Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	804,639	C→T	G646G (GGC→GGT)	ybhJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	804,639	0	C	T	98.9%	880.5 / ‑4.2	260	G646G (GGC→GGT)	ybhJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (140/117); total (142/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 267 total aligned reads displayed.

GTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTA  >  NC_000913/804549‑804728

gtTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGa                                                                                         <  1:2636523/93‑1 (MQ=255)

gtTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGa                                                                                         <  1:9487143/93‑1 (MQ=255)

gtTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGa                                                                                         <  2:1176259/93‑1 (MQ=255)

gtTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGa                                                                                         <  1:4038726/93‑1 (MQ=255)

 tttGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGAt                                                                                        <  1:1071134/93‑1 (MQ=255)

 tttGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGAt                                                                                        >  1:5456260/1‑93 (MQ=255)

    gcgcgcATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGt                                                                                     <  1:4677770/93‑1 (MQ=255)

     cgcgcATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGtt                                                                                    >  2:7534607/1‑93 (MQ=255)

     cgcgcATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGtt                                                                                    <  1:5433401/93‑1 (MQ=255)

     cgcgcATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGGGGTGGtt                                                                                    >  1:7624098/1‑93 (MQ=255)

       cgcATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGGGATGGtttt                                                                                  >  1:5153992/1‑91 (MQ=255)

         cATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTgc                                                                                >  1:6372101/1‑93 (MQ=255)

           ttAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTgcgc                                                                              >  1:8863438/1‑93 (MQ=255)

            tAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGggcg                                                                             >  2:7581287/1‑93 (MQ=255)

            tAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGcgcg                                                                             >  1:10010748/1‑93 (MQ=255)

            tAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGcgcg                                                                             >  1:3759040/1‑93 (MQ=255)

             aaGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGt                                                                            >  2:2155887/1‑93 (MQ=255)

               gCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGa                                                                          >  1:8584914/1‑93 (MQ=255)

               gCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGa                                                                          >  1:7855057/1‑93 (MQ=255)

                cAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGaa                                                                         <  2:2896970/93‑1 (MQ=255)

                 aGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAAc                                                                        <  2:2932366/93‑1 (MQ=255)

                  gATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACa                                                                       <  2:4276619/93‑1 (MQ=255)

                  gATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACa                                                                       <  2:8206502/93‑1 (MQ=255)

                  gATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAAATGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACa                                                                       <  2:6476616/93‑1 (MQ=255)

                     tGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAggc                                                                    >  2:2635653/1‑93 (MQ=255)

                          gTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGa                                                               <  2:10046558/93‑1 (MQ=255)

                           tCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAg                                                              >  2:2356114/1‑93 (MQ=255)

                            cAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGc                                                             <  2:4062298/93‑1 (MQ=255)

                             aGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCt                                                            >  2:5858018/1‑93 (MQ=255)

                              ggAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTg                                                           >  2:2083652/1‑93 (MQ=255)

                              ggAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTg                                                           >  1:8740730/1‑93 (MQ=255)

                                 gCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCa                                                        >  1:5585316/1‑93 (MQ=255)

                                 gCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCa                                                        >  1:9994542/1‑93 (MQ=255)

                                  cATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAg                                                       >  1:1566582/1‑93 (MQ=255)

                                  cATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAg                                                       >  2:9191197/1‑93 (MQ=255)

                                    tatTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCg                                                     >  1:2678286/1‑93 (MQ=255)

                                    tatTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCg                                                     >  2:9101380/1‑93 (MQ=255)

                                    tatTGAACCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCg                                                     >  2:7204176/1‑93 (MQ=255)

                                      ttaaTCCGCTGCAAACTGAAATTGGCGGCATGGTCTGTGCGGTGAAACCAGGTAAAGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgtg                                                   <  2:8971863/90‑1 (MQ=255)

                                          tCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAt                                               >  2:4466165/1‑93 (MQ=255)

                                          tCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAt                                               <  1:4233470/93‑1 (MQ=255)

                                           ccGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt                                              <  1:2155151/93‑1 (MQ=255)

                                              cTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGc                                           >  2:3703448/1‑93 (MQ=255)

                                               tGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg                                          >  2:1424144/1‑93 (MQ=255)

                                               tGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg                                          <  2:7920985/93‑1 (MQ=255)

                                                gCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCgg                                         <  2:7235392/93‑1 (MQ=255)

                                                gCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCgg                                         >  2:4613367/1‑93 (MQ=255)

                                                gCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCgg                                         >  2:529449/1‑93 (MQ=255)

