Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	827,018	C→T	S396N (AGT→AAT)	ybhF ←	YbhF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	827,018	0	C	T	99.6%	915.2 / ‑5.5	253	S396N (AGT→AAT)	ybhF	YbhF
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (129/123); total (129/124)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 275 total aligned reads displayed.

AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCC  >  NC_000913/826928‑827107

aaaaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttc                                                                                         >  2:7594610/1‑93 (MQ=255)

aaaaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttc                                                                                         <  1:4631481/93‑1 (MQ=255)

aaaaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttc                                                                                         <  2:2418595/93‑1 (MQ=255)

 aaaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttct                                                                                        >  1:2784959/1‑93 (MQ=255)

  aaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCGTTACCGGAAttctc                                                                                       >  2:1223112/1‑93 (MQ=255)

   aaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttctct                                                                                      >  2:6492254/1‑93 (MQ=255)

    aGCGTAAATTCTGTCCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCAATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCtt                                                                                     <  1:243671/93‑1 (MQ=255)

     gCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCttt                                                                                    <  1:6254503/93‑1 (MQ=255)

       gTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCa                                                                                  >  1:66260/1‑93 (MQ=255)

       gTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCa                                                                                  <  1:9147909/93‑1 (MQ=255)

       gTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCa                                                                                  <  1:9642046/93‑1 (MQ=255)

       gTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCa                                                                                  <  1:9906805/93‑1 (MQ=255)

        tAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAg                                                                                 >  1:1284168/1‑93 (MQ=255)

        tAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAg                                                                                 <  1:7535217/93‑1 (MQ=255)

         aaaTTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGa                                                                                <  2:9475544/93‑1 (MQ=255)

          aaTTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGat                                                                               <  2:3208338/93‑1 (MQ=255)

           aTTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatc                                                                              >  1:3034103/1‑93 (MQ=255)

              cTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatccat                                                                           <  1:3709500/93‑1 (MQ=255)

              cTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatccat                                                                           <  2:3458709/93‑1 (MQ=255)

              cTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatccat                                                                           >  1:8160017/1‑93 (MQ=255)

              cTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatccat                                                                           >  2:5980013/1‑93 (MQ=255)

               tGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatccatc                                                                          <  2:1275244/93‑1 (MQ=255)

               tGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGatccatc                                                                          <  1:4894556/93‑1 (MQ=255)

                 ttCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATccc                                                                        >  2:3864482/1‑93 (MQ=255)

                   cGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTTTTTCAGATCCATCCCCa                                                                      <  2:2552283/93‑1 (MQ=255)

                   cGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCa                                                                      <  2:1592899/93‑1 (MQ=255)

                     aCCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGc                                                                    <  1:7189638/93‑1 (MQ=255)

                        gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCAcc                                                                 >  1:9445166/1‑93 (MQ=255)

                         tCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCa                                                                >  1:9386257/1‑93 (MQ=255)

                         tCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCa                                                                >  1:1040519/1‑93 (MQ=255)

                         tCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCa                                                                >  1:1896678/1‑93 (MQ=255)

                            ggTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgcg                                                             >  2:4325138/1‑93 (MQ=255)

                             gTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTGAGATCCATCCCCAGCACCAgcgc                                                            >  2:7208726/1‑93 (MQ=255)

                             gTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgcgc                                                            >  1:5774306/1‑93 (MQ=255)

                             gTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgcgc                                                            >  1:1690748/1‑93 (MQ=255)

                              ttACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGcc                                                           <  2:4651613/93‑1 (MQ=255)

                               tACCGTAGAACGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCt                                                          >  2:8261872/1‑93 (MQ=255)

                                 ccGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTgg                                                        >  1:4432471/1‑93 (MQ=255)

                                 ccGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTgg                                                        >  2:3932444/1‑93 (MQ=255)

                                  cGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGc                                                       >  2:892043/1‑93 (MQ=255)

                                  cGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGc                                                       <  2:5242159/93‑1 (MQ=255)

                                   gTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGcc                                                      <  2:1457547/93‑1 (MQ=255)

