Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 766,372 C→T A389V (GCG→GTG)  hrsA → PTS system fructose specific IIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,3720CT100.0% 859.3 / NA 234A389V (GCG→GTG) hrsAPTS system fructose specific IIABC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (128/106);  total (128/106)

Only 100 of 243 total aligned reads displayed.

GCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA  >  NC_000913/766282‑766461
                                                                                          |                                                                                         
  gggCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCt                                                                                       >  2:5361237/1‑93 (MQ=255)
  gggCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCt                                                                                       >  1:9221460/1‑93 (MQ=255)
   gtctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTg                                                                                      <  2:6335090/91‑1 (MQ=255)
   ggCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTg                                                                                      >  1:5576444/1‑93 (MQ=255)
   ggCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTg                                                                                      >  2:7841723/1‑93 (MQ=255)
   ggCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTg                                                                                      >  1:8260116/1‑93 (MQ=255)
     ctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGa                                                                                    <  2:5632167/93‑1 (MQ=255)
     ctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGa                                                                                    >  2:10007251/1‑93 (MQ=255)
     ctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGa                                                                                    >  2:1582711/1‑93 (MQ=255)
      tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                   >  2:9150393/1‑93 (MQ=255)
      tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                   <  1:6448380/93‑1 (MQ=255)
      tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                   >  2:4577131/1‑93 (MQ=255)
      tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAc                                                                                   >  1:2168436/1‑93 (MQ=255)
        ctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtt                                                                                 >  1:6665179/1‑93 (MQ=255)
           ggAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtttgc                                                                              >  1:9569205/1‑90 (MQ=255)
                 ttGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAAc                                                                        <  1:7306330/93‑1 (MQ=255)
                  tGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACa                                                                       <  1:27636/93‑1 (MQ=255)
                  tGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACa                                                                       <  1:4555015/93‑1 (MQ=255)
                   gATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAGTTGCCGCCCACAt                                                                      >  2:4344728/1‑93 (MQ=255)
                   gATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACAt                                                                      <  1:4473927/93‑1 (MQ=255)
                      ggTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGAt                                                                   <  2:2006701/93‑1 (MQ=255)
                      ggTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGAt                                                                   <  1:3945136/93‑1 (MQ=255)
                       gTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATc                                                                  <  2:13243/93‑1 (MQ=255)
                         aCCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCgg                                                                >  1:5850126/1‑93 (MQ=255)
                          ccGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGc                                                               <  2:9823829/93‑1 (MQ=255)
                            cgTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTc                                                             <  1:2324452/92‑1 (MQ=255)
                             gTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTcc                                                            <  2:278467/93‑1 (MQ=255)
                              tGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCg                                                           >  1:9012849/1‑93 (MQ=255)
                               gCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCgg                                                          >  1:380464/1‑93 (MQ=255)
                                 ccgcgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGt                                                        <  1:2431061/92‑1 (MQ=255)
                                   gcgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGttt                                                      >  2:5152886/1‑93 (MQ=255)
                                   gcgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGttt                                                      >  2:2969098/1‑93 (MQ=255)
                                   gcgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGgtt                                                      >  1:522969/1‑93 (MQ=255)
                                    cgGCCTATACCGCCTATTCTCTGGCAGATAACCCGGCGTTAGCGCCAGGCTTTGTGGCTGGATTTGCCGCCAACATGATCGGCTCCGGGTTtc                                                     <  1:3280506/93‑1 (MQ=255)
                                      gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGtttttt                                                   >  2:8120903/1‑90 (MQ=255)
                                      gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGGGGCTGGCCTTGCCGCACACATGATCCGCTCCGGGGTtctc                                                   >  1:6551920/1‑93 (MQ=255)
                                       ccTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCg                                                  >  2:2327502/1‑93 (MQ=255)
                                        cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                 <  1:8596540/93‑1 (MQ=255)
                                        cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCgg                                                 >  2:4460620/1‑93 (MQ=255)
                                         tataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgc                                                >  2:1773847/1‑93 (MQ=255)
                                         tataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgc                                                >  1:8083488/1‑93 (MQ=255)
                                          ataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgcg                                               >  2:273602/1‑93 (MQ=255)
                                          ataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgcg                                               >  1:6502913/1‑93 (MQ=255)
                                          ataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgcg                                               <  2:7860897/93‑1 (MQ=255)
                                           taCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgcgc                                              >  2:5277813/1‑93 (MQ=255)
                                           taCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgcgc                                              >  2:1208225/1‑93 (MQ=255)
                                           taCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgcgc                                              >  2:9710647/1‑93 (MQ=255)
