Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,176,909 A→G E151E (GAA→GAG)  rplA → 50S ribosomal subunit protein L1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,176,9090AG100.0% 745.7 / NA 235E151E (GAA→GAG) rplA50S ribosomal subunit protein L1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (99/136);  total (99/136)

Only 100 of 240 total aligned reads displayed.

GCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGA  >  NC_000913/4176819‑4176998
                                                                                          |                                                                                         
gcgcGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGc                                                                                         <  1:1067745/93‑1 (MQ=255)
    gttgttGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGtt                                                                                     <  2:512512/93‑1 (MQ=255)
    gttgttGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGtt                                                                                     >  2:4033699/1‑93 (MQ=255)
       gttgGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGCACTGTAACAACGAACGTTGCTGAGGCGGTTaaa                                                                                  >  2:4478878/1‑93 (MQ=255)
         tgGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTaaaaa                                                                                <  1:1190525/93‑1 (MQ=255)
         tgGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTaaaaa                                                                                <  2:4096540/93‑1 (MQ=255)
         tgGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTaaaaa                                                                                <  1:4220831/93‑1 (MQ=255)
          ggCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAAc                                                                               <  2:4451922/93‑1 (MQ=255)
            ccAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGc                                                                             <  1:3426064/93‑1 (MQ=255)
             cAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCt                                                                            >  1:3680578/1‑93 (MQ=255)
             cAGCTGGGCCAGGTTCTGGGTCAGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCt                                                                            >  2:3105282/1‑93 (MQ=255)
              aGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTa                                                                           <  1:3445450/93‑1 (MQ=255)
               gCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTaa                                                                          >  1:3601773/1‑93 (MQ=255)
               gCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTaa                                                                          >  1:3911973/1‑93 (MQ=255)
                cTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTaaa                                                                         <  2:1668180/93‑1 (MQ=255)
                cTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTaaa                                                                         <  1:4026615/93‑1 (MQ=255)
                 tGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAg                                                                        <  1:307595/93‑1 (MQ=255)
                   ggCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCt                                                                      >  2:1432950/1‑93 (MQ=255)
                   gaccAGGTTCTGGGTCCGCGCGGCCTGATGACAAGCCCGAAAGTGGCTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCt                                                                      <  2:1737515/91‑1 (MQ=255)
                         gTTCTGGGTCCGCGCGGCCTGCTGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAg                                                                >  1:3869054/1‑93 (MQ=255)
                         gTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAg                                                                <  2:3048378/93‑1 (MQ=255)
                         gTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAg                                                                <  1:1923788/93‑1 (MQ=255)
                         gTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAg                                                                <  1:1963913/93‑1 (MQ=255)
                           tCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGt                                                              <  2:3940506/93‑1 (MQ=255)
                           tCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGt                                                              <  2:2690579/93‑1 (MQ=255)
                            cTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGtt                                                             <  1:2814555/93‑1 (MQ=255)
                              gggTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCg                                                           >  1:2753768/1‑93 (MQ=255)
                              gggTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCg                                                           <  1:2188097/93‑1 (MQ=255)
                              gggTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGAAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCg                                                           <  2:3395989/93‑1 (MQ=255)
                               ggTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGt                                                          >  1:81033/1‑93 (MQ=255)
                               ggTCCGCGCGGCCGGATGCCATACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGt                                                          <  2:806371/93‑1 (MQ=255)
                                gTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGtt                                                         <  1:917027/93‑1 (MQ=255)
                                 tCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTa                                                        >  1:3092823/1‑93 (MQ=255)
                                 tCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTa                                                        >  1:2948966/1‑93 (MQ=255)
                                 tCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTa                                                        <  1:546546/93‑1 (MQ=255)
                                 tCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTa                                                        >  1:1878585/1‑93 (MQ=255)
                                  ccGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTAc                                                       <  2:3838703/93‑1 (MQ=255)
                                  ccGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTAc                                                       <  1:4438897/93‑1 (MQ=255)
