Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	766,372	C→T	A389V (GCG→GTG)	hrsA →	PTS system fructose specific IIABC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,372	0	C	T	100.0%	801.7 / NA	215	A389V (GCG→GTG)	hrsA	PTS system fructose specific IIABC component
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (107/108); total (107/108)

Only 100 of 219 total aligned reads displayed.

CGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA  >  NC_000913/766283‑766461

cggGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGc                                                                                        >  1:3277697/1‑93 (MQ=255)

 gtnctgctCGGAATTTTGATGGNACCGGTGCTCGCCGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCt                                                                                       <  1:4848317/90‑1 (MQ=255)

   gctgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTgg                                                                                     <  2:3049198/93‑1 (MQ=255)

     tgctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTgggc                                                                                   >  1:4009778/1‑91 (MQ=255)

      gctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACt                                                                                  <  1:4675438/93‑1 (MQ=255)

      gctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACt                                                                                  >  1:4533102/1‑93 (MQ=255)

      gctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACt                                                                                  <  1:1476941/93‑1 (MQ=255)

       ctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtt                                                                                 <  1:1210865/93‑1 (MQ=255)

       ctCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACtt                                                                                 >  2:2664406/1‑93 (MQ=255)

        tCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTg                                                                                >  2:2488869/1‑93 (MQ=255)

         cGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTgc                                                                               <  1:1806127/93‑1 (MQ=255)

           gAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTggcg                                                                             >  2:1987592/1‑93 (MQ=255)

           gAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCATATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTgccg                                                                             >  1:5174025/1‑93 (MQ=255)

               tttGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCaa                                                                         <  2:3394300/93‑1 (MQ=255)

                ttGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAAc                                                                        <  1:5549243/93‑1 (MQ=255)

                ttGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAAc                                                                        <  2:1567266/93‑1 (MQ=255)

                   aTGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATg                                                                     >  1:1239114/1‑93 (MQ=255)

                    tGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGa                                                                    <  2:4909816/93‑1 (MQ=255)

                    tGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGa                                                                    <  2:4864116/93‑1 (MQ=255)

                     ggTACCGGTGCTCGCGGCCTATACCGCCTGTTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGAt                                                                   <  1:530868/93‑1 (MQ=255)

                      gTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATc                                                                  >  1:3625572/1‑93 (MQ=255)

                      gTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATc                                                                  <  2:2112571/93‑1 (MQ=255)

                       tACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGGCTTGCCGCCAACATGATCg                                                                 >  1:193171/1‑93 (MQ=255)

                        aCCGGTGCTCGCGGCCTATACCGCCTATTTTCTGGCAGATAAACAGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCgg                                                                >  1:736067/1‑93 (MQ=255)

                        aCCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCgg                                                                <  1:5085833/93‑1 (MQ=255)

                         ccGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGc                                                               <  1:3715681/93‑1 (MQ=255)

                          cGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGGCGCCAACATGATCGGCt                                                              >  2:2761997/1‑93 (MQ=255)

                          cGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACAGGATCGGCt                                                              <  2:6125395/93‑1 (MQ=255)

                             tGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCg                                                           <  1:5243459/93‑1 (MQ=255)

                              gCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCgg                                                          >  1:533035/1‑93 (MQ=255)

                               cTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCggg                                                         <  1:1401334/93‑1 (MQ=255)

                                   cgGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtc                                                     <  1:3146807/93‑1 (MQ=255)

                                    ggCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGCTtct                                                    >  2:5587150/1‑93 (MQ=255)

                                     gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtctc                                                   <  2:1220319/93‑1 (MQ=255)

                                     gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtctc                                                   <  2:1358386/93‑1 (MQ=255)

                                     gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtctc                                                   >  2:930760/1‑93 (MQ=255)

                                     gCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTtctc                                                   <  1:2451611/93‑1 (MQ=255)

                                     gCCTATACCGCCTATTCTCTGGCAGATAAACCGGAGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCAGCTCCGGGGTtctc                                                   >  1:4867988/1‑93 (MQ=255)

                                       cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                 <  1:1590301/93‑1 (MQ=255)

                                       cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                 >  2:6170662/1‑93 (MQ=255)

                                       cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                 >  2:4749344/1‑93 (MQ=255)

                                       cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCgg                                                 <  1:5639558/93‑1 (MQ=255)

                                       cTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGACAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCgg                                                 >  2:5136757/1‑93 (MQ=255)

                                        tataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGgc                                                >  2:3493434/1‑93 (MQ=255)

