Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,227,795	A→G	intergenic (‑74/‑138)	pepE ← / → yjbC	peptidase E/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,227,795	0	A	G	99.6%	729.8 / ‑5.0	236	intergenic (‑74/‑138)	pepE/yjbC	peptidase E/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (103/130); total (105/131)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

Only 100 of 253 total aligned reads displayed.

AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTT  >  NC_000913/4227705‑4227884

aaTAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTcgcg                                                                                         >  1:2732063/1‑93 (MQ=255)

 aTAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGa                                                                                        >  2:4279611/1‑93 (MQ=255)

  tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGAt                                                                                       <  2:2566105/93‑1 (MQ=255)

  tAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGAt                                                                                       <  2:2582635/93‑1 (MQ=255)

   aaaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATg                                                                                      <  1:1621933/93‑1 (MQ=255)

    aaaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGt                                                                                     <  2:1331578/93‑1 (MQ=255)

     aaGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTc                                                                                    <  2:643961/93‑1 (MQ=255)

      aGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCg                                                                                   >  1:2909730/1‑93 (MQ=255)

         aGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGcac                                                                                >  1:1571929/1‑93 (MQ=255)

           ttCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAt                                                                              >  2:1146966/1‑93 (MQ=255)

            tCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAtt                                                                             <  1:822311/93‑1 (MQ=255)

            tCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAtt                                                                             <  2:2481230/93‑1 (MQ=255)

             ccATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTg                                                                            >  1:4609691/1‑93 (MQ=255)

              cATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGc                                                                           >  2:1826028/1‑93 (MQ=255)

               aTTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCt                                                                          >  2:895459/1‑93 (MQ=255)

               aTTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCt                                                                          >  1:404947/1‑93 (MQ=255)

                  aCTTCTCCGGTTAGTAAGAAATTACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCtctt                                                                       >  2:3774980/1‑90 (MQ=255)

                  aCTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCt                                                                       >  2:4654085/1‑93 (MQ=255)

                   cTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTg                                                                      <  1:3009687/93‑1 (MQ=255)

                   cTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTg                                                                      >  2:2983954/1‑93 (MQ=255)

                    ttCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACACTGTGTCTgg                                                                     >  1:2118093/1‑93 (MQ=255)

                     tctcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGa                                                                    >  1:2230595/1‑93 (MQ=255)

                     tctcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGa                                                                    <  2:447582/93‑1 (MQ=255)

                     tctcCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGa                                                                    >  2:1423061/1‑93 (MQ=255)

                         cGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAgg                                                                >  1:2398326/1‑92 (MQ=255)

                         cGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAgg                                                                >  1:3780961/1‑92 (MQ=255)

                         cGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGt                                                                <  1:283826/93‑1 (MQ=255)

                         cGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGt                                                                <  1:1962550/93‑1 (MQ=255)

                         cGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGt                                                                <  2:1947039/93‑1 (MQ=255)

                          ggTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTg                                                               >  2:190772/1‑93 (MQ=255)

                          ggTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTg                                                               >  1:3739587/1‑93 (MQ=255)

                            ttAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGGTGTAGCACATTGCCTCTGGAAAGTggt                                                             >  2:1870302/1‑91 (MQ=255)

                            ttAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGAt                                                             >  2:1742646/1‑93 (MQ=255)

                            ttAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAt                                                             <  1:4663599/93‑1 (MQ=255)

                             tAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAtt                                                            <  1:4651660/93‑1 (MQ=255)

                             tAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAtg                                                            >  1:4501646/1‑92 (MQ=255)

                              aGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGAttt                                                           <  2:3332254/93‑1 (MQ=255)

                               gTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGGTTCTGGGAAGAGATTTg                                                          >  2:126210/1‑93 (MQ=255)

                                tAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGa                                                         >  1:1143998/1‑93 (MQ=255)

                                   gAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCa                                                      <  1:173904/93‑1 (MQ=255)

                                   gAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCa                                                      <  1:806768/93‑1 (MQ=255)

                                    aaaTCACGGGAGACATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAg                                                     <  2:1515411/93‑1 (MQ=255)

                                      aTCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGt                                                   >  2:1775099/1‑93 (MQ=255)

                                        cACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGTaa                                                 >  2:2412138/1‑93 (MQ=255)

                                           gggAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGa                                              >  1:177689/1‑93 (MQ=255)

                                            ggAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGGAACGaa                                             >  1:1286630/1‑93 (MQ=255)

                                            ggAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGaa                                             >  1:358845/1‑93 (MQ=255)

                                                gCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCa                                         <  1:2009022/93‑1 (MQ=255)

                                                gCATGCTACCACTGCGAATTTAACCACTTACTGAAATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCa                                         <  1:2951419/93‑1 (MQ=255)

                                                 cATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCaa                                        <  2:1558650/93‑1 (MQ=255)

                                                 cATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCaa                                        <  1:1042015/93‑1 (MQ=255)

                                                 cATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCaa                                        <  1:3852540/93‑1 (MQ=255)

                                                  aTGCTACCACTGCGAATTTAACCACTTACTGACATGACTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAg                                       <  1:2716717/93‑1 (MQ=255)

                                                  aTGCTACCACTGCAAATGTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGGAGGTAACGAATTCAAg                                       <  2:4006663/93‑1 (MQ=255)

                                                   tGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCCCGGAGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGGAACGAATTCAAGc                                      >  2:2233462/1‑93 (MQ=255)

                                                    gCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGcc                                     <  1:1683650/93‑1 (MQ=255)

                                                      tACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   >  1:3917582/1‑93 (MQ=255)

                                                      tACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   <  1:3275827/93‑1 (MQ=255)

                                                      tACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCaa                                   <  2:3401690/93‑1 (MQ=255)

                                                       aCCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAAt                                  <  1:2859865/93‑1 (MQ=255)

                                                         cACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATgg                                <  2:1625306/93‑1 (MQ=255)

                                                          aCTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGa                               <  1:3648683/93‑1 (MQ=255)

                                                          aCTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGa                               <  1:352828/93‑1 (MQ=255)

                                                          aCTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGa                               <  2:2345283/93‑1 (MQ=255)

                                                          aCTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGa                               >  2:3868614/1‑93 (MQ=255)

                                                          aCTGCGAATTTAACCACTTACTGACATGGCTCGCGAGGGCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGGAACGAATTCAAGACAATGGa                               >  2:4222291/1‑93 (MQ=255)

                                                            tGCGAATTTAGCCACTTACTGACAGGCCGCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCGGGAAACGAGTTCAAGCCAATGGaaa                             <  2:2051307/93‑1 (MQ=255)

                                                            tGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGaaa                             <  2:291367/93‑1 (MQ=255)

                                                            tGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGaaa                             >  2:1067360/1‑93 (MQ=255)

                                                            tGCGAATTTAAACACATACTGACATGGCTCGCCATGTCGCACATGGCTTCTGGAAAGTGATTTGAGCAGTTAACGAATTCAAGCCAATGGaaa                             <  2:2328813/93‑1 (MQ=255)

                                                             gCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAAt                            <  1:1230897/93‑1 (MQ=255)

                                                             gCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCGGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAAt                            <  1:3093851/93‑1 (MQ=255)

                                                                aaTTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAAc                         <  2:2729145/93‑1 (MQ=255)

                                                                aaTTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAAc                         >  2:3287792/1‑93 (MQ=255)

                                                                 aTTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACa                        >  1:4339533/1‑93 (MQ=255)

                                                                  tttAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAATCCAATGGCAATAACaa                       >  1:2480479/1‑93 (MQ=255)

                                                                  tttAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACaa                       >  2:3081063/1‑93 (MQ=255)

                                                                   ttAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACaaa                      <  2:278025/93‑1 (MQ=255)

                                                                    tAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAAt                     >  1:1543241/1‑93 (MQ=255)

                                                                    tAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAAt                     <  1:1520311/93‑1 (MQ=255)

