Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,378,338 G→A A520V (GCG→GTG)  frdA ← FrdA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,378,3380GA100.0% 636.0 / NA 180A520V (GCG→GTG) frdAFrdA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (78/102);  total (78/102)

Only 100 of 183 total aligned reads displayed.

TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGC  >  NC_000913/4378248‑4378425
                                                                                          |                                                                                       
ttGAGGAAGTTGACGTCGTCACGCTCGGTGCACCCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCAcc                                                                                       <  1:991471/93‑1 (MQ=255)
ttGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCAcc                                                                                       <  2:1880714/93‑1 (MQ=255)
  gAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCAt                                                                                     >  1:1438098/1‑93 (MQ=255)
   aGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATa                                                                                    <  1:510042/93‑1 (MQ=255)
   aGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATa                                                                                    <  2:4035820/93‑1 (MQ=255)
   aGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATa                                                                                    <  1:1966989/93‑1 (MQ=255)
    ggAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATAc                                                                                   >  1:1405438/1‑93 (MQ=255)
      aaGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACAt                                                                                 <  1:3030405/93‑1 (MQ=255)
       aGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACAtt                                                                                >  1:1286339/1‑93 (MQ=255)
         ttGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCa                                                                              >  2:3593996/1‑93 (MQ=255)
          tGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAg                                                                             <  1:1711244/93‑1 (MQ=255)
           gACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGc                                                                            <  2:4372404/93‑1 (MQ=255)
             cgtcgtCACGCTCGGTGCAACCTTCGTCCAGGCGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCaa                                                                          <  2:2934244/93‑1 (MQ=255)
             cgtcgtCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCaa                                                                          <  2:1988454/93‑1 (MQ=255)
             cgtcgtCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCaa                                                                          <  2:528006/93‑1 (MQ=255)
             cgtcgtCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCaa                                                                          >  1:1533688/1‑93 (MQ=255)
              gtcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAc                                                                         <  1:2621093/93‑1 (MQ=255)
              gtcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAc                                                                         <  1:4293911/93‑1 (MQ=255)
              gtcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAc                                                                         <  2:2866068/93‑1 (MQ=255)
              gtcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAc                                                                         <  2:2991409/93‑1 (MQ=255)
              gtcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAc                                                                         >  1:1468777/1‑93 (MQ=255)
                cgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGt                                                                       <  1:2984835/93‑1 (MQ=255)
                   cACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCa                                                                    <  1:4378486/93‑1 (MQ=255)
                      gCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGTAGTGCACCATACATTCAGCAACGTTCAGAc                                                                 <  2:760775/93‑1 (MQ=255)
                      gCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAc                                                                 <  2:1330618/93‑1 (MQ=255)
                      gCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAc                                                                 <  2:1189385/93‑1 (MQ=255)
                      ccTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAc                                                                 <  1:592322/92‑1 (MQ=255)
                       cTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAcc                                                                >  2:2553370/1‑93 (MQ=255)
                        tCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCg                                                               >  2:4608651/1‑93 (MQ=255)
                        tCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGAACCATACATTCAGCAACGTTCAGACCg                                                               >  2:715977/1‑93 (MQ=255)
                         cGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGt                                                              <  1:790256/93‑1 (MQ=255)
                           gTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTgg                                                            >  2:1866255/1‑93 (MQ=255)
                            tGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGc                                                           <  2:3896145/93‑1 (MQ=255)
                            tGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGc                                                           >  2:3894647/1‑93 (MQ=255)
                              cAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGccc                                                         <  1:4627821/93‑1 (MQ=255)
                              cAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGccc                                                         <  1:3402676/93‑1 (MQ=255)
                                 ccTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGt                                                      <  1:4177595/93‑1 (MQ=255)
                                 ccTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGt                                                      >  1:3483360/1‑93 (MQ=255)
                                  cTTCGTCCAGACGCTGGTGCGCGACGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGATCAGACCGTGGGCTAGtt                                                     >  2:1763740/1‑93 (MQ=255)
                                   ttCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAGCGTTCAGACCGTGGCCCAGTTc                                                    <  1:2401968/93‑1 (MQ=255)
                                   ttCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTc                                                    >  2:12599/1‑93 (MQ=255)
                                     tggccAGGCGCTGGTGCGCGGCGCGGGACTTTTTACGTGCCATTGCGGAGTTCCACATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                  <  2:354904/90‑1 (MQ=255)
                                      gTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAAt                                                 <  2:4566941/93‑1 (MQ=255)
                                       tCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCACTg                                                >  1:2150267/1‑93 (MQ=255)
                                       tCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATg                                                >  1:2816612/1‑93 (MQ=255)
                                       tCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATg                                                <  2:3370162/93‑1 (MQ=255)
                                         cAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGgt                                              <  2:536869/93‑1 (MQ=255)
                                           gACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGgtgt                                            <  2:573708/93‑1 (MQ=255)
