Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,450,334 G→A A64T (GCT→ACT)  ytfT → putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,450,3340GA98.9% 634.6 / NA 188A64T (GCT→ACT) ytfTputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (88/97);  total (88/100)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

TCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCC  >  NC_000913/4450245‑4450423
                                                                                         |                                                                                         
tCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTc                                                                                        <  1:2007762/93‑1 (MQ=255)
 cGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTcc                                                                                       <  1:1013664/93‑1 (MQ=255)
  gATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTccc                                                                                      >  1:4202193/1‑93 (MQ=255)
   aTAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCg                                                                                     >  2:3922827/1‑93 (MQ=255)
    tAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGt                                                                                    >  1:148151/1‑93 (MQ=255)
     aGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGtt                                                                                   <  2:3574928/93‑1 (MQ=255)
     aGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGtt                                                                                   <  1:2871127/93‑1 (MQ=255)
     aGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGtt                                                                                   <  2:4640116/93‑1 (MQ=255)
     aGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGCTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGtt                                                                                   >  2:3631381/1‑93 (MQ=255)
      gCCTGGTAGCGCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTg                                                                                  <  1:3827821/93‑1 (MQ=255)
      gCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTg                                                                                  >  1:2380518/1‑93 (MQ=255)
       ccTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGc                                                                                 >  1:462368/1‑93 (MQ=255)
         tGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAGCCGTGCAACTCCCGTTGCGt                                                                               <  1:349386/93‑1 (MQ=255)
         tGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGt                                                                               <  1:230283/93‑1 (MQ=255)
            tAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAAGTCCCGTTGCGTTat                                                                            >  1:1792015/1‑92 (MQ=255)
            tAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTGCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTAc                                                                            <  2:2818475/93‑1 (MQ=255)
             aGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACt                                                                           <  2:168205/93‑1 (MQ=255)
              gCCGCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTg                                                                          <  1:4017783/93‑1 (MQ=255)
              gCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTg                                                                          <  1:1759005/93‑1 (MQ=255)
              gCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTg                                                                          >  2:3428343/1‑93 (MQ=255)
              gCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTg                                                                          >  1:1960132/1‑93 (MQ=255)
              gCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTg                                                                          <  2:4525944/93‑1 (MQ=255)
              gCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTg                                                                          <  2:4565072/93‑1 (MQ=255)
               ccccGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTgg                                                                         <  1:4497368/93‑1 (MQ=255)
                cccGCATTTCTGGCAGGGGGTGCTTCAGGCTGGGCGTTTGTTCGGTAGCCCCCTAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGc                                                                        >  2:4069144/1‑93 (MQ=255)
                  cGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGa                                                                      >  2:1100617/1‑93 (MQ=255)
                  cGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGa                                                                      >  1:1435748/1‑93 (MQ=255)
                   gCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAt                                                                     <  1:2551965/93‑1 (MQ=255)
                   gCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAt                                                                     >  2:4669545/1‑93 (MQ=255)
                     aTTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTACCCGTGCAACTCCCGTTGCGTTACTGGCGATTg                                                                   <  1:2515547/93‑1 (MQ=255)
                     aTTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTg                                                                   <  1:556563/93‑1 (MQ=255)
                     aTTTCTGGCAGGTGGGGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAGCCGTGCAGCTCCCGTTGCGTTACTGGCGATTg                                                                   <  2:559098/93‑1 (MQ=255)
                           ggCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGa                                                             <  2:1554186/93‑1 (MQ=255)
                            gCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAc                                                            >  1:2740225/1‑93 (MQ=255)
                              aGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGc                                                          >  2:2586443/1‑93 (MQ=255)
                               ggtggtGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCt                                                         <  2:592939/93‑1 (MQ=255)
                                gtggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTg                                                        >  1:3166389/1‑93 (MQ=255)
                                 tggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACTTTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTgg                                                       <  1:2145086/93‑1 (MQ=255)
                                 tggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTgg                                                       >  2:3660028/1‑93 (MQ=255)
                                  gttgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGt                                                      <  2:86053/91‑1 (MQ=255)
                                  ggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGt                                                      <  1:710313/93‑1 (MQ=255)
                                  ggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGt                                                      <  2:1805168/93‑1 (MQ=255)
                                  ggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGt                                                      >  2:224972/1‑93 (MQ=255)
