Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,369,183	A→G	Q194R (CAG→CGG)	mopA →	GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,369,183	0	A	G	99.5%	655.0 / ‑4.9	211	Q194R (CAG→CGG)	mopA	GroEL
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (107/102); total (108/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 220 total aligned reads displayed.

AAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTG  >  NC_000913/4369093‑4369272

 aGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGtt                                                                                        >  2:692632/1‑93 (MQ=255)

  gCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTc                                                                                       >  2:4460443/1‑93 (MQ=255)

   cGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCg                                                                                      <  1:2438079/93‑1 (MQ=255)

    gATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGa                                                                                     >  2:4395399/1‑93 (MQ=255)

    gATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGa                                                                                     <  2:321874/93‑1 (MQ=255)

    gATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGa                                                                                     >  1:2237416/1‑93 (MQ=255)

       ggACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCg                                                                                  >  2:781395/1‑93 (MQ=255)

        gACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGt                                                                                 >  2:2959585/1‑93 (MQ=255)

         aCAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTg                                                                                >  1:575597/1‑93 (MQ=255)

          cAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAAGTATGCGGTTCGACCGTgg                                                                               <  2:4554016/93‑1 (MQ=255)

            aaGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCt                                                                             >  2:919584/1‑93 (MQ=255)

            aaGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCt                                                                             >  1:3160534/1‑93 (MQ=255)

             aGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTa                                                                            <  2:3615031/93‑1 (MQ=255)

             aGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTa                                                                            >  1:805632/1‑93 (MQ=255)

             aGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTa                                                                            >  1:2302862/1‑93 (MQ=255)

             aGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTa                                                                            <  2:2609860/93‑1 (MQ=255)

              gTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTAc                                                                           <  1:4069092/93‑1 (MQ=255)

               tCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTAcc                                                                          >  2:1341390/1‑93 (MQ=255)

                 ggTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTg                                                                        >  2:184564/1‑93 (MQ=255)

                  gaaaaGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGAACGAACTGGGCGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGt                                                                       <  2:4332723/91‑1 (MQ=255)

                  gTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGt                                                                       >  2:2317004/1‑93 (MQ=255)

                   tAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGtc                                                                      <  2:1657790/93‑1 (MQ=255)

                    aaaGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGtct                                                                     <  1:4336705/93‑1 (MQ=255)

                     aagaagGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGtctc                                                                    >  1:4282845/1‑93 (MQ=255)

                      agaagGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTcc                                                                   <  2:2907359/93‑1 (MQ=255)

                      agaagGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTcc                                                                   >  2:2371009/1‑93 (MQ=255)

                      agaagGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTcc                                                                   >  2:1585975/1‑93 (MQ=255)

                       gaagGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCt                                                                  <  2:2741310/93‑1 (MQ=255)

                       gaagGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCt                                                                  >  1:534285/1‑93 (MQ=255)

                       gaagGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCt                                                                  <  2:1401813/93‑1 (MQ=255)

                       gaagGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCt                                                                  >  2:1908635/1‑93 (MQ=255)

                         agGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTa                                                                <  2:3589524/93‑1 (MQ=255)

                          ggCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTAc                                                               >  2:1425236/1‑93 (MQ=255)

                            cGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACtt                                                             <  1:1784227/93‑1 (MQ=255)

                             gTTATCGCCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTtc                                                            <  2:1775693/93‑1 (MQ=255)

                             gTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCCACCGTGGCTACCTGTCTCCTTACTtc                                                            >  1:2036471/1‑93 (MQ=255)

                             gTTATCACCGTTGAAGACGGTACCCGTCTGCAGGACGCACTGGACGCGGTTGAAGGTATGCGGTTCGCCCGTGGCTACCTGTCTCCTTACTtc                                                            >  2:622773/1‑93 (MQ=255)

                              ttATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTtca                                                           <  2:3763943/93‑1 (MQ=255)

                                 tCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTtcatca                                                        >  2:3798726/1‑93 (MQ=255)

