Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,378,338 G→A A520V (GCG→GTG)  frdA ← FrdA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,378,3380GA98.5% 1140.3 / ‑2.0 334A520V (GCG→GTG) frdAFrdA
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base A (133/195);  total (134/200)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

Only 100 of 339 total aligned reads displayed.

TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGC  >  NC_000913/4378248‑4378427
                                                                                          |                                                                                         
 tGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCa                                                                                        <  1:1038953/93‑1 (MQ=255)
 tGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCa                                                                                        >  2:2001929/1‑93 (MQ=255)
 tGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCa                                                                                        <  1:2203533/93‑1 (MQ=255)
   aGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATa                                                                                      <  2:3510674/93‑1 (MQ=255)
    ggAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATAc                                                                                     <  1:4761979/93‑1 (MQ=255)
       aGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACAtt                                                                                  >  1:4012827/1‑93 (MQ=255)
        gTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTc                                                                                 >  1:541576/1‑93 (MQ=255)
        gTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTc                                                                                 <  1:2948384/93‑1 (MQ=255)
        gTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTc                                                                                 >  1:2660081/1‑93 (MQ=255)
         ttGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCa                                                                                <  1:7282601/93‑1 (MQ=255)
         ttGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCa                                                                                <  2:2494788/93‑1 (MQ=255)
          tGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAg                                                                               <  1:2727490/93‑1 (MQ=255)
           gACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGc                                                                              <  1:5453301/93‑1 (MQ=255)
              gtcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAc                                                                           <  1:3353739/93‑1 (MQ=255)
               tcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACg                                                                          >  2:7013385/1‑93 (MQ=255)
                 gtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGATTGCACCATACATTCAGCAACGtt                                                                        >  2:4335481/1‑93 (MQ=255)
                 gtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGtt                                                                        <  2:6288631/93‑1 (MQ=255)
                 gtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGtt                                                                        <  1:7382870/93‑1 (MQ=255)
                     cGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGa                                                                    >  2:6633364/1‑93 (MQ=255)
                     cGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGa                                                                    <  2:3685679/93‑1 (MQ=255)
                     cGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGa                                                                    <  2:7300898/93‑1 (MQ=255)
                      gCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAc                                                                   <  1:6516961/93‑1 (MQ=255)
                      gCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAc                                                                   <  2:5369854/93‑1 (MQ=255)
                       cTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAcc                                                                  <  2:1562986/93‑1 (MQ=255)
                        tCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCg                                                                 >  1:3113361/1‑93 (MQ=255)
                        tCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCg                                                                 >  1:5281057/1‑93 (MQ=255)
                         cGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGt                                                                <  1:3921733/93‑1 (MQ=255)
                         cGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGt                                                                <  2:6883539/93‑1 (MQ=255)
                          ggTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTg                                                               <  2:4997376/93‑1 (MQ=255)
                          ggTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAACGTg                                                               >  2:3962624/1‑93 (MQ=255)
                            tGCAACCTTCGTCCAGGCGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGc                                                             <  2:5053413/93‑1 (MQ=255)
                            tGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGc                                                             >  1:6370880/1‑93 (MQ=255)
                              cAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGccc                                                           >  2:4837007/1‑93 (MQ=255)
                                 ccTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGt                                                        >  2:7537905/1‑93 (MQ=255)
                                    tCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCa                                                     <  2:3011307/93‑1 (MQ=255)
                                     cGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                    <  1:191030/93‑1 (MQ=255)
                                     cGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                    <  1:421384/93‑1 (MQ=255)
                                     cGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                    <  2:4573720/93‑1 (MQ=255)
                                     cGTCCAGACGCTGGTGCGCCCCGCGGGACTCTTTACGAGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                    <  2:6345247/93‑1 (MQ=255)
                                       tCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATg                                                  >  1:914001/1‑93 (MQ=255)
                                       tCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATg                                                  >  2:6824189/1‑93 (MQ=255)