                                                  aaaCTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTc                                       >  2:731619/1‑93 (MQ=255)

                                                   aaCTGAAATTGGCAGCATGGTCTATGCGGTGAAGCCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCt                                      <  1:5366405/93‑1 (MQ=255)

                                                    aCTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTg                                     <  1:1434465/93‑1 (MQ=255)

                                                     cTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTgg                                    >  1:5992258/1‑93 (MQ=255)

                                                      tGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGc                                   >  2:9588415/1‑93 (MQ=255)

                                                      tGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGc                                   <  2:3418944/93‑1 (MQ=255)

                                                      tGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGc                                   >  2:1532193/1‑93 (MQ=255)

                                                       gAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCg                                  >  2:6058299/1‑93 (MQ=255)

                                                         aaTTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGaa                                <  1:6906441/93‑1 (MQ=255)

                                                          aTTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCAGCCAGCGTGCGATTGGCGGTCTGGCGAat                               >  1:3574955/1‑93 (MQ=255)

                                                           ttGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAata                              <  2:5440509/93‑1 (MQ=255)

                                                           ttGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAata                              >  1:7887601/1‑93 (MQ=255)

                                                           ttGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAata                              <  2:802613/93‑1 (MQ=255)

                                                             ggCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATAtt                            <  1:2887460/93‑1 (MQ=255)

                                                              gcagcaTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTg                           >  1:7051953/1‑93 (MQ=255)

                                                               cagcaTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGc                          <  2:3244767/93‑1 (MQ=255)

                                                                agcaTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGcc                         <  2:9688860/93‑1 (MQ=255)

                                                                agcaTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGcc                         >  2:675431/1‑93 (MQ=255)

                                                                agcaTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGcc                         <  1:3261336/93‑1 (MQ=255)

                                                                agcaTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGcc                         <  1:3550956/93‑1 (MQ=255)

                                                                 gcaTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCg                        >  2:6972894/1‑93 (MQ=255)

                                                                  caTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCga                       <  1:3075352/93‑1 (MQ=255)

                                                                  caTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCga                       >  2:8718110/1‑93 (MQ=255)

                                                                  caTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCga                       <  2:5235416/93‑1 (MQ=255)

                                                                  caTGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCga                       <  1:9311496/93‑1 (MQ=255)

                                                                    tGGTCTTTGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgagg                     >  2:9880932/1‑93 (MQ=255)

                                                                    tGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgagg                     >  2:5066360/1‑93 (MQ=255)

                                                                    tGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgagg                     >  2:3166090/1‑93 (MQ=255)

                                                                     ggTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgaggg                    >  2:2737657/1‑92 (MQ=255)

                                                                     ggTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgagga                    <  1:4535433/93‑1 (MQ=255)

                                                                     ggTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgagga                    <  2:2403816/93‑1 (MQ=255)

                                                                     ggTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgagga                    <  2:8826831/93‑1 (MQ=255)

                                                                      ggcTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgaggag                   <  1:1033349/91‑1 (MQ=255)

                                                                      gTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgaggag                   >  2:6927262/1‑93 (MQ=255)

                                                                      gTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCgaggag                   >  1:8297521/1‑93 (MQ=255)

                                                                        cTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTa                 >  2:5635431/1‑93 (MQ=255)

                                                                        cTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTa                 <  1:9239573/93‑1 (MQ=255)

                                                                            gCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTAcgcg             >  1:6737315/1‑93 (MQ=255)

                                                                              ggTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGGACGCGAc           >  2:7137951/1‑93 (MQ=255)

                                                                               gTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACt          >  2:8152764/1‑93 (MQ=255)

                                                                                 gAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaa        >  1:2410380/1‑93 (MQ=255)

                                                                                 gAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaa        <  2:9625554/93‑1 (MQ=255)

                                                                                  aaaCGAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaaa       <  1:7187249/93‑1 (MQ=255)

                                                                                  aaaCCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaaa       <  1:7350471/93‑1 (MQ=255)

                                                                                  aaaCCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaaa       <  1:3407378/93‑1 (MQ=255)

                                                                                  aaaCCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaaa       <  1:5407150/93‑1 (MQ=255)

                                                                                  aaaCCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaaa       <  2:9165662/93‑1 (MQ=255)

                                                                                  aaaCCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaaa       <  1:6110859/93‑1 (MQ=255)

                                                                                  aaaCCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTaaa       >  2:185545/1‑93 (MQ=255)

                                                                                     ccAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGc    <  2:5767128/93‑1 (MQ=255)

                                                                                      cAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCt   >  2:4095778/1‑93 (MQ=255)

