Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	647,331	G→A	D488D (GAC→GAT)	citF ←	citrate lyase alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	647,331	0	G	A	99.7%	1028.8 / ‑5.7	285	D488D (GAC→GAT)	citF	citrate lyase alpha chain
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (166/118); total (167/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 298 total aligned reads displayed.

TTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTC  >  NC_000913/647241‑647420

ttCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATc                                                                                         >  1:8415650/1‑93 (MQ=255)

ttCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATc                                                                                         >  1:7078079/1‑93 (MQ=255)

 tCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCt                                                                                        >  2:9505373/1‑93 (MQ=255)

  cAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCtg                                                                                       <  1:7767018/93‑1 (MQ=255)

  cAGGAAGCAGCTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCtg                                                                                       <  1:5626264/93‑1 (MQ=255)

   aGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCtgt                                                                                      >  2:5994528/1‑93 (MQ=255)

    ggAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCtgtg                                                                                     >  1:1407994/1‑93 (MQ=255)

     gAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGa                                                                                    >  1:6441680/1‑93 (MQ=255)

     gAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGa                                                                                    >  1:4565453/1‑93 (MQ=255)

     gAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGa                                                                                    >  2:2396699/1‑93 (MQ=255)

           ggTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCaa                                                                              >  2:3165386/1‑93 (MQ=255)

           ggTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCaa                                                                              >  1:6781406/1‑93 (MQ=255)

           ggTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGGAACGCACAACGGCAACGACGCGATCTGTGAATTCaa                                                                              >  2:3457002/1‑93 (MQ=255)

            gTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAt                                                                             >  2:9683647/1‑93 (MQ=255)

             tGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATc                                                                            <  1:5290682/93‑1 (MQ=255)

             tGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATc                                                                            <  2:4364347/93‑1 (MQ=255)

              gCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCg                                                                           >  1:8101024/1‑93 (MQ=255)

                aTGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGc                                                                         >  1:3542322/1‑93 (MQ=255)

                 tGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCt                                                                        >  1:5686732/1‑93 (MQ=255)

                 tGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCt                                                                        >  1:441249/1‑93 (MQ=255)

                   gCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCtgt                                                                      <  2:1276040/93‑1 (MQ=255)

                    cTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCtgtg                                                                     <  2:8742052/93‑1 (MQ=255)

                    cTTATTCCTCCCCCTGATGCACAACATCGATCGCCGAGGCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCtgtg                                                                     <  1:3087436/93‑1 (MQ=255)

                     ttATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTgg                                                                    >  1:3854726/1‑93 (MQ=255)

                      tATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGt                                                                   >  2:2542220/1‑93 (MQ=255)

                       aTTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGtt                                                                  <  2:452989/93‑1 (MQ=255)

                       aTTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGtt                                                                  >  2:6572781/1‑93 (MQ=255)

                       aTTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGtt                                                                  <  1:1230939/93‑1 (MQ=255)

                        ttCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGCTc                                                                 >  1:6049603/1‑93 (MQ=255)

                            ttCACCTGATGCACAACATCGATCACCGAGCCATCTCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCg                                                             >  1:232018/1‑93 (MQ=255)

                            ttCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCg                                                             >  1:2515152/1‑93 (MQ=255)

                            tacACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCg                                                             <  2:5686731/91‑1 (MQ=255)

                            ctCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACACCGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCg                                                             <  1:2632470/92‑1 (MQ=255)

                             tCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCgg                                                            <  1:6724235/93‑1 (MQ=255)

                              cACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGt                                                           >  2:8896538/1‑93 (MQ=255)

                              cACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGt                                                           <  2:9795333/93‑1 (MQ=255)

                               cccTGATGCACAACTTCGGTCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTc                                                          <  1:8924031/92‑1 (MQ=255)

