Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,662	A→G	G71G (GGT→GGC)	argI ←	ornithine carbamoyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,662	0	A	G	99.5%	1174.1 / ‑3.7	379	G71G (GGT→GGC)	argI	ornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (177/199); total (179/200)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

Only 100 of 388 total aligned reads displayed.

ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTT  >  NC_000913/4475572‑4475751

aTACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGcc                                                                                         <  1:2136312/93‑1 (MQ=255)

 tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGTCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGccc                                                                                        <  1:6009650/93‑1 (MQ=255)

 tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGccc                                                                                        <  1:3775402/93‑1 (MQ=255)

 tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGccc                                                                                        >  1:5624849/1‑93 (MQ=255)

 tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGccc                                                                                        <  1:6775198/93‑1 (MQ=255)

 tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGccc                                                                                        >  2:5130123/1‑93 (MQ=255)

 tACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGccc                                                                                        <  2:5543702/93‑1 (MQ=255)

  aCATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCt                                                                                       >  2:7808397/1‑93 (MQ=255)

  aCATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCt                                                                                       <  2:5751383/93‑1 (MQ=255)

  aCATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCt                                                                                       <  1:4399708/93‑1 (MQ=255)

    aTGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTgg                                                                                     >  1:1816066/1‑93 (MQ=255)

    aTGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTgg                                                                                     <  1:7408615/93‑1 (MQ=255)

     tGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGt                                                                                    >  1:5804199/1‑93 (MQ=255)

      gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTc                                                                                   <  1:2540091/93‑1 (MQ=255)

       cGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCa                                                                                  <  2:4123443/93‑1 (MQ=255)

        gACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCat                                                                                 >  2:5689756/1‑93 (MQ=255)

         aCCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCata                                                                                >  2:2429839/1‑93 (MQ=255)

           cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATg                                                                              <  1:115869/93‑1 (MQ=255)

           cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATg                                                                              >  1:7842097/1‑93 (MQ=255)

           cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCATGGTGATATg                                                                              >  2:4630333/1‑93 (MQ=255)

             aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCg                                                                            <  1:5519309/93‑1 (MQ=255)

              gCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCgg                                                                           >  2:6280598/1‑93 (MQ=255)

               cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGc                                                                          >  2:1258870/1‑93 (MQ=255)

                aCGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCa                                                                         >  2:1925270/1‑93 (MQ=255)

                 cgcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCaa                                                                        >  1:773037/1‑93 (MQ=255)

                    gggCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACtt                                                                     >  2:369306/1‑93 (MQ=255)

                    gggCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACtt                                                                     >  2:5118757/1‑93 (MQ=255)

                    gggCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACtt                                                                     <  1:2028328/93‑1 (MQ=255)

                     ggCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTc                                                                    <  2:6640474/93‑1 (MQ=255)

                     ggCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTc                                                                    <  1:2972947/93‑1 (MQ=255)

                      gCAGTGTCTTTAATCGACTCTTTATGACCACTCTGGCTGCCGATTGGGCCGAGATAAGTAACGCGAGAGCTCTGGGCATATGCCGAAACTTCg                                                                   >  2:1813740/1‑93 (MQ=255)

                        aGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGaa                                                                 >  2:3914423/1‑93 (MQ=255)

                         gtgtCTTTAATCGACTCTTTGTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGaaa                                                                <  1:5229033/93‑1 (MQ=255)

                         gtgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGaaa                                                                <  1:1472632/93‑1 (MQ=255)

                           gtatttAATCGACTCTTTATGACCAATCTGGCTGCAGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAaga                                                              >  2:4948009/4‑93 (MQ=255)

                           gtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAaga                                                              <  2:3768391/93‑1 (MQ=255)

                           gtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAaga                                                              >  2:4531565/1‑93 (MQ=255)

                             cTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGc                                                            <  1:6084760/93‑1 (MQ=255)

                             cTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGc                                                            >  2:4346240/1‑93 (MQ=255)