                                         ataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCCACATGATCGGCTCCGGGGTTCTCTgcg                                               >  2:1446143/1‑93 (MQ=255)

                                         ataCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGgcg                                               >  2:1788909/1‑93 (MQ=255)

                                           aCCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGcgcg                                             >  1:4412443/1‑93 (MQ=255)

                                             cGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGt                                           >  2:3300628/1‑93 (MQ=255)

                                              gCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTc                                          <  1:4739003/93‑1 (MQ=255)

                                              gCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTc                                          >  2:2689810/1‑93 (MQ=255)

                                              gCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCCGCGCGGTc                                          >  1:5781557/1‑93 (MQ=255)

                                               ccTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCg                                         >  1:3788569/1‑93 (MQ=255)

                                                 tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGATTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGtactc                                       >  2:5892416/1‑89 (MQ=255)

                                                 tATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGtt                                       >  2:5629685/1‑93 (MQ=255)

                                                  aTTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTg                                      <  1:4601444/93‑1 (MQ=255)

                                                   ttCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTgg                                     >  2:5205598/1‑93 (MQ=255)

                                                      tctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGGCTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCgg                                  >  1:1964720/1‑93 (MQ=255)

                                                       ctGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGa                                 <  1:991417/93‑1 (MQ=255)

                                                        tGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGAt                                <  2:3685869/93‑1 (MQ=255)

                                                         ggCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGGCGTTGGGGGGtt                               >  1:4164991/1‑93 (MQ=255)

                                                           cAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGa                             <  2:1394591/93‑1 (MQ=255)

                                                            aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAt                            <  2:5494168/93‑1 (MQ=255)

                                                            aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAt                            >  2:5727577/1‑93 (MQ=255)

                                                            aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAt                            <  2:6279844/93‑1 (MQ=255)

                                                            aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGAt                            <  1:2716262/93‑1 (MQ=255)

                                                            aGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGAt                            >  1:3896411/1‑93 (MQ=255)

                                                             gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATa                           <  2:1134730/93‑1 (MQ=255)

                                                             gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATa                           <  1:4881229/93‑1 (MQ=255)

                                                             gATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATa                           <  1:2757954/93‑1 (MQ=255)

                                                               tAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGc                         >  2:4710276/1‑93 (MQ=255)

                                                               tAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGc                         >  2:2510006/1‑93 (MQ=255)

                                                               tAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGc                         <  1:5953813/93‑1 (MQ=255)

                                                               tAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGc                         <  1:1001415/93‑1 (MQ=255)

                                                                aaaCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGCTTGCTAGcc                        >  2:5028087/1‑93 (MQ=255)

                                                                 aaCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCg                       >  2:2211875/1‑93 (MQ=255)

                                                                 aaCCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCg                       <  2:5556803/93‑1 (MQ=255)

                                                                    cGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGtt                    >  2:4630025/1‑93 (MQ=255)

                                                                     ggCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCCACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGttc                   >  1:2845824/1‑92 (MQ=255)

                                                                     ggCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTa                   >  1:6082187/1‑93 (MQ=255)

                                                                      gCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGGTAGGCGGTTAc                  >  2:44241/1‑93 (MQ=255)

                                                                      gCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTAc                  >  1:1108745/1‑93 (MQ=255)

                                                                        gTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACtt                <  1:3643345/93‑1 (MQ=255)

                                                                         ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg               >  1:5440622/1‑93 (MQ=255)

                                                                         ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg               >  2:528288/1‑93 (MQ=255)

                                                                         ttAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGCTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTg               >  1:3886717/1‑93 (MQ=255)

                                                                          tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa              <  1:549438/93‑1 (MQ=255)

                                                                          tAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGa              >  1:4936020/1‑93 (MQ=255)

                                                                           aGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAt             <  1:6230607/93‑1 (MQ=255)

                                                                            gcgcCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATg            <  2:3882382/93‑1 (MQ=255)

                                                                             cgcCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATgc           <  2:2326913/93‑1 (MQ=255)

                                                                              gcCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGTTAGCCGGTTACTTGATgcg          >  1:3932755/1‑93 (MQ=255)

                                                                               ccAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATgcgc         <  2:959666/93‑1 (MQ=255)

                                                                                cAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCt        >  2:4119404/1‑93 (MQ=255)

                                                                                  ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTgg      <  2:515503/93‑1 (MQ=255)

                                                                                  ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTgg      <  2:3827463/93‑1 (MQ=255)