                                                                     aaCCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGGAACGAATTCAAGCCAATGGAAATAACAAATa                    >  1:2034266/1‑93 (MQ=255)

                                                                       ccccTTACTGACATGGCTCGCGATGTCCCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGt                  <  2:4172616/90‑1 (MQ=255)

                                                                       ccACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGt                  <  2:4378290/93‑1 (MQ=255)

                                                                        cACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGtt                 <  1:4207793/93‑1 (MQ=255)

                                                                        cACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGtt                 <  2:3644414/93‑1 (MQ=255)

                                                                        cACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGtt                 <  2:1270802/93‑1 (MQ=255)

                                                                         aCTTACTGACATGGCTCGCGATGTCGCGCATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTc                >  2:824679/1‑93 (MQ=255)

                                                                         aCTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGGAATAACAAATAGTTc                >  1:3165500/1‑93 (MQ=255)

                                                                          cTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCg               >  2:214973/1‑93 (MQ=255)

                                                                           ttccTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGc              <  2:1986340/90‑1 (MQ=255)

                                                                           ttACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGc              >  1:3435935/1‑93 (MQ=255)

                                                                           ttACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGc              >  2:2290190/1‑93 (MQ=255)

                                                                             aCTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTc            <  2:3433230/93‑1 (MQ=255)

                                                                               tGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAt          <  1:635780/93‑1 (MQ=255)

                                                                                gACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATg         <  1:966046/93‑1 (MQ=255)

                                                                                 aCATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGa        >  1:1008206/1‑93 (MQ=255)

                                                                                   aTGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACt      >  1:3221505/1‑93 (MQ=255)

                                                                                    tGGCTCGCGATGTCGCACATTGATTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTa     <  1:4546193/93‑1 (MQ=255)

                                                                                     ggCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAt    <  2:1859364/93‑1 (MQ=255)

                                                                                      gCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAtt   >  1:729698/1‑93 (MQ=255)

AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTT  >  NC_000913/4227705‑4227884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTTTT  >  NC_000913/4227702‑4227887

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCG                                                                                              >  SRR4302535.29144/1‑94 (MQ=60)

  CAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCG                                                                                            >  SRR4302535.2751567/1‑94 (MQ=60)

  CAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCG                                                                                            <  SRR4302535.3483408/94‑1 (MQ=60)

   AATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGA                                                                                           >  SRR4302535.2986468/1‑94 (MQ=60)

     TAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCGCTTACTGACATGGCTCGCGATG                                                                                         <  SRR4302535.3109236/94‑1 (MQ=60)

     TAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATG                                                                                         <  SRR4302535.2059969/94‑1 (MQ=60)

     TAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATG                                                                                         >  SRR4302535.1578498/1‑94 (MQ=60)

      AAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGT                                                                                        <  SRR4302535.1633641/94‑1 (MQ=60)

       AAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTC                                                                                       >  SRR4302535.637656/1‑94 (MQ=60)

        AAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCG                                                                                      >  SRR4302535.2930288/1‑94 (MQ=60)

         AGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGC                                                                                     >  SRR4302535.813671/1‑94 (MQ=60)

          GCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCA                                                                                    <  SRR4302535.3964579/94‑1 (MQ=60)

           CAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCAC                                                                                   <  SRR4302535.3551445/94‑1 (MQ=60)

           CAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCAC                                                                                   >  SRR4302535.1583276/1‑94 (MQ=60)

           CAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCAC                                                                                   <  SRR4302535.3221698/94‑1 (MQ=60)

            AGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACA                                                                                  >  SRR4302535.2568852/1‑94 (MQ=60)

            AGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACA                                                                                  >  SRR4302535.388347/1‑94 (MQ=60)

             GTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACAT                                                                                 >  SRR4302535.3753243/1‑94 (MQ=60)

              TTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATT                                                                                <  SRR4302535.4691265/94‑1 (MQ=60)

               TCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTG                                                                               >  SRR4302535.4641013/1‑94 (MQ=60)

               TCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTG                                                                               <  SRR4302535.828074/94‑1 (MQ=60)

               TCCATTACTGCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTG                                                                               <  SRR4302535.1903924/94‑1 (MQ=60)

               GCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTG                                                                               <  SRR4302535.4532974/94‑1 (MQ=60)

                CCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTTGCACATTGC                                                                              >  SRR4302535.464481/1‑94 (MQ=60)

                CCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGC                                                                              >  SRR4302535.2399724/1‑94 (MQ=60)

                 CATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCT                                                                             >  SRR4302535.407783/1‑94 (MQ=60)

                  ATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTTCTT                                                                            >  SRR4302535.2495018/1‑94 (MQ=60)

                  ATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTT                                                                            >  SRR4302535.1097014/1‑94 (MQ=60)

                   TTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTC                                                                           >  SRR4302535.3287900/1‑94 (MQ=60)

                    TACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCT                                                                          <  SRR4302535.4074072/94‑1 (MQ=60)

                    TACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCT                                                                          <  SRR4302535.3847588/94‑1 (MQ=60)

                     ACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTG                                                                         <  SRR4302535.4112588/94‑1 (MQ=60)

                      CTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACActgtgtctgg                                                                        >  SRR4302535.2133371/1‑84 (MQ=60)

                      CTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGG                                                                        <  SRR4302535.3030770/94‑1 (MQ=60)

                      CTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGG                                                                        <  SRR4302535.4321660/94‑1 (MQ=60)

                      CTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGG                                                                        <  SRR4302535.1266585/94‑1 (MQ=60)

                       TTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGA                                                                       >  SRR4302535.2246456/1‑94 (MQ=60)

                         CTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAA                                                                     >  SRR4302535.3719337/1‑94 (MQ=60)

                          TCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAG                                                                    >  SRR4302535.1745326/1‑94 (MQ=60)

                          TCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAG                                                                    <  SRR4302535.2868803/94‑1 (MQ=60)

                           CCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGG                                                                   >  SRR4302535.2415571/1‑94 (MQ=60)

                           CCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGG                                                                   >  SRR4302535.3807056/1‑94 (MQ=60)

                            CGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTG                                                                  <  SRR4302535.3151204/94‑1 (MQ=60)

                            CGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTG                                                                  <  SRR4302535.1976701/94‑1 (MQ=60)

                            CGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTG                                                                  >  SRR4302535.808120/1‑94 (MQ=60)

                            CGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTG                                                                  >  SRR4302535.3765468/1‑94 (MQ=60)

                            CGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTG                                                                  <  SRR4302535.285807/94‑1 (MQ=60)

                             GGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGA                                                                 >  SRR4302535.3354102/1‑94 (MQ=60)

                               TTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATT                                                               <  SRR4302535.4695133/94‑1 (MQ=60)

                               TTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATT                                                               <  SRR4302535.1596464/94‑1 (MQ=60)

                               TTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATG                                                               >  SRR4302535.4532561/1‑94 (MQ=60)

                                TAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTT                                                              <  SRR4302535.202502/94‑1 (MQ=60)

                                TAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTT                                                              <  SRR4302535.4683155/94‑1 (MQ=60)

                                 AGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTG                                                             <  SRR4302535.1788680/94‑1 (MQ=60)

                                 AGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTG                                                             <  SRR4302535.857709/94‑1 (MQ=60)

                                 AGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTG                                                             <  SRR4302535.3652523/94‑1 (MQ=60)

                                  GTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGTTGTCGTACATTGCTTCTGGAAAGTGATTTTA                                                            >  SRR4302535.4440033/1‑94 (MQ=60)

                                  GTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTTA                                                            >  SRR4302535.2915156/1‑94 (MQ=60)

                                  GTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGA                                                            <  SRR4302535.1544386/94‑1 (MQ=60)

                                  GTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGA                                                            >  SRR4302535.1152155/1‑94 (MQ=60)

                                     AGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCA                                                         >  SRR4302535.3628759/1‑94 (MQ=60)

                                     AGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCA                                                         >  SRR4302535.2045418/1‑94 (MQ=60)

                                      GAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAG                                                        <  SRR4302535.812426/94‑1 (MQ=60)

