Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,333,152 A→G L16P (CTA→CCA)  yjdB ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,333,1520AG99.1% 703.6 / ‑5.3 226L16P (CTA→CCA) yjdBhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (122/102);  total (124/102)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGT  >  NC_000913/4333062‑4333241
                                                                                          |                                                                                         
acCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTtggt                                                                                         >  1:951173/1‑93 (MQ=255)
 ccTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTtggta                                                                                        <  1:1936790/93‑1 (MQ=255)
     tttAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGgcg                                                                                    >  2:3001944/1‑93 (MQ=255)
     tttAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAAATCAAAGAGGGTCTTTTTAGTAGgcg                                                                                    >  1:2473495/1‑93 (MQ=255)
        aaaaaaGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGGCTTTTTGGTAGGCGCtt                                                                                 >  2:2821229/1‑93 (MQ=255)
          aaaaGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCa                                                                               >  1:1042725/1‑93 (MQ=255)
          aaaaGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCa                                                                               >  1:921059/1‑93 (MQ=255)
           aaaGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCaa                                                                              <  2:4233488/93‑1 (MQ=255)
           aaaGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCaa                                                                              <  2:3410138/93‑1 (MQ=255)
           aaaGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCaa                                                                              <  1:3226443/93‑1 (MQ=255)
            aaGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAAc                                                                             <  1:2376461/93‑1 (MQ=255)
             aGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATCGCCAGGCGAGCAAATTCAAAGAGGGACTTTTAGGTAGGCGCCTAAACa                                                                            >  2:3359849/1‑93 (MQ=255)
             aGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACa                                                                            >  1:3839175/1‑93 (MQ=255)
             aGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACa                                                                            >  1:3777561/1‑93 (MQ=255)
              ggCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACAt                                                                           >  2:1273830/1‑93 (MQ=255)
                cAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCa                                                                         <  1:319914/93‑1 (MQ=255)
                 aaTATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCAc                                                                        <  1:2917620/93‑1 (MQ=255)
                 aaTATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCAc                                                                        <  1:2839947/93‑1 (MQ=255)
                  atatTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACg                                                                       >  2:2250608/1‑93 (MQ=255)
                  atatTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGATCTTCAACATCACg                                                                       >  1:1623662/1‑93 (MQ=255)
                   tatTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACgg                                                                      <  2:4338488/93‑1 (MQ=255)
                   tatTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACgg                                                                      <  2:2777905/93‑1 (MQ=255)
                     ttCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGgtg                                                                    <  1:177716/93‑1 (MQ=255)
                      tCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGgtgt                                                                   <  1:3539797/93‑1 (MQ=255)
                        aGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGttt                                                                 >  2:3806653/1‑93 (MQ=255)
                          gCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTca                                                               >  1:1261414/1‑92 (MQ=255)
                           cAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCa                                                              <  1:3629453/93‑1 (MQ=255)
                             gATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATc                                                            <  2:3201967/93‑1 (MQ=255)
                             gATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATc                                                            <  2:182346/93‑1 (MQ=255)
                              aTAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCg                                                           >  1:3377703/1‑93 (MQ=255)
                                 gagaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAAc                                                        >  2:4031892/1‑93 (MQ=255)
                                 gagaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAAc                                                        >  2:654296/1‑93 (MQ=255)
                                  agaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACa                                                       <  2:1193764/93‑1 (MQ=255)
                                   gaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACaa                                                      >  1:2030029/1‑93 (MQ=255)
                                   gaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACaa                                                      >  1:1954311/1‑93 (MQ=255)
                                     tataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAg                                                    >  2:811459/1‑93 (MQ=255)
                                     tataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGCGTTTCCATCGAACAAAg                                                    >  2:2723251/1‑93 (MQ=255)
                                     tataAAAAGCGGCCAACAATAGCCAGGCGAGAAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGCTGTTTCCATCGCACAAAg                                                    >  2:3783261/1‑93 (MQ=255)
                                      ataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGt                                                   >  1:2414705/1‑93 (MQ=255)
                                      ataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGt                                                   <  2:4647714/93‑1 (MQ=255)
                                      ataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGt                                                   >  2:501672/1‑93 (MQ=255)
                                      agaaaaaGCGTCGAACAATAGCCAGGCGAGTAAATGCAAAGAGGGTCTTTTTGGTAGGCGCTTCACCATCACGGTGTTTCCATCGAACAAAGt                                                   <  2:2509777/91‑1 (MQ=255)
                                         aaaaGGGGCCAACAATAGCCAGGTGAGGGAGTTCAAAGAGGGTCTTTTTGGTAGGCCATTCAACATCACGGCGTTTCCATCGAACAAAGTgcg                                                >  2:2149697/1‑93 (MQ=255)
