Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,395,292 T→A S101R (AGT→AGA)  mutL → MutL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,395,2920TA100.0% 688.8 / NA 193S101R (AGT→AGA) mutLMutL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (93/100);  total (93/100)

Only 100 of 206 total aligned reads displayed.

GCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA  >  NC_000913/4395202‑4395381
                                                                                          |                                                                                         
 ctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATc                                                                                        >  2:2261535/1‑93 (MQ=255)
  tggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCa                                                                                       <  1:3412904/93‑1 (MQ=255)
   ggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAg                                                                                      >  2:4654843/1‑93 (MQ=255)
   ggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAg                                                                                      <  1:3208698/93‑1 (MQ=255)
    gcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGt                                                                                     <  1:2503388/93‑1 (MQ=255)
      tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGGATCAGTTc                                                                                   >  2:1623513/1‑93 (MQ=255)
      tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   >  2:3387994/1‑93 (MQ=255)
      tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   <  1:3590149/93‑1 (MQ=255)
      tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   <  1:489320/93‑1 (MQ=255)
      tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   <  1:696817/93‑1 (MQ=255)
          cATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGtt                                                                               <  2:4209292/93‑1 (MQ=255)
             gCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTcc                                                                            >  1:1017598/1‑93 (MQ=255)
             cccaccaGTAAAAACGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCGTTCGCGGTGAGGCGCTGGCGAGATTCAGTTCGGTTTcc                                                                            <  2:2370167/92‑1 (MQ=255)
              ccaccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTccc                                                                           >  1:2966381/1‑93 (MQ=255)
                 ccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTTCCGcc                                                                        >  2:1006723/1‑93 (MQ=255)
                   aGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTg                                                                      >  2:4042157/1‑93 (MQ=255)
                   aGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTg                                                                      >  1:825741/1‑93 (MQ=255)
                    gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGCTCGGTTTTCCGCCTGa                                                                     >  2:3459590/1‑93 (MQ=255)
                     tAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGAc                                                                    <  1:2397353/93‑1 (MQ=255)
                     tAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGAc                                                                    >  1:65233/1‑93 (MQ=255)
                      aaaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACg                                                                   >  1:2993357/1‑93 (MQ=255)
                       aaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGc                                                                  <  1:3755374/93‑1 (MQ=255)
                          tCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTcc                                                               >  1:2623736/1‑92 (MQ=255)
                           cGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCAc                                                              <  2:3010075/93‑1 (MQ=255)
                            gCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACt                                                             >  2:3050462/1‑93 (MQ=255)
                            gCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACt                                                             >  1:2883883/1‑93 (MQ=255)
                              ctctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTc                                                           >  2:2337931/1‑93 (MQ=255)
                              ctctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTc                                                           >  2:4176972/1‑93 (MQ=255)
                              ctatctGGAAGATCTCGACGCAATTATCACCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGCTTCCCGCCTGACGCTCACTTc                                                           <  2:3963642/90‑1 (MQ=255)
                               tctctGGCCGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa                                                          <  1:586875/93‑1 (MQ=255)
                               tctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa                                                          >  1:3626527/1‑93 (MQ=255)
                               tctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGGTTCGCGCCTGACGCTCACTTCa                                                          >  2:4347417/1‑93 (MQ=255)
                                 tctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACg                                                        >  2:4474614/1‑93 (MQ=255)
                                   tGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCa                                                      <  2:2924850/93‑1 (MQ=255)
                                     gACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAcc                                                    >  1:3276853/1‑93 (MQ=255)
                                     gACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAcc                                                    >  1:1428948/1‑93 (MQ=255)
                                     gACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAcc                                                    <  2:3264939/93‑1 (MQ=255)
                                      aCGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGGACCg                                                   >  2:1770329/1‑93 (MQ=255)
                                      aCGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCg                                                   >  1:190471/1‑93 (MQ=255)
                                       cGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGc                                                  >  1:2225813/1‑93 (MQ=255)
                                       cGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGc                                                  >  2:743487/1‑93 (MQ=255)
                                          tctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGa                                               >  2:504186/1‑93 (MQ=255)