                               aCCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGCATTCAATCGGCTGTGGTTCACCGGTc                                                          >  2:9911225/1‑93 (MQ=255)

                                ccTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTca                                                         <  1:4629439/93‑1 (MQ=255)

                                 cTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTcag                                                        >  1:4166634/1‑93 (MQ=255)

                                  tGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTcagc                                                       >  2:3434170/1‑93 (MQ=255)

                                   ggtGCACAACAGTGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTcagca                                                      <  1:8560265/91‑1 (MQ=255)

                                   gATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTcagca                                                      <  2:1803643/93‑1 (MQ=255)

                                    aTGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTcagcag                                                     <  2:9360385/93‑1 (MQ=255)

                                     tGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGa                                                    >  1:9883083/1‑93 (MQ=255)

                                     agCACAAAATCGAGCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTTAATTCAATCGGCTGTGGTTCACCGGTCAGCAGa                                                    <  1:8316763/92‑1 (MQ=255)

                                        acaacaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGAcgc                                                 >  2:626291/1‑93 (MQ=255)

                                         caacaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGACGGAGGGTAACCGCGCAACAGAcgcc                                                >  1:360700/1‑92 (MQ=255)

                                           acaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGTTCAGCAGACgcgcc                                              >  2:7251386/1‑92 (MQ=255)

                                           acaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCa                                              <  2:8549500/93‑1 (MQ=255)

                                             aTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACg                                            >  1:1696791/1‑93 (MQ=255)

                                             aTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACg                                            >  2:9089630/1‑93 (MQ=255)

                                              tCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTGAGCAGACGCGCACGt                                           >  1:5077106/1‑93 (MQ=255)

                                              tCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGt                                           >  1:4775162/1‑93 (MQ=255)

                                              tCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGt                                           >  1:5772483/1‑93 (MQ=255)

                                               cGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGtt                                          <  2:7164267/93‑1 (MQ=255)

                                                gATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTc                                         <  2:4670137/93‑1 (MQ=255)

                                                 aTCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTcg                                        >  1:8278768/1‑93 (MQ=255)

                                                 aTCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTcg                                        >  1:5476471/1‑93 (MQ=255)

                                                 aTCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGGTCACCGGTCAGCAGACGCGCACGTTcg                                        >  1:4028051/1‑93 (MQ=255)

                                                  tCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGAGCACGTTcgc                                       >  1:2749069/1‑93 (MQ=255)

                                                   cACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTcgcg                                      >  2:8019934/1‑93 (MQ=255)

                                                     ccGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCa                                    >  2:1487137/1‑93 (MQ=255)

                                                     ccGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCa                                    <  2:8368455/93‑1 (MQ=255)

                                                      cGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAg                                   <  1:1511602/93‑1 (MQ=255)

                                                      cGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAg                                   <  1:1919007/93‑1 (MQ=255)

                                                       gAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGc                                  >  1:8740393/1‑93 (MQ=255)

                                                          ccATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGcccc                               >  2:3094827/1‑91 (MQ=255)

                                                          ccATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCAc                               >  2:1085298/1‑93 (MQ=255)

                                                           cATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACt                              <  2:1981686/93‑1 (MQ=255)

                                                            aTCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTc                             >  2:6781220/1‑93 (MQ=255)

                                                            aTCGCGGGAAAGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTc                             >  2:8518755/1‑93 (MQ=255)

                                                             tCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCa                            >  1:2673735/1‑93 (MQ=255)

                                                             tCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCa                            >  2:6043012/1‑93 (MQ=255)

                                                               gcgGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAAt                          <  2:5587157/93‑1 (MQ=255)

                                                               gcgGTAACGAACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGGTCGCGCAGCCACTCAAt                          >  2:7511518/1‑93 (MQ=255)

                                                                 ggTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATgg                        <  2:8834568/93‑1 (MQ=255)