                                                                                       aGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTa  >  1:1987760/1‑93 (MQ=255)

GTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTA  >  NC_000913/804549‑804728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTATCGT  >  NC_000913/804547‑804732

GTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGAAAGCATGGTCTATGCGGTGAACCCAGGGG                                                                                              >  SRR4302540.5268295/1‑94 (MQ=60)

 TGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGA                                                                                             >  SRR4302540.1379280/1‑94 (MQ=60)

  GTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATCTTGAGCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGAT                                                                                            <  SRR4302540.9599622/94‑1 (MQ=60)

  GTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGAT                                                                                            <  SRR4302540.2665073/94‑1 (MQ=60)

  GTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGAT                                                                                            >  SRR4302540.5516319/1‑94 (MQ=60)

  GTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGAT                                                                                            <  SRR4302540.4082269/94‑1 (MQ=60)

  GTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGAT                                                                                            <  SRR4302540.9599620/94‑1 (MQ=60)

   TTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATG                                                                                           <  SRR4302540.1082059/94‑1 (MQ=60)

   TTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATG                                                                                           <  SRR4302540.3818270/94‑1 (MQ=60)

   TTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATG                                                                                           <  SRR4302540.8902777/94‑1 (MQ=60)

    TTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGG                                                                                          <  SRR4302540.4195223/94‑1 (MQ=60)

      GCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTT                                                                                        <  SRR4302540.4727819/94‑1 (MQ=60)

      GCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGGGGTGGTT                                                                                        >  SRR4302540.7709837/1‑94 (MQ=60)

       CGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTC                                                                                       <  SRR4302540.5493160/94‑1 (MQ=60)

        GCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGGGATGGTTTT                                                                                      >  SRR4302540.5209927/1‑94 (MQ=60)

          GCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGC                                                                                    >  SRR4302540.7611640/1‑94 (MQ=60)

          GCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGC                                                                                    >  SRR4302540.6443051/1‑94 (MQ=60)

            ATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGC                                                                                  >  SRR4302540.8966004/1‑94 (MQ=60)

            ATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGCTCTATGCGGTGAAACCAGGTGATGGTTCTGCGG                                                                                  >  SRR4302540.3322835/1‑94 (MQ=60)

             TTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCG                                                                                 >  SRR4302540.7973824/1‑94 (MQ=60)

             TTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCG                                                                                 >  SRR4302540.10127313/1‑94 (MQ=60)

             TTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCG                                                                                 >  SRR4302540.3800048/1‑94 (MQ=60)

                AGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGA                                                                              >  SRR4302540.7946841/1‑94 (MQ=60)

                AGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGA                                                                              >  SRR4302540.8684718/1‑94 (MQ=60)

                  CAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAAC                                                                            <  SRR4302540.5447977/94‑1 (MQ=60)

                  CAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAAC                                                                            >  SRR4302540.2698213/1‑94 (MQ=60)

                   AGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCTCGTGAACA                                                                           >  SRR4302540.7144410/1‑94 (MQ=60)

                   AGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACA                                                                           >  SRR4302540.628941/1‑94 (MQ=60)

                    GATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAG                                                                          >  SRR4302540.7719624/1‑94 (MQ=60)

                    GATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAG                                                                          >  SRR4302540.5429366/1‑94 (MQ=60)

                         CTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGC                                                                     <  SRR4302540.8317171/94‑1 (MQ=60)

                         CTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGC                                                                     <  SRR4302540.316931/94‑1 (MQ=60)

                         CTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGC                                                                     >  SRR4302540.7055321/1‑94 (MQ=60)

                           GGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGA                                                                   >  SRR4302540.7853263/1‑94 (MQ=60)

                           GGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGA                                                                   >  SRR4302540.3512776/1‑94 (MQ=60)

                            GTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAG                                                                  >  SRR4302540.1556256/1‑94 (MQ=60)

                            GTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGNNNNGCGCGTGAACNGNCGGgngn                                                                  >  SRR4302540.3024324/1‑90 (MQ=60)

                              CAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCT                                                                >  SRR4302540.4071208/1‑94 (MQ=60)

                              CAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCT                                                                >  SRR4302540.1836692/1‑94 (MQ=60)

                              CAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCT                                                                <  SRR4302540.9416613/94‑1 (MQ=60)

                               AGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTG                                                               >  SRR4302540.8842197/1‑94 (MQ=60)

                               AGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTG                                                               >  SRR4302540.4682128/1‑94 (MQ=60)

                               AGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTG                                                               >  SRR4302540.7581194/1‑94 (MQ=60)

