Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,372,457	A→G	V260A (GTG→GCG)	yjeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,372,457	0	A	G	100.0%	703.8 / NA	221	V260A (GTG→GCG)	yjeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (94/127); total (94/127)

Only 100 of 225 total aligned reads displayed.

ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546

 cTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCAt                                                                                        >  1:3888722/1‑93 (MQ=255)

   ttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCa                                                                                      <  2:61214/93‑1 (MQ=255)

    tATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAc                                                                                     >  1:706032/1‑93 (MQ=255)

    tATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAc                                                                                     <  2:4386482/93‑1 (MQ=255)

     aTCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACg                                                                                    >  1:2783375/1‑93 (MQ=255)

        gAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTaa                                                                                 <  1:3099242/93‑1 (MQ=255)

        gAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTaa                                                                                 <  2:3437680/93‑1 (MQ=255)

          gCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACa                                                                               >  1:279540/1‑93 (MQ=255)

          gCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACa                                                                               <  2:1670679/93‑1 (MQ=255)

          gCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACa                                                                               <  1:2385080/93‑1 (MQ=255)

           cacaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAg                                                                              <  1:1388536/93‑1 (MQ=255)

            acaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGa                                                                             <  2:3810312/93‑1 (MQ=255)

            acaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGa                                                                             >  1:167407/1‑93 (MQ=255)

             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  1:509757/93‑1 (MQ=255)

             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  1:3488332/93‑1 (MQ=255)

              aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGT‑ACAGAACg                                                                          >  2:1591957/1‑93 (MQ=255)

               tGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACg                                                                          <  1:4486952/93‑1 (MQ=255)

               tGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACg                                                                          <  1:2468140/93‑1 (MQ=255)

               tGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACg                                                                          >  2:1051904/1‑93 (MQ=255)

               tGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACg                                                                          >  2:4466312/1‑93 (MQ=255)

                gCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGc                                                                         <  1:646336/93‑1 (MQ=255)

                gCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGc                                                                         >  1:1064429/1‑93 (MQ=255)

                 cAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGct                                                                        <  1:820754/93‑1 (MQ=255)

                  aGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctc                                                                       <  1:3718112/93‑1 (MQ=255)

                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctct                                                                      <  2:3073262/93‑1 (MQ=255)

                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     >  2:3979361/1‑93 (MQ=255)

                     tAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTgg                                                                    <  2:4581658/93‑1 (MQ=255)

                      aaTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGt                                                                   <  1:3689707/93‑1 (MQ=255)

                      aaTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGt                                                                   >  2:2255879/1‑93 (MQ=255)

                      aaTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGt                                                                   >  1:2224580/1‑93 (MQ=255)

                       aTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGtt                                                                  >  2:3189972/1‑93 (MQ=255)

                          cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAg                                                               >  2:2399319/1‑93 (MQ=255)

                           ggCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGc                                                              <  2:3704591/93‑1 (MQ=255)

                            gCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCa                                                             >  2:302048/1‑93 (MQ=255)

                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           <  2:3252093/93‑1 (MQ=255)

                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           >  1:1963604/1‑93 (MQ=255)

                               ttACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAg                                                          <  1:2690704/93‑1 (MQ=255)

                                 aCGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTa                                                        <  1:2643638/93‑1 (MQ=255)

                                 aCGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTa                                                        <  1:576301/93‑1 (MQ=255)

                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  1:2908079/93‑1 (MQ=255)

                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  2:4085623/93‑1 (MQ=255)

                                   gCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAc                                                      <  1:1347481/93‑1 (MQ=255)

                                   gCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAc                                                      <  1:419031/93‑1 (MQ=255)

                                      ggCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACTTAACAGAACGCTCTGGTTCAGCAAAGTAACGcc                                                   <  2:2429655/93‑1 (MQ=255)

                                      ggCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGcc                                                   >  2:2043116/1‑93 (MQ=255)

                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCt                                                  >  1:3022057/1‑93 (MQ=255)