                                                                  gTAACGCACACCGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGa                       <  1:7918464/93‑1 (MQ=255)

                                                                  gTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGa                       >  1:130163/1‑93 (MQ=255)

                                                                  gTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGa                       <  1:6173821/93‑1 (MQ=255)

                                                                  gTAACGCACAACGGCAACGACGCGAACTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCaaccga                       >  2:421859/1‑89 (MQ=255)

                                                                     aCGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAAc                    >  1:2443710/1‑93 (MQ=255)

                                                                      cGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTTGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAAcc                   <  1:9500826/93‑1 (MQ=255)

                                                                      cGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAAcc                   <  1:1123722/93‑1 (MQ=255)

                                                                          caACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCTGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCAcct               >  1:1417194/1‑91 (MQ=255)

                                                                          caACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTTGCGCAGCCACTCAATGGAAACCACtt               >  2:9945358/1‑93 (MQ=255)

                                                                          caACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACtt               >  2:3992616/1‑93 (MQ=255)

                                                                             cGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTaa            >  1:8217862/1‑93 (MQ=255)

                                                                             cGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTaa            <  1:5994529/93‑1 (MQ=255)

                                                                             cGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTaa            <  1:4248608/93‑1 (MQ=255)

                                                                             cGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTaa            <  1:1014638/93‑1 (MQ=255)

                                                                              gtcAACGACGCGAGCTGTGAATTCAATCGGCGGTGGTTCACCGGTCAGCAGACGCGCACGGTCGCGCAGCCACTCAATGGAAACCACTTTAAt           <  1:6862486/91‑1 (MQ=255)

                                                                              ggCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAt           <  2:2819032/93‑1 (MQ=255)

                                                                               gCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGCTCGCGCAGCCACTCAATGGAAACCACTTTAATg          >  2:6132293/1‑93 (MQ=255)

                                                                                 aaCGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGcc        <  1:7411344/93‑1 (MQ=255)

                                                                                  acgacgCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGccc       <  2:698104/93‑1 (MQ=255)

                                                                                  acgacgCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGccc       >  2:4901546/1‑93 (MQ=255)

                                                                                  acgacgCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGccc       >  1:3755506/1‑93 (MQ=255)

                                                                                     acgCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCt    >  1:6960724/1‑93 (MQ=255)

                                                                                     acgCGATCTGTGAATTCAACCGCCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCt    <  2:7616923/93‑1 (MQ=255)

TTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTC  >  NC_000913/647241‑647420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTGC  >  NC_000913/647239‑647424

AGTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGAT                                                                                              >  SRR4302540.6140502/1‑94 (MQ=60)

AGTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGAT                                                                                              >  SRR4302540.2831608/1‑94 (MQ=60)

AGTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATCCACAACATCTATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGAT                                                                                              <  SRR4302540.2556368/94‑1 (MQ=60)

 GTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATC                                                                                             >  SRR4302540.7156846/1‑94 (MQ=60)

 GTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATC                                                                                             >  SRR4302540.8513328/1‑94 (MQ=60)

 GTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGGAACGCACAACGGCAAAGACGCGATC                                                                                             >  SRR4302540.4206101/1‑94 (MQ=60)

   TCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTG                                                                                           >  SRR4302540.8622325/1‑94 (MQ=60)

    CAGGAAGCAGGTGCATGGCTTATTCCTTCGCCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGT                                                                                          <  SRR4302540.7857825/94‑1 (MQ=60)

    CAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGT                                                                                          <  SRR4302540.5688196/94‑1 (MQ=60)

    CAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGT                                                                                          <  SRR4302540.7857821/94‑1 (MQ=60)

    CAGGAAGCAGCTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGT                                                                                          <  SRR4302540.5688194/94‑1 (MQ=60)

     AGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTG                                                                                         >  SRR4302540.4513744/1‑94 (MQ=60)

     AGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTG                                                                                         >  SRR4302540.1423153/1‑94 (MQ=60)

     AGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATAACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTG                                                                                         >  SRR4302540.423511/1‑94 (MQ=60)

      GGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGA                                                                                        >  SRR4302540.4614752/1‑94 (MQ=60)

      GGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGA                                                                                        >  SRR4302540.2549288/1‑94 (MQ=60)

      GGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGA                                                                                        >  SRR4302540.6513375/1‑94 (MQ=60)

       GAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAA                                                                                       >  SRR4302540.658843/1‑94 (MQ=60)

          GCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTC                                                                                    >  SRR4302540.6895865/1‑94 (MQ=60)

           CAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCA                                                                                   <  SRR4302540.3729496/94‑1 (MQ=60)

           CAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCA                                                                                   >  SRR4302540.1981533/1‑94 (MQ=60)

           CAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCA                                                                                   >  SRR4302540.1749456/1‑94 (MQ=60)

           CAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCA                                                                                   <  SRR4302540.3866959/94‑1 (MQ=60)

           CAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCA                                                                                   <  SRR4302540.3729497/94‑1 (MQ=60)

           CAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCA                                                                                   >  SRR4302540.9386588/1‑94 (MQ=60)

            AGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAA                                                                                  >  SRR4302540.6856390/1‑94 (MQ=60)

            AGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAA                                                                                  <  SRR4302540.3493310/94‑1 (MQ=60)

             GGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAT                                                                                 >  SRR4302540.2124826/1‑94 (MQ=60)

             GGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAT                                                                                 <  SRR4302540.7064221/94‑1 (MQ=60)

              GTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATC                                                                                >  SRR4302540.3835981/1‑94 (MQ=60)

              GTGCAGGGCTTATTCCTTCGCCTAATGTACAACATCGATCGCCGAGCCGTCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATC                                                                                <  SRR4302540.4523191/94‑1 (MQ=60)

               TGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCG                                                                               >  SRR4302540.8195048/1‑94 (MQ=60)

               TGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCG                                                                               <  SRR4302540.2394408/94‑1 (MQ=60)

               TGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCG                                                                               >  SRR4302540.6178040/1‑94 (MQ=60)

               TGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCG                                                                               <  SRR4302540.5709622/94‑1 (MQ=60)

               TGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCG                                                                               <  SRR4302540.5348519/94‑1 (MQ=60)

                 CATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGC                                                                             >  SRR4302540.3581014/1‑94 (MQ=60)

                 CATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGC                                                                             >  SRR4302540.5322169/1‑94 (MQ=60)

                 CATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGC                                                                             <  SRR4302540.1274849/94‑1 (MQ=60)

                  ATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCT                                                                            >  SRR4302540.445945/1‑94 (MQ=60)

                  ATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCT                                                                            >  SRR4302540.5749330/1‑94 (MQ=60)

                  ATGGCTTATTCCCTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCT                                                                            >  SRR4302540.5847076/1‑94 (MQ=60)

                   TGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTG                                                                           >  SRR4302540.2411350/1‑94 (MQ=60)

                   TGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTG                                                                           >  SRR4302540.5768369/1‑94 (MQ=60)

                   TGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTG                                                                           >  SRR4302540.5768368/1‑94 (MQ=60)

                   TGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTG                                                                           <  SRR4302540.6502867/94‑1 (MQ=60)

                    GGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGT                                                                          >  SRR4302540.4295292/1‑94 (MQ=60)

                    GGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGT                                                                          >  SRR4302540.1292725/1‑94 (MQ=60)

                     GCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTG                                                                         >  SRR4302540.5646089/1‑94 (MQ=60)

                      CTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGG                                                                        >  SRR4302540.3896635/1‑94 (MQ=60)

                      CTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGG                                                                        >  SRR4302540.5981559/1‑94 (MQ=60)

                      CTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGG                                                                        <  SRR4302540.3121310/94‑1 (MQ=60)

