Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 827,018 C→T S396N (AGT→AAT)  ybhF ← YbhF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913827,0180CT100.0% 647.8 / NA 177S396N (AGT→AAT) ybhFYbhF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (79/98);  total (79/98)

Only 100 of 188 total aligned reads displayed.

AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCC  >  NC_000913/826928‑827107
                                                                                          |                                                                                         
aaaaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttc                                                                                         <  2:5751967/93‑1 (MQ=255)
aaaaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttc                                                                                         <  2:3201928/93‑1 (MQ=255)
 aaaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttct                                                                                        >  1:5322812/1‑93 (MQ=255)
  aaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttctc                                                                                       <  2:4426106/93‑1 (MQ=255)
  aaaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAttctc                                                                                       <  1:918688/93‑1 (MQ=255)
   aaGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGCAttctct                                                                                      >  1:4373470/1‑93 (MQ=255)
     gCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCttt                                                                                    <  2:4333920/93‑1 (MQ=255)
     gCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCttt                                                                                    >  1:2987755/1‑93 (MQ=255)
      cGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTc                                                                                   <  1:2218458/93‑1 (MQ=255)
       gTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCa                                                                                  <  2:4241622/93‑1 (MQ=255)
         aaaTTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGa                                                                                <  2:1265252/93‑1 (MQ=255)
         aaaTTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGa                                                                                <  2:837368/93‑1 (MQ=255)
         aaaTTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGa                                                                                <  1:1954881/93‑1 (MQ=255)
          aaTTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGat                                                                               <  2:5022709/93‑1 (MQ=255)
           aTTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatc                                                                              >  1:4092782/1‑93 (MQ=255)
            ttCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatcc                                                                             >  1:4805328/1‑93 (MQ=255)
            ttCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatcc                                                                             <  1:42900/93‑1 (MQ=255)
             tCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatcca                                                                            <  1:2293075/93‑1 (MQ=255)
             tCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatcca                                                                            <  1:2206257/93‑1 (MQ=255)
              cTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatccat                                                                           >  1:539476/1‑93 (MQ=255)
                gTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatccatcc                                                                         >  1:652122/1‑93 (MQ=255)
                gTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGatccatac                                                                         >  1:3338553/1‑91 (MQ=255)
                 ttCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATccc                                                                        >  1:4889311/1‑93 (MQ=255)
                  tCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATcccc                                                                       >  2:2302286/1‑93 (MQ=255)
                   cGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCa                                                                      <  1:5117550/93‑1 (MQ=255)
                    gACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAg                                                                     <  1:3481115/93‑1 (MQ=255)
                    gACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAg                                                                     <  1:3242823/93‑1 (MQ=255)
                     aCCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGc                                                                    <  1:1600800/93‑1 (MQ=255)
                       cGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCAc                                                                  >  2:2263863/1‑93 (MQ=255)
                        gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCAcc                                                                 >  2:4843476/1‑93 (MQ=255)
                        gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCAcc                                                                 <  2:4848791/93‑1 (MQ=255)
                        gTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCAcc                                                                 <  2:4596355/93‑1 (MQ=255)
                          cAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGCTCCATCCCCAGCACCAg                                                               >  2:3790165/1‑93 (MQ=255)
                          cAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAg                                                               <  1:5071154/93‑1 (MQ=255)
                           aGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgc                                                              >  1:5163804/1‑93 (MQ=255)
                           aGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgc                                                              <  2:13714/93‑1 (MQ=255)
                           aGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgc                                                              >  1:551569/1‑93 (MQ=255)
                           aGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgc                                                              >  2:2666741/1‑93 (MQ=255)
                            ggctACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTGTCAGATCCATTCCCAGCACCAgcg                                                             >  2:6183361/4‑93 (MQ=255)
                            ggTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgcg                                                             >  2:5156489/1‑93 (MQ=255)
                            ggTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgcg                                                             >  2:4636988/1‑93 (MQ=255)
                            ggTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAgcg                                                             >  2:5936916/1‑93 (MQ=255)
                                aCCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTg                                                         <  1:3433508/93‑1 (MQ=255)
                                 ccGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTgg                                                        <  2:1270811/93‑1 (MQ=255)
                                 ccGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTgg                                                        >  1:246982/1‑93 (MQ=255)
                                 ccGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTgg                                                        >  2:2555523/1‑93 (MQ=255)
                                   gTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGcc                                                      <  1:6059007/93‑1 (MQ=255)
                                       agCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGaa                                                  <  2:2977999/93‑1 (MQ=255)
                                        gCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGCAg                                                 >  2:1129884/1‑93 (MQ=255)