                                 tCACCGTTGAAGAAGGCTCTGGCCTGGAAAACGAACTGTCGGTTGTAGAAGGCATGCAGTTCGACCGTGGCTACCTGTCCCCATACTtcgtca                                                        >  1:1147022/1‑93 (MQ=255)

                                  cACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATcaa                                                       <  2:950059/93‑1 (MQ=255)

                                  cACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATcaa                                                       >  1:1608744/1‑93 (MQ=255)

                                   aCCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATcaac                                                      >  2:3129059/1‑93 (MQ=255)

                                   aCCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATcaac                                                      >  1:1882703/1‑93 (MQ=255)

                                   aCCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATcaac                                                      >  2:1744303/1‑93 (MQ=255)

                                     cGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATcaacaa                                                    <  2:1477821/93‑1 (MQ=255)

                                      gTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAg                                                   >  2:4359358/1‑93 (MQ=255)

                                      gTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAg                                                   >  2:1628277/1‑93 (MQ=255)

                                       ttGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGc                                                  <  2:3553179/93‑1 (MQ=255)

                                        tGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGcc                                                 >  1:401399/1‑93 (MQ=255)

                                          aaGCCGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCgg                                               <  2:4153594/93‑1 (MQ=255)

                                           aGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGa                                              <  1:2644371/93‑1 (MQ=255)

                                           aGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGa                                              >  1:4428232/1‑93 (MQ=255)

                                                gTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTg                                         >  1:1644532/1‑93 (MQ=255)

                                                gTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGCATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTg                                         >  1:3042862/1‑93 (MQ=255)

                                                 tACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTgg                                        <  1:4232848/93‑1 (MQ=255)

                                                 tACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTgg                                        <  2:1104206/93‑1 (MQ=255)

                                                 gaCCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTgg                                        <  1:3518024/92‑1 (MQ=255)

                                                   ccGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTgccc                                      >  1:1011493/1‑90 (MQ=255)

                                                    cGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGgcgc                                     <  1:884044/93‑1 (MQ=255)

                                                      gTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAg                                   >  1:4166645/1‑93 (MQ=255)

                                                      gTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAg                                   >  2:46372/1‑93 (MQ=255)

                                                         tGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAg                                <  2:2072189/93‑1 (MQ=255)

                                                         tGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAg                                <  2:2542061/93‑1 (MQ=255)

                                                            aGGACGAAGTGGACGTGGTTGAAGGTATGCGGTTCGGCCGTGGCTCCCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAAc                             <  2:938637/93‑1 (MQ=255)

                                                            aGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAAc                             <  2:1791353/93‑1 (MQ=255)

                                                               aCGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTgg                          <  1:1194226/93‑1 (MQ=255)

                                                               aCGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTgg                          >  1:111472/1‑93 (MQ=255)

                                                                cGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGa                         <  1:3146218/93‑1 (MQ=255)

                                                                cGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGa                         <  1:3805036/93‑1 (MQ=255)

                                                                 gAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGaa                        <  1:2820342/93‑1 (MQ=255)

                                                                  aaCTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGaaa                       <  1:1707206/93‑1 (MQ=255)

                                                                   aCTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAg                      >  2:4072144/1‑93 (MQ=255)

                                                                   aCTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAg                      >  2:2223839/1‑93 (MQ=255)

                                                                   aCTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAg                      >  2:3372886/1‑93 (MQ=255)

                                                                    cTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGc                     >  2:3457050/1‑93 (MQ=255)

                                                                     tGGACGTTTTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAACCGGAAACTGGCGCAGTAGAACTGGAAAGcc                    <  2:3756707/93‑1 (MQ=255)

                                                                     tGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGcc                    <  2:2720462/93‑1 (MQ=255)

                                                                     tGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGcc                    <  2:1362479/93‑1 (MQ=255)

                                                                      ggACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGccc                   <  2:2123587/93‑1 (MQ=255)

                                                                       gACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCg                  >  1:1957375/1‑93 (MQ=255)

                                                                        aCGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGt                 <  2:505617/93‑1 (MQ=255)

                                                                         cGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGtt                <  1:452890/93‑1 (MQ=255)

                                                                          gTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTc               <  2:1236503/93‑1 (MQ=255)