                                           ctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGaa                                              <  2:1670885/93‑1 (MQ=255)
                                            tcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAc                                             >  2:3355318/1‑93 (MQ=255)
                                            tcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAc                                             <  2:1988566/93‑1 (MQ=255)
                                             cGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAca                                            <  2:2166897/93‑1 (MQ=255)
                                               aaGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGATAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagc                                          >  1:2515038/1‑93 (MQ=255)
                                               aaGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagc                                          >  2:251875/1‑93 (MQ=255)
                                                aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagca                                         >  2:5498/1‑93 (MQ=255)
                                                   cATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGa                                      >  1:1997812/1‑93 (MQ=255)
                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     <  1:4254927/93‑1 (MQ=255)
                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     <  2:2333111/93‑1 (MQ=255)
                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     >  2:4405501/1‑93 (MQ=255)
                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     >  2:3289665/1‑93 (MQ=255)
                                                     ttATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAg                                    >  2:3784155/1‑93 (MQ=255)
                                                     ttATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAg                                    <  1:4520548/93‑1 (MQ=255)
                                                      tATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGc                                   <  1:3088261/93‑1 (MQ=255)
                                                       ttCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGcc                                  <  2:3633426/92‑1 (MQ=255)
                                                       aTCAGCCTGGGCTTTCGCGGTTAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGcc                                  <  2:4232705/93‑1 (MQ=255)
                                                       aTCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGcc                                  <  1:869863/93‑1 (MQ=255)
                                                       aTCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGcc                                  <  1:458881/93‑1 (MQ=255)
                                                       aTCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGcc                                  <  1:1698773/93‑1 (MQ=255)
                                                        tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 >  2:2114876/1‑93 (MQ=255)
                                                        tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 >  1:3113105/1‑93 (MQ=255)
                                                          aGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTgg                               <  2:281901/93‑1 (MQ=255)
                                                           gCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGc                              <  1:612978/93‑1 (MQ=255)
                                                            ccTGGGCTTTCGCGGTGAGGCGCTTGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  1:2788361/93‑1 (MQ=255)
                                                            ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  2:4531862/93‑1 (MQ=255)
                                                            ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  1:4132704/93‑1 (MQ=255)
                                                             cTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGACGCCTGGCAg                            >  1:3937705/1‑93 (MQ=255)
                                                             cTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAg                            >  1:1565234/1‑93 (MQ=255)
                                                              tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg                           >  1:1288867/1‑93 (MQ=255)
                                                              tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg                           >  1:71060/1‑93 (MQ=255)
                                                               gggCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGc                          >  2:2459695/1‑93 (MQ=255)
                                                               gggCTTGCGCGCTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGc                          <  2:204128/93‑1 (MQ=255)
                                                                 gCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCt                        >  2:2693286/1‑93 (MQ=255)
                                                                  cTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTa                       <  2:49612/93‑1 (MQ=255)
                                                                  cTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTa                       <  2:1344782/93‑1 (MQ=255)
                                                                   tttCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTAt                      >  2:3583298/1‑93 (MQ=255)
                                                                    ttCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATg                     <  2:48083/93‑1 (MQ=255)
                                                                     tCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGc                    <  2:3816870/93‑1 (MQ=255)
                                                                      cgcgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGcc                   <  1:503878/93‑1 (MQ=255)
                                                                       gcgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCg                  >  1:4056666/1‑93 (MQ=255)
                                                                         ggggAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                <  1:4318737/90‑1 (MQ=255)
                                                                          gTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAg               <  1:3859357/93‑1 (MQ=255)
                                                                          gTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAg               <  1:2426091/93‑1 (MQ=255)
                                                                          gTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAg               >  1:656905/1‑93 (MQ=255)
                                                                          gTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAg               >  1:2134759/1‑93 (MQ=255)
                                                                           tGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAgg              >  2:4506197/1‑93 (MQ=255)