                                         cGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGt                                                <  1:3629422/93‑1 (MQ=255)
                                          gAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTc                                               >  2:2678009/1‑93 (MQ=255)
                                           aaaaTTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCg                                              <  2:5927193/93‑1 (MQ=255)
                                            aaaTTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCgg                                             <  1:1017396/93‑1 (MQ=255)
                                              aTTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCa                                           >  1:6215783/1‑93 (MQ=255)
                                                ttttGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCAcc                                         <  1:860200/93‑1 (MQ=255)
                                                ttttGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCAcc                                         <  1:607183/93‑1 (MQ=255)
                                                ttttGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCAcc                                         >  2:4173512/1‑93 (MQ=255)
                                                  ttGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAg                                       <  1:1141162/93‑1 (MQ=255)
                                                   tGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGc                                      <  1:627598/93‑1 (MQ=255)
                                                      gcCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCaaa                                   >  2:348240/1‑93 (MQ=255)
                                                        cATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAAcc                                 <  2:3086032/93‑1 (MQ=255)
                                                        cATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCACCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAAcc                                 >  2:5250402/1‑93 (MQ=255)
                                                         atatAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCg                                <  1:5948363/93‑1 (MQ=255)
                                                         atatAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCg                                >  2:1723168/1‑93 (MQ=255)
                                                              gCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcat                           >  1:4202052/1‑93 (MQ=255)
                                                              gCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcat                           >  2:375144/1‑93 (MQ=255)
                                                                cGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCAcatca                         >  2:405416/1‑93 (MQ=255)
                                                                  agaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACatcatc                       >  2:1554591/1‑93 (MQ=255)
                                                                  agaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACatcatc                       >  2:246388/1‑93 (MQ=255)
                                                                   gaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCt                      <  1:2674689/93‑1 (MQ=255)
                                                                   gaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCt                      <  1:1539306/93‑1 (MQ=255)
                                                                   gaTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCt                      <  2:1988414/93‑1 (MQ=255)
                                                                    aTGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCtt                     <  2:5669313/93‑1 (MQ=255)
                                                                     tGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTa                    >  2:2946057/1‑93 (MQ=255)
                                                                      gCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTaa                   <  1:231616/93‑1 (MQ=255)
                                                                       cTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTaaa                  <  1:5564143/93‑1 (MQ=255)
                                                                       cTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTaaa                  >  2:2231532/1‑93 (MQ=255)
                                                                       cTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTaaa                  <  1:4990133/93‑1 (MQ=255)
                                                                       cTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTaaa                  <  1:2112833/93‑1 (MQ=255)
                                                                         ggCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAgg                >  1:2444438/1‑93 (MQ=255)
                                                                         ggCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAgg                >  1:1647469/1‑93 (MQ=255)
                                                                          gcgcgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggt               >  2:67814/1‑93 (MQ=255)
                                                                           cgcgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggtg              <  2:5372629/93‑1 (MQ=255)
                                                                            gcgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggtgg             <  1:5483742/93‑1 (MQ=255)
                                                                            gcgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggtgg             <  1:4006606/93‑1 (MQ=255)
                                                                             cgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggtggt            >  2:3571390/1‑93 (MQ=255)
                                                                             cgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggtggt            <  2:313091/93‑1 (MQ=255)
                                                                             cgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggtggt            <  1:3699229/93‑1 (MQ=255)
                                                                             cgcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGACGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAggcggt            >  2:1778840/1‑93 (MQ=255)
                                                                              gcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTc           <  2:2080321/93‑1 (MQ=255)
                                                                              gcTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTc           <  2:372729/93‑1 (MQ=255)
                                                                               ttttACCGGAATTCTCTTTCAGATCCATCCCCAGCGCGGGCGCCGGGGGGGGGGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCg          <  2:5430729/92‑1 (MQ=255)
                                                                               cTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCg          >  2:2807291/1‑93 (MQ=255)
                                                                                tttACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGTAGTCGGCACCAGCAGACCGCACATCATCTTAAGGGTGGTCgc         >  1:680323/1‑92 (MQ=255)
                                                                                   aCCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGAttt      <  2:3669037/93‑1 (MQ=255)
                                                                                   aCCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGAttt      >  1:1749067/1‑93 (MQ=255)
                                                                                    ccGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTa     <  2:5342535/93‑1 (MQ=255)
                                                                                     cGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTAc    <  1:5717706/93‑1 (MQ=255)
                                                                                      ggAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTAcc   >  1:454922/1‑93 (MQ=255)
                                                                                       gAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACACCATCTTAAAGGTGGTCGATTTAccc  >  2:3939197/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCC  >  NC_000913/826928‑827107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGC  >  NC_000913/826925‑827111