                                      GAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAG                                                        <  SRR4302535.1254563/94‑1 (MQ=60)

                                      GAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAG                                                        <  SRR4302535.175104/94‑1 (MQ=60)

                                         ATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTA                                                     <  SRR4302535.1718259/94‑1 (MQ=60)

                                         ATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTA                                                     >  SRR4302535.2738567/1‑94 (MQ=60)

                                            ACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACG                                                  <  SRR4302535.167844/94‑1 (MQ=60)

                                            ACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACG                                                  <  SRR4302535.360981/94‑1 (MQ=60)

                                            ACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCCCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACA                                                  >  SRR4302535.1532578/1‑94 (MQ=60)

                                             CGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGA                                                 >  SRR4302535.178924/1‑94 (MQ=60)

                                              GGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGGAAGTGATTTGAGCAGGGAACGAA                                                >  SRR4302535.1295798/1‑94 (MQ=60)

                                              GGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAA                                                >  SRR4302535.361329/1‑94 (MQ=60)

                                               GGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTTGCACATTGCTTCTGGAAAGTGATTTGGGCAGGTAACGAAT                                               >  SRR4302535.3832409/1‑94 (MQ=60)

                                               GGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAAT                                               >  SRR4302535.2846013/1‑94 (MQ=60)

                                                GAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATT                                              <  SRR4302535.2927879/94‑1 (MQ=60)

                                                 AGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGACAGTGATTTGAACAGGTAACGAATTC                                             >  SRR4302535.347921/1‑94 (MQ=60)

                                                   GCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAA                                           <  SRR4302535.2023489/94‑1 (MQ=60)

                                                   GCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAA                                           <  SRR4302535.1604306/94‑1 (MQ=60)

                                                   GCATGCTACCACTGCGAATTTAACCACTTACTGAAATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAA                                           <  SRR4302535.2972153/94‑1 (MQ=60)

                                                    CATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                          <  SRR4302535.3879045/94‑1 (MQ=60)

                                                    CATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                          >  SRR4302535.4568939/1‑94 (MQ=60)

                                                    CATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAG                                          <  SRR4302535.1049402/94‑1 (MQ=60)

                                                     ATGCTACCACTGCGAATTTAACCACTTACTGACATGACTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGC                                         <  SRR4302535.2736124/94‑1 (MQ=60)

                                                      TGGTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGATCAGGTAACGAATTCAAGCC                                        <  SRR4302535.742541/94‑1 (MQ=60)

                                                       GCTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCA                                       <  SRR4302535.1695844/94‑1 (MQ=60)

                                                        CTACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAA                                      >  SRR4302535.3944385/1‑94 (MQ=60)

                                                         TACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAAT                                     <  SRR4302535.3298581/94‑1 (MQ=60)

                                                          ACCACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATG                                    <  SRR4302535.2880122/94‑1 (MQ=60)

                                                            CACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGA                                  <  SRR4302535.2759438/94‑1 (MQ=60)

                                                             ACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAA                                 <  SRR4302535.355261/94‑1 (MQ=60)

                                                             ACTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAA                                 <  SRR4302535.3673946/94‑1 (MQ=60)

                                                              CTGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAA                                >  SRR4302535.3283707/1‑94 (MQ=60)

                                                              CTGCGAATTTAACCACTTACTGACATGGCTCGCGATGGCGCACATTGCTTCTGGTAAGGGAATTGCGCCGGCAACGAATTCAAGCCAATGGAAA                                >  SRR4302535.1188889/1‑94 (MQ=60)

                                                               TGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAAT                               <  SRR4302535.2216540/94‑1 (MQ=60)

                                                               TGCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAAT                               <  SRR4302535.218783/94‑1 (MQ=60)

                                                                GCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATA                              <  SRR4302535.1239698/94‑1 (MQ=60)

                                                                GCGAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCGGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATA                              <  SRR4302535.3115398/94‑1 (MQ=60)

                                                                  GAATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAAC                            <  SRR4302535.4627990/94‑1 (MQ=60)

                                                                   AATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACA                           >  SRR4302535.967279/1‑94 (MQ=60)