                                    gcgcgGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCg                                                     >  2:1825690/1‑93 (MQ=255)
                                    gcgcgGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCg                                                     >  1:304536/1‑93 (MQ=255)
                                    gcgcgGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCg                                                     <  1:1675152/93‑1 (MQ=255)
                                      gcgGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTa                                                   >  2:1579701/1‑93 (MQ=255)
                                      gcgGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTa                                                   >  1:943277/1‑93 (MQ=255)
                                        ggCCTGATGCCAAACCCGAAAGTGTGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAAc                                                 <  1:704677/93‑1 (MQ=255)
                                        ggCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAAc                                                 <  1:153487/93‑1 (MQ=255)
                                         gCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACg                                                >  1:4070091/1‑93 (MQ=255)
                                         gCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACg                                                >  1:2401792/1‑93 (MQ=255)
                                         gCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACg                                                >  2:1446801/1‑93 (MQ=255)
                                          ccTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGa                                               <  1:242566/93‑1 (MQ=255)
                                           cTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGTTTCGTTACCGTAACGAc                                              <  1:1343523/93‑1 (MQ=255)
                                           cTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGAc                                              <  2:295258/93‑1 (MQ=255)
                                             gATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACaa                                            <  2:2795486/93‑1 (MQ=255)
                                             gATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACaa                                            <  1:133203/93‑1 (MQ=255)
                                                gCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACaaaaa                                         <  2:630018/93‑1 (MQ=255)
                                                gCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACaaaaa                                         <  2:2507344/93‑1 (MQ=255)
                                                 ccAATCCCGAAGGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAACCGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAAc                                        <  1:25533/93‑1 (MQ=255)
                                                  cAATCCCGAAAGTGGGTACTTTAACTCCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACg                                       <  1:2298979/93‑1 (MQ=255)
                                                   aaaCCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACgg                                      <  2:3919976/93‑1 (MQ=255)
                                                    aaCCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGc                                     >  1:4597120/1‑93 (MQ=255)
                                                        cGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGcatca                                 >  2:736076/1‑93 (MQ=255)
                                                        cGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGcatca                                 <  1:1111882/93‑1 (MQ=255)
                                                         gAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGcatcat                                >  1:4361996/1‑93 (MQ=255)
                                                          aaaGTGGGTACTGTAACACCGACCGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCatcatc                               <  2:2419153/93‑1 (MQ=255)
                                                           aaGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATcc                              <  2:1181876/93‑1 (MQ=255)
                                                           aaGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATcc                              <  2:2103818/93‑1 (MQ=255)
                                                            aGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCca                             >  2:2402819/1‑93 (MQ=255)
                                                              tGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCcaca                           <  2:3231135/93‑1 (MQ=255)
                                                                ggTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCAcacc                         >  2:3971717/1‑93 (MQ=255)
                                                                 gTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCAcacca                        >  1:1449986/1‑93 (MQ=255)
                                                                 gTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCAcacca                        >  1:3276939/1‑93 (MQ=255)
                                                                  tACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCAcaccac                       <  1:455569/93‑1 (MQ=255)
                                                                     tGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCAt                    >  1:1859126/1‑93 (MQ=255)
                                                                      gTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATc                   >  2:4422574/1‑93 (MQ=255)
                                                                       tAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCg                  >  2:342013/1‑93 (MQ=255)
                                                                       tAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCg                  >  1:206015/1‑93 (MQ=255)
                                                                        aaCACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCgg                 >  2:2564154/1‑93 (MQ=255)
                                                                            ccGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTaaa             <  1:760633/93‑1 (MQ=255)
                                                                             cGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAg            >  2:1906910/1‑93 (MQ=255)
                                                                              gagcGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGt           <  2:4282429/90‑1 (MQ=255)
                                                                              gAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGt           >  2:717999/1‑93 (MQ=255)
