Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,103,519 +T intergenic (‑268/‑530) cpxR ← / → yiiP transcriptional regulator in 2‑component system/putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5191.T100.0% 854.4 / NA 234intergenic (‑268/‑530)cpxR/yiiPtranscriptional regulator in 2‑component system/putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (104/130);  total (104/130)

Only 100 of 283 total aligned reads displayed.

GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑G‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605
                                                                                              |                                                                                      
 gCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGcgca‑g‑a                                                                                       >  1:2814170/1‑93 (MQ=255)
  ccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgca‑gta                                                                                       <  2:1558930/93‑3 (MQ=255)
  ccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgca‑gta                                                                                       <  2:1081115/93‑3 (MQ=255)
   cTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgca‑gtac                                                                                      <  2:1138058/93‑4 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgca‑gtacg                                                                                     >  2:443382/1‑89 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgca‑gtacc                                                                                     >  2:3971833/1‑89 (MQ=255)
      caCGGTGGCAGTCAGTTCATTAAGCCCCGCTGCTGAAGTCAGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgca‑gtacgca                                                                                   <  1:385295/92‑1 (MQ=255)
      aaCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgca‑gtacgca                                                                                   <  1:1873587/93‑1 (MQ=255)
        cGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgca‑gtacgcaga                                                                                 <  2:3391383/93‑1 (MQ=255)
        cGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgca‑gtacgcaga                                                                                 <  1:607209/93‑1 (MQ=255)
         gCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCagag                                                                                >  2:925024/1‑93 (MQ=255)
          cTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGc                                                                               <  1:973438/93‑1 (MQ=255)
            ggCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCa                                                                             <  2:4323429/93‑1 (MQ=255)
             gCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCat                                                                            <  1:3498211/93‑1 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCata                                                                           >  2:1393947/1‑93 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCata                                                                           <  1:2524316/93‑1 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCata                                                                           <  1:1060725/93‑1 (MQ=255)
                gtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATg                                                                         <  2:1644841/93‑1 (MQ=255)
                gtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATg                                                                         >  2:4379882/1‑93 (MQ=255)
                gtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATg                                                                         >  2:3002682/1‑93 (MQ=255)
                gtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATg                                                                         >  1:3787129/1‑93 (MQ=255)
                 tcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGt                                                                        >  1:3050648/1‑93 (MQ=255)
                 tcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGt                                                                        >  1:585842/1‑93 (MQ=255)
                 tcagtTCATTAAGCCACGCTGCTGAAGTCGGTTC‑GGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGtt                                                                       <  2:2053625/93‑1 (MQ=255)
                  cagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAATTTACGCAGCGCA‑GTACGCAGAGCCATATGtt                                                                       <  2:676011/93‑1 (MQ=255)
                  cagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGtt                                                                       <  1:834284/93‑1 (MQ=255)
                    gtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCg                                                                     >  2:1512442/1‑93 (MQ=255)
                    gtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCg                                                                     >  1:2750279/1‑93 (MQ=255)
                    gtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCg                                                                     >  1:3629985/1‑93 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGa                                                                    >  2:3609758/1‑93 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGa                                                                    <  2:3277371/93‑1 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGa                                                                    <  2:578377/93‑1 (MQ=255)
                      tCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGAc                                                                   >  2:1874524/1‑93 (MQ=255)
                       cATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACg                                                                  <  2:4216005/93‑1 (MQ=255)
                        aTTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCAGGTGAAGAAATTACGCCGCGCACT‑ACGCAGAGCCATCTGTTCGAccg                                                                 >  2:1490560/1‑91 (MQ=255)
                         ttAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGc                                                                <  1:1245231/93‑1 (MQ=255)
                          tAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCa                                                               >  1:361808/1‑93 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATaa                                                            >  1:4425147/1‑93 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATaa                                                            >  1:4630422/1‑93 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATaa                                                            <  1:1016627/93‑1 (MQ=255)
                              ccACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAg                                                           >  2:2345790/1‑93 (MQ=255)
                               cACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGt                                                          >  1:1194208/1‑93 (MQ=255)
                               cACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGt                                                          <  2:393615/93‑1 (MQ=255)
                                aCGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGtt                                                         <  2:2204210/93‑1 (MQ=255)
                                 cGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGttt                                                        <  2:1807264/93‑1 (MQ=255)
                                   ctgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTaa                                                      <  2:4195113/93‑1 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     <  1:3246053/93‑1 (MQ=255)
