Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,414,467 A→G intergenic (‑119/‑63) b4189 ← / → yjfP hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,414,4670AG99.5% 697.0 / NA 219intergenic (‑119/‑63)b4189/yjfPhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (102/116);  minor base T (0/1);  total (102/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

GGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATACCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTG  >  NC_000913/4414377‑4414556
                                                                                          |                                                                                         
ggggAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGcc                                                                                         >  2:1354813/1‑93 (MQ=255)
ggggAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGcc                                                                                         <  2:1222812/93‑1 (MQ=255)
ggggAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGcc                                                                                         >  2:1333117/1‑93 (MQ=255)
 gggAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCg                                                                                        >  2:3967709/1‑93 (MQ=255)
  ggAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGc                                                                                       <  1:361975/93‑1 (MQ=255)
   gagtGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATgccgcc                                                                                      >  1:3870180/4‑93 (MQ=255)
   gAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATgccgcc                                                                                      <  2:3670714/93‑1 (MQ=255)
   gAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATgccgcc                                                                                      >  1:1987034/1‑93 (MQ=255)
    aaTGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCca                                                                                     <  1:4154617/93‑1 (MQ=255)
     aTGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCcag                                                                                    <  1:4408325/93‑1 (MQ=255)
       gTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCcagca                                                                                  <  1:1661440/93‑1 (MQ=255)
       gTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCcagca                                                                                  <  2:4194067/93‑1 (MQ=255)
       gTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCcagca                                                                                  >  2:2806558/1‑93 (MQ=255)
       gTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCcagca                                                                                  >  2:3118501/1‑93 (MQ=255)
           ccGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGaaa                                                                              <  1:809996/93‑1 (MQ=255)
            cGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAAc                                                                             >  1:1721247/1‑93 (MQ=255)
            cGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAAc                                                                             <  2:4659088/93‑1 (MQ=255)
             gAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACt                                                                            >  2:2719496/1‑93 (MQ=255)
             gAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACt                                                                            >  1:4405700/1‑93 (MQ=255)
              aaTTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACtt                                                                           >  1:1534139/1‑93 (MQ=255)
                ttGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTtgt                                                                         >  1:1552070/1‑93 (MQ=255)
                  tttACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGc                                                                       <  1:3980509/92‑1 (MQ=255)
                  gTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGc                                                                       >  1:2654768/1‑93 (MQ=255)
                  gTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGc                                                                       >  1:3142/1‑93 (MQ=255)
                      ccTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAg                                                                   <  2:3221249/93‑1 (MQ=255)
                       cTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAgg                                                                  >  1:988257/1‑93 (MQ=255)
                       cTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAgg                                                                  <  1:1939932/93‑1 (MQ=255)
                       cTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAgg                                                                  >  1:4030228/1‑93 (MQ=255)
                        tGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGa                                                                 <  2:3920244/93‑1 (MQ=255)
                        tGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGa                                                                 <  2:2619560/93‑1 (MQ=255)
                         ggTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGAt                                                                >  2:4084207/1‑93 (MQ=255)
                         ggTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGAt                                                                >  2:1425686/1‑93 (MQ=255)
                          gTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGAtt                                                               <  2:1854306/93‑1 (MQ=255)
                           tACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGAttt                                                              >  2:2577093/1‑93 (MQ=255)
                             cgcgATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATttct                                                            <  1:2576243/93‑1 (MQ=255)
                              gcgATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATttctt                                                           <  2:4629855/93‑1 (MQ=255)
                                 aTTTTCAAATGAAAACGTGACGAAAGCGTAGCCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCAt                                                        <  2:1107296/93‑1 (MQ=255)
                                  ttttCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATg                                                       <  2:2341504/93‑1 (MQ=255)
                                  ttttCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATg                                                       <  2:1515916/93‑1 (MQ=255)
                                  ttttCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATg                                                       >  1:1746630/1‑93 (MQ=255)
                                   tttCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGt                                                      >  1:354998/1‑93 (MQ=255)
                                   tttCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGt                                                      >  1:1037208/1‑93 (MQ=255)
                                   tttCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGt                                                      <  1:2583227/93‑1 (MQ=255)
                                     tCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCa                                                    <  2:3330302/93‑1 (MQ=255)
