Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	647,331	G→A	D488D (GAC→GAT)	citF ←	citrate lyase alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	647,331	0	G	A	100.0%	913.9 / NA	247	D488D (GAC→GAT)	citF	citrate lyase alpha chain
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (120/127); total (120/127)

Only 100 of 251 total aligned reads displayed.

TTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTC  >  NC_000913/647241‑647420

 tCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCt                                                                                        >  2:1356457/1‑93 (MQ=255)

 tCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCt                                                                                        <  1:424458/93‑1 (MQ=255)

  cAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCtg                                                                                       >  2:2297666/1‑93 (MQ=255)

  cAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCtg                                                                                       <  2:2487952/93‑1 (MQ=255)

   aGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCtgt                                                                                      <  1:540552/93‑1 (MQ=255)

   aGGAAGCAGGTGCACGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCtgt                                                                                      <  1:1068532/93‑1 (MQ=255)

     gAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGa                                                                                    >  2:3081584/1‑93 (MQ=255)

        gCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAAtt                                                                                 >  1:4573449/1‑93 (MQ=255)

           ggTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCaa                                                                              <  1:4027004/93‑1 (MQ=255)

           ggTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCaa                                                                              <  2:774890/93‑1 (MQ=255)

           ggTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCaa                                                                              <  1:5542382/93‑1 (MQ=255)

            gTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAt                                                                             <  2:3471333/93‑1 (MQ=255)

            gTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAt                                                                             <  2:2512831/93‑1 (MQ=255)

            gTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAt                                                                             <  2:3926357/93‑1 (MQ=255)

            gTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAt                                                                             >  1:2782738/1‑93 (MQ=255)

             tGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATc                                                                            <  1:4179117/93‑1 (MQ=255)

              gCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCg                                                                           <  1:855730/93‑1 (MQ=255)

               cATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCgg                                                                          >  1:3944598/1‑93 (MQ=255)

                aTGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGc                                                                         >  1:744649/1‑93 (MQ=255)

                  ggCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCtg                                                                       >  1:5847205/1‑93 (MQ=255)

                    cTTATTCCTTCCCCTTATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCtgtg                                                                     <  1:15737/93‑1 (MQ=255)

                     ttATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTgg                                                                    >  2:1521041/1‑93 (MQ=255)

                     ttATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTgg                                                                    >  2:4195721/1‑93 (MQ=255)

                      tATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGt                                                                   <  1:6187419/93‑1 (MQ=255)

                      tATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGt                                                                   >  2:5296653/1‑93 (MQ=255)

                      tATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGt                                                                   >  1:3706703/1‑93 (MQ=255)

                       aTTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGtt                                                                  >  1:3838909/1‑93 (MQ=255)

                         tCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCa                                                                >  1:3027720/1‑93 (MQ=255)

                          ccTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCAc                                                               >  2:1789659/1‑93 (MQ=255)

                           cTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCAcc                                                              <  2:3321612/93‑1 (MQ=255)

                           cTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCAcc                                                              <  2:1587265/93‑1 (MQ=255)

                           cTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCAcc                                                              <  2:237932/93‑1 (MQ=255)

                            ttCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCg                                                             >  2:1408749/1‑93 (MQ=255)

                            ttCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCg                                                             >  2:3117580/1‑93 (MQ=255)

                            ttCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCg                                                             >  2:5946201/1‑93 (MQ=255)

                              ccccTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGt                                                           <  2:4530388/91‑1 (MQ=255)

                              cACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGt                                                           <  1:380590/93‑1 (MQ=255)

                               cccTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTc                                                          <  1:3240770/92‑1 (MQ=255)

                                ccTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTca                                                         >  1:3601726/1‑93 (MQ=255)

                                ccTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTca                                                         <  1:4871805/93‑1 (MQ=255)

                                 cTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCATCGGTcag                                                        >  2:4376444/1‑93 (MQ=255)

