Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,662	A→G	G71G (GGT→GGC)	argI ←	ornithine carbamoyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,662	0	A	G	99.6%	772.7 / ‑5.4	249	G71G (GGT→GGC)	argI	ornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (122/126); total (122/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 255 total aligned reads displayed.

ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTG  >  NC_000913/4475572‑4475752

aTACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGcc                                                                                          >  1:1049099/1‑93 (MQ=255)

 tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGccc                                                                                         <  1:1086311/93‑1 (MQ=255)

 tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGccc                                                                                         >  1:1151327/1‑93 (MQ=255)

  aCATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGCCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCt                                                                                        <  1:203927/93‑1 (MQ=255)

   cATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTg                                                                                       <  2:3262502/93‑1 (MQ=255)

   cATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTg                                                                                       >  2:1747382/1‑93 (MQ=255)

   cATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTg                                                                                       >  1:596431/1‑93 (MQ=255)

    aTGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTgg                                                                                      >  2:3635609/1‑93 (MQ=255)

    aTGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTgg                                                                                      >  2:3112493/1‑93 (MQ=255)

     tGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGt                                                                                     >  1:2254400/1‑93 (MQ=255)

      gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTc                                                                                    >  1:3690545/1‑93 (MQ=255)

        gACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCat                                                                                  >  2:4025545/1‑93 (MQ=255)

        gACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCat                                                                                  >  1:4275088/1‑93 (MQ=255)

         aCCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCGTGGTCata                                                                                 >  1:971728/1‑93 (MQ=255)

         aCCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCata                                                                                 <  1:434123/93‑1 (MQ=255)

          ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCatat                                                                                <  2:2848129/93‑1 (MQ=255)

           cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATg                                                                               <  1:708056/93‑1 (MQ=255)

             aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCg                                                                             >  2:480583/1‑93 (MQ=255)

             aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCg                                                                             >  2:454975/1‑93 (MQ=255)

             aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCg                                                                             <  1:4248324/93‑1 (MQ=255)

                 cgcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCaa                                                                         <  1:4435681/93‑1 (MQ=255)

                  gcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAAc                                                                        <  2:1995556/93‑1 (MQ=255)

                   cgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACt                                                                       <  2:3982238/93‑1 (MQ=255)

                     ggCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTc                                                                     <  1:2869888/93‑1 (MQ=255)

                     ggCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTc                                                                     <  1:39615/93‑1 (MQ=255)

                      gCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCg                                                                    <  2:4320660/93‑1 (MQ=255)

                        aGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGaa                                                                  <  1:2277179/93‑1 (MQ=255)

                        aGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGaa                                                                  >  2:2603760/1‑93 (MQ=255)

                        aGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGaa                                                                  <  2:3103244/93‑1 (MQ=255)

                         gtgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGaaa                                                                 >  1:3287732/1‑93 (MQ=255)

                           gtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAaga                                                               >  2:1203160/1‑93 (MQ=255)

                            tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATACGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAagag                                                              <  1:2517006/93‑1 (MQ=255)

                            tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCGACTTCGAAagag                                                              >  2:2795916/1‑93 (MQ=255)

                            tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAagag                                                              >  2:1897393/1‑93 (MQ=255)

                             cTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGc                                                             >  2:2518308/1‑93 (MQ=255)

                             cTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGc                                                             >  1:3156017/1‑93 (MQ=255)

                             cTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGc                                                             >  2:1480525/1‑93 (MQ=255)

                              tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCa                                                            <  2:3539004/93‑1 (MQ=255)

                              tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCCACAGAGCa                                                            >  2:360585/1‑93 (MQ=255)

                               ttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCAt                                                           <  2:2070454/93‑1 (MQ=255)

                               ttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCAt                                                           >  2:115162/1‑93 (MQ=255)

                               ttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCAt                                                           >  2:1071465/1‑93 (MQ=255)

                               ttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCAt                                                           <  2:3720284/93‑1 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                          >  2:1814038/1‑93 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                          >  2:433726/1‑93 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                          <  2:4375977/93‑1 (MQ=255)

                                  aTCGCCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCgg                                                        <  1:4384668/93‑1 (MQ=255)

                                  aTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCgg                                                        >  1:1788331/1‑93 (MQ=255)