                                  ggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGAGACTGGCATGACGCGGGt                                                      >  1:297655/1‑93 (MQ=255)
                                   gtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTg                                                     <  2:3145648/93‑1 (MQ=255)
                                     gCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAt                                                   <  2:817616/93‑1 (MQ=255)
                                          aGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCa                                              <  1:1175927/93‑1 (MQ=255)
                                          aGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCa                                              >  1:3660428/1‑93 (MQ=255)
                                           ggATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCAc                                             <  1:2347635/93‑1 (MQ=255)
                                           ggATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCAc                                             >  2:3761557/1‑93 (MQ=255)
                                            gATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTACCCGTGCAACTCCCGTTGCGTTCCTGGCGATTGGCATGACGCTGGTGATCGCCAcc                                            <  2:1450152/93‑1 (MQ=255)
                                             aTGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCg                                           >  2:1385913/1‑93 (MQ=255)
                                              tGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCgg                                          >  2:2442031/1‑93 (MQ=255)
                                              tGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCgg                                          >  1:3271636/1‑93 (MQ=255)
                                              gtgtcGTTTGTTCGGTAGCCCCATAGACATTCTTATCCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCgg                                          <  1:260259/89‑1 (MQ=255)
                                               gggCGTTTGTTCGGTAGCCCCATAGACATTCTTACCCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGt                                         <  2:2530179/93‑1 (MQ=255)
                                                ggCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTg                                        <  2:328198/93‑1 (MQ=255)
                                                ggCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTg                                        >  2:128121/1‑93 (MQ=255)
                                                 gCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTgg                                       <  2:1766331/93‑1 (MQ=255)
                                                 gCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTgg                                       <  1:585524/93‑1 (MQ=255)
                                                 gCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTgg                                       <  1:3190322/93‑1 (MQ=255)
                                                  cGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTggg                                      >  2:2333529/1‑93 (MQ=255)
                                                  cGTTTGTTCGGTAGCCCCATAGACATCCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTggg                                      <  2:2476877/93‑1 (MQ=255)
                                                   gTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGa                                     <  1:3916761/93‑1 (MQ=255)
                                                   gTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGa                                     <  1:2124765/93‑1 (MQ=255)
                                                   gTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGa                                     <  2:4211414/93‑1 (MQ=255)
                                                    tttGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAt                                    >  1:305603/1‑93 (MQ=255)
                                                    tttGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAt                                    >  1:3340324/1‑93 (MQ=255)
                                                    tttGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAt                                    >  2:1879721/1‑93 (MQ=255)
                                                       gTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGa                                 <  1:1230919/93‑1 (MQ=255)
                                                       gTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGa                                 >  1:3617118/1‑93 (MQ=255)
                                                          cGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtct                              >  1:939954/1‑93 (MQ=255)
                                                            gTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtctct                            >  1:4536881/1‑93 (MQ=255)
                                                             tAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtctctc                           <  1:2234/93‑1 (MQ=255)
                                                             tAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtctctc                           <  1:1515775/93‑1 (MQ=255)
                                                              aGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTcc                          <  1:4365385/93‑1 (MQ=255)
                                                               gCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCg                         >  1:4643162/1‑93 (MQ=255)
                                                                 cccATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGTATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTa                       >  1:1723304/1‑93 (MQ=255)
                                                                 cccATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTa                       <  1:987654/93‑1 (MQ=255)
                                                                  ccATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAg                      >  2:2251774/1‑93 (MQ=255)
                                                                  ccATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAg                      >  2:1170140/1‑93 (MQ=255)
                                                                   cATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgg                     <  1:3642294/93‑1 (MQ=255)
                                                                   cATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgg                     >  1:166613/1‑93 (MQ=255)
                                                                    aTAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAggg                    >  1:1269659/1‑93 (MQ=255)
                                                                    aTAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAggg                    <  1:1860953/93‑1 (MQ=255)
                                                                     tAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgggg                   >  1:1030389/1‑93 (MQ=255)
                                                                     tAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgggg                   >  1:839835/1‑93 (MQ=255)
                                                                     tAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgggg                   >  2:174314/1‑93 (MQ=255)
                                                                       gACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCg                 >  2:2061575/1‑93 (MQ=255)