                                         aaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTgcg                                                >  2:1014418/1‑93 (MQ=255)
                                         aaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTgcg                                                >  2:438112/1‑93 (MQ=255)
                                           aaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCa                                              <  1:1701233/93‑1 (MQ=255)
                                           aaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCa                                              >  1:4293926/1‑93 (MQ=255)
                                           aaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCa                                              <  2:3873716/93‑1 (MQ=255)
                                             gCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCata                                            <  2:512646/93‑1 (MQ=255)
                                             gCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCata                                            <  2:3485830/93‑1 (MQ=255)
                                             gCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCata                                            >  1:143348/1‑93 (MQ=255)
                                              cGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCatat                                           >  1:1842240/1‑93 (MQ=255)
                                               ggCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCATTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATg                                          <  1:2127901/93‑1 (MQ=255)
                                                 ccAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCt                                        <  1:488673/93‑1 (MQ=255)
                                                  caacaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTc                                       <  1:1003568/93‑1 (MQ=255)
                                                   aacaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCg                                      >  1:2759425/1‑93 (MQ=255)
                                                    acaaTAGCCAGGCGAGTAAATTCAAAGAGGGGCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGGATATGCTCgc                                     >  2:2507862/1‑93 (MQ=255)
                                                     caaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgca                                    >  2:753717/1‑93 (MQ=255)
                                                      aaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaa                                   >  2:153696/1‑93 (MQ=255)
                                                       aTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaa                                  >  1:3759511/1‑93 (MQ=255)
                                                       aTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaa                                  >  2:1997335/1‑93 (MQ=255)
                                                       aTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaa                                  <  2:1615266/93‑1 (MQ=255)
                                                        tAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaag                                 >  1:645960/1‑93 (MQ=255)
                                                        tAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaag                                 >  2:1899047/1‑93 (MQ=255)
                                                        tAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaag                                 >  2:1586876/1‑93 (MQ=255)
                                                        tAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTAGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaag                                 <  2:2023956/93‑1 (MQ=255)
                                                          gCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaagca                               >  1:2888847/1‑93 (MQ=255)
                                                            cAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaagcaaa                             >  1:2093592/1‑93 (MQ=255)
                                                            cAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCgcaaagcaaa                             >  1:3107486/1‑93 (MQ=255)
                                                             aGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCaaaa                            >  2:1694737/1‑93 (MQ=255)
                                                             aGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCaaaa                            <  1:82153/93‑1 (MQ=255)
                                                              ggCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAAt                           <  2:3745495/93‑1 (MQ=255)
                                                              ggCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAAt                           >  1:4536271/1‑93 (MQ=255)
                                                                cGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTa                         <  2:2827054/93‑1 (MQ=255)
                                                                cGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTa                         <  2:3952197/93‑1 (MQ=255)
                                                                 gAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTaa                        >  2:3624749/1‑93 (MQ=255)
                                                                  aGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAg                       >  1:4157572/1‑93 (MQ=255)
                                                                   gTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGc                      <  2:2852112/93‑1 (MQ=255)
                                                                   gTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGCTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGc                      <  1:3044249/93‑1 (MQ=255)
                                                                    tAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGcc                     >  1:4266737/1‑93 (MQ=255)
                                                                      aaTTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCac                   >  2:2604451/1‑92 (MQ=255)
                                                                       aTTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAAc                  <  1:2908129/93‑1 (MQ=255)
                                                                       aTTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAAc                  >  1:4557694/1‑93 (MQ=255)
                                                                       aTTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAAc                  <  2:5927/93‑1 (MQ=255)
                                                                        ttCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAAcc                 >  1:1663845/1‑93 (MQ=255)
                                                                           aaaGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTa              >  2:2102946/1‑93 (MQ=255)
                                                                            aaGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTaa             <  2:3194416/93‑1 (MQ=255)
                                                                            aaGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTaa             >  2:1475008/1‑93 (MQ=255)
                                                                              gagGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGt           <  2:3310397/93‑1 (MQ=255)