                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGATCAGCAACGTAACGCCt                                                  >  1:1059386/1‑93 (MQ=255)

                                        cATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTa                                                 <  1:1015524/93‑1 (MQ=255)

                                        cATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTa                                                 <  2:1891788/93‑1 (MQ=255)

                                         aTCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAc                                                <  1:4101855/93‑1 (MQ=255)

                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  2:4680417/1‑93 (MQ=255)

                                                  cGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCaa                                       <  2:4628278/93‑1 (MQ=255)

                                                  cGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCaa                                       >  2:4179638/1‑93 (MQ=255)

                                                   gCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAc                                      <  2:2988867/93‑1 (MQ=255)

                                                     aTTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACtt                                    >  1:2021748/1‑93 (MQ=255)

                                                     aTTACTCAGGTTTGCCAGCGTTTGTGCGTGATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACtt                                    <  1:2122226/93‑1 (MQ=255)

                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  1:4011326/1‑93 (MQ=255)

                                                        aCTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGc                                 <  1:1199459/93‑1 (MQ=255)

                                                        aCTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGc                                 >  2:76295/1‑93 (MQ=255)

                                                         cTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGcc                                <  2:263259/93‑1 (MQ=255)

                                                          tCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCa                               >  1:3761855/1‑93 (MQ=255)

                                                          tCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCa                               <  1:296942/93‑1 (MQ=255)

                                                           cAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:927518/93‑1 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             <  2:1628200/93‑1 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  1:1900935/1‑93 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  2:4536714/1‑93 (MQ=255)

                                                             ggTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCg                            >  1:4396307/1‑93 (MQ=255)

                                                             ggTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCg                            >  2:2730182/1‑93 (MQ=255)

                                                             ggTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAACCGGCGCAACTTAGCCATCg                            >  2:1878849/1‑93 (MQ=255)

                                                               tttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGcc                          >  2:3743418/1‑93 (MQ=255)

                                                               tttGCCAGCGTTGTTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGcc                          <  1:3328302/93‑1 (MQ=255)

                                                                ttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCt                         <  2:807566/93‑1 (MQ=255)

                                                                  gCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGa                       >  2:1063148/1‑93 (MQ=255)

                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      >  1:2075577/1‑93 (MQ=255)

                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     >  2:3160770/1‑93 (MQ=255)

                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     <  1:4443501/93‑1 (MQ=255)

                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     >  2:4194119/1‑93 (MQ=255)

                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     >  1:1990612/1‑93 (MQ=255)

                                                                      gCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGa                   <  1:1140318/93‑1 (MQ=255)

                                                                       cGTTTGTGCGTTATGGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGaa                  <  2:503858/93‑1 (MQ=255)

                                                                       cGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGaa                  <  2:2805767/93‑1 (MQ=255)

                                                                        gTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAt                 <  2:285801/93‑1 (MQ=255)

                                                                        gTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAt                 <  1:1907777/93‑1 (MQ=255)

                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                >  1:478445/1‑93 (MQ=255)

                                                                          ttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               <  2:2671425/93‑1 (MQ=255)

                                                                          ttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               >  1:61033/1‑93 (MQ=255)

                                                                           tgtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGtt              <  1:1580364/93‑1 (MQ=255)

                                                                            gtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGttt             <  1:319378/93‑1 (MQ=255)

                                                                             tgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTc            <  2:1464114/93‑1 (MQ=255)

                                                                                gTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATc         >  2:1207189/1‑93 (MQ=255)

                                                                                 ttATCGCTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCctct        >  2:3392498/1‑93 (MQ=255)

                                                                                   aTCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAc      >  1:4648212/1‑93 (MQ=255)

                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     >  2:3943004/1‑93 (MQ=255)

                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  2:2199014/93‑1 (MQ=255)

                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  1:3646970/93‑1 (MQ=255)

                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     >  1:3541242/1‑93 (MQ=255)

                                                                                     cGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCt    <  1:2600536/93‑1 (MQ=255)

