCCAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACA                                                                    <  SRR4302543.3883583/94‑1 (MQ=60)

                            CTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTAAAAA                                                                  >  SRR4302543.7005977/1‑94 (MQ=60)

                            CTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGGCTTGAAGTACAAA                                                                  >  SRR4302543.6052083/1‑94 (MQ=60)

                             TCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAT                                                                 <  SRR4302543.6227426/94‑1 (MQ=60)

                             TCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAT                                                                 <  SRR4302543.5648284/94‑1 (MQ=60)

                             CCGGTTTTTCGCTTTCCGAGGGGATTATCGGAAGTCCGCGCGATATCTGCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAT                                                                 <  SRR4302543.5648283/94‑1 (MQ=60)

                              CGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTGCTTCCTGTAATGGTCTTGAAGTACAAATT                                                                <  SRR4302543.7614181/94‑1 (MQ=60)

                              CGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATT                                                                >  SRR4302543.5049543/1‑94 (MQ=60)

                              CCGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATT                                                                <  SRR4302543.3253390/94‑1 (MQ=60)

                               GGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTG                                                               <  SRR4302543.6770616/94‑1 (MQ=60)

                               GGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTG                                                               >  SRR4302543.382806/1‑94 (MQ=60)

                               GGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTG                                                               <  SRR4302543.5875792/94‑1 (MQ=60)

                               GGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTG                                                               >  SRR4302543.6771963/1‑94 (MQ=60)

                                GTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGA                                                              >  SRR4302543.291348/1‑94 (MQ=60)

                                GTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGA                                                              >  SRR4302543.1539218/1‑94 (MQ=60)

                                 TTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAG                                                             <  SRR4302543.3439566/94‑1 (MQ=60)

                                  TTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACCAATTGAGC                                                            >  SRR4302543.6504269/1‑94 (MQ=60)

                                  TTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGC                                                            >  SRR4302543.1903433/1‑94 (MQ=60)

                                   TTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCT                                                           <  SRR4302543.438996/94‑1 (MQ=60)

                                   TTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCT                                                           <  SRR4302543.6331368/94‑1 (MQ=60)

                                   TTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCT                                                           <  SRR4302543.2859537/94‑1 (MQ=60)

                                    TTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTT                                                          >  SRR4302543.4785173/1‑94 (MQ=60)

                                    TTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTT                                                          <  SRR4302543.7496948/94‑1 (MQ=60)

                                    TTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTT                                                          <  SRR4302543.1369838/94‑1 (MQ=60)

                                    TTCGCGTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTT                                                          <  SRR4302543.1016727/94‑1 (MQ=60)

                                      CGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTC                                                        <  SRR4302543.7017801/94‑1 (MQ=60)

                                       GCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCC                                                       <  SRR4302543.7392875/94‑1 (MQ=60)

                                       GCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCC                                                       <  SRR4302543.791991/94‑1 (MQ=60)

                                        CTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCA                                                      <  SRR4302543.4989310/94‑1 (MQ=60)

                                        CTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCA                                                      >  SRR4302543.447038/1‑94 (MQ=60)

                                        CTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCA                                                      <  SRR4302543.6791275/94‑1 (MQ=60)

                                         TTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAG                                                     <  SRR4302543.108032/94‑1 (MQ=60)

                                          TTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGC                                                    <  SRR4302543.3741832/94‑1 (MQ=60)

                                          TTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGATTTCCAGC                                                    >  SRR4302543.2520886/1‑94 (MQ=60)

                                          TACCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGC                                                    <  SRR4302543.4430571/94‑1 (MQ=60)

                                           TCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCC                                                   >  SRR4302543.3395105/1‑94 (MQ=60)

                                           TCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCC                                                   <  SRR4302543.4654105/94‑1 (MQ=60)

                                            CCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCG                                                  <  SRR4302543.767075/94‑1 (MQ=60)

                                            CCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCG                                                  >  SRR4302543.491119/1‑94 (MQ=60)

                                            CCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCG                                                  <  SRR4302543.7878187/94‑1 (MQ=60)

                                            CCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCG                                                  <  SRR4302543.3962608/94‑1 (MQ=60)

                                             CGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGC                                                 <  SRR4302543.3500786/94‑1 (MQ=60)

                                             CGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGC                                                 >  SRR4302543.3441531/1‑94 (MQ=60)

                                             CGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGC                                                 >  SRR4302543.5864299/1‑94 (MQ=60)

                                             CGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGC                                                 >  SRR4302543.4184967/1‑94 (MQ=60)

                                             CGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGC                                                 <  SRR4302543.323998/94‑1 (MQ=60)