                                    tAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCg                                                     <  2:9262195/93‑1 (MQ=255)

                                    tAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCg                                                     <  1:4115307/93‑1 (MQ=255)

                                    tAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCg                                                     <  1:3692600/93‑1 (MQ=255)

                                    tAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCg                                                     >  2:6942605/1‑93 (MQ=255)

                                    tAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCg                                                     >  1:6448443/1‑93 (MQ=255)

                                    tAGAGCGAAAATTTTGGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCg                                                     <  1:2196849/93‑1 (MQ=255)

                                     agagCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCgg                                                    >  1:563132/1‑93 (MQ=255)

                                      gagCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGa                                                   >  2:5629452/1‑93 (MQ=255)

                                         cGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGt                                                <  1:1997116/93‑1 (MQ=255)

                                         cGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGt                                                <  1:3836099/93‑1 (MQ=255)

                                          gAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTc                                               <  2:2936821/93‑1 (MQ=255)

                                           aaaaTTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCg                                              <  2:1118410/93‑1 (MQ=255)

                                           aaaaTTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCg                                              <  1:6836275/93‑1 (MQ=255)

                                            aaaTTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCgg                                             >  2:3138196/1‑93 (MQ=255)

                                             aaTTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGc                                            <  1:7386019/93‑1 (MQ=255)

                                                ttttGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCAcc                                         <  2:5764557/93‑1 (MQ=255)

                                                 tttGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCa                                        <  2:4821777/93‑1 (MQ=255)

                                                 tttGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCa                                        <  2:1876427/93‑1 (MQ=255)

                                                    gcgcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCa                                     >  2:5128550/1‑93 (MQ=255)

                                                     cgcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCaa                                    <  2:6368259/93‑1 (MQ=255)

                                                      gcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCaaa                                   <  1:3502548/93‑1 (MQ=255)

                                                       ccATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAAc                                  <  1:6333475/93‑1 (MQ=255)

                                                       ccATATAGCCGAGATGCAGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAAc                                  <  1:8980488/93‑1 (MQ=255)

                                                         atatAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCg                                <  1:10035767/93‑1 (MQ=255)

                                                         atatAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCg                                >  1:8652814/1‑93 (MQ=255)

                                                         atatAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCg                                <  1:4489228/93‑1 (MQ=255)

                                                          tataGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGGTCCATCCCCAGCACCAGCGGCTGGCCGGAAGTCGGCACCAGCAAACCGc                               >  1:2314304/1‑93 (MQ=255)

                                                          tataGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCga                               >  2:8191016/1‑92 (MQ=255)

                                                          tataGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGc                               <  1:2881725/93‑1 (MQ=255)

                                                          tataGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGc                               >  1:4913437/1‑93 (MQ=255)

                                                            taGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGcac                             >  2:6154809/1‑93 (MQ=255)

                                                             aGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGcaca                            <  1:7506751/93‑1 (MQ=255)

                                                             aGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGcaca                            >  1:8286072/1‑93 (MQ=255)

                                                               ccGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatc                          >  1:7955214/1‑93 (MQ=255)

                                                                cGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatcc                         >  1:1823868/1‑92 (MQ=255)

                                                                cGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatca                         >  2:8591574/1‑93 (MQ=255)

                                                                 gagtTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatcat                        >  2:7687701/1‑93 (MQ=255)

                                                                 gagaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatcat                        >  1:8834513/1‑93 (MQ=255)

                                                                 gagaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatcat                        >  1:2247896/1‑93 (MQ=255)

                                                                 gagaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatcat                        >  1:3405691/1‑93 (MQ=255)

                                                                 gagaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatcat                        >  2:7369075/1‑93 (MQ=255)

                                                                  agaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACatcatc                       >  2:405456/1‑93 (MQ=255)

                                                                   gaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCTCATCATCt                      >  2:4611992/1‑93 (MQ=255)

                                                                    aTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCtt                     <  2:8483403/93‑1 (MQ=255)

                                                                      gCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTaa                   <  2:2756677/93‑1 (MQ=255)