                                                                             gTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATc            >  2:2922285/1‑93 (MQ=255)

                                                                             gTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATc            <  1:3901464/93‑1 (MQ=255)

                                                                              ttGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATcc           <  1:3639157/93‑1 (MQ=255)

                                                                              ttGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATcc           <  1:4146676/93‑1 (MQ=255)

                                                                               tGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCct          <  1:1625136/93‑1 (MQ=255)

                                                                                gAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCctg         >  1:1518543/1‑93 (MQ=255)

                                                                                 aaGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCctgc        <  1:2483790/93‑1 (MQ=255)

                                                                                 aaGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCctgc        >  2:1980271/1‑93 (MQ=255)

                                                                                  aGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCctgct       <  1:3840671/93‑1 (MQ=255)

                                                                                   ggTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCctgctg      >  1:1471436/1‑93 (MQ=255)

                                                                                   ggTATGCGATTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGACCGTTCATCctgctg      >  1:4480452/1‑93 (MQ=255)

                                                                                    gTATGCTGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTgctgg     <  2:3038070/93‑1 (MQ=255)

                                                                                     tATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTgctggc    <  1:1318181/93‑1 (MQ=255)

                                                                                     tATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTgctggc    >  2:1025032/1‑93 (MQ=255)

                                                                                     tATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTgctggc    >  1:758777/1‑93 (MQ=255)

                                                                                       tGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTgctggctg  >  1:3204595/1‑93 (MQ=255)

AAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTG  >  NC_000913/4369093‑4369272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACAA  >  NC_000913/4369090‑4369276

CTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCG                                                                                               <  SRR4302535.3630478/94‑1 (MQ=60)

CTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCG                                                                                               >  SRR4302535.3821848/1‑94 (MQ=60)

CTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCG                                                                                               >  SRR4302535.3918233/1‑94 (MQ=60)

CTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCG                                                                                               >  SRR4302535.496692/1‑94 (MQ=60)

 TGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGG                                                                                              >  SRR4302535.4514536/1‑94 (MQ=60)

     GCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCG                                                                                          <  SRR4302535.917280/94‑1 (MQ=60)

      CGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGA                                                                                         >  SRR4302535.2253335/1‑94 (MQ=60)

      CGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGA                                                                                         <  SRR4302535.2455641/94‑1 (MQ=60)

       GATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGAC                                                                                        >  SRR4302535.1473235/1‑94 (MQ=60)

           GACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTG                                                                                    <  SRR4302535.3033158/94‑1 (MQ=60)

           GACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTG                                                                                    >  SRR4302535.579637/1‑94 (MQ=60)

             CAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGC                                                                                  >  SRR4302535.1397761/1‑94 (MQ=60)

              AAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCT                                                                                 >  SRR4302535.3182461/1‑94 (MQ=60)

               AAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTA                                                                                <  SRR4302535.2580760/94‑1 (MQ=60)

               AAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTA                                                                                >  SRR4302535.2319339/1‑94 (MQ=60)

               AAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTA                                                                                >  SRR4302535.811285/1‑94 (MQ=60)

                 GTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACC                                                                              <  SRR4302535.4096939/94‑1 (MQ=60)

                 GTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGGACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACA                                                                              >  SRR4302535.1836305/1‑94 (MQ=60)

                  TCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCT                                                                             <  SRR4302535.45424/94‑1 (MQ=60)

                    GGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGT                                                                           >  SRR4302535.3178514/1‑94 (MQ=60)

                     GTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTC                                                                          >  SRR4302535.3523987/1‑94 (MQ=60)

                      TAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCT                                                                         <  SRR4302535.2251993/94‑1 (MQ=60)

                       AAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTC                                                                        >  SRR4302535.4312144/1‑94 (MQ=60)

                       AAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTC                                                                        <  SRR4302535.4366367/94‑1 (MQ=60)

                        AAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCC                                                                       >  SRR4302535.3954364/1‑94 (MQ=60)

                         AGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCT                                                                      >  SRR4302535.4333488/1‑94 (MQ=60)

                         AGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCT                                                                      >  SRR4302535.538021/1‑94 (MQ=60)