                                                                                             |                                                                                             
GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAAT                                                                                               <  SRR4302533.5133310/94‑1 (MQ=60)
 AGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATT                                                                                              <  SRR4302533.5737676/94‑1 (MQ=60)
   AAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCT                                                                                            >  SRR4302533.5354710/1‑94 (MQ=60)
     AAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGCATTCTCT                                                                                          >  SRR4302533.4399870/1‑94 (MQ=60)
     AAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCT                                                                                          <  SRR4302533.924086/94‑1 (MQ=60)
      AAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTT                                                                                         <  SRR4302533.6122582/94‑1 (MQ=60)
       AGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTT                                                                                        >  SRR4302533.3006032/1‑94 (MQ=60)
       AGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTT                                                                                        >  SRR4302533.670127/1‑94 (MQ=60)
       AGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTT                                                                                        <  SRR4302533.5951040/94‑1 (MQ=60)
         CGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCA                                                                                      <  SRR4302533.2232155/94‑1 (MQ=60)
            AAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGAT                                                                                   <  SRR4302533.5395870/94‑1 (MQ=60)
            AAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGAT                                                                                   <  SRR4302533.1966792/94‑1 (MQ=60)
             AATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATC                                                                                  >  SRR4302533.4117695/1‑94 (MQ=60)
              ATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCC                                                                                 >  SRR4302533.4834425/1‑94 (MQ=60)
               TTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCA                                                                                <  SRR4302533.43192/94‑1 (MQ=60)
               TTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCA                                                                                <  SRR4302533.2924740/94‑1 (MQ=60)
                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               <  SRR4302533.2219863/94‑1 (MQ=60)
                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               <  SRR4302533.2307271/94‑1 (MQ=60)
                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               <  SRR4302533.454582/94‑1 (MQ=60)
                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               >  SRR4302533.542683/1‑94 (MQ=60)
                TCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCAT                                                                               <  SRR4302533.846950/94‑1 (MQ=60)
                  TGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCC                                                                             >  SRR4302533.6287342/1‑94 (MQ=60)
                  TGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCC                                                                             >  SRR4302533.655973/1‑94 (MQ=60)
                  TGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCC                                                                             <  SRR4302533.4865482/94‑1 (MQ=60)
                  TGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCC                                                                             <  SRR4302533.3389789/94‑1 (MQ=60)
                  TGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATAC                                                                             >  SRR4302533.3359187/1‑94 (MQ=60)
                   GTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCC                                                                            >  SRR4302533.4918937/1‑94 (MQ=60)
                      CGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAG                                                                         <  SRR4302533.5148474/94‑1 (MQ=60)
                       GACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGC                                                                        <  SRR4302533.3502588/94‑1 (MQ=60)
                       GACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGC                                                                        >  SRR4302533.1784667/1‑94 (MQ=60)
                       GACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGC                                                                        <  SRR4302533.3262808/94‑1 (MQ=60)
                       GACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGATTTACCGGAATTCTCTTTCAGATCCATCCCCAGC                                                                        >  SRR4302533.4771935/1‑94 (MQ=60)
                        ACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCA                                                                       <  SRR4302533.1610490/94‑1 (MQ=60)
                         CCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCAC                                                                      <  SRR4302533.4249769/94‑1 (MQ=60)
                         CCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCAC                                                                      >  SRR4302533.3605814/1‑94 (MQ=60)
                         CCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCAC                                                                      <  SRR4302533.6016781/94‑1 (MQ=60)
                             CAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGC                                                                  >  SRR4302533.5665275/1‑94 (MQ=60)
                             CAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGC                                                                  >  SRR4302533.554838/1‑94 (MQ=60)
                             CAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGC                                                                  >  SRR4302533.5194983/1‑94 (MQ=60)
                             CAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGC                                                                  <  SRR4302533.5101807/94‑1 (MQ=60)
                              AGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCG                                                                 >  SRR4302533.4100441/1‑94 (MQ=60)
                              AGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCG                                                                 <  SRR4302533.5052940/94‑1 (MQ=60)
                              AGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCG                                                                 >  SRR4302533.2480585/1‑94 (MQ=60)
                              AGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCG                                                                 <  SRR4302533.2360465/94‑1 (MQ=60)
                                GTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCC                                                               >  SRR4302533.37946/1‑94 (MQ=60)
                                  TACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTG                                                             <  SRR4302533.1624630/94‑1 (MQ=60)
                                   ACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGG                                                            <  SRR4302533.3454721/94‑1 (MQ=60)
                                   ACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGG                                                            >  SRR4302533.2553805/1‑94 (MQ=60)
                                   ACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGG                                                            >  SRR4302533.248509/1‑94 (MQ=60)