                                 GAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCC                                                             <  SRR4302540.9940871/94‑1 (MQ=60)

                                  AGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCA                                                            >  SRR4302540.10111012/1‑94 (MQ=60)

                                  AGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCA                                                            >  SRR4302540.5646785/1‑94 (MQ=60)

                                  AGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCA                                                            >  SRR4302540.5646786/1‑94 (MQ=60)

                                   GCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAG                                                           >  SRR4302540.1583767/1‑94 (MQ=60)

                                    CATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGC                                                          >  SRR4302540.5944612/1‑94 (MQ=60)

                                     ATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCG                                                         >  SRR4302540.2707268/1‑94 (MQ=60)

                                     ATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCG                                                         >  SRR4302540.6001344/1‑94 (MQ=60)

                                     ATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCG                                                         >  SRR4302540.9511244/1‑94 (MQ=60)

                                     ATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCG                                                         <  SRR4302540.4636865/94‑1 (MQ=60)

                                     ATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCG                                                         >  SRR4302540.4248079/1‑94 (MQ=60)

                                        TTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGT                                                      >  SRR4302540.2868847/1‑94 (MQ=60)

                                         TGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTG                                                     >  SRR4302540.1046304/1‑94 (MQ=60)

                                         TGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTG                                                     >  SRR4302540.5476678/1‑94 (MQ=60)

                                         TGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTG                                                     >  SRR4302540.266188/1‑94 (MQ=60)

                                          GATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGA                                                    <  SRR4302540.2323825/94‑1 (MQ=60)

                                           ATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCTCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAT                                                   <  SRR4302540.5484423/94‑1 (MQ=60)

                                           ATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAT                                                   >  SRR4302540.8412830/1‑94 (MQ=60)

                                           ATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAT                                                   >  SRR4302540.8412831/1‑94 (MQ=60)

                                            TCCGCTGCAAACTGAAATTGGCAGCATGGTGTATGCGATGAATCACGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATT                                                  <  SRR4302540.8669911/94‑1 (MQ=60)

                                            TCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATT                                                  <  SRR4302540.4279541/94‑1 (MQ=60)

                                            TCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATT                                                  >  SRR4302540.637947/1‑94 (MQ=60)

                                             CCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTG                                                 <  SRR4302540.2178344/94‑1 (MQ=60)

                                             CCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTG                                                 >  SRR4302540.8858950/1‑94 (MQ=60)

                                             CCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTG                                                 >  SRR4302540.3609606/1‑94 (MQ=60)

                                              CGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGCTGGTTCTGCGCGTGAACAGGCGGtgagcctcctgcgtgagattgc                                                >  SRR4302540.268664/1‑72 (MQ=60)

                                              CGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGGTTGG                                                >  SRR4302540.7278621/1‑94 (MQ=60)

                                               GCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGC                                               >  SRR4302540.7090453/1‑94 (MQ=60)

                                                 TGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGG                                             <  SRR4302540.128696/94‑1 (MQ=60)

                                                     AACTGAAATTGGCAGCATGGTCTATGCGGTGAAGCCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTG                                         <  SRR4302540.5425367/94‑1 (MQ=60)

                                                     AACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTG                                         >  SRR4302540.5494088/1‑94 (MQ=60)

                                                      ACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGG                                        <  SRR4302540.8738167/94‑1 (MQ=60)

                                                      ACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGG                                        <  SRR4302540.1450016/94‑1 (MQ=60)

                                                      ACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGG                                        >  SRR4302540.6058644/1‑94 (MQ=60)

                                                       CTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGC                                       >  SRR4302540.2178293/1‑94 (MQ=60)

                                                        NNNAATTNGCAGCATGGNCTANGNGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCG                                      <  SRR4302540.7885931/94‑1 (MQ=60)

                                                         GAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGA                                     >  SRR4302540.168140/1‑94 (MQ=60)

                                                           AATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCAGCCAGCGTGCGATTGGCGGTCTGGCGAAT                                   >  SRR4302540.3614002/1‑94 (MQ=60)

                                                           AATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAAT                                   <  SRR4302540.6983239/94‑1 (MQ=60)

                                                            ATTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATA                                  >  SRR4302540.7979670/1‑94 (MQ=60)

                                                             TTGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATAT                                 <  SRR4302540.6814754/94‑1 (MQ=60)

                                                              TGGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATT                                >  SRR4302540.9145407/1‑94 (MQ=60)

                                                               GGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGATCAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTG                               >  SRR4302540.297337/1‑94 (MQ=60)

                                                               GGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTG                               >  SRR4302540.7130379/1‑94 (MQ=60)

                                                               GGCAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTG                               <  SRR4302540.2918902/94‑1 (MQ=60)