                                                                      gCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTaa                   <  2:805456/93‑1 (MQ=255)

                                                                       cTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTaaa                  <  1:5347567/93‑1 (MQ=255)

                                                                        tGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAg                 <  2:5340179/93‑1 (MQ=255)

                                                                         ggCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAgg                <  2:9509215/93‑1 (MQ=255)

                                                                          gcgcgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggt               <  1:8459176/93‑1 (MQ=255)

                                                                          gcgcgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggt               <  2:9307071/93‑1 (MQ=255)

                                                                             cgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggtggt            >  1:4951021/1‑93 (MQ=255)

                                                                             cgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggtggt            >  2:1739218/1‑93 (MQ=255)

                                                                                  tACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGAtt       <  2:776730/93‑1 (MQ=255)

                                                                                   aCCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGAttt      >  1:7746249/1‑93 (MQ=255)

                                                                                   aCCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGAttt      >  1:9753620/1‑93 (MQ=255)

                                                                                     cGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTAc    >  1:6029485/1‑93 (MQ=255)

                                                                                      ggAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTAcc   <  2:3106407/93‑1 (MQ=255)

AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCC  >  NC_000913/826928‑827107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGC  >  NC_000913/826925‑827111

GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAT                                                                                               <  SRR4302540.9638549/94‑1 (MQ=60)

GAGAAAAAGCGTAAATTCTGTTCGACCGTCACGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGAGCGCTTTACCGGAAT                                                                                               >  SRR4302540.5221931/1‑94 (MQ=60)

 AGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATT                                                                                              <  SRR4302540.9708618/94‑1 (MQ=60)

 AGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATT                                                                                              >  SRR4302540.8170355/1‑94 (MQ=60)

  GAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTC                                                                                             <  SRR4302540.2914384/94‑1 (MQ=60)

  GAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTC                                                                                             <  SRR4302540.6050212/94‑1 (MQ=60)

   AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCT                                                                                            >  SRR4302540.2815042/1‑94 (MQ=60)

   AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCT                                                                                            <  SRR4302540.6396661/94‑1 (MQ=60)

   AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCT                                                                                            <  SRR4302540.4681086/94‑1 (MQ=60)

   AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCT                                                                                            <  SRR4302540.7799955/94‑1 (MQ=60)

   AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTATACCGGAATTCT                                                                                            <  SRR4302540.9996158/94‑1 (MQ=60)

    AAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTC                                                                                           >  SRR4302540.6708751/1‑94 (MQ=60)

     AAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCT                                                                                          <  SRR4302540.4566351/94‑1 (MQ=60)

     AAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCT                                                                                          <  SRR4302540.1694414/94‑1 (MQ=60)

      AAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTT                                                                                         <  SRR4302540.5804574/94‑1 (MQ=60)

      AAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTT                                                                                         <  SRR4302540.1928530/94‑1 (MQ=60)

       AGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTT                                                                                        >  SRR4302540.9516691/1‑94 (MQ=60)

       AGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTT                                                                                        <  SRR4302540.8118991/94‑1 (MQ=60)

       AGCGTAAATTCTGTCCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCAATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTT                                                                                        <  SRR4302540.246021/94‑1 (MQ=60)

        GCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTC                                                                                       <  SRR4302540.6324092/94‑1 (MQ=60)

         CGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCA                                                                                      >  SRR4302540.3318985/1‑94 (MQ=60)

         CGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCA                                                                                      <  SRR4302540.8516958/94‑1 (MQ=60)

         CGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCA                                                                                      >  SRR4302540.4721404/1‑94 (MQ=60)

         CGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCA                                                                                      >  SRR4302540.66932/1‑94 (MQ=60)

          GTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAG                                                                                     <  SRR4302540.9257770/94‑1 (MQ=60)

          GTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAG                                                                                     <  SRR4302540.9755622/94‑1 (MQ=60)

          GTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAG                                                                                     >  SRR4302540.1297404/1‑94 (MQ=60)

          GTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAG                                                                                     <  SRR4302540.10022530/94‑1 (MQ=60)

           TAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGA                                                                                    >  SRR4302540.9226044/1‑94 (MQ=60)

           TAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGA                                                                                    <  SRR4302540.7619610/94‑1 (MQ=60)

            AAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGAT                                                                                   <  SRR4302540.6105167/94‑1 (MQ=60)

             AATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATC                                                                                  <  SRR4302540.3162038/94‑1 (MQ=60)

             AATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATC                                                                                  >  SRR4302540.3067402/1‑94 (MQ=60)

               TTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCA                                                                                >  SRR4302540.7233945/1‑94 (MQ=60)

                tgtgtgcgccgGTCAGGGTACCGTAGAGCTAAAGTGTTTGCGGCATATACCCGAGAGGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               <  SRR4302540.471317/83‑1 (MQ=44)

                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               <  SRR4302540.7610690/94‑1 (MQ=60)

                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               >  SRR4302540.8254768/1‑94 (MQ=60)

                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               >  SRR4302540.5060310/1‑94 (MQ=60)

                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               <  SRR4302540.4420778/94‑1 (MQ=60)

                 CTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATC                                                                              <  SRR4302540.7871730/94‑1 (MQ=60)

                 CTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATC                                                                              <  SRR4302540.1911935/94‑1 (MQ=60)

                 CTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATC                                                                              <  SRR4302540.3749986/94‑1 (MQ=60)

                  TGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCC                                                                             <  SRR4302540.7613359/94‑1 (MQ=60)

                  TGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCC                                                                             <  SRR4302540.4947193/94‑1 (MQ=60)

                   GTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCC                                                                            <  SRR4302540.8484958/94‑1 (MQ=60)

                    TTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCC                                                                           <  SRR4302540.2786432/94‑1 (MQ=60)

                      CGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAG                                                                         >  SRR4302540.9485536/1‑94 (MQ=60)

                       GACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGC                                                                        >  SRR4302540.4794798/1‑94 (MQ=60)

                        ACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCA                                                                       <  SRR4302540.7269777/94‑1 (MQ=60)

                          CGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACC                                                                     >  SRR4302540.2953106/1‑94 (MQ=60)

                          CGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACC                                                                     >  SRR4302540.9557297/1‑94 (MQ=60)

                          CGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACC                                                                     >  SRR4302540.4978265/1‑94 (MQ=60)

                           GTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCA                                                                    >  SRR4302540.919503/1‑94 (MQ=60)

                           GTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCA                                                                    >  SRR4302540.1917430/1‑94 (MQ=60)

                           GTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCA                                                                    >  SRR4302540.1051099/1‑94 (MQ=60)

                           GTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCA                                                                    >  SRR4302540.9497976/1‑94 (MQ=60)

                            TCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAG                                                                   <  SRR4302540.1102968/94‑1 (MQ=60)

                             CAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGC                                                                  >  SRR4302540.3958948/1‑94 (MQ=60)

                             CAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCACCAGCACCAGC                                                                  >  SRR4302540.5540690/1‑94 (MQ=60)

                              AGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCG                                                                 <  SRR4302540.2003427/94‑1 (MQ=60)

                               GGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGC                                                                >  SRR4302540.5838089/1‑94 (MQ=60)

                               GGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGC                                                                >  SRR4302540.1709082/1‑94 (MQ=60)

                               GGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGC                                                                <  SRR4302540.1592890/94‑1 (MQ=60)

                                GTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCC                                                               <  SRR4302540.3723728/94‑1 (MQ=60)

                                   ACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGG                                                            >  SRR4302540.4480492/1‑94 (MQ=60)

                                     CGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCC                                                          >  SRR4302540.2864328/1‑94 (MQ=60)

                                     CGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCC                                                          >  SRR4302540.9835576/1‑94 (MQ=60)

                                     CGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCC                                                          >  SRR4302540.1776486/1‑94 (MQ=60)

                                     CGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCC                                                          >  SRR4302540.3712694/1‑94 (MQ=60)

                                      GTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCG                                                         >  SRR4302540.6520224/1‑94 (MQ=60)

                                       TAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGG                                                        >  SRR4302540.3523436/1‑94 (MQ=60)