                                 cTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTcag                                                        <  2:1618759/93‑1 (MQ=255)

                                  tGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTcagc                                                       >  1:4728716/1‑93 (MQ=255)

                                  tGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTcagc                                                       >  2:4688704/1‑93 (MQ=255)

                                     tGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGa                                                    >  2:5575862/1‑93 (MQ=255)

                                     tGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGa                                                    >  2:6087366/1‑93 (MQ=255)

                                     tGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGa                                                    <  1:2450961/93‑1 (MQ=255)

                                        acaacaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGAcgc                                                 <  2:3747325/93‑1 (MQ=255)

                                         caacaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGAcgcg                                                >  1:497738/1‑93 (MQ=255)

                                         caacaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGAcgcg                                                >  1:605768/1‑93 (MQ=255)

                                           acaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCa                                              >  2:3609238/1‑93 (MQ=255)

                                           acaTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGATGTGGCTCACCGGGCAGCAGAAGCGCa                                              >  1:3020026/1‑93 (MQ=255)

                                             aTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACg                                            >  2:901618/1‑93 (MQ=255)

                                             aTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACg                                            <  1:2270946/93‑1 (MQ=255)

                                             aTCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACg                                            >  1:155605/1‑93 (MQ=255)

                                              tCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGt                                           <  1:5519399/93‑1 (MQ=255)

                                               cGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGtt                                          <  1:5328035/93‑1 (MQ=255)

                                                taTCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTc                                         <  1:3565521/92‑1 (MQ=255)

                                                 aTCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTcg                                        >  2:2820262/1‑93 (MQ=255)

                                                 aTCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTcg                                        <  2:5079723/93‑1 (MQ=255)

                                                  tCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTcgc                                       <  2:194494/93‑1 (MQ=255)

                                                    aCCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCgcgc                                     >  1:4504976/1‑93 (MQ=255)

                                                     ccGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCa                                    >  2:5987701/1‑93 (MQ=255)

                                                     ccGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCa                                    <  2:5517387/93‑1 (MQ=255)

                                                      cGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAg                                   >  1:2866960/1‑93 (MQ=255)

                                                       gAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGc                                  >  2:981828/1‑93 (MQ=255)

                                                         gCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCa                                <  2:2017738/93‑1 (MQ=255)

                                                         gCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCa                                >  1:5821230/1‑93 (MQ=255)

                                                         gCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCa                                >  1:5766694/1‑93 (MQ=255)

                                                          ccATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCAc                               <  2:876533/93‑1 (MQ=255)

                                                          ccATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCAc                               <  1:1354719/93‑1 (MQ=255)

                                                            aTCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTc                             <  2:697961/93‑1 (MQ=255)

                                                             tCGCGTTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCa                                <  2:1504547/89‑1 (MQ=255)

                                                             tCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCa                            >  2:2755209/1‑93 (MQ=255)

                                                             tCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCa                            >  1:1798900/1‑93 (MQ=255)

                                                              cgcgGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCaa                           <  1:1895468/93‑1 (MQ=255)

                                                               gcgGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAAt                          <  2:5006004/93‑1 (MQ=255)

                                                                 ggTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATgg                        >  1:6273867/1‑93 (MQ=255)

                                                                  gTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGa                       >  1:3544852/1‑93 (MQ=255)

                                                                  gTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGa                       >  1:3135193/1‑93 (MQ=255)

                                                                      cGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAAcc                   <  1:5171414/93‑1 (MQ=255)

                                                                      cGCACAACGGCAACGACGCGATCTGTGAATTCAATCGACTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAAcc                   <  1:5924885/93‑1 (MQ=255)

                                                                       gCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCa                  >  2:110223/1‑93 (MQ=255)

                                                                        cacaACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCAc                 >  1:127617/1‑93 (MQ=255)

                                                                         acaACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACt                >  1:1091697/1‑93 (MQ=255)