                                            aCGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTa                                           <  1:33225/93‑1 (MQ=255)
                                             cGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTag                                          <  2:2313033/93‑1 (MQ=255)
                                             cGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTag                                          <  2:2268961/93‑1 (MQ=255)
                                             cGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTag                                          >  1:4316924/1‑93 (MQ=255)
                                              gcgggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTaga                                         <  1:115887/90‑1 (MQ=255)
                                              gCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTaga                                         <  1:1909456/93‑1 (MQ=255)
                                              gCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTaga                                         >  2:2656858/1‑93 (MQ=255)
                                              gCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTaga                                         <  2:4377724/93‑1 (MQ=255)
                                              gCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTaga                                         <  2:405937/93‑1 (MQ=255)
                                                tGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATGCATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGc                                       <  1:43487/93‑1 (MQ=255)
                                                tGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGc                                       >  2:3149833/1‑93 (MQ=255)
                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTagagaa                                      >  1:2374562/1‑91 (MQ=255)
                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                      >  2:33679/1‑93 (MQ=255)
                                                  gTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAg                                     <  1:4468597/93‑1 (MQ=255)
                                                    gcgcgcCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGt                                   <  1:1194310/93‑1 (MQ=255)
                                                    gcgcgcCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGt                                   <  2:2480587/93‑1 (MQ=255)
                                                    gcccgccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGt                                   <  2:2430830/90‑1 (MQ=255)
                                                     cgcgcCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTc                                  <  1:3706349/93‑1 (MQ=255)
                                                      gcgcCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCg                                 <  1:3231976/93‑1 (MQ=255)
                                                       cgccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCgg                                >  2:1934431/1‑93 (MQ=255)
                                                        gccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCggg                               >  2:3650467/1‑92 (MQ=255)
                                                        gccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgt                               <  2:1780971/93‑1 (MQ=255)
                                                        gccgcGCGACTCTTTACGTGCCATGGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgt                               <  2:64880/93‑1 (MQ=255)
                                                         ccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtg                              >  2:2800814/1‑93 (MQ=255)
                                                         ccgcGGGACTCTTTACGTGCCATTGAGGAGCGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGCGTAGAGCAGGTCGgtg                              >  1:231136/1‑93 (MQ=255)
                                                          cgcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtgt                             <  1:3423181/93‑1 (MQ=255)
                                                           gcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGtt                            >  1:4080953/1‑93 (MQ=255)
                                                            cgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTg                           >  2:4625815/1‑93 (MQ=255)
                                                            cgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTg                           >  1:1956081/1‑93 (MQ=255)
                                                             gggACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGa                          >  2:2265374/1‑93 (MQ=255)
                                                             gggACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGCTGa                          >  2:3212136/1‑93 (MQ=255)
                                                              ggACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGaa                         >  2:2762856/1‑93 (MQ=255)
                                                               gACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAac                        >  1:861192/1‑93 (MQ=255)
                                                                aCTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAaca                       >  2:1805477/1‑93 (MQ=255)
                                                                aCTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAaca                       >  2:1207230/1‑93 (MQ=255)
                                                                aCTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAaca                       >  2:3787224/1‑93 (MQ=255)
                                                                    tttACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCt                   >  1:3447200/1‑93 (MQ=255)
                                                                        cGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGaa               >  2:4470660/1‑93 (MQ=255)
                                                                         gTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAg              <  1:2482489/93‑1 (MQ=255)
                                                                          tGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgt             <  2:1596810/93‑1 (MQ=255)
                                                                          tGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgt             <  2:2925583/93‑1 (MQ=255)
                                                                          tGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgt             <  2:1705928/93‑1 (MQ=255)
                                                                           gCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGTTGTTGAACACGCTGGAAgtg            <  1:1085486/93‑1 (MQ=255)
                                                                            ccATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgtgt           >  2:4286980/1‑93 (MQ=255)
                                                                              aTTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCg         >  2:3014476/1‑93 (MQ=255)
                                                                               ttGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCgg        >  1:926112/1‑93 (MQ=255)
                                                                               ttGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCgg        >  1:2124853/1‑93 (MQ=255)
                                                                                tGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGt       <  1:277734/93‑1 (MQ=255)
                                                                                  cGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGa     <  2:3432286/93‑1 (MQ=255)
                                                                                  cGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGa     <  1:1001950/93‑1 (MQ=255)
                                                                                     aGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATgc  <  2:1960553/93‑1 (MQ=255)
                                                                                     aGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATgc  <  1:1680491/93‑1 (MQ=255)