                                                                           tGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAgg              <  1:1330943/93‑1 (MQ=255)
                                                                            gAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAggg             <  1:3994187/93‑1 (MQ=255)
                                                                            gAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAggg             <  1:3603458/93‑1 (MQ=255)
                                                                             gggCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgc            <  1:939702/92‑1 (MQ=255)
                                                                              ggCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgcg           >  2:1116507/1‑93 (MQ=255)
                                                                              ggCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgcg           >  2:2593299/1‑93 (MQ=255)
                                                                                   tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGata      <  2:3027262/93‑1 (MQ=255)
                                                                                    ggCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGatat     <  1:2357737/93‑1 (MQ=255)
                                                                                     gCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATg    >  2:3152074/1‑93 (MQ=255)
                                                                                      cGAGAATCAGTTCGGTTACCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGa   <  2:3519667/93‑1 (MQ=255)
                                                                                       gagaATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGaa  <  2:2995478/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA  >  NC_000913/4395202‑4395381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGT  >  NC_000913/4395200‑4395384
                                                                                            |                                                                                            
GCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAA                                                                                             <  SRR4302535.4644238/94‑1 (MQ=60)
 CGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAAT                                                                                            <  SRR4302535.2513132/94‑1 (MQ=60)
 CGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAAT                                                                                            <  SRR4302535.4399279/94‑1 (MQ=60)
 CGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAAT                                                                                            <  SRR4302535.1712815/94‑1 (MQ=60)
  GCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATC                                                                                           <  SRR4302535.4035610/94‑1 (MQ=60)
  GCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATC                                                                                           <  SRR4302535.549445/94‑1 (MQ=60)
    TGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAG                                                                                         <  SRR4302535.3436652/94‑1 (MQ=60)
     GGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGT                                                                                        <  SRR4302535.4017548/94‑1 (MQ=60)
     GGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGT                                                                                        <  SRR4302535.3230922/94‑1 (MQ=60)
      GCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTT                                                                                       <  SRR4302535.2521438/94‑1 (MQ=60)
       CTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTC                                                                                      >  SRR4302535.3728458/1‑94 (MQ=60)
       CTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATAAGTTC                                                                                      >  SRR4302535.609929/1‑94 (MQ=60)
        TCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCG                                                                                     <  SRR4302535.492756/94‑1 (MQ=60)
        TCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCG                                                                                     <  SRR4302535.3615051/94‑1 (MQ=60)
        TCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCG                                                                                     <  SRR4302535.701703/94‑1 (MQ=60)
          GTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGT                                                                                   >  SRR4302535.4569890/1‑94 (MQ=60)
          GTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGT                                                                                   <  SRR4302535.3533200/94‑1 (MQ=60)
          GTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGT                                                                                   >  SRR4302535.2144840/1‑94 (MQ=60)
            CATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGATAATCAGTTCGGTTT                                                                                 <  SRR4302535.2402851/94‑1 (MQ=60)
            CATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTT                                                                                 <  SRR4302535.4337378/94‑1 (MQ=60)
            CATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTT                                                                                 <  SRR4302535.1788523/94‑1 (MQ=60)
            CATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTT                                                                                 <  SRR4302535.1456704/94‑1 (MQ=60)
             ATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTC                                                                                >  SRR4302535.665720/1‑94 (MQ=60)
             ATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTC                                                                                <  SRR4302535.2850690/94‑1 (MQ=60)
              TGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCC                                                                               >  SRR4302535.1024831/1‑94 (MQ=60)
               GCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCC                                                                              >  SRR4302535.2987192/1‑94 (MQ=60)
                CCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTGGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCG                                                                             >  SRR4302535.3003347/1‑94 (MQ=60)
                 CACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGC                                                                            >  SRR4302535.4495448/1‑94 (MQ=60)
                    CAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTG                                                                         <  SRR4302535.978416/94‑1 (MQ=60)
