Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,372,457 A→G V260A (GTG→GCG)  yjeK ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,372,4570AG99.5% 1270.6 / ‑4.5 402V260A (GTG→GCG) yjeKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (196/204);  total (197/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

Only 100 of 413 total aligned reads displayed.

ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546
                                                                                          |                                                                                         
  tttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATc                                                                                       <  2:2971682/93‑1 (MQ=255)
  tttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATc                                                                                       <  1:4306357/93‑1 (MQ=255)
  tttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATc                                                                                       <  2:1547815/93‑1 (MQ=255)
   ttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCa                                                                                      <  2:4770141/93‑1 (MQ=255)
       cGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTa                                                                                  <  1:2571262/93‑1 (MQ=255)
        gAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTaa                                                                                 >  2:2632435/1‑93 (MQ=255)
        gAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTaa                                                                                 >  1:2335597/1‑93 (MQ=255)
         aGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAAc                                                                                <  1:1409114/93‑1 (MQ=255)
          gCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACa                                                                               <  1:5668328/93‑1 (MQ=255)
           cacaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAg                                                                              <  1:7409783/93‑1 (MQ=255)
            acaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGa                                                                             >  1:378799/1‑93 (MQ=255)
             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  2:215643/93‑1 (MQ=255)
             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  2:782756/93‑1 (MQ=255)
             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  2:4081793/93‑1 (MQ=255)
              aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAc                                                                           <  2:7553027/93‑1 (MQ=255)
              aTGCAGGTAATACCGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAc                                                                           <  2:2578064/93‑1 (MQ=255)
               tGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACg                                                                          >  2:119276/1‑93 (MQ=255)
                gCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGc                                                                         >  1:7291989/1‑93 (MQ=255)
                  aGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctc                                                                       <  2:2582591/93‑1 (MQ=255)
                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctct                                                                      <  1:1003028/93‑1 (MQ=255)
                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     <  1:6622490/93‑1 (MQ=255)
                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     >  2:5522302/1‑93 (MQ=255)
                     tAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTgg                                                                    <  2:4532020/93‑1 (MQ=255)
                     tAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTgg                                                                    <  2:5930967/93‑1 (MQ=255)
                      aaTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGt                                                                   <  1:1841587/93‑1 (MQ=255)
                       aTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACATAACGCTCTGGtt                                                                  <  1:136746/93‑1 (MQ=255)
                       aTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGtt                                                                  <  1:2435265/93‑1 (MQ=255)
                        tACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTc                                                                 >  2:6406067/1‑93 (MQ=255)
                          cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAg                                                               >  1:4459546/1‑93 (MQ=255)
                           ggCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGc                                                              <  1:575441/93‑1 (MQ=255)
                           ggCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGc                                                              >  2:3844778/1‑93 (MQ=255)
                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           >  2:1042232/1‑93 (MQ=255)
                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           >  1:3696806/1‑93 (MQ=255)
                               ttACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAg                                                          >  2:1631931/1‑93 (MQ=255)
                                 tcggcGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTa                                                        <  1:803408/89‑1 (MQ=255)
                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  2:3288075/93‑1 (MQ=255)
                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  2:6111111/93‑1 (MQ=255)
                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  1:1918496/93‑1 (MQ=255)
                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  1:2901356/93‑1 (MQ=255)
                                   gCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAc                                                      <  1:359487/93‑1 (MQ=255)
                                     cGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGc                                                    <  2:6680577/93‑1 (MQ=255)
                                      ggCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGcc                                                   >  2:3745429/1‑93 (MQ=255)
                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCt                                                  >  1:6274990/1‑93 (MQ=255)
                                        cATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTa                                                 <  2:307007/93‑1 (MQ=255)
                                         aTCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAc                                                >  2:1862074/1‑93 (MQ=255)
                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  1:6524486/1‑93 (MQ=255)
                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  1:1741873/1‑93 (MQ=255)
                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  1:7798583/1‑93 (MQ=255)