                                     gctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAcc                                                    <  1:2186141/93‑1 (MQ=255)
                                      ctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCg                                                   >  1:4139651/1‑93 (MQ=255)
                                      ctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCg                                                   <  2:833362/93‑1 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  2:3553689/1‑93 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  2:184258/1‑93 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  1:3705912/1‑93 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  1:816536/1‑93 (MQ=255)
                                           gTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAt                                              <  1:3944781/93‑1 (MQ=255)
                                            tCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATc                                             <  2:1214534/93‑1 (MQ=255)
                                            tCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATc                                             >  2:3055725/1‑93 (MQ=255)
                                            tCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGAGCC‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACAGAACATc                                             >  2:3111276/1‑93 (MQ=255)
                                             cGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCa                                            <  1:1421895/93‑1 (MQ=255)
                                             cGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCa                                            <  1:2078048/93‑1 (MQ=255)
                                              ggTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAg                                           <  2:728954/93‑1 (MQ=255)
                                                  cAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTc                                       <  2:4119246/93‑1 (MQ=255)
                                                   aGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTca                                      >  2:2839077/1‑93 (MQ=255)
                                                    ggCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcag                                     >  1:2380284/1‑93 (MQ=255)
                                                     gCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagc                                    <  1:2703625/93‑1 (MQ=255)
                                                      cGATAACTGTCATCCGGTTGAAGAACTTACGCAGCACA‑GTCCGCAGAGCCATATGTTCGACGTCATAATTTTAACCGATCATCAGCGTcagca                                   <  2:2314695/93‑1 (MQ=255)
                                                       gATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagcag                                  >  1:3568933/1‑93 (MQ=255)
                                                        aTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa                                 <  2:2126928/93‑1 (MQ=255)
                                                        aTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa                                 <  1:1272616/93‑1 (MQ=255)
                                                         tAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAt                                <  2:1741528/93‑1 (MQ=255)
                                                          aaCTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATg                               <  1:3996011/93‑1 (MQ=255)
                                                           aCTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGc                              <  1:2560497/93‑1 (MQ=255)
                                                           aCTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGc                              <  1:1564405/93‑1 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            <  1:531491/93‑1 (MQ=255)
                                                              ggCATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgag                           <  2:3771200/93‑1 (MQ=255)
                                                               gCATCCGGGGGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgaga                          >  1:3394400/1‑93 (MQ=255)
                                                                cATCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGAt                         <  1:1763959/93‑1 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        <  2:2796067/93‑1 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        <  1:4212547/93‑1 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        <  2:545893/93‑1 (MQ=255)
                                                                   ccGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCtt                      <  2:1708613/93‑1 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     >  1:2946314/1‑93 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCA‑GTAAGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     <  1:317556/93‑1 (MQ=255)
                                                                     gggTGAAGAACTTACGCAGCGCA‑GTACGCAGAG‑CATATGTTCGACGGCATAAGTTTAAACGAACATCAGCGGCAGCAGATGGGGGGTTTTATg                   >  2:3270098/1‑93 (MQ=255)
                                                                          aagaaCTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               >  1:1344978/1‑93 (MQ=255)
                                                                            gaaCTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             <  2:3491596/93‑1 (MQ=255)
                                                                            gaaCTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             <  2:3727547/93‑1 (MQ=255)
                                                                            gaaCTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             <  1:4073047/93‑1 (MQ=255)
                                                                            gaaCGTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             <  1:2349845/93‑1 (MQ=255)
                                                                             aaCTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAgg            <  2:615845/93‑1 (MQ=255)
                                                                             aaCTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAgg            >  2:2406507/1‑93 (MQ=255)
                                                                             aaCTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAgg            >  1:1718264/1‑93 (MQ=255)
                                                                              aCTTACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGc           >  2:3841595/1‑93 (MQ=255)
                                                                                ttACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGccc         >  2:2701244/1‑93 (MQ=255)
                                                                                 tACGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCg        >  2:2676158/1‑93 (MQ=255)
                                                                                  aCGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCgg       <  1:1646293/93‑1 (MQ=255)
                                                                                  aCGCAGCGCA‑GTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATACGAGATCTTATGCAACAGGCCCgg       >  2:4597614/1‑93 (MQ=255)
                                                                                    gcagcgca‑gtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     <  1:1993004/93‑1 (MQ=255)
                                                                                      agcgca‑gtACGCACAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACg   <  2:4141238/93‑1 (MQ=255)
                                                                                       gcgca‑gtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGa  <  2:783844/93‑1 (MQ=255)