                      CTTATTCCTCCCCCTGATGCACAACATCGATCGCCGAGGCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGG                                                                        <  SRR4302540.3121311/94‑1 (MQ=60)

                       TTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGT                                                                       >  SRR4302540.2771277/1‑94 (MQ=60)

                         ATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTC                                                                     >  SRR4302540.9920987/1‑94 (MQ=60)

                         ATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTC                                                                     <  SRR4302540.1243514/94‑1 (MQ=60)

                         ATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGCTC                                                                     >  SRR4302540.6116834/1‑94 (MQ=60)

                          TTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACACCGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCA                                                                    >  SRR4302540.7015410/1‑94 (MQ=60)

                          TTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCA                                                                    >  SRR4302540.6116250/1‑94 (MQ=60)

                           TCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCAC                                                                   >  SRR4302540.1209311/1‑94 (MQ=60)

                           TCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCAC                                                                   >  SRR4302540.1324707/1‑94 (MQ=60)

                           TCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCAC                                                                   <  SRR4302540.8258963/94‑1 (MQ=60)

                             CTTCACCTGATGCACAACATCGATCACCGAGCCATCTCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCG                                                                 >  SRR4302540.234258/1‑94 (MQ=60)

                             CTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCG                                                                 >  SRR4302540.2542326/1‑94 (MQ=60)

                             CTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCG                                                                 >  SRR4302540.1427461/1‑94 (MQ=60)

                             CTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCG                                                                 >  SRR4302540.8353116/1‑94 (MQ=60)

                             CTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCG                                                                 >  SRR4302540.1583485/1‑94 (MQ=60)

                              CTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACACCGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGG                                                                <  SRR4302540.2660964/94‑1 (MQ=60)

                              CTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGG                                                                <  SRR4302540.2660965/94‑1 (MQ=60)

                               TCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGT                                                               <  SRR4302540.6798459/94‑1 (MQ=60)

                               GCACCTGATGCACAACATCGATCCCCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGT                                                               <  SRR4302540.6798460/94‑1 (MQ=60)

                                 CCCTGATGCACAACTTCGGTCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCA                                                             <  SRR4302540.9027682/94‑1 (MQ=60)

                                 ACCTGAGGCCCAACATCGATCACCGAGCCATCGCGGTAACTCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCA                                                             <  SRR4302540.7335146/94‑1 (MQ=60)

                                  CCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAG                                                            >  SRR4302540.4211809/1‑94 (MQ=60)

                                  CCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAG                                                            <  SRR4302540.4679028/94‑1 (MQ=60)

                                   CTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGC                                                           <  SRR4302540.8164340/94‑1 (MQ=60)

                                   CTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGC                                                           <  SRR4302540.1031291/94‑1 (MQ=60)

                                     GGTGCACAACAGTGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAG                                                         <  SRR4302540.8659687/94‑1 (MQ=60)

                                     GATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAG                                                         <  SRR4302540.8659688/94‑1 (MQ=60)

                                      ATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGA                                                        >  SRR4302540.9998485/1‑94 (MQ=60)

                                       AGCACAAAATCGAGCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTTAATTCAATCGGCTGTGGTTCACCGGTCAGCAGAC                                                       <  SRR4302540.8413308/94‑1 (MQ=60)

                                          ACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGacggagggtaaccgcgcaacagacgcc                                                    >  SRR4302540.364406/1‑67 (MQ=60)

                                          ACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCG                                                    >  SRR4302540.4811994/1‑94 (MQ=60)

                                            AACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCA                                                  >  SRR4302540.5104818/1‑94 (MQ=60)

                                            AACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCA                                                  >  SRR4302540.6371500/1‑94 (MQ=60)

                                             ACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCAC                                                 <  SRR4302540.9620353/94‑1 (MQ=60)

                                             ACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCAC                                                 <  SRR4302540.8215351/94‑1 (MQ=60)