                                                                              gagGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGt           <  1:2700190/93‑1 (MQ=255)
                                                                              gagGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGt           <  2:695347/93‑1 (MQ=255)
                                                                               agGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGtt          <  2:3263186/93‑1 (MQ=255)
                                                                                gggTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTc         <  1:4004610/93‑1 (MQ=255)
                                                                                   tCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTa      >  1:4222908/1‑93 (MQ=255)
                                                                                    ttttttGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTaa     <  1:1715168/92‑1 (MQ=255)
                                                                                    cTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTaa     <  1:2727961/93‑1 (MQ=255)
                                                                                    cTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTaa     <  2:2957014/93‑1 (MQ=255)
                                                                                     tttttGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAg    <  2:4464382/93‑1 (MQ=255)
                                                                                      ttttGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAgg   >  2:1676886/1‑93 (MQ=255)
                                                                                       tttGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGt  <  1:851870/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGT  >  NC_000913/4333062‑4333241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGG  >  NC_000913/4333059‑4333244
                                                                                             |                                                                                            
AACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTG                                                                                              >  SRR4302535.306578/1‑94 (MQ=60)
 ACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGG                                                                                             <  SRR4302535.1457443/94‑1 (MQ=60)
  CACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGT                                                                                            >  SRR4302535.957905/1‑94 (MQ=60)
   ACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTA                                                                                           <  SRR4302535.3202182/94‑1 (MQ=60)
    CCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAG                                                                                          >  SRR4302535.2498657/1‑94 (MQ=60)
    CCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAG                                                                                          <  SRR4302535.1950736/94‑1 (MQ=60)
     CTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGG                                                                                         >  SRR4302535.1322771/1‑94 (MQ=60)
      TGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGC                                                                                        >  SRR4302535.4282209/1‑94 (MQ=60)
       GTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAAATCAAAGAGGGTCTTTTTAGTAGGCG                                                                                       >  SRR4302535.2491336/1‑94 (MQ=60)
        TTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGC                                                                                      >  SRR4302535.663024/1‑94 (MQ=60)
         TTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCT                                                                                     >  SRR4302535.1532780/1‑94 (MQ=60)
         TTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCT                                                                                     <  SRR4302535.3246946/94‑1 (MQ=60)
            AAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCA                                                                                  >  SRR4302535.1050117/1‑94 (MQ=60)
            AAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCA                                                                                  >  SRR4302535.927591/1‑94 (MQ=60)
            AAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCA                                                                                  >  SRR4302535.4212787/1‑94 (MQ=60)
             AAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAA                                                                                 >  SRR4302535.2109898/1‑94 (MQ=60)
              AAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAAC                                                                                <  SRR4302535.3248809/94‑1 (MQ=60)
              AAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAAC                                                                                <  SRR4302535.2067788/94‑1 (MQ=60)
              AAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAAC                                                                                >  SRR4302535.4601002/1‑94 (MQ=60)
               AAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACA                                                                               >  SRR4302535.3865636/1‑94 (MQ=60)
               AAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACA                                                                               <  SRR4302535.2393533/94‑1 (MQ=60)
               AAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACA                                                                               <  SRR4302535.2220104/94‑1 (MQ=60)
               AAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACA                                                                               >  SRR4302535.3803600/1‑94 (MQ=60)
               AAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACA                                                                               >  SRR4302535.545673/1‑94 (MQ=60)
                AGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACAT                                                                              <  SRR4302535.4011570/94‑1 (MQ=60)
                   CAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCAC                                                                           <  SRR4302535.322128/94‑1 (MQ=60)
                    AATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACG                                                                          <  SRR4302535.2938214/94‑1 (MQ=60)
                    AATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACG                                                                          <  SRR4302535.2860092/94‑1 (MQ=60)
                    AATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACG                                                                          >  SRR4302535.1780610/1‑94 (MQ=60)
                    AATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGATCTTCAACATCACG                                                                          >  SRR4302535.1635385/1‑94 (MQ=60)
                     ATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGG                                                                         >  SRR4302535.1692703/1‑94 (MQ=60)
                     ATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGG                                                                         >  SRR4302535.2971583/1‑94 (MQ=60)