                                     tCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCa                                                    <  2:777824/93‑1 (MQ=255)
                                      cGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAAGTTGTGCTAAGGATTTCTTCATGTCAt                                                   >  2:196028/1‑93 (MQ=255)
                                       gAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATa                                                  >  2:2662514/1‑93 (MQ=255)
                                        aaTGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATAc                                                 <  1:2658598/93‑1 (MQ=255)
                                         aTGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATAcc                                                >  1:1279816/1‑93 (MQ=255)
                                             aaaCGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGAc                                            <  2:4208580/93‑1 (MQ=255)
                                              aaCGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACa                                           >  1:2953096/1‑93 (MQ=255)
                                               aCGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACaa                                          <  2:1044055/93‑1 (MQ=255)
                                                  tGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACaaaaa                                       <  2:1543629/93‑1 (MQ=255)
                                                    aCGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAgg                                     >  2:555762/1‑93 (MQ=255)
                                                     cGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGga                                    <  1:2290053/93‑1 (MQ=255)
                                                     cGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGga                                    <  1:3257001/93‑1 (MQ=255)
                                                     cGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGga                                    <  2:2440227/93‑1 (MQ=255)
                                                       aaaGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCCGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGgaga                                  >  1:1111061/1‑93 (MQ=255)
                                                       aaaGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGgaga                                  <  1:4598229/93‑1 (MQ=255)
                                                       aaaGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGgaga                                  <  1:3770920/93‑1 (MQ=255)
                                                          gCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGAc                               >  2:211811/1‑93 (MQ=255)
                                                          gCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGAc                               <  2:2658195/93‑1 (MQ=255)
                                                          gCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGAc                               <  2:448389/93‑1 (MQ=255)
                                                          acGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGTTTTCTTCATGTCATTCCAGACAAAACGGAGAGAc                               <  2:4580888/92‑1 (MQ=255)
                                                           cGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACa                              <  2:3139510/93‑1 (MQ=255)
                                                              aaCCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAgat                           >  1:1050213/1‑93 (MQ=255)
                                                                cccACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAgatga                         >  2:289815/1‑93 (MQ=255)
                                                                cccACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAgatga                         <  2:3876826/93‑1 (MQ=255)
                                                                 ccACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAgatgat                        >  1:3343803/1‑93 (MQ=255)
                                                                 ccACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAgatgat                        <  1:1792534/93‑1 (MQ=255)
                                                                 ccACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAgatgat                        >  2:3494040/1‑93 (MQ=255)
                                                                  cacaTCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGAtt                       >  2:1606426/1‑93 (MQ=255)
                                                                  cacaTCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGAtt                       >  2:4281186/1‑93 (MQ=255)
                                                                  cacaTCTCAACATTATGAACTAATGCAGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGAtt                       >  2:3453263/1‑93 (MQ=255)
                                                                   acaTCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTg                      <  1:3215257/93‑1 (MQ=255)
                                                                    caTCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGa                     <  1:4040977/93‑1 (MQ=255)
                                                                    caTCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGa                     >  1:2753321/1‑93 (MQ=255)
                                                                    aaTCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGa                     <  1:4209442/92‑1 (MQ=255)
                                                                     aTCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGaa                    <  2:595824/93‑1 (MQ=255)
                                                                       ctcAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAAt                  <  1:1952570/93‑1 (MQ=255)
                                                                        tcAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATa                 <  2:693514/93‑1 (MQ=255)
                                                                        tcAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATa                 >  1:4488562/1‑93 (MQ=255)
                                                                        tcAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATa                 >  2:888179/1‑93 (MQ=255)
                                                                         cAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAg                >  1:704950/1‑93 (MQ=255)
                                                                          aaCATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGa               <  2:4601197/93‑1 (MQ=255)
                                                                          aaCATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGa               <  2:4130994/93‑1 (MQ=255)
                                                                          aaCATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGa               >  2:362143/1‑93 (MQ=255)
                                                                           aCATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGaa              >  1:4114257/1‑93 (MQ=255)
                                                                           aCATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGaa              <  1:620539/93‑1 (MQ=255)
                                                                           aCATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGaa              <  1:206762/93‑1 (MQ=255)
                                                                             aTTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATc            <  2:1325925/93‑1 (MQ=255)