                                                                                          |                                                                                       
TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGC  >  NC_000913/4378248‑4378425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCACG  >  NC_000913/4378245‑4378430
                                                                                             |                                                                                            
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCA                                                                                              >  SRR4302535.212312/1‑94 (MQ=60)
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCA                                                                                              >  SRR4302535.3586473/1‑94 (MQ=60)
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCA                                                                                              <  SRR4302535.3655243/94‑1 (MQ=60)
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCA                                                                                              <  SRR4302535.1778857/94‑1 (MQ=60)
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCA                                                                                              <  SRR4302535.4393430/94‑1 (MQ=60)
   TTGAGGAAGTTGACGTCGTCACGCTCGGTGCACCCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCA                                                                                           <  SRR4302535.998507/94‑1 (MQ=60)
    TGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCAT                                                                                          >  SRR4302535.1448378/1‑94 (MQ=60)
      AGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATAC                                                                                        <  SRR4302535.1981167/94‑1 (MQ=60)
      AGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATAC                                                                                        <  SRR4302535.513609/94‑1 (MQ=60)
      AGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATAC                                                                                        >  SRR4302535.1839956/1‑94 (MQ=60)
      AGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATAC                                                                                        >  SRR4302535.1415483/1‑94 (MQ=60)
        GAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACAT                                                                                      <  SRR4302535.4342876/94‑1 (MQ=60)
        GAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACAT                                                                                      <  SRR4302535.595660/94‑1 (MQ=60)
         AAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATT                                                                                     >  SRR4302535.2596506/1‑94 (MQ=60)
         AAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATT                                                                                     <  SRR4302535.3051620/94‑1 (MQ=60)
         AAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATT                                                                                     >  SRR4302535.1295506/1‑94 (MQ=60)
             TGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGC                                                                                 <  SRR4302535.1723613/94‑1 (MQ=60)
               ACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAA                                                                               >  SRR4302535.1544737/1‑94 (MQ=60)
               ACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAA                                                                               <  SRR4302535.4480167/94‑1 (MQ=60)
                CGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAC                                                                              >  SRR4302535.1479301/1‑94 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                             <  SRR4302535.4323291/94‑1 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                             <  SRR4302535.2639884/94‑1 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                             <  SRR4302535.3297335/94‑1 (MQ=60)
                 GTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACG                                                                             <  SRR4302535.2141914/94‑1 (MQ=60)
                   CGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTT                                                                           <  SRR4302535.3005772/94‑1 (MQ=60)
                    GTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTC                                                                          >  SRR4302535.4641004/1‑94 (MQ=60)
                    GTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTC                                                                          <  SRR4302535.3234357/94‑1 (MQ=60)
                      CACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAG                                                                        >  SRR4302535.1477611/1‑94 (MQ=60)
                      CACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAG                                                                        <  SRR4302535.4408445/94‑1 (MQ=60)
                       ACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGA                                                                       >  SRR4302535.901037/1‑94 (MQ=60)
                         CCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACC                                                                     <  SRR4302535.596473/94‑1 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                  <  SRR4302535.795799/94‑1 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                  <  SRR4302535.4195841/94‑1 (MQ=60)
                            CGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTG                                                                  <  SRR4302535.2887740/94‑1 (MQ=60)
                             GGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGG                                                                 >  SRR4302535.3403916/1‑94 (MQ=60)
                               TGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCC                                                               >  SRR4302535.3434222/1‑94 (MQ=60)
                                 CAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCA                                                             <  SRR4302535.3426355/94‑1 (MQ=60)
                                 CAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCA                                                             <  SRR4302535.249168/94‑1 (MQ=60)
                                 CAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCA                                                             <  SRR4302535.4659234/94‑1 (MQ=60)
                                  AACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAG                                                            >  SRR4302535.1718380/1‑94 (MQ=60)
                                   ACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGT                                                           >  SRR4302535.234541/1‑94 (MQ=60)
                                   ACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGT                                                           >  SRR4302535.3507547/1‑94 (MQ=60)
                                    CCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTT                                                          <  SRR4302535.4206192/94‑1 (MQ=60)
                                    CCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTT                                                          <  SRR4302535.2052077/94‑1 (MQ=60)
                                     CTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTC                                                         <  SRR4302535.1368845/94‑1 (MQ=60)
                                      TTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAGCGTTCAGACCGTGGCCCAGTTCA                                                        <  SRR4302535.2419245/94‑1 (MQ=60)
                                      TTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCA                                                        <  SRR4302535.2777795/94‑1 (MQ=60)