                                        ccAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATgg                                                 <  2:4629589/93‑1 (MQ=255)
                                        ccAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATgg                                                 <  1:1960898/93‑1 (MQ=255)
                                         cAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGgt                                                <  1:5796545/93‑1 (MQ=255)
                                           gACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGgtgt                                              >  2:3718254/1‑93 (MQ=255)
                                            aCGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTa                                             <  1:1775171/93‑1 (MQ=255)
                                             cGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTag                                            <  1:7203046/93‑1 (MQ=255)
                                              gCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTaga                                           <  2:2099262/93‑1 (MQ=255)
                                               cTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGGGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGGGTagag                                          >  1:1304931/1‑93 (MQ=255)
                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                        <  2:6682576/93‑1 (MQ=255)
                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                        <  2:2656140/93‑1 (MQ=255)
                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                        <  1:3538335/93‑1 (MQ=255)
                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                        <  2:1893147/93‑1 (MQ=255)
                                                  gTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAg                                       <  1:5369301/93‑1 (MQ=255)
                                                   tGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAgg                                      <  2:5508795/93‑1 (MQ=255)
                                                   tGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAgg                                      >  1:6697180/1‑93 (MQ=255)
                                                    gcgcgcCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGt                                     <  2:7890513/93‑1 (MQ=255)
                                                     cgcgcCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTc                                    <  2:3359243/93‑1 (MQ=255)
                                                       cgccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCgg                                  <  1:2273022/93‑1 (MQ=255)
                                                        gccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgt                                 <  1:6149774/93‑1 (MQ=255)
                                                         ccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtg                                >  1:763146/1‑93 (MQ=255)
                                                          cgcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtgt                               <  2:7862436/93‑1 (MQ=255)
                                                          cgcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtgt                               >  1:5680393/1‑93 (MQ=255)
                                                          cgcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtgt                               <  1:2697467/93‑1 (MQ=255)
                                                           gcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGtt                              >  1:4698533/1‑93 (MQ=255)
                                                           gcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGtt                              >  2:2309558/1‑93 (MQ=255)
                                                            cgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTg                             >  1:3323135/1‑93 (MQ=255)
                                                              ggACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGaa                           <  1:2446203/93‑1 (MQ=255)
                                                              ggACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGaa                           <  2:7672755/93‑1 (MQ=255)
                                                               gACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAac                          >  1:592568/1‑93 (MQ=255)
                                                               gACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAac                          <  1:5617928/93‑1 (MQ=255)
                                                                aCTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAaca                         <  1:4939538/93‑1 (MQ=255)
                                                                    tttACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCt                     >  1:7911972/1‑93 (MQ=255)
                                                                    tttACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCt                     >  1:5524697/1‑93 (MQ=255)
                                                                     ttACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTg                    >  2:6457777/1‑93 (MQ=255)
                                                                        cGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGaa                 <  2:4209201/93‑1 (MQ=255)
                                                                        cGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGaa                 <  1:6916670/93‑1 (MQ=255)
                                                                         gTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAg                >  1:5828830/1‑93 (MQ=255)
                                                                          tGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgt               <  1:3664846/93‑1 (MQ=255)
                                                                          tGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgt               <  1:5088512/93‑1 (MQ=255)
                                                                            ccATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgtgt             <  1:1123001/93‑1 (MQ=255)
                                                                            ccATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgtgt             <  2:2784312/93‑1 (MQ=255)
                                                                            ccATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgtgt             >  1:6847849/1‑93 (MQ=255)
                                                                             cATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTc            >  2:4246524/1‑93 (MQ=255)
                                                                              aTTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCg           <  1:4610012/93‑1 (MQ=255)