                                       TAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGG                                                        <  SRR4302540.3732931/94‑1 (MQ=60)

                                       TAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGG                                                        >  SRR4302540.569256/1‑94 (MQ=60)

                                       TAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGG                                                        <  SRR4302540.4159735/94‑1 (MQ=60)

                                       TAGAGCGAAAATTTTGGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGG                                                        <  SRR4302540.2220574/94‑1 (MQ=60)

                                          AGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAG                                                     <  SRR4302540.449052/94‑1 (MQ=60)

                                            CGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTC                                                   <  SRR4302540.3877829/94‑1 (MQ=60)

                                            CGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTC                                                   <  SRR4302540.2018752/94‑1 (MQ=60)

                                             GAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCG                                                  >  SRR4302540.5821073/1‑94 (MQ=60)

                                             GAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCG                                                  >  SRR4302540.6178992/1‑94 (MQ=60)

                                              AAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGG                                                 <  SRR4302540.6911994/94‑1 (MQ=60)

                                              AAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGG                                                 <  SRR4302540.8271775/94‑1 (MQ=60)

                                                AATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCA                                               <  SRR4302540.7468312/94‑1 (MQ=60)

                                                 ATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCAC                                              <  SRR4302540.9374887/94‑1 (MQ=60)

                                                 ATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCAC                                              >  SRR4302540.6048703/1‑94 (MQ=60)

                                                  TTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACC                                             >  SRR4302540.8772876/1‑94 (MQ=60)

                                                   TTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCA                                            <  SRR4302540.6696594/94‑1 (MQ=60)

                                                   TTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCA                                            >  SRR4302540.9678549/1‑94 (MQ=60)

                                                       TCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTACAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAA                                        <  SRR4302540.2260964/94‑1 (MQ=60)

                                                         GCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAAC                                      <  SRR4302540.3540694/94‑1 (MQ=60)

                                                          CCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACC                                     <  SRR4302540.896704/94‑1 (MQ=60)

                                                          CCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACC                                     <  SRR4302540.6404001/94‑1 (MQ=60)

                                                          CCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACC                                     <  SRR4302540.6404000/94‑1 (MQ=60)

                                                          CCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAACTCGGCACCAGCAAACC                                     <  SRR4302540.7513688/94‑1 (MQ=60)

                                                          CCATATAGCCGAGATGCAGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACC                                     <  SRR4302540.9087508/94‑1 (MQ=60)

                                                           CATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCG                                    <  SRR4302540.3982832/94‑1 (MQ=60)

                                                           CATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCG                                    <  SRR4302540.2626233/94‑1 (MQ=60)

                                                           CATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCG                                    >  SRR4302540.8753329/1‑94 (MQ=60)

                                                           CATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCG                                    >  SRR4302540.4665419/1‑94 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTCCCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   <  SRR4302540.10152485/94‑1 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGGTCCATCCCCAGCACCAGCGGCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   >  SRR4302540.2339218/1‑94 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   >  SRR4302540.9755300/1‑94 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   >  SRR4302540.8662909/1‑94 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   <  SRR4302540.10152484/94‑1 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   >  SRR4302540.4966345/1‑94 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   <  SRR4302540.4537911/94‑1 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTCGCCGGCAGCCGGCACCAGCGAACCTC                                   >  SRR4302540.4966343/1‑94 (MQ=60)

                                                            ATATAGCCGAGATGCTGGCGCGCTTTACAGGAATTCTCTTTCAGATCCATCCCCAGAACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   >  SRR4302540.8662902/1‑94 (MQ=60)

                                                             TATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCTCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCA                                  >  SRR4302540.7730216/1‑94 (MQ=60)

                                                             TATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCA                                  <  SRR4302540.2913080/94‑1 (MQ=60)

                                                               TAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACA                                >  SRR4302540.8382311/1‑94 (MQ=60)

                                                                AGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACAT                               >  SRR4302540.3815901/1‑94 (MQ=60)

                                                                AGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACAT                               <  SRR4302540.7590656/94‑1 (MQ=60)

