Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,108,133 A→G G208G (GGA→GGG)  cdh → CDP‑diacylglycerol phosphotidylhydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,108,1330AG100.0% 717.3 / NA 227G208G (GGA→GGG) cdhCDP‑diacylglycerol phosphotidylhydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (103/124);  total (103/124)

Only 100 of 238 total aligned reads displayed.

GCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTC  >  NC_000913/4108044‑4108222
                                                                                         |                                                                                         
  gcgTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTa                                                                                      >  2:1012466/1‑93 (MQ=255)
     tCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACgg                                                                                   >  1:188228/1‑93 (MQ=255)
     gcGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACgg                                                                                   <  2:1578698/92‑1 (MQ=255)
      cGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACggg                                                                                  >  1:4575508/1‑93 (MQ=255)
       gggTAACGGAAGGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGc                                                                                 <  1:3913032/93‑1 (MQ=255)
       gggTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGc                                                                                 <  1:1857121/93‑1 (MQ=255)
       gggTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGc                                                                                 <  1:2841963/93‑1 (MQ=255)
        ggTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCt                                                                                <  1:3220501/93‑1 (MQ=255)
        ggTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCt                                                                                <  2:4674579/93‑1 (MQ=255)
         gTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTg                                                                               <  1:251179/93‑1 (MQ=255)
         gTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTg                                                                               <  2:1637547/93‑1 (MQ=255)
         gTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTg                                                                               <  1:2324544/93‑1 (MQ=255)
          tAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGCACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTgg                                                                              <  1:868851/93‑1 (MQ=255)
           aaCGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGc                                                                             <  1:3523699/93‑1 (MQ=255)
             cGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGa                                                                           >  2:3323504/1‑93 (MQ=255)
             cGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGa                                                                           <  1:2566778/93‑1 (MQ=255)
              ggAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGAt                                                                          <  2:3740740/93‑1 (MQ=255)
               gAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATg                                                                         <  2:1160156/93‑1 (MQ=255)
               gAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATg                                                                         <  1:2028049/93‑1 (MQ=255)
               gAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATg                                                                         >  2:450321/1‑93 (MQ=255)
                aaaGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGTAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATgg                                                                        <  2:558701/93‑1 (MQ=255)
                   gCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTAc                                                                     <  2:3639322/93‑1 (MQ=255)
                    cGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGAGCTACGGGCTGGCGATGGTACg                                                                    >  1:1460571/1‑93 (MQ=255)
                     gAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACgg                                                                   <  2:2070246/93‑1 (MQ=255)
                     gAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACgg                                                                   >  1:485618/1‑93 (MQ=255)
                      aaCTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGc                                                                  <  2:652025/93‑1 (MQ=255)
                       aCTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCa                                                                 <  1:2997130/93‑1 (MQ=255)
                        cTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCag                                                                <  2:3001641/93‑1 (MQ=255)
                        cTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCag                                                                >  1:1075250/1‑93 (MQ=255)
                         tGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCaga                                                               <  1:552935/93‑1 (MQ=255)
                         tGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCaga                                                               >  2:4133797/1‑93 (MQ=255)
                           gTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCagagg                                                             >  2:1203354/1‑92 (MQ=255)
                            tACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTg                                                            >  1:4254202/1‑93 (MQ=255)
                              cAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGAt                                                          <  2:3077109/93‑1 (MQ=255)
                              cAACGCACCCCATTTACGATGCTGGCAGAAGAAGTCCCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGAt                                                          <  1:1201512/93‑1 (MQ=255)
                               agcGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATa                                                         <  1:1685358/91‑1 (MQ=255)
                               aaCGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATa                                                         >  1:3181772/1‑93 (MQ=255)
                                aCGCAGCCCATTTATGATGCTCGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATaa                                                        <  2:4435784/93‑1 (MQ=255)
                                    aGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCa                                                    >  1:1192247/1‑93 (MQ=255)