                                              cGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGt                                           >  1:6617385/1‑93 (MQ=255)
                                              cGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGt                                           <  1:4110354/93‑1 (MQ=255)
                                               gCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTc                                          >  2:7032692/1‑93 (MQ=255)
                                                ccTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCg                                         >  1:2606833/1‑93 (MQ=255)
                                                 cTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACCTGATCGGCTCCGGGTTTCTCGGCGCGGTCGt                                        <  2:8608095/93‑1 (MQ=255)
                                                 cTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGt                                        >  1:7929042/1‑93 (MQ=255)
                                                 cTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGGCGt                                        >  2:850748/1‑93 (MQ=255)
                                                  tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGtt                                       >  1:9830988/1‑93 (MQ=255)
                                                    ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                     <  2:2676162/93‑1 (MQ=255)
                                                     tctcTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCCGGGTTCTCGGCGCGGTCGTTggg                                    >  1:131310/1‑92 (MQ=255)
                                                         tGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGAt                                <  2:7355663/93‑1 (MQ=255)
                                                         tGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGAt                                <  2:4715314/93‑1 (MQ=255)
                                                           gCAGATAAACCGGCGTTAGCGCCTGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTg                              <  2:5533817/93‑1 (MQ=255)
                                                            cAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGa                             <  1:5655846/93‑1 (MQ=255)
                                                             aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAt                            <  1:373537/93‑1 (MQ=255)
                                                             aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAt                            >  1:7785022/1‑93 (MQ=255)
                                                              gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATa                           >  2:8915334/1‑93 (MQ=255)
                                                              gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATa                           >  2:9468755/1‑93 (MQ=255)
                                                              gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGGTTGATa                           >  1:3169029/1‑93 (MQ=255)
                                                               aTAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAg                          <  2:6721416/93‑1 (MQ=255)
                                                                tAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGc                         <  2:8365226/93‑1 (MQ=255)
                                                                  aaCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCg                       <  2:2954184/93‑1 (MQ=255)
                                                                   aCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCgg                      <  2:8851082/93‑1 (MQ=255)
                                                                   aCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCgg                      <  1:1836098/93‑1 (MQ=255)
                                                                   aCCGGCGTAAACGCCAGGCTTGGTAGCTGTACTTTCCCCCAACATTATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGAATTGATAGCCgg                      <  2:190036/93‑1 (MQ=255)
                                                                     cGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGtt                    >  2:3800657/1‑93 (MQ=255)
                                                                        cGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACt                 <  1:8717204/93‑1 (MQ=255)
                                                                          ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg               >  1:105828/1‑93 (MQ=255)
                                                                          ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg               >  1:5951734/1‑93 (MQ=255)
                                                                           tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa              <  1:3698052/93‑1 (MQ=255)
                                                                           tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa              <  1:5414098/93‑1 (MQ=255)
                                                                           tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa              <  1:1229262/93‑1 (MQ=255)
                                                                           tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa              <  2:7984220/93‑1 (MQ=255)
                                                                           tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa              <  2:3870144/93‑1 (MQ=255)
                                                                            aGCGCGAGGCTTTGTGGCTGGACTGGCCGCGAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAt             <  2:4047555/93‑1 (MQ=255)
                                                                               gcCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTCATTGATggc          >  2:3456784/1‑91 (MQ=255)
                                                                                ccAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATgcgc         >  1:9499471/1‑93 (MQ=255)
                                                                                 cAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCt        <  1:3077891/93‑1 (MQ=255)
                                                                                 cAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCt        >  1:3051775/1‑93 (MQ=255)
                                                                                 cAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCt        <  1:6251055/93‑1 (MQ=255)
                                                                                  aGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGGGCTg       >  2:3697132/1‑93 (MQ=255)
                                                                                  aGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTg       <  2:4889243/93‑1 (MQ=255)
                                                                                  aGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTg       >  1:699685/1‑93 (MQ=255)
                                                                                  aGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTg       >  1:6191198/1‑93 (MQ=255)
                                                                                   ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTgg      <  1:7066856/93‑1 (MQ=255)
                                                                                   ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTgg      >  2:9313551/1‑93 (MQ=255)
                                                                                   ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGGTACTTGCTGCGCTgg      >  2:7441239/1‑93 (MQ=255)
                                                                                     cTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGt    <  2:5721130/93‑1 (MQ=255)
                                                                                      tttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTg   >  1:5140622/1‑93 (MQ=255)