                                             ACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCAC                                                 <  SRR4302540.2669977/94‑1 (MQ=60)

                                              CATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACG                                                >  SRR4302540.1715174/1‑94 (MQ=60)

                                               ATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTGAGCAGACGCGCACGT                                               >  SRR4302540.5132001/1‑94 (MQ=60)

                                               ATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGT                                               >  SRR4302540.4826175/1‑94 (MQ=60)

                                               ATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGT                                               <  SRR4302540.6667824/94‑1 (MQ=60)

                                               ATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGT                                               <  SRR4302540.4019765/94‑1 (MQ=60)

                                               ATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGT                                               >  SRR4302540.5836248/1‑94 (MQ=60)

                                                TCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTT                                              >  SRR4302540.3628209/1‑94 (MQ=60)

                                                 CGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTC                                             <  SRR4302540.4849244/94‑1 (MQ=60)

                                                  GATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCG                                            >  SRR4302540.8374927/1‑94 (MQ=60)

                                                  GATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCG                                            >  SRR4302540.5536749/1‑94 (MQ=60)

                                                  GATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCG                                            >  SRR4302540.5536750/1‑94 (MQ=60)

                                                  GATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGGTCACCGGTCAGCAGACGCGCACGTTCG                                            >  SRR4302540.4071487/1‑94 (MQ=60)

                                                   ATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGC                                           >  SRR4302540.283982/1‑94 (MQ=60)

                                                   ATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGC                                           >  SRR4302540.1860171/1‑94 (MQ=60)

                                                   ATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGAGCACGTTCGC                                           >  SRR4302540.2778770/1‑94 (MQ=60)

                                                     CACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGC                                         >  SRR4302540.1205499/1‑94 (MQ=60)

                                                        CGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGC                                      <  SRR4302540.1939943/94‑1 (MQ=60)

                                                        CGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGC                                      <  SRR4302540.1528302/94‑1 (MQ=60)

                                                        CGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGC                                      >  SRR4302540.8841855/1‑94 (MQ=60)

                                                          AGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCA                                    >  SRR4302540.9193986/1‑94 (MQ=60)

                                                          AGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCA                                    <  SRR4302540.8480073/94‑1 (MQ=60)

                                                          AGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCA                                    <  SRR4302540.143496/94‑1 (MQ=60)

                                                          AGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCA                                    <  SRR4302540.7575605/94‑1 (MQ=60)

                                                            CCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACT                                  >  SRR4302540.7487567/1‑94 (MQ=60)

                                                             CATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTC                                 >  SRR4302540.2579372/1‑94 (MQ=60)

                                                              ATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCA                                >  SRR4302540.711236/1‑94 (MQ=60)

                                                              ATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCA                                >  SRR4302540.2702683/1‑94 (MQ=60)

                                                                CGCGGTAACGCACAGCGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAAT                              <  SRR4302540.10092270/94‑1 (MQ=60)

                                                                CGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAAT                              <  SRR4302540.5653802/94‑1 (MQ=60)

                                                                  CGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGG                            >  SRR4302540.7732895/1‑94 (MQ=60)

                                                                   GGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGA                           >  SRR4302540.131532/1‑94 (MQ=60)

                                                                   GGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGA                           <  SRR4302540.6074511/94‑1 (MQ=60)

                                                                    GTAACGCACACCGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAA                          <  SRR4302540.8010768/94‑1 (MQ=60)

                                                                    GTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAA                          <  SRR4302540.6242610/94‑1 (MQ=60)

                                                                     TAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAA                         >  SRR4302540.4664807/1‑94 (MQ=60)

                                                                     TAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAA                         >  SRR4302540.4664806/1‑94 (MQ=60)

                                                                      AACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAAC                        >  SRR4302540.2470028/1‑94 (MQ=60)

                                                                      AACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAAC                        >  SRR4302540.4548940/1‑94 (MQ=60)

                                                                       ACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACC                       >  SRR4302540.4816889/1‑94 (MQ=60)

