Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,295,417 G→A P510L (CCG→CTG)  fdhF ← selenopolypeptide subunit of formate dehydrogenase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,4170GA100.0% 800.1 / NA 219P510L (CCG→CTG) fdhFselenopolypeptide subunit of formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (119/100);  total (119/100)

Only 100 of 227 total aligned reads displayed.

GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT  >  NC_000913/4295327‑4295506
                                                                                          |                                                                                         
 tCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGc                                                                                        >  2:3342423/1‑93 (MQ=255)
   cccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGcac                                                                                      >  1:996086/1‑93 (MQ=255)
   cccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGcac                                                                                      >  1:4325745/1‑93 (MQ=255)
    ccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGCAATCCAGGcaca                                                                                     >  2:807480/1‑93 (MQ=255)
     cTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAg                                                                                    >  2:2270819/1‑93 (MQ=255)
     cTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAg                                                                                    >  2:3555062/1‑93 (MQ=255)
     cTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAg                                                                                    <  1:2034935/93‑1 (MQ=255)
     cTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAg                                                                                    >  1:574572/1‑93 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   <  1:2502175/93‑1 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   <  2:4496518/93‑1 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   >  1:4475781/1‑93 (MQ=255)
       gATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAt                                                                                  >  1:4192770/1‑93 (MQ=255)
        aTCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATg                                                                                 >  2:1835089/1‑93 (MQ=255)
         tCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGa                                                                                <  1:3092442/93‑1 (MQ=255)
          cGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAc                                                                               <  1:3932890/93‑1 (MQ=255)
          cGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAc                                                                               <  2:2567847/93‑1 (MQ=255)
            gCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGc                                                                             >  2:1971526/1‑93 (MQ=255)
            gCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGc                                                                             >  2:2300388/1‑93 (MQ=255)
              aTCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCaa                                                                           >  1:1975140/1‑93 (MQ=255)
               tCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGGCGCAAc                                                                          >  2:3420620/1‑93 (MQ=255)
               tCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAc                                                                          >  1:3246501/1‑93 (MQ=255)
               tCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAc                                                                          >  1:1312415/1‑93 (MQ=255)
                 tGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTc                                                                        <  2:1315922/93‑1 (MQ=255)
                 tGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTc                                                                        >  1:50895/1‑93 (MQ=255)
                  gAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCa                                                                       >  1:3406401/1‑93 (MQ=255)
                    aGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATc                                                                     <  1:2617222/93‑1 (MQ=255)
                    aGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATc                                                                     >  2:3696937/1‑93 (MQ=255)
                      tATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATccc                                                                   >  2:2658183/1‑93 (MQ=255)
                       aTCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCa                                                                  >  1:1130716/1‑93 (MQ=255)
                          gcgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAt                                                               <  1:94013/93‑1 (MQ=255)
                          gcgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAt                                                               >  2:4405434/1‑93 (MQ=255)
                           cgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtc                                                              <  2:1237472/93‑1 (MQ=255)
                           cgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATACCAGAtc                                                              >  1:1621948/1‑93 (MQ=255)
                            ggCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtct                                                             >  2:3093620/1‑93 (MQ=255)
                             gCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtctc                                                            <  2:878135/93‑1 (MQ=255)
                             gCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtctc                                                            >  2:736178/1‑93 (MQ=255)
                              cAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTcc                                                           >  1:3839417/1‑93 (MQ=255)
                                aGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTg                                                         >  1:2819364/1‑93 (MQ=255)
                                 ggCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTgg                                                        >  2:4551987/1‑93 (MQ=255)
                                 ggCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTgg                                                        >  1:3182744/1‑93 (MQ=255)
                                    cACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGgtg                                                     >  1:3468867/1‑93 (MQ=255)
                                    cACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGgtg                                                     <  1:823555/93‑1 (MQ=255)
                                     aCTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCATCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGgtgt                                                    >  2:1448372/1‑93 (MQ=255)
                                     aCTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGgtgt                                                    >  2:433399/1‑93 (MQ=255)
                                        gaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgt                                                 >  1:4404821/1‑93 (MQ=255)
                                        gaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgt                                                 <  2:3440996/93‑1 (MQ=255)
                                        gaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgt                                                 >  1:2732650/1‑93 (MQ=255)
                                         actaaaGCCCAGTTCGCCTATTTTCTCGTAAGTAGCACCGTAGAAATCCCGGCACAGATGACGCAACTCATCCCAGATCTCCTGGTcgttgtt                                                >  2:596885/5‑93 (MQ=255)
                                         aatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgtt                                                >  2:2840198/1‑93 (MQ=255)
                                         aatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgtt                                                >  2:2350345/1‑93 (MQ=255)
                                         aatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgtt                                                <  1:2599122/93‑1 (MQ=255)