                                                                          caACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACtt               <  1:511672/93‑1 (MQ=255)

                                                                          caACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACtt               <  2:3782604/93‑1 (MQ=255)

                                                                             cGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTaa            <  2:4772650/93‑1 (MQ=255)

                                                                              ggCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAt           <  1:962025/93‑1 (MQ=255)

                                                                               gCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATg          <  2:1837576/93‑1 (MQ=255)

                                                                                 aaCGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGcc        >  1:2595579/1‑93 (MQ=255)

                                                                                 aaCGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGcc        <  1:2630433/93‑1 (MQ=255)

                                                                                  acgacgCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGccc       <  1:3403594/93‑1 (MQ=255)

                                                                                  acgacgCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGccc       >  1:2106282/1‑93 (MQ=255)

                                                                                    gacgCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGc     <  1:3637748/93‑1 (MQ=255)

                                                                                      cgcgATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCtt   >  2:5764821/1‑93 (MQ=255)

                                                                                      cgcgATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCtt   >  2:2944629/1‑93 (MQ=255)

                                                                                      cgcgATCTGTGAATTCAATCGGCTGTGGTCCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCtt   <  2:6192317/93‑1 (MQ=255)

                                                                                       gcgATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTc  >  1:3075237/1‑93 (MQ=255)

                                                                                       gcgATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTc  >  1:1632174/1‑93 (MQ=255)

TTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTC  >  NC_000913/647241‑647420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTGC  >  NC_000913/647239‑647424

AGTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGAT                                                                                              <  SRR4302533.2610706/94‑1 (MQ=60)

 GTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATC                                                                                             <  SRR4302533.5286347/94‑1 (MQ=60)

  TTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCCCCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCT                                                                                            <  SRR4302533.5994130/94‑1 (MQ=60)

  TTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCT                                                                                            >  SRR4302533.3138543/1‑94 (MQ=60)

   TCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTG                                                                                           <  SRR4302533.426994/94‑1 (MQ=60)

     AGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTG                                                                                         <  SRR4302533.3609189/94‑1 (MQ=60)

     AGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTG                                                                                         <  SRR4302533.2784420/94‑1 (MQ=60)

     AGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTG                                                                                         <  SRR4302533.5390653/94‑1 (MQ=60)

     AGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTG                                                                                         <  SRR4302533.543770/94‑1 (MQ=60)

     AGGAAGCAGGTGCACGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTG                                                                                         <  SRR4302533.1074823/94‑1 (MQ=60)

        AAGCAGGTTCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAAT                                                                                      <  SRR4302533.2568328/94‑1 (MQ=60)

        AAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAAT                                                                                      <  SRR4302533.3054231/94‑1 (MQ=60)

         AGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATT                                                                                     >  SRR4302533.4601166/1‑94 (MQ=60)

           CAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCA                                                                                   <  SRR4302533.3824579/94‑1 (MQ=60)

             GGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAT                                                                                 <  SRR4302533.5575856/94‑1 (MQ=60)

             GGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAT                                                                                 <  SRR4302533.5867470/94‑1 (MQ=60)

             GGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAT                                                                                 >  SRR4302533.2799834/1‑94 (MQ=60)

             GGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAAT                                                                                 <  SRR4302533.4051518/94‑1 (MQ=60)

              GTGCATGGCTTATTCCTTCCCCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATC                                                                                <  SRR4302533.1550902/94‑1 (MQ=60)

              GTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATC                                                                                >  SRR4302533.5545343/1‑94 (MQ=60)

              GTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATC                                                                                >  SRR4302533.2080444/1‑94 (MQ=60)

               TGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCG                                                                               <  SRR4302533.3986615/94‑1 (MQ=60)

               TGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCG                                                                               <  SRR4302533.4204431/94‑1 (MQ=60)

                GCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGG                                                                              >  SRR4302533.3968660/1‑94 (MQ=60)

                GCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGG                                                                              <  SRR4302533.860745/94‑1 (MQ=60)

                 CATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGC                                                                             >  SRR4302533.749032/1‑94 (MQ=60)

                  ATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCT                                                                            >  SRR4302533.3052641/1‑94 (MQ=60)

                  ATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCT                                                                            >  SRR4302533.1631105/1‑94 (MQ=60)

                   TGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTG                                                                           >  SRR4302533.5882554/1‑94 (MQ=60)

                    GGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGT                                                                          >  SRR4302533.3855532/1‑94 (MQ=60)

                      CTTATTCCTTCCCCTTATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGG                                                                        <  SRR4302533.15848/94‑1 (MQ=60)

                      CTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGG                                                                        >  SRR4302533.3906809/1‑94 (MQ=60)

                      CTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGG                                                                        <  SRR4302533.819391/94‑1 (MQ=60)

                       TTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGT                                                                       >  SRR4302533.3729469/1‑94 (MQ=60)

                       TTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGT                                                                       >  SRR4302533.4104522/1‑94 (MQ=60)

                        TATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTT                                                                      >  SRR4302533.3862361/1‑94 (MQ=60)

                        TATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTT                                                                      <  SRR4302533.6224558/94‑1 (MQ=60)

                         ATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTC                                                                     >  SRR4302533.3199632/1‑94 (MQ=60)

                         ATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGCTC                                                                     >  SRR4302533.3043649/1‑94 (MQ=60)

                          TTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCA                                                                    >  SRR4302533.3046249/1‑94 (MQ=60)

                           TCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCAC                                                                   >  SRR4302533.3515602/1‑94 (MQ=60)

                            CCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACC                                                                  >  SRR4302533.4015513/1‑94 (MQ=60)

                            CCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACC                                                                  <  SRR4302533.5749916/94‑1 (MQ=60)

                             CTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCG                                                                 >  SRR4302533.6135469/1‑94 (MQ=60)

                              TTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGG                                                                >  SRR4302533.357442/1‑94 (MQ=60)

                               TCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGT                                                               >  SRR4302533.3297409/1‑94 (MQ=60)

                                CACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTC                                                              >  SRR4302533.5092541/1‑94 (MQ=60)

                                CACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTC                                                              <  SRR4302533.382882/94‑1 (MQ=60)

                                 CCCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCA                                                             <  SRR4302533.533587/94‑1 (MQ=60)

                                 CCCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCA                                                             <  SRR4302533.3260739/94‑1 (MQ=60)

                                 ACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCA                                                             <  SRR4302533.694077/94‑1 (MQ=60)

                                 ACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCA                                                             >  SRR4302533.3623890/1‑94 (MQ=60)

                                  CCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAG                                                            <  SRR4302533.4901325/94‑1 (MQ=60)

                                  CCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAG                                                            <  SRR4302533.426867/94‑1 (MQ=60)

                                   CTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGC                                                           >  SRR4302533.4757384/1‑94 (MQ=60)

                                   CTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGC                                                           >  SRR4302533.3659878/1‑94 (MQ=60)

                                       TGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGAC                                                       <  SRR4302533.4596185/94‑1 (MQ=60)

                                       TGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGAC                                                       <  SRR4302533.5716936/94‑1 (MQ=60)

                                       TGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGAC                                                       <  SRR4302533.2466091/94‑1 (MQ=60)

                                        GCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACG                                                      <  SRR4302533.5201538/94‑1 (MQ=60)

                                        GCACAACATCGAGCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACG                                                      <  SRR4302533.1881551/94‑1 (MQ=60)

                                         CACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGC                                                     <  SRR4302533.2961054/94‑1 (MQ=60)

                                          ACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCG                                                    >  SRR4302533.500705/1‑94 (MQ=60)

                                          ACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCG                                                    >  SRR4302533.609328/1‑94 (MQ=60)

                                           CAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGC                                                   <  SRR4302533.3660924/94‑1 (MQ=60)