                                                                               tATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCAc          <  1:1445822/93‑1 (MQ=255)
                                                                                 tGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCAcgc        <  2:2310143/93‑1 (MQ=255)
                                                                                  gAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCAcgcg       >  2:4388682/1‑93 (MQ=255)
                                                                                  gAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCAcgcg       >  1:3447462/1‑93 (MQ=255)
                                                                                  gAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCAcgcg       <  1:4305936/93‑1 (MQ=255)
                                                                                    aCTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAg     >  1:2407378/1‑93 (MQ=255)
                                                                                    aCTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAg     >  1:2464268/1‑93 (MQ=255)
                                                                                     cTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGc    >  2:2511026/1‑93 (MQ=255)
                                                                                       aaTGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTg  <  2:2257708/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATACCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTG  >  NC_000913/4414377‑4414556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATACCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCA  >  NC_000913/4414374‑4414559
                                                                                             |                                                                                            
TTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATG                                                                                              <  SRR4302535.2055015/94‑1 (MQ=60)
TTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATG                                                                                              <  SRR4302535.4381556/94‑1 (MQ=60)
TTGGGGGAATGTTCCCGAATTGTTACCTGGTACGAGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATG                                                                                              >  SRR4302535.1351534/1‑94 (MQ=60)
 TGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGC                                                                                             >  SRR4302535.1875554/1‑94 (MQ=60)
  ctgaaccgctcttccgatctgcgtggtGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCC                                                                                            <  SRR4302535.2941000/67‑1 (MQ=60)
  GGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCC                                                                                            <  SRR4302535.4620460/94‑1 (MQ=60)
     GGAGTGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCC                                                                                         >  SRR4302535.3896779/1‑94 (MQ=60)
     GGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCC                                                                                         >  SRR4302535.2001343/1‑94 (MQ=60)
     GGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCC                                                                                         <  SRR4302535.364472/94‑1 (MQ=60)
       AATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAG                                                                                       <  SRR4302535.4183062/94‑1 (MQ=60)
        ATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGC                                                                                      <  SRR4302535.4438490/94‑1 (MQ=60)
        ATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGC                                                                                      >  SRR4302535.4222809/1‑94 (MQ=60)
        ATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGC                                                                                      <  SRR4302535.3138435/94‑1 (MQ=60)
        ATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGC                                                                                      >  SRR4302535.2447983/1‑94 (MQ=60)
          GTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAG                                                                                    >  SRR4302535.4516576/1‑94 (MQ=60)
          GTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAG                                                                                    >  SRR4302535.4619235/1‑94 (MQ=60)
          GTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAG                                                                                    >  SRR4302535.1971532/1‑94 (MQ=60)
          GTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAG                                                                                    >  SRR4302535.3265061/1‑94 (MQ=60)
          GTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAG                                                                                    <  SRR4302535.1673445/94‑1 (MQ=60)
           TTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTCACCAACATCTCAACATTATGAACTAATGCCGCCAGCAGA                                                                                   >  SRR4302535.4094802/1‑94 (MQ=60)
           TTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGA                                                                                   <  SRR4302535.1735325/94‑1 (MQ=60)
            TCCCGAATTGTTGCCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTACCCCACATCTCACCATTATGAACTAATGCCGCCAGCAGAA                                                                                  <  SRR4302535.1689303/94‑1 (MQ=60)
             CCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAA                                                                                 >  SRR4302535.3406992/1‑94 (MQ=60)
              CCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAAC                                                                                >  SRR4302535.1733693/1‑94 (MQ=60)
              CCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAAC                                                                                <  SRR4302535.3573174/94‑1 (MQ=60)
              CCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAAC                                                                                <  SRR4302535.815660/94‑1 (MQ=60)
               CGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACT                                                                               <  SRR4302535.4693718/94‑1 (MQ=60)
               CGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACT                                                                               >  SRR4302535.4435852/1‑94 (MQ=60)
                GAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTT                                                                              >  SRR4302535.2008192/1‑94 (MQ=60)
                GAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTT                                                                              >  SRR4302535.1545192/1‑94 (MQ=60)
                 AATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTG                                                                             <  SRR4302535.713069/94‑1 (MQ=60)
                  ATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGT                                                                            >  SRR4302535.1563251/1‑94 (MQ=60)
                  ATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGT                                                                            <  SRR4302535.652644/94‑1 (MQ=60)
                  ATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGT                                                                            <  SRR4302535.3166788/94‑1 (MQ=60)
