Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,116,372 T→C intergenic (+32/+53) yiiU → / ← menG hypothetical protein/MenG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,116,3720TC100.0% 748.4 / NA 233intergenic (+32/+53)yiiU/menGhypothetical protein/MenG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (84/149);  total (84/149)

Only 100 of 239 total aligned reads displayed.

AGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATC  >  NC_000913/4116282‑4116461
                                                                                          |                                                                                          
 gcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACcgtg                                                                                         >  1:3790374/1‑93 (MQ=255)
  cagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCgtgt                                                                                        <  2:232788/93‑1 (MQ=255)
   agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                       >  2:680918/1‑93 (MQ=255)
   agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                       <  2:3722308/93‑1 (MQ=255)
    gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAg                                                                                      <  1:2708247/93‑1 (MQ=255)
    gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAg                                                                                      >  2:1617633/1‑93 (MQ=255)
    gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAg                                                                                      >  1:4581164/1‑93 (MQ=255)
    gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAg                                                                                      >  1:886370/1‑93 (MQ=255)
     aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAgg                                                                                     <  1:2966923/93‑1 (MQ=255)
     aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAgg                                                                                     <  1:4421124/93‑1 (MQ=255)
       cGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGcc                                                                                   >  2:1020304/1‑93 (MQ=255)
        ggCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCa                                                                                  >  1:1221467/1‑93 (MQ=255)
         gCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCaa                                                                                 >  1:2894333/1‑93 (MQ=255)
           tGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACa                                                                               >  2:10781/1‑93 (MQ=255)
              cAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGt                                                                            <  2:1425673/93‑1 (MQ=255)
              cAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGt                                                                            <  1:953188/93‑1 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTCCCGTGTAGGCCAACAGGTaa                                                                          >  2:624488/1‑93 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                          <  1:1132925/93‑1 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                          <  2:2591706/93‑1 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                          <  2:436401/93‑1 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                          >  2:823952/1‑93 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                          >  1:1628236/1‑93 (MQ=255)
                 gAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAAc                                                                         <  2:4056612/93‑1 (MQ=255)
                   aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGc                                                                       <  2:1346207/93‑1 (MQ=255)
                   aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGc                                                                       >  2:2305875/1‑93 (MQ=255)
                    cGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCa                                                                      <  2:2731248/93‑1 (MQ=255)
                      tCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑a                                                                   <  2:4188998/93‑1 (MQ=255)
                        tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑aaa                                                                 <  1:1357544/93‑1 (MQ=255)
                        tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑aaa                                                                 <  1:1394759/93‑1 (MQ=255)
                        tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑aaa                                                                 <  2:3755851/93‑1 (MQ=255)
                         gCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑aaaa                                                                <  2:4277783/93‑1 (MQ=255)
                           aGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑aaaaaa                                                              >  1:2523551/1‑93 (MQ=255)
                            ggCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAg                                                             >  2:2147021/1‑93 (MQ=255)
                              cccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGc                                                           <  2:3001338/93‑1 (MQ=255)
                              cccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGc                                                           <  2:3334848/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCt                                                       <  1:175741/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCt                                                       >  2:1478760/1‑93 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCt                                                       <  2:1321579/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCt                                                       <  1:1892011/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCt                                                       <  1:2329391/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCt                                                       <  1:3920093/93‑1 (MQ=255)
                                   ctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCtt                                                      <  1:367954/93‑1 (MQ=255)
                                    tgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTg                                                     <  1:2069859/93‑1 (MQ=255)
                                    tgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTg                                                     >  1:1578065/1‑93 (MQ=255)
                                     gggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGc                                                    <  1:2419653/93‑1 (MQ=255)
                                     gggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGc                                                    <  1:695984/93‑1 (MQ=255)
                                     aggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGc                                                    <  1:414333/92‑1 (MQ=255)
                                        tCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGt                                                 >  2:2204446/1‑93 (MQ=255)