                                                                                  ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTgg      <  1:5054453/93‑1 (MQ=255)

                                                                                  ggCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTgg      <  1:3605594/93‑1 (MQ=255)

                                                                                   gCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTggg     >  1:1550004/1‑93 (MQ=255)

                                                                                     tttGTGGCTGGACTTGCCGCCAACATGATCGGCTTCGGGGTTCTCGGCGCGGTCGTTTGCGGATTGATAGCCGGGTACTTGATGAGCTGGGTg   >  2:3976165/1‑93 (MQ=255)

                                                                                     tttGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTg   >  1:2324633/1‑93 (MQ=255)

CGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA  >  NC_000913/766283‑766461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAAA  >  NC_000913/766279‑766463

GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTCTACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGT                                                                                             <  SRR4302533.6290527/94‑1 (MQ=60)

GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGT                                                                                             <  SRR4302533.3846191/94‑1 (MQ=60)

  GGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGG                                                                                           <  SRR4302533.5139047/94‑1 (MQ=60)

  GGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGG                                                                                           <  SRR4302533.2686665/94‑1 (MQ=60)

  GGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGG                                                                                           <  SRR4302533.5901098/94‑1 (MQ=60)

   GCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGC                                                                                          >  SRR4302533.3297937/1‑94 (MQ=60)

    CGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCT                                                                                         <  SRR4302533.1224185/94‑1 (MQ=60)

     GTNCTGCTCGGAATTTTGATGGNACCGGTGCTCGCCGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTG                                                                                        <  SRR4302533.4877698/94‑1 (MQ=60)

     GGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTG                                                                                        <  SRR4302533.653831/94‑1 (MQ=60)

        CTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGGC                                                                                     >  SRR4302533.4034189/1‑94 (MQ=60)

        CTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGAC                                                                                     >  SRR4302533.1881941/1‑94 (MQ=60)

         TGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACT                                                                                    <  SRR4302533.5462495/94‑1 (MQ=60)

         TGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACT                                                                                    >  SRR4302533.4560549/1‑94 (MQ=60)

          GCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTT                                                                                   <  SRR4302533.1485868/94‑1 (MQ=60)

          GCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTT                                                                                   <  SRR4302533.4703772/94‑1 (MQ=60)

           CTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTG                                                                                  <  SRR4302533.1218134/94‑1 (MQ=60)

           CTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTG                                                                                  <  SRR4302533.5360145/94‑1 (MQ=60)

           CTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTG                                                                                  <  SRR4302533.5534275/94‑1 (MQ=60)

            TCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGC                                                                                 >  SRR4302533.5053048/1‑94 (MQ=60)

             CGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCC                                                                                <  SRR4302533.1817120/94‑1 (MQ=60)

              GGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCG                                                                               <  SRR4302533.5829630/94‑1 (MQ=60)

              GGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCG                                                                               <  SRR4302533.2636248/94‑1 (MQ=60)

              GGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCATATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCG                                                                               >  SRR4302533.5205280/1‑94 (MQ=60)

                AATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCC                                                                             <  SRR4302533.3657846/94‑1 (MQ=60)

                 ATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCA                                                                            >  SRR4302533.3914564/1‑94 (MQ=60)

                  TTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAA                                                                           >  SRR4302533.2121955/1‑94 (MQ=60)

                    TTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACA                                                                         <  SRR4302533.5582754/94‑1 (MQ=60)

                      GATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATG                                                                       >  SRR4302533.1246519/1‑94 (MQ=60)

                       ATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGA                                                                      <  SRR4302533.2949933/94‑1 (MQ=60)

                        TGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACAAGAT                                                                     >  SRR4302533.4890536/1‑94 (MQ=60)

                         GGTACCGGTGCTCGCGGCCTATACCGCCTGTTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATC                                                                    <  SRR4302533.534025/94‑1 (MQ=60)

                         GGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATC                                                                    >  SRR4302533.3647878/1‑94 (MQ=60)

                          GTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGGCTTGCCGCCAACATGATCG                                                                   >  SRR4302533.194389/1‑94 (MQ=60)

                          GTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCG                                                                   <  SRR4302533.4440742/94‑1 (MQ=60)

                           TACCGGTGCTCGCGGCCTATACCGCCTATTTTCTGGCAGATAAACAGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGG                                                                  >  SRR4302533.740397/1‑94 (MQ=60)

                           TACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGG                                                                  <  SRR4302533.2771296/94‑1 (MQ=60)