                   ATGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTG                                                                           <  SRR4302535.3778223/94‑1 (MQ=60)
                    TGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGC                                                                          >  SRR4302535.2673790/1‑94 (MQ=60)
                    TGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGC                                                                          >  SRR4302535.3171/1‑94 (MQ=60)
                     TTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCT                                                                         <  SRR4302535.4007703/94‑1 (MQ=60)
                         CCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGG                                                                     >  SRR4302535.995278/1‑94 (MQ=60)
                         CCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGG                                                                     >  SRR4302535.4057802/1‑94 (MQ=60)
                          CTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGA                                                                    <  SRR4302535.1953905/94‑1 (MQ=60)
                                CGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTT                                                              <  SRR4302535.2594759/94‑1 (MQ=60)
                                CGCGATTTTCGAATGAAAACGTAACAAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTT                                                              <  SRR4302535.3461194/94‑1 (MQ=60)
                                   GATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCAT                                                           <  SRR4302535.105499/94‑1 (MQ=60)
                                    ATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATG                                                          <  SRR4302535.1113078/94‑1 (MQ=60)
                                    ATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATG                                                          >  SRR4302535.1759265/1‑94 (MQ=60)
                                     TTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGT                                                         >  SRR4302535.1044574/1‑94 (MQ=60)
                                     TTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGT                                                         >  SRR4302535.357449/1‑94 (MQ=60)
                                      TTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTC                                                        <  SRR4302535.2601787/94‑1 (MQ=60)
                                        TCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCAT                                                      >  SRR4302535.2573160/1‑94 (MQ=60)
                                        TCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCAT                                                      <  SRR4302535.3201119/94‑1 (MQ=60)
                                         CGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATA                                                     >  SRR4302535.119348/1‑94 (MQ=60)
                                          GAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATAC                                                    >  SRR4302535.2985135/1‑94 (MQ=60)
                                           AATGAAAACTTGACGAAAGCGTAACCCACATCTCACCATTATGAACTAAAGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACC                                                   <  SRR4302535.2677646/94‑1 (MQ=60)
                                           AATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACC                                                   >  SRR4302535.1288953/1‑94 (MQ=60)
                                           AATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACC                                                   <  SRR4302535.2677647/94‑1 (MQ=60)
                                                AAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACA                                              >  SRR4302535.2973844/1‑94 (MQ=60)
                                                 AACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAA                                             >  SRR4302535.2883649/1‑94 (MQ=60)
                                                   CGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAA                                           >  SRR4302535.2551478/1‑94 (MQ=60)
                                                   CGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAA                                           >  SRR4302535.2599230/1‑94 (MQ=60)
                                                    GTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAA                                          >  SRR4302535.4022742/1‑94 (MQ=60)
                                                     TGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAG                                         >  SRR4302535.2104861/1‑94 (MQ=60)
                                                        CGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAG                                      <  SRR4302535.2306430/94‑1 (MQ=60)
                                                        CGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAG                                      <  SRR4302535.4396456/94‑1 (MQ=60)
                                                        CGAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAG                                      <  SRR4302535.3279631/94‑1 (MQ=60)
                                                         GAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCCGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGA                                     >  SRR4302535.1118990/1‑94 (MQ=60)
                                                         GAAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGA                                     >  SRR4302535.4345208/1‑94 (MQ=60)
                                                          AAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAG                                    <  SRR4302535.4629510/94‑1 (MQ=60)
                                                          AAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAG                                    <  SRR4302535.3885642/94‑1 (MQ=60)
                                                          AAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAG                                    <  SRR4302535.3796939/94‑1 (MQ=60)
                                                          AAAGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAG                                    <  SRR4302535.4251339/94‑1 (MQ=60)
                                                            AGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGAC                                  >  SRR4302535.1940105/1‑94 (MQ=60)
                                                            AGCGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGAC                                  >  SRR4302535.217754/1‑94 (MQ=60)
                                                              CGTAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAG                                >  SRR4302535.1694727/1‑94 (MQ=60)
                                                                TATCCCACATCTCAACATTATGACCTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGAT                              <  SRR4302535.1551524/94‑1 (MQ=60)
                                                                TAACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGAT                              >  SRR4302535.1057681/1‑94 (MQ=60)
                                                                 AACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATG                             >  SRR4302535.2683502/1‑94 (MQ=60)
                                                                 AACCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATG                             <  SRR4302535.1615416/94‑1 (MQ=60)