                                     gCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAt                                                   >  2:4010146/1‑93 (MQ=255)
                                     gCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAt                                                   <  1:4099707/93‑1 (MQ=255)
                                      cccATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAtt                                                  <  1:2160627/93‑1 (MQ=255)
                                      cccATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAtt                                                  >  1:1692380/1‑93 (MQ=255)
                                       ccATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAttt                                                 <  1:1990243/93‑1 (MQ=255)
                                       ccATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAttt                                                 <  1:2390005/93‑1 (MQ=255)
                                        cATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTg                                                >  1:1774048/1‑93 (MQ=255)
                                        cATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTg                                                <  1:2809290/93‑1 (MQ=255)
                                        cATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGGGATAACTCATTTg                                                >  2:2702952/1‑93 (MQ=255)
                                         aTTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGt                                               >  1:2147171/1‑93 (MQ=255)
                                         aTTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGt                                               >  1:510993/1‑93 (MQ=255)
                                         aTTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGt                                               <  1:2690784/93‑1 (MQ=255)
                                          tttATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTa                                              <  1:274934/93‑1 (MQ=255)
                                           ttATGATGCTGTCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAt                                             <  2:2723492/93‑1 (MQ=255)
                                           ttATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAt                                             >  2:909586/1‑93 (MQ=255)
                                           ttATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAt                                             >  2:1692852/1‑93 (MQ=255)
                                             atgatgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTg                                           >  1:87965/1‑93 (MQ=255)
                                             atgatgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTg                                           >  1:3658515/1‑93 (MQ=255)
                                               gatgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCt                                         <  2:2211502/93‑1 (MQ=255)
                                                atgCTGGAAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTg                                        <  1:458003/93‑1 (MQ=255)
                                                 tgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGGGTGACAACTCATTTGTATTTCTgg                                       >  2:3891117/1‑93 (MQ=255)
                                                 tgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTgg                                       <  1:587046/93‑1 (MQ=255)
                                                 tgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTgg                                       >  1:4449892/1‑93 (MQ=255)
                                                 tgCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTgg                                       >  2:1836906/1‑93 (MQ=255)
                                                  gCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGGGATAACTCATTTGTATTGCTGGc                                      >  1:942108/1‑93 (MQ=255)
                                                    tGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGa                                    >  1:417564/1‑93 (MQ=255)
                                                    tGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGa                                    >  1:662094/1‑93 (MQ=255)
                                                     ggCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGac                                   >  1:4017941/1‑93 (MQ=255)
                                                        agaagaAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGAcaca                                >  1:1386280/1‑93 (MQ=255)
                                                         gaagaaGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACaa                               >  2:4625033/1‑93 (MQ=255)
                                                          aagaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAAc                              <  2:4107194/93‑1 (MQ=255)
                                                          aagaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAAc                              >  2:1144543/1‑93 (MQ=255)
                                                          aagaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAAc                              <  1:290210/93‑1 (MQ=255)
                                                           agaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACg                             >  2:3447228/1‑93 (MQ=255)
                                                            gaagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGAAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGa                            <  1:3597396/93‑1 (MQ=255)
                                                            gaagTACCTGAGGCGCGGGAACAGATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGa                            <  2:238997/93‑1 (MQ=255)
                                                             aagTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGaa                           <  2:313143/93‑1 (MQ=255)
                                                              agTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGaaa                          <  1:3375483/93‑1 (MQ=255)
                                                              agTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGaaa                          <  2:696071/93‑1 (MQ=255)
                                                              agTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTACTGGCGACACAACaaaa                          >  2:4538067/1‑93 (MQ=255)
                                                               gTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAt                         <  1:1233507/93‑1 (MQ=255)
                                                               gTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAt                         <  1:1108603/93‑1 (MQ=255)