                                          gCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGc                                               >  2:4234164/1‑93 (MQ=255)
                                           cATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGcc                                              <  1:1088379/93‑1 (MQ=255)
                                           cATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGcc                                              >  1:1523348/1‑93 (MQ=255)
                                            aTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCt                                             <  2:2979787/93‑1 (MQ=255)
                                             tGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCtt                                            <  1:3208306/93‑1 (MQ=255)
                                             tGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCtt                                            <  1:4017483/93‑1 (MQ=255)
                                               gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTc                                          <  1:473477/93‑1 (MQ=255)
                                                  gagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCttt                                       >  1:3430862/1‑92 (MQ=255)
                                                  gagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCCTGCGGTGCCTTTCttt                                       >  1:233791/1‑92 (MQ=255)
                                                     gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAAGGAACCTT‑CGGGGCCCTTTTTTTCa                                    >  2:1670644/1‑93 (MQ=255)
                                                     gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                    <  1:2024902/93‑1 (MQ=255)
                                                     gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                    <  1:1478830/93‑1 (MQ=255)
                                                      tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCAt                                   <  2:2468879/93‑1 (MQ=255)
                                                        tGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTc                                 <  1:373481/93‑1 (MQ=255)
                                                         gATTCCGCTTCAACTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCCTTCa                                <  2:1750670/93‑1 (MQ=255)
                                                          aTTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCCTGCGGTGCCTTTCTTATCAtttca                               >  1:4124887/1‑90 (MQ=255)
                                                           ttACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAat                              <  2:765929/93‑1 (MQ=255)
                                                            tACGCTTCATCTTCCGCGTAATTTCTTTGCCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAata                             <  1:2587355/93‑1 (MQ=255)
                                                            tACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAata                             <  2:3600609/93‑1 (MQ=255)
                                                            tACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAata                             <  1:2774613/93‑1 (MQ=255)
                                                            tACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAata                             <  2:127956/93‑1 (MQ=255)
                                                             aCGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGACGTGGCTTTCTTATTCTTCAatat                            >  2:3368589/1‑93 (MQ=255)
                                                             aCGCTTCAGCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAatat                            >  2:4586365/1‑93 (MQ=255)
                                                               gCTTCATCTTCCGCGTAATTTCTTTACCCTGTAGGCCAACAGGTAAAGCAG‑AAAAAAGGCGCCATTCGGTGGCTTTTTTATACTTCTATATcc                          >  1:4042323/1‑93 (MQ=255)
                                                                cTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                         >  1:607486/1‑93 (MQ=255)
                                                                cTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                         <  1:1935363/93‑1 (MQ=255)
                                                                cTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                         <  1:1293904/93‑1 (MQ=255)
                                                                 ttCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCCTGCGGTGGCTTTTTTTTTATTCAATATctca                        >  1:906954/1‑90 (MQ=255)
                                                                  tCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGc                       <  1:3712498/93‑1 (MQ=255)
                                                                  tCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGc                       >  2:3558943/1‑93 (MQ=255)
                                                                    aTCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTTAATATCCAGCgg                     >  2:2804775/1‑93 (MQ=255)
                                                                    aTCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                     >  2:313048/1‑93 (MQ=255)
                                                                      cTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAAGCAACCTGCGGGGGCCTTCTTTTTATTCAATATTCAACGGAt                   >  1:3237205/1‑93 (MQ=255)
                                                                       ttCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtc                  <  1:2240477/93‑1 (MQ=255)
                                                                        tCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtct                 <  2:1417851/93‑1 (MQ=255)
                                                                         ccGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtctt                <  2:3856692/93‑1 (MQ=255)
                                                                          cgcgTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttc               <  1:4464338/93‑1 (MQ=255)
                                                                          cgcgTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttc               <  1:837647/93‑1 (MQ=255)
                                                                           gcgTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttct              >  1:3859004/1‑93 (MQ=255)
                                                                           gcgTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttct              <  2:3296360/93‑1 (MQ=255)
                                                                           gcgTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAAGCGACACGTGGGGGCTTTCTTATTATTCACTATTCATCTGCtcttct              >  2:185276/1‑93 (MQ=255)
                                                                            cgTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑CAAAAAGGCACCTTGCGGTGGCTTTTTTATTATTCAATATCCAGCAGAtcctctt             >  2:324268/1‑92 (MQ=255)
                                                                              tAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaa           >  2:4528572/1‑93 (MQ=255)
                                                                              tAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaa           <  2:898794/93‑1 (MQ=255)
                                                                               aaTTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa          >  1:304819/1‑93 (MQ=255)