                     ATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGG                                                                         >  SRR4302535.3824812/1‑94 (MQ=60)
                     ATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGG                                                                         >  SRR4302535.3483962/1‑94 (MQ=60)
                      TATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGT                                                                        >  SRR4302535.4659789/1‑94 (MQ=60)
                       ATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTG                                                                       <  SRR4302535.4320193/94‑1 (MQ=60)
                       ATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTG                                                                       >  SRR4302535.957602/1‑94 (MQ=60)
                       ATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGCCTTTTTGGTAGGCGCTTCAACATCACGGCG                                                                       >  SRR4302535.2093274/1‑94 (MQ=60)
                        TTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGT                                                                      <  SRR4302535.178951/94‑1 (MQ=60)
                         TCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTT                                                                     <  SRR4302535.3564394/94‑1 (MQ=60)
                            GGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCC                                                                  <  SRR4302535.1651013/94‑1 (MQ=60)
                            GGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCA                                                                  >  SRR4302535.1270439/1‑94 (MQ=60)
                              CAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCAT                                                                <  SRR4302535.3654610/94‑1 (MQ=60)
                                GATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCG                                                              >  SRR4302535.3401189/1‑94 (MQ=60)
                                    GAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACA                                                          <  SRR4302535.945303/94‑1 (MQ=60)
                                     AGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAA                                                         >  SRR4302535.2044660/1‑94 (MQ=60)
                                     AGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAA                                                         >  SRR4302535.1968376/1‑94 (MQ=60)
                                       ATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAG                                                       <  SRR4302535.2166976/94‑1 (MQ=60)
                                       ATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAG                                                       >  SRR4302535.1844237/1‑94 (MQ=60)
                                        TATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGT                                                      >  SRR4302535.2432088/1‑94 (MQ=60)
                                        TATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGT                                                      >  SRR4302535.1988077/1‑94 (MQ=60)
                                        TATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGT                                                      >  SRR4302535.2900103/1‑94 (MQ=60)
                                           AAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCG                                                   <  SRR4302535.2872023/94‑1 (MQ=60)
                                           AAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCG                                                   >  SRR4302535.4426987/1‑94 (MQ=60)
                                           AAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCG                                                   <  SRR4302535.4544131/94‑1 (MQ=60)
                                           AAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTGTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCG                                                   <  SRR4302535.1002014/94‑1 (MQ=60)
                                            AAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGC                                                  >  SRR4302535.4018337/1‑94 (MQ=60)
                                             AAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCA                                                 <  SRR4302535.2477439/94‑1 (MQ=60)
                                             AAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCA                                                 >  SRR4302535.4323306/1‑94 (MQ=60)
                                             AAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCA                                                 <  SRR4302535.1957851/94‑1 (MQ=60)
                                              AAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCAT                                                <  SRR4302535.1734/94‑1 (MQ=60)
                                              AAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCAT                                                <  SRR4302535.1713528/94‑1 (MQ=60)
                                               AGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATA                                               >  SRR4302535.144345/1‑94 (MQ=60)
                                                GCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATAT                                              <  SRR4302535.2930145/94‑1 (MQ=60)
                                                GCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATAT                                              >  SRR4302535.1855527/1‑94 (MQ=60)
                                                 CGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGTATATG                                             >  SRR4302535.2765817/1‑94 (MQ=60)
                                                 CGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATG                                             <  SRR4302535.896752/94‑1 (MQ=60)
                                                  GGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGC                                            >  SRR4302535.1012564/1‑94 (MQ=60)
                                                  GGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCATTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGC                                            <  SRR4302535.2143245/94‑1 (MQ=60)
                                                    CCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTC                                          >  SRR4302535.409727/1‑94 (MQ=60)
                                                    CCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTC                                          <  SRR4302535.242133/94‑1 (MQ=60)
                                                    CCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTC                                          <  SRR4302535.492098/94‑1 (MQ=60)
                                                     CAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCG                                         <  SRR4302535.1010692/94‑1 (MQ=60)
                                                     CAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCG                                         >  SRR4302535.2779089/1‑94 (MQ=60)
                                                       ACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCA                                       >  SRR4302535.2792042/1‑94 (MQ=60)
                                                         AATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAA                                     >  SRR4302535.3785498/1‑94 (MQ=60)