                                                                tACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATc                        <  2:2244392/93‑1 (MQ=255)
                                                                tACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATc                        <  1:1006082/93‑1 (MQ=255)
                                                                 aCCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATCTGTATTGCTGGCGACACAACGAAATCt                       >  2:3580562/1‑93 (MQ=255)
                                                                  ccTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGGACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTa                      >  2:4317690/1‑93 (MQ=255)
                                                                   cTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACTGCAGAGTGATAACTCATTTGTATTGCTGGCGGCACAACGAAATCTAc                     >  1:1638135/1‑93 (MQ=255)
                                                                   cTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAc                     <  1:4300707/93‑1 (MQ=255)
                                                                    tGAGGCGCGGGAACATGTGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACt                    >  2:2513624/1‑93 (MQ=255)
                                                                       ggCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGag                 >  1:3530247/1‑92 (MQ=255)
                                                                         cgcgGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGc               <  2:2946468/93‑1 (MQ=255)
                                                                          gcgGGAACATATGGGGCGCTACGGACTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCt              <  2:2874343/93‑1 (MQ=255)
                                                                            gggAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTa            <  1:3355925/93‑1 (MQ=255)
                                                                             ggAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTaa           <  1:3050702/93‑1 (MQ=255)
                                                                              gAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAAc          >  2:2405699/1‑93 (MQ=255)
                                                                              gAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAAc          <  1:3105130/93‑1 (MQ=255)
                                                                                 cATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGt       <  2:2838726/93‑1 (MQ=255)
                                                                                  atatGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTg      >  1:3423846/1‑93 (MQ=255)
                                                                                   tatGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGc     <  2:1385115/93‑1 (MQ=255)
                                                                                    atGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCt    <  2:1933890/93‑1 (MQ=255)
                                                                                      ggggCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTc  <  2:534542/93‑1 (MQ=255)
                                                                                         |                                                                                         
GCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTC  >  NC_000913/4108044‑4108222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCC  >  NC_000913/4108040‑4108226
                                                                                             |                                                                                             
CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGG                                                                                               >  SRR4302535.1724240/1‑94 (MQ=60)
CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGG                                                                                               >  SRR4302535.3870204/1‑94 (MQ=60)
    GCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCT                                                                                           <  SRR4302535.2095008/94‑1 (MQ=60)
     CGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTC                                                                                          >  SRR4302535.2671351/1‑94 (MQ=60)
        GTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGG                                                                                       >  SRR4302535.189531/1‑94 (MQ=60)
         TCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGG                                                                                      >  SRR4302535.4606688/1‑94 (MQ=60)
           GGGTAACGGAAGGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCT                                                                                    <  SRR4302535.3939817/94‑1 (MQ=60)
           GGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCT                                                                                    <  SRR4302535.2862120/94‑1 (MQ=60)
           GGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCT                                                                                    <  SRR4302535.3156052/94‑1 (MQ=60)
           GGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCT                                                                                    <  SRR4302535.1870495/94‑1 (MQ=60)
            GGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTG                                                                                   >  SRR4302535.3949550/1‑94 (MQ=60)
            GGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTG                                                                                   <  SRR4302535.3242818/94‑1 (MQ=60)
            GGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTG                                                                                   <  SRR4302535.525289/94‑1 (MQ=60)
             GTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGG                                                                                  <  SRR4302535.2341176/94‑1 (MQ=60)
             GTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGG                                                                                  <  SRR4302535.252908/94‑1 (MQ=60)
              TAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGC                                                                                 <  SRR4302535.4698022/94‑1 (MQ=60)
              TAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGCACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGC                                                                                 <  SRR4302535.875001/94‑1 (MQ=60)
               AACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCG                                                                                <  SRR4302535.125105/94‑1 (MQ=60)
               AACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCG                                                                                >  SRR4302535.2361441/1‑94 (MQ=60)
               AACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCG                                                                                <  SRR4302535.3548169/94‑1 (MQ=60)
                 CGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGAT                                                                              >  SRR4302535.2005972/1‑94 (MQ=60)
                 CGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGAT                                                                              <  SRR4302535.2585244/94‑1 (MQ=60)
                  GGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATG                                                                             <  SRR4302535.1884576/94‑1 (MQ=60)
                  GGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATG                                                                             <  SRR4302535.4027681/94‑1 (MQ=60)
                   GAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGG                                                                            <  SRR4302535.2042670/94‑1 (MQ=60)
                    AAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGT                                                                           >  SRR4302535.3071028/1‑94 (MQ=60)
                    AAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGT                                                                           >  SRR4302535.997102/1‑94 (MQ=60)
                    AAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGT                                                                           <  SRR4302535.1240057/94‑1 (MQ=60)
                      AGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTAC                                                                         <  SRR4302535.3592946/94‑1 (MQ=60)
                      AGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTAC                                                                         <  SRR4302535.4274014/94‑1 (MQ=60)
                       GCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGAGCTACGGGCTGGCGATGGTACG                                                                        >  SRR4302535.1471036/1‑94 (MQ=60)
                        CGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGG                                                                       >  SRR4302535.489023/1‑94 (MQ=60)
                        CGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGG                                                                       >  SRR4302535.196304/1‑94 (MQ=60)
                        CGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGG                                                                       <  SRR4302535.348928/94‑1 (MQ=60)
                           ACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAG                                                                    >  SRR4302535.1199855/1‑94 (MQ=60)
                           ACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAG                                                                    >  SRR4302535.1082892/1‑94 (MQ=60)
                           ACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAG                                                                    >  SRR4302535.523353/1‑94 (MQ=60)
                           ACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAG                                                                    <  SRR4302535.3018148/94‑1 (MQ=60)
                             TGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAG                                                                  <  SRR4302535.556806/94‑1 (MQ=60)
                             TGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAG                                                                  <  SRR4302535.889263/94‑1 (MQ=60)
                               GTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTG                                                                >  SRR4302535.4283349/1‑94 (MQ=60)
                                TACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGA                                                               >  SRR4302535.429560/1‑94 (MQ=60)
                                TACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGA                                                               >  SRR4302535.2841024/1‑94 (MQ=60)
                                 ACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGAT                                                              >  SRR4302535.1183532/1‑94 (MQ=60)
                                 ACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGAT                                                              >  SRR4302535.1113400/1‑94 (MQ=60)
                                  CAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATA                                                             >  SRR4302535.3203763/1‑94 (MQ=60)
                                  CAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATA                                                             >  SRR4302535.1202318/1‑94 (MQ=60)
                                  CAACGCACCCCATTTACGATGCTGGCAGAAGAAGTCCCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATA                                                             <  SRR4302535.1210117/94‑1 (MQ=60)
                                   AGCGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAA                                                            <  SRR4302535.1697562/94‑1 (MQ=60)
                                    ACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAAC                                                           >  SRR4302535.885811/1‑94 (MQ=60)
                                    ACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAAC                                                           <  SRR4302535.4457708/94‑1 (MQ=60)
                                       CAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCA                                                        >  SRR4302535.1200785/1‑94 (MQ=60)
                                        AGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCAT                                                       <  SRR4302535.1623848/94‑1 (MQ=60)
                                         GCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATT                                                      <  SRR4302535.4127777/94‑1 (MQ=60)
                                         GCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATT                                                      >  SRR4302535.1704621/1‑94 (MQ=60)
                                          CCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTT                                                     <  SRR4302535.2543639/94‑1 (MQ=60)
                                          CCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTT                                                     <  SRR4302535.2176140/94‑1 (MQ=60)
                                          CCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTT                                                     <  SRR4302535.3097241/94‑1 (MQ=60)
                                           CCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTG                                                    <  SRR4302535.2082784/94‑1 (MQ=60)