                                                                                      tttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTg   >  2:3008652/1‑93 (MQ=255)
                                                                                       ttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGa  >  1:2757242/1‑93 (MQ=255)
                                                                                       ttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGa  <  2:104124/93‑1 (MQ=255)
                                                                                       ttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGAGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGa  >  1:8760561/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA  >  NC_000913/766282‑766461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAAAAA  >  NC_000913/766279‑766465
                                                                                             |                                                                                             
GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGT                                                                                               <  SRR4302540.2966199/94‑1 (MQ=60)
GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGT                                                                                               <  SRR4302540.5312397/94‑1 (MQ=60)
GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGT                                                                                               <  SRR4302540.8142633/94‑1 (MQ=60)
  GGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGG                                                                                             <  SRR4302540.5562296/94‑1 (MQ=60)
  GGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGG                                                                                             <  SRR4302540.3359055/94‑1 (MQ=60)
  GGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGG                                                                                             <  SRR4302540.3548865/94‑1 (MQ=60)
    CGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCT                                                                                           >  SRR4302540.9332047/1‑94 (MQ=60)
    CGGGCTGCTCGGAATTTTGATGGTACCGGTCCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCT                                                                                           <  SRR4302540.5568116/94‑1 (MQ=60)
     GGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTG                                                                                          >  SRR4302540.1247982/1‑94 (MQ=60)
     GGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTG                                                                                          >  SRR4302540.8356102/1‑94 (MQ=60)
     GGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTG                                                                                          >  SRR4302540.5637840/1‑94 (MQ=60)
     GGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTG                                                                                          >  SRR4302540.8356103/1‑94 (MQ=60)
      GGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGG                                                                                         <  SRR4302540.513069/94‑1 (MQ=60)
      GGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCAATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGG                                                                                         >  SRR4302540.8733664/1‑94 (MQ=60)
        CTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAC                                                                                       >  SRR4302540.2191818/1‑94 (MQ=60)
         TGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACT                                                                                      <  SRR4302540.6520161/94‑1 (MQ=60)
          GCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTT                                                                                     >  SRR4302540.6738850/1‑94 (MQ=60)
             CGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTtgc                                                                                  >  SRR4302540.9682272/1‑91 (MQ=60)
             CGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCC                                                                                  <  SRR4302540.4514367/94‑1 (MQ=60)
              GGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCG                                                                                 <  SRR4302540.6853681/94‑1 (MQ=60)
                  TTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAA                                                                             <  SRR4302540.6664539/94‑1 (MQ=60)
                    TTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACC                                                                           >  SRR4302540.3153550/1‑94 (MQ=60)
                    TTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACA                                                                           <  SRR4302540.7387897/94‑1 (MQ=60)
                     TGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACAT                                                                          <  SRR4302540.27933/94‑1 (MQ=60)
                     TGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACAT                                                                          <  SRR4302540.4604265/94‑1 (MQ=60)
                      GATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATG                                                                         >  SRR4302540.4739370/1‑94 (MQ=60)
                      GATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATG                                                                         <  SRR4302540.4522453/94‑1 (MQ=60)
                        TGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGAT                                                                       >  SRR4302540.3101042/1‑94 (MQ=60)
                         GGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATC                                                                      <  SRR4302540.3987738/94‑1 (MQ=60)
                         GGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATC                                                                      >  SRR4302540.278543/1‑94 (MQ=60)
                           TACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGG                                                                    >  SRR4302540.5914825/1‑94 (MQ=60)
                            ACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGC                                                                   <  SRR4302540.7882846/94‑1 (MQ=60)
                            ACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCCGC                                                                   >  SRR4302540.6551242/1‑94 (MQ=60)
                             CCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCT                                                                  <  SRR4302540.3229548/94‑1 (MQ=60)
                             CCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCC                                                                  >  SRR4302540.6733038/1‑94 (MQ=60)
                               CGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCC                                                                <  SRR4302540.2349484/94‑1 (MQ=60)
                                GTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCG                                                               >  SRR4302540.9120976/1‑94 (MQ=60)
                                 TGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGG                                                              >  SRR4302540.384404/1‑94 (MQ=60)
                                   CTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCTGGGT                                                            <  SRR4302540.3858192/94‑1 (MQ=60)
                                   CTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGT                                                            >  SRR4302540.10027264/1‑94 (MQ=60)