                                         aatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgtt                                                >  1:1818750/1‑93 (MQ=255)
                                           tgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgttgt                                              <  1:4319940/93‑1 (MQ=255)
                                            gaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAGATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTa                                             <  2:4643693/93‑1 (MQ=255)
                                            gaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTa                                             <  1:3949378/93‑1 (MQ=255)
                                              aGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGt                                           <  2:2018841/93‑1 (MQ=255)
                                                  cAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCAt                                       <  2:3329418/93‑1 (MQ=255)
                                                   aGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATc                                      <  1:1340520/93‑1 (MQ=255)
                                                     ttCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCgg                                    >  1:1013577/1‑93 (MQ=255)
                                                      tCGCCCATTTTCTCGTAAGTCGCTCCGTAGAAATCCAGGCACAGATGACGCATCTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGa                                   <  2:4560874/93‑1 (MQ=255)
                                                      tCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGa                                   <  2:2159662/93‑1 (MQ=255)
                                                       cGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGAt                                  <  2:1497676/93‑1 (MQ=255)
                                                         cccATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATaa                                >  2:3306035/1‑93 (MQ=255)
                                                          ccATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAAc                               <  1:862196/93‑1 (MQ=255)
                                                           cATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGCTCTCCTGGGTGTTGTTGTAGTGCATCGGATAAcc                              >  2:3022613/1‑93 (MQ=255)
                                                            aTTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAAccc                             <  2:1685989/93‑1 (MQ=255)
                                                             ttttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCa                            >  1:2351179/1‑93 (MQ=255)
                                                             ttttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCa                            <  1:4599438/93‑1 (MQ=255)
                                                              tttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCAt                           <  2:2942222/93‑1 (MQ=255)
                                                               ttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCAtc                          >  1:3598491/1‑92 (MQ=255)
                                                               ttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATa                          >  1:2050561/1‑93 (MQ=255)
                                                                 ctcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACg                        <  2:359176/93‑1 (MQ=255)
                                                                  tcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACgg                       >  2:1723628/1‑93 (MQ=255)
                                                                  tcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACgg                       <  1:4111607/93‑1 (MQ=255)
                                                                    gTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACgggg                     >  1:4008917/1‑92 (MQ=255)
                                                                    gTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGt                     <  1:1863375/93‑1 (MQ=255)
                                                                    gTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGt                     >  2:4213528/1‑93 (MQ=255)
                                                                       aGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                  <  1:1709544/93‑1 (MQ=255)
                                                                       aGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                  <  2:1957367/93‑1 (MQ=255)
                                                                         tCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGa                >  1:1391280/1‑93 (MQ=255)
                                                                           gCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGAtt              >  1:251863/1‑93 (MQ=255)
                                                                           gCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGAGTCGAtt              >  1:1502860/1‑93 (MQ=255)
                                                                            cACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGAttt             >  2:2662235/1‑93 (MQ=255)
                                                                              ccGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCa           >  2:2626001/1‑93 (MQ=255)
                                                                               cGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCAc          <  2:497932/93‑1 (MQ=255)
                                                                                gTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACt         <  1:2659499/93‑1 (MQ=255)
                                                                                gTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACt         >  1:2451363/1‑93 (MQ=255)
                                                                                gTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACt         >  2:3921031/1‑93 (MQ=255)
                                                                                gTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACt         >  2:3659739/1‑93 (MQ=255)
                                                                                gTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACACATACGGGAGGGGATTTCatt         >  2:4009936/1‑91 (MQ=255)
                                                                                 tAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtg        >  2:4201741/1‑93 (MQ=255)
                                                                                 tAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtg        >  2:4118693/1‑93 (MQ=255)
                                                                                 tAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtg        >  2:3252684/1‑93 (MQ=255)
                                                                                  aGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtga       <  1:1118770/93‑1 (MQ=255)
                                                                                   gAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgat      >  2:640188/1‑93 (MQ=255)
                                                                                     aaTCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgatga    >  2:1694585/1‑93 (MQ=255)
                                                                                      aTCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTgatgat   >  2:3184157/1‑93 (MQ=255)
                                                                                      aTCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTgatgat   <  2:1531029/93‑1 (MQ=255)
                                                                                       tCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAtt  >  2:3853266/1‑93 (MQ=255)
                                                                                       tCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAtt  <  1:1167789/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT  >  NC_000913/4295327‑4295506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  NC_000913/4295324‑4295510
                                                                                             |                                                                                             
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCA                                                                                               <  SRR4302535.4049873/94‑1 (MQ=60)
  AGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGG                                                                                             <  SRR4302535.3863124/94‑1 (MQ=60)
  AGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGG                                                                                             <  SRR4302535.3013976/94‑1 (MQ=60)
  AGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGG                                                                                             >  SRR4302535.1688910/1‑94 (MQ=60)
   GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGC                                                                                            >  SRR4302535.3248458/1‑94 (MQ=60)
    TCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCA                                                                                           <  SRR4302535.2750355/94‑1 (MQ=60)
     CCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCAC                                                                                          >  SRR4302535.1003159/1‑94 (MQ=60)
     CCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCAC                                                                                          >  SRR4302535.4355326/1‑94 (MQ=60)
     CCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCAC                                                                                          >  SRR4302535.1542059/1‑94 (MQ=60)
      CCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACA                                                                                         >  SRR4302535.1850792/1‑94 (MQ=60)
       CCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAG                                                                                        >  SRR4302535.578605/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302535.4368715/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302535.2051200/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       <  SRR4302535.2049595/94‑1 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302535.4506498/1‑94 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      <  SRR4302535.1333553/94‑1 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      <  SRR4302535.2520219/94‑1 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      >  SRR4302535.4221462/1‑94 (MQ=60)
            TCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAC                                                                                   <  SRR4302535.2463807/94‑1 (MQ=60)
            TCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAC                                                                                   <  SRR4302535.4349979/94‑1 (MQ=60)
            TCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAC                                                                                   <  SRR4302535.3113977/94‑1 (MQ=60)
             CGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACG                                                                                  >  SRR4302535.4059388/1‑94 (MQ=60)
             CGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACG                                                                                  <  SRR4302535.3959734/94‑1 (MQ=60)
                CATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAA                                                                               >  SRR4302535.1989367/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302535.3269045/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302535.3602382/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302535.1321797/1‑94 (MQ=60)
                   CTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTC                                                                            >  SRR4302535.51269/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302535.1892788/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302535.3430109/1‑94 (MQ=60)
                     GAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAT                                                                          <  SRR4302535.4709675/94‑1 (MQ=60)
                     GAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAT                                                                          <  SRR4302535.4678958/94‑1 (MQ=60)
                     GAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAT                                                                          >  SRR4302535.873379/1‑94 (MQ=60)
                       AGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCC                                                                        <  SRR4302535.2635984/94‑1 (MQ=60)
                        GTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCC                                                                       <  SRR4302535.449240/94‑1 (MQ=60)
                         TATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      >  SRR4302535.1138787/1‑94 (MQ=60)
                         TATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      >  SRR4302535.748703/1‑94 (MQ=60)
                         TATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAACTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      <  SRR4302535.2535031/94‑1 (MQ=60)
                          ATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAG                                                                     >  SRR4302535.4038030/1‑94 (MQ=60)
                            CGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAT                                                                   <  SRR4302535.3968505/94‑1 (MQ=60)
                             GCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATC                                                                  <  SRR4302535.94680/94‑1 (MQ=60)
                             GCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATC                                                                  <  SRR4302535.2831085/94‑1 (MQ=60)
                             GCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATACCAGATC                                                                  >  SRR4302535.1633656/1‑94 (MQ=60)
                                GCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCC                                                               >  SRR4302535.3865880/1‑94 (MQ=60)
                                 CAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCT                                                              >  SRR4302535.845613/1‑94 (MQ=60)
                                 CAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCT                                                              <  SRR4302535.174740/94‑1 (MQ=60)
                                  AAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTG                                                             >  SRR4302535.2839388/1‑94 (MQ=60)
                                  AAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTG                                                             >  SRR4302535.1131475/1‑94 (MQ=60)
                                   AGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGG                                                            >  SRR4302535.4391810/1‑94 (MQ=60)
                                   AGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGG                                                            >  SRR4302535.3204736/1‑94 (MQ=60)
                                   AGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGG                                                            <  SRR4302535.2023801/94‑1 (MQ=60)
                                    GGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGG                                                           <  SRR4302535.3251459/94‑1 (MQ=60)
                                     GCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGT                                                          >  SRR4302535.2618838/1‑94 (MQ=60)
                                      CCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTG                                                         >  SRR4302535.3492972/1‑94 (MQ=60)
                                      CCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTG                                                         <  SRR4302535.2590935/94‑1 (MQ=60)
                                       CACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGT                                                        <  SRR4302535.829328/94‑1 (MQ=60)
                                        ACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTT                                                       <  SRR4302535.1505752/94‑1 (MQ=60)
                                          TGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGT                                                     >  SRR4302535.2752155/1‑94 (MQ=60)
                                          TGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGT                                                     >  SRR4302535.4434966/1‑94 (MQ=60)
                                           GAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTT                                                    >  SRR4302535.1831894/1‑94 (MQ=60)