                                           CCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTG                                                    <  SRR4302535.2407190/94‑1 (MQ=60)
                                           CCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTG                                                    <  SRR4302535.2004574/94‑1 (MQ=60)
                                           CCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTG                                                    >  SRR4302535.1786901/1‑94 (MQ=60)
                                            CATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGT                                                   <  SRR4302535.2829261/94‑1 (MQ=60)
                                            CATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGT                                                   >  SRR4302535.2162616/1‑94 (MQ=60)
                                            CATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGT                                                   <  SRR4302535.1716853/94‑1 (MQ=60)
                                            CATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGT                                                   >  SRR4302535.514568/1‑94 (MQ=60)
                                             ATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTA                                                  <  SRR4302535.2710016/94‑1 (MQ=60)
                                             ATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTA                                                  >  SRR4302535.3291057/1‑94 (MQ=60)
                                             ATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCCGGCGATGGTACGGCAGAGTGATAACTCATTTGTA                                                  <  SRR4302535.2710017/94‑1 (MQ=60)
                                              TTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAT                                                 <  SRR4302535.276847/94‑1 (MQ=60)
                                              TTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAT                                                 <  SRR4302535.4149130/94‑1 (MQ=60)
                                              TTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAT                                                 <  SRR4302535.4429552/94‑1 (MQ=60)
                                              TTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTAT                                                 <  SRR4302535.3240412/94‑1 (MQ=60)
                                               TTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATT                                                >  SRR4302535.3089720/1‑94 (MQ=60)
                                                TATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTG                                               <  SRR4302535.3255307/94‑1 (MQ=60)
                                                TATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTG                                               >  SRR4302535.3683840/1‑94 (MQ=60)
                                                TATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTG                                               >  SRR4302535.88582/1‑94 (MQ=60)
                                                  TGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCT                                             <  SRR4302535.2654245/94‑1 (MQ=60)
                                                  TGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCT                                             >  SRR4302535.1449439/1‑94 (MQ=60)
                                                   GATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTG                                            <  SRR4302535.3395062/94‑1 (MQ=60)
                                                   GATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTG                                            <  SRR4302535.495/94‑1 (MQ=60)
                                                    ATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGG                                           >  SRR4302535.4480413/1‑94 (MQ=60)
                                                    ATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGG                                           >  SRR4302535.3439165/1‑94 (MQ=60)
                                                    ATGCTGGAAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGG                                           <  SRR4302535.461206/94‑1 (MQ=60)
                                                     TGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGC                                          <  SRR4302535.591158/94‑1 (MQ=60)
                                                     TGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGC                                          >  SRR4302535.4274182/1‑94 (MQ=60)
                                                     TGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGGGATAACTCATTTGTATTGCTGGC                                          >  SRR4302535.948779/1‑94 (MQ=60)
                                                       CTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGA                                        >  SRR4302535.420491/1‑94 (MQ=60)
                                                       CTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGA                                        >  SRR4302535.666714/1‑94 (MQ=60)
                                                        TGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGAC                                       <  SRR4302535.1476192/94‑1 (MQ=60)
                                                        TGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGAC                                       >  SRR4302535.4045429/1‑94 (MQ=60)
                                                         GGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACA                                      <  SRR4302535.4658048/94‑1 (MQ=60)
                                                           CAGAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACA                                    >  SRR4302535.1396174/1‑94 (MQ=60)
                                                             GAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAAC                                  >  SRR4302535.1666879/1‑94 (MQ=60)
                                                             GAAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAAC                                  <  SRR4302535.1831551/94‑1 (MQ=60)
                                                              AAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACG                                 >  SRR4302535.1702250/1‑94 (MQ=60)
                                                              AAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACG                                 >  SRR4302535.1750281/1‑94 (MQ=60)
                                                              AAGAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACG                                 <  SRR4302535.292244/94‑1 (MQ=60)
                                                                GGAGTACCTGAGGCGCGGGAACATATGGGGCTCTACCGGCTGGCGATGGTACGGAAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAA                               <  SRR4302535.3622362/94‑1 (MQ=60)