                                            AACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGATGTGGCTCACCGGGCAGCAGAAGCGCA                                                  >  SRR4302533.3038513/1‑94 (MQ=60)

                                             ACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCAC                                                 >  SRR4302533.6266815/1‑94 (MQ=60)

                                              CATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACG                                                >  SRR4302533.156597/1‑94 (MQ=60)

                                               ATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGT                                               <  SRR4302533.2284998/94‑1 (MQ=60)

                                                TCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTT                                              <  SRR4302533.5552724/94‑1 (MQ=60)

                                                 CGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTC                                             <  SRR4302533.5359963/94‑1 (MQ=60)

                                                 CGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTC                                             <  SRR4302533.2194057/94‑1 (MQ=60)

                                                  TATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCG                                            <  SRR4302533.3587494/94‑1 (MQ=60)

                                                  GATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCG                                            >  SRR4302533.5457698/1‑94 (MQ=60)

                                                  GATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCG                                            >  SRR4302533.2910209/1‑94 (MQ=60)

                                                  GATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCG                                            <  SRR4302533.4677668/94‑1 (MQ=60)

                                                     CACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCCGATGCGCACGctcgcca                                         >  SRR4302533.2796090/1‑87 (MQ=60)

                                                     CACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGC                                         >  SRR4302533.1095268/1‑94 (MQ=60)

                                                     CACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGC                                         >  SRR4302533.4532266/1‑94 (MQ=60)

                                                      CCCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCA                                        <  SRR4302533.1842298/94‑1 (MQ=60)

                                                      ACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCA                                        <  SRR4302533.1243371/94‑1 (MQ=60)

                                                      ACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCA                                        >  SRR4302533.4367883/1‑94 (MQ=60)

                                                       CCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAG                                       >  SRR4302533.2884597/1‑94 (MQ=60)

                                                        CGAGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGC                                      >  SRR4302533.6321045/1‑94 (MQ=60)

                                                          AGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGGAGCCA                                    >  SRR4302533.5872115/1‑94 (MQ=60)

                                                          AGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCA                                    >  SRR4302533.5801508/1‑94 (MQ=60)

                                                          AGCCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCA                                    >  SRR4302533.5856393/1‑94 (MQ=60)

                                                            CCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACT                                  <  SRR4302533.1362861/94‑1 (MQ=60)

                                                            CCATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACT                                  <  SRR4302533.1180510/94‑1 (MQ=60)

                                                              ATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCA                                >  SRR4302533.1809846/1‑94 (MQ=60)

                                                              ATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCA                                <  SRR4302533.3520835/94‑1 (MQ=60)

                                                              ATCGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCA                                <  SRR4302533.555771/94‑1 (MQ=60)

                                                              ATCGCGGTAACGCACAAAGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACata                                >  SRR4302533.1513618/1‑91 (MQ=60)

                                                                CGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAAT                              <  SRR4302533.1907037/94‑1 (MQ=60)

                                                                CGCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAAT                              <  SRR4302533.941950/94‑1 (MQ=60)

                                                                 GCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATG                             <  SRR4302533.2550727/94‑1 (MQ=60)

                                                                 GCGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATG                             <  SRR4302533.3831927/94‑1 (MQ=60)

                                                                  CGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGG                            >  SRR4302533.6311339/1‑94 (MQ=60)

                                                                  CGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGG                            >  SRR4302533.2283568/1‑94 (MQ=60)

                                                                  CGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGG                            >  SRR4302533.4844099/1‑94 (MQ=60)

                                                                  CGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGG                            >  SRR4302533.927213/1‑94 (MQ=60)

                                                                  CGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGG                            <  SRR4302533.5811792/94‑1 (MQ=60)

                                                                  CGGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGG                            <  SRR4302533.3692252/94‑1 (MQ=60)

                                                                   GGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGA                           >  SRR4302533.5310948/1‑94 (MQ=60)