                                            gAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCg                                             <  1:7058585/93‑1 (MQ=255)
                                            gAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCg                                             >  1:2755824/1‑93 (MQ=255)
                                            gAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCg                                             <  1:3015238/93‑1 (MQ=255)
                                                aacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGgcgc                                         >  1:534307/1‑93 (MQ=255)
                                                aacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGgcgc                                         >  1:1287787/1‑93 (MQ=255)
                                                 acGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCa                                        >  1:752328/1‑93 (MQ=255)
                                                  cGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCaa                                       >  2:837423/1‑93 (MQ=255)
                                                  cGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCaa                                       >  1:4091195/1‑93 (MQ=255)
                                                   gCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAc                                      >  1:4845624/1‑93 (MQ=255)
                                                   gCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAc                                      <  1:5061534/93‑1 (MQ=255)
                                                    cATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACt                                     >  2:7145479/1‑93 (MQ=255)
                                                     aTTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACtt                                    >  1:4588345/1‑93 (MQ=255)
                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  2:178806/1‑93 (MQ=255)
                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  2:3542012/1‑93 (MQ=255)
                                                          tCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCa                               <  1:685192/93‑1 (MQ=255)
                                                           cAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:2646045/93‑1 (MQ=255)
                                                           cAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:2116101/93‑1 (MQ=255)
                                                           cAGGGTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:4656805/93‑1 (MQ=255)
                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  1:7673836/1‑93 (MQ=255)
                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  2:7257631/1‑93 (MQ=255)
                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  2:7183401/1‑93 (MQ=255)
                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             <  2:5114813/93‑1 (MQ=255)
                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  2:1972732/1‑93 (MQ=255)
                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             <  1:5932343/93‑1 (MQ=255)
                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             <  1:7175707/93‑1 (MQ=255)
                                                              gTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGc                           >  2:4003482/1‑93 (MQ=255)
                                                               tttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGcc                          <  2:6150092/93‑1 (MQ=255)
                                                               tttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGcc                          >  2:2430591/1‑93 (MQ=255)
                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      >  1:3954998/1‑93 (MQ=255)
                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      <  2:4680369/93‑1 (MQ=255)
                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     >  1:6742983/1‑93 (MQ=255)
                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     >  1:6447444/1‑93 (MQ=255)
                                                                       cGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGaa                  <  1:4175195/93‑1 (MQ=255)
                                                                         tttGTGCGTTATCTTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                <  1:1578426/93‑1 (MQ=255)
                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                >  2:5386410/1‑93 (MQ=255)
                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                >  2:3113570/1‑93 (MQ=255)
                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                >  1:5485164/1‑93 (MQ=255)
                                                                          ttttgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               <  2:2722022/90‑1 (MQ=255)
                                                                          ttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               <  2:5306370/93‑1 (MQ=255)
                                                                           tgtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGtt              <  1:3877200/93‑1 (MQ=255)
                                                                             tgCGTTATCGTTCTCATCACGTAACAGACCGCTATGTTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTc            <  2:4468210/93‑1 (MQ=255)
                                                                              gCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCa           <  1:6179395/93‑1 (MQ=255)
                                                                                gTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATc         <  2:1665331/93‑1 (MQ=255)
                                                                                 ttATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCt        >  1:1124699/1‑93 (MQ=255)
                                                                                  tATCGTTCGCATCACGTAACAGACCGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       <  2:4292092/93‑1 (MQ=255)
                                                                                  tATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       >  2:4962576/1‑93 (MQ=255)
                                                                                  tATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       >  1:3328598/1‑93 (MQ=255)
                                                                                   aTCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAc      >  2:6980325/1‑93 (MQ=255)
                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     >  2:602591/1‑93 (MQ=255)
                                                                                    tCGTCCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  1:3099655/93‑1 (MQ=255)
                                                                                     cGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCt    >  1:3544938/1‑93 (MQ=255)
                                                                                      gTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTc   <  2:7236642/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 228 total aligned reads displayed.
TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  >  NC_000913/4372364‑4372550
                                                                                             |                                                                                             
TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCG                                                                                               <  SRR4302543.1327609/94‑1 (MQ=60)
TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCG                                                                                               >  SRR4302543.7980319/1‑94 (MQ=60)
 GTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGC                                                                                              >  SRR4302543.43781/1‑94 (MQ=60)
   ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGCTTGTGCGTTATCGTTCACAT                                                                                            >  SRR4302543.1836138/1‑94 (MQ=60)
    CTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATC                                                                                           >  SRR4302543.3019922/1‑94 (MQ=60)
     TTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCA                                                                                          <  SRR4302543.4345480/94‑1 (MQ=60)
      TTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAC                                                                                         >  SRR4302543.3973228/1‑94 (MQ=60)
       TATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACG                                                                                        <  SRR4302543.340598/94‑1 (MQ=60)
       TATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACG                                                                                        >  SRR4302543.2612916/1‑94 (MQ=60)
        ATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGT                                                                                       >  SRR4302543.3077611/1‑94 (MQ=60)
        ATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGT                                                                                       >  SRR4302543.1896472/1‑94 (MQ=60)
          CGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAA                                                                                     >  SRR4302543.2356789/1‑94 (MQ=60)
          CGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAA                                                                                     <  SRR4302543.2594715/94‑1 (MQ=60)
           GAGCACATGCAGGTAATACGGCATTACGCCGGCATCTAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAAC                                                                                    >  SRR4302543.1285572/1‑94 (MQ=60)
           GAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAAC                                                                                    <  SRR4302543.7467253/94‑1 (MQ=60)
            AGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACA                                                                                   <  SRR4302543.6942589/94‑1 (MQ=60)
            AGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACA                                                                                   <  SRR4302543.1421848/94‑1 (MQ=60)
             GCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAG                                                                                  >  SRR4302543.4466323/1‑94 (MQ=60)
             GCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAG                                                                                  <  SRR4302543.5720054/94‑1 (MQ=60)
              CACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGA                                                                                 <  SRR4302543.7480883/94‑1 (MQ=60)
              CACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGA                                                                                 >  SRR4302543.382290/1‑94 (MQ=60)
              CACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGA                                                                                 >  SRR4302543.3745345/1‑94 (MQ=60)
               ACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAA                                                                                <  SRR4302543.6441734/94‑1 (MQ=60)
               ACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAA                                                                                >  SRR4302543.73211/1‑94 (MQ=60)
               ACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAA                                                                                >  SRR4302543.5515468/1‑94 (MQ=60)
               ACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAA                                                                                >  SRR4302543.5664980/1‑94 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               >  SRR4302543.5743201/1‑94 (MQ=60)
                 ATGTAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACG                                                                              <  SRR4302543.2059467/94‑1 (MQ=60)
                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGC                                                                             >  SRR4302543.4168082/1‑94 (MQ=60)
                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGC                                                                             <  SRR4302543.5857498/94‑1 (MQ=60)
                   GCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCT                                                                            <  SRR4302543.1824807/94‑1 (MQ=60)
                   GCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCT                                                                            >  SRR4302543.7779115/1‑94 (MQ=60)
                   GCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCT                                                                            <  SRR4302543.4610807/94‑1 (MQ=60)
                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          <  SRR4302543.7428513/94‑1 (MQ=60)
                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          <  SRR4302543.1987702/94‑1 (MQ=60)
                      GGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTG                                                                         <  SRR4302543.1011894/94‑1 (MQ=60)
                      GGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTG                                                                         <  SRR4302543.5268782/94‑1 (MQ=60)
                      GGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTG                                                                         >  SRR4302543.7506526/1‑94 (MQ=60)
                       GTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGG                                                                        <  SRR4302543.2255312/94‑1 (MQ=60)
                       GTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGG                                                                        <  SRR4302543.2255313/94‑1 (MQ=60)
                       GTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGG                                                                        <  SRR4302543.6684944/94‑1 (MQ=60)
                       GTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGG                                                                        <  SRR4302543.3946056/94‑1 (MQ=60)
                       GTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGG                                                                        <  SRR4302543.2935748/94‑1 (MQ=60)
                       GTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGG                                                                        >  SRR4302543.2743261/1‑94 (MQ=60)
                        TAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGT                                                                       >  SRR4302543.1396224/1‑94 (MQ=60)
                        TAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGT                                                                       <  SRR4302543.5481640/94‑1 (MQ=60)