                                                                                              |                                                                                      
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCA‑G‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCT  >  NC_000913/4103427‑4103612
                                                                                            |                                                                                              
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑T                                                                                              <  SRR4302535.169841/94‑1 (MQ=60)
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑T                                                                                              <  SRR4302535.4307882/94‑1 (MQ=60)
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑A                                                                                              >  SRR4302535.2834169/1‑94 (MQ=60)
  CCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTAGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGtag                                                                                             >  SRR4302535.3316389/1‑91 (MQ=60)
   CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                            >  SRR4302535.1882727/1‑94 (MQ=60)
    TCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGC                                                                                           >  SRR4302535.2099747/1‑94 (MQ=60)
     CAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCA                                                                                          <  SRR4302535.1350903/94‑1 (MQ=60)
     CAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCA                                                                                          >  SRR4302535.3636781/1‑94 (MQ=60)
      cacggTGGCAGTCAGTTCATTAAGCCCCGCTGCTGAAGTCAGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                         <  SRR4302535.387971/89‑1 (MQ=60)
      AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                         <  SRR4302535.1887089/94‑1 (MQ=60)
      AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                         >  SRR4302535.1568873/1‑94 (MQ=60)
        CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                       <  SRR4302535.175574/94‑1 (MQ=60)
        CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                       <  SRR4302535.611447/94‑1 (MQ=60)
        CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAC                                                                                       >  SRR4302535.827809/1‑94 (MQ=60)
         GCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGC                                                                                      >  SRR4302535.1247135/1‑94 (MQ=60)
         GCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGC                                                                                      >  SRR4302535.1578007/1‑94 (MQ=60)
          CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                     <  SRR4302535.980347/94‑1 (MQ=60)
           TGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCA                                                                                    >  SRR4302535.2813603/1‑94 (MQ=60)
            GGCAGTCAGTTCATGAAGCCACGCTGCTGAAGTCGGTTCAGGCGAGAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCAT                                                                                   <  SRR4302535.1565612/94‑1 (MQ=60)
             GCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATA                                                                                  <  SRR4302535.3522517/94‑1 (MQ=60)
              CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                                 <  SRR4302535.2542504/94‑1 (MQ=60)
              CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                                 <  SRR4302535.1068277/94‑1 (MQ=60)
              CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                                 >  SRR4302535.2394657/1‑94 (MQ=60)
               AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                                >  SRR4302535.3813254/1‑94 (MQ=60)
                GTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGT                                                                               >  SRR4302535.3072017/1‑94 (MQ=60)
                GTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGT                                                                               >  SRR4302535.589946/1‑94 (MQ=60)
                GTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGT                                                                               <  SRR4302535.3646664/94‑1 (MQ=60)
                 TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                              >  SRR4302535.412389/1‑94 (MQ=60)
                 TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                              <  SRR4302535.2740613/94‑1 (MQ=60)
                  CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             <  SRR4302535.840134/94‑1 (MQ=60)
                   AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                            >  SRR4302535.3655145/1‑94 (MQ=60)
                   AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                            >  SRR4302535.3254948/1‑94 (MQ=60)
                   AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                            >  SRR4302535.2769886/1‑94 (MQ=60)
                   AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                            >  SRR4302535.107893/1‑94 (MQ=60)
                    GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                           >  SRR4302535.605163/1‑94 (MQ=60)
                     TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                          <  SRR4302535.2138694/94‑1 (MQ=60)
                     TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                          <  SRR4302535.2743641/94‑1 (MQ=60)
                      TCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACG                                                                         >  SRR4302535.1778769/1‑94 (MQ=60)
                       CATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTTGAAGG                                                                        >  SRR4302535.2505814/1‑94 (MQ=60)
                       CATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGG                                                                        <  SRR4302535.3789262/94‑1 (MQ=60)
                       CATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGG                                                                        <  SRR4302535.606161/94‑1 (MQ=60)
                         TTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCA                                                                      >  SRR4302535.364305/1‑94 (MQ=60)
                         TTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCA                                                                      <  SRR4302535.1254115/94‑1 (MQ=60)
                            AGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAA                                                                   >  SRR4302535.4661840/1‑94 (MQ=60)
                            AGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAA                                                                   >  SRR4302535.4455457/1‑94 (MQ=60)
                            AGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAA                                                                   >  SRR4302535.4663251/1‑94 (MQ=60)
                             GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                                  <  SRR4302535.1023852/94‑1 (MQ=60)
                             GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                                  <  SRR4302535.4055537/94‑1 (MQ=60)
                              CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                                 >  SRR4302535.2544321/1‑94 (MQ=60)
                              CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                                 <  SRR4302535.3737011/94‑1 (MQ=60)