                    CAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTG                                                                         >  SRR4302535.831524/1‑94 (MQ=60)
                      GTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGAC                                                                       >  SRR4302535.65724/1‑94 (MQ=60)
                      GTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGAC                                                                       >  SRR4302535.3631880/1‑94 (MQ=60)
                       TAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACG                                                                      >  SRR4302535.3014346/1‑94 (MQ=60)
                       TAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACG                                                                      <  SRR4302535.2414583/94‑1 (MQ=60)
                         AAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCT                                                                    <  SRR4302535.3781349/94‑1 (MQ=60)
                           ATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCC                                                                  >  SRR4302535.2642538/1‑94 (MQ=60)
                             CGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACT                                                                >  SRR4302535.3225260/1‑94 (MQ=60)
                             CGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACT                                                                >  SRR4302535.2904290/1‑94 (MQ=60)
                                CTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCC                                                             >  SRR4302535.3059780/1‑94 (MQ=60)
                                CTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCA                                                             >  SRR4302535.3651658/1‑94 (MQ=60)
                                 TCTCTGGCCGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCAC                                                            <  SRR4302535.590986/94‑1 (MQ=60)
                                   CCTGGACGATCTCGAAGCCATTATCAGCCTGGCCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGC                                                          <  SRR4302535.1603943/94‑1 (MQ=60)
                                      GGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACC                                                       >  SRR4302535.3299616/1‑94 (MQ=60)
                                      GGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACC                                                       >  SRR4302535.1439145/1‑94 (MQ=60)
                                       GACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCG                                                      >  SRR4302535.191785/1‑94 (MQ=60)
                                        ACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGC                                                     >  SRR4302535.2241648/1‑94 (MQ=60)
                                          GATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAG                                                   >  SRR4302535.3234362/1‑94 (MQ=60)
                                           ATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGA                                                  >  SRR4302535.3353037/1‑94 (MQ=60)
                                             CTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAC                                                >  SRR4302535.500357/1‑94 (MQ=60)
                                                GAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGATAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGC                                             >  SRR4302535.2533170/1‑94 (MQ=60)
                                                 AAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCA                                            >  SRR4302535.4199625/1‑94 (MQ=60)
                                                  AGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAG                                           <  SRR4302535.1955194/94‑1 (MQ=60)
                                                  AGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTAGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAG                                           <  SRR4302535.2529523/94‑1 (MQ=60)
                                                   GCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGG                                          <  SRR4302535.1313997/94‑1 (MQ=60)
                                                    CCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGA                                         >  SRR4302535.2012199/1‑94 (MQ=60)
                                                     CATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAA                                        >  SRR4302535.2666179/1‑94 (MQ=60)
                                                      ATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAG                                       <  SRR4302535.4228044/94‑1 (MQ=60)
                                                      ATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAG                                       <  SRR4302535.4284076/94‑1 (MQ=60)
                                                       TTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGC                                      <  SRR4302535.4551530/94‑1 (MQ=60)
                                                        TATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCC                                     <  SRR4302535.3109770/94‑1 (MQ=60)
                                                         ATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCT                                    >  SRR4302535.3134777/1‑94 (MQ=60)
                                                         ATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCT                                    <  SRR4302535.462091/94‑1 (MQ=60)
                                                         ATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCT                                    <  SRR4302535.1711050/94‑1 (MQ=60)
                                                         ATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCT                                    <  SRR4302535.876026/94‑1 (MQ=60)
                                                           CAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGG                                  <  SRR4302535.1410810/94‑1 (MQ=60)
                                                           CAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGG                                  <  SRR4302535.3616767/94‑1 (MQ=60)
                                                           CAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGG                                  <  SRR4302535.789513/94‑1 (MQ=60)
                                                            CGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGC                                 <  SRR4302535.843137/94‑1 (MQ=60)
                                                             GCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCA                                <  SRR4302535.617241/94‑1 (MQ=60)
                                                              CCTGGGCTTTCGCGGTGAGGCGCTTGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               <  SRR4302535.2808204/94‑1 (MQ=60)