                                   tCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg                                                       >  2:2716486/1‑93 (MQ=255)

                                     gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTa                                                     <  1:3658465/93‑1 (MQ=255)

                                      aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc                                                    <  1:3816427/93‑1 (MQ=255)

                                       ctctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcg                                                   >  1:3231888/1‑93 (MQ=255)

                                       ctctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcg                                                   >  2:2360028/1‑93 (MQ=255)

                                        tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgt                                                  >  1:2828220/1‑92 (MQ=255)

                                        tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga                                                  <  1:1224023/93‑1 (MQ=255)

                                        tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga                                                  <  1:4123612/93‑1 (MQ=255)

                                          tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt                                                <  2:3449516/93‑1 (MQ=255)

                                          tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt                                                <  1:561444/93‑1 (MQ=255)

                                           ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc                                               <  2:1686342/93‑1 (MQ=255)

                                            tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcg                                              >  2:300216/1‑93 (MQ=255)

                                               gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcgagt                                           >  2:1953510/1‑93 (MQ=255)

                                               gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcgagt                                           >  1:3545833/1‑93 (MQ=255)

                                                 ccAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt                                         >  1:1894820/1‑93 (MQ=255)

                                                 ccAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt                                         <  2:2578077/93‑1 (MQ=255)

                                                 ccAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt                                         <  1:4627614/93‑1 (MQ=255)

                                                  cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtt                                        >  1:1476443/1‑93 (MQ=255)

                                                  cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtt                                        <  1:2433331/93‑1 (MQ=255)

                                                  cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtt                                        >  1:3033081/1‑93 (MQ=255)

                                                   aaTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt                                       >  1:4616494/1‑93 (MQ=255)

                                                   aaTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt                                       >  2:2966443/1‑93 (MQ=255)

                                                    aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt                                      <  2:2182125/93‑1 (MQ=255)

                                                    aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt                                      <  2:95472/93‑1 (MQ=255)

                                                    aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt                                      >  1:1703683/1‑93 (MQ=255)

                                                     tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttttt                                     <  2:4003605/93‑1 (MQ=255)

                                                     tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttttt                                     >  1:1546524/1‑93 (MQ=255)

                                                     tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttttt                                     >  1:4028489/1‑93 (MQ=255)

                                                        ggCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGa                                  >  1:2477496/1‑93 (MQ=255)

                                                          cTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAGATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAg                                >  1:2518663/1‑93 (MQ=255)

                                                           tGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGa                               >  2:866480/1‑93 (MQ=255)

                                                           tGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGa                               >  1:1666297/1‑93 (MQ=255)

                                                              cGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa                            <  2:468958/93‑1 (MQ=255)

                                                              cGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa                            <  2:3771148/93‑1 (MQ=255)

                                                                 ttGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAgcg                         <  1:3910607/93‑1 (MQ=255)

                                                                 ttGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAgcg                         >  1:3432300/1‑93 (MQ=255)

                                                                 ttGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAgcg                         >  1:1755455/1‑93 (MQ=255)

                                                                   gggCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGGCTTTTTCGAAGATGAGcgcg                       >  2:256407/1‑93 (MQ=255)

                                                                     gCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAt                     <  2:3859350/93‑1 (MQ=255)

                                                                     gCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAt                     >  1:2633337/1‑93 (MQ=255)

                                                                     gCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAt                     >  1:2787937/1‑93 (MQ=255)

                                                                       cGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt                   <  2:606274/93‑1 (MQ=255)

                                                                       cGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt                   >  1:3082972/1‑93 (MQ=255)

                                                                       cGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt                   <  2:3356737/93‑1 (MQ=255)

                                                                          gaTAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtttt                <  1:2130808/93‑1 (MQ=255)

                                                                                   cgcgAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGa       <  2:4176399/93‑1 (MQ=255)

                                                                                     cgAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGt     <  1:936473/93‑1 (MQ=255)

                                                                                      gAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGCACATGAGCGCGATGTTTTTACCAGTGAGtt    >  1:2786129/1‑93 (MQ=255)

                                                                                      gAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGtt    <  2:4113904/93‑1 (MQ=255)

                                                                                      gAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGtt    <  1:4484457/93‑1 (MQ=255)

                                                                                      gAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGtt    <  2:1341859/93‑1 (MQ=255)