                              tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGGAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCa                                                           >  2:337503/1‑93 (MQ=255)

                               ttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAAATTCGAAAGAGCAt                                                          >  2:6538841/1‑93 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                         <  1:4299581/93‑1 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                         <  2:6052688/93‑1 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                         >  1:337241/1‑93 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                         <  2:2918745/93‑1 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                         <  1:5416122/93‑1 (MQ=255)

                                tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATc                                                         <  2:2022008/93‑1 (MQ=255)

                                   tCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg                                                      >  2:586290/1‑93 (MQ=255)

                                   tCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg                                                      <  1:2757189/93‑1 (MQ=255)

                                   tCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCggg                                                      <  1:190067/93‑1 (MQ=255)

                                       ctctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcg                                                  <  2:5317420/93‑1 (MQ=255)

                                        tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga                                                 <  2:4813685/93‑1 (MQ=255)

                                          tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt                                               >  1:3267304/1‑93 (MQ=255)

                                           ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc                                              <  2:4702195/93‑1 (MQ=255)

                                            tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcg                                             >  2:895893/1‑93 (MQ=255)

                                            tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcg                                             >  1:6888966/1‑93 (MQ=255)

                                              tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcgag                                           >  1:5069723/1‑93 (MQ=255)

                                              tGACAAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcgag                                           <  1:7004721/93‑1 (MQ=255)

                                                gcgaaTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCTCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACgagtcgagtc                                         <  1:1279347/90‑1 (MQ=255)

                                                aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACgagtcgagtc                                         <  2:1733953/93‑1 (MQ=255)

                                                 ccAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt                                        >  1:4588077/1‑93 (MQ=255)

                                                 ccAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt                                        <  1:5278833/93‑1 (MQ=255)

                                                  cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtt                                       >  2:2285407/1‑93 (MQ=255)

                                                     tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttttt                                    <  2:7260939/93‑1 (MQ=255)

                                                      cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTc                                   <  2:5247575/93‑1 (MQ=255)

                                                      cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTc                                   >  2:325423/1‑93 (MQ=255)

                                                       tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCg                                  >  2:5623988/1‑93 (MQ=255)

                                                           tGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGa                              >  1:7188733/1‑93 (MQ=255)

                                                           tGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGa                              <  2:593509/93‑1 (MQ=255)

                                                           tGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGa                              <  1:7564694/93‑1 (MQ=255)

                                                             ccGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATg                            <  2:6693049/93‑1 (MQ=255)

                                                              cGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa                           <  1:7165755/93‑1 (MQ=255)

                                                              cGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa                           <  2:7748041/93‑1 (MQ=255)

                                                              cGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa                           <  1:3569780/93‑1 (MQ=255)

                                                               gCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAg                          <  2:7453588/93‑1 (MQ=255)

                                                               gCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAg                          <  1:3882517/93‑1 (MQ=255)

                                                                cTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAgc                         >  2:7885302/1‑93 (MQ=255)

                                                                cTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAgc                         <  1:3099035/93‑1 (MQ=255)

                                                                     gCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAt                    >  1:5111915/1‑93 (MQ=255)

                                                                      ccGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATg                   <  1:4102755/93‑1 (MQ=255)

                                                                      ccGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATg                   <  1:6633986/93‑1 (MQ=255)

                                                                       cGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt                  >  2:4009851/1‑93 (MQ=255)

                                                                       cGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt                  <  2:6161529/93‑1 (MQ=255)

                                                                       cGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt                  <  1:3459191/93‑1 (MQ=255)

                                                                        gagaTAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtt                 >  2:3100410/1‑93 (MQ=255)

                                                                          gaTAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtttt               <  1:951957/93‑1 (MQ=255)

                                                                            tAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTa             <  1:4857764/93‑1 (MQ=255)

                                                                            tAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTa             <  1:6431384/93‑1 (MQ=255)

                                                                             aaGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc            >  1:6188321/1‑93 (MQ=255)