                                                                   CCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGAT                           >  SRR4302535.859309/1‑94 (MQ=60)
                                                                   CCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGAT                           >  SRR4302535.3367099/1‑94 (MQ=60)
                                                                   CCCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGAT                           <  SRR4302535.3304589/94‑1 (MQ=60)
                                                                    CCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATT                          <  SRR4302535.1805493/94‑1 (MQ=60)
                                                                    CCACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATT                          <  SRR4302535.129936/94‑1 (MQ=60)
                                                                     CACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTG                         <  SRR4302535.4437198/94‑1 (MQ=60)
                                                                     CACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTG                         >  SRR4302535.848451/1‑94 (MQ=60)
                                                                      ACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGA                        >  SRR4302535.2772943/1‑94 (MQ=60)
                                                                      ACATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGA                        <  SRR4302535.3237525/94‑1 (MQ=60)
                                                                       CATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAA                       <  SRR4302535.4068641/94‑1 (MQ=60)
                                                                       AATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAA                       <  SRR4302535.4238251/94‑1 (MQ=60)
                                                                        ATCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAA                      >  SRR4302535.1961543/1‑94 (MQ=60)
                                                                         TCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAAT                     <  SRR4302535.2640249/94‑1 (MQ=60)
                                                                         TCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAAT                     <  SRR4302535.2721457/94‑1 (MQ=60)
                                                                         TCTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAAT                     <  SRR4302535.4156808/94‑1 (MQ=60)
                                                                          CTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATA                    >  SRR4302535.4519375/1‑94 (MQ=60)
                                                                          CTCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATA                    <  SRR4302535.1966624/94‑1 (MQ=60)
                                                                           TCAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAG                   >  SRR4302535.709899/1‑94 (MQ=60)
                                                                            CAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGA                  <  SRR4302535.1799741/94‑1 (MQ=60)
                                                                            CAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGA                  >  SRR4302535.2361327/1‑94 (MQ=60)
                                                                            CAACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGA                  >  SRR4302535.3577707/1‑94 (MQ=60)
                                                                             AACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAA                 >  SRR4302535.4142417/1‑94 (MQ=60)
                                                                              ACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAAT                <  SRR4302535.208180/94‑1 (MQ=60)
                                                                              ACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAAT                <  SRR4302535.624852/94‑1 (MQ=60)
                                                                              ACATTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAAT                <  SRR4302535.3485284/94‑1 (MQ=60)
                                                                              ACATTATGAACTAATGCCGCCAGCAGAAACGTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAAT                <  SRR4302535.454550/94‑1 (MQ=60)
                                                                              ACAGTATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAAT                <  SRR4302535.1309664/94‑1 (MQ=60)
                                                                                  TATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACG            >  SRR4302535.3990759/1‑94 (MQ=60)
                                                                                  TATGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACG            <  SRR4302535.1456161/94‑1 (MQ=60)
                                                                                    TGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCG          >  SRR4302535.3471433/1‑94 (MQ=60)
                                                                                    TGAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCG          <  SRR4302535.4108572/94‑1 (MQ=60)
                                                                                     GAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGA         >  SRR4302535.1929211/1‑94 (MQ=60)
                                                                                     GAACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGA         <  SRR4302535.4335396/94‑1 (MQ=60)
                                                                                      AACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAG        >  SRR4302535.2424706/1‑94 (MQ=60)
                                                                                      AACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAG        >  SRR4302535.2482037/1‑94 (MQ=60)
                                                                                       ACTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGC       >  SRR4302535.152196/1‑94 (MQ=60)
                                                                                        CTAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCT      <  SRR4302535.4060721/94‑1 (MQ=60)
                                                                                         TAATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCACACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTG     >  SRR4302535.3919117/1‑94 (MQ=60)
                                                                                          AATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGG    >  SRR4302535.1120120/1‑94 (MQ=60)
                                                                                          AATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGG    >  SRR4302535.3715320/1‑94 (MQ=60)
                                                                                          AATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGG    >  SRR4302535.2085557/1‑94 (MQ=60)
                                                                                           ATGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGC   >  SRR4302535.1303771/1‑94 (MQ=60)
                                                                                            TGCCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCA  <  SRR4302535.842320/94‑1 (MQ=60)
                                                                                             |                                                                                            
TTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGAACTAATACCGCCAGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCA  >  NC_000913/4414374‑4414559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