                                                                                      gAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGtt    <  2:1268301/93‑1 (MQ=255)

ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTG  >  NC_000913/4475572‑4475752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCT  >  NC_000913/4475569‑4475755

GTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCG                                                                                               >  SRR4302535.3587076/1‑94 (MQ=60)

  CATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCC                                                                                             >  SRR4302535.1056562/1‑94 (MQ=60)

  CATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCC                                                                                             >  SRR4302535.3699467/1‑94 (MQ=60)

   ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCC                                                                                            >  SRR4302535.2542970/1‑94 (MQ=60)

   ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCC                                                                                            >  SRR4302535.1159550/1‑94 (MQ=60)

   ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCC                                                                                            >  SRR4302535.2630195/1‑94 (MQ=60)

   ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCC                                                                                            >  SRR4302535.4226448/1‑94 (MQ=60)

    TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCT                                                                                           <  SRR4302535.1094033/94‑1 (MQ=60)

     ACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGCCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTG                                                                                          <  SRR4302535.205323/94‑1 (MQ=60)

     ACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTG                                                                                          <  SRR4302535.671951/94‑1 (MQ=60)

     ACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTG                                                                                          >  SRR4302535.600611/1‑94 (MQ=60)

      CATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGG                                                                                         <  SRR4302535.3566219/94‑1 (MQ=60)

       ATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGT                                                                                        >  SRR4302535.2270452/1‑94 (MQ=60)

        TGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTC                                                                                       >  SRR4302535.3716088/1‑94 (MQ=60)

          CGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAT                                                                                     <  SRR4302535.3924294/94‑1 (MQ=60)

          CGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAT                                                                                     >  SRR4302535.4078903/1‑94 (MQ=60)

          CGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAT                                                                                     >  SRR4302535.4304351/1‑94 (MQ=60)

           GACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCGTGGTCATA                                                                                    >  SRR4302535.978623/1‑94 (MQ=60)

           GACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATA                                                                                    >  SRR4302535.2496758/1‑94 (MQ=60)

            ACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATAT                                                                                   <  SRR4302535.437146/94‑1 (MQ=60)

            ACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATAT                                                                                   <  SRR4302535.290569/94‑1 (MQ=60)

             CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATG                                                                                  >  SRR4302535.1068978/1‑94 (MQ=60)

             CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATG                                                                                  >  SRR4302535.2114523/1‑94 (MQ=60)

             CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATG                                                                                  >  SRR4302535.2036961/1‑94 (MQ=60)

             CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATG                                                                                  <  SRR4302535.4453693/94‑1 (MQ=60)

             CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATG                                                                                  >  SRR4302535.362324/1‑94 (MQ=60)

              CAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGC                                                                                 <  SRR4302535.713024/94‑1 (MQ=60)

                AGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGG                                                                               <  SRR4302535.4309364/94‑1 (MQ=60)

                AGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGG                                                                               <  SRR4302535.4277419/94‑1 (MQ=60)

                  CACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCA                                                                             >  SRR4302535.4651139/1‑94 (MQ=60)

                   ACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAA                                                                            >  SRR4302535.2181467/1‑94 (MQ=60)

                    CGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAAC                                                                           <  SRR4302535.4466081/94‑1 (MQ=60)

                     GCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACT                                                                          <  SRR4302535.3096992/94‑1 (MQ=60)

                     GCGGGCAGTGTCTCTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACT                                                                          <  SRR4302535.3159455/94‑1 (MQ=60)

                     GCGGGCAGGGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACT                                                                          <  SRR4302535.4334848/94‑1 (MQ=60)

                      CGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTT                                                                         <  SRR4302535.3140203/94‑1 (MQ=60)

                      CGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTT                                                                         <  SRR4302535.2519969/94‑1 (MQ=60)

                       GGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTC                                                                        <  SRR4302535.4286540/94‑1 (MQ=60)

                        GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCG                                                                       >  SRR4302535.1973069/1‑94 (MQ=60)

                        GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCG                                                                       <  SRR4302535.2890217/94‑1 (MQ=60)

                        GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCG                                                                       <  SRR4302535.509589/94‑1 (MQ=60)

                        GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCG                                                                       <  SRR4302535.39918/94‑1 (MQ=60)