                                                                               gTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCa          <  1:1018140/93‑1 (MQ=255)

                                                                               gTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCa          >  2:2584813/1‑93 (MQ=255)

                                                                               gTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCa          <  2:6969800/93‑1 (MQ=255)

                                                                               gTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCa          <  2:6814362/93‑1 (MQ=255)

                                                                               atAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCa          <  2:2796448/92‑1 (MQ=255)

                                                                                tAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAg         <  2:1550286/93‑1 (MQ=255)

                                                                                  aCGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTg       <  1:3702020/93‑1 (MQ=255)

                                                                                  aCGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTg       <  2:1098712/93‑1 (MQ=255)

                                                                                   cgcgAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGa      >  2:6447391/1‑93 (MQ=255)

                                                                                    gcgAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAg     <  2:3476044/93‑1 (MQ=255)

                                                                                    gcgAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAg     <  1:4938692/93‑1 (MQ=255)

ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTT  >  NC_000913/4475572‑4475751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCT  >  NC_000913/4475569‑4475755

GTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCG                                                                                               >  SRR4302543.819116/1‑94 (MQ=60)

 TCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGC                                                                                              >  SRR4302543.2471915/1‑94 (MQ=60)

  CATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCC                                                                                             >  SRR4302543.6159784/1‑94 (MQ=60)

   ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCC                                                                                            >  SRR4302543.5342906/1‑94 (MQ=60)

   ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCC                                                                                            >  SRR4302543.5676106/1‑94 (MQ=60)

   ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCC                                                                                            <  SRR4302543.2155426/94‑1 (MQ=60)

   ATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCC                                                                                            >  SRR4302543.6106820/1‑94 (MQ=60)

    TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGTCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCT                                                                                           <  SRR4302543.6065049/94‑1 (MQ=60)

    TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCT                                                                                           <  SRR4302543.5293931/94‑1 (MQ=60)

    TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCT                                                                                           <  SRR4302543.3808646/94‑1 (MQ=60)

    TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCT                                                                                           <  SRR4302543.6839200/94‑1 (MQ=60)

    TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCT                                                                                           <  SRR4302543.6007816/94‑1 (MQ=60)

    TACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCT                                                                                           <  SRR4302543.6221471/94‑1 (MQ=60)

     ACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTG                                                                                          <  SRR4302543.46387/94‑1 (MQ=60)

     ACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTG                                                                                          <  SRR4302543.4439590/94‑1 (MQ=60)

     ACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTG                                                                                          <  SRR4302543.340322/94‑1 (MQ=60)

     ACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTGATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTG                                                                                          <  SRR4302543.2054280/94‑1 (MQ=60)

      CATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGG                                                                                         >  SRR4302543.1832357/1‑94 (MQ=60)

      CATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGG                                                                                         >  SRR4302543.3655581/1‑94 (MQ=60)

      CATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGG                                                                                         <  SRR4302543.1228254/94‑1 (MQ=60)

      CATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGG                                                                                         >  SRR4302543.1811865/1‑94 (MQ=60)

       ATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGT                                                                                        >  SRR4302543.5856986/1‑94 (MQ=60)

       ATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGT                                                                                        <  SRR4302543.7479707/94‑1 (MQ=60)

        AGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTC                                                                                       <  SRR4302543.6644707/94‑1 (MQ=60)

         GCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCA                                                                                      >  SRR4302543.5253000/1‑94 (MQ=60)

         GCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCA                                                                                      <  SRR4302543.2563249/94‑1 (MQ=60)

          CGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAT                                                                                     <  SRR4302543.5024183/94‑1 (MQ=60)

          CGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAT                                                                                     >  SRR4302543.6466784/1‑94 (MQ=60)

          CGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAT                                                                                     >  SRR4302543.5251324/1‑94 (MQ=60)

           GACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCTAGCTAAGTAACGCGAGCGCCCTCGACATA                                                                                    >  SRR4302543.387271/1‑94 (MQ=60)

            ACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATAT                                                                                   >  SRR4302543.761094/1‑94 (MQ=60)

            ACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATAT                                                                                   <  SRR4302543.3098284/94‑1 (MQ=60)

             CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATG                                                                                  <  SRR4302543.6717494/94‑1 (MQ=60)

             CCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATG                                                                                  >  SRR4302543.7916020/1‑94 (MQ=60)

              CAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGC                                                                                 <  SRR4302543.116920/94‑1 (MQ=60)

              CAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGC                                                                                 >  SRR4302543.2263240/1‑94 (MQ=60)

              CAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGC                                                                                 <  SRR4302543.5512547/94‑1 (MQ=60)

              CAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGC                                                                                 <  SRR4302543.116921/94‑1 (MQ=60)

                AGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGG                                                                               >  SRR4302543.7812383/1‑94 (MQ=60)

                AGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGG                                                                               <  SRR4302543.3533713/94‑1 (MQ=60)

                AGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGG                                                                               <  SRR4302543.5569440/94‑1 (MQ=60)

                AGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGG                                                                               >  SRR4302543.6867592/1‑94 (MQ=60)

                AGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGG                                                                               >  SRR4302543.6867590/1‑94 (MQ=60)

                   ACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAC                                                                            >  SRR4302543.430044/1‑94 (MQ=60)

                   ACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAA                                                                            >  SRR4302543.779820/1‑94 (MQ=60)

                    CGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAAC                                                                           <  SRR4302543.2351131/94‑1 (MQ=60)

                    CGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAAC                                                                           <  SRR4302543.6016200/94‑1 (MQ=60)

                    CGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAAC                                                                           <  SRR4302543.1915673/94‑1 (MQ=60)

                    CGCGGGAAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAAC                                                                           <  SRR4302543.5515471/94‑1 (MQ=60)

                     GCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACT                                                                          <  SRR4302543.2047498/94‑1 (MQ=60)

                       GGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTC                                                                        <  SRR4302543.2046558/94‑1 (MQ=60)

                       GGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTC                                                                        >  SRR4302543.840275/1‑94 (MQ=60)

                        GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCG                                                                       <  SRR4302543.2999941/94‑1 (MQ=60)

                        GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCG                                                                       >  SRR4302543.440348/1‑94 (MQ=60)

                        GGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCG                                                                       <  SRR4302543.3037234/94‑1 (MQ=60)

                         GCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGA                                                                      >  SRR4302543.5073607/1‑94 (MQ=60)

                           AGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAA                                                                    >  SRR4302543.3972875/1‑94 (MQ=60)

                           AGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAA                                                                    >  SRR4302543.3972874/1‑94 (MQ=60)

                           AGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAA                                                                    >  SRR4302543.7139379/1‑94 (MQ=60)

                            GTGTCTTTAATCGACTCTTTGTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAG                                                                   <  SRR4302543.5276143/94‑1 (MQ=60)

                            GTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAG                                                                   <  SRR4302543.1485897/94‑1 (MQ=60)

                            GTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAG                                                                   >  SRR4302543.1008783/1‑94 (MQ=60)

                             TGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGA                                                                  <  SRR4302543.4353167/94‑1 (MQ=60)

                              GTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAG                                                                 <  SRR4302543.319934/94‑1 (MQ=60)

                               TCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGC                                                                <  SRR4302543.6062534/94‑1 (MQ=60)

                                TTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               <  SRR4302543.5760083/94‑1 (MQ=60)

                                CTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               <  SRR4302543.2850543/94‑1 (MQ=60)

                                CTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               <  SRR4302543.6142425/94‑1 (MQ=60)

                                CTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               >  SRR4302543.1916401/1‑94 (MQ=60)

                                CTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               <  SRR4302543.742733/94‑1 (MQ=60)

                                CTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCA                                                               <  SRR4302543.4232918/94‑1 (MQ=60)

                                 TTTAATCGACTCTTTATGACCAATCTGTCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCAT                                                              <  SRR4302543.7799146/94‑1 (MQ=60)