                              CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                                 >  SRR4302535.1202763/1‑94 (MQ=60)
                               CACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTT                                                                >  SRR4302535.3896605/1‑94 (MQ=60)
                               CACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTT                                                                >  SRR4302535.4145825/1‑94 (MQ=60)
                               CACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTT                                                                >  SRR4302535.3613904/1‑94 (MQ=60)
                               CACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCAGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTT                                                                >  SRR4302535.835997/1‑94 (MQ=60)
                                   CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                            <  SRR4302535.2849283/94‑1 (MQ=60)
                                   CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGCTTAAC                                                            >  SRR4302535.1641742/1‑94 (MQ=60)
                                    TGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACC                                                           <  SRR4302535.3268596/94‑1 (MQ=60)
                                     GCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCG                                                          >  SRR4302535.4167998/1‑94 (MQ=60)
                                     GCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCG                                                          <  SRR4302535.2201718/94‑1 (MQ=60)
                                      CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                         >  SRR4302535.3731575/1‑94 (MQ=60)
                                      CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                         >  SRR4302535.822261/1‑94 (MQ=60)
                                       TGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAA                                                        >  SRR4302535.2339564/1‑94 (MQ=60)
                                        GAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAC                                                       >  SRR4302535.913889/1‑94 (MQ=60)
                                         AAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACA                                                      <  SRR4302535.1949149/94‑1 (MQ=60)
                                          AGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAT                                                     >  SRR4302535.1164976/1‑94 (MQ=60)
                                          AGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAT                                                     <  SRR4302535.878436/94‑1 (MQ=60)
                                           GTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATC                                                    <  SRR4302535.2956052/94‑1 (MQ=60)
                                           GTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATC                                                    <  SRR4302535.3971718/94‑1 (MQ=60)
                                           GTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATC                                                    <  SRR4302535.3078483/94‑1 (MQ=60)
                                            TCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAGCATCA                                                   <  SRR4302535.4602028/94‑1 (MQ=60)
                                             CGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAG                                                  >  SRR4302535.369323/1‑94 (MQ=60)
                                             CGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAG                                                  <  SRR4302535.2093019/94‑1 (MQ=60)
                                             CGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAG                                                  <  SRR4302535.1432046/94‑1 (MQ=60)
                                              GGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGC                                                 <  SRR4302535.806014/94‑1 (MQ=60)
                                               GTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCG                                                <  SRR4302535.3499405/94‑1 (MQ=60)
                                               GTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCG                                                >  SRR4302535.4168305/1‑94 (MQ=60)
                                                TTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGT                                               <  SRR4302535.2126799/94‑1 (MQ=60)
                                                  CAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCA                                             >  SRR4302535.4535203/1‑94 (MQ=60)
                                                   AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                            >  SRR4302535.2431101/1‑94 (MQ=60)
                                                   AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                            >  SRR4302535.2397377/1‑94 (MQ=60)
                                                    GGCGATAACTGGCATCCGGGTGAAGAACTTCCGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGC                                           <  SRR4302535.2989532/94‑1 (MQ=60)
                                                    GGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGC                                           <  SRR4302535.2978299/94‑1 (MQ=60)
                                                     GCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCA                                          <  SRR4302535.2722940/94‑1 (MQ=60)
                                                      CGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAG                                         >  SRR4302535.3593683/1‑94 (MQ=60)
                                                        ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                       <  SRR4302535.1326769/94‑1 (MQ=60)
                                                        ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                       <  SRR4302535.1414446/94‑1 (MQ=60)
                                                        ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                       >  SRR4302535.4710678/1‑94 (MQ=60)
                                                        ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                       >  SRR4302535.1259307/1‑94 (MQ=60)
                                                        ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                       <  SRR4302535.1281708/94‑1 (MQ=60)
                                                          AACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGC                                     <  SRR4302535.4023352/94‑1 (MQ=60)
                                                           ACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCG                                    <  SRR4302535.2578930/94‑1 (MQ=60)
                                                           ACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCG                                    <  SRR4302535.1575697/94‑1 (MQ=60)
                                                           ACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCG                                    <  SRR4302535.398554/94‑1 (MQ=60)
                                                             TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  <  SRR4302535.1744282/94‑1 (MQ=60)
                                                             TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  <  SRR4302535.237992/94‑1 (MQ=60)
                                                             TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  >  SRR4302535.3698323/1‑94 (MQ=60)
                                                             TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  <  SRR4302535.121512/94‑1 (MQ=60)