                                                                               aaTTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa          <  1:3965233/93‑1 (MQ=255)
                                                                               aaTTTCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCAACTTGCCGGGCCTTTCTTATCATTCCATATCCAGGGGATATTCTGaaa          >  1:1867133/1‑93 (MQ=255)
                                                                                  ttCTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa       <  1:505556/93‑1 (MQ=255)
                                                                                    cTTTACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata     <  1:3505098/93‑1 (MQ=255)
                                                                                     tttACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa    <  2:3414107/93‑1 (MQ=255)
                                                                                     tttACCGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATAACCAGCGGATCTTCTGAAAGaataa    >  2:1137572/1‑93 (MQ=255)
                                                                                       tACCGTGTAGGCCAACAGATAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATc  <  2:2700624/93‑1 (MQ=255)
                                                                                          |                                                                                          
AGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAG‑AAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATC  >  NC_000913/4116282‑4116461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  >  NC_000913/4116279‑4116465
                                                                                             |                                                                                             
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACC                                                                                               >  SRR4302535.3120944/1‑94 (MQ=60)
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACC                                                                                               >  SRR4302535.4404933/1‑94 (MQ=60)
 ACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCG                                                                                              <  SRR4302535.4358669/94‑1 (MQ=60)
   AGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTG                                                                                            >  SRR4302535.3816511/1‑94 (MQ=60)
     CAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTA                                                                                          >  SRR4302535.2183311/1‑94 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302535.189715/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         >  SRR4302535.892643/1‑94 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         >  SRR4302535.4612362/1‑94 (MQ=60)
       GAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGG                                                                                        <  SRR4302535.598456/94‑1 (MQ=60)
       GAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGG                                                                                        <  SRR4302535.2727599/94‑1 (MQ=60)
        AACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGC                                                                                       <  SRR4302535.4451398/94‑1 (MQ=60)
        AACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGC                                                                                       <  SRR4302535.2987739/94‑1 (MQ=60)
          CGGCTGGCAGGAACGTCTGCAGTCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCA                                                                                     <  SRR4302535.917569/94‑1 (MQ=60)
          CGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCA                                                                                     >  SRR4302535.1230218/1‑94 (MQ=60)
           GGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAA                                                                                    >  SRR4302535.2914806/1‑94 (MQ=60)
               GGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGG                                                                                <  SRR4302535.439563/94‑1 (MQ=60)
               GGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGG                                                                                <  SRR4302535.2567972/94‑1 (MQ=60)
               GGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGG                                                                                <  SRR4302535.4291381/94‑1 (MQ=60)
               GGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGG                                                                                <  SRR4302535.3760154/94‑1 (MQ=60)
                GCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGT                                                                               <  SRR4302535.1636561/94‑1 (MQ=60)
                GCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGT                                                                               <  SRR4302535.260333/94‑1 (MQ=60)
                 CAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTA                                                                              <  SRR4302535.959943/94‑1 (MQ=60)
                 CAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTA                                                                              <  SRR4302535.3790649/94‑1 (MQ=60)
                 CAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTA                                                                              >  SRR4302535.4060488/1‑94 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTGCCGTGTAGGCCAACAGGTAA                                                                             <  SRR4302535.3026245/94‑1 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAA                                                                             >  SRR4302535.61809/1‑94 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAA                                                                             >  SRR4302535.1639991/1‑94 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAA                                                                             <  SRR4302535.4470665/94‑1 (MQ=60)
                   GGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAAC                                                                            <  SRR4302535.1823319/94‑1 (MQ=60)
                   GGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAAC                                                                            <  SRR4302535.1141012/94‑1 (MQ=60)
                   GGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAAC                                                                            <  SRR4302535.288032/94‑1 (MQ=60)
                    GAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACG                                                                           <  SRR4302535.2927253/94‑1 (MQ=60)
                       CGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG                                                                        <  SRR4302535.3928179/94‑1 (MQ=60)
                          CTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAA                                                                     <  SRR4302535.2845962/94‑1 (MQ=60)
                           TGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAA                                                                    <  SRR4302535.1404719/94‑1 (MQ=60)