                        TAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGT                                                                       <  SRR4302543.5373624/94‑1 (MQ=60)
                        TAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGT                                                                       >  SRR4302543.4356619/1‑94 (MQ=60)
                         AATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTT                                                                      >  SRR4302543.7491888/1‑94 (MQ=60)
                         AATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTT                                                                      <  SRR4302543.1858152/94‑1 (MQ=60)
                          ATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACATAACGCTCTGGTTC                                                                     <  SRR4302543.137953/94‑1 (MQ=60)
                          ATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACATAACGCTCTGGTTC                                                                     <  SRR4302543.137952/94‑1 (MQ=60)
                          ATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTC                                                                     <  SRR4302543.7216746/94‑1 (MQ=60)
                          ATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTC                                                                     <  SRR4302543.5585544/94‑1 (MQ=60)
                            TCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAG                                                                   <  SRR4302543.3298411/94‑1 (MQ=60)
                            ACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAG                                                                   >  SRR4302543.4499952/1‑94 (MQ=60)
                            ACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAG                                                                   <  SRR4302543.3298412/94‑1 (MQ=60)
                              GGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCA                                                                 >  SRR4302543.1351478/1‑94 (MQ=60)
                                CATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAA                                                               >  SRR4302543.3729307/1‑94 (MQ=60)
                                CATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAA                                                               >  SRR4302543.6352678/1‑94 (MQ=60)
                                CATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAA                                                               >  SRR4302543.2930185/1‑94 (MQ=60)
                                CATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAA                                                               >  SRR4302543.1643776/1‑94 (MQ=60)
                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              >  SRR4302543.1022740/1‑94 (MQ=60)
                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              <  SRR4302543.3242955/94‑1 (MQ=60)
                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              <  SRR4302543.7884849/94‑1 (MQ=60)
                                  TTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGT                                                             <  SRR4302543.6961236/94‑1 (MQ=60)
                                  TTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGT                                                             <  SRR4302543.6583182/94‑1 (MQ=60)
                                   TACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGTATCACGTAACAGAACGCTCTGGTTCAGCACAGTA                                                            >  SRR4302543.4124841/1‑94 (MQ=60)
                                    tcggCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAA                                                           <  SRR4302543.810475/90‑1 (MQ=60)
                                    ACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAA                                                           <  SRR4302543.360173/94‑1 (MQ=60)
                                    ACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAA                                                           <  SRR4302543.4680501/94‑1 (MQ=60)
                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302543.1935793/94‑1 (MQ=60)
                                      GCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACG                                                         >  SRR4302543.385350/1‑94 (MQ=60)
                                      GCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACG                                                         <  SRR4302543.1131024/94‑1 (MQ=60)
                                      GCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACG                                                         >  SRR4302543.385351/1‑94 (MQ=60)
                                      GCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACG                                                         <  SRR4302543.362812/94‑1 (MQ=60)
                                      GCCGGCATCGAACAACGCATTACTCAGGTTGGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACG                                                         <  SRR4302543.7207311/94‑1 (MQ=60)
                                       CCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGC                                                        <  SRR4302543.7343481/94‑1 (MQ=60)
                                       CCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGC                                                        <  SRR4302543.4851725/94‑1 (MQ=60)
                                        CGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCC                                                       >  SRR4302543.3043933/1‑94 (MQ=60)
                                        CGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGCTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCC                                                       >  SRR4302543.3592588/1‑94 (MQ=60)
                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      >  SRR4302543.5006627/1‑94 (MQ=60)
                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      <  SRR4302543.1534127/94‑1 (MQ=60)
                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      >  SRR4302543.6334152/1‑94 (MQ=60)
                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      >  SRR4302543.1447739/1‑94 (MQ=60)
                                          GCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTA                                                     <  SRR4302543.60693/94‑1 (MQ=60)
                                          GCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTA                                                     <  SRR4302543.470838/94‑1 (MQ=60)
                                           CATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGTGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    >  SRR4302543.3630434/1‑94 (MQ=60)
                                           CATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    <  SRR4302543.5458871/94‑1 (MQ=60)
                                           CATCGAACAACGCATGACTCAGGTTTGCCCGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    <  SRR4302543.4335832/94‑1 (MQ=60)
                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   >  SRR4302543.1757416/1‑94 (MQ=60)
                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   <  SRR4302543.1422630/94‑1 (MQ=60)
                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   >  SRR4302543.4897857/1‑94 (MQ=60)
                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   >  SRR4302543.4209906/1‑94 (MQ=60)
                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   >  SRR4302543.2896460/1‑94 (MQ=60)
                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   >  SRR4302543.6585921/1‑94 (MQ=60)
                                              CGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCG                                                 >  SRR4302543.674896/1‑94 (MQ=60)
                                              CGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCG                                                 >  SRR4302543.2780894/1‑94 (MQ=60)
                                               GAACAACGCATTACTTAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGC                                                >  SRR4302543.4686197/1‑94 (MQ=60)
                                               GAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTACCGCCTACCCGA                                                >  SRR4302543.3578251/1‑94 (MQ=60)
                                               GAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGG                                                <  SRR4302543.3042522/94‑1 (MQ=60)
                                               GAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGG                                                <  SRR4302543.7127087/94‑1 (MQ=60)
                                               GAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGC                                                >  SRR4302543.784448/1‑94 (MQ=60)
                                                AACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGC                                               >  SRR4302543.451793/1‑94 (MQ=60)
                                                 ACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCG                                              >  SRR4302543.2393494/1‑94 (MQ=60)
                                                  CAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGC                                             >  SRR4302543.539254/1‑94 (MQ=60)
                                                  CAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGC                                             <  SRR4302543.5833877/94‑1 (MQ=60)
                                                   AACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCA                                            <  SRR4302543.7356509/94‑1 (MQ=60)
                                                   AACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCA                                            <  SRR4302543.4037910/94‑1 (MQ=60)
                                                    ACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAA                                           >  SRR4302543.6960801/1‑94 (MQ=60)
                                                    ACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAA                                           >  SRR4302543.4127889/1‑94 (MQ=60)
                                                    ACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAA                                           >  SRR4302543.4127888/1‑94 (MQ=60)
                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          >  SRR4302543.4889802/1‑94 (MQ=60)
                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          <  SRR4302543.2701876/94‑1 (MQ=60)
                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          >  SRR4302543.1760945/1‑94 (MQ=60)
                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          >  SRR4302543.2860082/1‑94 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         <  SRR4302543.2445135/94‑1 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         <  SRR4302543.55935/94‑1 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         <  SRR4302543.2637241/94‑1 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         <  SRR4302543.7639698/94‑1 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         <  SRR4302543.6281805/94‑1 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         <  SRR4302543.5107516/94‑1 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         >  SRR4302543.1918469/1‑94 (MQ=60)
                                                       CATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTT                                        >  SRR4302543.4630022/1‑94 (MQ=60)
                                                        ATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGTTTCAGCAAAGTAACGCCTACCCGGCGCAACTTA                                       >  SRR4302543.7721498/1‑94 (MQ=60)
                                                        ATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTA                                       >  SRR4302543.3479823/1‑94 (MQ=60)
                                                         TTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAG                                      <  SRR4302543.1189141/94‑1 (MQ=60)
                                                         TTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAG                                      <  SRR4302543.6734368/94‑1 (MQ=60)
                                                         TTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAG                                      <  SRR4302543.6245851/94‑1 (MQ=60)
                                                           ACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCC                                    <  SRR4302543.879229/94‑1 (MQ=60)
                                                            CTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCA                                   >  SRR4302543.13170/1‑94 (MQ=60)
                                                             TCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAT                                  <  SRR4302543.4922863/94‑1 (MQ=60)
                                                             TCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAT                                  <  SRR4302543.691503/94‑1 (MQ=60)
                                                             CCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAT                                  <  SRR4302543.10223/94‑1 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 >  SRR4302543.2504263/1‑94 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 <  SRR4302543.3957771/94‑1 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 <  SRR4302543.6310451/94‑1 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 <  SRR4302543.2670021/94‑1 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 <  SRR4302543.2135040/94‑1 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 >  SRR4302543.7746545/1‑94 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAAGATCTGGTTCAGAAAAGTAACGCCTACACGGCGCAACTTAGCCATC                                 >  SRR4302543.2504264/1‑94 (MQ=60)
                                                              CAGGGTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 <  SRR4302543.4699215/94‑1 (MQ=60)
                                                               AGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCG                                <  SRR4302543.5986594/94‑1 (MQ=60)
                                                               AGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCG                                <  SRR4302543.1292890/94‑1 (MQ=60)
                                                               AGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCG                                <  SRR4302543.7245370/94‑1 (MQ=60)
                                                               AGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCG                                <  SRR4302543.368978/94‑1 (MQ=60)
                                                                GGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGC                               >  SRR4302543.2451087/1‑94 (MQ=60)
                                                                GGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGC                               <  SRR4302543.3765764/94‑1 (MQ=60)
                                                                   TTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTG                            <  SRR4302543.7651292/94‑1 (MQ=60)
                                                                   TTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTG                            <  SRR4302543.6673260/94‑1 (MQ=60)
                                                                     GCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAC                          >  SRR4302543.3989999/1‑94 (MQ=60)
                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         >  SRR4302543.6806660/1‑94 (MQ=60)
                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         <  SRR4302543.1703147/94‑1 (MQ=60)
                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         <  SRR4302543.5741775/94‑1 (MQ=60)
                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         >  SRR4302543.6508189/1‑94 (MQ=60)