                                 TTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCAT                                                              <  SRR4302543.4501764/94‑1 (MQ=60)

                                  TTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATC                                                             >  SRR4302543.340337/1‑94 (MQ=60)

                                   TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCG                                                            <  SRR4302543.6620770/94‑1 (MQ=60)

                                   TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCG                                                            <  SRR4302543.5465393/94‑1 (MQ=60)

                                   TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCG                                                            <  SRR4302543.4338649/94‑1 (MQ=60)

                                   TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCG                                                            <  SRR4302543.6778624/94‑1 (MQ=60)

                                    AATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGG                                                           >  SRR4302543.341230/1‑94 (MQ=60)

                                     ATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGG                                                          >  SRR4302543.2581394/1‑94 (MQ=60)

                                     ATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGG                                                          <  SRR4302543.2719791/94‑1 (MQ=60)

                                     ATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGG                                                          >  SRR4302543.1415754/1‑94 (MQ=60)

                                      TCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGT                                                         <  SRR4302543.191677/94‑1 (MQ=60)

                                      TCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGT                                                         <  SRR4302543.2782269/94‑1 (MQ=60)

                                      TCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGT                                                         <  SRR4302543.3146044/94‑1 (MQ=60)

                                       CGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTA                                                        >  SRR4302543.5768321/1‑94 (MQ=60)

                                       CGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTA                                                        >  SRR4302543.3658423/1‑94 (MQ=60)

                                        GACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAC                                                       >  SRR4302543.5633688/1‑94 (MQ=60)

                                         CCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACG                                                      <  SRR4302543.917193/94‑1 (MQ=60)

                                         ACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACG                                                      <  SRR4302543.2339444/94‑1 (MQ=60)

                                         ACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACG                                                      >  SRR4302543.3865737/1‑94 (MQ=60)

                                          CTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAGCTTCGAAAGAGCATCGGGTACGA                                                     >  SRR4302543.7151662/1‑94 (MQ=60)

                                          CTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGG                                                     >  SRR4302543.5633399/1‑94 (MQ=60)

                                          CTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACCA                                                     >  SRR4302543.5119537/1‑94 (MQ=60)

                                            gtttatgcccaaccgtgctgccttgTGGGCGGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGT                                                   <  SRR4302543.3898836/69‑1 (MQ=48)

                                            CTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGT                                                   >  SRR4302543.3296828/1‑94 (MQ=60)

                                             TTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTC                                                  <  SRR4302543.5401982/94‑1 (MQ=60)

                                             TTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTC                                                  <  SRR4302543.457762/94‑1 (MQ=60)

                                              TTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCG                                                 >  SRR4302543.6953752/1‑94 (MQ=60)

                                               TATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGA                                                <  SRR4302543.4908582/94‑1 (MQ=60)

                                               TATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGA                                                <  SRR4302543.7249221/94‑1 (MQ=60)

                                               TATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGA                                                >  SRR4302543.7255444/1‑94 (MQ=60)

                                               TATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGACATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGA                                                >  SRR4302543.2080847/1‑94 (MQ=60)

                                                ATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAG                                               >  SRR4302543.1637111/1‑94 (MQ=60)

                                                ATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAG                                               >  SRR4302543.5115740/1‑94 (MQ=60)

                                                 TGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGT                                              <  SRR4302543.4635806/94‑1 (MQ=60)

                                                 TGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGT                                              <  SRR4302543.7347542/94‑1 (MQ=60)

                                                 TGACAAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGT                                              <  SRR4302543.7071265/94‑1 (MQ=60)

                                                  GACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTC                                             >  SRR4302543.3417077/1‑94 (MQ=60)

                                                   gcgAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCTCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT                                            <  SRR4302543.1290755/91‑1 (MQ=60)

                                                   ACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT                                            >  SRR4302543.4629754/1‑94 (MQ=60)

                                                   ACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT                                            >  SRR4302543.1379150/1‑94 (MQ=60)

                                                   ACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCAGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT                                            <  SRR4302543.3450503/94‑1 (MQ=60)