                            TCCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGC                                                                 <  SRR4302533.599517/94‑1 (MQ=60)

                            ACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGC                                                                 <  SRR4302533.5116564/94‑1 (MQ=60)

                             CCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCT                                                                <  SRR4302533.3738492/94‑1 (MQ=60)

                               GGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCC                                                              <  SRR4302533.399334/94‑1 (MQ=60)

                                GTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCG                                                             <  SRR4302533.5142046/94‑1 (MQ=60)

                                 TGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGG                                                            >  SRR4302533.536205/1‑94 (MQ=60)

                                 TGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGG                                                            <  SRR4302533.5274975/94‑1 (MQ=60)

                                  GCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGG                                                           >  SRR4302533.1370828/1‑94 (MQ=60)

                                   CTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGT                                                          <  SRR4302533.3200331/94‑1 (MQ=60)

                                   CTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGT                                                          <  SRR4302533.1409784/94‑1 (MQ=60)

                                       CGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCT                                                      <  SRR4302533.3166157/94‑1 (MQ=60)

                                        GGCCTATACCGCCTATTCTCTGGCAGATAAACCGGAGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTC                                                     >  SRR4302533.4897488/1‑94 (MQ=60)

                                        GGCCTATACCGCCTATTCTCTGGCAGATAAACCGGAGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCAGCTCCGGGGTTCTC                                                     >  SRR4302533.4897487/1‑94 (MQ=60)

                                         GCCTATAGCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCG                                                    <  SRR4302533.3363459/94‑1 (MQ=60)

                                         GCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCG                                                    <  SRR4302533.1113837/94‑1 (MQ=60)

                                         GCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCG                                                    <  SRR4302533.2466745/94‑1 (MQ=60)

                                         GCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCG                                                    <  SRR4302533.5668086/94‑1 (MQ=60)

                                          CCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGG                                                   >  SRR4302533.1582798/1‑94 (MQ=60)

                                           CTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGC                                                  <  SRR4302533.1599920/94‑1 (MQ=60)

                                           CTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGC                                                  <  SRR4302533.5673649/94‑1 (MQ=60)

                                            TATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCG                                                 <  SRR4302533.3087012/94‑1 (MQ=60)

                                             ATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGC                                                >  SRR4302533.5238121/1‑94 (MQ=60)

                                              TACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCG                                               >  SRR4302533.4439087/1‑94 (MQ=60)

                                              TACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCG                                               <  SRR4302533.412717/94‑1 (MQ=60)

                                               ACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGG                                              >  SRR4302533.3784687/1‑94 (MQ=60)

                                                 CGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCCGCGCGGTC                                            >  SRR4302533.5816473/1‑94 (MQ=60)

                                                  GCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCG                                           >  SRR4302533.469204/1‑94 (MQ=60)

                                                  GCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCG                                           <  SRR4302533.4767737/94‑1 (MQ=60)

                                                  GCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCG                                           >  SRR4302533.3811771/1‑94 (MQ=60)

                                                   CCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGT                                          >  SRR4302533.1457185/1‑94 (MQ=60)

                                                    CTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTT                                         >  SRR4302533.1174592/1‑94 (MQ=60)

                                                    CTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTT                                         <  SRR4302533.6182896/94‑1 (MQ=60)

                                                     TATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTG                                        >  SRR4302533.281889/1‑94 (MQ=60)

                                                      ATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGG                                       >  SRR4302533.4628549/1‑94 (MQ=60)

                                                      ATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGG                                       <  SRR4302533.4629322/94‑1 (MQ=60)

                                                       TTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGG                                      >  SRR4302533.1945965/1‑94 (MQ=60)

                                                       TTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGC                                      <  SRR4302533.5030040/94‑1 (MQ=60)

                                                         CTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGGCTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGG                                    >  SRR4302533.1976719/1‑94 (MQ=60)

                                                          TCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGG                                   >  SRR4302533.2371449/1‑94 (MQ=60)

                                                           CTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGAT                                  <  SRR4302533.997213/94‑1 (MQ=60)

                                                            TGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATT                                 <  SRR4302533.3784295/94‑1 (MQ=60)

                                                            TGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGGCGTTGGGGGGTT                                 >  SRR4302533.4190226/1‑94 (MQ=60)

                                                               CAGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGAT                              >  SRR4302533.3920187/1‑94 (MQ=60)

                                                                AGATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATA                             <  SRR4302533.2732939/94‑1 (MQ=60)

                                                                 GATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAG                            <  SRR4302533.2774862/94‑1 (MQ=60)