                                              GAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCC                                                <  SRR4302543.1464273/94‑1 (MQ=60)

                                              GAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCC                                                >  SRR4302543.7332687/1‑94 (MQ=60)

                                              GAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCC                                                >  SRR4302543.5977586/1‑94 (MQ=60)

                                              GAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCC                                                >  SRR4302543.4306724/1‑94 (MQ=60)

                                               AGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCG                                               >  SRR4302543.6186915/1‑94 (MQ=60)

                                               AGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCG                                               >  SRR4302543.4129094/1‑94 (MQ=60)

                                               AGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCC                                               >  SRR4302543.2809637/1‑94 (MQ=60)

                                                 GGGATTATCGAAAGTCCGCGCGCTATCTTCGGCATGGATGTCGCTCCTTCCTGCAATGGGCTTGAAGTACAAATTGAGCTTTCCAactcccgct                                             >  SRR4302543.5086604/1‑85 (MQ=60)

                                                 GGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCT                                             <  SRR4302543.236876/94‑1 (MQ=60)

                                                 GGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCT                                             >  SRR4302543.5086605/1‑94 (MQ=60)

                                                  GGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTT                                            >  SRR4302543.5780340/1‑94 (MQ=60)

                                                  GGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTT                                            >  SRR4302543.5459373/1‑94 (MQ=60)

                                                   GATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTT                                           <  SRR4302543.7433760/94‑1 (MQ=60)

                                                    ATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTA                                          >  SRR4302543.845089/1‑94 (MQ=60)

                                                    ATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTA                                          >  SRR4302543.7270629/1‑94 (MQ=60)

                                                    ATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTA                                          <  SRR4302543.1084665/94‑1 (MQ=60)

                                                     TTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTAT                                         >  SRR4302543.6094701/1‑94 (MQ=60)

                                                     TTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTAT                                         <  SRR4302543.1445592/94‑1 (MQ=60)

                                                      TATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATG                                        >  SRR4302543.4707932/1‑94 (MQ=60)

                                                      TATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATG                                        <  SRR4302543.177285/94‑1 (MQ=60)

                                                       ATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGG                                       >  SRR4302543.3104132/1‑94 (MQ=60)

                                                       ATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGG                                       <  SRR4302543.1939202/94‑1 (MQ=60)

                                                       ATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGG                                       <  SRR4302543.600038/94‑1 (MQ=60)

                                                        TCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGG                                      <  SRR4302543.3711575/94‑1 (MQ=60)

                                                        TCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGG                                      <  SRR4302543.6895658/94‑1 (MQ=60)

                                                         CGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGG                                     >  SRR4302543.2132967/1‑94 (MQ=60)

                                                         CGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGG                                     >  SRR4302543.209698/1‑94 (MQ=60)

                                                         CGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGG                                     <  SRR4302543.3362203/94‑1 (MQ=60)

                                                          GAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGT                                    <  SRR4302543.1863623/94‑1 (MQ=60)

                                                           AAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTT                                   <  SRR4302543.4428991/94‑1 (MQ=60)

                                                           AAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCCTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTT                                   >  SRR4302543.4472703/1‑94 (MQ=60)

                                                              GTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAA                                <  SRR4302543.6888959/94‑1 (MQ=60)

                                                              GTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAA                                >  SRR4302543.920489/1‑94 (MQ=60)

                                                              GTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAA                                <  SRR4302543.812314/94‑1 (MQ=60)

                                                               TCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAG                               <  SRR4302543.922689/94‑1 (MQ=60)

                                                               TCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAG                               <  SRR4302543.7972740/94‑1 (MQ=60)

                                                                CCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGG                              <  SRR4302543.2721723/94‑1 (MQ=60)

                                                                CCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGG                              >  SRR4302543.3223893/1‑94 (MQ=60)

                                                                 cgtatGATATCTTCGGCATGGTTGTCTTTCCTGCCTGTAATGGTCTTGAAGTACAAATTGAGCTTACCAGCCGCCGCTTTATGGGGTTGAAGGA                             <  SRR4302543.6185918/89‑1 (MQ=60)

                                                                 CGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGA                             >  SRR4302543.4533886/1‑94 (MQ=60)

                                                                 CGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGA                             <  SRR4302543.1358419/94‑1 (MQ=60)

                                                                 CGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGA                             >  SRR4302543.2895912/1‑94 (MQ=60)

                                                                  GCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAG                            <  SRR4302543.596592/94‑1 (MQ=60)

                                                                   CGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGC                           <  SRR4302543.1327165/94‑1 (MQ=60)