                                                                               ttGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCgg          >  1:6754349/1‑93 (MQ=255)
                                                                               ttGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCgg          >  1:2428590/1‑93 (MQ=255)
                                                                                 gCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTg        <  1:2046301/93‑1 (MQ=255)
                                                                                  cGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACCCGCTGGAAGTGTCGGTGa       <  2:411018/93‑1 (MQ=255)
                                                                                  cGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGa       <  2:5732670/93‑1 (MQ=255)
                                                                                   ggAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAt      >  1:3723271/1‑93 (MQ=255)
                                                                                   ggAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAt      >  2:5895736/1‑93 (MQ=255)
                                                                                   ggAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAt      >  1:7671162/1‑93 (MQ=255)
                                                                                   ggAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAt      >  1:1696742/1‑93 (MQ=255)
                                                                                    taGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATg     >  2:1267440/2‑93 (MQ=255)
                                                                                    gAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATg     >  2:2890291/1‑93 (MQ=255)
                                                                                    gAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATg     <  2:6101426/93‑1 (MQ=255)
                                                                                     aGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATgc    >  1:1463585/1‑93 (MQ=255)
                                                                                     aGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATgc    >  1:2909718/1‑93 (MQ=255)
                                                                                      gTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGATCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATgcg   <  2:1155491/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGC  >  NC_000913/4378248‑4378427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCACGC  >  NC_000913/4378245‑4378431
                                                                                             |                                                                                             
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCA                                                                                               <  SRR4302543.356932/94‑1 (MQ=60)
   TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCA                                                                                            >  SRR4302543.6296410/1‑94 (MQ=60)
   TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCA                                                                                            <  SRR4302543.6174233/94‑1 (MQ=60)
   TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCA                                                                                            <  SRR4302543.6956654/94‑1 (MQ=60)
    TGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCAT                                                                                           <  SRR4302543.1048199/94‑1 (MQ=60)
    TGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCAT                                                                                           <  SRR4302543.2223364/94‑1 (MQ=60)
    TGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCAT                                                                                           >  SRR4302543.3366989/1‑94 (MQ=60)
     GAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATA                                                                                          >  SRR4302543.5156894/1‑94 (MQ=60)
     GAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATA                                                                                          <  SRR4302543.464845/94‑1 (MQ=60)
      AGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATAC                                                                                         <  SRR4302543.4784679/94‑1 (MQ=60)
       GGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACA                                                                                        >  SRR4302543.5598858/1‑94 (MQ=60)
       GGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACA                                                                                        <  SRR4302543.5449496/94‑1 (MQ=60)
       GGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACA                                                                                        <  SRR4302543.4805491/94‑1 (MQ=60)
       GGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACA                                                                                        <  SRR4302543.4668389/94‑1 (MQ=60)
         AAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATT                                                                                      >  SRR4302543.4048663/1‑94 (MQ=60)
          AGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTC                                                                                     >  SRR4302543.2684198/1‑94 (MQ=60)
          AGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTC                                                                                     >  SRR4302543.546586/1‑94 (MQ=60)
           GTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCA                                                                                    <  SRR4302543.2975152/94‑1 (MQ=60)
            TTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAG                                                                                   <  SRR4302543.7352927/94‑1 (MQ=60)
             TGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGC                                                                                  >  SRR4302543.4237356/1‑94 (MQ=60)
             TGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGC                                                                                  <  SRR4302543.2752308/94‑1 (MQ=60)
              GACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCA                                                                                 <  SRR4302543.5502747/94‑1 (MQ=60)
               ACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAA                                                                                >  SRR4302543.111612/1‑94 (MQ=60)
               ACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAA                                                                                <  SRR4302543.7210633/94‑1 (MQ=60)
                CGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAC                                                                               <  SRR4302543.4000327/94‑1 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTTGTGCGCGCCGCGGGACTCTTTACGCGTCATTGCGGAGTGCACCATACATTCAGCACCG                                                                              >  SRR4302543.6377170/1‑94 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                              <  SRR4302543.3383925/94‑1 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                              <  SRR4302543.3432159/94‑1 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                              <  SRR4302543.2953531/94‑1 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                              >  SRR4302543.7329003/1‑94 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                              <  SRR4302543.4068318/94‑1 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                              >  SRR4302543.7688542/1‑94 (MQ=60)
                   CGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTT                                                                            >  SRR4302543.1912758/1‑94 (MQ=60)
                   CGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTT                                                                            <  SRR4302543.1903073/94‑1 (MQ=60)
                    GTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTC                                                                           <  SRR4302543.7453825/94‑1 (MQ=60)
                      CACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAG                                                                         >  SRR4302543.4747064/1‑94 (MQ=60)
                      CACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAG                                                                         <  SRR4302543.3601674/94‑1 (MQ=60)
                      CACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAG                                                                         <  SRR4302543.2468573/94‑1 (MQ=60)
                      CACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAG                                                                         <  SRR4302543.878985/94‑1 (MQ=60)
                      CACGCTCGGTGCAACCTTCGTCCAGACGCTGGGGCGCGCCGCGGGACTCTTTTAGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAG                                                                         >  SRR4302543.175050/1‑94 (MQ=60)
                       ACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGA                                                                        >  SRR4302543.2234943/1‑94 (MQ=60)
                        CGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAC                                                                       >  SRR4302543.5717718/1‑94 (MQ=60)
                        CGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAC                                                                       <  SRR4302543.5807014/94‑1 (MQ=60)
                         GCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGAGTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACC                                                                      <  SRR4302543.1308449/94‑1 (MQ=60)
                         GCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACC                                                                      <  SRR4302543.7723795/94‑1 (MQ=60)
                         GCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACC                                                                      <  SRR4302543.6578337/94‑1 (MQ=60)
                          CTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCG                                                                     >  SRR4302543.5328715/1‑94 (MQ=60)
                          CTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCG                                                                     >  SRR4302543.3141367/1‑94 (MQ=60)
                          CTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCG                                                                     <  SRR4302543.3387700/94‑1 (MQ=60)
                          CTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCG                                                                     >  SRR4302543.7440696/1‑94 (MQ=60)
                          CTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCG                                                                     <  SRR4302543.5434093/94‑1 (MQ=60)
                          CTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCG                                                                     <  SRR4302543.5082463/94‑1 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                   >  SRR4302543.5417743/1‑94 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                   <  SRR4302543.5257128/94‑1 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                   <  SRR4302543.5484686/94‑1 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                   <  SRR4302543.3956343/94‑1 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                   <  SRR4302543.6831323/94‑1 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                   >  SRR4302543.2142732/1‑94 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                   >  SRR4302543.6440973/1‑94 (MQ=60)
                             GGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGG                                                                  >  SRR4302543.6731210/1‑94 (MQ=60)
                             GGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGG                                                                  <  SRR4302543.1996287/94‑1 (MQ=60)
                             GGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGG                                                                  <  SRR4302543.2174676/94‑1 (MQ=60)
                              GTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGC                                                                 >  SRR4302543.6430802/1‑94 (MQ=60)
                              GTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGC                                                                 >  SRR4302543.6430801/1‑94 (MQ=60)
                                GCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCC                                                               <  SRR4302543.2750127/94‑1 (MQ=60)
                                 CAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCA                                                              <  SRR4302543.7512006/94‑1 (MQ=60)
                                 CAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCA                                                              >  SRR4302543.5464843/1‑94 (MQ=60)
                                  AACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAG                                                             >  SRR4302543.7805609/1‑94 (MQ=60)
                                  AACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAG                                                             <  SRR4302543.7917778/94‑1 (MQ=60)
                                    CCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTT                                                           <  SRR4302543.370665/94‑1 (MQ=60)
                                      TTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCA                                                         <  SRR4302543.2048665/94‑1 (MQ=60)