                                                                   AATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACA                           >  SRR4302535.4369211/1‑94 (MQ=60)

                                                                    ATTTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAATCCAATGGCAATAACAA                          >  SRR4302535.2498365/1‑94 (MQ=60)

                                                                      TTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAAT                        <  SRR4302535.4649152/94‑1 (MQ=60)

                                                                      TTAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAAT                        >  SRR4302535.1554363/1‑94 (MQ=60)

                                                                       TAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATA                       <  SRR4302535.1531263/94‑1 (MQ=60)

                                                                       TAACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGGAACGAATTCAAGCCAATGGAAATAACAAATA                       >  SRR4302535.2048923/1‑94 (MQ=60)

                                                                        AACCACTTACTGACATGGCTCGCGATGGCGCAGATTGCTTCTGGAAAGTGATTTGAGAAGGTAACGAATTCAAGCCAATGGAAATAACAAATAG                      <  SRR4302535.2951015/94‑1 (MQ=60)

                                                                         actctttagTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGT                     <  SRR4302535.982781/85‑1 (MQ=60)

                                                                         ACCACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGAAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGT                     <  SRR4302535.1071474/94‑1 (MQ=60)

                                                                           CACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGGAATAACAAATAGTTC                   >  SRR4302535.3187442/1‑94 (MQ=60)

                                                                           CACTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTC                   <  SRR4302535.4236588/94‑1 (MQ=60)

                                                                             CTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGC                 >  SRR4302535.3767659/1‑94 (MQ=60)

                                                                             CTTACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGC                 >  SRR4302535.3459816/1‑94 (MQ=60)

                                                                                ACTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCA              >  SRR4302535.1845901/1‑94 (MQ=60)

                                                                                 CTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAT             >  SRR4302535.1027693/1‑94 (MQ=60)

                                                                                 CTGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCAT             <  SRR4302535.3185320/94‑1 (MQ=60)

                                                                                  TGACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATG            <  SRR4302535.640215/94‑1 (MQ=60)

                                                                                   GACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGA           >  SRR4302535.2368484/1‑94 (MQ=60)

                                                                                   GACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGA           >  SRR4302535.1988177/1‑94 (MQ=60)

                                                                                   GACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGA           <  SRR4302535.972911/94‑1 (MQ=60)

                                                                                   GACATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGA           >  SRR4302535.1015369/1‑94 (MQ=60)

                                                                                     CATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACT         >  SRR4302535.3243828/1‑94 (MQ=60)

                                                                                     CATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACT         <  SRR4302535.4321529/94‑1 (MQ=60)

                                                                                      ATGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTAATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTA        >  SRR4302535.1519601/1‑94 (MQ=60)

                                                                                       TGGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT       <  SRR4302535.57692/94‑1 (MQ=60)

                                                                                       TGGCTCGCGATGTCGCACATTGATTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT       <  SRR4302535.4577279/94‑1 (MQ=60)

                                                                                       TGCCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTAT       <  SRR4302535.3019521/94‑1 (MQ=60)

                                                                                        GGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATT      >  SRR4302535.734818/1‑94 (MQ=60)

                                                                                        GGCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATT      >  SRR4302535.2625898/1‑94 (MQ=60)

                                                                                         GCTCGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTT     >  SRR4302535.717501/1‑94 (MQ=60)

                                                                                            CGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAACTAACACATAGTTCGCTCATGACTATTTTTT  >  SRR4302535.4186253/1‑94 (MQ=60)

                                                                                            CGCGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTTTT  >  SRR4302535.3743595/1‑94 (MQ=60)

CTCAATAAAAGCAGTTCCATTACTTCTCCGGTTAGTAAGAAATCACGGGAGGCATGCTACCACTGCGAATTTAACCACTTACTGACATGGCTCACGATGTCGCACATTGCTTCTGGAAAGTGATTTGAGCAGGTAACGAATTCAAGCCAATGGAAATAACAAATAGTTCGCTCATGACTATTTTTT  >  NC_000913/4227702‑4227887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`