                                                    CCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTT                                           <  SRR4302543.3002264/94‑1 (MQ=60)

                                                    CCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTT                                           <  SRR4302543.5326468/94‑1 (MQ=60)

                                                     CAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTT                                          >  SRR4302543.3494006/1‑94 (MQ=60)

                                                     CAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTT                                          >  SRR4302543.7805221/1‑94 (MQ=60)

                                                       ATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTT                                        <  SRR4302543.121242/94‑1 (MQ=60)

                                                       ATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTT                                        >  SRR4302543.3728671/1‑94 (MQ=60)

                                                       ATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTT                                        <  SRR4302543.7183543/94‑1 (MQ=60)

                                                        TCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTC                                       <  SRR4302543.3540051/94‑1 (MQ=60)

                                                        TCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCAGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTC                                       <  SRR4302543.5283716/94‑1 (MQ=60)

                                                         CTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCG                                      <  SRR4302543.335747/94‑1 (MQ=60)

                                                         CTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCG                                      >  SRR4302543.5542995/1‑94 (MQ=60)

                                                         CTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCG                                      >  SRR4302543.6262827/1‑94 (MQ=60)

                                                         CTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCG                                      >  SRR4302543.808547/1‑94 (MQ=60)

                                                         CTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCG                                      >  SRR4302543.2948165/1‑94 (MQ=60)

                                                         CTGGCTGCCGCTTGGGCAGACATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCG                                      <  SRR4302543.7034447/94‑1 (MQ=58)

                                                          TGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGA                                     >  SRR4302543.4944461/1‑94 (MQ=60)

                                                           GGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAA                                    <  SRR4302543.6940380/94‑1 (MQ=60)

                                                             CTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGA                                  >  SRR4302543.1192763/1‑94 (MQ=60)

                                                             CTGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGA                                  >  SRR4302543.7258464/1‑94 (MQ=60)

                                                              TGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAT                                 <  SRR4302543.7386794/94‑1 (MQ=60)

                                                              TGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAT                                 <  SRR4302543.7636686/94‑1 (MQ=60)

                                                              TGCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAT                                 <  SRR4302543.3593574/94‑1 (MQ=60)

                                                              TGCCGCTTGGGCAGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAT                                 <  SRR4302543.4386998/94‑1 (MQ=60)

                                                               GCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATG                                <  SRR4302543.5127200/94‑1 (MQ=60)

                                                               GCCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATG                                <  SRR4302543.5588235/94‑1 (MQ=60)

                                                                CCGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGA                               >  SRR4302543.2873823/1‑94 (MQ=60)

                                                                 CGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAG                              <  SRR4302543.3601343/94‑1 (MQ=60)

                                                                 CGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAG                              <  SRR4302543.7235356/94‑1 (MQ=60)

                                                                 CGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAG                              <  SRR4302543.1900737/94‑1 (MQ=60)

                                                                 CGCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAG                              >  SRR4302543.5303200/1‑94 (MQ=60)

                                                                  cccTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCAGATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGC                             <  SRR4302543.1928527/91‑1 (MQ=44)

                                                                  GCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGC                             >  SRR4302543.7516267/1‑94 (MQ=60)

                                                                  GCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGC                             <  SRR4302543.3916796/94‑1 (MQ=60)

                                                                  GCTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGC                             <  SRR4302543.1661986/94‑1 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            <  SRR4302543.6100435/94‑1 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            >  SRR4302543.4935508/1‑94 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            >  SRR4302543.5061378/1‑94 (MQ=60)

                                                                   CTTGGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCG                            <  SRR4302543.3126910/94‑1 (MQ=60)

                                                                      GGGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGAACGAGTCGAGTCTTTTTCGAAGATGAGCGCGA                         >  SRR4302543.1375849/1‑94 (MQ=53)

                                                                       GGCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAT                        >  SRR4302543.5158166/1‑94 (MQ=53)

                                                                       GGCAGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAT                        <  SRR4302543.625293/94‑1 (MQ=41)