                                           GAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTT                                                    <  SRR4302535.2209359/94‑1 (MQ=60)
                                           GAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTT                                                    <  SRR4302535.3188457/94‑1 (MQ=60)
                                            AATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTG                                                   <  SRR4302535.2617783/94‑1 (MQ=60)
                                              TGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTA                                                 <  SRR4302535.4349491/94‑1 (MQ=60)
                                               GAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAG                                                <  SRR4302535.3976342/94‑1 (MQ=60)
                                               GAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAG                                                <  SRR4302535.249876/94‑1 (MQ=60)
                                                 AGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTG                                              <  SRR4302535.1153788/94‑1 (MQ=60)
                                                 AGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTG                                              <  SRR4302535.896025/94‑1 (MQ=60)
                                                 AGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTG                                              <  SRR4302535.2837205/94‑1 (MQ=60)
                                                  GCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGC                                             <  SRR4302535.515036/94‑1 (MQ=60)
                                                    CCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCAT                                           <  SRR4302535.3307943/94‑1 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCACCTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         <  SRR4302535.427026/94‑1 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         <  SRR4302535.1350083/94‑1 (MQ=60)
                                                       GTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGG                                        >  SRR4302535.1020784/1‑94 (MQ=60)
                                                       GTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGG                                        >  SRR4302535.3696402/1‑94 (MQ=60)
                                                         TCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGAT                                      <  SRR4302535.3054784/94‑1 (MQ=60)
                                                          CGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATA                                     <  SRR4302535.3930401/94‑1 (MQ=60)
                                                             CCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACC                                  <  SRR4302535.868291/94‑1 (MQ=60)
                                                               ATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCA                                >  SRR4302535.2368035/1‑94 (MQ=60)
                                                                TTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCAT                               <  SRR4302535.4630724/94‑1 (MQ=60)
                                                                 TTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATC                              >  SRR4302535.3623463/1‑94 (MQ=60)
                                                                 TTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATA                              >  SRR4302535.2065327/1‑94 (MQ=60)
                                                                   TCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCATCTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACG                            <  SRR4302535.2296280/94‑1 (MQ=60)
                                                                     TCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGG                          <  SRR4302535.997763/94‑1 (MQ=60)
                                                                     TCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGG                          <  SRR4302535.4139749/94‑1 (MQ=60)
                                                                      CGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGG                         >  SRR4302535.4036340/1‑94 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        <  SRR4302535.3467356/94‑1 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        <  SRR4302535.4016232/94‑1 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        <  SRR4302535.1876809/94‑1 (MQ=60)
                                                                          AGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCG                     <  SRR4302535.1721905/94‑1 (MQ=60)
                                                                          AGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCG                     >  SRR4302535.4568929/1‑94 (MQ=60)
                                                                          AGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCG                     >  SRR4302535.3746377/1‑94 (MQ=60)
                                                                           GTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGA                    >  SRR4302535.1401211/1‑94 (MQ=60)
                                                                            TCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGAT                   >  SRR4302535.4178449/1‑94 (MQ=60)
                                                                             CGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATT                  >  SRR4302535.253597/1‑94 (MQ=60)
                                                                             CGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATT                  >  SRR4302535.1197260/1‑94 (MQ=60)
                                                                             CGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGAGTCGATT                  >  SRR4302535.1513673/1‑94 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             >  SRR4302535.2469032/1‑94 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             >  SRR4302535.1257760/1‑94 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGGGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             >  SRR4302535.259744/1‑94 (MQ=60)
                                                                                   GTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTG            <  SRR4302535.2669729/94‑1 (MQ=60)
                                                                                   GTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTG            <  SRR4302535.2678552/94‑1 (MQ=60)
                                                                                     AGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGAT          <  SRR4302535.1126751/94‑1 (MQ=60)
                                                                                     AGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGAT          <  SRR4302535.1351903/94‑1 (MQ=60)
                                                                                      GAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATG         <  SRR4302535.1093862/94‑1 (MQ=60)
                                                                                         ATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT      <  SRR4302535.1570688/94‑1 (MQ=60)
                                                                                         ATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT      >  SRR4302535.1963240/1‑94 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     >  SRR4302535.2833675/1‑94 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     >  SRR4302535.2970144/1‑94 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     <  SRR4302535.1176126/94‑1 (MQ=60)
                                                                                           CCAGGCACAGATGACGCAACTCATCCCAGGTCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTG    <  SRR4302535.3024149/94‑1 (MQ=60)
                                                                                             AGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  SRR4302535.2713159/1‑94 (MQ=60)
                                                                                             AGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  SRR4302535.2671682/1‑94 (MQ=60)
                                                                                             AGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  SRR4302535.2508165/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  NC_000913/4295324‑4295510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