                                        CGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAAT                                                       <  SRR4302543.1494186/94‑1 (MQ=60)
                                        CGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAAT                                                       <  SRR4302543.495086/94‑1 (MQ=60)
                                        CGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAAT                                                       <  SRR4302543.192647/94‑1 (MQ=60)
                                        CGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAAT                                                       <  SRR4302543.192646/94‑1 (MQ=60)
                                        CGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAAT                                                       <  SRR4302543.425328/94‑1 (MQ=60)
                                         GTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATG                                                      >  SRR4302543.922022/1‑94 (MQ=60)
                                         GTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATG                                                      <  SRR4302543.268857/94‑1 (MQ=60)
                                         CTCCAGACGCTGTTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATG                                                      <  SRR4302543.2246073/94‑1 (MQ=60)
                                          TCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCTACGTTCAGACCGTGGCCCAGTTCAATGG                                                     >  SRR4302543.3897881/1‑94 (MQ=60)
                                          TCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGG                                                     <  SRR4302543.193558/94‑1 (MQ=60)
                                          TCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGG                                                     >  SRR4302543.7720779/1‑94 (MQ=60)
                                           CCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGT                                                    >  SRR4302543.2468089/1‑94 (MQ=60)
                                           CCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGT                                                    <  SRR4302543.4713344/94‑1 (MQ=60)
                                           CCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGT                                                    >  SRR4302543.3047152/1‑94 (MQ=60)
                                           CCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGT                                                    <  SRR4302543.1978488/94‑1 (MQ=60)
                                            CAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTG                                                   <  SRR4302543.5849278/94‑1 (MQ=60)
                                            CAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTG                                                   >  SRR4302543.3120743/1‑94 (MQ=60)
                                             CGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGT                                                  <  SRR4302543.1356890/94‑1 (MQ=60)
                                             AGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGT                                                  >  SRR4302543.2978401/1‑94 (MQ=60)
                                               ACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAG                                                <  SRR4302543.6776371/94‑1 (MQ=60)
                                               ACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAG                                                <  SRR4302543.1791033/94‑1 (MQ=60)
                                                CGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGA                                               <  SRR4302543.4057472/94‑1 (MQ=60)
                                                CGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGA                                               >  SRR4302543.2953066/1‑94 (MQ=60)
                                                CGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGA                                               <  SRR4302543.7272880/94‑1 (MQ=60)
                                                 NCNGGTGNGNGCCGCGGGACNNNNTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAG                                              <  SRR4302543.2232758/94‑1 (MQ=60)
                                                 GCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAG                                              <  SRR4302543.6828462/94‑1 (MQ=60)
                                                 GCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAACGGTGTAGAG                                              <  SRR4302543.5537354/94‑1 (MQ=60)
                                                 GCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGGGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGGGTAGAG                                              >  SRR4302543.1316519/1‑94 (MQ=60)
                                                  CTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGC                                             <  SRR4302543.2443385/94‑1 (MQ=60)
                                                  CTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGC                                             >  SRR4302543.342747/1‑94 (MQ=60)
                                                   TGGTGCGCGCCGCGGGCCTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCA                                            <  SRR4302543.5012494/94‑1 (MQ=60)
                                                   TGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCA                                            <  SRR4302543.8005263/94‑1 (MQ=60)
                                                   TGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCA                                            <  SRR4302543.1443530/94‑1 (MQ=60)
                                                    GGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAG                                           <  SRR4302543.7332209/94‑1 (MQ=60)
                                                    GGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAG                                           <  SRR4302543.67627/94‑1 (MQ=60)
                                                    GGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAG                                           <  SRR4302543.5067534/94‑1 (MQ=60)
                                                    GGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAG                                           <  SRR4302543.3569797/94‑1 (MQ=60)
                                                     GTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAGTGGTGTAGAGCAGG                                          >  SRR4302543.6466031/1‑94 (MQ=60)
                                                     GTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGG                                          >  SRR4302543.6760389/1‑94 (MQ=60)
                                                     GTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGG                                          <  SRR4302543.5417912/94‑1 (MQ=60)