                                                                         GCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAA                      <  SRR4302543.6320965/94‑1 (MQ=60)
                                                                         GCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAA                      <  SRR4302543.4221263/94‑1 (MQ=60)
                                                                          CGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAT                     <  SRR4302543.794954/94‑1 (MQ=60)
                                                                          CGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAT                     >  SRR4302543.7507302/1‑94 (MQ=60)
                                                                          CGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAT                     <  SRR4302543.4213019/94‑1 (MQ=60)
                                                                           GTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATG                    >  SRR4302543.6788297/1‑94 (MQ=60)
                                                                           GTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATG                    <  SRR4302543.4833222/94‑1 (MQ=60)
                                                                           GTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATG                    >  SRR4302543.6450302/1‑94 (MQ=60)
                                                                           GTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATG                    <  SRR4302543.6013975/94‑1 (MQ=60)
                                                                            TTTGTGCGTTATCTTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGT                   <  SRR4302543.1592516/94‑1 (MQ=60)
                                                                            TTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGT                   <  SRR4302543.5872314/94‑1 (MQ=60)
                                                                             TTGTGCGTTATCGTTCGCATCACGTACCAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  <  SRR4302543.7478474/94‑1 (MQ=60)
                                                                             TTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  >  SRR4302543.1966931/1‑94 (MQ=60)
                                                                             TTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  <  SRR4302543.2615607/94‑1 (MQ=60)
                                                                             TTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  <  SRR4302543.790641/94‑1 (MQ=60)
                                                                              TGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTT                 <  SRR4302543.3911430/94‑1 (MQ=60)
                                                                              TGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTT                 <  SRR4302543.4643178/94‑1 (MQ=60)
                                                                              TGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTT                 <  SRR4302543.7310476/94‑1 (MQ=60)
                                                                              TGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTT                 <  SRR4302543.6619849/94‑1 (MQ=60)
                                                                               GTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTC                <  SRR4302543.445751/94‑1 (MQ=60)
                                                                               GTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTC                <  SRR4302543.6809748/94‑1 (MQ=60)
                                                                                 GCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAT              <  SRR4302543.2978418/94‑1 (MQ=60)
                                                                                 GCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAT              <  SRR4302543.2577593/94‑1 (MQ=60)
                                                                                 GCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAT              <  SRR4302543.6237810/94‑1 (MQ=60)
                                                                                  CGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTGCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATC             >  SRR4302543.1362008/1‑94 (MQ=60)
                                                                                   GTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCT            >  SRR4302543.1134910/1‑94 (MQ=60)
                                                                                    TTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTA           >  SRR4302543.3774850/1‑94 (MQ=60)
                                                                                    TTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTA           <  SRR4302543.7753858/94‑1 (MQ=60)
                                                                                    TTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTA           >  SRR4302543.3358615/1‑94 (MQ=60)
                                                                                    TTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTA           <  SRR4302543.6858006/94‑1 (MQ=60)
                                                                                     TATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAC          <  SRR4302543.6549552/94‑1 (MQ=60)
                                                                                     TATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAC          >  SRR4302543.1918446/1‑94 (MQ=60)
                                                                                     TATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAC          >  SRR4302543.3976001/1‑94 (MQ=60)
                                                                                       TCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCT        >  SRR4302543.3576420/1‑94 (MQ=60)
                                                                                       TCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCT        <  SRR4302543.6525112/94‑1 (MQ=60)
                                                                                       TCGTCCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCT        <  SRR4302543.3127536/94‑1 (MQ=60)
                                                                                        CGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTC       <  SRR4302543.5728998/94‑1 (MQ=60)
                                                                                         GTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA      >  SRR4302543.888809/1‑94 (MQ=60)
                                                                                          TTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGGAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCAT     <  SRR4302543.2854180/94‑1 (MQ=60)
                                                                                           TCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATT    <  SRR4302543.1869559/94‑1 (MQ=60)
                                                                                            CGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTG   >  SRR4302543.7026382/1‑94 (MQ=60)
                                                                                            CGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCCCCTGACGGAATGTTTCATCTACCTCATTG   >  SRR4302543.61046/1‑94 (MQ=60)
                                                                                             GCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  <  SRR4302543.2364703/94‑1 (MQ=60)
                                                                                             GCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  <  SRR4302543.2889168/94‑1 (MQ=60)
                                                                                             GCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  <  SRR4302543.5466182/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  >  NC_000913/4372364‑4372550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