                         GCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGGGGCAACTTCGA                                                                      >  SRR4302535.647667/1‑94 (MQ=60)

                         GCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGA                                                                      <  SRR4302535.1884770/94‑1 (MQ=60)

                           AGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAA                                                                    >  SRR4302535.1685351/1‑94 (MQ=60)

                           AGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAA                                                                    <  SRR4302535.2293440/94‑1 (MQ=60)

                           AGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAA                                                                    >  SRR4302535.3310589/1‑94 (MQ=60)

                            GTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCTGCAACTTCGAAAG                                                                   >  SRR4302535.3943608/1‑94 (MQ=60)

                             TGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGA                                                                  <  SRR4302535.1249647/94‑1 (MQ=60)

                             TGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGA                                                                  <  SRR4302535.3344860/94‑1 (MQ=60)

                               TCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATACGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGC                                                                <  SRR4302535.2535150/94‑1 (MQ=60)

                               TCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGC                                                                >  SRR4302535.950185/1‑94 (MQ=60)

                               TCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGC                                                                <  SRR4302535.4281760/94‑1 (MQ=60)

                               TCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGC                                                                <  SRR4302535.973679/94‑1 (MQ=60)

                               TCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGC                                                                >  SRR4302535.3177934/1‑94 (MQ=60)

                                CTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               <  SRR4302535.2434085/94‑1 (MQ=60)

                                CTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               <  SRR4302535.4484874/94‑1 (MQ=60)

                                CTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               >  SRR4302535.1716467/1‑94 (MQ=60)

                                   TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCG                                                            <  SRR4302535.3683000/94‑1 (MQ=60)

                                    AATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGG                                                           >  SRR4302535.1801270/1‑94 (MQ=60)

                                    AATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGG                                                           >  SRR4302535.1101027/1‑94 (MQ=60)

                                     ATCGCCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGG                                                          <  SRR4302535.4414668/94‑1 (MQ=60)

                                     ATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGG                                                          >  SRR4302535.511872/1‑94 (MQ=60)

                                        GACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAC                                                       <  SRR4302535.3683790/94‑1 (MQ=60)

                                         ACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACG                                                      <  SRR4302535.3842761/94‑1 (MQ=60)

                                         ACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACG                                                      >  SRR4302535.3254299/1‑94 (MQ=60)

                                          CTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGT                                                     >  SRR4302535.2848292/1‑94 (MQ=60)

                                          CTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGA                                                     >  SRR4302535.4087089/1‑94 (MQ=60)

                                           TCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAG                                                    <  SRR4302535.4151831/94‑1 (MQ=60)

                                           TCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAG                                                    <  SRR4302535.1232779/94‑1 (MQ=60)

                                           TCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAG                                                    >  SRR4302535.3528130/1‑94 (MQ=60)

                                           CCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAG                                                    <  SRR4302535.1509262/94‑1 (MQ=60)

                                            TTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGT                                                   <  SRR4302535.4008834/94‑1 (MQ=60)

                                             TTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTC                                                  <  SRR4302535.565369/94‑1 (MQ=60)

                                             TTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTC                                                  >  SRR4302535.2464445/1‑94 (MQ=60)

                                              TTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCTAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCG                                                 >  SRR4302535.2535847/1‑94 (MQ=60)

                                                ATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAG                                               >  SRR4302535.4403239/1‑94 (MQ=60)

                                                ATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAG                                               >  SRR4302535.16057/1‑94 (MQ=60)

                                                 TGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGT                                              >  SRR4302535.3570454/1‑94 (MQ=60)

                                                   ACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT                                            >  SRR4302535.1908457/1‑94 (MQ=60)

                                                    CCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTT                                           >  SRR4302535.1487011/1‑94 (MQ=60)

                                                    CCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTT                                           <  SRR4302535.4659027/94‑1 (MQ=60)

                                                    CCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTT                                           >  SRR4302535.3796382/1‑94 (MQ=60)

                                                    CCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTT                                           >  SRR4302535.3054313/1‑94 (MQ=60)

                                                     CAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTT                                          >  SRR4302535.4647868/1‑94 (MQ=60)

                                                     CAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTT                                          <  SRR4302535.2450863/94‑1 (MQ=60)

                                                      AATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTT                                         >  SRR4302535.1716000/1‑94 (MQ=60)