                                                      TGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGT                                         <  SRR4302543.6921413/94‑1 (MQ=60)
                                                        CGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCATACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCG                                       <  SRR4302543.4639808/94‑1 (MQ=60)
                                                        CGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCG                                       >  SRR4302543.6126627/1‑94 (MQ=60)
                                                         GCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGG                                      >  SRR4302543.5764374/1‑94 (MQ=60)
                                                          CGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGT                                     <  SRR4302543.2293802/94‑1 (MQ=60)
                                                           GCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTG                                    <  SRR4302543.6208008/94‑1 (MQ=60)
                                                           GCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTG                                    >  SRR4302543.769872/1‑94 (MQ=60)
                                                            CCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGT                                   >  SRR4302543.5732242/1‑94 (MQ=60)
                                                            CCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGT                                   >  SRR4302543.5732241/1‑94 (MQ=60)
                                                             CGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGCCCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTT                                  <  SRR4302543.2721933/94‑1 (MQ=60)
                                                             CGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTT                                  >  SRR4302543.4741443/1‑94 (MQ=60)
                                                             CGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTT                                  <  SRR4302543.2721929/94‑1 (MQ=60)
                                                              GCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTG                                 >  SRR4302543.588014/1‑94 (MQ=60)
                                                              GCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTG                                 >  SRR4302543.5940039/1‑94 (MQ=60)
                                                              GCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTG                                 >  SRR4302543.3353112/1‑94 (MQ=60)
                                                               CGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGA                                >  SRR4302543.5413825/1‑94 (MQ=60)
                                                               CGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGA                                >  SRR4302543.3960658/1‑94 (MQ=60)
                                                                GGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAA                               <  SRR4302543.257765/94‑1 (MQ=60)
                                                                GGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAA                               >  SRR4302543.6524326/1‑94 (MQ=60)
                                                                 GGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAAC                              >  SRR4302543.7635323/1‑94 (MQ=60)
                                                                 GGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAAC                              <  SRR4302543.1979739/94‑1 (MQ=60)
                                                                 GGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAAC                              <  SRR4302543.2468253/94‑1 (MQ=60)
                                                                 GGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAAC                              >  SRR4302543.598009/1‑94 (MQ=60)
                                                                  GACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACA                             <  SRR4302543.5669122/94‑1 (MQ=60)
                                                                   ACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACAC                            >  SRR4302543.1671640/1‑94 (MQ=60)
                                                                   ACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACAC                            >  SRR4302543.2425082/1‑94 (MQ=60)
                                                                   ACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACAC                            >  SRR4302543.557336/1‑94 (MQ=60)
                                                                   ACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACAC                            >  SRR4302543.3378692/1‑94 (MQ=60)
                                                                   ACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACAC                            <  SRR4302543.4984516/94‑1 (MQ=60)
                                                                    CTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACG                           <  SRR4302543.548983/94‑1 (MQ=60)
                                                                    CTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACG                           >  SRR4302543.6988899/1‑94 (MQ=60)
                                                                    CTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACG                           <  SRR4302543.6677428/94‑1 (MQ=60)
                                                                     TCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGC                          >  SRR4302543.3793160/1‑94 (MQ=60)
                                                                     TCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGC                          >  SRR4302543.2379716/1‑94 (MQ=60)
                                                                      CTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCT                         >  SRR4302543.2464916/1‑94 (MQ=60)
                                                                      CTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCT                         >  SRR4302543.5942698/1‑94 (MQ=60)
                                                                      CTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCT                         >  SRR4302543.7986304/1‑94 (MQ=60)
                                                                      CTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCT                         <  SRR4302543.5876075/94‑1 (MQ=60)
                                                                      CTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCT                         >  SRR4302543.5574874/1‑94 (MQ=60)
                                                                      CTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCT                         >  SRR4302543.5698416/1‑94 (MQ=60)