                                       TCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAA                                                       <  SRR4302535.4403404/94‑1 (MQ=60)
                                         GTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCACTG                                                     >  SRR4302535.2165730/1‑94 (MQ=60)
                                         GTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATG                                                     >  SRR4302535.2836619/1‑94 (MQ=60)
                                               ACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAG                                               <  SRR4302535.1971064/94‑1 (MQ=60)
                                               ACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAG                                               >  SRR4302535.4346455/1‑94 (MQ=60)
                                               ACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAG                                               <  SRR4302535.33480/94‑1 (MQ=60)
                                                CGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGA                                              >  SRR4302535.862732/1‑94 (MQ=60)
                                                 GCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAG                                             <  SRR4302535.1923221/94‑1 (MQ=60)
                                                 GCGGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAG                                             <  SRR4302535.116700/94‑1 (MQ=60)
                                                   TGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATGCATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCA                                           <  SRR4302535.43815/94‑1 (MQ=60)
                                                   TGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCG                                           >  SRR4302535.1264315/1‑94 (MQ=60)
                                                   TGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGAA                                           >  SRR4302535.2391619/1‑94 (MQ=60)
                                                     GTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGG                                         <  SRR4302535.4499259/94‑1 (MQ=60)
                                                       GCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTC                                       <  SRR4302535.1202865/94‑1 (MQ=60)
                                                        CGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCG                                      <  SRR4302535.3732014/94‑1 (MQ=60)
                                                         GCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGG                                     <  SRR4302535.3254388/94‑1 (MQ=60)
                                                           GCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTG                                   >  SRR4302535.1551082/1‑94 (MQ=60)
                                                           GCCGCGGGACTCTTTACGTGCCATTGAGGAGCGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGCGTAGAGCAGGTCGGTG                                   >  SRR4302535.232731/1‑94 (MQ=60)
                                                           GCCACGGGGCTCTTTACGTGCCNTTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGGGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTG                                   <  SRR4302535.4243131/94‑1 (MQ=60)
                                                            CCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGT                                  <  SRR4302535.1183648/94‑1 (MQ=60)
                                                            CCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGT                                  <  SRR4302535.3679992/94‑1 (MQ=60)
                                                             CGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTT                                 <  SRR4302535.3446987/94‑1 (MQ=60)
                                                             CGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTT                                 >  SRR4302535.4108866/1‑94 (MQ=60)
                                                              GCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTG                                >  SRR4302535.1970160/1‑94 (MQ=60)
                                                              GCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTG                                >  SRR4302535.2828066/1‑94 (MQ=60)
                                                                GGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAA                              >  SRR4302535.1538360/1‑94 (MQ=60)
                                                                 GGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAAC                             >  SRR4302535.867275/1‑94 (MQ=60)
                                                                 GGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAAC                             >  SRR4302535.2186899/1‑94 (MQ=60)
                                                                    CTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACG                          <  SRR4302535.1133736/94‑1 (MQ=60)
                                                                      CTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCT                        >  SRR4302535.3471170/1‑94 (MQ=60)
                                                                      CTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCT                        >  SRR4302535.4666394/1‑94 (MQ=60)
                                                                         TACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGA                     <  SRR4302535.935794/94‑1 (MQ=60)
                                                                            GTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGT                  <  SRR4302535.2500389/94‑1 (MQ=60)
                                                                             TGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTG                 >  SRR4302535.3793812/1‑94 (MQ=60)
                                                                              GCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGTTGTTGAACACGCTGGAAGTGT                <  SRR4302535.1093201/94‑1 (MQ=60)
                                                                                 ATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGG             >  SRR4302535.2140165/1‑94 (MQ=60)
                                                                                 ATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGG             >  SRR4302535.932673/1‑94 (MQ=60)
                                                                                   TGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTG           <  SRR4302535.279671/94‑1 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT         <  SRR4302535.1009068/94‑1 (MQ=60)
                                                                                     CGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAT         >  SRR4302535.3461851/1‑94 (MQ=60)
                                                                                        AGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCG      <  SRR4302535.1692663/94‑1 (MQ=60)
                                                                                          TGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCA    >  SRR4302535.2841204/1‑94 (MQ=60)
                                                                                          TGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCA    >  SRR4302535.2210083/1‑94 (MQ=60)
                                                                                            CACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCACG  <  SRR4302535.932736/94‑1 (MQ=60)
                                                                                            CACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCACG  >  SRR4302535.3679141/1‑94 (MQ=60)
                                                                                            CACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCACG  >  SRR4302535.1085879/1‑94 (MQ=60)
                                                                                             |                                                                                            
TGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGCACG  >  NC_000913/4378245‑4378430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