                           TGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAA                                                                    <  SRR4302535.1367254/94‑1 (MQ=60)
                            GCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAA                                                                   <  SRR4302535.324361/94‑1 (MQ=60)
                             CAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAA                                                                  >  SRR4302535.2541736/1‑94 (MQ=60)
                              AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAG                                                                 >  SRR4302535.415313/1‑94 (MQ=60)
                              AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAG                                                                 <  SRR4302535.2413382/94‑1 (MQ=60)
                                 CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCA                                                              <  SRR4302535.4066485/94‑1 (MQ=60)
                                 CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCA                                                              <  SRR4302535.892199/94‑1 (MQ=60)
                                 CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGACA                                                              >  SRR4302535.1689554/1‑94 (MQ=60)
                                 CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTATAGGCCAACAGGTAACGCAGAAAAAAacga                                                              >  SRR4302535.824838/1‑90 (MQ=60)
                                    TGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCT                                                           >  SRR4302535.3453605/1‑94 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302535.3946899/94‑1 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302535.4166240/94‑1 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302535.2346053/94‑1 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302535.937698/94‑1 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302535.176962/94‑1 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302535.1905635/94‑1 (MQ=60)
                                      CTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTG                                                         <  SRR4302535.370494/94‑1 (MQ=60)
                                      CTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTG                                                         <  SRR4302535.2320027/94‑1 (MQ=60)
                                      CTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTG                                                         >  SRR4302535.1589453/1‑94 (MQ=60)
                                       TGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGC                                                        <  SRR4302535.2084779/94‑1 (MQ=60)
                                        GGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCG                                                       <  SRR4302535.2437076/94‑1 (MQ=60)
                                        GGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCG                                                       <  SRR4302535.2773495/94‑1 (MQ=60)
                                        GGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCG                                                       <  SRR4302535.700865/94‑1 (MQ=60)
                                        AGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCG                                                       <  SRR4302535.417241/94‑1 (MQ=60)
                                         GGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCCG                                                      >  SRR4302535.123241/1‑94 (MQ=60)
                                           TCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTG                                                    <  SRR4302535.194805/94‑1 (MQ=60)
                                            CGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAAGCACCTTGCGGTGC                                                   >  SRR4302535.3998694/1‑94 (MQ=60)
                                             GCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCC                                                  <  SRR4302535.1314382/94‑1 (MQ=60)
                                             GCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCC                                                  >  SRR4302535.1534320/1‑94 (MQ=60)
                                              CATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCT                                                 <  SRR4302535.1096125/94‑1 (MQ=60)
                                                TGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTT                                               <  SRR4302535.3230528/94‑1 (MQ=60)
                                                TGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTT                                               <  SRR4302535.4044963/94‑1 (MQ=60)
                                                 GGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTC                                              <  SRR4302535.47907/94‑1 (MQ=60)
                                                 GGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTC                                              >  SRR4302535.4594226/1‑94 (MQ=60)
                                                  GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGGCTTTCT                                             <  SRR4302535.1571919/94‑1 (MQ=60)
                                                  GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCT                                             <  SRR4302535.476791/94‑1 (MQ=60)
                                                   AAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTT                                            <  SRR4302535.113650/94‑1 (MQ=60)
                                                    AGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTT                                           >  SRR4302535.3454707/1‑94 (MQ=60)
                                                    AGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGTGCCTTTCTTT                                           >  SRR4302535.235404/1‑94 (MQ=60)
                                                     GAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAT                                          <  SRR4302535.2618316/94‑1 (MQ=60)
                                                     GAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAT                                          <  SRR4302535.129547/94‑1 (MQ=60)
                                                      AGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATC                                         <  SRR4302535.1369640/94‑1 (MQ=60)
                                                      AGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATC                                         <  SRR4302535.4612527/94‑1 (MQ=60)
                                                       GGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGAAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCA                                        >  SRR4302535.365521/1‑94 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGGACCTTGCGGTGCCcttttttttat                                       >  SRR4302535.2548293/1‑83 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAT                                       >  SRR4302535.3917717/1‑94 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAT                                       >  SRR4302535.3187495/1‑94 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAT                                       <  SRR4302535.1439611/94‑1 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAT                                       <  SRR4302535.1489413/94‑1 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAT                                       <  SRR4302535.2039494/94‑1 (MQ=60)