                                                                        aCATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGGGGGATTGATCTCTCCGTAGGGGCgg                >  2:3729091/1‑93 (MQ=255)
                                                                          aTTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTg              >  2:1500789/1‑93 (MQ=255)
                                                                           ttCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGa             >  2:2162112/1‑93 (MQ=255)
                                                                            tCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGAt            <  2:3953850/93‑1 (MQ=255)
                                                                             cTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATg           <  2:2725649/93‑1 (MQ=255)
                                                                               tAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGc         <  1:1609263/93‑1 (MQ=255)
                                                                                aaCCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCt        >  1:627055/1‑93 (MQ=255)
                                                                                 aCCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTa       >  1:484509/1‑93 (MQ=255)
                                                                                   cGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATc     >  1:3529851/1‑93 (MQ=255)
                                                                                     tGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGc   >  1:669649/1‑93 (MQ=255)
                                                                                      gCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGcc  >  1:2613796/1‑93 (MQ=255)
                                                                                         |                                                                                         
TCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCC  >  NC_000913/4450245‑4450423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGA  >  NC_000913/4450241‑4450426
                                                                                             |                                                                                            
CTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAA                                                                                              >  SRR4302535.2899007/1‑94 (MQ=60)
 TGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAAC                                                                                             <  SRR4302535.3164602/94‑1 (MQ=60)
    TCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCC                                                                                          <  SRR4302535.2022224/94‑1 (MQ=60)
     CGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCC                                                                                         <  SRR4302535.1020871/94‑1 (MQ=60)
     CGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCC                                                                                         >  SRR4302535.4230943/1‑94 (MQ=60)
     CGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCC                                                                                         <  SRR4302535.959871/94‑1 (MQ=60)
      GATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCG                                                                                        >  SRR4302535.656623/1‑94 (MQ=60)
       ATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGT                                                                                       <  SRR4302535.594741/94‑1 (MQ=60)
       ATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGT                                                                                       >  SRR4302535.149184/1‑94 (MQ=60)
         AGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTG                                                                                     <  SRR4302535.2891469/94‑1 (MQ=60)
         AGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTG                                                                                     >  SRR4302535.224512/1‑94 (MQ=60)
         AGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTG                                                                                     >  SRR4302535.2397615/1‑94 (MQ=60)
          GCCTGGTAGCGCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGC                                                                                    <  SRR4302535.3854224/94‑1 (MQ=60)
          GCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGC                                                                                    >  SRR4302535.465596/1‑94 (MQ=60)
            CTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGT                                                                                  <  SRR4302535.3142994/94‑1 (MQ=60)
             TGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAGCCGTGCAACTCCCGTTGCGTT                                                                                 <  SRR4302535.351790/94‑1 (MQ=60)
             TGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTT                                                                                 <  SRR4302535.231871/94‑1 (MQ=60)
              GGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTA                                                                                <  SRR4302535.2588797/94‑1 (MQ=60)
              GGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTA                                                                                >  SRR4302535.2295231/1‑94 (MQ=60)
               GTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAAGTCCCGTTGCGTTAT                                                                               >  SRR4302535.1804971/1‑94 (MQ=60)
               GTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTAC                                                                               >  SRR4302535.176336/1‑94 (MQ=60)
                 AGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTG                                                                             >  SRR4302535.1256078/1‑94 (MQ=60)
                 AGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTG                                                                             >  SRR4302535.1974271/1‑94 (MQ=60)
                  GCCGCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGG                                                                            <  SRR4302535.4045268/94‑1 (MQ=60)
                  GCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGG                                                                            <  SRR4302535.4086468/94‑1 (MQ=60)
                  GCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGG                                                                            <  SRR4302535.1771741/94‑1 (MQ=60)
                   CCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGC                                                                           <  SRR4302535.4528246/94‑1 (MQ=60)
                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          >  SRR4302535.567786/1‑94 (MQ=60)
                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          >  SRR4302535.4531043/1‑94 (MQ=60)
                     CCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGA                                                                         >  SRR4302535.1446007/1‑94 (MQ=60)
                     CCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGA                                                                         >  SRR4302535.3244900/1‑94 (MQ=60)
                       GCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATT                                                                       >  SRR4302535.712589/1‑94 (MQ=60)