                                                                GAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAA                               >  SRR4302535.30153/1‑94 (MQ=60)
                                                                GAAGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGAAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAA                               <  SRR4302535.3622361/94‑1 (MQ=60)
                                                                  AGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAT                             <  SRR4302535.3728542/94‑1 (MQ=60)
                                                                  AGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAT                             >  SRR4302535.3583986/1‑94 (MQ=60)
                                                                  AGTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAAT                             <  SRR4302535.3398958/94‑1 (MQ=60)
                                                                   GTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATC                            <  SRR4302535.1116523/94‑1 (MQ=60)
                                                                   GTACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATC                            <  SRR4302535.1242326/94‑1 (MQ=60)
                                                                    TACCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCT                           <  SRR4302535.1013230/94‑1 (MQ=60)
                                                                      CCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACTGCAGAGTGATAACTCATTTGTATTGCTGGCGGCACAACGAAATCTAC                         >  SRR4302535.1649979/1‑94 (MQ=60)
                                                                      CCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAC                         >  SRR4302535.1393349/1‑94 (MQ=60)
                                                                      CCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAC                         >  SRR4302535.872868/1‑94 (MQ=60)
                                                                      CCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAC                         <  SRR4302535.292150/94‑1 (MQ=60)
                                                                      CCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAC                         >  SRR4302535.2281914/1‑94 (MQ=60)
                                                                      CCTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTAC                         <  SRR4302535.3007944/94‑1 (MQ=60)
                                                                       CTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACT                        >  SRR4302535.1055538/1‑94 (MQ=60)
                                                                       CTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACT                        <  SRR4302535.4599002/94‑1 (MQ=60)
                                                                       CTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACT                        <  SRR4302535.4330137/94‑1 (MQ=60)
                                                                       CTGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACT                        >  SRR4302535.4105069/1‑94 (MQ=60)
                                                                        TGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTG                       <  SRR4302535.216915/94‑1 (MQ=60)
                                                                        TGAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTG                       <  SRR4302535.2472279/94‑1 (MQ=60)
                                                                         GAGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGA                      >  SRR4302535.2372/1‑94 (MQ=60)
                                                                          AGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGAG                     >  SRR4302535.3554759/1‑94 (MQ=60)
                                                                          AGGCGCGGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGAC                     >  SRR4302535.2809854/1‑94 (MQ=60)
                                                                             CGCGGTAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCT                  <  SRR4302535.4607705/94‑1 (MQ=60)
                                                                                GGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAA               <  SRR4302535.3379292/94‑1 (MQ=60)
                                                                                GGGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAA               >  SRR4302535.3551195/1‑94 (MQ=60)
                                                                                 GGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAAC              >  SRR4302535.1942270/1‑94 (MQ=60)
                                                                                 GGAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAAC              <  SRR4302535.3072072/94‑1 (MQ=60)
                                                                                  GAACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACC             <  SRR4302535.3126748/94‑1 (MQ=60)
                                                                                    ACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGT           >  SRR4302535.6624/1‑94 (MQ=60)
                                                                                    ACATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGT           <  SRR4302535.85629/94‑1 (MQ=60)
                                                                                     CATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTG          >  SRR4302535.3447658/1‑94 (MQ=60)
                                                                                      ATATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGC         >  SRR4302535.2802206/1‑94 (MQ=60)
                                                                                       TATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACGCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCT        <  SRR4302535.4364071/94‑1 (MQ=60)
                                                                                        ATGGGGCGCTACGGGCTGGCGATTGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTT       <  SRR4302535.567753/94‑1 (MQ=60)
                                                                                        ATGGGGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTT       >  SRR4302535.3450536/1‑94 (MQ=60)
                                                                                            GGCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGC   <  SRR4302535.4582402/94‑1 (MQ=60)
                                                                                             GCGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCC  <  SRR4302535.4393062/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCC  >  NC_000913/4108040‑4108226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