                                                                              gAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGt           <  2:2157406/93‑1 (MQ=255)
                                                                               aaCGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTg          <  2:1116573/93‑1 (MQ=255)
                                                                               aaCGTTGCTGAAGCGGTTAAAAACGCTAAAGGTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACCCCACCATCGGTAAAGTg          <  1:32049/93‑1 (MQ=255)
                                                                                aCGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTgg         >  1:3560469/1‑93 (MQ=255)
                                                                                aCGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTgg         >  2:4191991/1‑93 (MQ=255)
                                                                                  gTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTCCCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGAc       <  1:188902/93‑1 (MQ=255)
                                                                                  gTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGAc       >  2:3551091/1‑93 (MQ=255)
                                                                                  gTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGAc       <  1:2869663/93‑1 (MQ=255)
                                                                                  gTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGAc       >  1:577210/1‑93 (MQ=255)
                                                                                  gTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGAc       <  1:1003516/93‑1 (MQ=255)
                                                                                    tGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCTTTACCGTACCGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACtt     <  2:3625127/93‑1 (MQ=255)
                                                                                     gCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACttt    >  1:1232723/1‑93 (MQ=255)
                                                                                     gCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACttt    <  1:4647312/93‑1 (MQ=255)
                                                                                     gCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACttt    >  2:2989032/1‑93 (MQ=255)
                                                                                     gCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACttt    <  2:777692/93‑1 (MQ=255)
                                                                                      cTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTg   <  2:4346115/93‑1 (MQ=255)
                                                                                       tGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGa  <  2:193772/93‑1 (MQ=255)
                                                                                       tGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGa  <  2:3794791/93‑1 (MQ=255)
                                                                                       tGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGa  >  1:3187846/1‑93 (MQ=255)
                                                                                       tGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGa  <  2:928738/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGA  >  NC_000913/4176819‑4176998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGCGCGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCT  >  NC_000913/4176816‑4177002
                                                                                              |                                                                                             
AATGCGTGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAG                                                                                               <  SRR4302535.909991/94‑1 (MQ=60)
AATGCGCGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAG                                                                                               >  SRR4302535.3820949/1‑94 (MQ=60)
AATGCGCGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAG                                                                                               >  SRR4302535.4558213/1‑94 (MQ=60)
   GCGCGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCG                                                                                            <  SRR4302535.1075340/94‑1 (MQ=60)
   GCGCGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCG                                                                                            <  SRR4302535.1966506/94‑1 (MQ=60)
   GCGCGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCG                                                                                            <  SRR4302535.4426487/94‑1 (MQ=60)
      CGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTT                                                                                         <  SRR4302535.2442396/94‑1 (MQ=60)
          GTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAA                                                                                     >  SRR4302535.3926869/1‑94 (MQ=60)
           TTGGCCAGCTGGGC‑CAGGTTCT‑GGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAAC                                                                                   <  SRR4302535.991855/94‑1 (MQ=60)
            TGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAAC                                                                                   <  SRR4302535.288579/94‑1 (MQ=60)
            TGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAAC                                                                                   <  SRR4302535.1199049/94‑1 (MQ=60)
            TGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAAC                                                                                   <  SRR4302535.4249713/94‑1 (MQ=60)
             GGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACG                                                                                  <  SRR4302535.3403849/94‑1 (MQ=60)
               CCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCT                                                                                >  SRR4302535.3706051/1‑94 (MQ=60)
               CCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCT                                                                                <  SRR4302535.3449888/94‑1 (MQ=60)
                CAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTA                                                                               >  SRR4302535.679788/1‑94 (MQ=60)
                 AGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAA                                                                              >  SRR4302535.3626768/1‑94 (MQ=60)
                 AGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAA                                                                              >  SRR4302535.3686562/1‑94 (MQ=60)