                       GCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATT                                                                       <  SRR4302535.2570339/94‑1 (MQ=60)
                       GCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATT                                                                       <  SRR4302535.3206553/94‑1 (MQ=60)
                       GCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATT                                                                       >  SRR4302535.55482/1‑94 (MQ=60)
                        CATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTG                                                                      >  SRR4302535.2047377/1‑94 (MQ=60)
                        CATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTG                                                                      >  SRR4302535.1919418/1‑94 (MQ=60)
                        CATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTG                                                                      >  SRR4302535.281262/1‑94 (MQ=60)
                         ATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTACCCGTGCAACTCCCGTTGCGTTACTGGCGATTGG                                                                     <  SRR4302535.2533679/94‑1 (MQ=60)
                         ATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGG                                                                     <  SRR4302535.560464/94‑1 (MQ=60)
                            TCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCAT                                                                  <  SRR4302535.3446922/94‑1 (MQ=60)
                              TGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGA                                                                >  SRR4302535.3953407/1‑94 (MQ=60)
                               GGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAC                                                               >  SRR4302535.2759758/1‑94 (MQ=60)
                                  AGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCACCTCCCGTTGCGTTACTGGCGATTGGCATGACGCT                                                            <  SRR4302535.1734172/94‑1 (MQ=60)
                                  AGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCT                                                            <  SRR4302535.1212367/94‑1 (MQ=60)
                                  AGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCT                                                            <  SRR4302535.3643373/94‑1 (MQ=60)
                                   GGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTG                                                           >  SRR4302535.3188332/1‑94 (MQ=60)
                                    GTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGG                                                          <  SRR4302535.1080144/94‑1 (MQ=60)
                                     TGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACTTTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGT                                                         <  SRR4302535.2160518/94‑1 (MQ=60)
                                     TGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGT                                                         <  SRR4302535.479144/94‑1 (MQ=60)
                                     TGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGAGACTGGCATGACGCGGGT                                                         >  SRR4302535.299729/1‑94 (MQ=60)
                                      GGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTG                                                        <  SRR4302535.715298/94‑1 (MQ=60)
                                      GGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTG                                                        <  SRR4302535.368004/94‑1 (MQ=60)
                                       GTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGA                                                       <  SRR4302535.4605686/94‑1 (MQ=60)
                                          CTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCG                                                    <  SRR4302535.2918101/94‑1 (MQ=60)
                                             CAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCA                                                 >  SRR4302535.3685764/1‑94 (MQ=60)
                                              AGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCAC                                                <  SRR4302535.1184322/94‑1 (MQ=60)
                                               GGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACC                                               <  SRR4302535.2364462/94‑1 (MQ=60)
                                                 ATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGG                                             >  SRR4302535.3294364/1‑94 (MQ=60)
                                                  gtgtCGTTTGTTCGGTAGCCCCATAGACATTCTTATCCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGT                                            <  SRR4302535.262064/90‑1 (MQ=60)
                                                  TGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGT                                            >  SRR4302535.2158122/1‑94 (MQ=60)
                                                     GCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGG                                         <  SRR4302535.589628/94‑1 (MQ=60)
                                                     GCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGG                                         <  SRR4302535.3212385/94‑1 (MQ=60)
                                                       GTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAT                                       <  SRR4302535.3943563/94‑1 (MQ=60)
                                                       GTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAT                                       >  SRR4302535.307721/1‑94 (MQ=60)
                                                       GTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAT                                       <  SRR4302535.2140077/94‑1 (MQ=60)
                                                       GTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAT                                       >  SRR4302535.3363600/1‑94 (MQ=60)
                                                          TGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGA                                    >  SRR4302535.3642196/1‑94 (MQ=60)
                                                          TGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGA                                    >  SRR4302535.3521864/1‑94 (MQ=60)
                                                           GTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAT                                   <  SRR4302535.1239720/94‑1 (MQ=60)
                                                           GTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAT                                   <  SRR4302535.1412774/94‑1 (MQ=60)
                                                             TCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCT                                 >  SRR4302535.946610/1‑94 (MQ=60)
                                                             TCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCT                                 <  SRR4302535.2565655/94‑1 (MQ=60)
                                                             TCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCT                                 <  SRR4302535.2341115/94‑1 (MQ=60)
                                                               GGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCT                               >  SRR4302535.4567918/1‑94 (MQ=60)
                                                               GGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCT                               <  SRR4302535.1505458/94‑1 (MQ=60)