                            AGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTAC                                                                   >  SRR4302535.2484205/1‑94 (MQ=60)

                            AGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTAC                                                                   >  SRR4302535.880121/1‑94 (MQ=60)

                            AGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTAC                                                                   >  SRR4302535.3481226/1‑94 (MQ=60)

                            AGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTAC                                                                   >  SRR4302535.1634576/1‑94 (MQ=60)

                               CGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTC                                                                >  SRR4302535.515578/1‑94 (MQ=60)

                               CGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTC                                                                <  SRR4302535.1797140/94‑1 (MQ=60)

                               CGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCCACCGTGGCTACCTGTCTCCTTACTTC                                                                >  SRR4302535.2051138/1‑94 (MQ=60)

                                 TTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCAT                                                              >  SRR4302535.1497328/1‑94 (MQ=60)

                                 TTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCAT                                                              <  SRR4302535.1668408/94‑1 (MQ=60)

                                  TATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATC                                                             >  SRR4302535.3691975/1‑94 (MQ=60)

                                    TCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAA                                                           <  SRR4302535.1162226/94‑1 (MQ=60)

                                    TCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAA                                                           >  SRR4302535.1620346/1‑94 (MQ=60)

                                     CACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAAC                                                          >  SRR4302535.2392375/1‑94 (MQ=60)

                                     CACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAAC                                                          >  SRR4302535.1896271/1‑94 (MQ=60)

                                      CCCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACA                                                         <  SRR4302535.1088704/94‑1 (MQ=60)

                                      ACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACA                                                         <  SRR4302535.3905573/94‑1 (MQ=60)

                                          TTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCC                                                     <  SRR4302535.1006372/94‑1 (MQ=60)

                                          TTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCC                                                     >  SRR4302535.404212/1‑94 (MQ=60)

                                             AAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGA                                                  >  SRR4302535.4458567/1‑94 (MQ=60)

                                             AAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGA                                                  <  SRR4302535.1941766/94‑1 (MQ=60)

                                              AGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAA                                                 >  SRR4302535.1582611/1‑94 (MQ=60)

                                              AGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAA                                                 <  SRR4302535.2663309/94‑1 (MQ=60)

                                                ACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAAC                                               <  SRR4302535.4397232/94‑1 (MQ=60)

                                                 CGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACT                                              <  SRR4302535.4184699/94‑1 (MQ=60)

                                                 CGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACT                                              <  SRR4302535.4502022/94‑1 (MQ=60)

                                                  GGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTG                                             <  SRR4302535.4227716/94‑1 (MQ=60)

                                                  GGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTG                                             >  SRR4302535.1656418/1‑94 (MQ=60)

                                                  GGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCGCCTTACTTCATCAACAAGCCGGAAACTG                                             >  SRR4302535.4541644/1‑94 (MQ=60)

                                                  GGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGCATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTG                                             >  SRR4302535.3064183/1‑94 (MQ=60)

                                                    TACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGC                                           <  SRR4302535.4261814/94‑1 (MQ=60)

                                                    TACCGGCCTGCAGGCCGACCTGGACGTGGTGGAAGGTATGCGGTTCGACCGTGGCTACGTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGC                                           <  SRR4302535.4325722/94‑1 (MQ=60)

                                                    GACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGC                                           <  SRR4302535.3542462/94‑1 (MQ=60)

                                                     ACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGccc                                          >  SRR4302535.1018678/1‑91 (MQ=60)

                                                     ACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCG                                          >  SRR4302535.4311195/1‑94 (MQ=60)

                                                      CCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGC                                         >  SRR4302535.1296741/1‑94 (MQ=60)

                                                       CGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCA                                        <  SRR4302535.890299/94‑1 (MQ=60)

                                                        GGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAG                                       >  SRR4302535.4195173/1‑94 (MQ=60)

                                                         GTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGT                                      >  SRR4302535.4147331/1‑94 (MQ=60)

                                                          TCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTA                                     <  SRR4302535.4264415/94‑1 (MQ=60)

                                                           CTGCAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAG                                    <  SRR4302535.3225849/94‑1 (MQ=60)

                                                              CAGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAAC                                 >  SRR4302535.3568709/1‑94 (MQ=60)