                 AGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAA                                                                              <  SRR4302535.3469406/94‑1 (MQ=60)
                 AGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAA                                                                              >  SRR4302535.3938753/1‑94 (MQ=60)
                  GCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAA                                                                             <  SRR4302535.2368533/94‑1 (MQ=60)
                   CTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAG                                                                            <  SRR4302535.247592/94‑1 (MQ=60)
                   CTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAG                                                                            <  SRR4302535.4054164/94‑1 (MQ=60)
                    TGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGC                                                                           <  SRR4302535.309724/94‑1 (MQ=60)
                     GGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCT                                                                          <  SRR4302535.3998708/94‑1 (MQ=60)
                      GGCGCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCT                                                                          <  SRR4302535.221566/94‑1 (MQ=60)
                      GGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTG                                                                         <  SRR4302535.3083117/94‑1 (MQ=60)
                       GC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGG                                                                        >  SRR4302535.3605650/1‑94 (MQ=60)
                          CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCC                                                                      >  SRR4302535.706009/1‑94 (MQ=60)
                           AGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCA                                                                     >  SRR4302535.796417/1‑94 (MQ=60)
                           AGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCA                                                                     >  SRR4302535.715582/1‑94 (MQ=60)
                            GGTTCTGGGTCCGCGCGGCCTGCTGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAG                                                                    >  SRR4302535.3895649/1‑94 (MQ=60)
                             GTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGG                                                                   <  SRR4302535.1937652/94‑1 (MQ=60)
                             GTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGG                                                                   <  SRR4302535.1978072/94‑1 (MQ=60)
                             GTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGG                                                                   <  SRR4302535.899068/94‑1 (MQ=60)
                             GTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGG                                                                   >  SRR4302535.1349493/1‑94 (MQ=60)
                              TTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGT                                                                  <  SRR4302535.136968/94‑1 (MQ=60)
                               TCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTT                                                                 <  SRR4302535.928431/94‑1 (MQ=60)
                               TCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTT                                                                 <  SRR4302535.1712353/94‑1 (MQ=60)
                                CTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTC                                                                <  SRR4302535.2834554/94‑1 (MQ=60)
                                 TGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCG                                                               >  SRR4302535.2773393/1‑94 (MQ=60)
                                  GGGTCGGCGCGGCCTGATGCCAAACCCGAGAGCGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAACTGGCCAcgctggt                                                              >  SRR4302535.115846/1‑87 (MQ=60)
                                  GGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGT                                                              <  SRR4302535.2203682/94‑1 (MQ=60)
                                  GGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGT                                                              >  SRR4302535.81611/1‑94 (MQ=60)
                                    GTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTA                                                            >  SRR4302535.3114360/1‑94 (MQ=60)
                                    GTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTA                                                            <  SRR4302535.923534/94‑1 (MQ=60)
                                    GTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTA                                                            >  SRR4302535.2969684/1‑94 (MQ=60)
                                    GTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTA                                                            >  SRR4302535.1892122/1‑94 (MQ=60)
                                    GTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTA                                                            <  SRR4302535.1934721/94‑1 (MQ=60)
                                     TCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTAC                                                           <  SRR4302535.550367/94‑1 (MQ=60)
                                      CCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACC                                                          <  SRR4302535.1024843/94‑1 (MQ=60)
                                      CCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACC                                                          <  SRR4302535.2390974/94‑1 (MQ=60)
                                      CCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACC                                                          <  SRR4302535.4469324/94‑1 (MQ=60)
                                       CGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCG                                                         >  SRR4302535.306649/1‑94 (MQ=60)
                                       CGCGCGGCCTGATG‑CAAAACCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGT                                                        <  SRR4302535.4489887/94‑1 (MQ=60)
                                        GCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGT                                                        <  SRR4302535.1687280/94‑1 (MQ=60)
                                         CGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTA                                                       >  SRR4302535.949952/1‑94 (MQ=60)