                                                                   GGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGA                           >  SRR4302533.172627/1‑94 (MQ=60)

                                                                   GGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGA                           >  SRR4302533.3154473/1‑94 (MQ=60)

                                                                   GGTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGA                           >  SRR4302533.3566701/1‑94 (MQ=60)

                                                                    GTAACGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAA                          <  SRR4302533.6295396/94‑1 (MQ=60)

                                                                        CGCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCA                      <  SRR4302533.5202621/94‑1 (MQ=60)

                                                                        CGCACAACGGCAACGACGCGATCTGTGAATTCAATCGACTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCA                      <  SRR4302533.5960704/94‑1 (MQ=60)

                                                                         GCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCAC                     >  SRR4302533.5108326/1‑94 (MQ=60)

                                                                         GCACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCAC                     >  SRR4302533.128415/1‑94 (MQ=60)

                                                                          CACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACT                    >  SRR4302533.1098149/1‑94 (MQ=60)

                                                                          CACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACT                    <  SRR4302533.1339886/94‑1 (MQ=60)

                                                                          CACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGAGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACT                    >  SRR4302533.1569813/1‑94 (MQ=60)

                                                                           ACAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTT                   <  SRR4302533.2616109/94‑1 (MQ=60)

                                                                            CAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTT                  <  SRR4302533.514712/94‑1 (MQ=60)

                                                                            CAACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTT                  <  SRR4302533.2817844/94‑1 (MQ=60)

                                                                             AACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACNACTNTA                 >  SRR4302533.1615309/1‑94 (MQ=60)

                                                                              ACGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAA                <  SRR4302533.3169301/94‑1 (MQ=60)

                                                                               CGGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302533.4980740/94‑1 (MQ=60)

                                                                               CGGCAACGACGATATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAAT               <  SRR4302533.4817674/94‑1 (MQ=60)

                                                                                GGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATG              <  SRR4302533.967676/94‑1 (MQ=60)

                                                                                GGCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATC              >  SRR4302533.6022609/1‑94 (MQ=60)

                                                                                 GCAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGC             >  SRR4302533.4267321/1‑94 (MQ=60)

                                                                                  CAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCC            >  SRR4302533.2287606/1‑94 (MQ=60)

                                                                                  CAACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCC            >  SRR4302533.2611531/1‑94 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           <  SRR4302533.4574303/94‑1 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           >  SRR4302533.2119270/1‑94 (MQ=60)

                                                                                   AACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCC           <  SRR4302533.2646596/94‑1 (MQ=60)

                                                                                    ACGACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCG          <  SRR4302533.3424611/94‑1 (MQ=60)

                                                                                      GACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCT        <  SRR4302533.5916006/94‑1 (MQ=60)

                                                                                      GACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCT        <  SRR4302533.3660113/94‑1 (MQ=60)

                                                                                       ACGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTT       >  SRR4302533.3604362/1‑94 (MQ=60)

                                                                                        CGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTC      >  SRR4302533.3094110/1‑94 (MQ=60)

                                                                                        CGCGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTC      >  SRR4302533.1642061/1‑94 (MQ=60)

                                                                                          CGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCT    >  SRR4302533.4673315/1‑94 (MQ=60)

                                                                                          CGATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCT    >  SRR4302533.1058692/1‑94 (MQ=60)

                                                                                           GATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTG   >  SRR4302533.6114162/1‑94 (MQ=60)

                                                                                            ATCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTGC  >  SRR4302533.2398015/1‑94 (MQ=60)

AGTTCAGGAAGCAGGTGCATGGCTTATTCCTTCACCTGATGCACAACATCGATCACCGAGCCATCGCGGTAACGCACAACGGCAACGACGCGGTCTGTGAATTCAATCGGCTGTGGTTCACCGGTCAGCAGACGCGCACGTTCGCGCAGCCACTCAATGGAAACCACTTTAATGCCCGCTTCCTGC  >  NC_000913/647239‑647424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`