                                                                GTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTC                              <  SRR4302535.3311584/94‑1 (MQ=60)
                                                                 TAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCC                             <  SRR4302535.1526694/94‑1 (MQ=60)
                                                                 TAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCC                             <  SRR4302535.2254/94‑1 (MQ=60)
                                                                  AGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCG                            <  SRR4302535.1839987/94‑1 (MQ=60)
                                                                  AGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCG                            <  SRR4302535.4395257/94‑1 (MQ=60)
                                                                  AGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCG                            <  SRR4302535.2750175/94‑1 (MQ=60)
                                                                  AGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCG                            >  SRR4302535.4674632/1‑94 (MQ=60)
                                                                    CCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGTATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTA                          >  SRR4302535.1735766/1‑94 (MQ=60)
                                                                     CCCATAGACATTCTTAACCGTGCAACTCCCGTTTCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAGTGATCTCTCCGTAG                         >  SRR4302535.3855973/1‑94 (MQ=60)
                                                                     CCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAG                         <  SRR4302535.994670/94‑1 (MQ=60)
                                                                      CCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGG                        <  SRR4302535.3575634/94‑1 (MQ=60)
                                                                      CCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGG                        <  SRR4302535.762380/94‑1 (MQ=60)
                                                                      CCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGG                        >  SRR4302535.167772/1‑94 (MQ=60)
                                                                       CATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGG                       <  SRR4302535.3667520/94‑1 (MQ=60)
                                                                       CATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGG                       >  SRR4302535.1278733/1‑94 (MQ=60)
                                                                       CATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGG                       <  SRR4302535.1561274/94‑1 (MQ=60)
                                                                       CATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGG                       >  SRR4302535.2509966/1‑94 (MQ=60)
                                                                        ATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGG                      >  SRR4302535.1037705/1‑94 (MQ=60)
                                                                        ATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGG                      >  SRR4302535.845733/1‑94 (MQ=60)
                                                                        ATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGG                      >  SRR4302535.3766940/1‑94 (MQ=60)
                                                                        ATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGG                      <  SRR4302535.1874372/94‑1 (MQ=60)
                                                                           GACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGG                   >  SRR4302535.1662615/1‑94 (MQ=60)
                                                                             CATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTG                 >  SRR4302535.2622639/1‑94 (MQ=60)
                                                                              ATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGA                >  SRR4302535.881888/1‑94 (MQ=60)
                                                                                TCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGCGATG              >  SRR4302535.1465615/1‑94 (MQ=60)
                                                                                   TAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCT           >  SRR4302535.631422/1‑94 (MQ=60)
                                                                                   TAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCT           <  SRR4302535.1620867/94‑1 (MQ=60)
                                                                                    AACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTA          >  SRR4302535.487905/1‑94 (MQ=60)
                                                                                     ACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTAT         <  SRR4302535.2375135/94‑1 (MQ=60)
                                                                                      CCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATC        >  SRR4302535.3554360/1‑94 (MQ=60)
                                                                                       CGTGCACCTCCCGTTGTGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAGCTCTCCGTAGGGGCGGTGATGGCTATCG       <  SRR4302535.4399798/94‑1 (MQ=60)
                                                                                       CGTGCACCTCCCGTTGCTTTACTGGCGATTGGCATGACGCTGGTGATCGCCCCGGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCG       <  SRR4302535.1662256/94‑1 (MQ=60)
                                                                                       CGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCG       <  SRR4302535.4665548/94‑1 (MQ=60)
                                                                                        GTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGC      >  SRR4302535.180116/1‑94 (MQ=60)
                                                                                        GTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGC      >  SRR4302535.674323/1‑94 (MQ=60)
                                                                                         TGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCC     >  SRR4302535.2632541/1‑94 (MQ=60)
                                                                                          GCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCG    >  SRR4302535.4323251/1‑94 (MQ=60)
                                                                                          GCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCG    >  SRR4302535.699927/1‑94 (MQ=60)
                                                                                           CAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGG   <  SRR4302535.3631340/94‑1 (MQ=60)
                                                                                           CAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGG   <  SRR4302535.741675/94‑1 (MQ=60)
                                                                                           CAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGG   <  SRR4302535.1620224/94‑1 (MQ=60)
                                                                                            AACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGA  >  SRR4302535.762019/1‑94 (MQ=60)
                                                                                             |                                                                                            
CTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGA  >  NC_000913/4450241‑4450426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