                                                                                       ttCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCa  >  2:4047602/1‑93 (MQ=255)

                                                                                       ttCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCa  <  2:1805554/93‑1 (MQ=255)

                                                                                       ttCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGCTTCATCTACCTCa  >  1:4293019/1‑93 (MQ=255)

ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  >  NC_000913/4372364‑4372550

TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCG                                                                                               >  SRR4302535.2918690/1‑94 (MQ=60)

TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCG                                                                                               <  SRR4302535.4357844/94‑1 (MQ=60)

TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCG                                                                                               >  SRR4302535.1308954/1‑94 (MQ=60)

  TACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCA                                                                                             >  SRR4302535.2405429/1‑94 (MQ=60)

   ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCAT                                                                                            >  SRR4302535.3915390/1‑94 (MQ=60)

     TTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCC                                                                                          >  SRR4302535.846788/1‑94 (MQ=60)

     TTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGGTCGCATCA                                                                                          >  SRR4302535.4497212/1‑94 (MQ=60)

      TTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAC                                                                                         >  SRR4302535.710991/1‑94 (MQ=60)

       TATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACG                                                                                        <  SRR4302535.2112949/94‑1 (MQ=60)

       TATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACG                                                                                        >  SRR4302535.2803181/1‑94 (MQ=60)

           GAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAAC                                                                                    <  SRR4302535.3120820/94‑1 (MQ=60)

            AGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACA                                                                                   >  SRR4302535.281490/1‑94 (MQ=60)

             GCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAG                                                                                  <  SRR4302535.2402215/94‑1 (MQ=60)

              CACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGA                                                                                 <  SRR4302535.1398447/94‑1 (MQ=60)

              CACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGA                                                                                 >  SRR4302535.168570/1‑94 (MQ=60)

                CATTCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302535.1199492/94‑1 (MQ=60)

                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302535.3512550/94‑1 (MQ=60)

                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302535.1552388/94‑1 (MQ=60)

                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302535.513321/94‑1 (MQ=60)

                 ATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACG                                                                              <  SRR4302535.46183/94‑1 (MQ=60)

                 ATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACG                                                                              >  SRR4302535.373708/1‑94 (MQ=60)

                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGC                                                                             <  SRR4302535.4517753/94‑1 (MQ=60)

                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGC                                                                             <  SRR4302535.2485935/94‑1 (MQ=60)

                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGC                                                                             >  SRR4302535.1072003/1‑94 (MQ=60)

                   GCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCT                                                                            <  SRR4302535.650845/94‑1 (MQ=60)

                    CAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTC                                                                           >  SRR4302535.3164304/1‑94 (MQ=60)

                    CAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTC                                                                           <  SRR4302535.826513/94‑1 (MQ=60)

                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          <  SRR4302535.4435289/94‑1 (MQ=60)

                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          <  SRR4302535.3743873/94‑1 (MQ=60)

                      GGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCCTCACGTAACAGAACGCTCTG                                                                         <  SRR4302535.872963/94‑1 (MQ=60)

                      GGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTG                                                                         <  SRR4302535.3778324/94‑1 (MQ=60)

                       GTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGG                                                                        <  SRR4302535.4298919/94‑1 (MQ=60)

                        TAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGT                                                                       <  SRR4302535.1593700/94‑1 (MQ=60)

                        TAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGT                                                                       >  SRR4302535.2240412/1‑94 (MQ=60)

                         AATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTT                                                                      <  SRR4302535.3715246/94‑1 (MQ=60)

                              GGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCA                                                                 >  SRR4302535.3456404/1‑94 (MQ=60)

                                CATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAA                                                               >  SRR4302535.4335249/1‑94 (MQ=60)

                                CATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAA                                                               >  SRR4302535.1977762/1‑94 (MQ=60)

                                CATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAA                                                               >  SRR4302535.644325/1‑94 (MQ=60)

                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              <  SRR4302535.2952048/94‑1 (MQ=60)

                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              <  SRR4302535.1189978/94‑1 (MQ=60)

                                  TTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGT                                                             <  SRR4302535.2709935/94‑1 (MQ=60)