                                                                        GCCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATG                       <  SRR4302543.7660398/94‑1 (MQ=50)

                                                                         CCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGT                      <  SRR4302543.4139650/94‑1 (MQ=48)

                                                                         CCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGT                      <  SRR4302543.6696579/94‑1 (MQ=48)

                                                                         CCGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGT                      >  SRR4302543.4551713/1‑94 (MQ=48)

                                                                          CGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTT                     >  SRR4302543.33119/1‑94 (MQ=45)

                                                                          CGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTT                     >  SRR4302543.6956765/1‑94 (MQ=45)

                                                                          CGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTT                     >  SRR4302543.5408866/1‑94 (MQ=45)

                                                                          CGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTT                     >  SRR4302543.7687263/1‑94 (MQ=45)

                                                                          CGAGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTT                     <  SRR4302543.3490182/94‑1 (MQ=45)

                                                                            AGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTT                   >  SRR4302543.6966168/1‑94 (MQ=40)

                                                                            AGATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTT                   >  SRR4302543.4639390/1‑94 (MQ=40)

                                                                             GATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTT                  <  SRR4302543.960275/94‑1 (MQ=38)

                                                                              ATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTA                 <  SRR4302543.1125144/94‑1 (MQ=35)

                                                                              ATAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTA                 >  SRR4302543.7634184/1‑94 (MQ=35)

                                                                               TAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC                <  SRR4302543.6763284/94‑1 (MQ=33)

                                                                               TAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC                <  SRR4302543.6491982/94‑1 (MQ=33)

                                                                               TAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC                >  SRR4302543.6246788/1‑94 (MQ=33)

                                                                               TAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC                <  SRR4302543.5160119/94‑1 (MQ=33)

                                                                               TAAGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC                <  SRR4302543.4902057/94‑1 (MQ=33)

                                                                                 AGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCA              >  SRR4302543.3792264/1‑94 (MQ=30)

                                                                                 AGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGACTTTTTCGAAGATGAGCGCGATGTTTTTACCA              >  SRR4302543.6156313/1‑94 (MQ=29)

                                                                                 AGTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGAACGAGTCGAGTAGTTTGCGAAGATGAGGGCGATGTctttagcg              >  SRR4302543.3792267/1‑86 (MQ=22)

                                                                                  GTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAG             <  SRR4302543.1027146/94‑1 (MQ=30)

                                                                                  GTAACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAG             <  SRR4302543.2035264/94‑1 (MQ=30)

                                                                                     ACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGA          <  SRR4302543.3734564/94‑1 (MQ=38)

                                                                                     ACGCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGA          <  SRR4302543.7864480/94‑1 (MQ=38)

                                                                                       GCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGT        <  SRR4302543.4983666/94‑1 (MQ=40)

                                                                                       GCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGT        <  SRR4302543.4262790/94‑1 (MQ=40)

                                                                                       GCGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGT        <  SRR4302543.3143/94‑1 (MQ=40)

                                                                                        CGAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTT       >  SRR4302543.4016476/1‑94 (MQ=40)

                                                                                         GAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTT      <  SRR4302543.2009696/94‑1 (MQ=40)

                                                                                         GAGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTT      <  SRR4302543.6601020/94‑1 (MQ=40)

                                                                                          AGCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTG     <  SRR4302543.5805881/94‑1 (MQ=43)

                                                                                           GCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGG    <  SRR4302543.2846783/94‑1 (MQ=46)

                                                                                           GCGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTAGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGG    <  SRR4302543.7660536/94‑1 (MQ=44)

                                                                                            CGCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGC   >  SRR4302543.5891628/1‑94 (MQ=52)

                                                                                             GCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCT  <  SRR4302543.5464692/94‑1 (MQ=57)

                                                                                             GCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCT  >  SRR4302543.2201728/1‑94 (MQ=57)

                                                                                             GCCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCT  <  SRR4302543.7047703/94‑1 (MQ=57)

GTCATACATGCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCT  >  NC_000913/4475569‑4475755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`