                                    CCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGC                                                           >  SRR4302533.2248217/1‑94 (MQ=60)
                                      GTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCG                                                         <  SRR4302533.6095688/94‑1 (MQ=60)
                                       TAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGG                                                        >  SRR4302533.4186329/1‑94 (MQ=60)
                                            CGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTC                                                   <  SRR4302533.3651754/94‑1 (MQ=60)
                                               AAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGC                                                <  SRR4302533.1023376/94‑1 (MQ=60)
                                               AAATTTTTGCGCCATATAGCCGAGATACTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGC                                                <  SRR4302533.5131898/94‑1 (MQ=60)
                                                AATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCA                                               >  SRR4302533.6253055/1‑94 (MQ=60)
                                                   TTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCA                                            <  SRR4302533.610755/94‑1 (MQ=60)
                                                   TTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCA                                            <  SRR4302533.865241/94‑1 (MQ=60)
                                                     TTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGC                                          <  SRR4302533.6321164/94‑1 (MQ=60)
                                                     TTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGC                                          <  SRR4302533.1147960/94‑1 (MQ=60)
                                                      TGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCA                                         <  SRR4302533.631287/94‑1 (MQ=60)
                                                          CCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACC                                     <  SRR4302533.2327358/94‑1 (MQ=60)
                                                          CCATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACC                                     <  SRR4302533.2129008/94‑1 (MQ=60)
                                                            ATATAGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGC                                   <  SRR4302533.5984341/94‑1 (MQ=60)
                                                                AGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACAT                               >  SRR4302533.1575722/1‑94 (MQ=60)
                                                                AGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACAT                               <  SRR4302533.1250283/94‑1 (MQ=60)
                                                                AGCCGAGATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACAT                               >  SRR4302533.4227475/1‑94 (MQ=60)
                                                                      GATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTT                         <  SRR4302533.2691093/94‑1 (MQ=60)
                                                                      GATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTT                         <  SRR4302533.1548575/94‑1 (MQ=60)
                                                                       ATGCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTA                        <  SRR4302533.5501633/94‑1 (MQ=60)
                                                                         GCTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAA                      <  SRR4302533.233047/94‑1 (MQ=60)
                                                                          CTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAG                     <  SRR4302533.5597773/94‑1 (MQ=60)
                                                                          CTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAG                     <  SRR4302533.5020386/94‑1 (MQ=60)
                                                                          CTGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAG                     <  SRR4302533.2125866/94‑1 (MQ=60)
                                                                           TGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGG                    <  SRR4302533.869813/94‑1 (MQ=60)
                                                                           TGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGG                    >  SRR4302533.1657447/1‑94 (MQ=60)
                                                                           TGGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGG                    >  SRR4302533.2459526/1‑94 (MQ=60)
                                                                            GGCGCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGT                   <  SRR4302533.3668047/94‑1 (MQ=60)
                                                                               GCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGT                <  SRR4302533.3095896/94‑1 (MQ=60)
                                                                               GCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGT                <  SRR4302533.5516794/94‑1 (MQ=60)
                                                                               GCGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGT                <  SRR4302533.4030999/94‑1 (MQ=60)
                                                                                CGCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTC               <  SRR4302533.3721948/94‑1 (MQ=60)
                                                                                 GCTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCG              >  SRR4302533.4925606/1‑94 (MQ=60)
                                                                                  CTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGTAGTCGGCACCAGCAGACCGCACATCATCTTAAGGGTGGTCGC             >  SRR4302533.684348/1‑94 (MQ=60)
                                                                                  CTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGA             >  SRR4302533.1732379/1‑94 (MQ=60)
                                                                                  CTTTACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGA             >  SRR4302533.5538916/1‑94 (MQ=60)
                                                                                     TACCGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTT          >  SRR4302533.1759716/1‑94 (MQ=60)
                                                                                        CGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACC       <  SRR4302533.5752237/94‑1 (MQ=60)
                                                                                        CGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACC       <  SRR4302533.557591/94‑1 (MQ=60)
                                                                                        CGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACC       >  SRR4302533.5192532/1‑94 (MQ=60)
                                                                                        CGGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACC       >  SRR4302533.457639/1‑94 (MQ=60)
                                                                                         GGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCC      <  SRR4302533.2116885/94‑1 (MQ=60)
                                                                                         GGAATTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCC      <  SRR4302533.3461770/94‑1 (MQ=60)
                                                                                             TTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGC  >  SRR4302533.3141470/1‑94 (MQ=60)
                                                                                             TTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGC  <  SRR4302533.4085050/94‑1 (MQ=60)
                                                                                             |                                                                                             
GAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGAGCGAAAATTTTTGCGCCATATAGCCGAGATGCTGGCGCGCTTTACCGGAACTCTCTTTCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGC  >  NC_000913/826925‑827111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