                                                               AGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACT                                <  SRR4302535.1973842/94‑1 (MQ=60)

                                                               AGGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACT                                >  SRR4302535.409264/1‑94 (MQ=60)

                                                                GGACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTG                               >  SRR4302535.2670009/1‑94 (MQ=60)

                                                                 GACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGG                              >  SRR4302535.112257/1‑94 (MQ=60)

                                                                  ACGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGA                             <  SRR4302535.1202781/94‑1 (MQ=60)

                                                                   CGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAA                            <  SRR4302535.3831263/94‑1 (MQ=60)

                                                                   CGAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAA                            <  SRR4302535.3168096/94‑1 (MQ=60)

                                                                    GAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAA                           >  SRR4302535.2183913/1‑94 (MQ=60)

                                                                    GAACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAA                           <  SRR4302535.2840373/94‑1 (MQ=60)

                                                                     AACTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAG                          <  SRR4302535.1719549/94‑1 (MQ=60)

                                                                       CTGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTNGCGCAGTAGAACTGGAAAGCC                        >  SRR4302535.1649934/1‑94 (MQ=60)

                                                                        TGGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCC                       >  SRR4302535.1138449/1‑94 (MQ=60)

                                                                         GGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCG                      >  SRR4302535.1971464/1‑94 (MQ=60)

                                                                         GGACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCG                      <  SRR4302535.4026676/94‑1 (MQ=60)

                                                                          GACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGCAGAACTGGAAAGCCCGT                     >  SRR4302535.1792825/1‑94 (MQ=60)

                                                                           ACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTT                    <  SRR4302535.549068/94‑1 (MQ=60)

                                                                           ACGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTT                    <  SRR4302535.225616/94‑1 (MQ=60)

                                                                            CGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTC                   <  SRR4302535.456062/94‑1 (MQ=60)

                                                                            CGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTC                   <  SRR4302535.4600976/94‑1 (MQ=60)

                                                                            CGTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTC                   <  SRR4302535.2734052/94‑1 (MQ=60)

                                                                             GTGGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCA                  >  SRR4302535.7755/1‑94 (MQ=60)

                                                                             GTAGTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCA                  <  SRR4302535.3103750/94‑1 (MQ=60)

                                                                                GTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCC               <  SRR4302535.670579/94‑1 (MQ=60)

                                                                                GTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCC               <  SRR4302535.3928188/94‑1 (MQ=60)

                                                                                GTTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCC               <  SRR4302535.1666587/94‑1 (MQ=60)

                                                                                 TTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCT              <  SRR4302535.4175071/94‑1 (MQ=60)

                                                                                 TTGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCT              <  SRR4302535.3664369/94‑1 (MQ=60)

                                                                                  TGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTG             <  SRR4302535.1636869/94‑1 (MQ=60)

                                                                                  TGAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTG             >  SRR4302535.1529480/1‑94 (MQ=60)

                                                                                   GAAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGC            >  SRR4302535.3839726/1‑94 (MQ=60)

                                                                                    AAGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCT           <  SRR4302535.2501696/94‑1 (MQ=60)

                                                                                     AGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTG          <  SRR4302535.3867135/94‑1 (MQ=60)

                                                                                     AGGTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTG          >  SRR4302535.1481973/1‑94 (MQ=60)

                                                                                     AGGTATGCGATTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGACCGTTCATCCTGCTG          >  SRR4302535.4511207/1‑94 (MQ=60)

                                                                                       GTATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGC        >  SRR4302535.764122/1‑94 (MQ=60)

                                                                                        TATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCT       <  SRR4302535.1327598/94‑1 (MQ=60)

                                                                                         ATGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTG      >  SRR4302535.3226786/1‑94 (MQ=60)

                                                                                          TGCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGA     >  SRR4302535.3778867/1‑94 (MQ=60)

                                                                                           GCGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGAC    <  SRR4302535.104771/94‑1 (MQ=60)

                                                                                            CGGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACA   >  SRR4302535.3822639/1‑94 (MQ=60)

                                                                                             GGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACAA  >  SRR4302535.4332300/1‑94 (MQ=60)

CTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTGCAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACAA  >  NC_000913/4369090‑4369276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`