                                                         TCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATT                                      <  SRR4302535.1921275/94‑1 (MQ=60)
                                                           TGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCA                                    <  SRR4302535.376065/94‑1 (MQ=60)
                                                           TGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCA                                    <  SRR4302535.3817157/94‑1 (MQ=60)
                                                            GATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGTGCCTTTCTTATCATTtca                                   >  SRR4302535.4153118/1‑91 (MQ=60)
                                                               TACGCTTCATCTTCCGCGTAATTTCTTTGCCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATAT                                <  SRR4302535.2605935/94‑1 (MQ=60)
                                                               TACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATAT                                <  SRR4302535.2794366/94‑1 (MQ=60)
                                                                 CGCTTCATCTTCCGCGTAATTTCTTTACCCTGTAGGCCAACAGGTAAAGCAGAAAAAAGGCgccattcggtggcttttttatacttctatatcc                              >  SRR4302535.4069997/1‑61 (MQ=60)
                                                                  GCTTCATTTTCCGCGTAATTTCTTTGCCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             <  SRR4302535.3048951/94‑1 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             >  SRR4302535.3785613/1‑94 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             >  SRR4302535.611726/1‑94 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             >  SRR4302535.3590891/1‑94 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302535.1543006/94‑1 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302535.1949302/94‑1 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302535.1504356/94‑1 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302535.1303125/94‑1 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302535.2464673/94‑1 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGTGGCTTTTTTTTTATTCAATATCtca                            >  SRR4302535.913393/1‑91 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGAAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302535.3507711/94‑1 (MQ=60)
                                                                    TTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGC                           <  SRR4302535.2349395/94‑1 (MQ=60)
                                                                    TTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGC                           >  SRR4302535.963413/1‑94 (MQ=60)
                                                                     TCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCG                          <  SRR4302535.3738216/94‑1 (MQ=60)
                                                                      CATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                         <  SRR4302535.2078585/94‑1 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAaagaccctgccgtgtccttctttttatttaatataccacggg                        >  SRR4302535.1643568/1‑52 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        <  SRR4302535.9049/94‑1 (MQ=60)
                                                                        TCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAagcaacctgcgggggccttctttttattcaatattcaacggat                       >  SRR4302535.3259679/1‑51 (MQ=60)
                                                                        TCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAT                       <  SRR4302535.3383963/94‑1 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      >  SRR4302535.1871866/1‑94 (MQ=60)
                                                                          TTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCT                     <  SRR4302535.2256413/94‑1 (MQ=60)
                                                                             CGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCT                  <  SRR4302535.4494959/94‑1 (MQ=60)
                                                                             CGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCT                  <  SRR4302535.4500701/94‑1 (MQ=60)
                                                                             CGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCT                  >  SRR4302535.1420905/1‑94 (MQ=60)
                                                                             CGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCT                  >  SRR4302535.3885563/1‑94 (MQ=60)
                                                                             CGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCT                  <  SRR4302535.843527/94‑1 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA               >  SRR4302535.2348448/1‑94 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA               <  SRR4302535.841555/94‑1 (MQ=60)
                                                                                 TAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAA              >  SRR4302535.306934/1‑94 (MQ=60)
                                                                                 TAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAACTTGCCGGGCCTTTCTTATCATTCCATATCCAGGGGATATTCTGAAA              >  SRR4302535.1880588/1‑94 (MQ=60)
                                                                                  AATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAG             <  SRR4302535.3992321/94‑1 (MQ=60)
                                                                                   ATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGA            <  SRR4302535.514838/94‑1 (MQ=60)
                                                                                   ATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGA            <  SRR4302535.1165050/94‑1 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          <  SRR4302535.509089/94‑1 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          >  SRR4302535.2183456/1‑94 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          <  SRR4302535.2019212/94‑1 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        <  SRR4302535.3529456/94‑1 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        <  SRR4302535.3735891/94‑1 (MQ=60)
                                                                                          TACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCC     <  SRR4302535.3964458/94‑1 (MQ=60)
                                                                                          TACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGGGCCTTTCTTATCATTCAATATCCAACGGATCTTCTGAAAGAATAATCC     >  SRR4302535.4033888/1‑94 (MQ=60)
                                                                                           ACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCC    <  SRR4302535.2657531/94‑1 (MQ=60)
                                                                                           ACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCC    >  SRR4302535.3934452/1‑94 (MQ=60)
                                                                                             CGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  <  SRR4302535.3816325/94‑1 (MQ=60)
                                                                                             |                                                                                             
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  >  NC_000913/4116279‑4116465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