                                                              CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGACGCCTGGCAG                               >  SRR4302535.3964560/1‑94 (MQ=60)
                                                              CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               <  SRR4302535.704471/94‑1 (MQ=60)
                                                              CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               >  SRR4302535.1576536/1‑94 (MQ=60)
                                                              CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               >  SRR4302535.108440/1‑94 (MQ=60)
                                                              CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               <  SRR4302535.4160979/94‑1 (MQ=60)
                                                              CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               >  SRR4302535.2857068/1‑94 (MQ=60)
                                                              CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               >  SRR4302535.2943052/1‑94 (MQ=60)
                                                               CTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGG                              >  SRR4302535.1298049/1‑94 (MQ=60)
                                                               CTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGG                              >  SRR4302535.71582/1‑94 (MQ=60)
                                                                 GGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCC                            >  SRR4302535.653771/1‑94 (MQ=60)
                                                                   GCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTA                          >  SRR4302535.2301818/1‑94 (MQ=60)
                                                                       TCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCC                      >  SRR4302535.2603005/1‑94 (MQ=60)
                                                                        CGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCG                     >  SRR4302535.4084438/1‑94 (MQ=60)
                                                                        CGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCG                     <  SRR4302535.507399/94‑1 (MQ=60)
                                                                         GCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGA                    >  SRR4302535.312226/1‑94 (MQ=60)
                                                                          CGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAA                   >  SRR4302535.4636438/1‑94 (MQ=60)
                                                                           GGTGAGGCGCTGGCGAGAATCGGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAG                  >  SRR4302535.2458995/1‑94 (MQ=60)
                                                                           GGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAG                  >  SRR4302535.2150141/1‑94 (MQ=60)
                                                                           GGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAG                  <  SRR4302535.2440848/94‑1 (MQ=60)
                                                                           GGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAG                  >  SRR4302535.661490/1‑94 (MQ=60)
                                                                           GGGGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAG                  <  SRR4302535.4348281/94‑1 (MQ=60)
                                                                            GTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGG                 >  SRR4302535.4139505/1‑94 (MQ=60)
                                                                            GTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGG                 <  SRR4302535.2443570/94‑1 (MQ=60)
                                                                            GTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGG                 <  SRR4302535.3885919/94‑1 (MQ=60)
                                                                             TGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGG                <  SRR4302535.1340438/94‑1 (MQ=60)
                                                                              GAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGC               <  SRR4302535.3628461/94‑1 (MQ=60)
                                                                              GAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGC               <  SRR4302535.4021510/94‑1 (MQ=60)
                                                                               GGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCG              <  SRR4302535.946358/94‑1 (MQ=60)
                                                                                 GCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCG            <  SRR4302535.658660/94‑1 (MQ=60)
                                                                                     TGGCGAAAATCAGTGCGATTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATAT        <  SRR4302535.3148096/94‑1 (MQ=60)
                                                                                      GGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATG       >  SRR4302535.396010/1‑94 (MQ=60)
                                                                                      GGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATG       <  SRR4302535.2374648/94‑1 (MQ=60)
                                                                                        CGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA     <  SRR4302535.925107/94‑1 (MQ=60)
                                                                                        CGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGAGCGATATGAA     >  SRR4302535.2197773/1‑94 (MQ=60)
                                                                                        CGAGAATCACTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA     <  SRR4302535.3877144/94‑1 (MQ=60)
                                                                                         GAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAAC    >  SRR4302535.3253222/1‑94 (MQ=60)
                                                                                          AGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACG   <  SRR4302535.2195730/94‑1 (MQ=60)
                                                                                          AGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACG   <  SRR4302535.2195731/94‑1 (MQ=60)
                                                                                           GAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGT  <  SRR4302535.4290902/94‑1 (MQ=60)
                                                                                           GAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGT  >  SRR4302535.3770908/1‑94 (MQ=60)
                                                                                           GAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGT  <  SRR4302535.3674690/94‑1 (MQ=60)
                                                                                            |                                                                                            
GCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGT  >  NC_000913/4395200‑4395384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