                                                             TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  <  SRR4302535.535209/94‑1 (MQ=60)
                                                              GGCATCCGGGGGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGA                                 >  SRR4302535.3418016/1‑94 (MQ=60)
                                                               GCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAGCATCAGCGTCAGCAGATGCGAGAT                                <  SRR4302535.2054954/94‑1 (MQ=60)
                                                                CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                               <  SRR4302535.1776741/94‑1 (MQ=60)
                                                                 ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                              >  SRR4302535.3332241/1‑94 (MQ=60)
                                                                 ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                              >  SRR4302535.1290956/1‑94 (MQ=60)
                                                                 ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                              <  SRR4302535.4241378/94‑1 (MQ=60)
                                                                   CCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTA                            >  SRR4302535.2967019/1‑94 (MQ=60)
                                                                   CCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTA                            <  SRR4302535.4211074/94‑1 (MQ=60)
                                                                    CGGGTGAAGAACTTACGCAGCGCAGTAAGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                           <  SRR4302535.319760/94‑1 (MQ=60)
                                                                     GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                          >  SRR4302535.1273489/1‑94 (MQ=60)
                                                                       GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                        >  SRR4302535.3210863/1‑94 (MQ=60)
                                                                       GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                        <  SRR4302535.2241987/94‑1 (MQ=60)
                                                                         GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      <  SRR4302535.3856939/94‑1 (MQ=60)
                                                                         GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      >  SRR4302535.1354580/1‑94 (MQ=60)
                                                                         GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      <  SRR4302535.749888/94‑1 (MQ=60)
                                                                         GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      >  SRR4302535.3999036/1‑94 (MQ=60)
                                                                          AAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACA                     <  SRR4302535.2053198/94‑1 (MQ=60)
                                                                          AAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACA                     >  SRR4302535.3844085/1‑94 (MQ=60)
                                                                           AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                    <  SRR4302535.3918867/94‑1 (MQ=60)
                                                                            GAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                   <  SRR4302535.4100918/94‑1 (MQ=60)
                                                                            GAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                   >  SRR4302535.1730694/1‑94 (MQ=60)
                                                                            GAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGC                   >  SRR4302535.545276/1‑94 (MQ=60)
                                                                            GAACGTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                   <  SRR4302535.2366692/94‑1 (MQ=60)
                                                                            AAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                   <  SRR4302535.4366651/94‑1 (MQ=60)
                                                                             AACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGC                  <  SRR4302535.2661877/94‑1 (MQ=60)
                                                                              ACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCC                 <  SRR4302535.2719175/94‑1 (MQ=60)
                                                                              ACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCC                 >  SRR4302535.3528714/1‑94 (MQ=60)
                                                                                 TACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGG              <  SRR4302535.1718397/94‑1 (MQ=60)
                                                                                  ACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGC             <  SRR4302535.1658191/94‑1 (MQ=60)
                                                                                   CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA            >  SRR4302535.1064885/1‑94 (MQ=60)
                                                                                    GCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAC           <  SRR4302535.2007355/94‑1 (MQ=60)
                                                                                     CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG          >  SRR4302535.1552959/1‑94 (MQ=60)
                                                                                     CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG          <  SRR4302535.2784098/94‑1 (MQ=60)
                                                                                       GCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAA        >  SRR4302535.2737407/1‑94 (MQ=60)
                                                                                       GCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAA        <  SRR4302535.3524274/94‑1 (MQ=60)
                                                                                       GCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAA        <  SRR4302535.3515095/94‑1 (MQ=60)
                                                                                        CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC       <  SRR4302535.1330636/94‑1 (MQ=60)
                                                                                        CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC       <  SRR4302535.332852/94‑1 (MQ=60)
                                                                                          CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     <  SRR4302535.52559/94‑1 (MQ=60)
                                                                                          CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     >  SRR4302535.4640075/1‑94 (MQ=60)
                                                                                          CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     <  SRR4302535.2700570/94‑1 (MQ=60)
                                                                                          CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     >  SRR4302535.869285/1‑94 (MQ=60)
                                                                                          CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     >  SRR4302535.1119127/1‑94 (MQ=60)
                                                                                            T‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCC  >  SRR4302535.2680521/1‑94 (MQ=60)
                                                                                            |                                                                                              
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCT  >  NC_000913/4103427‑4103612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