                                   CTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGT                                                            <  SRR4302540.3869167/94‑1 (MQ=60)
                                    TCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTT                                                           <  SRR4302540.2457251/94‑1 (MQ=60)
                                    TCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTT                                                           <  SRR4302540.9036604/94‑1 (MQ=60)
                                    TCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTT                                                           <  SRR4302540.2569031/94‑1 (MQ=60)
                                    CCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTT                                                           <  SRR4302540.2457252/94‑1 (MQ=60)
                                     CGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTT                                                          >  SRR4302540.528602/1‑94 (MQ=60)
                                       CGGCCTATACCGCCTATTCTCTGGCAGATAACCCGGCGTTAGCGCCAGGCTTTGTGGCTGGATTTGCCGCCAACATGATCGGCTCCGGGTTTCT                                                        <  SRR4302540.3316341/94‑1 (MQ=60)
                                        GGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTC                                                       >  SRR4302540.6624588/1‑94 (MQ=60)
                                        GGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTC                                                       >  SRR4302540.3008300/1‑94 (MQ=60)
                                        GGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTC                                                       >  SRR4302540.5755871/1‑94 (MQ=60)
                                        GGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGGGGCTGGCCTTGCCGCACACATGATCCGCTCCGGGGTTCTC                                                       >  SRR4302540.6624586/1‑94 (MQ=60)
                                         GCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCG                                                      <  SRR4302540.884342/94‑1 (MQ=60)
                                         GCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCG                                                      <  SRR4302540.2659595/94‑1 (MQ=60)
                                           CTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGCCCTGCCGCCGACATTATCGGCTCCGGGTTTCTCcgg                                                    >  SRR4302540.3795627/1‑91 (MQ=60)
                                           CTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGC                                                    >  SRR4302540.8177301/1‑94 (MQ=60)
                                           CTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGC                                                    <  SRR4302540.8696466/94‑1 (MQ=60)
                                           CTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGC                                                    >  SRR4302540.1817934/1‑94 (MQ=60)
                                            TATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCG                                                   >  SRR4302540.6575199/1‑94 (MQ=60)
                                             ATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGC                                                  <  SRR4302540.4050282/94‑1 (MQ=60)
                                              TACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCG                                                 <  SRR4302540.4591652/94‑1 (MQ=60)
                                               ACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGG                                                >  SRR4302540.2882127/1‑94 (MQ=60)
                                                CCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGT                                               >  SRR4302540.6690668/1‑94 (MQ=60)
                                                 CGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCATGCTTTGTGGCTGGACTTGCCGGCAACATGATCGGCTCCGGGTTTCTCAGCGCGGGC                                              >  SRR4302540.976433/1‑94 (MQ=60)
                                                 CGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTC                                              >  SRR4302540.8477619/1‑94 (MQ=60)
                                                 CGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTC                                              <  SRR4302540.4154722/94‑1 (MQ=60)
                                                 CGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTC                                              >  SRR4302540.9826940/1‑94 (MQ=60)
                                                  GCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCG                                             >  SRR4302540.2634967/1‑94 (MQ=60)
                                                  GCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCG                                             >  SRR4302540.5454601/1‑94 (MQ=60)
                                                   CCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGT                                            >  SRR4302540.8021454/1‑94 (MQ=60)
                                                   CCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGT                                            >  SRR4302540.9822417/1‑94 (MQ=60)
                                                    CTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTT                                           >  SRR4302540.9945966/1‑94 (MQ=60)
                                                    CTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTT                                           <  SRR4302540.6160356/94‑1 (MQ=60)
                                                    CTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTTTCGGCGCGGTCGTT                                           >  SRR4302540.8096701/1‑94 (MQ=60)
                                                     TATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTG                                          >  SRR4302540.7565187/1‑94 (MQ=60)
                                                      ATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGGCGTTGG                                         >  SRR4302540.886543/1‑94 (MQ=60)
                                                       TTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCCGGGTTCTCGGCGCGGTCGTTGGG                                        >  SRR4302540.132689/1‑94 (MQ=60)
                                                         CTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGG                                      >  SRR4302540.8519453/1‑94 (MQ=60)
                                                            TGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATT                                   <  SRR4302540.252650/94‑1 (MQ=60)
                                                             GGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTG                                  <  SRR4302540.5823048/94‑1 (MQ=60)
                                                             GGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTG                                  >  SRR4302540.6533186/1‑94 (MQ=60)
                                                              GCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGA                                 >  SRR4302540.6501932/1‑94 (MQ=60)
                                                               CAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAT                                <  SRR4302540.5718123/94‑1 (MQ=60)
                                                               CAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAT                                >  SRR4302540.7876020/1‑94 (MQ=60)
                                                               CAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAT                                <  SRR4302540.694814/94‑1 (MQ=60)
                                                               CAGATAAACCGGCGTCAGCGTCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAT                                <  SRR4302540.8135957/94‑1 (MQ=60)