                                                                       TTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTG                        <  SRR4302543.5593709/94‑1 (MQ=60)
                                                                        TTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGGTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGG                       <  SRR4302543.3797326/94‑1 (MQ=60)
                                                                           CGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAG                    <  SRR4302543.6981691/94‑1 (MQ=60)
                                                                           CGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAG                    <  SRR4302543.1110081/94‑1 (MQ=60)
                                                                           CGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAG                    >  SRR4302543.5881839/1‑94 (MQ=60)
                                                                            GTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGT                   <  SRR4302543.2690219/94‑1 (MQ=60)
                                                                            GTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGT                   <  SRR4302543.1299258/94‑1 (MQ=60)
                                                                             TGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTG                  <  SRR4302543.2777337/94‑1 (MQ=60)
                                                                             TGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTG                  <  SRR4302543.5134631/94‑1 (MQ=60)
                                                                             TGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTG                  <  SRR4302543.3697026/94‑1 (MQ=60)
                                                                              GCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGT                 >  SRR4302543.191269/1‑94 (MQ=60)
                                                                              GCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGT                 >  SRR4302543.6912457/1‑94 (MQ=60)
                                                                               GCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTC                <  SRR4302543.4784634/94‑1 (MQ=60)
                                                                               CCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTC                <  SRR4302543.741071/94‑1 (MQ=60)
                                                                               CCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTC                <  SRR4302543.1133190/94‑1 (MQ=60)
                                                                                 ATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGG              <  SRR4302543.4651889/94‑1 (MQ=60)
                                                                                 ATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGG              >  SRR4302543.6818138/1‑94 (MQ=60)
                                                                                 ATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGG              >  SRR4302543.2450506/1‑94 (MQ=60)
                                                                                   TGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTG            >  SRR4302543.1449265/1‑94 (MQ=60)
                                                                                   TGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTG            <  SRR4302543.3216077/94‑1 (MQ=60)
                                                                                    GCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGA           <  SRR4302543.2064643/94‑1 (MQ=60)
                                                                                     CGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          <  SRR4302543.5503147/94‑1 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGTTGAT          >  SRR4302543.3756011/1‑94 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          <  SRR4302543.7896213/94‑1 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          >  SRR4302543.1711960/1‑94 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          >  SRR4302543.7743851/1‑94 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          <  SRR4302543.926542/94‑1 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          <  SRR4302543.2799367/94‑1 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          >  SRR4302543.4840343/1‑94 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          >  SRR4302543.6839134/1‑94 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          <  SRR4302543.332215/94‑1 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT          >  SRR4302543.3756012/1‑94 (MQ=60)
                                                                                      GGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGCTCAATGGTGTAGAGCAGGTCGGTGTGGAACACGCGGGAAGAGTCGGggagg         >  SRR4302543.6079719/1‑89 (MQ=60)
                                                                                       GAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGC        >  SRR4302543.2936164/1‑94 (MQ=60)
                                                                                       GAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGC        >  SRR4302543.1476778/1‑94 (MQ=60)
                                                                                       GAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGCGTCGGTGATGC        >  SRR4302543.2893274/1‑94 (MQ=60)
                                                                                        AGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCG       <  SRR4302543.7049003/94‑1 (MQ=60)
                                                                                        AGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCG       <  SRR4302543.1756370/94‑1 (MQ=60)
                                                                                        AGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCG       <  SRR4302543.2590855/94‑1 (MQ=60)
                                                                                         GTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGC      >  SRR4302543.3594220/1‑94 (MQ=60)
                                                                                         GTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGCAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGgga      >  SRR4302543.2383493/1‑91 (MQ=60)
                                                                                          TGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCA     >  SRR4302543.1369039/1‑94 (MQ=60)
                                                                                            CACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGCAGTGTCGGTGATGCGCACG   >  SRR4302543.487200/1‑94 (MQ=60)
                                                                                             ACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCACGC  >  SRR4302543.7236589/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCACGC  >  NC_000913/4378245‑4378431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