                                            GGCCTGATGCCAAACCCGAAAGTGTGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACG                                                    <  SRR4302535.709626/94‑1 (MQ=60)
                                            GGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACG                                                    <  SRR4302535.154565/94‑1 (MQ=60)
                                            GGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACG                                                    >  SRR4302535.2050700/1‑94 (MQ=60)
                                            GGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACG                                                    >  SRR4302535.1263161/1‑94 (MQ=60)
                                            GGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACG                                                    <  SRR4302535.3008174/94‑1 (MQ=60)
                                            GGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACG                                                    >  SRR4302535.4097944/1‑94 (MQ=60)
                                            GGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACG                                                    <  SRR4302535.1856364/94‑1 (MQ=60)
                                            GGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACG                                                    >  SRR4302535.2419068/1‑94 (MQ=60)
                                             GCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGA                                                   >  SRR4302535.1920783/1‑94 (MQ=60)
                                              CCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGAC                                                  <  SRR4302535.244228/94‑1 (MQ=60)
                                              CCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGAC                                                  >  SRR4302535.2872486/1‑94 (MQ=60)
                                               CTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGTTTCGTTACCGTAACGACA                                                 <  SRR4302535.1353113/94‑1 (MQ=60)
                                               CTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACA                                                 <  SRR4302535.3708119/94‑1 (MQ=60)
                                                 GATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAA                                               <  SRR4302535.134136/94‑1 (MQ=60)
                                                    TCCAAACCCGAAAGGGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAAC                                            <  SRR4302535.1154097/94‑1 (MQ=60)
                                                    GCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAAC                                            <  SRR4302535.329387/94‑1 (MQ=60)
                                                    GCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAAC                                            <  SRR4302535.4066736/94‑1 (MQ=60)
                                                     CCAATCCCGAAGGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAACCGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACG                                           <  SRR4302535.25730/94‑1 (MQ=60)
                                                     CCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACG                                           <  SRR4302535.1897926/94‑1 (MQ=60)
                                                     CCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACG                                           <  SRR4302535.3221017/94‑1 (MQ=60)
                                                     CCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACG                                           >  SRR4302535.3280199/1‑94 (MQ=60)
                                                     CCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACG                                           <  SRR4302535.2926395/94‑1 (MQ=60)
                                                     CCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACG                                           <  SRR4302535.1086642/94‑1 (MQ=60)
                                                     CCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACG                                           <  SRR4302535.3507004/94‑1 (MQ=60)
                                                      CAATCCCGAAAGTGGGTACTTTAACTCCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGG                                          <  SRR4302535.2315426/94‑1 (MQ=60)
                                                       AAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGC                                         >  SRR4302535.3568447/1‑94 (MQ=60)
                                                       AAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGC                                         >  SRR4302535.4628395/1‑94 (MQ=60)
                                                       AAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGC                                         >  SRR4302535.4677555/1‑94 (MQ=60)
                                                        AACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCA                                        >  SRR4302535.4674380/1‑94 (MQ=60)
                                                         ACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCAT                                       <  SRR4302535.1482878/94‑1 (MQ=60)
                                                           CCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCA                                     <  SRR4302535.368642/94‑1 (MQ=60)
                                                           CCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCA                                     >  SRR4302535.4143225/1‑94 (MQ=60)
                                                            CGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCAT                                    <  SRR4302535.1119816/94‑1 (MQ=60)
                                                            CGAAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCAT                                    >  SRR4302535.4391847/1‑94 (MQ=60)
                                                              AAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCC                                  <  SRR4302535.4143259/94‑1 (MQ=60)
                                                              AAAGTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCC                                  >  SRR4302535.2540704/1‑94 (MQ=60)
                                                                 GTGGGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACA                               >  SRR4302535.3100828/1‑94 (MQ=60)
                                                                    GGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCC                            >  SRR4302535.2711238/1‑94 (MQ=60)
                                                                    GGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCA                            >  SRR4302535.3299702/1‑94 (MQ=60)