                                                                        CGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTTGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCA                      <  SRR4302540.9613405/94‑1 (MQ=60)

                                                                        CGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCA                      <  SRR4302540.1135404/94‑1 (MQ=60)

                                                                        CGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCA                      <  SRR4302540.2740699/94‑1 (MQ=60)

                                                                          CACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACT                    <  SRR4302540.434356/94‑1 (MQ=60)

                                                                           ACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCTGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACCT                   >  SRR4302540.1432505/1‑94 (MQ=60)

                                                                           ACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTT                   >  SRR4302540.3632954/1‑94 (MQ=60)

                                                                             AACGGCAACGACGCGATCTGTGGATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTA                 <  SRR4302540.4176096/94‑1 (MQ=60)

                                                                              ACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAA                >  SRR4302540.8328750/1‑94 (MQ=60)

                                                                              ACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAA                >  SRR4302540.8313378/1‑94 (MQ=60)

                                                                              ACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAA                >  SRR4302540.4264809/1‑94 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGTCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302540.1428403/94‑1 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302540.1492655/94‑1 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302540.8468107/94‑1 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302540.8519156/94‑1 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302540.1024924/94‑1 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302540.1539677/94‑1 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302540.6060946/94‑1 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302540.4294830/94‑1 (MQ=60)

                                                                                GTCAACGACGCGAGCTGTGAATTCAATCGGCGGTGGTTCACCGGTCAGCAGACGCGCACGGTCGCGCAGCCACTCAATGGAAACCACTTTAATG              <  SRR4302540.6938542/94‑1 (MQ=60)

                                                                                GGCACCGACTCGATCTGTGAATTCAATCGGCTGTGGTGCCCCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATG              <  SRR4302540.6938545/94‑1 (MQ=60)

                                                                                GGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATG              <  SRR4302540.9026088/94‑1 (MQ=60)

                                                                                 GCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGC             <  SRR4302540.5182102/94‑1 (MQ=60)

                                                                                  CAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGGC            >  SRR4302540.8376420/1‑94 (MQ=60)

                                                                                  CAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCC            >  SRR4302540.8376421/1‑94 (MQ=60)

                                                                                  CAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCC            >  SRR4302540.6915431/1‑94 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCATCCGCGCACGTTCGCGCAGCACCTCAATGGAAACCCCTTTCATCCCC           >  SRR4302540.2520848/1‑94 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           >  SRR4302540.2520847/1‑94 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           <  SRR4302540.7493895/94‑1 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           <  SRR4302540.336975/94‑1 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           >  SRR4302540.3796477/1‑94 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           >  SRR4302540.6191178/1‑94 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           <  SRR4302540.8153590/94‑1 (MQ=60)

                                                                                    ACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCG          <  SRR4302540.7986861/94‑1 (MQ=60)

                                                                                      GACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGACACCACTTTAATGCCCGCT        >  SRR4302540.3906208/1‑94 (MQ=60)

                                                                                      GACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCT        >  SRR4302540.7038151/1‑94 (MQ=60)

                                                                                      GACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCT        >  SRR4302540.1343989/1‑94 (MQ=60)

                                                                                       ACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTT       >  SRR4302540.5459978/1‑94 (MQ=60)

                                                                                       ACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTT       <  SRR4302540.684664/94‑1 (MQ=60)

                                                                                        CGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTC      >  SRR4302540.8965294/1‑94 (MQ=60)

                                                                                           GATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTG   <  SRR4302540.4663512/94‑1 (MQ=60)

                                                                                           GATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTG   >  SRR4302540.469053/1‑94 (MQ=60)

                                                                                            ATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTGC  >  SRR4302540.5888018/1‑94 (MQ=60)

                                                                                            ATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTGC  >  SRR4302540.5248289/1‑94 (MQ=60)

AGTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTGC  >  NC_000913/647239‑647424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`