                                   TACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTA                                                            <  SRR4302535.3108729/94‑1 (MQ=60)

                                   TACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTA                                                            <  SRR4302535.1797340/94‑1 (MQ=60)

                                    ACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAA                                                           <  SRR4302535.993899/94‑1 (MQ=60)

                                    ACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAA                                                           <  SRR4302535.580350/94‑1 (MQ=60)

                                    ACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAA                                                           <  SRR4302535.2662572/94‑1 (MQ=60)

                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302535.2163189/94‑1 (MQ=60)

                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302535.1395504/94‑1 (MQ=60)

                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302535.1671909/94‑1 (MQ=60)

                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302535.2928634/94‑1 (MQ=60)

                                      GCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACG                                                         >  SRR4302535.1125905/1‑94 (MQ=60)

                                      GCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACG                                                         <  SRR4302535.1357100/94‑1 (MQ=60)

                                      GCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACG                                                         <  SRR4302535.421964/94‑1 (MQ=60)

                                       CCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGC                                                        >  SRR4302535.2824191/1‑94 (MQ=60)

                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      <  SRR4302535.2810317/94‑1 (MQ=60)

                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      >  SRR4302535.3043217/1‑94 (MQ=60)

                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGATCAGCAACGTAACGCCT                                                      >  SRR4302535.1066931/1‑94 (MQ=60)

                                          GCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTATCAGAACGCTCTGGTTCAGCAAAGTAACGCCTA                                                     <  SRR4302535.3554730/94‑1 (MQ=60)

                                          GCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTA                                                     <  SRR4302535.598795/94‑1 (MQ=60)

                                           CATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    <  SRR4302535.1022743/94‑1 (MQ=60)

                                           CATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    >  SRR4302535.4236101/1‑94 (MQ=60)

                                           CATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    >  SRR4302535.2223016/1‑94 (MQ=60)

                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   <  SRR4302535.4129938/94‑1 (MQ=60)

                                                 ACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCG                                              >  SRR4302535.595961/1‑94 (MQ=60)

                                                   AACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACCCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCA                                            >  SRR4302535.3202982/1‑94 (MQ=60)

                                                    ACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAA                                           <  SRR4302535.2644423/94‑1 (MQ=60)

                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          >  SRR4302535.2019888/1‑94 (MQ=60)

                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         >  SRR4302535.1915994/1‑94 (MQ=60)

                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         <  SRR4302535.2685690/94‑1 (MQ=60)

                                                       CATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTT                                        >  SRR4302535.2036322/1‑94 (MQ=60)

                                                        ATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTA                                       >  SRR4302535.4038768/1‑94 (MQ=60)

                                                        ATTACTCAGGTTTGCCAGCGTTTGTGCGTGATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTA                                       <  SRR4302535.2137528/94‑1 (MQ=60)

                                                         TTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAGCCGGCGCAACTTAG                                      >  SRR4302535.4101118/1‑94 (MQ=60)

                                                         TTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAG                                      >  SRR4302535.509509/1‑94 (MQ=60)

                                                         TTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAG                                      <  SRR4302535.3568599/94‑1 (MQ=60)

                                                         TTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAG                                      <  SRR4302535.537746/94‑1 (MQ=60)

                                                           ACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCC                                    <  SRR4302535.1208052/94‑1 (MQ=60)

                                                           ACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCC                                    >  SRR4302535.1948866/1‑94 (MQ=60)

                                                            CTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCA                                   >  SRR4302535.3787850/1‑94 (MQ=60)

                                                             TCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAT                                  <  SRR4302535.299012/94‑1 (MQ=60)

                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 >  SRR4302535.1914615/1‑94 (MQ=60)

                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 <  SRR4302535.934084/94‑1 (MQ=60)

                                                               AGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCG                                >  SRR4302535.4426390/1‑94 (MQ=60)

                                                                GGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGC                               >  SRR4302535.2376988/1‑94 (MQ=60)

                                                                GGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGC                               <  SRR4302535.3874338/94‑1 (MQ=60)