                                                                AGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCTGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATA                               <  SRR4302540.2305276/94‑1 (MQ=60)
                                                                AGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATA                               <  SRR4302540.377402/94‑1 (MQ=60)
                                                                AGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGGTTGATA                               >  SRR4302540.3203479/1‑94 (MQ=60)
                                                                    AAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCG                           <  SRR4302540.1174105/94‑1 (MQ=60)
                                                                    AAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCG                           <  SRR4302540.5675626/94‑1 (MQ=60)
                                                                      ACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGT                         <  SRR4302540.1856203/94‑1 (MQ=60)
                                                                       CCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTT                        >  SRR4302540.3765219/1‑94 (MQ=60)
                                                                         GGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTAC                      <  SRR4302540.9408546/94‑1 (MQ=60)
                                                                         GGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGGCGTTGGCGGATTGCTAGCCGGTTAC                      >  SRR4302540.7292140/1‑94 (MQ=60)
                                                                           CGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTT                    <  SRR4302540.8818402/94‑1 (MQ=60)
                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTTGCGGGTTGATAGCCGGTTACTTG                   >  SRR4302540.7127876/1‑94 (MQ=60)
                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                   >  SRR4302540.106957/1‑94 (MQ=60)
                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                   >  SRR4302540.9675340/1‑94 (MQ=60)
                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                   >  SRR4302540.9444240/1‑94 (MQ=60)
                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                   >  SRR4302540.2327501/1‑94 (MQ=60)
                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                   >  SRR4302540.6017556/1‑94 (MQ=60)
                                                                             TTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGA                  <  SRR4302540.5204056/94‑1 (MQ=60)
                                                                              TAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAT                 <  SRR4302540.1241822/94‑1 (MQ=60)
                                                                              TAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAT                 <  SRR4302540.3330072/94‑1 (MQ=60)
                                                                              TAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAT                 <  SRR4302540.3738422/94‑1 (MQ=60)
                                                                              TAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAT                 <  SRR4302540.5473630/94‑1 (MQ=60)
                                                                               AGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGGCGGGTACTTGATG                >  SRR4302540.7657973/1‑94 (MQ=60)
                                                                               AGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATG                >  SRR4302540.619448/1‑94 (MQ=60)
                                                                               AGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATG                <  SRR4302540.3843842/94‑1 (MQ=60)
                                                                                 CGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCG              <  SRR4302540.5959730/94‑1 (MQ=60)
                                                                                 CGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCG              <  SRR4302540.6293321/94‑1 (MQ=60)
                                                                                 CGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCG              >  SRR4302540.4449093/1‑94 (MQ=60)
                                                                                  GCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGC             >  SRR4302540.9612037/1‑94 (MQ=60)
                                                                                   CCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCT            <  SRR4302540.5583332/94‑1 (MQ=60)
                                                                                   CCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCT            >  SRR4302540.3362387/1‑94 (MQ=60)
                                                                                   CCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCT            >  SRR4302540.3085262/1‑94 (MQ=60)
                                                                                    CAGGCTTTGTTGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTG           <  SRR4302540.202410/94‑1 (MQ=60)
                                                                                    CAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTG           >  SRR4302540.6260125/1‑94 (MQ=60)
                                                                                    CAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTG           <  SRR4302540.6320606/94‑1 (MQ=60)
                                                                                    CAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTG           >  SRR4302540.707193/1‑94 (MQ=60)
                                                                                    CAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTG           <  SRR4302540.3111661/94‑1 (MQ=60)
                                                                                      GGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGG         <  SRR4302540.7145453/94‑1 (MQ=60)
                                                                                      GGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGG         <  SRR4302540.1474845/94‑1 (MQ=60)
                                                                                       GCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGT        <  SRR4302540.9183955/94‑1 (MQ=60)
                                                                                       GCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGT        <  SRR4302540.6582333/94‑1 (MQ=60)
                                                                                        CTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTG       >  SRR4302540.314370/1‑94 (MQ=60)
                                                                                        CTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTG       >  SRR4302540.5196307/1‑94 (MQ=60)
                                                                                         TTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA      >  SRR4302540.2787036/1‑94 (MQ=60)
                                                                                         TTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGAGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA      >  SRR4302540.8862144/1‑94 (MQ=60)
                                                                                           TGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGATTCTCGGCGCGGACGTTGGCGGATTGATTGGCGGTTACTTGATGCGCTGGCAGAAA    >  SRR4302540.2844173/1‑94 (MQ=60)
                                                                                             TGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAAAAA  <  SRR4302540.9217368/94‑1 (MQ=60)
                                                                                             TGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAAAAA  <  SRR4302540.3937189/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAAAAA  >  NC_000913/766279‑766465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