                                                                    GGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCA                            >  SRR4302535.1460356/1‑94 (MQ=60)
                                                                    GGTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCA                            >  SRR4302535.771553/1‑94 (MQ=60)
                                                                     GTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCAC                           >  SRR4302535.3118058/1‑94 (MQ=60)
                                                                     GTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCAC                           >  SRR4302535.1248919/1‑94 (MQ=60)
                                                                     GTACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCAC                           >  SRR4302535.2125741/1‑94 (MQ=60)
                                                                      TACTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACC                          <  SRR4302535.458756/94‑1 (MQ=60)
                                                                        CTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCAT                        >  SRR4302535.1872531/1‑94 (MQ=60)
                                                                        CTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCAT                        >  SRR4302535.1686511/1‑94 (MQ=60)
                                                                        CTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCAT                        <  SRR4302535.4562544/94‑1 (MQ=60)
                                                                        CTGTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCAT                        >  SRR4302535.313026/1‑94 (MQ=60)
                                                                          GTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCG                      <  SRR4302535.2781615/94‑1 (MQ=60)
                                                                          GTAACACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCG                      >  SRR4302535.207430/1‑94 (MQ=60)
                                                                              CACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAA                  <  SRR4302535.568383/94‑1 (MQ=60)
                                                                              CACCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAA                  >  SRR4302535.484511/1‑94 (MQ=60)
                                                                                CCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAG                <  SRR4302535.765992/94‑1 (MQ=60)
                                                                                CCGAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAG                >  SRR4302535.153833/1‑94 (MQ=60)
                                                                                CCGAACGTTGCTGAGGCGGTTAAAAACCCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAG                <  SRR4302535.4041672/94‑1 (MQ=60)
                                                                                  GAACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTG              <  SRR4302535.1891102/94‑1 (MQ=60)
                                                                                   AACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGG             <  SRR4302535.3048886/94‑1 (MQ=60)
                                                                                   AACGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGG             >  SRR4302535.3585157/1‑94 (MQ=60)
                                                                                   AACGTTGCTGAAGCGGTTAAAAACGCTAAAGGTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACCCCACCATCGGTAAAGTGG             <  SRR4302535.32295/94‑1 (MQ=60)
                                                                                     CGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGAC           >  SRR4302535.581265/1‑94 (MQ=60)
                                                                                     CGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGAC           >  SRR4302535.4345441/1‑94 (MQ=60)
                                                                                     CGTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGAC           >  SRR4302535.1209611/1‑94 (MQ=60)
                                                                                      GTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTCCCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACT          <  SRR4302535.190207/94‑1 (MQ=60)
                                                                                      GTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACT          <  SRR4302535.1010640/94‑1 (MQ=60)
                                                                                      GTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACT          >  SRR4302535.1159892/1‑94 (MQ=60)
                                                                                      GTTGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACT          <  SRR4302535.2889985/94‑1 (MQ=60)
                                                                                        TGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTT        <  SRR4302535.4608/94‑1 (MQ=60)
                                                                                        TGCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTT        >  SRR4302535.1241540/1‑94 (MQ=60)
                                                                                         GCTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTG       <  SRR4302535.4678794/94‑1 (MQ=60)
                                                                                          CTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTATCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGA      >  SRR4302535.2046939/1‑94 (MQ=60)
                                                                                          CTGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGA      >  SRR4302535.3209893/1‑94 (MQ=60)
                                                                                           TGAGGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGAC     <  SRR4302535.3468072/94‑1 (MQ=60)
                                                                                              GGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCT  <  SRR4302535.3683687/94‑1 (MQ=60)
                                                                                              GGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCT  <  SRR4302535.2608686/94‑1 (MQ=60)
                                                                                              |                                                                                             
AATGCGCGTTGTTGGCCAGCTGGGC‑CAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACCCGAAAGTGGGTACTGTAACACCGAACGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCT  >  NC_000913/4176816‑4177002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