                                                                    GCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCG                          <  SRR4302543.6445865/94‑1 (MQ=60)

                                                                    GCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCG                          <  SRR4302543.339000/94‑1 (MQ=60)

                                                                    GCGATATCTTCGGCATGGAGTTCGCTCCTTCCTGTAAGTGTCGTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCG                          <  SRR4302543.6445866/94‑1 (MQ=60)

                                                                     CGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGC                         <  SRR4302543.3963452/94‑1 (MQ=60)

                                                                      GATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCC                        >  SRR4302543.5370412/1‑94 (MQ=60)

                                                                      GATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCC                        <  SRR4302543.786877/94‑1 (MQ=60)

                                                                      GATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCC                        >  SRR4302543.6062857/1‑94 (MQ=60)

                                                                       ATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCG                       >  SRR4302543.6108118/1‑94 (MQ=60)

                                                                         ATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCC                     >  SRR4302543.5025741/1‑94 (MQ=60)

                                                                         ATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCC                     <  SRR4302543.3060653/94‑1 (MQ=60)

                                                                         ATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCC                     <  SRR4302543.7049802/94‑1 (MQ=60)

                                                                           CTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGG                   >  SRR4302543.1484540/1‑94 (MQ=60)

                                                                           CTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGG                   >  SRR4302543.202140/1‑94 (MQ=60)

                                                                           CTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGG                   >  SRR4302543.2413672/1‑94 (MQ=60)

                                                                            TTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGG                  >  SRR4302543.3215566/1‑94 (MQ=60)

                                                                            TTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGG                  <  SRR4302543.2580540/94‑1 (MQ=60)

                                                                            TTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGG                  >  SRR4302543.7486552/1‑94 (MQ=60)

                                                                              CGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCC                >  SRR4302543.2227637/1‑94 (MQ=60)

                                                                                GCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCT              <  SRR4302543.1382928/94‑1 (MQ=60)

                                                                                 CATGGATGTGGCTCCTCCCTGTAATGGCCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTG             <  SRR4302543.3754818/94‑1 (MQ=60)

                                                                                 CATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTG             <  SRR4302543.2278684/94‑1 (MQ=60)

                                                                                 CATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTG             <  SRR4302543.3754819/94‑1 (MQ=60)

                                                                                 CATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTG             >  SRR4302543.6376160/1‑94 (MQ=60)

                                                                                  ATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGG            >  SRR4302543.589062/1‑94 (MQ=60)

                                                                                  ATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGG            >  SRR4302543.5222714/1‑94 (MQ=60)

                                                                                  ATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGG            >  SRR4302543.7525549/1‑94 (MQ=60)

                                                                                  ATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGG            >  SRR4302543.4873446/1‑94 (MQ=60)

                                                                                  ATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTGCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGG            <  SRR4302543.1059606/94‑1 (MQ=60)

                                                                                   TGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGC           >  SRR4302543.7066198/1‑94 (MQ=60)

                                                                                    GGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGATCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCG          <  SRR4302543.4364931/94‑1 (MQ=60)

                                                                                    GGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCG          >  SRR4302543.7455381/1‑94 (MQ=60)

                                                                                    GGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCG          >  SRR4302543.3263928/1‑94 (MQ=60)

                                                                                    GGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCG          >  SRR4302543.4555915/1‑94 (MQ=60)

                                                                                     GATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGG         <  SRR4302543.7312834/94‑1 (MQ=60)

                                                                                     GATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGG         >  SRR4302543.2712397/1‑94 (MQ=60)

                                                                                      ATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGG        >  SRR4302543.6790228/1‑94 (MQ=60)

                                                                                          CGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGT    >  SRR4302543.6642116/1‑94 (MQ=60)

                                                                                          CGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGT    >  SRR4302543.6240625/1‑94 (MQ=60)

                                                                                           GCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTA   >  SRR4302543.5951548/1‑94 (MQ=60)

                                                                                           GCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTA   >  SRR4302543.41900/1‑94 (MQ=60)

                                                                                           GCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTA   <  SRR4302543.3141889/94‑1 (MQ=60)

                                                                                            CTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTAC  >  SRR4302543.7776570/1‑94 (MQ=60)

                                                                                            CTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTAC  <  SRR4302543.447758/94‑1 (MQ=60)

                                                                                            CTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTAC  >  SRR4302543.7037025/1‑94 (MQ=60)

                                                                                            CTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTAC  <  SRR4302543.3654190/94‑1 (MQ=60)

                                                                                            CTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTAC  >  SRR4302543.2699552/1‑94 (MQ=60)

GTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTAC  >  NC_000913/4083766‑4083951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`