                                                                 GCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATC                              >  SRR4302540.8047948/1‑94 (MQ=60)

                                                                 GCCGAGATGCTGGCGCGCTTTACCGGAATTATCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATC                              <  SRR4302540.7171662/94‑1 (MQ=60)

                                                                  GCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCA                             <  SRR4302540.4964187/94‑1 (MQ=60)

                                                                  CCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCC                             >  SRR4302540.1843805/1‑94 (MQ=60)

                                                                   CGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCAT                            >  SRR4302540.3442804/1‑94 (MQ=60)

                                                                   CGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCAT                            >  SRR4302540.8936692/1‑94 (MQ=60)

                                                                   CGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCAT                            >  SRR4302540.2272041/1‑94 (MQ=60)

                                                                    GAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATC                           >  SRR4302540.4329674/1‑94 (MQ=60)

                                                                     AGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCT                          >  SRR4302540.9863460/1‑94 (MQ=60)

                                                                      GATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTT                         >  SRR4302540.646724/1‑94 (MQ=60)

                                                                       ATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTA                        <  SRR4302540.1203098/94‑1 (MQ=60)

                                                                       ATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTA                        >  SRR4302540.5117107/1‑94 (MQ=60)

                                                                         GCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAA                      <  SRR4302540.2887429/94‑1 (MQ=60)

                                                                          CTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAG                     <  SRR4302540.5406244/94‑1 (MQ=60)

                                                                          CTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAG                     <  SRR4302540.3741881/94‑1 (MQ=60)

                                                                           TGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGG                    <  SRR4302540.2370031/94‑1 (MQ=60)

                                                                           GGGCGCGCTTTACCGGAATGCTCTTTCAGATCCAGCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGG                    <  SRR4302540.2370032/94‑1 (MQ=60)

                                                                             GCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTG                  <  SRR4302540.8557393/94‑1 (MQ=60)

                                                                              CGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGG                 <  SRR4302540.8273607/94‑1 (MQ=60)

                                                                              CGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGG                 <  SRR4302540.1760816/94‑1 (MQ=60)

                                                                               GCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGT                >  SRR4302540.5004508/1‑94 (MQ=60)

                                                                                CGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTC               <  SRR4302540.7375205/94‑1 (MQ=60)

                                                                                 GCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCG              >  SRR4302540.7975082/1‑94 (MQ=60)

                                                                                 GCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGGGGTCG              >  SRR4302540.206002/1‑94 (MQ=60)

                                                                                  CTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGA             <  SRR4302540.8969366/94‑1 (MQ=60)

                                                                                   TTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGAT            <  SRR4302540.7323701/94‑1 (MQ=60)

                                                                                   TTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGAT            >  SRR4302540.5505332/1‑94 (MQ=60)

                                                                                    TTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATT           <  SRR4302540.2273080/94‑1 (MQ=60)

                                                                                     TACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTT          >  SRR4302540.9868057/1‑94 (MQ=60)

                                                                                     TACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTT          >  SRR4302540.7836695/1‑94 (MQ=60)

                                                                                     TACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTT          <  SRR4302540.6799851/94‑1 (MQ=60)

                                                                                      ACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTA         <  SRR4302540.22648/94‑1 (MQ=60)

                                                                                       CCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTAC        <  SRR4302540.648769/94‑1 (MQ=60)

                                                                                       CCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTAC        >  SRR4302540.6096393/1‑94 (MQ=60)

                                                                                        CGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACC       >  SRR4302540.2138641/1‑94 (MQ=60)

                                                                                        CGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACC       >  SRR4302540.3763639/1‑94 (MQ=60)

                                                                                         GGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCC      >  SRR4302540.9934922/1‑94 (MQ=60)

                                                                                           AATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGC    >  SRR4302540.6059386/1‑94 (MQ=60)

                                                                                           AATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGC    <  SRR4302540.551227/94‑1 (MQ=60)

                                                                                            ATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCG   >  SRR4302540.440131/1‑94 (MQ=60)

                                                                                             TTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGC  >  SRR4302540.2157286/1‑94 (MQ=60)

GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGC  >  NC_000913/826925‑827111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`