                                                          ATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAG                                    >  SRR4302535.650467/1‑94 (MQ=60)
                                                            AGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCA                                  >  SRR4302535.2909284/1‑94 (MQ=60)
                                                            AGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCA                                  <  SRR4302535.2737119/94‑1 (MQ=60)
                                                             GCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAA                                 >  SRR4302535.333385/1‑94 (MQ=60)
                                                              CCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA                                >  SRR4302535.2108683/1‑94 (MQ=60)
                                                              CCAGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA                                >  SRR4302535.3129121/1‑94 (MQ=60)
                                                                AGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAAT                              >  SRR4302535.4567307/1‑94 (MQ=60)
                                                                AGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAAT                              >  SRR4302535.749879/1‑94 (MQ=60)
                                                                AGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAAT                              <  SRR4302535.82737/94‑1 (MQ=60)
                                                                AGGCGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAAT                              >  SRR4302535.3091446/1‑94 (MQ=60)
                                                                   CGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAA                           <  SRR4302535.3134516/94‑1 (MQ=60)
                                                                   CGAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAA                           >  SRR4302535.2913391/1‑94 (MQ=60)
                                                                    GAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAG                          >  SRR4302535.4186032/1‑94 (MQ=60)
                                                                    GAGTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAG                          >  SRR4302535.2690033/1‑94 (MQ=60)
                                                                     AGTAAATTCAAAGAGGCTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGC                         <  SRR4302535.1941183/94‑1 (MQ=60)
                                                                      GTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCC                        >  SRR4302535.4194843/1‑94 (MQ=60)
                                                                      GTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCC                        >  SRR4302535.4295952/1‑94 (MQ=60)
                                                                      GTAAATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGCTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCC                        <  SRR4302535.3065574/94‑1 (MQ=60)
                                                                         AATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAAC                     >  SRR4302535.4588814/1‑94 (MQ=60)
                                                                         AATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAAC                     <  SRR4302535.2751416/94‑1 (MQ=60)
                                                                          ATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACC                    <  SRR4302535.2928685/94‑1 (MQ=60)
                                                                          ATTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACC                    >  SRR4302535.1675869/1‑94 (MQ=60)
                                                                           TTCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCT                   >  SRR4302535.84389/1‑94 (MQ=60)
                                                                            TCAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTT                  >  SRR4302535.3664414/1‑94 (MQ=60)
                                                                             CGAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTA                 >  SRR4302535.1644100/1‑94 (MQ=60)
                                                                             CAAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTA                 >  SRR4302535.3636959/1‑94 (MQ=60)
                                                                              AAAGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAA                <  SRR4302535.3017774/94‑1 (MQ=60)
                                                                                AGAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGT              >  SRR4302535.1937697/1‑94 (MQ=60)
                                                                                 GAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTT             <  SRR4302535.4569514/94‑1 (MQ=60)
                                                                                 GAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTT             <  SRR4302535.2719485/94‑1 (MQ=60)
                                                                                 GAGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTT             <  SRR4302535.1275919/94‑1 (MQ=60)
                                                                                  AGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTC            >  SRR4302535.3528470/1‑94 (MQ=60)
                                                                                  AGGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTC            >  SRR4302535.3728770/1‑94 (MQ=60)
                                                                                   GGGTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCT           <  SRR4302535.4032005/94‑1 (MQ=60)
                                                                                     GTCTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTA         >  SRR4302535.4251802/1‑94 (MQ=60)
                                                                                       TTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAG       <  SRR4302535.1727571/94‑1 (MQ=60)
                                                                                       CTTTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAG       <  SRR4302535.2747441/94‑1 (MQ=60)
                                                                                         TTTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGT     >  SRR4302535.3800854/1‑94 (MQ=60)
                                                                                          TTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTT    <  SRR4302535.857869/94‑1 (MQ=60)
                                                                                          TTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTT    <  SRR4302535.1081737/94‑1 (MQ=60)
                                                                                          TTTGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTT    <  SRR4302535.1731017/94‑1 (MQ=60)
                                                                                            TGGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGG  <  SRR4302535.2520908/94‑1 (MQ=60)
                                                                                             |                                                                                            
AACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGG  >  NC_000913/4333059‑4333244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