                                                                 GATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAG                            <  SRR4302533.4910801/94‑1 (MQ=60)

                                                                  ATAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGC                           >  SRR4302533.4806239/1‑94 (MQ=60)

                                                                   TAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCC                          <  SRR4302533.5989827/94‑1 (MQ=60)

                                                                   TAAACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCC                          <  SRR4302533.1007262/94‑1 (MQ=60)

                                                                      ACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGT                       <  SRR4302533.4731876/94‑1 (MQ=60)

                                                                      ACCGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGT                       <  SRR4302533.3929687/94‑1 (MQ=60)

                                                                        CGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCCACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTC                     >  SRR4302533.2863338/1‑94 (MQ=60)

                                                                        CGGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTA                     >  SRR4302533.6118964/1‑94 (MQ=60)

                                                                         GGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTAC                    >  SRR4302533.3871224/1‑94 (MQ=60)

                                                                         GGCGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTAC                    >  SRR4302533.1115326/1‑94 (MQ=60)

                                                                           CGTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTT                  >  SRR4302533.4761125/1‑94 (MQ=60)

                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                 >  SRR4302533.5473387/1‑94 (MQ=60)

                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                 >  SRR4302533.1556964/1‑94 (MQ=60)

                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                 <  SRR4302533.3665746/94‑1 (MQ=60)

                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                 <  SRR4302533.5105298/94‑1 (MQ=60)

                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                 <  SRR4302533.3639710/94‑1 (MQ=60)

                                                                            GTTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGCTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTG                 >  SRR4302533.3910427/1‑94 (MQ=60)

                                                                             TTAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGA                >  SRR4302533.4965907/1‑94 (MQ=60)

                                                                              TAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAT               <  SRR4302533.552699/94‑1 (MQ=60)

                                                                              TAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAT               <  SRR4302533.1521029/94‑1 (MQ=60)

                                                                              TAGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGAT               <  SRR4302533.541798/94‑1 (MQ=60)

                                                                               AGCGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATG              <  SRR4302533.6267922/94‑1 (MQ=60)

                                                                                 CGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGTTAGCCGGTTACTTGATGCG            >  SRR4302533.3956763/1‑94 (MQ=60)

                                                                                 CGCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCG            >  SRR4302533.4332283/1‑94 (MQ=60)

                                                                                  GCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGGTACTTGATGCGC           >  SRR4302533.3675159/1‑94 (MQ=60)

                                                                                  GCCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGGTACTTGATGCGC           >  SRR4302533.5326565/1‑94 (MQ=60)

                                                                                   CCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCT          >  SRR4302533.5864601/1‑94 (MQ=60)

                                                                                   CCAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCT          >  SRR4302533.55177/1‑94 (MQ=60)

                                                                                    CAGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGGCGGTTACTTGATGCGCCG         >  SRR4302533.90528/1‑94 (MQ=60)

                                                                                     AGGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGGTACTTGATGCGCTGG        >  SRR4302533.923662/1‑94 (MQ=60)

                                                                                      GGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGG       >  SRR4302533.1559343/1‑94 (MQ=60)

                                                                                      GGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGG       <  SRR4302533.5085010/94‑1 (MQ=60)

                                                                                      GGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGG       <  SRR4302533.3627786/94‑1 (MQ=60)

                                                                                      GGCTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGG       >  SRR4302533.1697711/1‑94 (MQ=60)

                                                                                        CTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTG     >  SRR4302533.2339024/1‑94 (MQ=60)

                                                                                        CTTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTG     <  SRR4302533.5778957/94‑1 (MQ=60)

                                                                                         TTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTTGGTGA    >  SRR4302533.2284437/1‑94 (MQ=60)

                                                                                         TTTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGA    <  SRR4302533.413283/94‑1 (MQ=60)

                                                                                          TTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAA   <  SRR4302533.4942338/94‑1 (MQ=60)

                                                                                          TTGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGGTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAA   >  SRR4302533.128131/1‑94 (MQ=60)

                                                                                           TGTGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAAA  >  SRR4302533.3493562/1‑94 (MQ=60)

GCGGCGGGCTGCTCGGAATTTTGATGGTACCGGTGCTCGCGGCCTATACCGCCTATTCTCTGGCAGATAAACCGGCGTTAGCGCCAGGCTTTGCGGCTGGACTTGCCGCCAACATGATCGGCTCCGGGTTTCTCGGCGCGGTCGTTGGCGGATTGATAGCCGGTTACTTGATGCGCTGGGTGAAA  >  NC_000913/766279‑766463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`