                                                                 CAGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCC                             <  SRR4302540.483301/94‑1 (MQ=60)

                                                                  AGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCG                            <  SRR4302540.3589754/94‑1 (MQ=60)

                                                                  AGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCG                            <  SRR4302540.3589753/94‑1 (MQ=60)

                                                                  AGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCG                            <  SRR4302540.3296918/94‑1 (MQ=60)

                                                                  AGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCG                            >  SRR4302540.3180032/1‑94 (MQ=60)

                                                                  AGCATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCG                            >  SRR4302540.5605438/1‑94 (MQ=60)

                                                                    CATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAG                          <  SRR4302540.3109091/94‑1 (MQ=60)

                                                                    CATGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAG                          <  SRR4302540.9422649/94‑1 (MQ=60)

                                                                      TGGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGA                        <  SRR4302540.9488095/94‑1 (MQ=60)

                                                                       GGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAG                       <  SRR4302540.4584527/94‑1 (MQ=60)

                                                                       GGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAG                       >  SRR4302540.8393866/1‑94 (MQ=60)

                                                                       GGTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAG                       <  SRR4302540.1042832/94‑1 (MQ=60)

                                                                        GTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGT                      <  SRR4302540.1043859/94‑1 (MQ=60)

                                                                        GTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGT                      <  SRR4302540.300133/94‑1 (MQ=60)

                                                                        GTCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGT                      >  SRR4302540.2639894/1‑94 (MQ=60)

                                                                        GGCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGT                      <  SRR4302540.1043858/94‑1 (MQ=60)

                                                                         TCTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTA                     <  SRR4302540.3666889/94‑1 (MQ=60)

                                                                          CTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTAC                    >  SRR4302540.8173352/1‑94 (MQ=60)

                                                                          CTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTAC                    <  SRR4302540.9156758/94‑1 (MQ=60)

                                                                          CTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTAC                    <  SRR4302540.6158257/94‑1 (MQ=60)

                                                                          CTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTAC                    <  SRR4302540.9350252/94‑1 (MQ=60)

                                                                          CTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTAC                    <  SRR4302540.4302775/94‑1 (MQ=60)

                                                                          CTATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGGAC                    >  SRR4302540.2079668/1‑94 (MQ=60)

                                                                           TATGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACG                   >  SRR4302540.3551297/1‑94 (MQ=60)

                                                                             TGCGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCG                 >  SRR4302540.6811733/1‑94 (MQ=60)

                                                                               CGGTGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGAC               >  SRR4302540.4946949/1‑94 (MQ=60)

                                                                                  TGAAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAA            >  SRR4302540.2436367/1‑94 (MQ=60)

                                                                                    AAACGAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAAC          <  SRR4302540.7267364/94‑1 (MQ=60)

                                                                                    AAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAAC          <  SRR4302540.3444499/94‑1 (MQ=60)

                                                                                    AAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAAC          <  SRR4302540.5466611/94‑1 (MQ=60)

                                                                                    AAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAAC          <  SRR4302540.6178903/94‑1 (MQ=60)

                                                                                    AAACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAAC          <  SRR4302540.7432467/94‑1 (MQ=60)

                                                                                     AACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACG         <  SRR4302540.7255597/94‑1 (MQ=60)

                                                                                      ACCAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGC        <  SRR4302540.5422552/94‑1 (MQ=60)

                                                                                        CAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTA      >  SRR4302540.2009305/1‑94 (MQ=60)

                                                                                        CAGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTA      >  SRR4302540.6580766/1‑94 (MQ=60)

                                                                                         AGGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTAT     <  SRR4302540.6499334/94‑1 (MQ=60)

                                                                                          GGTGATGGTTCTGCGCGTGAGCAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTATC    <  SRR4302540.7138834/94‑1 (MQ=60)

                                                                                          GGTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTATC    >  SRR4302540.7818836/1‑94 (MQ=60)

                                                                                          GGTGAGGGTTCTGCCAGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGAGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTATC    <  SRR4302540.3270188/94‑1 (MQ=60)

                                                                                           GTGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTATCG   >  SRR4302540.9781003/1‑94 (MQ=60)

                                                                                            TGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGTGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGGTATCGT  >  SRR4302540.2716467/1‑94 (MQ=60)

                                                                                            TGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTATCGT  <  SRR4302540.7670630/94‑1 (MQ=60)

                                                                                            TGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTATCGT  >  SRR4302540.5221754/1‑94 (MQ=60)

GTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTGGCGAATATTGCCGAGGAGTACGCGACTAAACGCTATCGT  >  NC_000913/804547‑804732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`