                                                                 GTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCC                              >  SRR4302535.2849072/1‑94 (MQ=60)

                                                                  TTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCT                             <  SRR4302535.3688459/94‑1 (MQ=60)

                                                                  TTTGCCAGCGTTGTTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCT                             <  SRR4302535.3351457/94‑1 (MQ=60)

                                                                     GCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAC                          >  SRR4302535.2090529/1‑94 (MQ=60)

                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         >  SRR4302535.70119/1‑94 (MQ=60)

                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         >  SRR4302535.2004946/1‑94 (MQ=60)

                                                                       CAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGCCGG                        >  SRR4302535.1290649/1‑94 (MQ=60)

                                                                       CAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGG                        <  SRR4302535.4473976/94‑1 (MQ=60)

                                                                         GCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAA                      <  SRR4302535.1148442/94‑1 (MQ=60)

                                                                          CGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAT                     <  SRR4302535.711443/94‑1 (MQ=60)

                                                                           GTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATG                    >  SRR4302535.481802/1‑94 (MQ=60)

                                                                           GTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATG                    <  SRR4302535.1921533/94‑1 (MQ=60)

                                                                            TTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGT                   >  SRR4302535.61490/1‑94 (MQ=60)

                                                                             TTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  >  SRR4302535.3303547/1‑94 (MQ=60)

                                                                             TTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  <  SRR4302535.4662445/94‑1 (MQ=60)

                                                                             TTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  <  SRR4302535.3633225/94‑1 (MQ=60)

                                                                              TGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTT                 <  SRR4302535.1591771/94‑1 (MQ=60)

                                                                              TGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTT                 <  SRR4302535.4202039/94‑1 (MQ=60)

                                                                              TGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTT                 <  SRR4302535.3036307/94‑1 (MQ=60)

                                                                               GTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTC                >  SRR4302535.1981907/1‑94 (MQ=60)

                                                                               GTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTC                <  SRR4302535.321586/94‑1 (MQ=60)

                                                                                 GCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAT              <  SRR4302535.1026645/94‑1 (MQ=60)

                                                                                 GCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAT              <  SRR4302535.3829240/94‑1 (MQ=60)

                                                                                 GCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAT              <  SRR4302535.1988498/94‑1 (MQ=60)

                                                                                   GTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCT            <  SRR4302535.2070841/94‑1 (MQ=60)

                                                                                    TTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTTATCTA           >  SRR4302535.2603709/1‑94 (MQ=60)

                                                                                     TATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAC          >  SRR4302535.4679696/1‑94 (MQ=60)

                                                                                     TATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAC          <  SRR4302535.4698127/94‑1 (MQ=60)

                                                                                      ATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACC         >  SRR4302535.3565848/1‑94 (MQ=60)

                                                                                       TCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCT        <  SRR4302535.3672222/94‑1 (MQ=60)

                                                                                        CGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTC       <  SRR4302535.2619204/94‑1 (MQ=60)

                                                                                         GTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA      >  SRR4302535.1070577/1‑94 (MQ=60)

                                                                                         GTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA      <  SRR4302535.2655213/94‑1 (MQ=60)

                                                                                         GTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA      <  SRR4302535.279719/94‑1 (MQ=60)

                                                                                         GTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGCTTCATCTACCTCA      >  SRR4302535.4322396/1‑94 (MQ=60)

                                                                                          TTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCAT     >  SRR4302535.4032728/1‑94 (MQ=60)

                                                                                          TTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCAT     <  SRR4302535.4004544/94‑1 (MQ=60)

                                                                                            CGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTG   <  SRR4302535.593667/94‑1 (MQ=60)

                                                                                            CGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTG   <  SRR4302535.3265757/94‑1 (MQ=60)

                                                                                             GCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  <  SRR4302535.2509445/94‑1 (MQ=60)

                                                                                             GCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  <  SRR4302535.3011344/94‑1 (MQ=60)

                                                                                             GCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  <  SRR4302535.195316/94‑1 (MQ=60)

TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  >  NC_000913/4372364‑4372550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`