                                                       ATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTT                                        >  SRR4302535.1557664/1‑94 (MQ=60)

                                                       ATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTT                                        >  SRR4302535.4056051/1‑94 (MQ=60)

                                                        TCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTC                                       <  SRR4302535.1908188/94‑1 (MQ=60)

                                                          TGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGA                                     >  SRR4302535.2495363/1‑94 (MQ=60)

                                                            GCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAGATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAG                                   >  SRR4302535.2536817/1‑94 (MQ=60)

                                                             CTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGA                                  >  SRR4302535.57666/1‑94 (MQ=60)

                                                             CTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGA                                  >  SRR4302535.1678352/1‑94 (MQ=60)

                                                              TGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAT                                 <  SRR4302535.3741449/94‑1 (MQ=60)

                                                               GCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTTGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATG                                >  SRR4302535.3246164/1‑94 (MQ=60)

                                                                 CGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAG                              <  SRR4302535.3955407/94‑1 (MQ=60)

                                                                 CGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAG                              <  SRR4302535.2811529/94‑1 (MQ=60)

                                                                  GCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGC                             >  SRR4302535.997542/1‑94 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            <  SRR4302535.1660164/94‑1 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            >  SRR4302535.2679387/1‑94 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            <  SRR4302535.2890668/94‑1 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            >  SRR4302535.3456152/1‑94 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            >  SRR4302535.1768140/1‑94 (MQ=60)

                                                                    TTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGC                           <  SRR4302535.3937383/94‑1 (MQ=60)

                                                                       GGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAT                        >  SRR4302535.2652206/1‑94 (MQ=53)

                                                                       GGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAT                        >  SRR4302535.2807776/1‑94 (MQ=53)

                                                                        GCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATG                       <  SRR4302535.1150930/94‑1 (MQ=50)

                                                                        GCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATG                       >  SRR4302535.4578826/1‑94 (MQ=50)

                                                                         CCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGT                      >  SRR4302535.1769085/1‑94 (MQ=48)

                                                                         CCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGT                      >  SRR4302535.3104456/1‑94 (MQ=48)

                                                                          CGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTT                     >  SRR4302535.1731605/1‑94 (MQ=45)

                                                                          CGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTT                     <  SRR4302535.744134/94‑1 (MQ=45)

                                                                          CGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTT                     >  SRR4302535.4603002/1‑94 (MQ=45)

                                                                           GAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTT                    <  SRR4302535.2269750/94‑1 (MQ=43)

                                                                           GAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTT                    >  SRR4302535.328833/1‑94 (MQ=43)

                                                                            AGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTT                   >  SRR4302535.3306254/1‑94 (MQ=40)

                                                                             GATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTT                  <  SRR4302535.2377237/94‑1 (MQ=38)

                                                                             GATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTT                  <  SRR4302535.2146171/94‑1 (MQ=38)

                                                                                 AGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCA              <  SRR4302535.1182650/94‑1 (MQ=30)

                                                                                  GTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAG             <  SRR4302535.2868251/94‑1 (MQ=30)

                                                                                    TACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTG           <  SRR4302535.2812455/94‑1 (MQ=35)

                                                                                      CGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAG         <  SRR4302535.2806851/94‑1 (MQ=40)

                                                                                        CGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGCACATGAGCGCGATGTTTTTACCAGTGAGTT       >  SRR4302535.2805957/1‑94 (MQ=37)

                                                                                        CGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTT       >  SRR4302535.2282493/1‑94 (MQ=40)

                                                                                        CGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTT       <  SRR4302535.943105/94‑1 (MQ=40)

                                                                                         GAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTT      <  SRR4302535.1345353/94‑1 (MQ=40)

                                                                                         GAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTT      <  SRR4302535.4438292/94‑1 (MQ=40)

                                                                                         GAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTT      <  SRR4302535.2804896/94‑1 (MQ=40)

                                                                                         GAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTT      <  SRR4302535.4515244/94‑1 (MQ=40)

                                                                                           GCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGG    <  SRR4302535.4301213/94‑1 (MQ=46)

                                                                                            CGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGC   >  SRR4302535.1640569/1‑94 (MQ=52)

                                                                                             GCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAATCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCT  >  SRR4302535.3328973/1‑94 (MQ=55)

GTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCT  >  NC_000913/4475569‑4475755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`